CN111134260A

CN111134260A - Dendrobium officinale plant beverage and preparation method thereof

Info

Publication number: CN111134260A
Application number: CN202010103107.4A
Authority: CN
Inventors: 梁洋; 杨槐; 张丽艳
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-02-19
Filing date: 2020-02-19
Publication date: 2020-05-12

Abstract

The invention relates to a dendrobium officinale plant beverage and a preparation method thereof. Specifically, the dendrobium officinale plant beverage is prepared by mixing fermentation liquor obtained by performing enzymolysis and fermentation on dried dendrobium officinale stems and leaves and leaching liquor obtained by leaching the dried dendrobium officinale stems and leaves, wherein the fermentation liquor is calculated by every 1000 mL: the fermentation liquor is prepared from 0.5-1 g (for example, 0.7g) of dried stems and leaves of dendrobium officinale; the leaching liquor is prepared from 1-1.5 g, for example, 1.3g of dried stems and leaves of dendrobium officinale. The dendrobium officinale which is homologous in medicine and food is used as a raw material, so that the medicine material collection is compatible with the industrial production of the beverage, the prepared beverage is high in recovery rate of active substances, excellent in stability, and capable of presenting some excellent technical effects described in the specification, and a popular beverage is provided for the market.

Description

Dendrobium officinale plant beverage and preparation method thereof

Technical Field

The invention belongs to the field of life science products, relates to a dendrobium officinale product, and particularly relates to a beverage prepared from dendrobium officinale. The invention also relates to a method for preparing such a beverage. The dendrobium officinale beverage has incomparable advantages compared with the existing beverage, and can exert the biological effect of the dendrobium officinale by ingestion every day with maximum efficiency as the dendrobium officinale beverage capable of being directly drunk.

Background

Dendrobium officinale (academic name: Dendrobium officinale Kimura et Migo), also known as: HEIHONGCAO, herba Dendrobii, and Dendrobium candidum. Belongs to the field of Microscolecularia, and belongs to the family Orchidaceae, the perennial epiphytic herbaceous plant. The stem is upright and cylindrical, the length is 9-35 cm, the thickness is 2-4 mm, sepals and petals are yellow green and are similar, the long round shape is in the shape of a needle, the length is about 1.8cm, the width is 4-5 mm, and the flowering phase is 3-6 months. Mainly distributed in Anhui, Zhejiang and Fujian provinces of China. The stem of the herb is used as a medicine, which belongs to yin-tonifying herbs in tonifying herbs.

Dendrobium officinale is a perennial herb, widely distributed in Guangxi, Yunnan, Zhejiang, Fujian and other places, and is usually distributed on semi-yin-wet rocks in mountainous regions.

The morphological characteristics of the dendrobium officinale: the stem is upright, cylindrical, 9-35 cm long, 2-4 mm thick, no branch, multiple segments, 1-3-1.7 cm long internode, and 3-5 leaves often grown above the middle; the second row of leaves is made of paper, the long round shape is in a needle shape, the length is 3-4(-7) cm, the width is 9-11(-15) mm, the tip is blunt and is slightly hooked, the lower part of the base part is a sheath for embracing the stem, and the edge and the middle rib are always purplish; the leaf sheath is usually purple-spotted, and when old, the upper edge of the leaf sheath loosens and opens away from the stem, and 1 annular iron blue gap is left between the leaf sheath and the node.

The raceme usually comes out from the upper part of the old stem with fallen leaves and has 2-3 flowers; the inflorescence handle is 5-10 mm long, and the base part is provided with 2-3 short sheaths; the inflorescence shaft is bent in a reverse-folded shape and is 2-4 cm long; the bud sheet is dry, white, egg-shaped, 5-7 mm long, and blunt at its tip; the length of the pedicel and the ovary is 2-2.5 cm; the sepals and the petals are yellow green and similar, the long round shape is in the shape of a needle, the length is about 1.8cm, the width is 4-5 mm, the tip is sharp, and 5 veins are formed; the lateral sepal base is wider and about 1cm wide; the sepal sac is conical, the length is about 5mm, and the tail end is round; the labial lobe is white, the base part is provided with 1 green or yellow callus, the egg shape is in the shape of a needle, the shape is shorter than the sepal, the middle part is reversely folded, the tip is sharp and not cracked or not cracked obviously 3, the two sides below the middle part are provided with mauve stripes, and the edge is wavy; the labial disc is densely covered with hair in the shape of thin papilla, and has 1 purple red plaque above the middle part; the pistil is yellow green and about 3 mm long, and both sides of the tip are respectively provided with 1 purple point; the podophyllum is yellowish green and has purplish red stripes, and hair is dredged; the cap is white, has a long oval triangle shape, is about 2.3 mm long, and has a sharp tip at the top end and 2 cracks. The flowering period is 3-6 months.

In the aspect of growth habit, the dendrobium officinale is suitable for growing in a cool, humid and air-free environment. Usually, the plant grows on the semi-yin and semi-yang rocks in mountainous regions with the elevation of 1600 meters, prefers warm and humid climate and semi-yin and semi-yang environment, and is not cold-resistant.

In terms of geographical distribution, Dendrobium officinale is widely distributed in southwest of Anhui province (Dabieshan), eastern Zhejiang province (37150;, county, Tiantai, Xiju), western Fujian province (Ninghua), northwest of Guangxi province (Tian Anthrig), Sichuan, southeast Yunnan province (stone screen, Wenshan, Ma chestnut slope, West Domain).

In the aspect of propagation methods, there are a tissue rapid propagation method, a plant division cutting propagation method and the like.

