CN109645488A - A kind of Moringa dietary fiber and preparation method thereof application - Google Patents

A kind of Moringa dietary fiber and preparation method thereof application Download PDF

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CN109645488A
CN109645488A CN201811557494.8A CN201811557494A CN109645488A CN 109645488 A CN109645488 A CN 109645488A CN 201811557494 A CN201811557494 A CN 201811557494A CN 109645488 A CN109645488 A CN 109645488A
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moringa
dietary fiber
fermentation
preparation
slag
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田洋
盛军
陶亮
赵存朝
杨舒文
解静
史崇颖
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Yunnan Agricultural University
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Yunnan Agricultural University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21Addition of substantially indigestible substances, e.g. dietary fibres
    • A23L33/22Comminuted fibrous parts of plants, e.g. bagasse or pulp
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16Inorganic salts, minerals or trace elements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/19Dairy proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The invention discloses a kind of Moringa dietary fibers and its preparation method and application.The preparation method operation is as follows: then high pressure sterilization after the pretreatment of Moringa slag accesses mycotic spore suspension and ferments, secondary high-pressure sterilizing is carried out after mold fermentation, then accesses lactic acid bacteria and carry out secondary fermentation;High-pressure homogeneous after secondary fermentation, vacuum freeze drying, crushing obtain Moringa dietary fiber, and the mould is aspergillus niger and trichoderma reesei, and the lactic acid bacteria is lactobacillus plantarum and lactobacillus acidophilus.Operation of the present invention is simple, and cost is relatively low, and preferable dietary fiber modification may be implemented by microbial fermentation processes stage by stage, substantially increase the content of Soluble Fiber, product sensory and physicochemical property are improved, product quality is high, is suitable for large-scale industrial production application.

Description

A kind of Moringa dietary fiber and preparation method thereof application
Technical field
The invention belongs to food processing technology field more particularly to a kind of Moringa dietary fiber and preparation method thereof and answer With.
Background technique
One word of dietary fiber is proposed that people claim it to be " the seventh-largest nutrient ", it is one by Hipsley in nineteen fifty-three earliest Class cannot be digested and assimilated by human small intestine, can be in the edible vegetable ingredients that human body large intestine can partly or entirely ferment, i.e., The summation of carbohydrate and similar substance, including polysaccharide, oligosaccharides, lignin and relevant plant material.
Water-soluble dietary fiber (SDF) and water insoluble dietary fiber (IDF) can be divided into according to dissolubility in water Two major classes.Insoluble diedairy fiber refers to the not part dietary fiber by human body alimentary canal enzymic digestion and insoluble in hot water, is The main component of cell wall is constituted, chitin and shell including cellulose, hemicellulose, lignin, protopectin and animality are poly- Sugar, lignin are not belonging to polysaccharide, are the aromatic hydrocarbons for making cell wall keep certain toughness.Aqueous soluble dietary is fine Dimension refers to not by human consumption's enzymic digestion, but is dissolved in warm water or hot water and its water solubility again by the part diet of ethyl alcohol reprecipitation Fiber.It mainly include pectin, glucan, guar gum and fungi polysaccharide etc..By the source point of dietary fiber, plant origin can be divided into Dietary fiber, animality source dietary fiber, seaweeds polysaccharide dietary fiber, microbial polysaccharide, the semi-synthetic class diet of synthesis The different dietary fiber in the sources such as fiber.Its chemical nature is widely different, but ultimate constituent is then more similar, distinguishes main table The relative amount of present each constituent, the difference in terms of the glycosidic linkage of molecule, the degree of polymerization and branched structure.
The short chain that new research discovery physiological function of diet fiber and its fermenting property and fermentation in enteron aisle generate The amount of fatty acid is closely related.The dietary fiber of natural source is not able to satisfy people in certain operational characteristiies or functional characteristic Requirement.By distinct methods improve dietary fiber physicochemical characteristics and it by intestinal flora ferment generate short-chain fat The quantity or mode of acid, are conducive to the functional promotion of dietary fiber.For example it is improved using extrusion cooking soluble in bean dregs For the content of dietary fiber to promote its physiological function, this research increases soluble dietary fibre content by other means also class As effect.
