CN109198618A - Fermentation clear liquid that is antifatigue, improving sexual function and its preparation method and application - Google Patents
Fermentation clear liquid that is antifatigue, improving sexual function and its preparation method and application Download PDFInfo
- Publication number
- CN109198618A CN109198618A CN201811072802.8A CN201811072802A CN109198618A CN 109198618 A CN109198618 A CN 109198618A CN 201811072802 A CN201811072802 A CN 201811072802A CN 109198618 A CN109198618 A CN 109198618A
- Authority
- CN
- China
- Prior art keywords
- weight
- parts
- fermentation
- clear liquid
- obtains
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention discloses a kind of with antifatigue and improvement sexual function effect fermentation clear liquid and its preparation method and application.The fermentation clear liquid is by including that the raw material of following component is prepared: 500~550 parts by weight of leek seedling, 100~140 parts by weight of rhizoma polygonati, 60~100 parts by weight of raspberry, 60~100 parts by weight of Chinese yam, 60~100 parts by weight of Semen sesami nigrum, 600~1000 parts by weight of fruit, vegetable juice, 0.4~1.4 parts by weight of leavening, 2200~2700 parts by weight of 9~30 parts by weight of enzyme preparation and water.Fermentation clear liquid of the invention has good antifatigue, improvement sexual function effect, and is easy to absorb, and raw material is cheap and easy to get.
Description
Technical field
The present invention relates to a kind of with fermentation clear liquid that is antifatigue, improving sexual function, further relates to the preparation of the fermentation clear liquid
Method and purposes.
Background technique
Fatigue is a kind of physiological phenomenon of complexity, body under certain condition, due to for a long time or overwrought physical strength
Or labor efficiency caused by mental activity tends to decline, can lead to working efficiency reduction.Fatiguability is the one of sub-health state
Kind performance gradually accumulates if cannot restore in time after fatigue generation, also results in overwork, makes body that endocrine occur disorderly
Disorderly, immunity degradation.Over fatigue can also result in sex dysfunction, especially the sexual function erectile dysfunction of influence male, ejaculation
The illness rates such as obstacle, seriously affect life quality.
Have at present with multiple product and technology antifatigue, that improve sexual function effect.CN201711216015.1 is public
Opened a kind of improvement male's sexual, antifatigue composition, the raw material including following parts by weight: 5~30 parts of oyster peptide, cream are more
1~10 part of peptide, 1~10 part of ginseng pulverate, 1~10 part of pueraria root powder, 0~15 part of black ginger powder, 1~10 part of burdock root powder, mulberries powder 5~
15 parts, 5~15 parts of Fructus Schisandrae Chinensis powder, 5~15 parts of medlar powder, 1~10 part of dark plum powder, 10~50 parts of isomaltoketose, trichlorine sugarcane
0.1~0.5 part of sugar.Preparation method is that each component directly mixes.The composition is mostly that medicinal powder is directly used as medicine, and influences to absorb effect
Fruit.
CN201210386020.8 disclose it is a kind of raising immunity, it is antifatigue, improve sexual function Chinese medicine composition,
Raw material including following parts by weight: 25~35 parts of maca, 1~6 part of Shorthorned Epimedium P.E, 5~15 parts of gadol extract, meat desert
1~10 part of Rong's extract, 5~15 parts of siberian Ginseng P.E, 5~20 parts of pallas pit viper powder, preparation method is that maca, pallas pit viper are through powder
Obtained powder after broken；Root of kirilow rhodiola, wilsonii, which are adopted, to be extracted with water to obtain water extract；Herba Cistanches, Herba Epimedii obtain alcohol using alcohol extracting
Extract, above-mentioned powder, water extract, alcohol extract are mixed to get the Chinese medicine composition.The composition has used a variety of rare medicinal materials
Or rare medicinal herbs, it is expensive；And preparation method is conventional method.
Therefore, there is still a need for a kind of with definite reliable product that is antifatigue, improving sexual function.
Summary of the invention
In view of this, the first purpose of this invention is to provide a kind of fermentation clear liquid, with good antifatigue and
Improve sexual function effect, is easy to absorb, and raw material is cheap and easy to get.
Second object of the present invention is to provide the preparation method of above-mentioned fermentation clear liquid, and fermentation period is shorter, is conducive to
Large-scale production.
Third object of the present invention is to provide a kind of fermented beverage, with good antifatigue and improvement sexual function
Effect, and appearance is vivid, mouthfeel and delicious, is easy to receive.
Fourth object of the present invention is to provide the purposes of above-mentioned fermentation clear liquid.
The present invention provide it is a kind of with antifatigue and improve the fermentation clear liquid of sexual function effect, by including following component
Raw material is prepared: 500~550 parts by weight of leek seedling, 100~140 parts by weight of rhizoma polygonati, 60~100 parts by weight of raspberry, Chinese yam
60~100 parts by weight, 60~100 parts by weight of Semen sesami nigrum, 600~1000 parts by weight of fruit, vegetable juice, 0.4~1.4 parts by weight of leavening,
2200~2700 parts by weight of 9~30 parts by weight of enzyme preparation and water.
Fermentation clear liquid according to the present invention, it is preferable that the raw material also comprises the following components: 10~20 weight of Cordyceps militaris
Part, 50~100 parts by weight of Herba Epimedii, 50~100 parts by weight of 50~100 parts by weight of Schisandra chinensis and Morinda officinalis.
Fermentation clear liquid according to the present invention, it is preferable that the fruit, vegetable juice is will to obtain after following fruits and vegetables crushing and beating
It arrives: 150~240 parts by weight of apple, 150~240 parts by weight of pears, 100~210 parts by weight of peach, 50~130 parts by weight of banana, Hu
20~60 parts by weight of radish, 20~45 parts by weight of 30~70 parts by weight of tomato, 20~45 parts by weight of cucurbita pepo and broccoli.
Fermentation clear liquid according to the present invention, it is preferable that the enzyme preparation includes 6~10 parts by weight of cellulase, pectase 6
6~10 parts by weight of~10 parts by weight and acid protease；The leavening includes 0.2~0.7 parts by weight of Lactobacillus casei, plant
0.1~0.35 parts by weight of 0.1~0.35 parts by weight of lactobacillus and bifidobacterium adolescentis.
Fermentation clear liquid according to the present invention, it is preferable that the raw material further includes nitrogen source, and the dosage of the nitrogen source be 30~
60 parts by weight；The nitrogen source is selected from one or more of wheat oligopeptide, Soyprotein peptide and yeast extract.
The present invention also provides the preparation methods of the fermentation clear liquid, include the following steps:
(1) leek seedling, rhizoma polygonati, Semen sesami nigrum, raspberry, Chinese yam, fruit, vegetable juice and water are mixed to form mixture, by institute
It states mixture to be digested under the action of enzyme preparation, obtains enzymolysis liquid；Hydrolysis temperature be 40~60 DEG C, enzymolysis time be 60~
180min；Or
By the leek seedling, rhizoma polygonati, Semen sesami nigrum, raspberry, Chinese yam, Cordyceps militaris, Herba Epimedii, Schisandra chinensis, Morinda officinalis, fruits and vegetables
Slurry and water are mixed to form mixture, and the mixture is digested under the action of enzyme preparation, obtains enzymolysis liquid；Hydrolysis temperature
It is 40~60 DEG C, enzymolysis time is 60~180min；
(2) enzymolysis liquid is subjected to enzyme deactivation extraction process, obtains enzyme deactivation extracting solution；Enzyme deactivation Extracting temperature is 80~100
DEG C, enzyme deactivation extraction time is 0.4~1.0h；
(3) it by the enzyme deactivation extracting solution, is optionally uniformly mixed with the nitrogen source, obtains mixture；By the mixing
Object adjusts pH value to 5~7, and sterilization treatment obtains sterilizing product；The sterilizing product is subjected to fermentation process through the leavening,
Obtain tunning；The tunning is separated by solid-liquid separation, the fermentation clear liquid is obtained.
Preparation method according to the present invention, it is preferable that step (3) described fermentation process are as follows: first connect Lactobacillus casei
Kind to the sterilizing product, ferment 12~16d in the case where temperature is 25~40 DEG C and mixing speed is 20~100r/min, obtains the
One fermentation liquid；Lactobacillus plantarum and bifidobacterium adolescentis are seeded to first fermentation liquid, is 25~40 DEG C in temperature and stirs
Mixing speed is the 4~8d that ferments under 20~100r/min, obtains the second fermentation liquid as the tunning.
The present invention also provides a kind of with antifatigue and improvement sexual function effect fermented beverage comprising the fermentation is clear
Liquid, blender and water, the blender include crystal diabetin, inulin, antierythrite, cyclohexaamylose, thickener, sucrose fat
Acid esters, zinc gluconate, xylo-oligosaccharide, caramel syrup, inspissated juice, essence, sweetener, trehalose, sodium citrate and Guarana
Extract.
The present invention also provides the preparation methods of the fermented beverage, include the following steps:
A. by the crystal diabetin, inulin, antierythrite, cyclohexaamylose and thickener are uniformly mixed and obtain premix；
B. the premix is slowly added in the water of part, water temperature is 60~80 DEG C, carries out first time stirring；Then it is added
Sucrose fatty ester carries out second and stirs, obtains mixed liquor；
C. by the fermentation clear liquid, zinc gluconate, xylo-oligosaccharide, caramel syrup, inspissated juice, essence, sweetener, seaweed
Sugar, sodium citrate, Guarana extract and remaining water are added in the mixed liquor, carry out third time stirring, filter, sterilizing,
Obtain the fermented beverage.
