CN101524150B - Microbial ecological agent and preparation method thereof - Google Patents
Microbial ecological agent and preparation method thereof Download PDFInfo
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- CN101524150B CN101524150B CN200910038538A CN200910038538A CN101524150B CN 101524150 B CN101524150 B CN 101524150B CN 200910038538 A CN200910038538 A CN 200910038538A CN 200910038538 A CN200910038538 A CN 200910038538A CN 101524150 B CN101524150 B CN 101524150B
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Abstract
The invention discloses a microbial ecological agent and a preparation method thereof. The microbial ecological agent mainly consists of the following raw materials based on the parts by weight: 5-50 of north caterpillar fungus reactants, 20-80 of lactic acid bacteria freeze-dried power, 5-20 of bifidus factors and 0.2-5 of vitamin; the north caterpillar fungus reactants contain 100g of north caterpillar fungus extract, 0.1-3g of alanine, 0.5-10g of glucose, 0.01-0.5g of ascorbic acid, 0-8g of sault, 0-2g of I+G and 0.001-0.01g of BHT. The microbial ecological agent is formed by preparing the north caterpillar fungus reactants, the lactic acid bacteria freeze-dried power and then mixing all the components, has multiple functions of digestion assistance, gastrointestinal disease prevention and cure, micro ecological balance maintenance and special health-care function of the north caterpillar fungus, and the like and has unique flavor and good taste. The preparation process is simple, the modern fermentation process is adopted, the north caterpillar fungus extract containing rich nutrition of amino acid, trace elements, and the like are added to fermentate and cultivate probiotics, and the number of fermentation liquid live bacteria is improved.
Description
Technical field
The invention belongs to biotechnology, relate to a kind of probiotics and preparation method thereof specifically.
Background technology
The north Chinese caterpillar fungus is also claimed Cordyceps militaris, is the abbreviation of northern Chinese caterpillar Fungus, has another name called northern Chinese caterpillar Fungus, silkworm chrysalis Cordyceps sinensis etc.After the nineties in 20th century, many scholars have carried out big quantity research to domestication and the pharmacological action of northern Chinese caterpillar fungus, confirm that the pharmacological action and the effect of north grass is identical with Cordyceps sinensis basically, can be used as the substitute of Cordyceps sinensis.Lung nourishing and qi invigorating arranged, increase smart kidney tonifying, hemostasis and phlegm, inhibition tumour, cough-relieving calmness, wind dispelling, heat radiation, stay Rong Meiyan, delay senility, tonifying kidney and strengthening yang; The reduction youth; Effect and lung moistening and asthma relievings such as harmonious two sexual lifves, the enriching yin tonifying lung enhances metabolism; Improve the health, improve the effect of immunity of organisms.Can be widely used in industries such as bio-pharmaceuticals, effect type health products, cosmetics, flavouring.The northern Chinese caterpillar Fungus health products that gone on the market at present have: the aweto capsule of cordyceps militaris oral liquid, Chinese caterpillar fungus kidney-tonifying wine, Chinese caterpillar fungus health beer and Agricultural University Of Shenyang's development etc.Though the product of some northern Chinese caterpillar Fungus is arranged on the market, these product majorities rest on the roughing level, and therefore lacking of market competition ability, guarantee the development that the northern Chinese caterpillar Fungus industry can be continual and steady, just must improve the scientific and technological content of its converted products.In addition, what deserves to be mentioned is that in recent years, along with efficacy study and propaganda to northern Chinese caterpillar Fungus deepen continuously, foreign vendor had also produced keen interest to it, export volume constantly increases.And countries such as Japan and Korea S are in recent years all in the research and development of strengthening the Chinese caterpillar fungus functional food.