A tissue rapid propagation method: hardening seedlings for 14-21 days before cultivating the tissue culture seedlings, and specifically taking the measures that the tissue culture seedlings are moved to a seedling hardening room to gradually adapt to the natural environment in an open changing environment, and the seedlings can be grown in bottles when the leaves of the tissue culture seedlings are dark green.

The bottle cap is opened before the bottle is taken out, and the bottle seedlings are placed in outdoor air for 2-3 days to adapt to natural temperature and humidity. And then washing the seedlings, wherein the seedlings with the culture medium are gently taken out during seedling washing and are placed in a basin for washing, and the polluted seedlings and the bare-rooted seedlings or the seedlings with few roots are separately placed. The tissue culture seedling is firstly washed by tap water, agar is mainly washed off to prevent the agar from mildewing to cause root rot, and then the tissue culture seedling is rinsed by clean water. During seedling washing, classification is preferably carried out synchronously according to the size and the quality of the seedlings so as to facilitate cultivation and management, improve the survival rate of tissue culture seedlings and enable the tissue culture seedlings to grow regularly.

A plant division cutting propagation method: and selecting 1-year or 2-year plants which are tender green, strong, have more germination, developed root systems and have no diseases or insect pests as seed plants during plant division, cutting off dead branches, broken branches, old branches and overlong fibrous roots, cutting the plant clusters, dividing the plant clusters into small clusters, and planting 5-7 stem plants with leaves in each cluster.

In the aspect of cultivation technology, the cultivation time of the dendrobium officinale is two seasons of spring and autumn every year generally, and the spring is superior to the autumn. In Zhejiang, the best time for cultivating the dendrobium officinale is from 4 middle ten days to 6 last ten days of each year, the temperature is 12-25 ℃, the air humidity is high, the transplanting survival rate of test-tube seedlings after the test-tube seedlings are taken out of bottles is high, and the growth time is long; and secondly, from the middle ten days of 9 months to the end ten days of 10 months, the cold-resistant and anti-freezing work is especially required for the transplanting in the period. The culture medium of the dendrobium officinale is a key for high-quality and high-efficiency cultivation, the biological characteristics of the dendrobium officinale require that the culture medium has good water retention and ventilation, and large-scale production requires that the raw materials of the culture medium are easy to obtain and the operation is convenient. In the report, the matrix comprises sphagna, crushed stone, peanut shell, moss, coconut peel, pine bark, wood chip, charcoal, wood block and the like, and the bark and the wood chip or the mixture of the bark, the wood chip, the crushed stone and organic fertilizer are mainly applied in production, wherein the bark is crushed into particles with the particle size of less than 2-3 cm. The dendrobium officinale cultivation is carried out in a greenhouse, the greenhouse is required to be ventilated, shaded and rain-sheltered, an insect-proof net is arranged, and natural factors such as illumination, temperature, humidity, ventilation and the like of a field are considered according to the growth habit of the dendrobium officinale. The suitable growth temperature of the dendrobium officinale is 15-30 ℃, the dendrobium officinale is hot in summer in Zhejiang, and is generally covered by a 80% sunshade net, and a plastic film is opened to facilitate cooling; the winter is cold, and the double-layer plastic film is used for sealing and heat preservation under the sun shading degree of 30-50 percent. The dendrobium officinale needs to keep the matrix moist, the air humidity is preferably kept above 80%, water cannot be accumulated, and the dendrobium officinale is preferably irrigated by adopting spray irrigation or drip irrigation and cannot be irrigated by adopting spray irrigation or drip irrigation. The water pouring amount is different in different regions in different seasons.

CN109757638A (chinese patent application No. 201910156938.5) discloses a preparation method of a dendrobium officinale plant beverage, comprising the following steps: s1: pretreating raw materials; s2: pulping and filtering; s3: extracting effective components: respectively carrying out three times of separation and extraction on the dendrobium stem and leaf filtrate and the dendrobium flower filtrate to obtain a dendrobium stem and leaf extract and a dendrobium flower extract; s4: blending: respectively adding water into the dendrobe stem leaf extracting solution and the dendrobe flower extracting solution to a constant volume to obtain dendrobe stem leaf liquid and dendrobe flower liquid, and then mixing, stirring and homogenizing to obtain a dendrobe beverage; s5: filling and sterilizing: and filling the dendrobium beverage, sterilizing and cooling. The extraction technology and the high-pressure treatment technology of multiple separation and extraction are adopted, so that the full separation of nutrient components is ensured; the PPM is less than or equal to 100, so that the water quality is fully ensured to be healthy; the effects of benefiting stomach and promoting fluid production, protecting liver and benefiting gallbladder, reducing blood sugar and blood fat, and enhancing the body condition are enhanced, and the taste of the beverage is enhanced; economical and efficient, and simultaneously ensures the food safety.

CN109043266A (chinese patent application No. 201811126877.X) discloses a dendrobium officinale beverage and a preparation method thereof, the method comprises the following steps: a. grinding, cleaning fresh herba Dendrobii, cutting into segments, adding pure water, grinding, filtering with a screen to obtain primary pulp and filter residue, adding pure water into the filter residue, grinding for the second time to obtain secondary pulp, and mixing the two pulps to obtain mixed pulp; b. performing enzymolysis, namely adding a complex enzyme into the mixed slurry for enzymolysis to obtain an enzymolysis solution; c. clarifying, adding chitosan into the enzymolysis liquid for clarification treatment to obtain clarified liquid; d. blending, adding citric acid solution and Mel into the clarified solution, and stirring to obtain blended beverage; e. homogenizing, and homogenizing the blended beverage with a high-pressure homogenizer to obtain homogenized beverage; f. and (4) sterilizing, namely sterilizing the homogenized beverage by adopting an ultrahigh-temperature instant sterilization process to obtain the dendrobium officinale beverage. The dendrobium officinale beverage disclosed by the invention is good in flavor and taste, and can improve the immune function of an organism after being drunk for a long time.