Although human body cannot directly digest and assimilate dietary fiber, it can indirectly work to human body, get in recent years To get over the concern by nutritionist.Insoluble diedairy fiber has prevention obesity, constipation, colon cancer and other effects, soluble Dietary fiber can reduce serum cholesterol, inhibit postprandial blood sugar, improve intestinal flora.Dietary fiber is keeping digestive system strong It plays important role in health, there is enhancing digestive function, protection alimentary canal and the effect that prevents colon cancer.Absorb enough meals Food fiber can also prevent cardiovascular disease, cancer, diabetes and Other diseases;Because its can slow down digestion rate and most quickly Excretion of cholesterol has outstanding behaviours in terms of losing weight and reducing blood cholesterol levels.Intake of multiple countries to it at present Relevant regulations are done.The World Food Programme suggests that normal population intake dietary fiber amount is 27 grams/day;Food section, the European Economic Community Committee proposed standard is 30 grams/day, and the intake for the total dietary fiber that U.S. FDA is recommended is 20~35g/d of adult, adds and takes The intake for recommending everyone dietary fiber greatly is 22~24g/d.Chinese Soclety of Nutrition proposes that adult's dietary fiber is suitable for 2000 Preferably take in 30.2g/d.But daily practical intake is only 12g or so per capita in China, for Chinese Consumer's, is needed daily The amount for wanting complementary diets fiber is about 15~20g.
Dietary fiber is in enteron aisle easily by bacterium glycolysis, and wherein Soluble Fiber can be completely by bacterium glycolysis, and insoluble meals Food fiber is then not easy by glycolysis.And the short chain fatty acids generated after glycolysis such as ethyl ester acid, propyl ester acid and butyl ester acid can be used as intestines The energy source of road cell and bacterium.It can promote intestines peristalsis, reduce flatulence, improve constipation.Water-soluble dietary fiber, which has, to be mentioned High intestinal calcium absorption, calcium balance and bone mineral density.Therefore promote in dietary fiber insoluble diedairy fiber to soluble meals Food fiber conversion is of great significance.
It is mainly made of pectin, soluble dietary fiber and water insoluble dietary fiber in Moringa dietary fiber, but solvable The content of property dietary fiber is extremely low, therefore certain methods need to be taken to be modified water-insoluble diet, changes it more At soluble dietary fiber, to improve its utility value.But how there has been no document reports at present will be insoluble in Moringa fiber Property dietary fiber is converted into soluble dietary fiber, and can preferably improve the physics and chemistry and organoleptic attribute of dietary fiber.
Summary of the invention
The purpose of the present invention is to provide a kind of Moringa dietary fibers and its preparation method and application.The present invention utilizes Moringa Slag is raw material, is sterilized using mould, the stage fermentation process material combination secondary high-pressure of lactic acid bacteria, the object of high-pressure homogeneous crusher machine Reason method not only solves adding for the particulate breakup of insoluble diedairy fiber to the mode of the particulate breakup of insoluble diedairy fiber Work problem at high cost, also transformation dietary fiber existence form, improve the content of soluble dietary fiber in Moringa dietary fiber.
First aspect present invention, a kind of preparation method of Moringa dietary fiber, the preparation method operation are as follows: Moringa slag Then high pressure sterilization after pretreatment accesses mycotic spore suspension and ferments, secondary high-pressure sterilizing carried out after mold fermentation, then connect Enter lactic acid microzyme secondary fermentation;High-pressure homogeneous after secondary fermentation, vacuum freeze drying, crushing obtain Moringa dietary fiber, described mould Bacterium is aspergillus niger and trichoderma reesei, and the lactic acid bacteria is lactobacillus plantarum and lactobacillus acidophilus.The Moringa slag derives from Moringa Residue after leaf converted products.
Further, the mycotic spore suspension preparation process is as follows: will access the aspergillus niger and Richter scale of slant medium After inclined-plane covers with spore, with aseptic water washing inclined-plane, bead dispersal spore is added in trichoderma 60~90h of culture, appropriate dilute It releases, is counted with blood counting chamber, make spore concentration 108~109CFU/mL。
Further, aspergillus niger and Li's Trichoderma strain ratio 1:1~1:3 in the mycotic spore suspension, when fermentation Mycotic spore suspension accounts for the 1%~8% of quality of material.