Along with the raising of living standards of the people and the enhancing of health perception, people more and more pay close attention to diet to organism physiology or otherwise influence and sanatory effect, and the propensity to consume is just turning to the food with adequate nutrition and health care.Because probio has good regulating action to body intestinal cording, enteron aisle fungus strain balance then is to be related to human nutrition and healthy vital factor, and this makes the research of probio become the focus in the functional food research gradually.Lactic acid bacteria is an important physical property probio in the human body intestinal canal, has invasion and the effects such as infection and enhancing body immunity keeping the normal microecological balance of intestines and stomach, suppress pathogen, is the main little ecological type raw material of plurality kinds of health care food and medicine.Commercial product has: sour milk, lactic acid bacteria tablet, lactic acid bacteria powder etc.Mei Shi in 1882 finds lactic acid bacteria, and day in 1971, the Benson factory that suckles forever produced the bifid sour milk, and beginning is sold in the European market.French ST.Hubert company in 1986, the Benson factory that suckles forever introduces production technology from day, and bifidobacterium yoghurt has a large circulation freely.20th century the eighties, China begins to produce.Oilgosaccharkdes is meant that oligosaccharides etc. is not digested and absorbs by the digestive ferment in the human body alimentary canal; But, it can promote the growth of beneficial bacteriums such as Bifidobacterium in the enteron aisle after getting into human body intestinal canal selectively; Keep the general level of the health of human body, commercial product has: FOS, soyabean oligosaccharides, palatinose-oligosaccharides, oligoisomaltose etc.But do not see yet and utilize northern Cordyceps to promote lactobacillus-fermented, improve the zymotic fluid viable count, and northern Chinese caterpillar fungus and biodiasmin are organically combined the report of developing the special health care compound health products of both.
According to searching document and database, detecting the document relevant with the present invention has:
1, the research of northern Chinese caterpillar fungus commercialization cultivation technique (profound scholar friend etc., 2008).
2, the discarded compost Polysaccharide from Bee optimization research of northern Chinese caterpillar fungus (Ren Shuyu etc.; 2008), a large amount of discarded compost that the Chinese caterpillar fungus artificial cultivation produces adopts orthogonal design; Confirm to be: extract 80 ℃ of temperature from the condition of extracting polysaccharide; Extract extraction time 120min, solid-liquid ratio (w/v) 1: 20 2 times.Cleaner production and utilize resources synthetically that this experimental result can be northern Chinese caterpillar fungus provide reference.
3, active ingredient compares (Yang Qing etc. in the northern Chinese caterpillar fungus powder of two kinds of processing method acquisitions; 2008); Purpose has detected cordycepin in the Chinese caterpillar fungus dry powder, thick polysaccharide, amino acid and vitamin equal size for the active ingredient of the northern Chinese caterpillar fungus dry powder of two kinds of processing methods acquisitions of clear and definite freeze-drying and oven dry.
4, the research and development of northern Chinese caterpillar fungus and active component thereof (Ni He etc., 2007).
5, northern Chinese caterpillar fungus batch production high-yield culture technique (Zhao Zhanchun etc., 2006).
6, compound north aweto capsule quality standard is inquired into (Liu Weimin etc., 2000).
7, the present Research of northern Chinese caterpillar fungus (Ge Beilei etc., 1999).
8, the research of northern Chinese caterpillar fungus resistant activity oxygen radical effect (Shen Qiying etc., 1997).
In document and the database retrieval scope, searching document has been introduced relevant northern Chinese caterpillar fungus at home.Mention the utilization of relevant cultivation, analysis of components, extract etc.But do not mention local flavorization.
Though northern Chinese caterpillar fungus, lactic acid bacteria and Oilgosaccharkdes be commercialization production and sales all, the function of these goods is all more single, and much the mouthfeel and the local flavor of health products do not obtain paying attention to.
Summary of the invention
One of the object of the invention provides a kind of probiotics, and this probiotics has plurality of health care functions, also has good mouthfeel and local flavor simultaneously.
The technical scheme that realizes above-mentioned purpose is following:
A kind of probiotics, it mainly is made up of the raw material of following weight portion:
North Chinese caterpillar fungus reactant 5-50 part; Lactic acid bacteria freeze drying powder 20-80 part; Oilgosaccharkdes 5-20 part; And vitamin 0.2-5 part, said northern Chinese caterpillar fungus reactant consist of 100g north Cordyceps, alanine 0.1-3g, glucose 0.5-10g, ascorbic acid 0.01-0.5g, salt 0-8g, I+G (flavor enhancement) 0-2g and BHT (anti-oxidant) 0.001-0.01g.
The preferred group of said northern Chinese caterpillar fungus reactant becomes: northern Cordyceps 100g, alanine 2g, glucose 5g, ascorbic acid 0.02g, salt 5g, I+G0.5g and BHT0.002g.