CN107136213A (Chinese patent application No. 201710252298.9) discloses a preparation method of dendrobium officinale lactic acid beverage; comprises the following steps of fumigating fresh picked dendrobium officinale, extracting dendrobium polysaccharide, and finally fermenting dendrobium residue to prepare the beverage. The invention separates the extraction process of polysaccharide and the fermentation process of lactic acid bacteria, under the action of ultrasonic waves, cellulase and papain are simultaneously added for combined enzymatic hydrolysis, the cell structure is destroyed, the dendrobium polysaccharide is favorably extracted by water, the extracted dendrobium residue is used for fermentation, a proper amount of auxiliary materials are added, other beneficial components of dendrobium can be extracted in the fermentation process, macromolecular protein is decomposed, and the prepared beverage is rich in nutrition and has the unique fragrance of dendrobium.

CN103300450B (chinese patent application No. 201310238165.8) discloses a method for preparing a primary pulp beverage of dendrobium officinale, comprising: (1) baking fresh branches of dendrobium officinale until the branches are purple, adding water and pulping to obtain primary pulp; (2) boiling the primary slurry after heat preservation treatment, filtering, and taking filter residue; (3) adding water into the filter residue again, pulping, boiling, filtering, taking the filter residue, repeating for several times, adding water into the filter residue, and pulping to obtain secondary pulp; (4) adding cellulase into the secondary slurry for enzymolysis to obtain an enzymolysis solution; (5) boiling the enzymolysis liquid, filtering, and taking filter residue, (6) adding water into the filter residue again, pulping, boiling, filtering, taking the filter residue, and repeating for a plurality of times; (7) and mixing all filtrates, boiling and sterilizing to obtain the dendrobium officinale primary pulp beverage. According to the invention, the dendrobium officinale is baked, and the filter residue is subjected to enzymolysis treatment, so that the extraction rate of functional polysaccharide and flavone is improved, the content of dietary fiber in the beverage is increased, and the prepared beverage is bright and stable in color.

CN107518407A (Chinese patent application No. 201710838617.4) discloses a preparation method of dendrobium officinale health drink, which is characterized by comprising the following steps: (1) selecting and processing raw materials: selecting fresh and healthy dendrobium officinale leaves with a growth period of more than 4-6 months as a raw material, washing the selected dendrobium officinale leaves with running water for 2-3 hours, and draining for later use; (2) crushing: mixing the treated dendrobium officinale leaves with purified water according to the ratio of 1: (1.5-2), placing the mixture into a homogenizing and crushing machine, and homogenizing and crushing for 25-35 minutes to obtain the dendrobium officinale leaf treatment solution; (3) mixing: mixing the dendrobium officinale leaf treatment liquid with fruit juice according to the weight ratio of 1: (0.5-1) to obtain a mixed solution for treating the dendrobium officinale leaves; (4) extraction: adding 0.05-0.1 wt% of neutral cellulase and 0.05-0.1 wt% of neutral pectinase into the mixed solution for treating the dendrobium officinale leaves by weight of the mixed solution for treating the dendrobium officinale leaves, then carrying out ultrasonic treatment, wherein the ultrasonic frequency of the ultrasonic treatment is 20-40 kHz, the stirring speed is 60-120 r/min, the temperature is 25-45 ℃, the treatment time is 65-100 min, then carrying out ultrafiltration by using a 120-160-mesh filter screen, and taking filtrate to obtain a dendrobium officinale leaf extracting solution; (5) concentration: boiling and concentrating the dendrobium officinale leaf extracting solution until the concentration ratio is 1 (0.5-0.6), filtering the dendrobium officinale leaf extracting solution by using a filter screen of 60-80 meshes to obtain a concentrated solution, adding 1-3 wt% of food-grade water-soluble chitosan based on the weight of the concentrated solution, and fully stirring and uniformly dissolving to obtain a concentrated treatment solution; (6) and (3) sterilization and filling: and (3) carrying out ultrahigh-temperature instantaneous sterilization treatment on the concentrated treatment solution, filling and sealing to obtain a finished dendrobium officinale health-care beverage.

The dendrobium officinale stems and leaves are approved to be used as local special food management, so that the dendrobium officinale stems and leaves are beneficial to be used as raw materials of the dendrobium officinale beverage; in addition, the dry products of the stems and leaves of the dendrobium officinale can be used for solving various problems in the processes of harvesting, storing, industrial production and feeding of the dendrobium officinale leaves. The preparation of the dendrobium officinale beverage by using the dried dendrobium officinale stems and leaves is still expected by the technical personnel in the field.

Disclosure of Invention

The invention aims to provide a dendrobium officinale plant beverage, which is expected to have one or more beneficial effects. The invention also aims to provide a preparation method of the dendrobium officinale plant beverage. The dendrobium officinale plant beverage prepared by the invention shows one or more expected technical effects, and the invention is completed based on such findings.

Therefore, the invention provides a dendrobium officinale plant beverage in a first aspect, which is prepared by mixing fermentation liquor obtained by enzymolysis and fermentation of dried dendrobium officinale stems and leaves and leaching liquor obtained by leaching of dried dendrobium officinale stems and leaves.

In the invention, the dry stems and leaves of dendrobium officinale can also be called as the stems and leaves of dendrobium officinale.

In the invention, the dry stem leaves of the dendrobium officinale are dry stems and dry leaves of the dendrobium officinale in a weight ratio of 1-20: 100, or directly drying the harvested stems and leaves of Dendrobium officinale without separating (at this time, the stems and leaves are not specially modified, separated and combined).

In the invention, the dry stem leaves of the dendrobium officinale are dry stems and dry leaves of the dendrobium officinale in a weight ratio of 1-10: 100 in proportion.