The present invention also provides the fermentation clear liquid be used to prepare with it is antifatigue and/or improve sexual function effect drug,
The purposes of food or drink.
Based on fermentation clear liquid of the invention selects medicinal raw material leek seedling, rhizoma polygonati, raspberry, Chinese yam and Semen sesami nigrum, adopt
It is substrate with fruit, vegetable juice, it is ferment-fermented, and reasonable disposition each component content, it cooperates between each raw material, acts synergistically, obtain
The fermentation clear liquid arrived have it is good it is antifatigue, improve sexual function effect, be easy to absorb, and medicinal raw material is cheap is easy to get.
Further, the medicinal raw material further includes Cordyceps militaris, Herba Epimedii, Schisandra chinensis, Morinda officinalis, and the fermentation clear liquid being prepared has
Preferably antifatigue, improvement sexual function effect.The present invention keeps reaction substrate full of nutrition, leads to by the way that preferred fruit, vegetable juice is added
After crossing enzymatic hydrolysis and fermentation process, it is more advantageous to being absorbed and utilized for nutritional ingredient.It is fermented using currently preferred leavening,
It is more suitable for fermentation substrate of the invention, higher viable count can be kept for a long time during the fermentation, decline speed is slow, has
Good metabolic activity, and the fermentation clear liquid obtained has better taste and flavor.Using currently preferred nitrogen source type
And dosage, fermentation processes and enzyme amount expression to currently preferred leavening have significant positive influences, are able to suppress
The growth of miscellaneous bacteria guarantees that the quality of fermentation material is stablized.Preparation method simple process of the invention, the period is shorter, is suitble to industrialization
Production.The medicinal raw material of fermentation clear liquid of the invention is dual-purpose of drug and food, takes safety, without side-effects.
Detailed description of the invention
Fig. 1 is the fermentation clear liquid viable count trend chart during the fermentation of embodiment 1.
Fig. 2 is the fermentation clear liquid total acid trend chart during the fermentation of embodiment 1.
Fig. 3 is the fermentation clear liquid pH trend chart during the fermentation of embodiment 1.
Fig. 4 is the fermentation clear liquid total sugar content trend chart during the fermentation of embodiment 1.
Specific embodiment
The present invention is further illustrated combined with specific embodiments below, but protection scope of the present invention is not limited to
This.
The dosage " part " of the present invention, component indicate parts by weight.
In the present invention, leek seedling is the tender of liliaceous plant leek Allium tuberosum Rottl.ex Spreng.
Seedling.Rhizoma polygonati is liliaceous plant polygonatum kingianurn Polygonatum kingianum Coll.et Hemsl., rhizoma polygonati Polygonatum
The dry rhizome of sibiricum Red. or polygonatum cyrtonema Polygonatum cyrtonema Hua.Semen sesami nigrum is Pedaliaceae plant
The dry mature seed of object sesame Sesamum indicum L..Raspberry is rosaceous plant rubus chingii Hu Rubus
The dry fruit of chingii Hu.Chinese yam is the dry rhizome of Dioscoreaceae plant Chinese yam Dioscorea opposite Thumb..
Cordyceps militaris is Clavicipitaceae Cordyceps Cordyceps militaris Cordyceps militaris (Linn.et Fr.) Link.Herba Epimedii is barberry
Section plant Herba Epimedii Epimedium brevicomu Maxim., Epimedium sagittatum Epimedium sagittatum (Sieb.et
Zucc.) Maxim., E. Pubescens Epimedium pubescens Maxim. or korean epimedium herb Epimedium
The dried leaf of koreanum Nakai.Schisandra chinensis is magnoliaceae schisandra Schisandra chinensis (Turcz.)
Baill. dry mature fruit.Morinda officinalis is the dry root of madder wort Morinda officinalis Morinda officinalis How.
In the present invention, wheat oligopeptide is using wheat gluten flour as raw material, sized mixing, protease hydrolyzed, separation, filtering,
Micromolecule polypeptide substance made of the techniques such as spray drying.
Soyprotein peptide is soybean protein after albumen enzyme effect, then is separated, refined the protein hydrolysis production of acquisition
Object is collectively constituted by a variety of peptide mixers, and peptide molecular weight is below 10000.
Yeast extract is using food yeast as primary raw material, with the collective effect of the enzyme of yeast itself or external enzyme
Under, the product that is obtained after enzymolysis.
Guarana extract refers to from the fragrant cocoa Paullinia of Sapindaceae rattan shape shrub Brazil for originating in Brazil
The solid powder extracted in cupana Kunth seed, wherein the content of caffeine is in 10~25wt%.It can directly adopt commercially available
Product.
<fermentation clear liquid>
Fermentation clear liquid of the invention has antifatigue and improves sexual function effect, is prepared by the raw material for including following component
It obtains: 500~550 parts by weight of leek seedling, 100~140 parts by weight of rhizoma polygonati, 60~100 parts by weight of raspberry, Chinese yam 60~100
Parts by weight, 60~100 parts by weight of Semen sesami nigrum, 600~1000 parts by weight of fruit, vegetable juice, 0.4~1.4 parts by weight of leavening, enzyme preparation 9
2200~2700 parts by weight of~30 parts by weight and water.According to embodiment of the present invention, the work of fermentation clear liquid of the invention
Property ingredient is only prepared by above-mentioned raw materials.
In the present invention, it is preferable that fermentation clear liquid is by including that the following group of raw material being grouped as is prepared: leek seedling 510~
530 parts by weight, 110~125 parts by weight of rhizoma polygonati, 70~90 parts by weight of raspberry, 80~95 parts by weight of Chinese yam, Semen sesami nigrum 80~95
Parts by weight, 700~950 parts by weight of fruit, vegetable juice, 0.5~1.2 parts by weight of leavening, 12~30 parts by weight of enzyme preparation and water 2200~
2500 parts by weight.According to embodiment of the present invention, fermentation clear liquid of the invention is only prepared by above-mentioned raw materials.
In the present invention, it is highly preferred that fermentation clear liquid is by including that the following group of raw material being grouped as is prepared: leek seedling 515
Parts by weight, 118 parts by weight of rhizoma polygonati, 75 parts by weight of raspberry, 90 parts by weight of Chinese yam, 90 parts by weight of Semen sesami nigrum, 800 weight of fruit, vegetable juice
Part, 0.8 parts by weight of leavening, 2300 parts by weight of 24 parts by weight of enzyme preparation and water.According to embodiment of the present invention, this hair
Bright fermentation clear liquid is only prepared by above-mentioned raw materials.
In the present invention, it is preferable that fermentation clear liquid is by including that the following group of raw material being grouped as is prepared: leek seedling 500~
550 parts by weight, 100~140 parts by weight of rhizoma polygonati, 60~100 parts by weight of raspberry, 60~100 parts by weight of Chinese yam, Semen sesami nigrum 60~
100 parts by weight, 10~20 parts by weight of Cordyceps militaris, 50~100 parts by weight of Herba Epimedii, 50~100 parts by weight of Schisandra chinensis, Morinda officinalis 50
~100 parts by weight, 600~1000 parts by weight of fruit, vegetable juice, 0.4~1.4 parts by weight of leavening, 9~30 parts by weight of enzyme preparation and water
2200~2700 parts by weight.According to embodiment of the present invention, fermentation clear liquid of the invention is only prepared by above-mentioned raw materials
It arrives.
In the present invention, it is preferable that fermentation clear liquid is by including that the following group of raw material being grouped as is prepared: leek seedling 510~
530 parts by weight, 110~125 parts by weight of rhizoma polygonati, 70~90 parts by weight of raspberry, 80~95 parts by weight of Chinese yam, Semen sesami nigrum 80~95
Parts by weight, 10~15 parts by weight of Cordyceps militaris, 60~80 parts by weight of Herba Epimedii, 60~80 parts by weight of Schisandra chinensis, Morinda officinalis 60~80
700~950 parts by weight of parts by weight fruit, vegetable juice, 0.5~1.2 parts by weight of leavening, 12~30 parts by weight of enzyme preparation and water 2200~
2500 parts.According to embodiment of the present invention, fermentation clear liquid of the invention is only prepared by above-mentioned raw materials.
Based on the present invention selects raw material leek seedling, rhizoma polygonati, raspberry, Chinese yam and the Semen sesami nigrum of dual-purpose of drug and food, fruit, vegetable juice
For substrate, post-fermentation, and reasonable disposition each component content are digested, cooperates, acts synergistically between each raw material, obtained fermentation
Liquid has good antifatigue, improvement sexual function effect, and takes safety, without side-effects.By further adding dual-purpose of drug and food
Cordyceps militaris, Herba Epimedii, Schisandra chinensis based on Morinda officinalis, cooperates fruit, vegetable juice, and enzymatic hydrolysis post-fermentation can further improve fermentation liquid
It is antifatigue, improve sexual function effect.
In the present invention, the leek seedling and rhizoma polygonati can feed intake in form of slurry respectively plus after water crushing and beating, or
It feeds intake in form of slurry after person's leek seedling and rhizoma polygonati can mix plus after water crushing and beating.The raspberry, Cordyceps militaris, excessive sheep
The leaves of pulse plants, Schisandra chinensis, Morinda officinalis and Chinese yam can feed intake in the form of a powder.Preferably, the raspberry, Cordyceps militaris, Herba Epimedii, five
The partial size of taste, Morinda officinalis and Chinese yam is respectively 50~150 microns, and preferably 60~120 microns, more preferably 70~100 is micro-
Rice.Semen sesami nigrum can feed intake in powder form, after can also being mixed with leek seedling, rhizoma polygonati plus water crushing and beating after as a slurry
It feeds intake.
