CN107509901A - The method for preparing flavor edible mushroom juice using leavening is compounded - Google Patents

The method for preparing flavor edible mushroom juice using leavening is compounded Download PDF

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Publication number
CN107509901A
CN107509901A CN201710699323.8A CN201710699323A CN107509901A CN 107509901 A CN107509901 A CN 107509901A CN 201710699323 A CN201710699323 A CN 201710699323A CN 107509901 A CN107509901 A CN 107509901A
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China
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leavening
edible mushroom
raw material
lactobacillus
compounding
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CN201710699323.8A
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Chinese (zh)
Inventor
纪超凡
胡静
张婧博
韩静
林心萍
梁会朋
毛晨宇
祁立波
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Dalian Polytechnic University
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Dalian Polytechnic University
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Priority to CN201710699323.8A priority Critical patent/CN107509901A/en
Publication of CN107509901A publication Critical patent/CN107509901A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/123Bulgaricus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/125Casei
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/133Curvatus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/137Delbrueckii
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/143Fermentum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/175Rhamnosus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/531Lactis

Abstract

The invention provides a kind of method for preparing flavor edible mushroom juice using leavening is compounded, concretely comprise the following steps:Compound the preparation of leavening:Enzyme preparation is mixed with lactic acid bacteria, obtains compounding leavening;Non-rot high-quality edible mushroom raw material is selected, is smashed with pulverizer and adds water, immersion;Into raw material soaking liquid, inoculation compounding leavening, is fermented;Centrifugal filtration, collect supernatant, as flavor edible mushroom juice.The compounding leavening that the present invention uses includes enzyme preparation and microorganism, and fermentation process can eliminate the wooden fishy smell of edible mushroom, and the edible mushroom juice mouthfeel after fermentation is soft, well-balanced;Enzyme preparation and lactic acid bacteria in the compounding leavening used can make polysaccharide, protein, trace element in raw material fully be discharged into liquid phase, can also degrade the composition of more difficult absorption in raw material while raw material flavor is improved, the active ingredients such as polysaccharide are made preferably to discharge, it is easier to digest and assimilate, the product is had the beneficiating ingredient of edible mushroom and the two-fold advantage of fermented product concurrently.