In addition, in the invention, when referring to dendrobium officinale, if no special description is provided, the dendrobium officinale refers to stem leaves of dendrobium officinale; when the dendrobium officinale plant beverage is mentioned, if no special description is provided, the dendrobium officinale plant beverage is prepared by using dried stems and leaves of dendrobium officinale.

According to the dendrobium officinale plant beverage provided by the first aspect of the invention, each 1000mL of the fermentation liquid is prepared from 0.5-1 g (for example, 0.7g) of dried stems and leaves of dendrobium officinale.

According to the dendrobium officinale plant beverage provided by the first aspect of the invention, each 1000mL of the leaching solution is prepared from 1-1.5 g, such as 1.3g, of dried stem leaves of dendrobium officinale.

According to the dendrobium officinale plant beverage of the first aspect of the invention, the fermentation liquor is obtained by performing an enzymolysis process on dried stems and leaves of dendrobium officinale to obtain an enzymolysis liquor, and then performing a fermentation process on the obtained enzymolysis liquor.

According to the dendrobium officinale plant beverage of the first aspect of the invention, the enzymolysis liquid is prepared from the following materials in parts by weight:

drying stems and leaves of dendrobium officinale: 0.5 to 1g (e.g., 0.7g),

cellulase: 15-25 u (e.g. 21u),

and (3) pectinase: 45-60 u (e.g. 52.5u),

water: 17.5 mL.

According to the dendrobium officinale plant beverage of the first aspect of the invention, the enzymolysis liquid is prepared according to the following enzymolysis process:

(1) weighing the screened and impurity-removed dry stems and leaves of the dendrobium officinale according to a formula proportion, cleaning the stems and leaves with water, and then crushing the stems and leaves into fragments or broken branches with the size or the length of 0.5-1 cm;

(2) adding the crushed dry stem leaves of dendrobium officinale into water with the prescribed amount, adding cellulase and pectinase with the prescribed amount, uniformly mixing, carrying out enzymolysis for 0.5-2 h (for example, enzymolysis for 1h) at 45-55 ℃, filtering, and inactivating enzyme for 5min at 90 ℃ to obtain the dendrobium officinale enzymolysis liquid.

According to the dendrobium officinale plant beverage of the first aspect of the invention, the fermentation liquor is prepared from the following materials in parts by weight:

and (3) dendrobium officinale enzymolysis liquid: 17.5mL of the solution is added,

glucose: 0.4 to 0.65g (e.g., 0.525g),

fruit and vegetable leavening agent: 1.5 to 2mU (e.g., 1.75 mU).

According to the dendrobium officinale plant beverage of the first aspect of the invention, the fermentation liquor is prepared according to the following fermentation process:

(3) adding glucose in a formula proportion into the dendrobium officinale enzymolysis liquid for dissolving, sterilizing at 95 ℃ for 5-10 min, and then cooling to 37 ℃ to obtain a to-be-fermented dendrobium officinale liquid;

(4) adding a fruit and vegetable leavening agent into the dendrobium officinale liquid to be fermented, stirring at a constant speed at 37 ℃, carrying out anaerobic fermentation for 20-30 hours (for example, 24 hours), immediately cooling to 2-8 ℃ after the fermentation is finished, carrying out coarse filtration by using a 400-mesh screen, carrying out vacuum filtration by using a membrane with the aperture of 0.8 mu m to obtain dendrobium officinale fermentation liquid, and storing at a low temperature of 2-8 ℃ for later use.

According to the dendrobium officinale plant beverage of the first aspect of the invention, the leaching liquor is prepared from the following materials in parts by weight:

drying stems and leaves of dendrobium officinale: 1 to 1.5g, for example, 1.3g,

water: 15-30 mL (e.g., 20-25 mL, e.g., 23mL, e.g., extracted in three portions, e.g., 1 st water with a weight 6-7 times that of the dried stems and leaves, e.g., 2 nd water with a weight 4-6 times that of the dried stems and leaves, e.g., 3 rd water with a weight 4-6 times that of the dried stems and leaves).

According to the dendrobium officinale plant beverage of the first aspect of the invention, the leaching liquor is prepared according to the following leaching process:

(5) weighing the screened and impurity-removed dry stems and leaves of the dendrobium officinale according to a formula proportion, cleaning the dry stems and leaves with water, adding 90-100 ℃ water which is 6-7 times of the weight of the dry stems and leaves of the dendrobium officinale into an extraction tank, extracting for 30-45 min at 90-95 ℃, and filtering by a 300-mesh sieve to obtain a first extract and filter residue of the dry stems and leaves of the dendrobium officinale; adding 90-100 ℃ water which is 4-6 times of the weight of the dried stem leaves of the dendrobium officinale into the obtained filter residues in an extraction tank, extracting for 30-45 min at 90-95 ℃, and filtering by a 300-mesh sieve to obtain a second extract of the dried stem leaves of the dendrobium officinale and filter residues; adding 90-100 ℃ water which is 4-6 times of the weight of the dried stem leaves of the dendrobium officinale into the obtained filter residues in an extraction tank, extracting for 30-45 min at 90-95 ℃, and filtering by a 300-mesh sieve to obtain third extract liquid of the dried stem leaves of the dendrobium officinale and the filter residues; combining the three leaching liquors;

(6) and (3) centrifugally filtering the mixed leaching liquor by using a 800-mesh centrifugal machine, and filtering by using a 0.45-micrometer filter membrane in a plate-and-frame filter to obtain a dendrobium officinale leaching liquor for later use.

