CN110432485A - Dendrobium candidum fermentation liquid, drink and preparation method and purposes - Google Patents
Dendrobium candidum fermentation liquid, drink and preparation method and purposes Download PDFInfo
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- CN110432485A CN110432485A CN201910751479.5A CN201910751479A CN110432485A CN 110432485 A CN110432485 A CN 110432485A CN 201910751479 A CN201910751479 A CN 201910751479A CN 110432485 A CN110432485 A CN 110432485A
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- dendrobium candidum
- fermentation liquid
- liquid
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Links
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/231—Lactis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/31—Leuconostoc
- A23V2400/321—Mesenteroides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Non-Alcoholic Beverages (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of dendrobium candidum fermentation liquid, drink and preparation method and purposes.The dendrobium candidum fermentation liquid is by including that the raw material of following component is prepared: 400~1600 parts by weight dendrobium candidums, 400~1200 parts by weight kiwi-fruit juices, 40~400 parts by weight sugarcane inspissated juices, 0.08~0.8 parts by weight zymophyte powder and 8~64 parts by weight enzymolysis agents.Dendrobium candidum fermentation liquid of the invention and drink all have very significant nourishing Yin and clearing heat effect.
Description
Technical field
The present invention relates to a kind of dendrobium candidum fermentation liquid, drink and preparation method and purposes, more particularly to have enriching yin clear
Dendrobium candidum fermentation liquid, drink and the preparation method and purposes of heat effect.
Background technique
Dendrobium candidum is orchid family dendrobium perennial grass plant, and happiness is raw in warm, moist, half yin and half yang environment
It is long.Dendrobium candidum is distributed mainly on the ground such as Fujian, Zhejiang, Guangxi, Yunnan.Dendrobium candidum be dendrobium nobile superfine product, it is rare it is rare in
Medicinal material, it gains the name because epidermis is green in iron.Dendrobium candidum has unique medical value, belongs to the yin tonics in tonic, tool
There is a reinforcing stomach reg fluid, nourishing Yin and clearing heat, tonifying five zang organs consumptive disease, internal organ are insufficient, and the effect of nourishing the liver to improve visual acuity and antifatigue, eliminating phlegm and relieving cough etc. are made
With.Dendrobium candidum, which also has, to be improved immunity, protection stomach lining, promotes the pharmacological actions such as digestion, anti-aging, can be very good to change
The sub-health state of kind contemporary people.
CN104585762B discloses a kind of dendrobium candidum fermented product and preparation method thereof.This method includes following step
It is rapid: 1) that by the broken rear extracting in water of dendrobium candidum, extracting solution is made；2) after medium component being added into extracting solution, intestines are accessed
Film leukonid carries out the first fermentation, and the first fermentation liquid is made；3) to pH value be 4.5~6 fruits and vegetables liquid in be added pectase into
Enzymatic hydrolysis fruits and vegetables liquid is made in row enzymolysis processing；4) after medium component being added into enzymatic hydrolysis fruits and vegetables liquid, access compound lactobacillus is carried out
Second fermentation, is made the second fermentation liquid；5) after medium component being added into the second fermentation liquid, access mulriple yeasts carry out the
Three fermentations, are made third fermentation liquid；6) will the first fermentation liquid and third fermentation liquid mix after be centrifuged, to centrifuged supernatant homogeneous,
After sterilizing, dendrobium candidum fermented product is made.Dendrobium candidum fermented product prepared by the present invention is in good taste, unique flavor, has
Good immunological regulation and functions, the target user such as anti-oxidant are in extensive range.The dendrobium candidum fermented product of above patent document
Not by allotment, mouthfeel is not good enough and nourishing Yin and clearing heat effect is not significant.
CN108308468A discloses a kind of with defaecation, the dendrobium candidum enzyme beverage of stomach-nourishing function and its preparation side
Method.The beverage includes that following method is prepared: being cut into 0.5cm segment after 1. selecting fresh dendrobium candidum to clear up；2. will
The dendrobium candidum section and pure water of weight ratio 1:10~30 mix, colloid mill mashing；3. above-mentioned slurry heat sterilization, condition be 50~
85 DEG C, 10~30min；4. lactobacillus-fermented is added in above-mentioned slurry, condition is 35~45 DEG C, 12~72h；5. above-mentioned slurry passes through
Centrifugal filtration obtains fermentation liquid；6. beverage ingredient is as follows: 100~400 parts of fermentation liquid, 10~30 parts of honey, oligofructose 7~15
Part, 5~10 parts of resistant dextrin, 4~8 parts of concentrated apple juice, 0.3~1 part of xanthan gum, 0.2~0.6 part of CMC, water 600~900
Part；7. above-mentioned beverage passes through high pressure homogenizer, condition is 2~30MPa；8. above-mentioned beverage aseptically sterilizes and filling.
Beneficial effect of the present invention: beverage is arranged in pairs or groups after natural fermented with dietary fiber, and not only mouthfeel is full, and has good support
Stomach, aperient effects.The raw material of the fermentation liquid of above patent document is relatively simple and without enzymatic hydrolysis, cause dendrobium candidum it is effective at
Divide dissolution insufficient, the utilization rate of dendrobium candidum reduces, and the nourishing Yin and clearing heat effect of fermentation liquid is not significant.
Summary of the invention
In view of this, the first purpose of this invention is to provide a kind of dendrobium candidum fermentation liquid, nourishing Yin and clearing heat effect
It is very significant.
Second object of the present invention is to provide the preparation method of above-mentioned dendrobium candidum fermentation liquid, can greatly improve
The utilization rate of dendrobium candidum.
Third object of the present invention is to provide a kind of drink, containing above-mentioned dendrobium candidum fermentation liquid and Cordyceps militaris,
Asparagus juice etc., not only mouthfeel is good and nourishing Yin and clearing heat effect is very significant.
Fourth object of the present invention is to provide the preparation method of above-mentioned drink, dendrobium candidum fermentation liquid and Cordyceps militaris,
Asparagus juice etc. acts synergistically in allocation process, so that not only mouthfeel is good and nourishing Yin and clearing heat effect is very significant for drink.
Of the invention the 5th is designed to provide the purposes of above-mentioned dendrobium candidum fermentation liquid or drink.
The present invention adopts the following technical scheme that realization above-mentioned purpose.
On the one hand, the present invention provides a kind of dendrobium candidum fermentation liquid, is prepared by the raw material for including following component:
400~1600 parts by weight dendrobium candidums, 400~1200 parts by weight kiwi-fruit juices, 40~400 parts by weight sugarcane inspissated juices, 0.08
~0.8 parts by weight zymophyte powder and 8~64 parts by weight enzymolysis agents.
Dendrobium candidum fermentation liquid according to the present invention, it is preferable that the zymophyte powder includes 0.04~0.4 parts by weight goldbeater's skin
Leukonid and 0.04~0.4 parts by weight Lactococcus lactis；And the enzymolysis agent include 4~32 part by weight of cellulose enzymes and 4~
32 parts by weight pectases.
Dendrobium candidum fermentation liquid according to the present invention, it is preferable that the raw material further includes 40~400 parts by weight white granulated sugars, 4
~20 parts by weight wheat oligopeptides, 4~20 part by weight of vitamin C and 4~40 parts by weight sodium bicarbonates.
Dendrobium candidum fermentation liquid according to the present invention, it is preferable that it is by including that the raw material of following component is prepared: 600
~1400 parts by weight dendrobium candidums, 400~800 parts by weight kiwi-fruit juices, 100~400 parts by weight sugarcane inspissated juices, 60~300
Parts by weight white granulated sugar, 6~15 parts by weight wheat oligopeptides, 6~18 part by weight of vitamin C, 8~30 parts by weight sodium bicarbonates, 0.2
~0.7 parts by weight zymophyte powder and 20~50 parts by weight enzymolysis agents.