Said northern Cordyceps is to carry out being concentrated into 5-20Brix (hundred sharp degree, i.e. solubility solid content) behind the enzymolysis through hydrolysis, the alkali protease that adds northern Chinese caterpillar fungus dry product quality 0.01-0.5% to obtain.More preferably, the alkali protease that adds northern Chinese caterpillar fungus dry product quality 0.02-0.2%.
Said Oilgosaccharkdes is one or more in trehalose, FOS, oligoisomaltose, soyabean oligosaccharides, palatinose-oligosaccharides, xylo-oligosaccharide, the oligomeric maltose.
The lactic acid bacteria freeze drying powder is through fermentation, centrifugal collections lactic acid bacteria mud in the zymotic fluid that in per 100 liters of conventional culture mediums, is added with 0.1-5kg north Cordyceps, add the protective agent freeze drying then and must.Preferably, be added with 0.5-2kg north Cordyceps in per 100 liters of conventional culture mediums.
Another object of the present invention provides a kind of method for preparing above-mentioned probiotics.
A kind of method for preparing probiotics may further comprise the steps:
(1) preparation of northern Chinese caterpillar fungus reactant:
(1) adds its quality 5-40 water doubly in the Chinese caterpillar fungus dry product northwards, smash pulping after boiling, at 70-98 ℃ of following hydrolysis 1-6 hour; The pH of hydrolyzate is adjusted into 7.5-10, adds the alkali protease of northern Chinese caterpillar fungus dry product quality 0.01-0.5%, at 40-75 ℃ of following enzymolysis 4-12 hour; Filtration, vacuum are concentrated into 5-20Brix (hundred sharp degree, i.e. solubility solid content), promptly get northern Cordyceps;
(2) in the thickened pulp of every 100ml, add alanine 0.1-3g, glucose 0.5-10g, ascorbic acid 0.01-0.5g, salt 0-8g, I+G 0-2g, BHT0.001-0.01g; Mix the back and give birth to fragrant reaction 3-8 hour at 60-100 ℃; Adjustment total solid concentration is 10% spray-drying, get final product northern Chinese caterpillar fungus reactant;
(2) preparation of lactic acid bacteria freeze drying powder:
(3) cultivate lactic acid bacteria to three grade fermemtation seed; Said lactic acid bacteria culturers be in bifidobacterium bifidum, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium adolescentis, lactobacillus bulgaricus, lactobacillus acidophilus, Lactobacillus casei cheese subspecies, streptococcus thermophilus, the lactobacillus reuteri more than 2 kinds or 2 kinds;
(4) in conventional fermentation lactic acid bacterium culture medium, by adding 0.1-5kg north Cordyceps in per 100 liters of conventional culture mediums, accent pH is 5.0-7.5, sterilizes 15-40 minute for 100-121 ℃; When treating that culture medium is chilled to 10-35 ℃, three grades of seeds of every kind of lactic acid bacteria are pressed the inoculum concentration inoculation of the 2-20% (v/v) of culture medium cumulative volume, and single strain is lived or closed inoculation;
(5) technological condition for fermentation is: temperature 10-45 ℃, mixing speed 5-100r/min, stuffiness or anaerobism, tank pressure 0.01-0.10MPa, pH value maintain 5.0-7.0, time 8-24 hour; Refrigerated centrifuge is collected thalline after the fermentation ends;
(6) add the lactic acid bacteria protective agent by bacterium mud total amount, protective agent consists of: the 1-20% (w/w) of 5-20% (w/w) skimmed milk power of bacterium mud total amount, 2-10% (w/w) glycerine of bacterium mud total amount and bacterium mud total amount is selected from one or more materials in trehalose, FOS, oligoisomaltose, soyabean oligosaccharides, the palatinose-oligosaccharides; Bacterium mud behind the adding freeze drying protectant is mixed, carry out freeze drying then and get freeze-dried powder;
(3) product preparation
(7) northern Chinese caterpillar fungus reactant 5-50 part of weight portion; Dry powder of lactic acid bacteria 20-80 part, Oilgosaccharkdes 5-20 part, and vitamin 0.2-5 part (like B family vitamin, vitamin C, vitamin D, vitamin E etc.) weighing; Abundant mixing in Hi-mixer promptly gets said microecological health-care food then.