In the present invention, the fruit, vegetable juice is will to obtain after fresh fruit and vegetables crushing and beating.The fruit, vegetable juice
Fineness can be 40~80 mesh, to be conducive to the abundant effect of strain and nutritional substrate in subsequent fermentation program.The fruit
And/or vegetables can be seasonal fruits and vegetables.The fruit can be selected from apple, pears, peach, banana, shaddock, lemon, orange, grape
One or more of.The vegetables can selected from one of carrot, tomato, cucurbita pepo, broccoli, cucumber, lotus rhizome or
It is several.A preferred embodiment according to the present invention, the fruit, vegetable juice are will to obtain after following fruits and vegetables crushing and beating
It arrives: 150~240 parts by weight of apple, 150~240 parts by weight of pears, 100~210 parts by weight of peach, 50~130 parts by weight of banana, Hu
20~60 parts by weight of radish, 20~45 parts by weight of 30~70 parts by weight of tomato, 20~45 parts by weight of cucurbita pepo and broccoli.It is excellent
Selection of land, the fruit, vegetable juice are will to obtain after following fruits and vegetables crushing and beating: 180~210 parts by weight of apple, pears 180~210
Parts by weight, 120~160 parts by weight of peach, 70~110 parts by weight of banana, 30~50 parts by weight of carrot, 40~60 weight of tomato
Part, 30~45 parts by weight of 30~45 parts by weight of cucurbita pepo and broccoli.According to embodiment of the present invention, the fruit, vegetable juice
It is that will be obtained after following fruits and vegetables crushing and beating: 190 parts by weight of apple, 180 parts by weight of pears, 160 parts by weight of peach, banana 90
Parts by weight, 50 parts by weight of carrot, 40 parts by weight of 60 parts by weight of tomato, 40 parts by weight of cucurbita pepo and broccoli.
The present invention keeps reaction substrate full of nutrition, after enzymatic hydrolysis and fermentation process, is more advantageous to by the way that fruit, vegetable juice is added
Nutritional ingredient is absorbed and utilized.In addition, fruit, vegetable juice of the invention avoids fermentation process as the carbon source in subsequent fermentations
In additionally add the carbon sources such as sucrose.
Enzyme preparation of the invention is used to digest fruit, vegetable juice and other each raw materials, decomposes in pectin and degradation cell wall
Cellulose so that the nutritional ingredient in traditional Chinese medicinal material raw materials more fully releases；It is also beneficial to simultaneously by Chinese medicine
Breaks down proteins in material raw material are more advantageous to absorption of human body at the smaller small peptide of molecular weight；In addition, making the flowing of material system
Property it is more preferable, be conducive to mass transfer in fermentation process, heat transfer, fermentation process is more stable.In the present invention, the enzyme preparation may include
6~10 parts by weight of 6~10 parts by weight of cellulase, 6~10 parts by weight of pectase and acid protease；Preferably include cellulase
7~9 parts by weight of 7~10 parts by weight, 7~10 parts by weight of pectase and acid protease.The enzyme activity of cellulase can for 1~
30000 U/g, preferably 2~30,000 U/g.The enzyme activity of pectase is 1~30,000 U/g, preferably 2~30,000 U/g.Acid protease
Enzyme activity can be 6~120,000 U/g, preferably 8~100,000 U/g.According to a preferred embodiment of the present invention, the enzyme system
Agent is made of 6~10 parts by weight of cellulase, 6~10 parts by weight of pectase and 6~10 parts by weight of acid protease；It is preferred that by fibre
Tie up 7~10 parts by weight of plain enzyme, 7~9 parts by weight of 7~10 parts by weight of pectase and acid protease.
Leavening of the invention can be selected from the one or more of lactic acid bacteria, yeast, monascus or bacillus subtilis.Institute
Lactobacillus casei, bifidobacterium adolescentis, Lactobacillus rhamnosus, lactobacillus plantarum, Leuconostoc mesenteroides can be selected from by stating lactic acid bacteria
It is one or more with bifidobacterium breve.The yeast is selected from one or more of saccharomyces cerevisiae, Kluyveromyces lactis.
The dosage of leavening can be 0.4~1.4 parts by weight, preferably 0.5~1.2 parts by weight.
In the present invention, it is preferable that the leavening includes 0.2~0.7 parts by weight of Lactobacillus casei, lactobacillus plantarum 0.1
0.1~0.35 parts by weight of~0.35 parts by weight and bifidobacterium adolescentis；It preferably includes 0.3~0.5 parts by weight of Lactobacillus casei, plant
0.15~0.25 parts by weight of 0.15~0.25 parts by weight of object lactobacillus and bifidobacterium adolescentis.One according to the present invention preferred
Embodiment, the leavening are only made of the Lactobacillus casei, lactobacillus plantarum and bifidobacterium adolescentis of above-mentioned parts by weight.It is dry
Lactobacillus paracasei, lactobacillus plantarum and bifidobacterium adolescentis can be using those of known in the art.In the present invention, Lactobacillus casei
Effective bacterial content can be 2000~500,000,000,000 CFU/g, preferably 2500~400,000,000,000 CFU/g.Lactobacillus plantarum it is effective
Bacterial content can be 1500~400,000,000,000 CFU/g, preferably 2000~350,000,000,000 CFU/g.Effective bacterium of bifidobacterium adolescentis contains
Amount can be 1500~400,000,000,000 CFU/g, preferably 2000~350,000,000,000 CFU/g.
For fermentation substrate of the invention, Lactobacillus casei, lactobacillus plantarum and bifidobacterium adolescentis are especially excellent
The leavening of choosing has both multiple advantage.For fermentation substrate of the invention, using Lactobacillus casei, lactobacillus plantarum and blueness
When these three strains of spring Bifidobacterium are as leavening, the fermentation clear liquid of acquisition has preferably antifatigue, improvement sexual function work
With；Meanwhile compared with other kinds of leavening, above-mentioned preferred leavening can keep for a long time higher during the fermentation
Viable count, decline speed it is slow, have good metabolic activity.In addition, above-mentioned preferred leavening is to fermentation substrate of the present invention
After being fermented, the fermentation clear liquid of acquisition is substantially better than other strains and combination in terms of mouthfeel, flavor.
Raw material of the invention can also include nitrogen source.The nitrogen source is preferably organic nitrogen source, more preferably wheat oligopeptide,
One or more of Soyprotein peptide and yeast extract.According to embodiment of the present invention, the nitrogen source is weight
Than for the wheat oligopeptide of 1:1 and the mixture of Soyprotein peptide.According to another implementation of the invention, the nitrogen source is
Wheat oligopeptide.The dosage of the nitrogen source can be 30~60 parts by weight, preferably 35~50 parts by weight.It is above-mentioned preferred organic
The type and dosage of nitrogen source, fermentation processes and enzyme amount expression to currently preferred leavening have significant positive face
It rings, is able to suppress the growth of miscellaneous bacteria, guarantee that the quality of fermentation material is stablized.
<preparation method>
The preparation method of above-mentioned fermentation liquid is described below comprising (1) enzymolysis step, (2) enzyme deactivation step and (3) fermentation step
Suddenly.
In step (1), when medicinal raw material is only made of leek seedling, rhizoma polygonati, Semen sesami nigrum, raspberry and Chinese yam, by institute
Leek seedling, rhizoma polygonati, Semen sesami nigrum, raspberry, Chinese yam, fruit, vegetable juice and the water stated are mixed to form mixture, by the mixture in enzyme
It is digested to obtain enzymolysis liquid under the action of preparation.Hydrolysis temperature can be 40~60 DEG C, preferably 45~55 DEG C.Enzymolysis time
It can be 60~180min, preferably 90~120min.
In step (1), when medicinal raw material by leek seedling, rhizoma polygonati, Semen sesami nigrum, raspberry, Chinese yam, Cordyceps militaris, Herba Epimedii,
When Schisandra chinensis and Morinda officinalis form, by the leek seedling, rhizoma polygonati, Semen sesami nigrum, raspberry, Chinese yam, Cordyceps militaris, Herba Epimedii, five
Taste, Morinda officinalis, fruit, vegetable juice and water are mixed to form mixture, and the mixture is digested to obtain under the action of enzyme preparation
Enzymolysis liquid.Hydrolysis temperature can be 40~60 DEG C, preferably 45~55 DEG C.Enzymolysis time can be 60~180min, preferably
90~120min.
In step (1), it is preferable that leek seedling and rhizoma polygonati can be thrown in form of slurry respectively plus after water crushing and beating
It feeds intake in form of slurry after material or leek seedling and rhizoma polygonati can mix plus after water crushing and beating.The raspberry, pupa worm
Grass, Herba Epimedii, Schisandra chinensis, Morinda officinalis and Chinese yam can feed intake in the form of a powder.Preferably, the raspberry, Cordyceps militaris are excessive
The partial size of the sheep leaves of pulse plants, Schisandra chinensis, Morinda officinalis and Chinese yam is respectively 50~150 microns, preferably 60~120 microns, more preferably 70
~100 microns.Semen sesami nigrum can feed intake in powder form, and water crushing and beating is added after can also mixing with above-mentioned leek seedling, rhizoma polygonati
It feeds intake as a slurry afterwards.The dosage of leek seedling can be 500~550 parts by weight, preferably 510~530 parts by weight.Rhizoma polygonati
Dosage can be 100~140 parts by weight, preferably 110~125 parts by weight.The dosage of raspberry can be 60~100 weight
Part, preferably 70~90 parts by weight.The dosage of Chinese yam can be 60~100 parts by weight, preferably 80~95 parts by weight.Semen sesami nigrum
Dosage can for 60~100 parts by weight, preferably 80~95 parts by weight.