On the other hand, the present invention provides the preparation method of above-mentioned dendrobium candidum fermentation liquid comprising following steps:
1) it is beaten: dendrobium candidum is subjected to crushing and beating, obtain dendrobium candidum slurry；
2) it digests: enzymolysis agent is added in Xiang Suoshu dendrobium candidum slurry, is warming up to 40~60 DEG C, digests 60~120min, obtains
To enzymolysis liquid；
3) mixingization sugar: in Xiang Suoshu enzymolysis liquid be added white granulated sugar, wheat oligopeptide, kiwi-fruit juice, sugarcane inspissated juice and
Sodium bicarbonate is uniformly mixed, and system pH is adjusted to 6.0~6.2, mixed liquor is obtained；
4) it sterilizes: the mixed liquor being kept the temperature into 30~90min under the conditions of temperature is 80~100 DEG C, obtains sterilized solution；
5) it ferments: the sterilized solution is cooled to 20~28 DEG C, be added zymophyte powder or zymophyte powder solution, fermentation 60~
96h obtains fermentation liquid；
6) it filters: the fermentation liquid being filtered to obtain fermentation clear liquid, the as described dendrobium candidum fermentation liquid.
Another aspect, the present invention provide a kind of dendrobium candidum fermented beverage comprising blender and iron sheet as described above
The weight ratio of dendrobium nobile fermentation liquid, the dendrobium candidum fermentation liquid and the blender is 10~40:0.825~14.1.
Dendrobium candidum fermented beverage according to the present invention, it is preferable that the blender include 0.1~2 parts by weight Cordyceps militaris,
0.1~0.5 parts by weight of lemon acid sodium, 0.5~3 parts by weight antierythrite, 0.005~0.1 parts by weight acesulfame potassium, 0.01~0.5
Parts by weight xanthan gum, 0.01~0.1 parts by weight essence and 0.1~3 parts by weight aloe gel inspissated juice.
Dendrobium candidum fermented beverage according to the present invention, it is preferable that it is by including that the raw material of following component is made: 15~35
Parts by weight dendrobium candidum fermentation liquid, 0.3~1.5 parts by weight Cordyceps militaris, 0.15~0.4 parts by weight of lemon acid sodium, 0.6~2 weight
Part antierythrite, 0.008~0.08 parts by weight acesulfame potassium, 0.03~0.3 parts by weight xanthan gum, 0.015~0.08 parts by weight are fragrant
Essence and 0.2~2 parts by weight aloe gel inspissated juice.
Another aspect, the present invention provide the preparation method of above-mentioned dendrobium candidum fermented beverage comprising following steps:
(1) premixization glue: xanthan gum, antierythrite and sodium citrate are premixed, rubber powder is obtained；The rubber powder puts into water
In, 10~30min is stirred, glue is obtained, dendrobium candidum fermentation liquid is added, mixes, obtains the first mixed liquor；
(2) material is deployed: essence, acesulfame potassium, aloe gel inspissated juice and Cordyceps militaris are added to first mixed liquor
In, continue 20~40min of stirring, water constant volume is added to obtain the second mixed liquor；Second mixed liquor is filtered, seasoning liquid is obtained；
(3) it sterilizes: the seasoning liquid being de-gassed, homogeneous and sterilizing, sterilising temp are 132~137 DEG C, sterilization time
For 4~10s, the dendrobium candidum fermented beverage is obtained.
In another aspect, the present invention, which provides above-mentioned dendrobium candidum fermentation liquid, is used to prepare the health care product with nourishing Yin and clearing heat effect
Or the purposes of drink.
Dendrobium candidum fermentation liquid of the invention has very significant nourishing Yin and clearing heat effect.Dendrobium candidum fermentation of the invention
The preparation method of liquid can greatly improve the utilization rate of dendrobium candidum.Drink of the invention contain above-mentioned dendrobium candidum fermentation liquid with
Cordyceps militaris, asparagus juice etc., not only mouthfeel is good and nourishing Yin and clearing heat effect is very significant.The preparation method of drink of the invention, iron sheet
Dendrobium nobile fermentation liquid and Cordyceps militaris, asparagus juice etc. act synergistically in allocation process, so that dendrobium candidum fermented beverage not only mouthfeel
Good and nourishing Yin and clearing heat effect is very significant.
Detailed description of the invention
Fig. 1 is the viable count monitoring result of fermentation liquid in the fermentation process of embodiment 1.
Fig. 2 is the total acid monitoring result of fermentation liquid in the fermentation process of embodiment 1.
Fig. 3 is the pH value monitoring result of fermentation liquid in the fermentation process of embodiment 1.
Fig. 4 is the Thick many candies monitoring result of fermentation liquid in the fermentation process of embodiment 1.
Specific embodiment
The present invention is further illustrated combined with specific embodiments below, but protection scope of the present invention is not limited to
This.
< dendrobium candidum fermentation liquid and preparation method >
Dendrobium candidum fermentation liquid is by including that the raw material of following component is prepared: dendrobium candidum, kiwi-fruit juice, sugarcane are concentrated
Juice, zymophyte powder and enzymolysis agent.It optionally, can also include white granulated sugar, wheat oligopeptide, vitamin C, sodium bicarbonate and water.
Some specific embodiments according to the present invention, dendrobium candidum fermentation liquid are prepared by the raw material of following component: dendrobium candidum,
Kiwi-fruit juice, sugarcane inspissated juice, zymophyte powder, enzymolysis agent, white granulated sugar, wheat oligopeptide, vitamin C and sodium bicarbonate, surplus
For water.
In the present invention, dendrobium candidum can be dendrobium candidum leaf or dendrobium candidum stem, preferably dendrobium candidum fresh leaf or
The fresh stem of dendrobium candidum, more preferably dendrobium candidum fresh leaf.Water can be any drinkable water, preferably pure water.
In the present invention, dendrobium candidum fermentation liquid is by including that the raw material of following component is prepared: dendrobium candidum can be
400~1600 parts by weight, preferably 600~1400 parts by weight, more preferably 900~1200 parts by weight；Kiwi-fruit juice can be
400~1200 parts by weight, preferably 400~800 parts by weight, more preferably 400~600 parts by weight；Sugarcane inspissated juice can be
40~400 parts by weight, preferably 100~400 parts by weight, more preferably 200~400 parts by weight；Zymophyte powder can be 0.08
~0.8 parts by weight, preferably 0.2~0.7 parts by weight, more preferably 0.4~0.6 parts by weight；Enzymolysis agent can be 8~64 weight
Part, preferably 20~50 parts by weight, more preferably 30~40 parts by weight.Each component is with said ratio compatibility, so that dendrobium candidum
Fermentation liquid has very significant nourishing Yin and clearing heat effect.
In the present invention, the raw material can also include: white granulated sugar can for 40~400 parts by weight, preferably 60~300
Parts by weight, more preferably 80~200 parts by weight；Wheat oligopeptide can be 4~20 parts by weight, preferably 6~15 parts by weight, more
Preferably 8~12 parts by weight；Vitamin C can be 4~20 parts by weight, preferably 6~18 parts by weight, more preferably 8~15 weights
Measure part；Sodium bicarbonate can be 4~40 parts by weight, preferably 8~30 parts by weight, more preferably 10~20 parts by weight.
Dendrobium candidum fermentation liquid according to the present invention, it is preferable that dendrobium candidum fermentation liquid is by the raw material including following component
Be prepared: 400~1600 parts by weight dendrobium candidums, 400~1200 parts by weight kiwi-fruit juices, 40~400 parts by weight sugarcanes are dense
Contracting juice, 0.08~0.8 parts by weight zymophyte powder and 8~64 parts by weight enzymolysis agents.