Further, mold fermentation process is as follows: Moringa slag is prepared into semi-solid solution height according to solid-liquid ratio 2:1~1:2 Pressure sterilizing after be laid in stainless steel iron pan, material with a thickness of 0.5~3.0cm, by the aspergillus niger and trichoderma reesei after domestication Bacterium inoculation bacterium solution be uniformly sprayed on material according to strain ratio 1:1~1:3, inoculation bacterium solution quality account for quality of material 1%~ 8%, one layer of preservative film is then covered, is put into 20~40 DEG C of incubator or greenhouse and ferments, fermentation overturn object after 3~10 days Material, continues fermentation 5~14 days.
Selectively, mold fermentation process is as follows: high pressure is gone out after Moringa slag is dissolved in water according to solid-liquid ratio 1:5~1:20 Bacterium is then transferred in fermentor, by after domestication aspergillus niger and Li's Trichoderma inoculation bacterium solution according to strain ratio 1:1~1: 3 are added into fermentor, and inoculation bacterium solution quality accounts for the 1%~8% of quality of material, and oxygenating is fermented, and mixing speed setting 50~ 120r/min, fermentation temperature are 28~37 DEG C, and fermentation time is 3~10 days.
Further, the lactic acid bacteria fermentation process is as follows: material moisturizing to the solid-liquid ratio after collecting mold fermentation is 1:5 ~1:20, while addition accounts for the whey powder of material dry weight 1~5%, slowly stirring is until dissolution, then 100 DEG C~125 DEG C high temperature Lactobacillus plantarum and lactobacillus acidophilus after being cooled to 25~40 DEG C of inoculation domestications under condition of high voltage after sterilizing, 5~60min of enzyme deactivation Bacterium solution, bacterium solution quality are the 2%~10% of fermentation liquid gross mass, and the strain ratio of lactobacillus plantarum and lactobacillus acidophilus is 0.8 ~1.2:0.8~1.2, fermentation time are for 24 hours~72h.
Further, the Moringa slag preprocessing process is as follows: the Moringa slag of collection is dried under the conditions of 55 DEG C~75 DEG C It is dry, the Moringa slag flour mill after being dried to moisture content lower than 8.0% or pulverizer are crushed, pressed after crossing 80~120 meshes According to Moringa ground-slag: the ratio of water 1:5~20 is added 45~85 DEG C of warm water and is stirred evenly.
Further, autoclave conditions are as follows: sterilize 15~20min at 115~125 DEG C, and temperature is down to after sterilizing 50 DEG C or less are opened autoclave again, avoid depositing because of Moringa ground-slag and spray bottle caused by container bottom is reduced rapidly because of air pressure and temperature The phenomenon that and lose Moringa sample.
Second aspect of the present invention provides the Moringa dietary fiber that the preparation method obtains.
Third aspect present invention provides the Moringa dietary fiber and is preparing the application in Moringa dietary fiber lozenge, institute State the ingredient that Moringa dietary fiber lozenge includes following parts by weight: 50~70 parts of Moringa dietary fiber dry powder, mangrove bark dry powder 10 ~20 parts, 10~15 parts of whey powder, 0.5~1.0 part of stevioside, 0.5~3.0 part of maltodextrin, 0.1~1.0 part of magnesium stearate And 10~20 parts of drinkable water.Moringa dietary fiber lozenge carries and convenient, and rich in there is dietary fiber, having improves enterobacteriaceae Group, prevent constipation and anticancer and other effects;Lozenge flavor is adjusted in the mangrove bark dry powder of addition simultaneously, and palatability is good.