In the present invention, the fruit, vegetable juice is will to obtain after fresh fruit and vegetables crushing and beating, the fruits and vegetables
Type as it was noted above, repeating no more.According to a preferred embodiment, the fruit, vegetable juice by apple, pears, peach, banana,
Carrot, tomato, cucurbita pepo and broccoli crushing and beating are made.Crushing and beating can be carried out using beater.In pulping process
In, antioxidant, such as vitamin C can be added, to prevent fruits and vegetables during crushing and beating from aoxidizing, ascorbic dosage
It can be 2~8 parts by weight, preferably 3~6 parts by weight.It is mixed after every kind of water fruits and vegetables being distinguished crushing and beating, it can also
With crushing and beating again after mixing every kind of water fruits and vegetables.In the fruit, vegetable juice, the dosage of various water fruits and vegetables is as described above,
It repeats no more.
In step (1), the dosage of water can be 2200~2700 parts by weight, preferably 2200~2500 parts by weight.
In step (1), enzymatic hydrolysis carries out in the presence of enzyme preparation.The enzyme preparation is as described above, repeats no more.Step
(1) in, hydrolysis temperature can be 40~60 DEG C, preferably 45~55 DEG C.Enzymolysis time can be 60~180min, preferably 80
~150min.
A specific embodiment according to the present invention, in step (1), by 515 parts by weight of leek seedling, 118 weight of rhizoma polygonati
Mashing obtains mixed liquor after part and 90 parts by weight rehydration of Semen sesami nigrum；75 parts by weight of raspberry, 90 parts by weight of Chinese yam are crushed respectively
It is 70~100 microns to partial size, respectively obtains raspberry powder and yam flour；By fresh 190 parts by weight of apple, 180 weight of pears
Part, 160 parts by weight of peach, 90 parts by weight of banana, 50 parts by weight of carrot, 60 parts by weight of tomato, 40 parts by weight of cucurbita pepo, west are blue
It spends 40 parts by weight and 6 parts by weight of vitamin C to carry out crushing and beating, obtains fruit, vegetable juice；By the mixed liquor, raspberry powder, Chinese yam
Powder, fruit, vegetable juice and water are uniformly mixed, and 8 parts by weight of cellulase, 8 parts by weight of 8 parts by weight of pectase and acid protease are added, in
90min is digested at 55 DEG C, obtains enzymolysis liquid.
In step (2), the enzymolysis liquid is subjected to enzyme deactivation extraction process, obtains enzyme deactivation extracting solution.Enzyme deactivation Extracting temperature
It can be 80~100 DEG C, preferably 90~95 DEG C.Enzyme deactivation extraction time can be 0.4~1.0h, preferably 0.5~0.6h.
In step (3), the enzyme deactivation extracting solution is optionally uniformly mixed with nitrogen source, obtains mixture, adjusts pH
For value to 5~7, sterilization treatment obtains sterilizing product；Fermented dose of progress fermentation process of the sterilizing product is obtained into tunning；
The tunning is separated by solid-liquid separation, the fermentation clear liquid is obtained.The type and dosage of the nitrogen source are as described above, no
It repeats again.
In step (3), the pH value of mixture is adjusted to 5~7, preferably 5.5~6.5.Using above-mentioned pH value range, favorably
The proliferation of strain during fermented mixture.In the present invention, the pH adjusting agent for adjusting pH value is preferably dipotassium hydrogen phosphate
With the mixture or sodium bicarbonate of potassium dihydrogen phosphate.Preferably, the pH adjusting agent is sodium bicarbonate.
In step (3), the sterilising temp of the sterilization treatment can be 100~155 DEG C, preferably 120~150 DEG C.It goes out
The bacterium time can be 3~15s, preferably 5~10s.
In step (3), the dosage of leavening can be 0.4~1.4 parts by weight, preferably 0.5~1.2 parts by weight.Institute
It is as described above to state leavening, repeats no more.
In the present invention, it is preferable that step (3) fermentation process is the fermentation at least two stages, and fermentation condition is
It is different.According to embodiment of the present invention, 0.2~0.7 parts by weight of Lactobacillus casei the sterilizing is seeded to first to produce
Object, ferment 12~16d in the case where temperature is 25~40 DEG C and mixing speed is 20~100r/min, obtains the first fermentation liquid；It will plant
0.1~0.35 parts by weight of object lactobacillus and 0.1~0.35 parts by weight of bifidobacterium adolescentis are seeded to first fermentation liquid, in temperature
Degree is 25~40 DEG C and mixing speed is 4~8d of fermentation under 20~100r/min, obtains the second hair as the tunning
Zymotic fluid.The fermentation temperature for forming the first fermentation liquid is preferably 27~30 DEG C, and mixing speed is preferably 50~80r/min, when fermentation
Between preferably 13~15d.The fermentation temperature for forming the second fermentation liquid is preferably 27~30 DEG C, and mixing speed is preferably 50~80r/
Min, fermentation time are preferably 5~7d.Whole fermentation period can not only be shortened in this way, but also can guarantee ferment effect.It is described solid
Liquid separating method can be filtering or centrifugation, be routine operation in field, no longer repeat.
Preparation process of the invention is simple, and the period is shorter, is suitble to industrialized production.
<fermented beverage and preparation method>
Fermented beverage of the invention includes fermentation clear liquid as described above and blender.As needed, the fermented beverage
It further include water.
Raw material and the preparation method of the fermentation clear liquid are as previously mentioned, which is not described herein again.
In the present invention, the blender is selected from crystal diabetin, inulin, antierythrite, cyclohexaamylose, thickener, sucrose
Aliphatic ester, zinc gluconate, xylo-oligosaccharide, caramel syrup, inspissated juice, essence, sweetener, trehalose, sodium citrate and melon
One of Rana extract is a variety of.Preferably, the blender includes crystal diabetin, inulin, antierythrite, α-ring-type paste
Essence, thickener, sucrose fatty ester, zinc gluconate, xylo-oligosaccharide, caramel syrup, inspissated juice, essence, sweetener, seaweed
Sugar, sodium citrate and Guarana extract.According to embodiment of the present invention, the blender by crystal diabetin, inulin,
Antierythrite, cyclohexaamylose, thickener, sucrose fatty ester, zinc gluconate, xylo-oligosaccharide, caramel syrup, inspissated juice,
Essence, sweetener, trehalose, sodium citrate and Guarana extract composition.The thickener can be by pectin, guar gum and lemon
Lemon acid sodium is made.Preferably, the thickener is made of pectin, guar gum and sodium citrate, and the weight ratio of three is 1~3:
6~9:1~5, preferably 2.5:8:2.The inspissated juice is the inspissated juice of fruit, and the fruit can be any fruit.It is excellent
Selection of land, the inspissated juice be selected from concentrated apple juice, concentrated pear juice, concentration kiwi-fruit juice, concentrated strawberry juice, concentrated granada juice or
Mulberry juice is concentrated.Commercial product can be directly used in above-mentioned inspissated juice.The sweetener is selected from steviol glycoside, acesulfame potassium, sweet tea
Sweet element or Aspartame.
In the present invention, it is preferable that the blender include 0.5~3 parts by weight of crystal diabetin, 0.5~3 parts by weight of inulin,
0.5~3 parts by weight of antierythrite, 0.1~0.8 parts by weight of cyclohexaamylose, 0.1~0.5 parts by weight of thickener, sucrose-fatty
0.01~0.03 parts by weight of ester, 0.001~0.02 parts by weight of zinc gluconate, 0.5~4 parts by weight of xylo-oligosaccharide, caramel syrup
0.05~4 parts by weight, 0.5~3 parts by weight of inspissated juice, 0.01~0.04 parts by weight of essence, 0.004~0.012 weight of sweetener
Measure part, 0.1~0.6 parts by weight of 0.5~4 parts by weight of trehalose, 0.05~0.5 parts by weight of sodium citrate and Guarana extract.
It is highly preferred that the blender includes 1~2.5 parts by weight of crystal diabetin, 1~3 parts by weight of inulin, 0.8~2.5 weight of antierythrite
Measure part, 0.2~0.6 parts by weight of cyclohexaamylose, 0.2~0.4 parts by weight of thickener, 0.012~0.024 weight of sucrose fatty ester
Measure part, 0.005~0.015 parts by weight of zinc gluconate, 1.5~3.5 parts by weight of xylo-oligosaccharide, 0.1~2 parts by weight of caramel syrup,
0.8~2.5 parts by weight of inspissated juice, 0.015~0.03 parts by weight of essence, 0.006~0.01 parts by weight of sweetener, trehalose
0.15~0.5 parts by weight of 0.8~2.8 parts by weight, 0.1~0.4 parts by weight of sodium citrate and Guarana extract.Tune of the invention
Ingredients can be only grouped as by the group of the parts by weight.
In the present invention, the weight ratio of fermentation clear liquid and blender can be 1:0.2~0.7, preferably 1:0.3~0.6, more
Preferably 1:0.35~0.55.The fermentation clear liquid and the weight ratio of water can be 1:0.5~3.5, preferably 1:0.55~2,
More preferably 1:0.6~1.5.