Dendrobium candidum fermentation liquid according to the present invention, it is preferable that dendrobium candidum fermentation liquid is by the raw material including following component
Be prepared: 400~1600 parts by weight dendrobium candidums, 400~1200 parts by weight kiwi-fruit juices, 40~400 parts by weight sugarcanes are dense
Contracting juice, 0.08~0.8 parts by weight zymophyte powder, 8~64 parts by weight enzymolysis agents, 40~400 parts by weight white granulated sugars, 4~20 weight
Part wheat oligopeptide, 4~20 part by weight of vitamin C and 4~40 parts by weight sodium bicarbonates.A specific implementation according to the present invention
Mode, by dendrobium candidum fermentation liquid be 4000 parts by weight in terms of, dendrobium candidum fermentation liquid is prepared by the raw material of following component:
400~1600 parts by weight dendrobium candidums, 400~1200 parts by weight kiwi-fruit juices, 40~400 parts by weight sugarcane inspissated juices, 0.08
~0.8 parts by weight zymophyte powder, 8~64 parts by weight enzymolysis agents, 40~400 parts by weight white granulated sugars, 4~20 parts by weight wheats are oligomeric
Peptide, 4~20 part by weight of vitamin C, 4~40 parts by weight sodium bicarbonates, surplus is water.
Dendrobium candidum fermentation liquid according to the present invention, it is preferable that dendrobium candidum fermentation liquid is by the raw material including following component
Be prepared: 600~1400 parts by weight dendrobium candidums, 400~800 parts by weight kiwi-fruit juices, 100~400 parts by weight sugarcanes are dense
Contracting juice, 0.2~0.7 parts by weight zymophyte powder, 20~50 parts by weight enzymolysis agents, 60~300 parts by weight white granulated sugars, 6~15 weight
Part wheat oligopeptide, 6~18 part by weight of vitamin C and 8~30 parts by weight sodium bicarbonates.A specific implementation according to the present invention
Mode, by dendrobium candidum fermentation liquid be 4000 parts by weight in terms of, dendrobium candidum fermentation liquid is prepared by the raw material of following component:
600~1400 parts by weight dendrobium candidums, 400~800 parts by weight kiwi-fruit juices, 100~400 parts by weight sugarcane inspissated juices, 0.2~
0.7 parts by weight zymophyte powder, 20~50 parts by weight enzymolysis agents, 60~300 parts by weight white granulated sugars, 6~15 parts by weight wheats are oligomeric
Peptide, 6~18 part by weight of vitamin C, 8~30 parts by weight sodium bicarbonates, surplus is water.
Dendrobium candidum fermentation liquid according to the present invention, it is preferable that dendrobium candidum fermentation liquid is by the raw material including following component
Be prepared: 900~1200 parts by weight dendrobium candidums, 400~600 parts by weight kiwi-fruit juices, 200~400 parts by weight sugarcanes are dense
Contracting juice, 0.4~0.6 parts by weight zymophyte powder, 30~40 parts by weight enzymolysis agents, 80~200 parts by weight white granulated sugars, 8~12 weight
Part wheat oligopeptide, 8~15 part by weight of vitamin C and 10~20 parts by weight sodium bicarbonates.One according to the present invention specific real
Apply mode, by dendrobium candidum fermentation liquid be 4000 parts by weight in terms of, dendrobium candidum fermentation liquid is prepared by the raw material of following component:
900~1200 parts by weight dendrobium candidums, 400~600 parts by weight kiwi-fruit juices, 200~400 parts by weight sugarcane inspissated juices, 0.4~
0.6 parts by weight zymophyte powder, 30~40 parts by weight enzymolysis agents, 80~200 parts by weight white granulated sugars, 8~12 parts by weight wheats are oligomeric
Peptide, 8~15 part by weight of vitamin C, 10~20 parts by weight sodium bicarbonates, surplus is water.
In the present invention, zymophyte powder can be selected from Leuconostoc mesenteroides, Lactococcus lactis, bacillus coagulans, long pair
One of discrimination bacillus, bifidobacterium lactis, streptococcus thermophilus, lactobacillus plantarum, Lactobacillus casei, Lactobacillus rhamnosus are more
Kind；Preferably one of Leuconostoc mesenteroides, Lactococcus lactis, bacillus coagulans, bifidobacterium longum, bifidobacterium lactis or
It is a variety of；More preferably Leuconostoc mesenteroides and Lactococcus lactis.Certain specific embodiments according to the present invention, zymophyte powder are
Leuconostoc mesenteroides and Lactococcus lactis；Leuconostoc mesenteroides is 0.04~0.4 parts by weight；Lactococcus lactis is 0.04~0.4
Parts by weight.Preferably, Leuconostoc mesenteroides is 0.1~0.35 parts by weight；Lactococcus lactis is 0.1~0.35 parts by weight.It is more excellent
Selection of land, Leuconostoc mesenteroides are 0.2~0.3 parts by weight；Lactococcus lactis is 0.2~0.3 parts by weight.Leuconostoc mesenteroides has
Imitating bacterial content can be 1000~200,000,000,000 CFU/g, preferably 1500~200,000,000,000 CFU/g.Effective bacterium of Lactococcus lactis contains
Amount can be 1000~250,000,000,000 CFU/g, preferably 1800~230,000,000,000 CFU/g.Using Leuconostoc mesenteroides and Lactococcus lactis
Thick many candies content had both can be improved in bacterium, can also reduce acidity and make mouthfeel good.
In the present invention, enzymolysis agent can be cellulase or pectase, preferably cellulase and pectase.According to this
Certain specific embodiments of invention, enzymolysis agent are cellulase and pectase；Cellulase is 4~32 parts by weight；Pectase
For 4~32 parts by weight.Preferably, cellulase is 10~25 parts by weight, and pectase is 10~25 parts by weight.It is highly preferred that fine
Tieing up plain enzyme is 15~20 parts by weight；Pectase is 15~20 parts by weight.The enzyme activity of cellulase be can be 1~30,000 U/g,
Preferably 2~30,000 U/g.The enzyme activity of pectase can be 1~30,000 U/g, preferably 2~30,000 U/g.Using pectase and fibre
The cellulose that tieing up plain enzyme can decompose in pectin and degradation cell wall reduces so that nutritional ingredient more thoroughly discharges
The viscosity of material is conducive to mass transfer and heat transfer in subsequent fermentations, so that fermentation process is more stable.
In the present invention, soluble solid content Brix (%) value of kiwi-fruit juice is preferably greater than to be equal to 12.Sugarcane
Soluble solid content Brix (%) value of inspissated juice is preferably greater than to be equal to 64.In white granulated sugar cane sugar content preferably greater than etc.
In 98wt%.The mesh number of white granulated sugar is preferably 30~80 mesh.Wheat oligopeptide meets new resource food requirement；In wheat oligopeptide
Oligomeric peptide content is preferably greater than or equal to 75wt%.Vitamin C is food-grade.Ascorbic purity is preferably greater than to be equal to
99wt%.Ascorbic main function is to prevent dendrobium candidum from brown stain occurring in pulping process.Sodium bicarbonate is food-grade.
The mesh number of sodium bicarbonate is preferably 30~80 mesh.
The preparation method of above-mentioned dendrobium candidum fermentation liquid is described below comprising following steps: 1) being beaten；2) it digests；3)
Mixingization sugar；4) it sterilizes；5) it ferments；6) it filters.
In step 1), dendrobium candidum is subjected to crushing and beating, obtains dendrobium candidum slurry.Vitamin can be added when mashing
C is to prevent brown stain from aoxidizing.The mesh number of dendrobium candidum slurry ranges preferably from 40~100 mesh.The dosage of each component is as described above, here
It repeats no more.
In step 2), enzymolysis agent is added in Xiang Suoshu dendrobium candidum slurry, is warming up to 40~60 DEG C, enzymatic hydrolysis 60~
120min obtains enzymolysis liquid.It is preferred that being warming up to 50 ± 3 DEG C.The time of enzymatic hydrolysis is preferably 85~100min, more preferably 85~
95min.Enzymolysis agent is as described above, which is not described herein again.