The features of the present invention is as follows: dietary fiber content is higher in Moringa residue, in Moringa dietary fiber mainly by pectin, Soluble dietary fiber and water insoluble dietary fiber are constituted, but the content of soluble dietary fiber is extremely low, water-insoluble fiber It is not easy to be absorbed by the body again, in addition Moringa bitter taste sense is stronger.To solve the above-mentioned problems, water-soluble dietary fiber is improved Content, the present invention using mould (the aspergillus niger and trichoderma reesei) fermentation after domestication, generate cellulase, hemicellulose first Enzyme, dextranase, pectase etc..Since insoluble diedairy fiber is to pass through glycosidic bond and another molecule by glucopyranosyl Hydroxyl, between amido or sulfydryl be condensed the huge molecule long-chain that acetal bonds or ketal key of formation etc. connect, due to Portugal The Hyarogen-bonding that polysaccharide chains are interior, interchain is strong, insoluble diedairy fiber are tight in the arrangement of crystalloid microfiber bundle structural unit It is close, sound construction.The various enzymes that mold fermentation process generates can make Moringa solid impurity particle become smaller, short texture, preliminary to reduce water not The degree of polymerization and molecular weight of soluble fiber;Then lactic acid microzyme secondary fermentation is used again, and lactobacillus-fermented constantly generates the generations such as lactic acid It thanks to product, so that the Moringa slag long period is in acid condition, the glycosidic bond of cellulose is further broken in acid condition, simultaneously New reducing end under neutral is generated, the macromolecular polymeric degree of Moringa dregs diet fibre also constantly declines, is partially converted into non-digestible Solvable polysaccharide (soluble dietary fiber), to improve the content of soluble dietary fiber.In addition, can be changed significantly by fermentation The bitter mouthfeel of kind Moringa, the especially generation of lactic acid etc., the taste of reconciliation Moringa dietary fiber connect it easily for people By.High pressure homogenization is carried out after batch fermentation again, the impact capacity of high pressure homogenizer is big, can be by Moringa dietary fiber particles into one Step crushes, and can further improve the mouthfeel of Moringa dietary fiber.
Compared with prior art, the invention has the following advantages: operation of the present invention is simple, cost is relatively low, by dividing Preferable dietary fiber modification may be implemented in stage microbial fermentation processes, substantially increases the content of Soluble Fiber, improves Product sensory and physicochemical property, product quality is high, is suitable for large-scale industrial production application.
Specific embodiment
Further details of the technical solution of the present invention combined with specific embodiments below, but the present invention does not limit to In following technical scheme.
Embodiment 1
The fabrication processing of Moringa water-soluble fibre is as follows:
Milling → plus water mixing → high pressure sterilization → access mycotic spore suspension fermentation → secondary height after Moringa slag → drying Press sterilizing → access lactobacillus-fermented → high-pressure homogeneous → freeze-drying → ultramicro grinding.
Strain prepares
(1) bacteria selection
It is fine that Moringa diet is extracted in Moringa residue after patent (107549817 A of CN) to be produced to the natural organic calcium of Moringa Dimension, pulverizer crushes after drying, dissolves according to the solid-liquid ratio of 1:5, is reference index from a variety of using water soluble dietary fiber content Lactic acid bacteria (lactobacillus plantarum, Lactobacillus casei, lactobacillus fermenti, Lactobacillus helveticus, Yue Shi lactobacillus, lactobacillus paracasei, sieve Her formula lactobacillus, Lactobacillus salivarius, lactobacillus acidophilus, lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium adolescentis etc. 15 Strain) in screening obtain optimal 2 plants of bacterium: lactobacillus plantarum, lactobacillus acidophilus.From mould (aspergillus oryzae, Rhizopus oryzae, Rhizopus oligosporus, Trichoderma reesei, aspergillus niger, monascus) in screening obtain optimal 2 plants of bacterium: aspergillus niger, trichoderma reesei;The bacterium used in the present invention Strain is that Chinese industrial Microbiological Culture Collection administrative center (CICC) and Guangdong Province's Culture Collection are commercially available.
(2) actication of culture and domestication
The activation of mold species selects potato juice culture medium, and ingredient is as follows: potato 200g, glucose 20g, water 1000mL adjusts pH to 6.5 or so.By the Moringa ground-slag after dries pulverizing by account for culture medium dry weight 1%, 3%, 5%, 8%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100% it is added step-wise to potato culture (shaking flask culture) is tamed in solution, each gradient domestication culture 72h.
Lactic acid bacteria activation uses MRS broth bouillon, and ingredient is as follows: peptone 10g, beef extract powder 5g, Tween 80 1mL, Dipotassium hydrogen phosphate 2g, yeast extract 4g, magnesium sulfate 0.2g, Triammonium citrate 2g, glucose 20g, manganese sulfate 0.05g, sodium acetate 5g, 1000mL water adjust pH to 6.2 or so.By the Moringa ground-slag after dries pulverizing by account for culture medium dry weight 1%, 3%, 5%, 8%, 10%, 15%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100% it is added step-wise to MRS Culture, each gradient domestication culture 48h are tamed in broth bouillon solution.
The preparation of Moringa water-soluble fibre
(1) Moringa slag: residual after the Moringa centrifugation after patent (107549817 A of CN) to be produced to the natural organic calcium of Moringa Slag collection is used to prepare Moringa diet fiber product.