The present invention has the following advantages:
1. technology is simple; The warm plurality of health care functions of plurality kinds of health care article;
2. utilize nutritious northern Cordyceps of advanced enzyme engineering technology and Maillard reaction technology development and northern Chinese caterpillar fungus reactant with rich flavor;
3. through living fragrant reaction for preparation north Chinese caterpillar fungus reactant the time, utilize malic acid to make the product taste softer as the thermal response catalyst; Add palatinose and carry out thermal response, make the more mellow nature of northern Chinese caterpillar fungus local flavor as reduced sugar.
4. adopt modern zymotechnique, interpolation contains nutritious northern Cordyceps fermented and cultured probios such as amino acid, trace element, improves the zymotic fluid viable count;
5. utilize protease and give birth to fragrant reaction and develop northern Chinese caterpillar fungus reactant, then it is compositely gone into product, give good taste of product and nutrition;
6. go into the propagation that can promote beneficial bacterium in the enteron aisle in these health products on the one hand with Oilgosaccharkdes is compound, can promote the field planting of lactic acid bacteria in enteron aisle in these health products on the other hand.
7, adopt the scientific allocation technology, add nutriments such as northern Chinese caterpillar fungus thermal response thing, Oilgosaccharkdes, modulate unique flavor, good mouthfeel, nutritious, efficient probio active bacteria formulation with plurality of health care functions.
The specific embodiment
Embodiment one:
(1) northern Chinese caterpillar fungus reactant preparation:
1. the preparation of northern Cordyceps: get 2000 grams and do northern Chinese caterpillar fungus, add 40 premium on currency, pour beater into after boiling, pulled an oar 5 minutes, transfer pH to 9.5-10,95 ℃ of following hydrolysis 6 hours; The pH of hydrolysis slurry is adjusted into 8.5, adds the alkali protease of 4g, 60 ℃ of following enzymolysis 5 hours; It is northern Cordyceps that filtration, vacuum are concentrated into 10Brix;
2. northern Chinese caterpillar fungus reactant: in the northern Cordyceps that concentrates of every 100ml, add alanine 0.6g, glucose 3g, ascorbic acid 0.02g, BHT0.002g; Mix the back and give birth to fragrant reaction 5 hours at 85 ℃; Adjustment total solid concentration is 10% spray-drying, gets final product.
(2) preparation of active lactic acid bacteria preparation
1. lactobacillus acidophilus, lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium adolescentis are carried out actication of culture to three grade kind, culture medium is conventional lactic acid bacteria culture medium, and cultural method is conventional lactic acid bacteria cultivation;
2 with four 50 l fermentor were of four kinds of bacteria for fermentation broth per canned 30-35 liters per 100 liters of broth was composed of: tomato juice, 8 liters, Shi peptone 0.5kg, glucose 2kg, yeast extract 0.5kg, beef extract, 1.0kg, sodium acetate, 0.3kg, dipotassium hydrogen phosphate, 200g, cysteine 20g, stir after adding 1kg Cordyceps extract, after mixing with sodium hydroxide or hydrochloric acid to pH 6.5, washed with water volume to 100 liters.In 115 ℃ of sterilizations 20 minutes, be chilled to 35 ℃, inoculation fermentation;
3. lactobacillus acidophilus, lactobacillus bulgaricus, Lactobacillus plantarum are inoculated respectively by 10% (v/v) inoculum concentration of its zymotic fluid cumulative volume (culture medium cumulative volume) respectively and carried out the stuffiness fermentation, tank pressure is kept with filtrated air; Bifidobacterium adolescentis carries out anaerobic fermentation (air in jar replaces with aseptic purity nitrogen) by 15% (v/v) inoculum concentration inoculation of zymotic fluid cumulative volume;
4. fermentation parameter: tank pressure 0.03MPa, mixing speed 30r/min, 37 ℃ of temperature, pH5.5-6.5; Fermentation time lactobacillus acidophilus, lactobacillus bulgaricus, Lactobacillus plantarum are 15 hours, and the bifidobacterium adolescentis fermentation time is 20 hours;
5. distinguish four kinds of thalline of centrifugal collection, the percentage by weight (w/w) of pressing bacterium mud total amount adds: 10% skimmed milk power, trehalose 8%, soyabean oligosaccharides 10%, glycerine 1%, mixing;
6. the thalline collected of freeze drying respectively, and grind sieve four kinds of pure lactic acid bacteria freeze drying bacterium powder.