In step 3), white granulated sugar, wheat oligopeptide, kiwi-fruit juice, sugarcane inspissated juice and carbonic acid are added into enzymolysis liquid
Hydrogen sodium is uniformly mixed, and obtains mixed liquor.The pH of mixed liquor is 6.0~6.2.The dosage of each component is as described above, no longer superfluous here
It states.
In step 4), the mixed liquor is kept the temperature into 30~90min under the conditions of temperature is 80~100 DEG C, is sterilized
Liquid.The mixed liquor is preferably kept the temperature under the conditions of temperature is 85~95 DEG C.Soaking time is preferably 20~40min, more preferably
25~35min.
In step 5), sterilized solution is cooled to 20~28 DEG C, is added zymophyte powder or zymophyte powder solution, fermentation 60~
96h obtains fermentation liquid.Fermentation time is preferably 65~80h, more preferably 70~73h.The zymophyte powder solution can be by weight
It measures than being that the zymophyte powder of 1:5~20 is prepared to obtain with water, is preferably matched by zymophyte powder and water that weight ratio is 1:8~15 and be made
It arrives, more preferably prepares to obtain with water by the zymophyte powder that weight ratio is 1:9~11.Zymophyte powder is as described above, no longer superfluous here
It states.
In step 6), fermentation liquid is filtered to obtain fermentation clear liquid, the as described dendrobium candidum fermentation liquid.Filtering is set
Standby preferably 40 mesh, 60 mesh cloth envelop collectors.
1) specific embodiment according to the present invention, the preparation method of dendrobium candidum fermentation liquid include the following steps:
Mashing: carrying out crushing and beating for dendrobium candidum, obtains dendrobium candidum slurry；2) it digests: enzymatic hydrolysis is added in Xiang Suoshu dendrobium candidum slurry
Agent is warming up to 40~60 DEG C, digests 60~120min, obtains enzymolysis liquid；3) white sand mixingization sugar: is added in Xiang Suoshu enzymolysis liquid
Sugar, wheat oligopeptide, kiwi-fruit juice, sugarcane inspissated juice and sodium bicarbonate are uniformly mixed, and obtain mixed liquor；4) it sterilizes: will be described
Mixed liquor keeps the temperature 30~90min under the conditions of temperature is 80~100 DEG C, obtains sterilized solution；5) it ferments: the sterilized solution is cooled down
To 20~28 DEG C, zymophyte powder is added, ferment 60~96h, obtains fermentation liquid；Wherein, the zymophyte powder is by the bright beading of goldbeater's skin
Bacterium and Lactococcus lactis composition；6) it filters: the fermentation liquid being filtered to obtain fermentation clear liquid, the as described dendrobium candidum hair
Zymotic fluid.
The preparation method of another embodiment according to the present invention, dendrobium candidum fermentation liquid includes the following steps:
1) it is beaten: dendrobium candidum is subjected to crushing and beating, obtain dendrobium candidum slurry；2) it digests: enzyme is added in Xiang Suoshu dendrobium candidum slurry
Agent is solved, is warming up to 40~60 DEG C, 60~120min is digested, obtains enzymolysis liquid；3) it mixingization sugar: is added in Xiang Suoshu enzymolysis liquid white
Granulated sugar, wheat oligopeptide, kiwi-fruit juice, sugarcane inspissated juice and sodium bicarbonate are uniformly mixed, and obtain mixed liquor；4) it sterilizes: by institute
It states mixed liquor and keeps the temperature 30~90min under the conditions of temperature is 80~100 DEG C, obtain sterilized solution；5) it ferments: the sterilized solution is dropped
Zymophyte powder or zymophyte powder solution is added to 20~28 DEG C in temperature, and ferment 60~96h, obtains fermentation liquid；Wherein, the fermentation
Bacterium powder solution is prepared to obtain by the zymophyte powder that weight ratio is 1:5~20 with water；The zymophyte powder by Leuconostoc mesenteroides and
Lactococcus lactis composition；6) it filters: the fermentation liquid being filtered to obtain fermentation clear liquid, the as described dendrobium candidum fermentation
Liquid.
< dendrobium candidum fermented beverage and preparation method >
Dendrobium candidum fermented beverage is by including that the raw material of following component is prepared: dendrobium candidum fermentation liquid and blender.
In the present invention, the weight ratio of dendrobium candidum fermentation liquid and the blender can for 10~40:0.825~
14.1, preferably 15~35:1.303~6.36, more preferably 20~30:2.08~4.01.
In the present invention, the blender includes Cordyceps militaris, sodium citrate, antierythrite, acesulfame potassium, xanthan gum, essence
With aloe gel inspissated juice.It optionally, can also include other auxiliary materials.Some specific embodiments according to the present invention,
Dendrobium candidum fermented beverage is made of the raw material of following component: dendrobium candidum fermentation liquid, Cordyceps militaris, sodium citrate, antierythrite,
Acesulfame potassium, xanthan gum, essence, aloe gel inspissated juice, surplus are water.
In the present invention, dendrobium candidum fermented beverage is by including that the raw material of following component is made: dendrobium candidum fermentation liquid can
Think 10~40 parts by weight, preferably 15~35 parts by weight, more preferably 20~30 parts by weight；Cordyceps militaris can be 0.1~2 weight
Measure part, preferably 0.3~1.5 parts by weight, more preferably 0.5~1 parts by weight；Sodium citrate can be 0.1~0.5 parts by weight,
Preferably 0.15~0.4 parts by weight, more preferably 0.2~0.3 parts by weight；Antierythrite can be 0.5~3 parts by weight, preferably
For 0.6~2 parts by weight, more preferably 0.8~1.5 parts by weight；Acesulfame potassium can be 0.005~0.1 parts by weight, preferably
0.008~0.08 parts by weight, more preferably 0.01~0.05 parts by weight；Xanthan gum can be 0.01~0.5 parts by weight, preferably
0.03~0.3 parts by weight, more preferably 0.05~0.1 parts by weight；Essence can be 0.01~0.1 parts by weight, preferably 0.015
~0.08 parts by weight, more preferably 0.02~0.06 parts by weight；Aloe gel inspissated juice can be 0.1~3 parts by weight, preferably
0.2~2 parts by weight, more preferably 0.5~1 parts by weight.Each component is with said ratio compatibility, dendrobium candidum fermentation liquid and pupa worm
Grass, asparagus juice etc. act synergistically in allocation process, and not only mouthfeel is good and nourishing Yin and clearing heat acts on for gained dendrobium candidum fermented beverage
It is very significant.
Dendrobium candidum fermented beverage according to the present invention, it is preferable that dendrobium candidum fermented beverage is by including following component
Raw material is made: 10~40 parts by weight dendrobium candidum fermentation liquids, 0.1~2 parts by weight Cordyceps militaris, 0.1~0.5 parts by weight of lemon acid
Sodium, 0.5~3 parts by weight antierythrite, 0.005~0.1 parts by weight acesulfame potassium, 0.01~0.5 parts by weight xanthan gum, 0.01~
0.1 parts by weight essence and 0.1~3 parts by weight aloe gel inspissated juice.A specific embodiment according to the present invention, with iron sheet
Dendrobium nobile fermented beverage is 50ml meter, and dendrobium candidum fermented beverage is made of the raw material of following component: the fermentation of 10~40g dendrobium candidum
Liquid, 0.1~2g Cordyceps militaris, 0.1~0.5g sodium citrate, 0.5~3g antierythrite, 0.005~0.1g acesulfame potassium, 0.01~
0.5g xanthan gum, 0.01~0.1g essence, 0.1~3g aloe gel inspissated juice, surplus is water.