(2) drying milling: the Moringa residue of collection is dried under cryogenic, and set temperature is 55 DEG C~75 DEG C.It will Leaf of Moringa after moisture content is dried to lower than 8.0% is crushed with flour mill or pulverizer, and it is spare to cross 80~120 meshes.
(3) plus water mixes: according to Moringa ground-slag: ratio addition warm water (45~55 DEG C) of water 1:1~1.5 stirs evenly.
(4) high pressure sterilization: high pressure is transferred to after Moringa aqueous solution being fitted into suitable glass or stainless steel ware and is gone out It sterilizes in bacterium pot.Sterilising conditions are the 15~20min that sterilizes at 121 DEG C, pay attention to sterilizing allowing to open after pot temperature is down to 50 DEG C and go out Bacterium pot avoids depositing because of Moringa ground-slag and container bottom loses Moringa due to air pressure and temperature reduce rapidly the phenomenon that causing spray bottle Sample.
(5) mold fermentation
Spore suspension preparation: aspergillus niger, the trichoderma reesei culture 72h of slant medium will be accessed, covers with spore to inclined-plane Afterwards, with aseptic water washing inclined-plane, bead dispersal spore is added, it is appropriate to dilute, it is counted with blood counting chamber, spore concentration is made to exist 108~109CFU/mL。
Inoculation fermentation method: add water to be prepared into semi-solid solution according to solid-liquid ratio 1:1 smashed Moringa slag, be laid in In stainless steel iron pan, the thickness of material is in 0.5~3.0cm thickness, most preferably with a thickness of 1.0cm, by the aspergillus niger after domestication, Richter scale Trichoderma is uniformly sprayed on material (inoculation bacterium solution quality accounts for the 4% of quality of material) according to strain ratio 1:1, then gently The upper one layer of preservative film of cover, fermentation temperature be 28~32 DEG C, fermentation time be 7~8d, overturn material 1 time, continue fermentation 5~ 7d。
(6) secondary high-pressure sterilizes: the fermentation liquid autoclave sterilization of the Moringa slag after mold fermentation sterilizes at 121 DEG C 15~20min.
(7) lactobacillus-fermented
Material moisturizing to solid-liquid ratio after collecting mold fermentation is 1:8, while addition accounts for 2~3% cream of material dry weight Clear powder, slowly stirring is until dissolution, then sterilizing, enzyme deactivation under high-temperature and high-pressure conditions, optimal sterilizing, enzyme deactivation condition are 110 DEG C The lactobacillus plantarum after inoculation is tamed at 37 DEG C~42 DEG C, lactobacillus acidophilus are cooled to after lower high pressure sterilization 10min, strain amount is 4~10% (lactobacillus plantarums, lactobacillus acidophilus 1:1) of the bacterium solution quality for fermentation liquid gross mass, optimum inoculation amount 6%, most Good fermentation time is 48h.
Lactobacillus-fermented constantly generates the metabolites such as lactic acid, so that the Moringa pomace long period is in acid condition, in acid Property under the conditions of cellulose glycosidic bond fracture, generate new reducing end under neutral, the macromolecular polymeric degree of Moringa dregs diet fibre Constantly decline, is partially converted into non-digestible solvable polysaccharide (soluble dietary fiber), to improve soluble dietary fiber Content.A small amount of skimmed milk power is added in experiment, and lactobacillus-fermented needed nutrient matter is provided.
(8) high-pressure homogeneous: to improve dietary fiber mouthfeel and need that partial size is made to become smaller, (generated by the stage fermentation of microorganism Cellulase, hemicellulase, dextranase, pectase etc.) so that Moringa solid impurity particle is become smaller, short texture, it can obviously improve peppery The wooden dietary fiber mouthfeel.At the same time, the impact capacity of high pressure homogenizer is big, can be by the further powder of Moringa dietary fiber particles It is broken, and then improve its mouthfeel.
(9) vacuum freeze drying: the material after homogeneous is taken out, and is put into freeze-drying disk, it is dry to be transferred to vacuum refrigeration In dry machine, it is frozen into solid-state at -10 DEG C~-50 DEG C, 20~40h is freeze-dried at vacuum (1.3~13Pa) keeps moisture direct It is sublimed into gaseous state, makes material dewatering, obtains dietary fiber dry powder.The moisture content of freeze-drying Moringa dietary fiber in the present invention≤ 5.0%.Vacuum freeze drying processing sample can make the edibility of product and mouthfeel become good.