(3) product preparation
1. by the weighing of following prescription: northern Chinese caterpillar fungus reactant 1000g, lactobacillus acidophilus bacterium powder 500g, streptococcus thermophilus bacterium powder 400g, lactobacillus bulgaricus bacterium powder 400g, bifidobacterium adolescentis bacterium powder 100g, oligoisomaltose 100g, FOS 50g, palatinose-oligosaccharides 50g, vitamin C 20g, vitamin E 10g;
2. with compound abundant mixing in the double helix Hi-mixer;
3. vacuum nitrogen is packed as finished product.
Embodiment two:
(1) northern Chinese caterpillar fungus reactant preparation:
1. the preparation of northern Cordyceps: get 10kg and do northern Chinese caterpillar fungus, add 250 premium on currency, pour beater into after boiling, pulled an oar 5 minutes, transfer pH to 9.5-10,85 ℃ of following hydrolysis 3 hours; The pH of hydrolysis slurry is adjusted into 8.5, adds the flavor protease of 15g, 60 ℃ of following enzymolysis 6 hours; It is northern Cordyceps that filtration, vacuum are concentrated into 12Brix;
2. northern Chinese caterpillar fungus reactant: in the northern Cordyceps of every 100g, add alanine 1.5g, glucose 5g, monosodium glutamate 3g, salt 2g, ascorbic acid 0.02g, BHT0.002g; Mix the back and give birth to fragrant reaction 6 hours at 90 ℃; Adjustment total solid concentration is 10% spray-drying, gets final product.
(2) preparation of active lactic acid bacteria preparation
1. lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium infantis and bifidobacterium adolescentis are carried out actication of culture to three grade kind, culture medium is conventional lactic acid bacteria culture medium, and cultural method is conventional lactic acid bacteria cultivation;
2 with two 500-liter fermenter fermentation, fermentation tank No. 1 and No. 2, each canned broth 350-400 liters per 100 liters of broth was composed of: tomato juice, 8 liters, Shi peptone 0.5kg, glucose 2kg, yeast extract 0.5kg, beef extract 1.0kg, sodium acetate 0.3kg, dipotassium hydrogen phosphate 200g, cysteine 20g, stir after adding 2kg Cordyceps extract, after mixing with sodium hydroxide or hydrochloric acid to adjust pH to 6.5, at 115 ℃ sterilized for 20 minutes, cooled to 35 ℃, to be vaccinated;
3. three grades of kinds of lactobacillus bulgaricus and streptococcus thermophilus are inoculated in No. 1 fermentation tank in 1: 1 ratio, total inoculum concentration is 10% (v/v) of zymotic fluid cumulative volume, adopts the stuffiness fermented and cultured, and tank pressure is kept with filtrated air;
4. three grades of kinds of bifidobacterium infantis and bifidobacterium adolescentis are inoculated in No. 2 fermentation tanks in 1: 1 ratio, total inoculum concentration is 15% (v/v) of zymotic fluid cumulative volume, adopts anaerobism to cultivate, and the air in jar replaces with aseptic purity nitrogen;
5. technological condition for fermentation: tank pressure 0.03MPa, mixing speed 50r/min, 37 ℃ of temperature, pH5.5-6.5,15 hours, No. 2 jar fermentation times of No. 1 jar fermentation time are 20 hours;
6. the thalline of difference No. 1 jar of centrifugal collection and No. 2 jars after the mixing of bacterium mud, is pressed percentage by weight (w/v) adding of bacterium mud total amount: skimmed milk power 10%, trehalose 10%, FOS 10%, glycerine 2%, mixing;
7. freeze drying thalline, and grind sieve compound freeze-dried lactic acid bacteria powder.
(3) product preparation
1. by the weighing of following prescription: northern Chinese caterpillar fungus reactant 2kg, compound lactobacillus freeze-dried powder 5kg, FOS 0.2kg, soyabean oligosaccharides 0.1kg, oligoisomaltose 0.3kg, palatinose-oligosaccharides 0.2kg, xylo-oligosaccharide 0.2kg, vitamin C 0.2kg, vitamin D 0.1kg, B family vitamin 0.2kg;
2. with compound abundant mixing in the double helix Hi-mixer;
3. vacuum nitrogen is packed as finished product.