Dendrobium candidum fermented beverage according to the present invention, it is preferable that dendrobium candidum fermented beverage is by including following component
Raw material is made: 15~35 parts by weight dendrobium candidum fermentation liquids, 0.3~1.5 parts by weight Cordyceps militaris, 0.15~0.4 parts by weight of lemon
Sour sodium, 0.6~2 parts by weight antierythrite, 0.008~0.08 parts by weight acesulfame potassium, 0.03~0.3 parts by weight xanthan gum, 0.015
~0.08 parts by weight essence and 0.2~2 parts by weight aloe gel inspissated juice.A specific embodiment according to the present invention, with
Dendrobium candidum fermented beverage is 50ml meter, and dendrobium candidum fermented beverage is made of the raw material of following component: 15~35g dendrobium candidum
Fermentation liquid, 0.3~1.5g Cordyceps militaris, 0.15~0.4g sodium citrate, 0.6~2g antierythrite, 0.008~0.08g acesulfame potassium,
0.03~0.3g xanthan gum, 0.015~0.08g essence, 0.2~2g aloe gel inspissated juice, surplus is water.
Dendrobium candidum fermented beverage according to the present invention, it is preferable that dendrobium candidum fermented beverage is by including following component
Raw material is made: 20~30 parts by weight dendrobium candidum fermentation liquids, 0.5~1 parts by weight Cordyceps militaris, 0.2~0.3 parts by weight of lemon acid
Sodium, 0.8~1.5 parts by weight antierythrite, 0.01~0.05 parts by weight acesulfame potassium, 0.05~0.1 parts by weight xanthan gum, 0.02~
0.06 parts by weight essence and 0.5~1 parts by weight aloe gel inspissated juice.A specific embodiment according to the present invention, with iron
Skin dendrobium nobile fermented beverage is 50ml meter, and dendrobium candidum fermented beverage is made of the raw material of following component: 20~30g dendrobium candidum hair
Zymotic fluid, 0.5~1g Cordyceps militaris, 0.2~0.3g sodium citrate, 0.8~1.5g antierythrite, 0.01~0.05g acesulfame potassium, 0.05
~0.1g xanthan gum, 0.02~0.06g essence, 0.5~1g aloe gel inspissated juice, surplus is water.
In the present invention, Cordyceps militaris is new resource food, contains polysaccharide.Sodium citrate can be used as deacidification substance, use
In the acidity for reducing dendrobium candidum fermented beverage.Aloe gel inspissated juice is new resource food, more containing barbaloin and aloe
Sugar.Aloe gel inspissated juice is preferably aloe vera gel inspissated juice.The type of essence is not particularly limited, preferably dendrobium nobile
Essence.
The preparation method of above-mentioned dendrobium candidum fermented beverage is described below comprising following steps: (1) premixization glue；(2)
Material allotment；(3) it sterilizes.
In step (1), xanthan gum, antierythrite and sodium citrate are premixed, rubber powder is obtained；Then rubber powder is slow
It puts into water, is stirred when putting into rubber powder；It is completely dissolved to rubber powder, no particle, glue is made, dendrobium candidum fermentation liquid is added,
It mixes, obtains the first mixed liquor.The temperature of water can be 60~80 DEG C, preferably 65~75 DEG C.It is stirred when putting into rubber powder
Time can be 10~30min, preferably 18~22min.The dosage of each component is as described above, which is not described herein again.
In step (2), essence, acesulfame potassium, aloe gel inspissated juice and Cordyceps militaris are added in the first mixed liquor, after
20~40min of continuous stirring, afterwards plus water constant volume obtains the second mixed liquor；Second mixed liquor is filtered, seasoning liquid is obtained.Continue
The time of stirring can be 20~40min, preferably 25~35min.Second mixed liquor is filtered wherein each to remove
The macroscopic solid particle of kind and other insoluble substance matter.The equipment of filtering is preferably the filtering of cloth bag duplex.The dosage of each component
As described above, which is not described herein again.
In step (3), seasoning liquid is de-gassed, homogeneous and sterilizing, obtains the dendrobium candidum fermented beverage.Sterilizing
Temperature can be 130~140 DEG C, preferably 132~137 DEG C.Sterilization time can be 4~10s.Sterilizing is preferably superhigh temperature wink
When sterilize.
A specific embodiment according to the present invention, the preparation method of dendrobium candidum fermented beverage include the following steps:
(1) premixization glue: xanthan gum, antierythrite and sodium citrate are premixed, rubber powder is obtained；Then rubber powder is slowly put into water
In, 10~30min is stirred when putting into rubber powder；It is completely dissolved to rubber powder, no particle, glue is made, dendrobium candidum fermentation is added
Liquid mixes, obtains the first mixed liquor；(2) material is deployed: essence, acesulfame potassium, aloe gel inspissated juice and Cordyceps militaris are added to
In first mixed liquor, continue 20~40min of stirring, water constant volume is added to obtain the second mixed liquor；Second mixed liquor is filtered, is obtained
To seasoning liquid；(3) it sterilizes: seasoning liquid is de-gassed and homogeneous, then sterilize at 130~140 DEG C 4~10s, obtains described
Dendrobium candidum fermented beverage.
The purposes > of < dendrobium candidum fermentation liquid and drink
Above-mentioned dendrobium candidum fermentation liquid of the invention has very significant nourishing Yin and clearing heat effect, and therefore, the present invention provides
Above-mentioned dendrobium candidum fermentation liquid has the health care product of nourishing Yin and clearing heat effect or the purposes of drink in preparation.Health care product or drink have
The meaning of this field routine, and and it is not belonging to position concept up and down.The nourishing Yin and clearing heat of dendrobium candidum fermentation liquid of the invention acts on master
Being embodied in improves hectic fever night sweat, constipation, insomnia.
Dendrobium candidum fermented beverage of the invention is used to prepare the purposes of health care product or drink with nourishing Yin and clearing heat effect.
Dendrobium candidum fermented beverage of the invention is liquid beverage, and rationally, multicomponent synergistic effect has good formula
Nourishing Yin and clearing heat effect, and it is in good taste.
Embodiment 1
<dendrobium candidum fermentation liquid>
By the dendrobium candidum fermentation liquid for 4000kg in terms of, dendrobium candidum fermentation liquid is made of the raw material of following component: iron
Skin dendrobium nobile fresh leaf 950kg, kiwi-fruit juice 420kg, sugarcane inspissated juice 250kg, white granulated sugar 120kg, wheat oligopeptide 9kg, dimension life
Plain C 9kg, sodium bicarbonate 13kg, Leuconostoc mesenteroides 0.22kg, Lactococcus lactis 0.22kg, cellulase 18kg, pectase
15kg, surplus are pure water.Wherein, effective bacterial content of Leuconostoc mesenteroides is 200,000,000,000 CFU/g；Lactococcus lactis it is effective
Bacterial content is 200,000,000,000 CFU/g；The enzyme activity of cellulase is 20,000 U/g；The enzyme activity of pectase is 30,000 U/g.
The preparation method of the dendrobium candidum fermentation liquid the following steps are included:
1) it is beaten: carrying out wet process disintegrator crushing and beating after 950kg dendrobium candidum fresh leaf is cleaned up, be beaten Shi Yaojia
Enter 9kg vitamin C to prevent brown stain from aoxidizing, obtains dendrobium candidum slurry.The mesh number of dendrobium candidum slurry is 90 mesh；
2) it digests: dendrobium candidum slurry being transferred in enzymatic vessel, 18kg cellulase and 15kg pectase is then added, is risen
Temperature digests 90min, obtains enzymolysis liquid to 50 DEG C.
3) mixingization sugar: after enzymatic hydrolysis by online batch mixer be added 120kg white granulated sugar, 9kg wheat oligopeptide,
420kg kiwi-fruit juice and 250kg sugarcane inspissated juice open stirring in charging, and 13kg sodium bicarbonate are added, by system pH tune
It is whole to 6.2, obtain mixed liquor.
4) it extracts sterilizing: mixed liquor being transferred in the empty fermentor to have disappeared in advance, 90 DEG C of heat preservation 30min are sterilized
Liquid.