(10) pulverize: the sample after freeze-drying is placed it in after common grinder crushes and is transferred in micronizer, It is crushed to 200~300 mesh.
Moringa sample characteristics of for example variation before and after the processing and fiber content variation are as shown in Table 1 and Table 2 respectively.
Table 1
Swellability and retention ability are to measure two important indicators of dietary fiber quality.Utilize microbial fermentation Moringa slag system Standby Moringa dietary fiber, achievees the purpose that be modified Moringa dietary fiber.Treated holds oily power for Moringa dietary fiber 1.06g/g, dilatancy 5.96mL/g, retention ability 4.64g/g, be respectively increased 47.22% before relatively handling, 42.28%, 63.96%;Effective grain size is 397.4nm, and molecule particles become smaller, 36.31% before partial size only accounts for processing that treated;After processing The dispersion index of sample is 0.11, improves dispersibility, the cellulase for generation of mainly fermenting keeps the original degree of polymerization high Diet structure is degraded to loose structure, i.e., fermentation in combining physical technique, which handles Moringa slag, can be improved the water holding of insoluble diedairy fiber Power, in conjunction with physicochemical characteristics such as waterpower, expansive forces, the specific surface area of dietary fiber increases, and dispersion degree increases, and enhances the physiology of product Function improves the organoleptic quality of dietary fiber.It especially improves the expansive force of dietary fiber and retention ability is improvement its function spy The important embodiment of property.
Dietary fiber content changes table 2 before and after the processing
As shown in Table 2, the composition ratio of dietary fiber changes in Moringa after processing, water soluble dietary fiber content from 10.17% improves to 24.48%, is increased to original 2.4 times, optimizes dietary fiber structure, is more advantageous to raising effect effect Fruit.Meanwhile the product characteristic of Moringa dietary fiber is optimized, and helps to improve intestinal flora, and have the work of gut purge, defaecation With product quality is higher, improves Moringa value-added content of product and competitiveness.
Embodiment 2
Unlike the first embodiment, the present embodiment carries out mold fermentation using inoculation fermentation method: by smashed Moringa Slag is transferred in fermentor after being dissolved in water according to solid-liquid ratio 1:8~10, and aspergillus niger, the Li's Trichoderma after domestication are added into In fermentor (inoculation bacterium solution quality amount accounts for the 5~6% of quality of material), oxygenating fermentation, mixing speed sets 50~120r/min Fermentation temperature is 33~35 DEG C, and fermentation time is 7~8d.Other operations are the same as embodiment 1.
Dietary fiber content variation is as shown in table 3 before and after the present embodiment Moringa Slag treatment.
Dietary fiber content changes table 3 before and after the processing
As shown in Table 3, the composition ratio of dietary fiber changes in Moringa after processing, water soluble dietary fiber content from 10.17% improves to 26.35%, is increased to original 2.6 times.
Embodiment 3
Based on parts by weight, 60 parts of Moringa dietary fiber dry powder prepared in the above embodiments, 15 parts of mangrove bark dry powder, cream are taken Clear 12 parts of powder, 0.8 part of stevioside, 2 parts of maltodextrin, 0.5 part of magnesium stearate, 14 parts of drinkable water, part is mixed according to the proportion The mixing speed for being placed on blender is 40~60r/min, and 1~3h of stirring is uniformly mixed, and must mix material, will mix material and is granulated, Then it dries and the dry particl that water content is lower than 1.0% is made, it is last to select corresponding mold according to containing plate shape, size, weight It is placed in tabletting in tablet press machine.It can be made into the product of corresponding package as needed, including packed, box-packed etc..According to national product matter Amount standard detects product quality, and wherein dietary fiber content is not less than 55%, dispatches from the factory after qualified.

Claims (10)

1. a kind of preparation method of Moringa dietary fiber, which is characterized in that the preparation method operation is as follows: the pretreatment of Moringa slag Then high pressure sterilization afterwards accesses mycotic spore suspension and ferments, secondary high-pressure sterilizing carried out after mold fermentation, then access lactic acid Microzyme secondary fermentation;High-pressure homogeneous after secondary fermentation, vacuum freeze drying, crushing obtain Moringa dietary fiber, and the mould is black Aspergillus and trichoderma reesei, the lactic acid bacteria are lactobacillus plantarum and lactobacillus acidophilus.