Embodiment three:
(1) northern Chinese caterpillar fungus reactant preparation:
1. the preparation of northern Cordyceps: get 20kg and do northern Chinese caterpillar fungus, add 300 premium on currency, pour beater into after boiling, pulled an oar 5 minutes, transfer pH to 9.5-10,75 ℃ of following hydrolysis 4 hours; The pH of hydrolysis slurry is adjusted into 8.5, adds the alkali protease of 30g, 60 ℃ of following enzymolysis 5 hours; Filtration, vacuum are concentrated into 16Brix, promptly northern Cordyceps;
2. northern Chinese caterpillar fungus reactant: in the northern Cordyceps of every 100g, add alanine 0.8g, glucose 3g, monosodium glutamate 1g, salt 1.5g, ascorbic acid 0.02g, BHT0.002g; Mix the back and give birth to fragrant reaction 7 hours at 75 ℃; Adjustment total solid concentration is 10% spray-drying, gets final product.
(2) preparation of active lactic acid bacteria preparation
1. lactobacillus bulgaricus, streptococcus thermophilus, lactobacillus reuteri are carried out actication of culture to three grade kind, culture medium is conventional lactic acid bacteria culture medium, and cultural method is conventional lactic acid bacteria cultivation;
2 cans of 1000 liters of broth broth 750-800 liters per 100 liters of broth was composed of: tomato juice, 8 liters, Shi peptone 0.5kg, glucose 2kg, yeast extract 0.5kg, beef extract, 1.0kg, 0.3kg sodium acetate , dipotassium hydrogen phosphate 200g, cysteine 20g, stir after adding 4kg Cordyceps extract, after mixing with sodium hydroxide or hydrochloric acid to adjust pH to 6.5, at 115 ℃ sterilized for 20 minutes, cooled to 35 ℃, to be vaccinated;
3. when being chilled to 38 ℃ lactobacillus bulgaricus, streptococcus thermophilus, three grades of kinds of lactobacillus reuteri are inoculated in 1000 liters of fermentation tanks in 1: 1: 1 ratio; Total inoculum concentration is 10% (v/v) of zymotic fluid cumulative volume; Adopt the stuffiness fermented and cultured, tank pressure is kept with filtrated air;
4. technological condition for fermentation: tank pressure 0.03MPa, mixing speed 15r/min, 37 ℃ of temperature, pH5.5-6.5, fermentation time 18 hours;
5. centrifugal collection thalline, the percentage by weight (w/w) of pressing bacterium mud total amount adds: skimmed milk power 10%, trehalose 3%, oligoisomaltose 5%, glycerine 5%, mixing;
6. freeze drying thalline, and grind sieve the compound lactobacillus freeze-dried powder.
(3) product preparation
1. by the weighing of following prescription: northern Chinese caterpillar fungus reactant 3kg, compound lactobacillus freeze-dried powder 5kg, FOS 0.5kg, soyabean oligosaccharides 0.2kg, oligoisomaltose 0.2kg, palatinose-oligosaccharides 1kg, vitamin C 0.3kg, B family vitamin 0.1kg;
2. with compound abundant mixing in the double helix Hi-mixer;
3. vacuum nitrogen is packed as finished product.
Techno-economic effect of the present invention:
It is active microbiological prepns that the present invention produces product, and its index detects with reference to lactic acid bacteria standard QB1554-1992 " sour milk beverage " and GB16321-2003 " sour milk beverage sanitary standard ".Each item index result such as the following table of product:
Table 1 sense index
| Project | Index |
| Tissue morphology | Powdery |
| Color and luster | Be yellow to orange-yellow |
| Smell and flavour | Have the intrinsic fragrance of northern Chinese caterpillar fungus and characteristic flavor on basis, free from extraneous odour and stink |
| Impurity | The visible impurity of no naked eyes |
1.1 physical and chemical index
Should meet table 2 regulation.
Table 2 physical and chemical index
| Project | Index |
| Total arsenic (in As), mg/kg | ≤0.5 |
| Plumbous (in Pb), mg/kg | ≤1 |
| Food additives | Should meet GB 2760 regulations |
1.2 microbiological indicator
Meet table 3 requirement
Table 3 microbiological indicator
This product reaches the requirement of active bacteria formulation through the check viable count.Best in quality, the raciness of local flavor active microbiological prepns of the present invention's development, the product tool has the health care of northern Chinese caterpillar fungus and lactic acid bacteria concurrently, possesses the special bright fragrance with northern Chinese caterpillar fungus again simultaneously, belongs to high-grade health food.Research and develop this product good exemplary role is played in the development of the industry, with the development that promotes the functional food industry.