5) it ferments: sterilized solution is cooled to 25 DEG C, be not required to dissolution zymophyte powder, zymophyte powder be directly added into, to access bacterium
Kind starts timing, controls 25 DEG C of temperature, and ferment 72h, obtains fermentation liquid；The zymophyte powder by 0.22kg Leuconostoc mesenteroides and
0.22kg Lactococcus lactis composition.
6) it filters: after fermentation, fermentation liquid being transferred in blend tank, by 40 mesh, 60 mesh cloth envelop collectors to hair
Zymotic fluid is filtered to obtain fermentation clear liquid, the as described dendrobium candidum fermentation liquid.
<dendrobium candidum fermented beverage>
By the dendrobium candidum fermented beverage for 50ml in terms of, dendrobium candidum fermented beverage is made of the raw material of following component:
Dendrobium candidum fermentation liquid 26g, Cordyceps militaris 0.7g, sodium citrate 0.22g, antierythrite 1.1g, acesulfame potassium 0.015g, xanthan gum
0.05g, dendrobium nobile essence 0.028g, aloe vera gel inspissated juice 0.7g, surplus is pure water.
The preparation method of the dendrobium candidum fermented beverage the following steps are included:
(1) premixization glue: 0.05g xanthan gum, 1.1g antierythrite, 0.22g sodium citrate are weighed and is premixed, glue is obtained
Powder；Then rubber powder is slowly put into 65 DEG C of water, 20min is stirred on the side Bian Tianjia；It is completely dissolved to rubber powder, glue is made in no particle
26g dendrobium candidum fermentation liquid is added in liquid, mixes, obtains the first mixed liquor；
(2) material deploy: by 0.028g dendrobium nobile essence, 0.015g acesulfame potassium, 0.7g aloe vera gel inspissated juice and
0.7g Cordyceps militaris is added in the first mixed liquor, continues to stir 30min, adds water to be settled to 50ml and obtain the second mixed liquor；To
Two mixed liquors carry out the filtering of cloth bag duplex, and wherein various macroscopic solid particles and other insoluble substance matter, filter bag are pressed for removing
16 mesh, 30 mesh, 40 mesh, 60 mesh, 80 mesh etc. are successively replaced, and seasoning liquid is obtained；
(3) it sterilizes: seasoning liquid being successively de-gassed, homogeneous, ultra high temperature short time sterilization, sterilising temp 132, when sterilizing
Between obtain dendrobium candidum fermented beverage for 4s.
Embodiment 2
Other than the raw material proportioning of dendrobium candidum fermented beverage, remaining condition is identical with embodiment 1:
By the dendrobium candidum fermented beverage for 50ml in terms of, dendrobium candidum fermented beverage is made of the raw material of following component:
Dendrobium candidum fermentation liquid 20g, Cordyceps militaris 0.8g, sodium citrate 0.3g, antierythrite 1.2g, acesulfame potassium 0.02g, xanthan gum
0.1g, dendrobium nobile essence 0.03g, aloe vera gel inspissated juice 0.8g, surplus is pure water.
Embodiment 3
Other than the raw material proportioning of dendrobium candidum fermented beverage, remaining condition is identical with embodiment 1:
By the dendrobium candidum fermented beverage for 50ml in terms of, dendrobium candidum fermented beverage is made of the raw material of following component:
Dendrobium candidum fermentation liquid 30g, Cordyceps militaris 0.6g, sodium citrate 0.3g, antierythrite 1.5g, acesulfame potassium 0.03g, xanthan gum
0.08g, dendrobium nobile essence 0.025g, aloe vera gel inspissated juice 0.6g, surplus is pure water.
Embodiment 4
Other than the raw material proportioning of dendrobium candidum fermentation liquid, remaining condition is identical with embodiment 1:
By the dendrobium candidum fermentation liquid for 4000kg in terms of, dendrobium candidum fermentation liquid is made of the raw material of following component: iron
Skin dendrobium nobile fresh leaf 1200kg, kiwi-fruit juice 550kg, sugarcane inspissated juice 300kg, white granulated sugar 200kg, wheat oligopeptide 12kg, dimension
Raw element C 15kg, sodium bicarbonate 18kg, Leuconostoc mesenteroides 0.3kg, Lactococcus lactis 0.3kg, cellulase 20kg, pectase
20kg, surplus are pure water.Wherein, effective bacterial content of Leuconostoc mesenteroides is 200,000,000,000 CFU/g；Lactococcus lactis it is effective
Bacterial content is 200,000,000,000 CFU/g；The enzyme activity of cellulase is 20,000 U/g；The enzyme activity of pectase is 30,000 U/g.
Embodiment 5
Other than the raw material proportioning of dendrobium candidum fermentation liquid, remaining condition is identical with embodiment 1:
By the dendrobium candidum fermentation liquid for 4000kg in terms of, dendrobium candidum fermentation liquid is made of the raw material of following component: iron
Skin dendrobium nobile fresh leaf 900kg, kiwi-fruit juice 450kg, sugarcane inspissated juice 400kg, white granulated sugar 80kg, wheat oligopeptide 10kg, dimension life
Plain C 10kg, sodium bicarbonate 10kg, Leuconostoc mesenteroides 0.15kg, Lactococcus lactis 0.25kg, cellulase 15kg, pectase
18kg, surplus are pure water.Wherein, effective bacterial content of Leuconostoc mesenteroides is 200,000,000,000 CFU/g；Lactococcus lactis it is effective
Bacterial content is 200,000,000,000 CFU/g；The enzyme activity of cellulase is 20,000 U/g；The enzyme activity of pectase is 30,000 U/g.
Embodiment 6
Other than the preparation method of dendrobium candidum fermentation liquid, remaining condition is identical with embodiment 1:
The preparation method of the dendrobium candidum fermentation liquid the following steps are included:
1) it is beaten: carrying out wet process disintegrator crushing and beating after 950kg dendrobium candidum fresh leaf is cleaned up, be beaten Shi Yaojia
Enter 9kg vitamin C to prevent brown stain from aoxidizing, obtains dendrobium candidum slurry.The mesh number of dendrobium candidum slurry is 90 mesh；
2) it digests: dendrobium candidum slurry being transferred in enzymatic vessel, 18kg cellulase and 15kg pectase is then added, is risen
Temperature digests 90min, obtains enzymolysis liquid to 50 DEG C.
3) mixingization sugar: be added by online batch mixer into enzymolysis liquid 120kg white granulated sugar, 9kg wheat oligopeptide,
420kg kiwi-fruit juice and 250kg sugarcane inspissated juice open stirring in charging, and 13kg sodium bicarbonate are added, by system pH tune
It is whole to 6.0, obtain mixed liquor.
4) it extracts sterilizing: mixed liquor being warming up to and is transferred to the empty fermentor to have disappeared in advance after 90 DEG C of instantaneous sterilizings (UHT)
In, cool down after 90 DEG C of heat preservation 30min, obtains sterilized solution.
5) it ferments: sterilized solution being cooled to 25 DEG C, zymophyte powder solution is added, start timing, control temperature to access strain
25 DEG C of degree, ferment 72h, obtains fermentation liquid；The zymophyte powder solution is by the zymophyte powder that weight ratio is 1:10 and water with obtained
It arrives；The zymophyte powder is made of 0.22kg Leuconostoc mesenteroides and 0.22kg Lactococcus lactis.
6) it filters: after fermentation, fermentation liquid being transferred in blend tank, by 40 mesh, 60 mesh cloth envelop collectors to hair
Zymotic fluid is filtered to obtain fermentation clear liquid, the as described dendrobium candidum fermentation liquid.
Experimental example 1
Fermentation liquid in the fermentation process of embodiment 1 is monitored, monitoring index includes viable count, total acid, pH value and thick
Polysaccharide, monitoring result is referring to Fig. 1~4.
<viable count>
It is measured with reference to GB 4789.35-2016.The viable count monitoring result of fermentation liquid in the fermentation process of embodiment 1
Referring to Fig. 1.