According to the first aspect of the invention, the dendrobium officinale plant beverage is prepared from the following materials in parts by weight per 1000 mL:

and (3) dendrobium officinale fermentation liquor: is equivalent to 0.5-1 g (for example 0.7g) of dry stems and leaves of dendrobium officinale,

leaching liquor of dendrobium officinale: is equivalent to the preparation of 1-1.5 g, for example, 1.3g of dried stem leaves of dendrobium officinale,

water: add the appropriate amount to 1000 mL.

According to the first aspect of the invention, the dendrobium officinale plant beverage is prepared according to the following preparation process:

(7) in a high-shear emulsification batching tank, starting shearing, adding the dendrobium officinale fermentation liquor and the dendrobium officinale leaching liquor according to the formula proportion, adding water to a predetermined volume, uniformly stirring, and filtering by a 0.3 mu m membrane to obtain a filtrate;

(8) and (3) carrying out UHT (ultra high temperature sterilization) ultra-high temperature sterilization on the filtrate (for example, UHT ultra-high temperature sterilization conditions include that the temperature of the feed liquid is 137-139 ℃, the sterilization time is 10-15 s), then cooling to 20-25 ℃, and carrying out aseptic filling on the filtrate into beverage bottles to obtain the dendrobium officinale plant beverage.

Further, the invention provides a method for preparing the dendrobium officinale plant beverage in the second aspect, which comprises the step of mixing fermentation liquor obtained by enzymolysis and fermentation of dried dendrobium officinale stems and leaves and leaching liquor obtained by leaching of dried dendrobium officinale stems and leaves.

According to the method of the second aspect of the invention, the fermentation liquid is prepared from 0.5-1 g (for example, 0.7g) of dried stems and leaves of dendrobium officinale, wherein each 1000mL of the dendrobium officinale plant beverage is obtained.

According to the method of the second aspect of the invention, the leaching liquor is prepared from 1-1.5 g, for example, 1.3g, of dried stem leaves of dendrobium officinale, wherein each 1000mL of the dendrobium officinale plant beverage is obtained.

According to the method of the second aspect of the invention, the fermentation liquor is obtained by performing an enzymolysis process on dried stems and leaves of dendrobium officinale to obtain an enzymolysis liquor, and then performing a fermentation process on the obtained enzymolysis liquor.

According to the method of the second aspect of the invention, the enzymolysis liquid is prepared from the following materials in parts by weight:

drying stems and leaves of dendrobium officinale: 0.5 to 1g (e.g., 0.7g),

cellulase: 15-25 u (e.g. 21u),

and (3) pectinase: 45-60 u (e.g. 52.5u),

water: 17.5 mL.

According to the method of the second aspect of the present invention, the enzymatic hydrolysate is prepared according to the following enzymatic hydrolysis process:

(1) weighing the screened and impurity-removed dry stems and leaves of the dendrobium officinale according to a formula proportion, cleaning the stems and leaves with water, and then crushing the stems and leaves into fragments or broken branches with the size or the length of 0.5-1 cm; (needless to say, when the dry stem leaves of the dendrobium officinale of the present invention are crushed, the leaves are crushed into fragments with the size of 0.5-1 cm, and the stems are crushed into branches with the length of 0.5-1 cm)

According to the method of the second aspect of the present invention, the fermentation liquid is prepared from the following materials in parts by weight:

glucose: 0.4 to 0.65g (e.g., 0.525g),

fruit and vegetable leavening agent: 1.5 to 2mU (e.g., 1.75 mU).

According to the method of the second aspect of the present invention, the fermentation broth is prepared according to the following fermentation process:

According to the process of the second aspect of the invention, the leach liquor is prepared from the following materials in parts by weight:

drying stems and leaves of dendrobium officinale: 1 to 1.5g, for example, 1.3g,

According to the process of the second aspect of the invention, the leach liquor is prepared according to the following leach process:

According to the method of the second aspect of the invention, the dendrobium officinale plant beverage is prepared from the following materials in parts by weight per 1000 mL:

water: add the appropriate amount to 1000 mL.

The method according to the second aspect of the present invention comprises the following formulation process steps:

The present inventors have surprisingly found that the addition of lysine and calcium pantothenate in beverages imparts certain or certain superior technical effects to the beverages of the present invention. Therefore, in one embodiment of any aspect of the present invention, lysine hydrochloride and calcium pantothenate are additionally added when mixing the fermentation broth of dendrobium officinale and the leaching solution of dendrobium officinale, so that the concentrations of lysine hydrochloride and calcium pantothenate in the final obtained dendrobium officinale plant beverage are 0.1% and 0.075%, respectively. In the present invention, when% is referred to, unless otherwise specified, the% in the liquid is volume/volume percentage for the liquid and the% in the liquid for the solid, for example, the above calcium pantothenate concentration of 0.075% means 0.075g of calcium pantothenate per 100mL of the liquid.

In the present invention, lysine is used in the form of its hydrochloride unless otherwise specified.

In the above-described steps of the preparation method of the present invention, although the specific steps described therein are distinguished in some detail or in language description from the steps described in the preparation examples of the detailed embodiments below, those skilled in the art can fully summarize the above-described method steps in light of the detailed disclosure throughout the present disclosure.

Any embodiment of any aspect of the invention may be combined with other embodiments, as long as they do not contradict. Furthermore, in any embodiment of any aspect of the invention, any feature may be applicable to that feature in other embodiments, so long as they do not contradict. The invention is further described below.

All documents cited herein are incorporated by reference in their entirety and to the extent such documents do not conform to the meaning of the present invention, the present invention shall control. Further, the various terms and phrases used herein have the ordinary meaning as is known to those skilled in the art, and even though such terms and phrases are intended to be described or explained in greater detail herein, reference is made to the term and phrase as being inconsistent with the known meaning and meaning as is accorded to such meaning throughout this disclosure.