Description

The method for preparing flavor edible mushroom juice using leavening is compounded
Technical field
It is more particularly to a kind of to utilize the compounding being made up of enzyme preparation and lactic acid bacteria the present invention relates to fermented beverage technical field The method that leavening prepares flavor edible mushroom juice.
Background technology
High quality edible fungus is delicious, nutritious, and its protein content is higher by taking black fungus as an example and is good protein, rich Containing 8 kinds of amino acid needed by human.Both raw materials, containing several physiological active substances perhaps, have spy also rich in polysaccharide, calcium, iron etc. Different healthcare function.The edible fungi polysaccharide that particularly Recent study is found, there is anti-aging, anti-diabetic, anticancer to be particularly Anti-liver cancer and anti-and other effects, and have the medical care effect of enhancing Human Physiology activity.
China is the big country of mushroom producing such as flat mushroom, black fungus, white fungus, and traditional processing mode is based on drying, product shape Formula is single.Using edible mushroom as raw material, cold fermentation method is that to produce nutrition, flavor beverage be new think of in recent years to processing mode for exploitation Road.Harbin Mo Yi Bioisystech Co., Ltd has applied for " a kind of preparation method of black fungus lactic acid fermentation beverage " in 2014 (application number:201410705328.3);Open to ascend 2011 and applied for " preparation method of a kind of Pfansteihl agaric and its drink " (Shen Please number:201110265273.5);The application of Jilin Agriculture University 2016 " a kind of pure plant soybean white fungus fermented yoghourt and Its production method " (application number:201610441713.0).But lactic acid bacteria fermenting agent lacks corresponding destruction edible mushroom cell membrane Enzyme system so that the nutritional ingredient such as polysaccharide in edible mushroom cell fails fully to be discharged into zymotic fluid.
The present invention using a kind of compounding leavening while raw material flavor is improved the polysaccharide in agaric etc. can also seek Form and point be more effectively discharged into flavor edible mushroom juice.In addition, compounding leavening inoculation fermentation technique is simple, equipment cost Low, energy consumption is low, is easily promoted in Edible Fungi area.
The content of the invention
It is an object of the invention to provide one kind using edible mushroom as main material, fermented type flavor is prepared using composite ferment The method of edible mushroom juice;Using the composition for compounding more difficult absorption in leavening degraded raw material, make the active ingredients such as polysaccharide more preferable It is discharged into zymotic fluid.
To reach above-mentioned purpose, the invention provides a kind of using compounding the method that leavening prepares flavor edible mushroom juice, Concretely comprise the following steps:
S1, the preparation for compounding leavening:By enzyme preparation and lactic acid bacteria with mass ratio 1:1 mixing, obtain compounding leavening;
The enzyme preparation is one kind in pectase, papain, cellulase, neutral proteinase, acid protease Or a variety of mixing;
The lactic acid bacteria is Lactobacillus plantarum (Lactobacillus plantarum), newborn Bifidobacterium Bifidum (Bifidobacterium lactis), Lactobacillus casei (Lactobacillus casei), lactobacillus fermenti (Lactobacillus fermentum), Lactobacillus rhamnosus (Lactobacillus rhamnosus), lactobacillus acidophilus (Lactobacillus acidophilus), Lactobacillus delbrueckii (Lactobacillus delbrueckii), lactobacillus curvatus (Lactobacillus curvatus), streptococcus thermophilus (Streptococcus thermophilus), bulgarian milk bar One or more mixing in bacterium (Lactobacillus bulgaricus);
S2, raw material pre-treatment:Non-rot high-quality edible mushroom raw material is selected, is smashed with pulverizer, crosses 20-40 mesh Sieve, to expect water quality ratio as 1:(5-60) is into the raw material of crushing plus water, immersion 30-150 minutes, soaking temperature are 30-90 DEG C, Raw material soaking liquid is obtained, is dried in the air cool standby;
Under preferred embodiment, the edible mushroom is black fungus, Uricularia polytricha, white fungus, flat mushroom, mushroom;
S3, leavening is compounded made from inoculation step S1 into raw material soaking liquid made from step S2, fermented, obtained Fermenting raw materials stoste;
The addition of the compounding leavening is 1-15% (leavening quality/soak quality);
Under preferred embodiment, the detailed process of above-mentioned inoculation and fermentation is:Raw material soaking liquid made from step S2 is put into perseverance In warm oscillator, addition compounding leavening 1-15% (leavening quality/soak quality), be incubated 20-60 DEG C, sealing and standing or Stirring fermentation 4-16 hours, fermenation raw liquid pH terminate fermentation after being down to less than 5, obtain fermenting raw materials stoste;