When being deployed using blender and fermentation clear liquid of the invention, antifatigue, the improvement property function of obtained fermented beverage
It can effect enhancing；It is in appearance in vivid brown；With fermentation faint scent, but the excessively strong pungent fermented flavour of fermentation clear liquid itself
Road is suitably modified and is covered, excessively not strong；Sour-sweet degree is moderate, pure flavor is easy to be consumed with good mouthfeel
Person receives；Drink system is sufficiently stable, is not easy to be precipitated, being capable of long-time storage.
The preparation method of fermented beverage of the invention include: (1) enzymolysis step, (2) mixing step, (3) fermentation step and
(4) adaptation step.
In step (1)~(3) as described above, repeating no more.
In the present invention, the step (4) can be deployed using a variety of methods.Preferably, the step (4) is specific such as
Under:
A. by the crystal diabetin, inulin, antierythrite, cyclohexaamylose and thickener are uniformly mixed and obtain premix；
B. the premix is slowly added in the water of part, water temperature is 60~80 DEG C, carries out first time stirring；Then it is added
Sucrose fatty ester carries out second and stirs, obtains mixed liquor；
C. by the fermentation clear liquid, zinc gluconate, xylo-oligosaccharide, caramel syrup, inspissated juice, essence, sweetener, seaweed
Sugar, sodium citrate, Guarana extract and remaining water are added in the mixed liquor, carry out third time stirring, filter, sterilizing,
Obtain the fermented beverage.
In the present invention, it is preferable that the mixing time of stirring is 10~50min, preferably 10~35min for the first time.Second
The mixing time of secondary stirring is 3~30min, preferably 5~20min.The mixing time of third time stirring is 10~50min, excellent
It is selected as 15~40min.
Using method of the invention, the fermented beverage of concocting method preparation especially of the invention, system is uniform and stablizes.
<purposes>
Above-mentioned fermentation clear liquid of the invention has the function of antifatigue and/or improves sexual function.Therefore, in present invention offer
It states fermentation clear liquid and is used to prepare the purposes with antifatigue and/or improvement sexual function effect drug, food or drink.Drug,
Food or drink have the meaning of this field routine, and and are not belonging to position concept up and down.It is of the present invention antifatigue including anti-Japanese
Often fatigue and resistance fatigue effect.
The test method of following embodiment is described below:
<viable count>
It is measured with reference to GB 4789.35-2016.Lactic acid bacteria is that a kind of fermentable sugars mainly generates the thin of a large amount of lactic acid
The common name of bacterium.
1, sample preparation
Whole preparation process of sample follow sterile working program.With aseptic straw after fluid sample is first sufficiently shaken up
Pipette samples 25mL is put into the sterile conical flask (preset an appropriate number of sterile glass beads in bottle) equipped with 225mL physiological saline
In, the even liquid of sample of 1:10 is made in shake well.
2, lactic acid bacterial count
B. longum counts: according to the estimation to measuring samples Bifidobacterium content, it is continuously suitable for dilute for selecting 2~3
Degree of releasing, each dilution draw the even liquid of 1mL sample in sterilizing plates, and each dilution makees two plates.Dilution moves into flat
After ware, it will be cooled to 48 DEG C of mupirocin lithium salts and the MRS culture medium of cysteine hydrochloride improvement be poured into plate about
15mL, rotation plate make to be uniformly mixed.36 ± 1 DEG C of Anaerobic culturel 72h ± 2h count all clump counts on plate after culture.
Plate is diluted to from sample pours into requirement complete in 15min.
Lactobacillus counts: according to the estimation of measuring samples total viable count, selecting 2~3 continuous acceptable diluent degree, each
Dilution draws the even liquid of 1mL sample in sterilizing plates, and each dilution makees two plates.After dilution moves into plate, it will cool down
It is poured into plate about 15mL to 48 DEG C of MRS agar mediums, rotation plate makes to be uniformly mixed.36 ± 1 DEG C of Anaerobic culturel 72h ±
2h.Plate is diluted to from sample pours into requirement complete in 15min.
Lactic acid bacteria sum: the sum of above Pseudomonas sum.
3, calculation method
Choose plate count total plate count of the clump count between 30~300CFU, without sprawling bacterium colony growth.Lower than 30CFU
Plate record specific clump count, greater than can record can not mostly to count for 300CFU.The clump count of each degree should be flat using two
The average of plate.When one plate has the growth of larger sheet bacterium colony, then it should not use, and should be with the plate of no sheet bacterium colony growth
Clump count as the dilution；If sheet bacterium colony is less than the half of plate, and bacterium colony distribution is uniformly very in remaining half
It can.It calculates after half of plate multiplied by 2, represents a flat-plate bacterial colony number.Chain without obvious boundary line between occurring bacterium colony on plate
It is when growth, then single-stranded as a bacterium colony counting by every.
If the clump count on only one dilution plate is being suitable for calculating the flat of two flat-plate bacterial colony numbers in count range
Mean value, then by average value multiplied by corresponding extension rate, as total plate count result in every gram or every milliliter.
If there are two serial dilution degree culture dish clump count be suitable for count range in when, should be carried out by following formula based on
It calculates:
N=Σ c/ (n1+0.1n2)d
Clump count in N-sample；The sum of ∑ C-culture dish (culture dish of the clump count containing optimum range) clump count；
n1--- the first dilution (low extension rate) culture dish number；n2--- the second dilution (highly diluted multiple) culture dish number；
D --- dilution gfactor (the first dilution).
If clump count is all larger than 300CFU on the plate of all dilutions, the highest plate of dilution is counted,
Other plates can record can not mostly to count, and as a result calculate by average colony number multiplied by highest extension rate.
If the flat-plate bacterial colony number of all dilutions is respectively less than 30CFU, should by the minimum average colony number of dilution multiplied by
Extension rate calculates.
If the sterile length of being born of all dilution (including fluid sample stoste) plates, with small 1 multiplied by minimum dilution times
Number calculates.
If the flat-plate bacterial colony number of all dilutions not between 30CFU~300CFU, a portion be less than 30CFU or
When big 300CFU, then calculated with the average colony number closest to 30CFU or 300CFU multiplied by extension rate.
<total acidity test>
It is measured according to GB/T 12456-2008.Using acid-base neutralization principle, with the acid in alkali-titration test solution, with
Phenolphthalein is that indicator determines titration end-point.Total acid content is calculated by the consumption of lye
<pHvalue determination >
Using the pH value of pH meter measurement fermentation liquid.
<total sugar determination>
After carbohydrate can be dehydrated generation furfural or hydroxymethylfurfural by concentrated sulfuric acid effect at relatively high temperatures, with anthrone
(C14H10O) dehydrating condensation forms the derivative of furfural, is in blue-green.The substance has absorption maximum at 620nm, in certain model
In enclosing, the depth of color is directly proportional to soluble sugar content.
1, glucose standard: 100mg glucose (powder) is weighed in dissolution in small beaker and (is first dissolved in 50ml's or so
Repeatedly rinsed when being shifted in water with water), 100ml volumetric flask constant volume (concentration of standard solution 1mg/ml).
2, the concentrated sulfuric acid (specific gravity 1.84).
3, anthrone reagent: 0.2g anthrone is dissolved in the dense H of 100ml2SO4In, the same day, which prepares, uses (concentration 0.2%).
4,6 25ml volumetric flasks are taken, a series of various concentrations (0,10 μ g/ml, 20 μ g/ml, 30 μ g/ml, 40 μ g/ are prepared
Ml, 50 μ g/ml, 60 μ g/ml) glucose solution.Taking each concentration standard liquid of 2.5ml, (blank adds 2.5ml to distill in test tube
Water), 6.5ml anthrone concentrated sulfuric acid reagent is added, mixes, is stored at room temperature one hour, the colorimetric at wavelength 620nm, reads absorbance.
Using absorbance as abscissa, concentration of standard solution is that ordinate does standard curve: Y=KX+B (μ g/ml).
5, total sugar determination
1) take 10mL sample in 50mL centrifuge tube, 8500rpm/min is centrifuged 10min.
2) take supernatant 5mL in 50mL centrifuge tube as prepare liquid.
3) extension rate is determined according to sugared concentration, be settled to 50mL, 100mL, 250mL or 500mL volumetric flask or
The good solubility of constant volume is too high to be continued to dilute V1.
4) it takes 2.5mL prepare liquid in test tube, 0.2% anthrone~concentrated sulfuric acid reagent is added, mix, be stored at room temperature one hour,
OD value is obtained at 620nm.
5) result calculates
X --- total sugar content [mg/ml] in sample；
Y --- sample measures the concentration (μ g/ml) of glucose in liquid；
V1--- sample constant volume total volume (ml)；
1000 --- μ g/ml is converted to mg/ml；
0.9 --- glucose is scaled the coefficient of Thick many candies.
Preparation example 1
It will be mixed with beating to obtain mixed liquor after leek seedling 515kg, rhizoma polygonati 118kg and Semen sesami nigrum 90kg rehydration.
Raspberry 75kg, Chinese yam 90kg are crushed respectively, 200 meshes is crossed, respectively obtains raspberry powder and yam flour.
By apple 190kg, pears 180kg, peach 160kg, banana 90kg, carrot 50kg, tomato 60kg, cucurbita pepo 40kg
It is placed in beater with broccoli 40kg, the vitamin C of the 0.5wt% of fruits and vegetables total amount is added, crushing and beating obtains fruit, vegetable juice.
Preparation example 2
It will be mixed with beating to obtain mixed liquor after leek seedling 515kg, rhizoma polygonati 118kg and Semen sesami nigrum 90kg rehydration.