2. the preparation method of Moringa dietary fiber as described in claim 1, which is characterized in that the mycotic spore suspension preparation Process is as follows: the aspergillus niger and trichoderma reesei 60~90h of culture of slant medium will be accessed, after inclined-plane covers with spore, with nothing Bacterium water rinses inclined-plane, and bead dispersal spore is added, appropriate to dilute, and makes spore concentration 10 with blood counting chamber counting8~ 109Within the scope of CFU/mL.
3. the preparation method of Moringa dietary fiber as claimed in claim 2, which is characterized in that black in the mycotic spore suspension Aspergillus and Li's Trichoderma strain ratio 1:1~1:3, mycotic spore suspension accounts for the 1%~8% of quality of material when fermentation.
4. the preparation method of Moringa dietary fiber as claimed in claim 2, which is characterized in that mold fermentation process is as follows: institute It states Moringa slag and is prepared into semi-solid solution according to solid-liquid ratio 2:1~1:2, be laid in after high pressure sterilization in stainless steel iron pan, material With a thickness of 0.5~3.0cm, by after domestication aspergillus niger and Li's Trichoderma inoculation bacterium solution it is equal according to strain ratio 1:1~1:3 Even to be sprayed on material, inoculation bacterium solution quality accounts for the 1%~8% of quality of material, then covers one layer of preservative film, is put into 20~40 DEG C incubator or greenhouse in ferment, fermentation 3~10 days after overturn material, continue fermentation 5~14 days.
5. the preparation method of Moringa dietary fiber as claimed in claim 2, which is characterized in that mold fermentation process is as follows: institute High pressure sterilization after Moringa slag is dissolved in water according to solid-liquid ratio 1:5~1:20 is stated, is then transferred in fermentor, it will be black after domestication Aspergillus and Li's Trichoderma inoculation bacterium solution are added into fermentor according to strain ratio 1:1~1:3, and inoculation bacterium solution quality accounts for material The 1%~8% of quality, oxygenating fermentation, mixing speed set 50~120r/min, and fermentation temperature is 28~37 DEG C, fermentation time It is 3~10 days.
6. the preparation method of Moringa dietary fiber as described in claim 1, which is characterized in that the lactic acid bacteria fermentation process is such as Under: material moisturizing to the solid-liquid ratio after collecting mold fermentation is 1:5~1:20, while addition accounts for the whey of material dry weight 1~5% Powder, slowly stirring is cooled to 25 after sterilizing, 5~60min of enzyme deactivation under dissolution, then 100 DEG C~125 DEG C high-temperature and high-pressure conditions ~40 DEG C inoculation domestication after lactobacillus plantarums and lactobacillus acidophilus bacterium solution, bacterium solution quality be fermentation liquid gross mass 2%~ 10%, the strain ratio of lactobacillus plantarum and lactobacillus acidophilus is 0.8~1.2:0.8~1.2, and fermentation time is for 24 hours~72h.
7. the preparation method of Moringa dietary fiber as described in claim 1, which is characterized in that the Moringa slag preprocessing process It is as follows: the Moringa slag of collection to be dried under the conditions of 55 DEG C~75 DEG C, by the Moringa slag after being dried to moisture content lower than 8.0% Crushed, crossed after 80~120 meshes according to Moringa ground-slag with flour mill or pulverizer: the ratio of water 1:5~20 adds 45~85 DEG C Warm water stirs evenly.
8. the preparation method of Moringa dietary fiber as described in claim 1, which is characterized in that autoclave conditions are as follows: 115~ Sterilize 15~20min at 125 DEG C, and temperature is down to 50 DEG C or less and opens autoclave again after sterilizing.
9. a kind of Moringa dietary fiber that the preparation method as described in claim 1~8 is any obtains.
10. a kind of Moringa dietary fiber as claimed in claim 9 is preparing the application in Moringa dietary fiber lozenge, feature It is, the Moringa dietary fiber lozenge includes the ingredient of following parts by weight: 50~70 parts of Moringa dietary fiber dry powder, mangrove bark 10~20 parts of dry powder, 10~15 parts of whey powder, 0.5~1.0 part of stevioside, 0.5~3.0 part of maltodextrin, magnesium stearate 0.1~ 1.0 parts and 10~20 parts of drinkable water.
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