S4, centrifugal filtration:Fermenting raw materials stoste made from step S3 is centrifuged, collects supernatant, as flavor edible mushroom Juice;
The centrifugal process is specially:With 2000-10000 revs/min of centrifugation 10 minutes.
After the flavor edible mushroom juice that step S4 is obtained carries out pasteurize or high temperature, more conducively store.
Compared to prior art effect and have an advantage that:
1st, invention introduces enzyme preparation, edible fungi polysaccharide can be made effectively to be discharged into stoste, promotes to absorb.
2nd, invention introduces microbe leaven, fermentation process can eliminate the wooden fishy smell of edible mushroom;It is edible after fermentation Bacterium juice mouthfeel is soft, well-balanced.
3rd, the present invention changes the tradition of raw material and eats form, and the enzyme preparation and lactic acid bacteria in the compounding leavening used can So that the polysaccharide, protein, trace element in raw material are fully discharged into liquid phase, can also be degraded while raw material flavor is improved The composition of more difficult absorption in raw material, makes the active ingredients such as polysaccharide preferably discharge, it is easier to digests and assimilates, the product is had food concurrently With the beneficiating ingredient of bacterium and the two-fold advantage of fermented product.
4th, this processing technology is simply easily promoted, equipment is simple, energy consumption is low.
Embodiment
For advantages of the present invention and purpose is more clearly understood, the technical side with reference to specific embodiment to the present invention Case elaborates, but protection scope of the present invention is not limited to that embodiment.
Embodiment 1
Non-rot high-quality black fungus is selected, is smashed with pulverizer, sieves (40 mesh), takes the black fungus 100 smashed Gram immersion, material-water ratio 1:40, soaking temperature is 90 DEG C, and soak time is 120 minutes, is dried in the air cool standby, obtains black fungus immersion Liquid.(lactobacillus bulgaricus and papain are according to 1 for access compounding leavening:1 mass ratio mix) 3% to black fungus soak Liquid, 45 DEG C of fermentation temperature, fermentation time are 12 hours.After fermentation ends, black fungus fermenation raw liquid centrifuges 10 in 4000 revs/min Minute, pasteurization, obtain flavor black fungus zymotic fluid.Its flavor and Major Nutrient index are commented (table 1), main thing Property index is shown in (table 2).Flavor black fungus juice wood fishy smell disappears after fermentation, fragrant increase, has the unique taste.In addition, black fungus is sent out Zymotic fluid improves 1.1 times to human body beneficiating ingredient, polysaccharide in fermentation liquid content rich in protein, polysaccharide, calcium, iron etc. compared with soak, 2.6 times and 1.9 times are respectively increased in calcium and iron content.
The flavor black fungus zymotic fluid Major Nutrient index of table 1.
The main physical index of flavor black fungus zymotic fluid of table 2.
Embodiment 2
Non-rot high-quality white fungus is selected, is smashed with pulverizer, is sieved (40 mesh), takes the 75 grams of leachings of white fungus smashed Bubble, material-water ratio 1:50, soaking temperature is 80 DEG C, and soak time is 60 minutes, dries in the air cool standby, obtains white fungus soak.Access Compounding leavening, (streptococcus thermophilus, Lactobacillus plantarum and cellulase are according to 1:1:2 mass ratioes mix) 2.5% to white fungus soak Liquid, 45 DEG C of hydrolysis temperature, fermentation time are 12 hours.After fermentation ends, white fungus fermenation raw liquid centrifuges 10 points in 4000 revs/min Clock, pasteurization, obtain flavor white fungus zymotic fluid.Its flavor and Major Nutrient index are commented (table 3), main physical property refers to Mark is shown in (table 4).Flavor tremella juice taste is pure and fresh after fermentation, has the unique taste.It is beneficial to human body rich in protein, polysaccharide, calcium, iron etc. Composition, after compounding is ferment-fermented, polysaccharide in fermentation liquid content improves 0.8 times compared with soak.
The flavor white fungus zymotic fluid leading indicator of table 3.
The main physical index of flavor silver fungus fermentation liquid of table 4.
Product index Index details
Zero shear viscosity characterization 19.8P.s
Zeta potential -49mV
Average particle size distribution 253nm
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto, Any one skilled in the art in the technical scope of present disclosure, technique according to the invention scheme and its Inventive concept is subject to equivalent substitution or change, should all be included within the scope of the present invention.