By raspberry 75kg, Cordyceps militaris 12kg, Schisandra chinensis 60kg, Morinda officinalis 60kg, Herba Epimedii 80kg, Chinese yam 90kg, divide
It does not crush, crosses 200 meshes, respectively obtain raspberry powder, Cordyceps militaris powder, Fructus Schisandrae Chinensis powder, Morinda officinalis powder, Herba Epimedii powder and Chinese yam
Powder.
By apple 190kg, pears 180kg, peach 160kg, banana 90kg, carrot 50kg, tomato 60kg, cucurbita pepo 40kg
It is placed in beater with broccoli 40kg, the vitamin C of the 0.5wt% of fruits and vegetables total amount is added, crushing and beating obtains fruit, vegetable juice.
Embodiment 1
Enzymatic vessel is added in mixed liquor, raspberry powder, yam flour, the fruit, vegetable juice that preparation example 1 is obtained, and water 2300kg is added,
It is uniformly mixed and forms mixture, cellulase 8kg, pectase 8kg and acid protease 8kg is added, is digested at 55 DEG C
90min obtains enzymolysis liquid.
Enzymolysis liquid is warming up to 90 DEG C, 0.5h is extracted in enzyme deactivation, obtains enzyme deactivation extracting solution；Wheat oligopeptide 40kg is added, mixes
It closes uniformly, sodium bicarbonate is added and adjusts pH value to 6, carries out homogeneous, degassing obtains sterilizing product in 135 DEG C of sterilizing 5s.By institute
The product that must sterilize is transferred in fermentor, accesses the Lactobacillus casei 0.4kg of 320,000,000,000 CFU/g, in 28 DEG C, turn of 60r/min
The lower 15d that ferments of speed stirring, obtains the first fermentation liquid；The plant lactobacillus of 250,000,000,000 CFU/g is accessed into first fermentation liquid
The bifidobacterium adolescentis 0.2kg of 0.2kg and 300,000,000,000 CFU/g continues the 5d that ferments under the revolving speed stirring of 28 DEG C, 60r/min, obtains
To the second fermentation liquid.Second fermentation liquid is centrifuged, fermentation clear liquid is obtained.
Fermentation liquid in fermentation process is monitored, monitoring index includes viable count, total acid, pH value and total reducing sugar, as a result such as
Shown in Fig. 1~4,
As shown in Figure 1, probiotics can be roughly divided into following three phases during the fermentation:
(1) fermentation starting was to fermentation the 2nd day, and probiotics is in logarithmic growth phase, and it is numerous to enter high-speed rapid growth for probiotics at this time
It grows the stage, viable count exponentially increases and reaches highest level (10 power)；(2) it ferments the 2nd~6 day, probiotics is in steady
Periodically, viable count keeps relative stability, and maintains higher level (9~10 power)；(3) it ferments the 7th~20 day, metabolite cream
The organic acids such as acid largely accumulate, and it is horizontal (5~6 power) to be slowly drop down to initial inoculum after viable count is first rapid, but is fermenting
At the end of can still detect viable bacteria.
As shown in Figure 2, with the extension of fermentation time, total acid content is first rapidly slowly increased afterwards in fermentation liquid, until increasing
To maximum value and keep stable.Therefore, the variation of total acid content can be roughly divided into the following two stage during the fermentation:
(1) fermentation starting extremely fermentation the 5th day or so, probiotics mass propagation, and highest level is maintained, cell metabolism is prosperous
It contains.This stage total acid content increases sharply, and reaches maximum value；Total acid content can reach 33~38g/ after this general stage
L；
(2) it ferments the 7th~20 day, viable count is begun to decline, and total acid content maintains highest level, is fluctuated smaller.
From the figure 3, it may be seen that with the extension of fermentation time, fermentation liquid pH value first rapidly rear slowly decline, until minimizing
It is worth and keeps stable.Therefore, the variation of pH value can be roughly divided into the following two stage during the fermentation:
(1) fermentation starts to fermentation the 5th day, probiotics mass propagation, and maintains highest level, and cell metabolism is extremely prosperous
It contains.This stage pH value declines rapidly, and reaches minimum value；PH value can reach 3.3~3.6 after this general stage；
(2) it ferments the 6th~20 day, number of live bacteria of probiotics starts rapid decrease after first remaining constant, and thallus starts largely to split
Solution is dead, and pH value maintains minimum.
As shown in Figure 4, with the extension of fermentation time, in fermentation liquid total sugar content first rapidly after slowly decline, until drop
To minimum value and keep stable.Therefore, the variation of total sugar content can be roughly divided into the following two stage during the fermentation:
(1) fermentation starting was to fermentation the 12nd day or so, probiotics mass propagation, under first maintaining after highest level slowly
Drop, cell metabolism are vigorous.This stage total sugar content is first rapidly rear slowly to be reduced, and reaches minimum value；After this general stage
Total sugar content reaches 50~70g/L.
(2) it ferments the 13rd~20 day, probiotics is in decline phase, and viable count is down to reduced levels, and total sugar content maintains
Floor level.
Embodiment 2
A. by crystal diabetin 1.8kg, inulin 2.2kg, antierythrite 1.5kg, cyclohexaamylose 0.45kg and thickener
0.3kg is uniformly mixed, and obtains premix；
B. the premix is slowly added in 70 DEG C of the water of 12kg, carries out first time stirring, mixing time is
20min；Then sucrose fatty ester 0.02kg is added, carries out second and stirs, mixing time 10min obtains mixed liquor；
C. by the fermentation clear liquid 30L of embodiment 1, zinc gluconate 0.01kg, xylo-oligosaccharide 2kg, caramel syrup 0.2kg, dense
Contracting pear juice 1.25kg, essence 0.022kg, acesulfame potassium 0.01kg, trehalose 1.5kg, sodium citrate 0.2kg and Guarana extract
(content of caffeine is 22wt%) 0.362kg is added in the mixed liquor, adds water to 50L water, carries out third time stirring, stir
Mixing the time is 30min, is filtered, and sterilizing obtains fermented beverage.
By the fermented beverage sterile filling, sealing obtains product, and specification is 50ml/ bottles.
Embodiment 3
Mixed liquor, raspberry powder, Cordyceps militaris powder, Fructus Schisandrae Chinensis powder, Morinda officinalis powder, the Herba Epimedii powder, mountain that preparation example 2 is obtained
Enzymatic vessel is added in medicinal powder, fruit, vegetable juice, and water 2500kg is added, and is uniformly mixed and forms mixture, and cellulase 10kg, pectase is added
10kg and acid protease 8kg, digests 90min at 55 DEG C, obtains enzymolysis liquid.
Enzymolysis liquid is warming up to 90 DEG C, 0.5h is extracted in enzyme deactivation, obtains enzyme deactivation extracting solution；Wheat oligopeptide 40kg is added, mixes
It closes uniformly, sodium bicarbonate is added and adjusts pH value to 6, carries out homogeneous, degassing obtains sterilizing product in 135 DEG C of sterilizing 5s.By institute
The product that must sterilize is transferred in fermentor, accesses the Lactobacillus casei 0.4kg of 320,000,000,000 CFU/g, in 28 DEG C, turn of 60r/min
The lower 15d that ferments of speed stirring, obtains the first fermentation liquid；The plant lactobacillus of 250,000,000,000 CFU/g is accessed into first fermentation liquid
The bifidobacterium adolescentis 0.2kg of 0.2kg and 300,000,000,000 CFU/g continues the 5d that ferments under the revolving speed stirring of 28 DEG C, 60r/min, obtains
To the second fermentation liquid.Second fermentation liquid is centrifuged, fermentation clear liquid is obtained.
Embodiment 4
A. by crystal diabetin 1.8kg, inulin 2.2kg, antierythrite 1.5kg, cyclohexaamylose 0.45kg and thickener
0.3kg is uniformly mixed, and obtains premix；
B. the premix is slowly added in 70 DEG C of the water of 12kg, carries out first time stirring, mixing time is
20min；Then sucrose fatty ester 0.02kg is added, carries out second and stirs, mixing time 10min obtains mixed liquor；
C. by the fermentation clear liquid 30L of embodiment 3, zinc gluconate 0.01kg, xylo-oligosaccharide 2kg, caramel syrup 0.2kg, dense
Contracting pear juice 1.25kg, essence 0.022kg, acesulfame potassium 0.01kg, trehalose 1.5kg, sodium citrate 0.2kg and Guarana extract
(content of caffeine is 22wt%) 0.362kg is added in the mixed liquor, adds water to 50L, carries out third time stirring, stirring
Time is 30min, is filtered, and sterilizing obtains fermented beverage.
By the fermented beverage sterile filling, sealing obtains product, and specification is 50ml/ bottles.
Experimental example 1
Flavor taste test is carried out to the fermented beverage of embodiment 2 and embodiment 4, sensory evaluation criteria is referring to table 1.Sense
Official evaluates group and is made of 100 volunteers, is divided into two groups, one group of 50 people takes the fermentation of embodiment 2 and embodiment 4 respectively
Drink, test result is referring to table 2.
1 sensory evaluation criteria of table
2 test result of table
| Color | Tissue morphology | Mouthfeel | Fragrance | |
| Embodiment 2 | 23 | 25 | 23 | 24 |
| Embodiment 4 | 23 | 24 | 24 | 23 |
Note: score is the average value of 50 parts of appraisal results.