According to records from Shen nong Ben Cao Jing in Qin and Han, the dendrobium officinale Kimura et Migo mainly injures middle energizer, removes arthralgia, purges qi, tonifies five internal organs, makes deficient and consumptive disease, emaciation, strengthens yin and thickens intestines and stomach after long-term use; the famous-region medical classics of Daojiao medical science (Daozha) one thousand years ago sets the dendrobium officinale as the head of the nine-large fairy grass of China; li Shizhen evaluates the dendrobium officinale, namely 'strengthening yin and replenishing essence, thickening intestines and stomach, tonifying interior never enough, calming stomach qi, growing muscles, benefiting intelligence and removing fright, slimming and prolonging life' in 'Bencao gang mu'; the modern dendrobium officinale stem called as 'life saving Mesona chinensis' by folk can clear heat, promote fluid production, diminish inflammation, relieve pain, clear and moisten throat, and has good curative effect on hoarseness. The dendrobium has good effects of resisting fatigue and resisting anoxia. The dendrobium officinale in the pharmacy is various in types and low in price, is usually made of aquatic weeds, only a few large brands in China can buy the real dendrobium officinale, and the dendrobium officinale in the pharmacy is most authentic represented by two old brands, namely the dendrobium officinale in the future and the collemboat. The price of the Tongrentang is the most expensive, the package is beautiful, and the wine is suitable for gift delivery.

Relates to the effect of Dendrobium officinale on treating stomach yin deficiency and body fluid impairment due to fever. It is good at nourishing stomach yin, promoting the production of body fluid to quench thirst, and clearing stomach heat. For body fluid impairment due to heat disease, polydipsia, dry tongue with black coating, it is often combined with Tian Hua fen, Sheng Di Huang and Mai Dong, as in Qing Bao jin from Shi Bing Lun. For epigastric pain, gingival swelling and pain, mouth and tongue sores due to stomach heat and yin deficiency, it is combined with Sheng Di, Mai Dong and Huang Qin, etc.

Relates to the effect of dendrobium officinale on kidney yin deficiency. It can nourish kidney yin and descend deficiency fire, so it is indicated for dim and poor vision, atrophy-flaccidity of tendons and bones, hyperactivity of fire due to yin deficiency, steaming and over-heating of bones due to kidney yin deficiency. For dim and poor vision due to kidney yin deficiency, it is often combined with Gou Qi Zi, Shu Di Huang and Tu Si Zi, for instance, it is used with Shi Hu Huo Lu Wan (original mechanical promoter micro). For atrophy-flaccidity of tendons and bones due to kidney yin deficiency, it is combined with liver and kidney tonics and tendon and bone-strengthening herbs, such as Shu Di Huang, shan Zhu Yu, Du Zhong and niu xi, etc. For bone-steaming and fatigue-heat due to kidney deficiency with effulgent fire, it is combined with kidney-yin-nourishing and deficiency-heat-clearing herbs, such as Sheng Di Huang, Gou Qi Zi, Huang Bai and Hu Huang Lian, etc.

Dendrobium officinale is sweet in flavor and slightly cold in nature. Has the effects of nourishing yin, clearing away heat, reinforcing stomach and promoting fluid production. The herba Dendrobii contains alkaloid such as dendrobine, mucilaginous substance, starch, polysaccharide, mannose, etc. The Dendrobium officinale has effects of relieving fever and pain, promoting gastric secretion, promoting digestion, enhancing metabolism, and resisting aging. Clinically, the dendrobium officinale can be used for treating body fluid damage caused by heat, low heat, polydipsia, red tongue and little coating; deficient stomach yin, thirst, dry throat, vomiting, poor appetite, dull pain in the stomach, and a pale tongue with little coating; kidney yin deficiency, blurred vision.

People have a plurality of eating methods of dendrobium officinale. For example, tea: taking 20-30 g of fresh dendrobium officinale, cleaning, removing clothes, slicing or cutting into sections, cracking, adding a few or a few of Chinese wolfberry fruits, adding a plurality of clear water (according to the drinking amount of the Chinese wolfberry fruits), boiling the water with strong fire, and then boiling the water with slow fire for more than 30 minutes to drink. Or directly drinking with boiling water. Taking out herba Dendrobii for eating after eating. Diet: taking 30-50 g of fresh dendrobium officinale, washing with clear water, slicing or cutting into sections and cracking, and stewing chicken, duck, bone and the like according to personal taste preference. The cooking time is slightly longer than usual. Adding wine: taking 500 g of fresh dendrobium officinale, washing and cleaning with clear water, and soaking 1000 g of medlar, American ginseng and white spirit for 2 months and then taking a proper amount of dendrobium officinale in the morning and at night. Juicing: the tea is cleaned and juiced by a juicer according to the ratio of 10:1, the taste is better by adding honey, and the tea has the effects of sobering up, protecting liver and relieving headache after drinking before drinking and has obvious effect.

The health promotion effect of dendrobium officinale on human bodies is well known. For example:

relates to the function of dendrobium officinale for nourishing yin and body fluid. According to the book of Chinese medicine dictionary, Fengdou is named to nourish lung and stomach qi and promote the flow of qi and liquid, so that kidney water is produced, so that he is good at nourishing yin and promoting the production of body fluid, and can treat various diseases of yin deficiency and body fluid deficiency. The traditional Chinese medicine holds that the yin fluid is the material basis of the life activities of human bodies, has the functions of moistening the body viscera and brain marrow bones and inhibiting the hyperactivity of yang and fire, and can maintain the normal activities of growth, development and reproductive functions. If the yin fluid of the human body is insufficient, symptoms such as listlessness, dull complexion, dry eyes, mental retardation, soreness and weakness of waist and knees, dizziness and hypodynamia, dry mouth and tongue, sore throat, constipation and the like can appear, the maple bucket is the essential medicine for nourishing yin, and the problem of insufficient yin fluid of the human body can be fundamentally solved by taking the maple bucket.