Lactic acid bacteria is the common name for the bacterium that a kind of fermentable sugars mainly generates a large amount of lactic acid, including lactobacillus, bifid bar
Pseudomonas etc..
Viable count monitoring method the following steps are included:
(1) sample preparation
Whole preparation process of sample follow sterile working program.With sterile suction after fermentation broth sample is first sufficiently shaken up
Pipe draws 25ml, is then placed in sterile conical flask (the preset an appropriate number of sterile glass in bottle equipped with 225ml physiological saline
Pearl) in, the even liquid of sample of 1:10 is made in shake well.
(2) lactic acid bacterial count
According to the estimation of the total viable count of fermentation broth sample, 2~3 continuous acceptable diluent degree are selected, each dilution is inhaled
Take the even liquid of 1ml fermentation broth sample in sterilizing plates, each dilution makees two plates.After dilution moves into plate, it will cool down
It is poured into plate about 15ml to 48 DEG C of MRS agar mediums, rotation plate makes to be uniformly mixed.36 DEG C of ± 1 DEG C of Anaerobic culturel 72h
±2h.Plate is diluted to from fermentation broth sample pours into requirement complete in 15min.
(3) calculation method
Choose plate count total plate count of the clump count between 30~300CFU, without sprawling bacterium colony growth.Lower than 30CFU
Plate record specific clump count, greater than can record can not mostly to count for 300CFU.The clump count of each degree should be flat using two
The average of plate.When one plate has the growth of larger sheet bacterium colony, then it should not use, and the plate of Ying Yiwu sheet bacterium colony growth
Clump count as the dilution；If sheet bacterium colony is less than the half of plate, and bacterium colony distribution is uniformly very in remaining half
It can.It calculates after half of plate multiplied by 2, represents a flat-plate bacterial colony number.Chain without obvious boundary line between occurring bacterium colony on plate
It is when growth, then single-stranded as a bacterium colony counting by every.
If the clump count on only one dilution plate is being suitable for calculating the flat of two flat-plate bacterial colony numbers in count range
Mean value, then by average value multiplied by corresponding extension rate, as total plate count result in every gram or every milliliter.
If there are two serial dilution degree culture dish clump count be suitable for count range in when, should be carried out by following formula based on
It calculates:
N=∑ C/ (nl+0.1n2) * d
Clump count in N-sample；
The sum of ∑ C-culture dish (culture dish of the clump count containing optimum range) clump count；
n1--- the first dilution (low extension rate) culture dish number；
n2--- the second dilution (highly diluted multiple) culture dish number；
D --- dilution gfactor (the first dilution).
If clump count is all larger than 300CFU on the plate of all dilutions, the highest plate of dilution is counted,
Other plates can record can not mostly to count, and as a result calculate by average colony number multiplied by highest extension rate.
If the flat-plate bacterial colony number of all dilutions is respectively less than 30CFU, should by the minimum average colony number of dilution multiplied by
Extension rate calculates.
If the sterile length of being born of all dilution (including fermentation broth sample stoste) plates, with small 1 multiplied by minimum dilution
Multiple calculates.
If the flat-plate bacterial colony number of all dilutions not between 30CFU~300CFU, a portion be less than 30CFU or
When big 300CFU, then calculated with the average colony number closest to 30CFU or 300CFU multiplied by extension rate.
(4) viable count monitoring result
As shown in Figure 1, probiotics is divided into three phases during the fermentation, probiotics is within the time of fermentation 3 days, viable bacteria
Number is slowly increased, and reaches 10 powers when fermenting the 1st day, until the 3rd day viable count is slowly increased, is maintained at the water of 10 powers
More than flat.
<total acidity test>
It is measured according to GB/T 12456-2008.The total acid monitoring result ginseng of fermentation liquid in the fermentation process of embodiment 1
See Fig. 2.
Titration end-point is determined by indicator of phenolphthalein with the acid in alkali-titration test solution using acid-base neutralization principle.By alkali
The consumption of liquid calculates total acid content.
As shown in Fig. 2, total acid content continues to increase in fermentation liquid in 3 days fermentation periods, from the initial 3g/L that ferments
Left and right increases to 10g/L.
<pHvalue determination>
Using the pH value of pH meter measurement fermentation liquid.In the fermentation process of embodiment 1 the pH value monitoring result of fermentation liquid referring to
Fig. 3.
As shown in figure 3, with the extension of fermentation time, fermentation liquid pH value elder generation dramatic decrease, rear slowly decline, downward trend
Slow down.PH value variation is divided into two stages: 1) fermentation starts to fermentation the 1st day, and probiotics breeding is very fast, and cell metabolism is extremely prosperous
It contains.This stage pH value declines rapidly, and reaches minimum value；PH value can reach 4.3~4.4 after this general stage.2) it ferments
2nd~3 day, growth of probiotics was more stable, and pH value decline is slower.
<Thick many candies measurement>
The Thick many candies monitoring result of fermentation liquid is referring to fig. 4 in the fermentation process of embodiment 1.
After carbohydrate can be dehydrated generation furfural or hydroxymethylfurfural by concentrated sulfuric acid effect at relatively high temperatures, with anthrone
(C14H10O) dehydrating condensation forms the derivative of furfural, is in blue-green.The substance has absorption maximum at 620nm, in certain model
In enclosing, the depth of color is directly proportional to soluble sugar content.
Thick many candies monitoring method the following steps are included:
(1) glucose standard: 100mg glucose (powder) is weighed in dissolution in small beaker and (is first dissolved in 50ml or so
Water in transfer Shi Yaoyong water repeatedly rinse), 100ml volumetric flask constant volume (concentration of standard solution 1mg/ml).
(2) concentrated sulfuric acid (specific gravity 1.84), anthrone are spare.
Anthrone concentrated sulfuric acid reagent: 0.2g anthrone is dissolved in the dense H of 100ml2SO4In, the same day, which prepares, uses (concentration 0.2%).
(3) make standard curve: take 6 25ml volumetric flasks, prepare a series of various concentrations (0,10 μ g/ml, 20 μ g/ml,
30 μ g/ml, 40 μ g/ml, 50 μ g/ml, 60 μ g/ml) glucose solution (titer).Take the above-mentioned each concentration standard liquid of 2.5ml
In test tube (blank adds 2.5ml distilled water), 6.5ml anthrone concentrated sulfuric acid reagent is added, mixes, is stored at room temperature one hour, Yu Bo
Colorimetric at long 620nm reads absorbance.Using absorbance as abscissa, concentration of standard solution is that ordinate does standard curve: Y=KX+
B(μg/ml)。
(4) Thick many candies measure
1. taking 10ml fermentation broth sample in 50ml centrifuge tube, 8500rpm is centrifuged 10min.
2. taking supernatant 5ml in 50ml centrifuge tube, 25ml dehydrated alcohol is added, capping topples over pipe for several times repeatedly.
3. setting 4 DEG C of refrigerator overnights.
4. being centrifuged 10min in centrifuge 5000rpm, supernatant is carefully toppled over.
5. 80% ethyl alcohol 10ml is added, mix, stand 10min, 5000rpm is centrifuged 10min, carefully topples over supernatant.
It is repeated once.
6. plus water dissolves by heating (ensuring whole dissolutions), is transferred to 50ml, 100ml, 250ml or 500ml volumetric flask in batches
And constant volume V1 (being determined according to sugared concentration).
7. taking 2.5ml prepare liquid in test tube, 0.2% anthrone concentrated sulfuric acid reagent 6.5ml is added, mixes, it is small to be stored at room temperature one
When, OD value is obtained at 620nm.
8. the calculation formula of Thick many candies content is as follows in fermentation broth sample:
X --- Thick many candies content [mg/ml] in fermentation broth sample；
Y --- the concentration (μ g/ml) of glucose in fermentation broth sample；
V1--- the constant volume total volume (ml) of fermentation broth sample；
1000 --- μ g/ml is converted to mg/ml；
5 --- the volume (ml) of fermentation broth sample；
0.9 --- glucose is scaled the coefficient of Thick many candies.