Claims (5)
1. a probiotics is characterized in that, it is made up of the raw material of following weight portion:
North Chinese caterpillar fungus reactant 5-50 part; Lactic acid bacteria freeze drying powder 20-80 part; Oilgosaccharkdes 5-20 part; And vitamin 0.2-5 part, the consisting of of said northern Chinese caterpillar fungus reactant: northern Cordyceps 100g, alanine 0.1-3g, glucose 0.5-10g, ascorbic acid 0.01-0.5g, salt 0-8g, I+G 0-2g and BHT0.001-0.01g;
Said northern Cordyceps is to carry out being concentrated into 5-20Brix behind the enzymolysis through hydrolysis, the alkali protease that adds northern Chinese caterpillar fungus dry product quality 0.01-0.5% to obtain; In the northern Cordyceps thickened pulp of every 100g, add alanine 0.1-3g, glucose 0.5-10g, ascorbic acid 0.01-0.5g, salt 0-8g, I+G 0-2g, BHT0.001-0.01g; Mix the back and give birth to fragrant reaction 3-10 hour at 60-100 ℃; Adjustment total solid concentration is 10% spray-drying, get final product northern Chinese caterpillar fungus reactant;
Said lactic acid bacteria freeze drying powder is that 0.1-5kg north Cordyceps ferments, centrifugal collections lactic acid bacteria mud through in per 100 liters of fermentation mediums, being added with, add the protective agent freeze drying then and must;
Said lactic acid bacteria culturers be in bifidobacterium bifidum, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium adolescentis, lactobacillus bulgaricus, lactobacillus acidophilus, Lactobacillus casei cheese subspecies, streptococcus thermophilus, the lactobacillus reuteri more than 2 kinds or 2 kinds.
2. probiotics according to claim 1 is characterized in that, said Oilgosaccharkdes is one or more in trehalose, FOS, oligoisomaltose, soyabean oligosaccharides, palatinose-oligosaccharides, xylo-oligosaccharide, the oligomeric maltose.
3. a method for preparing probiotics is characterized in that, may further comprise the steps:
(1) preparation of northern Chinese caterpillar fungus reactant:
(1) adds its quality 5-40 water doubly in the Chinese caterpillar fungus dry product northwards, smash pulping after boiling, at 70-98 ℃ of following hydrolysis 1-6 hour; The pH of hydrolyzate is adjusted into 7.5-10, adds the alkali protease of northern Chinese caterpillar fungus dry product quality 0.01-0.5%, at 40-75 ℃ of following enzymolysis 4-12 hour; Filtration, vacuum are concentrated into 5-20Brix, promptly get northern Cordyceps;
(2) in the northern Cordyceps thickened pulp of every 100g, add alanine 0.1-3g, glucose 0.5-10g, ascorbic acid 0.01-0.5g, salt 0-8g, I+G 0-2g, BHT0.001-0.01g; Mix the back and give birth to fragrant reaction 3-10 hour at 60-100 ℃; Adjustment total solid concentration is 10% spray-drying, get final product northern Chinese caterpillar fungus reactant;
(2) preparation of lactic acid bacteria freeze drying powder:
(3) cultivate lactic acid bacteria to three grade fermemtation seed; Said lactic acid bacteria culturers be in bifidobacterium bifidum, bifidobacterium infantis, bifidobacterium longum, bifidobacterium breve, bifidobacterium adolescentis, lactobacillus bulgaricus, lactobacillus acidophilus, Lactobacillus casei cheese subspecies, streptococcus thermophilus, the lactobacillus reuteri more than 2 kinds or 2 kinds;
(4) in conventional fermentation lactic acid bacterium culture medium, by adding 0.1-5kg north Cordyceps in per 100 liters of conventional culture mediums, accent pH is 5.0-7.5, sterilizes 15-40 minute for 100-121 ℃; When treating that culture medium is chilled to 10-35 ℃, three grades of seeds of every kind of lactic acid bacteria are pressed the inoculum concentration inoculation of the 2-20% of culture medium cumulative volume, single strain or close inoculation;
(5) technological condition for fermentation is: temperature 10-45 ℃, mixing speed 5-100r/min, stuffiness or anaerobism, tank pressure 0.01-0.10MPa, pH value maintain 5.0-7.0, time 8-24 hour; Refrigerated centrifuge is collected bacterium mud after the fermentation ends;
(6) add the lactic acid bacteria protective agent by bacterium mud total amount, protective agent consists of: the 1-10wt% glycerine of the 5-20wt% skimmed milk power of bacterium mud total amount, bacterium mud total amount and the 1-20wt% of bacterium mud total amount are selected from one or more materials in trehalose, FOS, oligoisomaltose, soyabean oligosaccharides, the palatinose-oligosaccharides; Bacterium mud behind the adding freeze drying protectant is mixed, carry out freeze drying then and get freeze-dried powder;
(3) product preparation
(7) northern Chinese caterpillar fungus reactant 5-50 part of weight portion, lactic acid bacteria freeze drying powder 20-80 part, Oilgosaccharkdes 5-20 part, and vitamin 0.2-5 part, weighing, abundant then mixing promptly gets said probiotics.