Relates to the physique-strengthening function of dendrobium officinale. Dendrobe has the functions of nourishing yin and blood, and the Qing Dynasty's medical theory says that dendrobe can tonify kidney and accumulate essence, nourish stomach yin and tonify qi. The herba Dendrobii contains abundant polysaccharides. Experiments prove that the dendrobium polysaccharide has the function of enhancing the immunologic function and can enhance the phagocytosis capability of abdominal cavity macrophages on chicken red blood cells.

Relates to the spleen and stomach tonifying effect of dendrobium officinale. The spleen and stomach have the important function of digesting food and taking food essence to nourish the whole body, and are the source of nutrition, so whether the nutrition is sufficient or not depends on the functions of the spleen and stomach. Dendrobe is a stomach-benefiting and fluid-generating medicine, recorded in Shen nong Ben Cao Jing and Ben Cao Xin, and called as an enterogastric medicine by people, and is a common medicine for treating epigastric pain and epigastric pain. Modern experiments prove that the dendrobium has a good inhibition effect on helicobacter pylori which is a common pathogenic bacterium in spleen and stomach diseases, and is beneficial to treating helicobacter pylori positive diseases such as atrophic gastritis, superficial gastritis, duodenal ulcer and the like, and meanwhile, oral dendrobium decoction can promote secretion of gastric juice, enhance emptying capacity of stomach and help digestion.

Relates to the liver protection and gallbladder function of dendrobium officinale. Dendrobium has good cholagogic effect, and doctors of all ages believe that Dendrobium candidum has the effect of nourishing liver yin, is an essential medicine for treating various liver-gallbladder syndromes, and can be used for treating liver-gallbladder diseases such as hepatitis, cholecystitis, cholelithiasis and the like. Clear deficiency heat. Deficiency-heat is manifested by fatigue, weakness, dizziness, flushed face, palpitation, cough, scanty phlegm, insomnia, amnesia, yellowish or reddish urine, red tongue, thready pulse, etc. The compendium of materia medica and supplement of materia medica respectively record the functions of clearing stomach and removing deficiency heat, and the functions of treating hemorrhoids and stomach deficiency heat. Compared with the common heat-clearing medicines, the dendrobium can clear deficiency heat completely, and is characterized by yin deficiency and exuberant heat. Pharmacological experiments show that the dendrobium can obviously reduce the fever peak value and the body temperature response index of the rabbit with the hot condition and shorten the heat development time.

Relates to the function of strengthening tendons and bones of dendrobium officinale. After the person enters middle-aged, the yin body fluid of the human body begins to weaken, the functions of the muscles and the bones are gradually reduced, the dendrobium can nourish yin fluid, and the yin fluid can lubricate joints, so that the effects of strengthening the muscles and the bones, smoothing the joints and enhancing the anti-rheumatism effect are achieved. Modern pharmacological research shows that the dendrobium can improve the stress capability and has good effects of resisting fatigue and resisting anoxia. Promoting circulation, dendrobe can nourish yin, moisten the channels, dilate blood vessels, thereby promoting blood circulation. Experimental results show that the dendrobium can obviously improve the blood stasis symptom, reduce blood cholesterol and triglyceride, improve the level of high-density lipoprotein cholesterol and can treat cardiovascular and cerebrovascular diseases.

Relates to the blood sugar reducing effect of dendrobium officinale. The traditional Chinese medicine considers that the diabetes mainly takes kidney yin deficiency and lung and stomach early heat as basic pathogenesis, takes deficiency of both qi and yin as pathological characteristics, and dendrobium has the functions of nourishing yin, clearing heat and moistening dryness, thus being a special medicine for treating diabetes since ancient times. Clinical research shows that the dendrobium can not only enhance the activity of insulin, but also obviously reduce the blood sugar level and restore the blood sugar to normal.

Relates to the tumor inhibition effect of dendrobium officinale. The dendrobium has killing effect on certain cells of human lung cancer, ovarian cancer and promyelocytic leukemia, and has strong anti-tumor activity. Clinical time shows that the dendrobium is used for adjuvant therapy of malignant tumors, can improve the symptoms of tumor patients, relieve the side effects of radiotherapy and chemotherapy, enhance immunity, improve life quality and prolong life time.

Relates to the eye brightening effect of dendrobium officinale. Traditional Chinese medicine considers that essence and qi of five zang-organs and six fu-organs are injected to eyes through veins, and the physiological pathology of the liver opening into eyes is closely related to the exuberance of yin essence of the liver, and dendrobium has the functions of nourishing yin and eyes, and is taken as a good item for protecting eyes by doctors of different generations. Modern drug efficacy research proves that dendrobium has a delaying effect on preventing and treating cataract which is common ophthalmic disease of the old, can keep thirty-six and eight percent of transparency of crystalline lens, and has an inhibiting or correcting effect on abnormal change of enzyme activity caused by galactose.

Relates to the skin nourishing effect of dendrobium officinale. After the human body enters the middle-aged period, the internal yin fluid is gradually reduced, so that the skin aging is accelerated, and the skin becomes black or wrinkled. The herba Dendrobii contains abundant mucus, and has nourishing and moistening effects on human skin.

Relates to the life prolonging effect of dendrobium officinale. The Shen nong Ben Cao Jing (Shen nong's herbal Jing) lists herba Dendrobii as a commodity medicine with the effects of reducing weight and prolonging life. Modern researches show that the dendrobium contains various trace elements which have close relation to the health and the longevity of human bodies, and the anti-aging effect of the dendrobium on the human bodies is wider and more comprehensive than that of common medicines on the human bodies.