(5) Thick many candies monitoring result
As shown in figure 4, Thick many candies changes of contents is divided into two stages: 1) to the 2nd day or so, this stage was slightly more for fermentation starting
Sugared content is risen, but speedup is slower；Thick many candies content reaches 6~8g/L after this general stage.2) it ferments 2 days to 3
It, Thick many candies rise comparatively fast, and Thick many candies content reaches 12~14g/L after this general stage.
Experimental example 2
Experimental method: recruiting 30 with hectic fever, feverishness in palms and soles and with the volunteer of the symptoms such as constipation, insomnia, long acne,
Age is between 20~50 years old.Above-mentioned 30 volunteers take the dendrobium candidum fermented beverage of embodiment 1；Usage: every time 1
Bag, daily 1~2 time, per os hora somni is taken 10 days；It records mouthfeel and evaluates situation, and record efficacy assessments as a result, investigating emphatically
Improve night sweat, constipation, in terms of effect.
A. mouthfeel is evaluated
Mouthfeel evaluation criterion is as shown in table 1, and mouthfeel evaluation result is as shown in table 2.From table 1,2: for embodiment 1
The appearance luster scoring of dendrobium candidum fermented beverage, 30 volunteers is 23 points, bright in colour；Tissue morphology scoring is 22 points,
Form is uniform；Fragrance scoring is 24 points, and dendrobium nobile gives off a strong fragrance pure；Mouth feel score is 22 points, and delicate mouthfeel is pure.
1 mouthfeel evaluation criterion of table
The mouthfeel evaluation result of 2 dendrobium candidum fermented beverage of table
B. efficacy assessments
The effect of table 3 is the dendrobium candidum fermented beverage of embodiment 1 evaluation result.As shown in Table 3, hectic fever night sweat and constipation
The effect that symptom improves is most prominent, this is also the main performance of yin asthenia generating intrinsic heat；In addition, the improvement of insomnia is also very significant.This
Show that dendrobium candidum fermented beverage of the invention has good nourishing Yin and clearing heat effect.
The effect of 3 dendrobium candidum fermented beverage of table evaluation result
| Improvement situation | Improvement number | Ratio |
| Vexed insomnia improves | 5 | 16.7% |
| Constipation improves | 10 | 33.3% |
| Hectic fever night sweat improves | 12 | 40.0% |
| Long acne situation improves | 2 | 6.7% |
| Hair loss state improves | 1 | 3.3% |
Present invention is not limited to the embodiments described above, without departing from the essence of the present invention, this field skill
Any deformation, improvement, the replacement that art personnel are contemplated that each fall within the scope of the present invention.
Claims (10)
1. a kind of dendrobium candidum fermentation liquid, which is characterized in that it is by including that the raw material of following component is prepared: 400~1600
Parts by weight dendrobium candidum, 400~1200 parts by weight kiwi-fruit juices, 40~400 parts by weight sugarcane inspissated juices, 0.08~0.8 weight
Part zymophyte powder and 8~64 parts by weight enzymolysis agents.
2. dendrobium candidum fermentation liquid according to claim 1, which is characterized in that the zymophyte powder includes 0.04~0.4
Parts by weight Leuconostoc mesenteroides and 0.04~0.4 parts by weight Lactococcus lactis；And the enzymolysis agent includes 4~32 percent by weight fiber
Plain enzyme and 4~32 parts by weight pectases.
3. dendrobium candidum fermentation liquid according to claim 1, which is characterized in that the raw material further includes 40~400 weight
Part white granulated sugar, 4~20 parts by weight wheat oligopeptides, 4~20 part by weight of vitamin C and 4~40 parts by weight sodium bicarbonates.
4. dendrobium candidum fermentation liquid according to claim 1 or 3, which is characterized in that it is by the raw material including following component
Be prepared: 600~1400 parts by weight dendrobium candidums, 400~800 parts by weight kiwi-fruit juices, 100~400 parts by weight sugarcanes are dense
Contracting juice, 60~300 parts by weight white granulated sugars, 6~15 parts by weight wheat oligopeptides, 6~18 part by weight of vitamin C, 8~30 parts by weight
Sodium bicarbonate, 0.2~0.7 parts by weight zymophyte powder and 20~50 parts by weight enzymolysis agents.
5. the preparation method of dendrobium candidum fermentation liquid according to claim 3 or 4 comprising following steps:
1) it is beaten: dendrobium candidum is subjected to crushing and beating, obtain dendrobium candidum slurry；
2) it digests: enzymolysis agent is added in Xiang Suoshu dendrobium candidum slurry, is warming up to 40~60 DEG C, digests 60~120min, obtains enzyme
Solve liquid；
3) white granulated sugar, wheat oligopeptide, kiwi-fruit juice, sugarcane inspissated juice and carbonic acid mixingization sugar: are added in Xiang Suoshu enzymolysis liquid
Hydrogen sodium is uniformly mixed, and system pH is adjusted to 6.0~6.2, mixed liquor is obtained；
4) it sterilizes: the mixed liquor being kept the temperature into 30~90min under the conditions of temperature is 80~100 DEG C, obtains sterilized solution；
5) it ferments: the sterilized solution being cooled to 20~28 DEG C, zymophyte powder or zymophyte powder solution is added, ferment 60~96h,
Obtain fermentation liquid；The zymophyte powder solution is prepared to obtain by the zymophyte powder that weight ratio is 1:5~20 with water；
6) it filters: the fermentation liquid being filtered to obtain fermentation clear liquid, the as described dendrobium candidum fermentation liquid.
6. a kind of dendrobium candidum fermented beverage, which is characterized in that iron sheet stone including blender and as described in claim 3 or 4
The weight ratio of dry measure used in former times fermentation liquid, the dendrobium candidum fermentation liquid and the blender is 10~40:0.825~14.1.
7. dendrobium candidum fermented beverage according to claim 6, which is characterized in that the blender includes 0.1~2 weight
Part Cordyceps militaris, 0.1~0.5 parts by weight of lemon acid sodium, 0.5~3 parts by weight antierythrite, 0.005~0.1 parts by weight acesulfame potassium,
0.01~0.5 parts by weight xanthan gum, 0.01~0.1 parts by weight essence and 0.1~3 parts by weight aloe gel inspissated juice.
8. dendrobium candidum fermented beverage according to claim 7, which is characterized in that it is by the raw material system including following component
At: 15~35 parts by weight dendrobium candidum fermentation liquids, 0.3~1.5 parts by weight Cordyceps militaris, 0.15~0.4 parts by weight of lemon acid sodium,
0.6~2 parts by weight antierythrite, 0.008~0.08 parts by weight acesulfame potassium, 0.03~0.3 parts by weight xanthan gum, 0.015~
0.08 parts by weight essence and 0.2~2 parts by weight aloe gel inspissated juice.
9. the preparation method of dendrobium candidum fermented beverage according to claim 7 or 8 comprising following steps:
(1) premixization glue: xanthan gum, antierythrite and sodium citrate are premixed, rubber powder is obtained；In the rubber powder investment water,
10~30min is stirred, glue is obtained, dendrobium candidum fermentation liquid is added, mixes, obtains the first mixed liquor；
(2) material is deployed: essence, acesulfame potassium, aloe gel inspissated juice and Cordyceps militaris are added in first mixed liquor, after
20~40min of continuous stirring, adds water constant volume to obtain the second mixed liquor；Second mixed liquor is filtered, seasoning liquid is obtained；
(3) it sterilizes: the seasoning liquid being de-gassed, homogeneous and sterilizing, sterilising temp are 132~137 DEG C, sterilization time 4
~10s obtains the dendrobium candidum fermented beverage.
10. dendrobium candidum fermentation liquid according to any one of claims 1 to 4 is used to prepare the guarantor with nourishing Yin and clearing heat effect
The purposes of strong product or drink.
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