Claims (4)

1. a kind of utilize compounds the method that leavening prepares flavor edible mushroom juice, it is characterised in that concretely comprises the following steps:
S1, the preparation for compounding leavening:By enzyme preparation and lactic acid bacteria with mass ratio 1:1 mixing, obtain compounding leavening;
The enzyme preparation is pectase, papain, cellulase, neutral proteinase, one kind in acid protease or more Kind mixing;
The lactic acid bacteria is Lactobacillus plantarum, newborn Bifidobacterium Bifidum, Lactobacillus casei, lactobacillus fermenti, Lactobacillus rhamnosus, acidophilus In lactobacillus, Lactobacillus delbrueckii, lactobacillus curvatus, one or more mixing of streptococcus thermophilus, lactobacillus bulgaricus;
S2, raw material pre-treatment:Non-rot high-quality edible mushroom raw material is selected, is smashed with pulverizer, crosses 20-40 mesh sieves, with It is 1 to expect water quality ratio:(5-60) is into the raw material of crushing plus water, immersion 30-150 minutes, soaking temperature are 30-90 DEG C, obtains Raw material soaking liquid, dry in the air cool standby;
S3, leavening is compounded made from inoculation step S1 into raw material soaking liquid made from step S2, fermented, obtain raw material Fermenation raw liquid;
The addition of the compounding leavening is 1-15% (leavening quality/soak quality);
S4, centrifugal filtration:Fermenting raw materials stoste made from step S3 is centrifuged, collects supernatant, as flavor edible mushroom juice.
2. the method for preparing flavor edible mushroom juice using leavening is compounded according to claim 1, it is characterised in that step S1 The edible mushroom is black fungus, Uricularia polytricha, white fungus, flat mushroom, mushroom.
3. the method for preparing flavor edible mushroom juice using leavening is compounded according to claim 1, it is characterised in that step S2 The inoculation and the detailed process fermented are:Raw material soaking liquid made from step S2 is put into constant temperature oscillator, addition compounding Leavening 1-15% (leavening quality/soak quality), 20-60 DEG C is incubated, sealing and standing or stirring are fermented 4-16 hours, hair Ferment stoste pH terminates fermentation after being down to less than 5, obtains fermenting raw materials stoste.
4. the method for preparing flavor edible mushroom juice using leavening is compounded according to claim 1, it is characterised in that step S4 The centrifugal process is specially:With 2000-10000 revs/min of centrifugation 10 minutes.
CN201710699323.8A 2017-08-16 2017-08-16 The method for preparing flavor edible mushroom juice using leavening is compounded Pending CN107509901A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110923171A (en) * 2019-12-20 2020-03-27 黑河君行生物科技有限责任公司 Lactic acid bacteria for removing earthy taste of black fungus and enzyme preparation method thereof
CN111096405A (en) * 2020-01-15 2020-05-05 安徽科技学院 Preparation method of puffed corn-flavor fermented beverage
CN111134199A (en) * 2020-02-20 2020-05-12 杭州九阳豆业有限公司 Preparation method of beany flavor-free fermented soybean milk and prepared beany flavor-free fermented soybean milk
WO2020177421A1 (en) * 2019-03-01 2020-09-10 江南大学 Method for preparing cordyceps militaris ferment by two-stage fermentation and complex enzymatic hydrolysis
CN112971118A (en) * 2021-03-03 2021-06-18 陕西三八妇乐特医食品有限公司 Preparation method of black fungus fermented nutritional product
CN115595332A (en) * 2022-07-28 2023-01-13 广东植雅世家生物科技有限公司(Cn) Method for preparing tremella fermentation liquor by combining compound enzyme with microbial fermentation
CN115644423A (en) * 2022-10-27 2023-01-31 西北农林科技大学 Probiotic fermented shiitake juice with immunoregulation activity

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CN105533351A (en) * 2016-01-08 2016-05-04 黑龙江省聚拢乾坤农业技术开发有限公司 Black fungus health-care beverage and production technology thereof
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Cited By (9)

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Publication number Priority date Publication date Assignee Title
WO2020177421A1 (en) * 2019-03-01 2020-09-10 江南大学 Method for preparing cordyceps militaris ferment by two-stage fermentation and complex enzymatic hydrolysis
CN110923171A (en) * 2019-12-20 2020-03-27 黑河君行生物科技有限责任公司 Lactic acid bacteria for removing earthy taste of black fungus and enzyme preparation method thereof
CN110923171B (en) * 2019-12-20 2021-07-27 黑河君行生物科技有限责任公司 Lactic acid bacteria for removing earthy taste of black fungus and enzyme preparation method thereof
CN111096405A (en) * 2020-01-15 2020-05-05 安徽科技学院 Preparation method of puffed corn-flavor fermented beverage
CN111134199A (en) * 2020-02-20 2020-05-12 杭州九阳豆业有限公司 Preparation method of beany flavor-free fermented soybean milk and prepared beany flavor-free fermented soybean milk
CN112971118A (en) * 2021-03-03 2021-06-18 陕西三八妇乐特医食品有限公司 Preparation method of black fungus fermented nutritional product
CN112971118B (en) * 2021-03-03 2022-09-06 陕西三八妇乐特医食品有限公司 Preparation method of black fungus fermented nutritional product
CN115595332A (en) * 2022-07-28 2023-01-13 广东植雅世家生物科技有限公司(Cn) Method for preparing tremella fermentation liquor by combining compound enzyme with microbial fermentation
CN115644423A (en) * 2022-10-27 2023-01-31 西北农林科技大学 Probiotic fermented shiitake juice with immunoregulation activity

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