4. the method for preparing probiotics according to claim 3 is characterized in that, in the step (1), the quality that adds alkali protease is the 0.02-0.2% of northern Chinese caterpillar fungus dry product quality.
5. the method for preparing probiotics according to claim 3 is characterized in that, in the step (4), by adding 0.5-2kg north Cordyceps in per 100 liters of conventional culture mediums.
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| CN200910038538A CN101524150B (en) | 2009-04-10 | 2009-04-10 | Microbial ecological agent and preparation method thereof |
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| CN200910038538A CN101524150B (en) | 2009-04-10 | 2009-04-10 | Microbial ecological agent and preparation method thereof |
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| CN101731511B (en) * | 2010-02-03 | 2013-04-03 | 徐赟姣 | Probiotic active product and preparation method thereof |
| CN102660529A (en) * | 2012-05-16 | 2012-09-12 | 苏州先阔生物科技有限公司 | Pepsin intensifier, pepsin composition and application of pepsin intensifier |
| CN102660530A (en) * | 2012-05-16 | 2012-09-12 | 苏州先阔生物科技有限公司 | Alkali protease composition with enhanced activity, and application thereof |
| CN104498360B (en) * | 2014-12-15 | 2017-08-22 | 广东省微生物研究所 | A kind of lactobacillus paracasei active bacteria formulation normal-temperature vacuum drying protectant and its application process |
| CN105602875B (en) * | 2016-03-30 | 2018-06-19 | 广州科盛生物科技有限公司 | A kind of preparation method of lactic acid bacteria freeze drying powder suitable for room temperature storage |
| CN107189966B (en) * | 2017-07-13 | 2020-02-18 | 漳州傲农牧业科技有限公司 | Microecological preparation and preparation method thereof |
| CN110477399A (en) * | 2019-03-08 | 2019-11-22 | 韩褆 | A kind of albumen probiotics microbial inoculum and preparation method thereof |
| CN109868246B (en) * | 2019-04-03 | 2021-11-23 | 吉林大学 | Method for freeze-drying protection of rana japonica oil enzymolysis liquid on lactobacillus reuteri |
| CN109797125A (en) * | 2019-04-03 | 2019-05-24 | 吉林大学 | A kind of method of Woodfrog egg protein hydrolysate to lactobacillus reuteri frozen-dried protective |
| CN114052219A (en) * | 2020-08-06 | 2022-02-18 | 重庆康康康生物科技有限公司 | Preparation method and application of leech fermentation liquor |
| CN112457990A (en) * | 2020-12-18 | 2021-03-09 | 中国科学院合肥物质科学研究院 | Freeze-drying protective agent and application thereof |
| CN113100290B (en) * | 2021-05-24 | 2023-03-28 | 宁波大学 | Application of composite fermentation inoculant in preparation of fermented milk |
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| CN101104860A (en) * | 2007-03-22 | 2008-01-16 | 江苏江南生物科技有限公司 | Pupa cordyceps double-enzyme hydrolysis extraction technique |
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| CN1185908A (en) * | 1996-12-27 | 1998-07-01 | 李江 | Functional cordyceps sinensis milk, products made from cordyceps sinensis milk and preparation thereof |
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| CN101015571A (en) * | 2007-03-13 | 2007-08-15 | 陈林生 | Chinese caterpillar fungus polysaccharide buccal tablets and its preparing process |
| CN101104860A (en) * | 2007-03-22 | 2008-01-16 | 江苏江南生物科技有限公司 | Pupa cordyceps double-enzyme hydrolysis extraction technique |
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