Experimental example 2- efficacy assessments
64 volunteers, which participate in, tries out experiment, is divided into two groups, every group 32, first group is tried out the fermentation that embodiment 2 obtains
Drink, second group is tried out the fermented beverage that embodiment 4 obtains.Volunteer is Married Men, the age at 33~56 years old, inferior health
State, fatiguability, sexuality are dissatisfied.
Drinking method: every volunteer drinks the fermented beverage of embodiment 2 or embodiment 4,2 times a day, 1 bottle every time
(50ml), continuous 10 days.Period normal diet.Record following project: (1) enteron aisle in terms of, including borborygmus, production gas, defecation situation；
(2) antifatigue aspect, including daily fatigue conditions and sexual life fatigue conditions；(3) in terms of sexual function, including erection function, property
Arouse, situation of ejaculating, orgasm situation and ejaculation latency totally five.
First group of experimental result:
(1) in terms of enteron aisle: 25 volunteers produce gas in each taking latter 0.5~2 hour and increase, and 7 volunteers occur
Slight borborygmus.Defecation is without exception.
(2) antifatigue aspect: 26 daily fatigue conditions improvement on volunteer's daytime, energy are promoted, and nighttime sleep quality changes
It is kind；Fatigue situation improves during 20 volunteer's sexual lifves, energetic.
(3) in terms of sexual function: 12 volunteers have three or three or more to have clear improvement in five；Separately there are 7 will
Hope person has one to two to be obviously improved in five；Two improvement of interim erection function and ejaculation latency are especially apparent.In addition,
After volunteer drinks drink, generally there is body heat phenomenon.No side effects during trying out.
The following are two typical cases therein:
Volunteer 1, male, 39 years old, drinks rear body heat, slightly produces gas, no borborygmus, stool is normally.After drinking 6 days,
Daily fatigue conditions are obviously improved, and energy is promoted, and nighttime sleep improves.After drinking 8 days, erection number increases in sexual life, penetrates
Smart prolongation of latency, situation of ejaculating and sexual arousal are obviously improved；There is morning vigorous from 7th day and gradually increases.
Volunteer 2, male, 56 years old, drink rear body heat, produce gas and increase, and slight borborygmus, urine becomes effectively, stool
Normally.Sleeping at night time length but poor quality in the past feel tired, murky daytime；After drinking 5 days, nighttime sleep gradually becomes
Deep, morning next day and energy on daytime improve, and brains is clear and bright.After drinking 7 days, ejaculation latency extends in sexual life, and sexual arousal is bright
It is aobvious to improve, there is morning vigorous within the 6th, 7,8 three day.
Second group of experimental result:
(1) in terms of enteron aisle: 27 volunteers produce gas in each taking latter 0.5~2 hour and increase, and 6 volunteers occur
Slight borborygmus.Defecation is without exception.
(2) antifatigue aspect: 25 daily fatigue conditions improvement on volunteer's daytime, energy are promoted, and nighttime sleep quality changes
It is kind；Fatigue situation improves during 17 volunteer's sexual lifves, energetic.
(3) in terms of sexual function: 16 volunteers have three or three or more to have clear improvement in five；Separately there are 12 will
Hope person has one to two to be obviously improved in five；Two improvement of interim erection function and ejaculation latency are especially apparent.In addition,
After volunteer drinks drink, generally there is body heat phenomenon.No side effects during trying out.
The following are two typical cases therein:
Volunteer 1, male, 42 years old, drinks rear body heat, slightly produces gas, there is obvious borborygmus, and stool is normal.It drinks 4 days
Afterwards, daily fatigue conditions gradually improve, and energy is promoted.After drinking 6 days, erection number more usually increases in sexual life, and ejaculation is latent
The volt phase extends, and sexual arousal is obviously improved；There is morning vigorous from 5th day and gradually increases.
Volunteer 2, male, 53 years old, drink the 3rd day body heat, perspire.It produces gas from 6th day to increase, no borborygmus.4th day
Daily energy is played to be promoted, it is not murky.There is morning vigorous phenomenon within 9th, 10 two day.
Present invention is not limited to the embodiments described above, without departing from the essence of the present invention, this field skill
Any deformation, improvement, the replacement that art personnel are contemplated that each fall within the scope of the present invention.
Claims (10)
1. a kind of with antifatigue and improvement sexual function effect fermentation clear liquid, which is characterized in that by the original including following component
Material is prepared: 500~550 parts by weight of leek seedling, 100~140 parts by weight of rhizoma polygonati, 60~100 parts by weight of raspberry, Chinese yam 60
~100 parts by weight, 60~100 parts by weight of Semen sesami nigrum, 600~1000 parts by weight of fruit, vegetable juice, 0.4~1.4 parts by weight of leavening, enzyme
2200~2700 parts by weight of 9~30 parts by weight of preparation and water.
2. fermentation clear liquid according to claim 1, which is characterized in that the raw material also comprises the following components: Cordyceps militaris 10
~20 parts by weight, 50~100 parts by weight of Herba Epimedii, 50~100 parts by weight of 50~100 parts by weight of Schisandra chinensis and Morinda officinalis.
3. fermentation clear liquid according to claim 1 or 2, which is characterized in that the fruit, vegetable juice is by following fruits and vegetables
It is obtained after crushing and beating: 150~240 parts by weight of apple, 150~240 parts by weight of pears, 100~210 parts by weight of peach, banana 50~
130 parts by weight, 20~60 parts by weight of carrot, 30~70 parts by weight of tomato, 20~45 parts by weight of cucurbita pepo and broccoli 20~
45 parts by weight.
4. fermentation clear liquid according to claim 1 or 2, which is characterized in that the enzyme preparation includes 6~10 weight of cellulase
Measure part, 6~10 parts by weight of 6~10 parts by weight of pectase and acid protease；The leavening include Lactobacillus casei 0.2~
0.1~0.35 parts by weight of 0.7 parts by weight, 0.1~0.35 parts by weight of lactobacillus plantarum and bifidobacterium adolescentis.
5. fermentation clear liquid according to claim 1 or 2, which is characterized in that the raw material further includes nitrogen source, and the nitrogen source
Dosage be 30~60 parts by weight；The nitrogen source be selected from one of wheat oligopeptide, Soyprotein peptide and yeast extract or
It is several.
6. the preparation method of described in any item fermentation clear liquids according to claim 1~5, which comprises the steps of:
(1) leek seedling, rhizoma polygonati, Semen sesami nigrum, raspberry, Chinese yam, fruit, vegetable juice and water are mixed to form mixture, it will be described mixed
It closes object to be digested under the action of enzyme preparation, obtains enzymolysis liquid；Hydrolysis temperature be 40~60 DEG C, enzymolysis time be 60~
180min；Or
By the leek seedling, rhizoma polygonati, Semen sesami nigrum, raspberry, Chinese yam, Cordyceps militaris, Herba Epimedii, Schisandra chinensis, Morinda officinalis, fruit, vegetable juice and
Water is mixed to form mixture, and the mixture is digested under the action of enzyme preparation, obtains enzymolysis liquid；Hydrolysis temperature is 40
~60 DEG C, enzymolysis time is 60~180min；
(2) enzymolysis liquid is subjected to enzyme deactivation extraction process, obtains enzyme deactivation extracting solution；Enzyme deactivation Extracting temperature is 80~100 DEG C, is gone out
Enzyme extraction time is 0.4~1.0h；
(3) it by the enzyme deactivation extracting solution, is optionally uniformly mixed with the nitrogen source, obtains mixture；By the mixture tune
PH value is saved to 5~7, sterilization treatment obtains sterilizing product；The sterilizing product is subjected to fermentation process through the leavening, is obtained
Tunning；The tunning is separated by solid-liquid separation, the fermentation clear liquid is obtained.
7. the preparation method of fermentation clear liquid according to claim 6, which is characterized in that the fermentation process of step (3)
Are as follows: Lactobacillus casei is seeded to the sterilizing product first, in temperature be 25~40 DEG C and mixing speed is 20~100r/
Ferment 12~16d under min, obtains the first fermentation liquid；Lactobacillus plantarum and bifidobacterium adolescentis are seeded to first fermentation
Liquid, ferment 4~8d in the case where temperature is 25~40 DEG C and mixing speed is 20~100r/min, obtains as the tunning
Second fermentation liquid.
8. a kind of with antifatigue and improvement sexual function effect fermented beverage, which is characterized in that it includes according to claim 1
~5 described in any item fermentation clear liquids, blender and water, the blender include crystal diabetin, inulin, antierythrite, α-ring
Shape dextrin, thickener, sucrose fatty ester, zinc gluconate, xylo-oligosaccharide, caramel syrup, inspissated juice, essence, sweetener, sea
Algae sugar, sodium citrate and Guarana extract.
9. the preparation method of fermented beverage according to claim 8, which comprises the steps of:
A. the crystal diabetin, inulin, antierythrite, cyclohexaamylose and thickener are uniformly mixed and obtain premix；
B. the premix is slowly added in the water of part, water temperature is 60~80 DEG C, carries out first time stirring；Then sucrose is added
Aliphatic ester carries out second and stirs, obtains mixed liquor；
C. by the fermentation clear liquid, zinc gluconate, xylo-oligosaccharide, caramel syrup, inspissated juice, essence, sweetener, trehalose,
Sodium citrate, Guarana extract and remaining water are added in the mixed liquor, carry out third time stirring, filter, and sterilizing obtains
The fermented beverage.
10. described in any item fermentation clear liquids are used to prepare with antifatigue and/or improvement sexual function according to claim 1~5
The purposes of the drug of effect, food or drink.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811072802.8A CN109198618A (en) | 2018-09-14 | 2018-09-14 | Fermentation clear liquid that is antifatigue, improving sexual function and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811072802.8A CN109198618A (en) | 2018-09-14 | 2018-09-14 | Fermentation clear liquid that is antifatigue, improving sexual function and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109198618A true CN109198618A (en) | 2019-01-15 |
Family
ID=64984316
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811072802.8A Pending CN109198618A (en) | 2018-09-14 | 2018-09-14 | Fermentation clear liquid that is antifatigue, improving sexual function and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109198618A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109731080A (en) * | 2019-03-22 | 2019-05-10 | 厦门元之道生物科技有限公司 | A kind of herb fermenting agent and preparation method thereof |
| CN110432484A (en) * | 2019-08-15 | 2019-11-12 | 江苏菌钥生命科技发展有限公司 | Old wheat fermentation liquid, drink and preparation method and purposes |
| CN110651919A (en) * | 2019-09-29 | 2020-01-07 | 江苏菌钥生命科技发展有限公司 | Carrot fermentation liquor and beverage for relieving asthenopia, and preparation method and application thereof |
| CN113142451A (en) * | 2021-05-12 | 2021-07-23 | 河北一然生物科技有限公司 | Chinese herbal medicine fermentation inoculant and preparation method of fermented Chinese herbal medicine beverage |
| CN116042474A (en) * | 2022-12-30 | 2023-05-02 | 华熙生物科技股份有限公司 | Lactobacillus rhamnosus and application thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20070096989A (en) * | 2007-08-10 | 2007-10-02 | 박미숙 | Manufacturing method for a fermented functional traditional oriental medicine |
| CN104435351A (en) * | 2014-11-14 | 2015-03-25 | 马静 | Traditional Chinese medicine prescribed preparation for tonifying kidneys and strengthening yang and application of traditional Chinese medicine prescribed preparation |
| CN104664270A (en) * | 2014-07-09 | 2015-06-03 | 普正药业股份有限公司 | Fresh and alive traditional Chinese medicine fruit-vegetable enzyme and preparation method thereof |
| CN105249326A (en) * | 2015-10-15 | 2016-01-20 | 维尔信科技（潍坊）有限公司 | Fermented fruit and vegetable composition containing probiotics and traditional Chinese medicine extract and preparation method of fermented fruit and vegetable composition |
| CN106306962A (en) * | 2016-08-28 | 2017-01-11 | 华南农业大学 | Anti-fatigue health drink and preparing method thereof |
| CN106912764A (en) * | 2017-03-08 | 2017-07-04 | 南京工业大学 | Method for preparing traditional Chinese medicine fruit and vegetable enzyme drink |
| CN107173656A (en) * | 2017-06-29 | 2017-09-19 | 马明磊 | A kind of Chinese medicine fruits and vegetables probiotics fermented beverage and preparation method thereof |
| CN108094792A (en) * | 2017-12-27 | 2018-06-01 | 天津科技大学 | A kind of probiotics fermention functional food for improving male's inferior health and preparation method thereof |
| CN108477608A (en) * | 2018-03-06 | 2018-09-04 | 安徽养生天下生物科技有限公司 | A kind of ferment synthesis drink of establishing-Yang and preparation method thereof |
-
2018
- 2018-09-14 CN CN201811072802.8A patent/CN109198618A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20070096989A (en) * | 2007-08-10 | 2007-10-02 | 박미숙 | Manufacturing method for a fermented functional traditional oriental medicine |
| CN104664270A (en) * | 2014-07-09 | 2015-06-03 | 普正药业股份有限公司 | Fresh and alive traditional Chinese medicine fruit-vegetable enzyme and preparation method thereof |
| CN104435351A (en) * | 2014-11-14 | 2015-03-25 | 马静 | Traditional Chinese medicine prescribed preparation for tonifying kidneys and strengthening yang and application of traditional Chinese medicine prescribed preparation |
| CN105249326A (en) * | 2015-10-15 | 2016-01-20 | 维尔信科技（潍坊）有限公司 | Fermented fruit and vegetable composition containing probiotics and traditional Chinese medicine extract and preparation method of fermented fruit and vegetable composition |
| CN106306962A (en) * | 2016-08-28 | 2017-01-11 | 华南农业大学 | Anti-fatigue health drink and preparing method thereof |
| CN106912764A (en) * | 2017-03-08 | 2017-07-04 | 南京工业大学 | Method for preparing traditional Chinese medicine fruit and vegetable enzyme drink |
| CN107173656A (en) * | 2017-06-29 | 2017-09-19 | 马明磊 | A kind of Chinese medicine fruits and vegetables probiotics fermented beverage and preparation method thereof |
| CN108094792A (en) * | 2017-12-27 | 2018-06-01 | 天津科技大学 | A kind of probiotics fermention functional food for improving male's inferior health and preparation method thereof |
| CN108477608A (en) * | 2018-03-06 | 2018-09-04 | 安徽养生天下生物科技有限公司 | A kind of ferment synthesis drink of establishing-Yang and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 何国庆等: "《食品微生物学》", 31 August 2002, 中国农业大学出版社 * |
| 秦竹等: "《中医食疗养生学》", 31 October 2017, 中国中医药出版社 * |
| 苏瑾: "《中草药提取分离技术原理与应用》", 31 August 2015, 西安交通大学出版社 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109731080A (en) * | 2019-03-22 | 2019-05-10 | 厦门元之道生物科技有限公司 | A kind of herb fermenting agent and preparation method thereof |
| CN109731080B (en) * | 2019-03-22 | 2021-09-24 | 厦门元之道生物科技有限公司 | Traditional Chinese medicine leavening agent and preparation method thereof |
| CN110432484A (en) * | 2019-08-15 | 2019-11-12 | 江苏菌钥生命科技发展有限公司 | Old wheat fermentation liquid, drink and preparation method and purposes |
| CN110651919A (en) * | 2019-09-29 | 2020-01-07 | 江苏菌钥生命科技发展有限公司 | Carrot fermentation liquor and beverage for relieving asthenopia, and preparation method and application thereof |
| CN113142451A (en) * | 2021-05-12 | 2021-07-23 | 河北一然生物科技有限公司 | Chinese herbal medicine fermentation inoculant and preparation method of fermented Chinese herbal medicine beverage |
| CN116042474A (en) * | 2022-12-30 | 2023-05-02 | 华熙生物科技股份有限公司 | Lactobacillus rhamnosus and application thereof |
| CN116042474B (en) * | 2022-12-30 | 2024-07-26 | 华熙生物科技股份有限公司 | Lactobacillus rhamnosus and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109198618A (en) | Fermentation clear liquid that is antifatigue, improving sexual function and its preparation method and application | |
| CN102352299B (en) | Jewel orchid fruit vinegar and jewel orchid fruit vinegar beverage | |
| CN103653171B (en) | Preparation method for radix puerariae lactobacillus effervescent tablet | |
| CN109880732A (en) | A kind of relieving alcoholism and protecting liver fruit vinegar beverage and preparation method thereof | |
| CN104643225A (en) | Liquorice vegetable drink for improving gastrointestinal function and enhancing immunity and preparation method of liquorice vegetable drink | |
| CN110432485A (en) | Dendrobium candidum fermentation liquid, drink and preparation method and purposes | |
| CN104664512A (en) | Licorice plant drink capable of enhancing immunity as well as preparation method thereof | |
| CN104187930B (en) | Bitter bamboo shoot juice drink and preparation method thereof | |
| KR20130002690A (en) | Making method for fermentation beverage of rubus coreanus | |
| CN111493273B (en) | Preparation method of Polygonatum kingianum fermentation liquor | |
| CN104543822A (en) | Preparation method of fermented traditional Chinese dealcoholic functional beverage | |
| CN114376040A (en) | Xinhui mandarin kangpu tea and preparation method thereof | |
| CN110250507A (en) | A kind of Herba Cistanches ferment and preparation method | |
| CN117137131A (en) | Polygonatum sibiricum and wolfberry enzyme powder and preparation method thereof | |
| CN108671089A (en) | A kind of meat glossy ganoderma composition and its application in alzheimer's disease | |
| CN104664513A (en) | Licorice plant beverage and preparation method thereof | |
| CN106858589A (en) | A kind of lycium ruthenicum compressing tablet and preparation method thereof | |
| CN115005437B (en) | Ferment beverage with effects of dispelling effects of alcohol and protecting liver and preparation method thereof | |
| CN105661399A (en) | Blueberry composite powder and preparation method thereof | |
| CN113907231B (en) | Preparation method of mulberry leaf biological beverage and product | |
| CN108524901A (en) | Alleviate the fermentation clear liquid and its preparation method and application of palace cold | |
| CN109259223A (en) | A kind of method of cloud ginseng ferment processing | |
| CN116965542A (en) | Hawthorn fermentation product and preparation method and application thereof | |
| CN108813255A (en) | Fermentation clear liquid, drink and preparation method and purposes | |
| CN106036317A (en) | Asparagus fermented beverage with auxiliary hypolipidemic function and preparation method of asparagus fermented beverage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information | ||
| CB02 | Change of applicant information |
Address after: 224100 No. 51 Nanxiang West Road, Dafeng District, Yancheng City, Jiangsu Province Applicant after: JIANGSU JUNYAO LIFE TECHNOLOGY DEVELOPMENT Co.,Ltd. Address before: 224100 No. 51 Nanxiang West Road, Dafeng District, Yancheng City, Jiangsu Province Applicant before: JIANGSU LIJIAN LIFE TECHNOLOGY DEVELOPMENT CO.,LTD. |
|
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190115 |