In addition, various classical traditional Chinese medicine theories record the efficacy of the dendrobium officinale in detail. For example, dendrobium officinale is recorded in Bencao gang mu: remove arthralgia and descending qi, nourish internal organs, strengthen yin and nourish essence. After long-term administration, the stomach is thickened, the interior is never filled enough, stomach qi is calmed, muscles are grown, hot miliaria qi is removed, pain and cold arthralgia of feet and knees are weak, intelligence is fixed, convulsion is removed, the body is lightened, and the life is prolonged. Replenishing qi to remove heat, strengthening yang, dispelling wind-evil from skin, keeping bones cold for a long time, tonifying kidney and benefiting strength. It can be used to treat spontaneous sweating, carbuncle, cellulitis and pus discharge. The book records dendrobium officinale from Shen nong Ben Cao Jing: mainly injure middle energizer, remove arthralgia, descend qi, tonify internal organs and consumptive disease, emaciation and yin strengthening. It has effects of improving gastrointestinal function, reducing weight, and prolonging life. The book of Benjing records dendrobium officinale: sweet and mild in taste. Mainly injures middle energizer, removes arthralgia, discharges qi, tonifies five internal organs, makes deficiency, weak, strengthens yin, and thickens intestines and stomach after long-term administration. Compendium (compendium): sweet, bland and slightly salty, it is indicated for spontaneous sweating, abscess, deep-rooted carbuncle and pus-discharging obstruction. The compendium addendum records dendrobium officinale: it can clear stomach, remove deficiency heat, promote the production of body fluid, replace strain and promote appetite and invigorate spleen. Arresting convulsions and treating wind, can suppress salivation and phlegm, and is sweet and fragrant and can direct qi downward.

The eating method of dendrobium officinale is well known.

1. Is eaten fresh. In the past years without antibiotic fever reduction, the fresh dendrobium officinale has special effect on fever of children and has no side effect of antibiotic. For fever caused by various fevers, the fresh dendrobium officinale can be mashed and swallowed with boiled water, or directly chewed, or decocted with boiled water for taking, so that the fever can be relieved, and the adult can also be used for treating deficiency-fire toothache.

2. Making tea. The old goose mountain dendrobium candidum is crushed into powder, 3-5 g/person/day, and the old goose mountain dendrobium candidum is drunk with boiling water for 30 days, so that the special effect is achieved. Or decocting herba Dendrobii with water for more than 30 min for cyclic administration, and eating together with residue after eating on the same day. Has effects in clearing away heat, nourishing yin, and relieving fatigue.

3. Is eaten in diet. The Dendrobium candicum can be added into daily diet, such as stewed chicken and stewed pigeon. The following two examples are referenced: 1) dendrobium candicum porridge: 5g of dendrobium candidum, 50 g of rice and a proper amount of rock sugar. 2) Cordyceps sinensis dendrobium candidum soup: a pair of raw coccykuporus, 5 cordyceps sinensis strips, 5g of dendrobium candidum, 200 g of lean pork and one corner of dried orange peel. Has the curative effect of nourishing yin, promoting the production of body fluid and nourishing liver and kidney, and is suitable for all ages.

4. Fengdou Qingwei Yin. [ raw materials ] 10 g of old goose mountain dendrobium candidum, 12 g of bamboo shavings, 30 g of reed rhizome, 15g of dandelion, 10 g of fructus aurantii, 15g of radix ophiopogonis, 6g of mint, 12 g of white paeony root and 6g of liquorice. [ usage ] 200ml of water is decocted in the morning and evening, and the decoction is taken warmly before meals. [ curative effect ] has effects of refreshing, moistening, regulating qi-flowing and relieving pain. Chronic superficial gastritis and gastric ulcer with preference for heat.

5. Soaking in wine for drinking. Life-prolonging dendrobium candidum wine: 250 g of dendrobium candidum, 60 g of radix rehmanniae recen, 30 g of radix achyranthis bidentatae, 20g of eucommia bark, 20g of salvia miltiorrhiza and 10 liters of white spirit. [ curative effect ] lumbago, skelalgia, tiredness, asthenia, and rheumatic arthralgia. Tonify kidney, strengthen tendons and bones, and relieve itching.

6. Materials of the yin-nourishing and kidney-nourishing soup (1 part by weight): aweto, dendrobium officinale, white paeony root and pulp of dogwood fruit each 15g, mulberry fruit, glossy privet fruit, eclipta alba and prepared rhizome of rehmannia each 25 g. Cooking: decocting in 4 bowls of clear water to 1 bowl. The efficacy is as follows: the cordyceps sinensis can nourish kidney and liver, and the dendrobium officinale, the white paeony root, the mulberry fruit, the cornus officinalis and the like are traditional Chinese medicines for nourishing yin, so that the cordyceps sinensis is most suitable for nourishing liver and kidney of people with yin deficiency.

7. American ginseng dendrobium tea: [ raw materials ] American ginseng 5g, old wild goose mountain officinal dendrobium stem 30 g. The preparation method comprises selecting radix Panacis Quinquefolii, cleaning, sun drying or oven drying, cutting into pieces, and placing into a large container. Putting old Yanshan Dendrobium candicum into an earthen pot, adding 200ml of water, boiling with strong fire, stewing with slow fire for 30 minutes, filtering with clean gauze, removing residues, collecting filtrate, putting into a container containing radix Panacis Quinquefolii decoction pieces, covering and sealing for 15 minutes, and drinking. (or grinding radix Panacis Quinquefolii and herba Dendrobii into fine powder, mixing with boiling water, and drinking instead of tea, 3-5 g each time, 1-2 times daily) the tea is taken as FAN tea, and drinking 2 times in the morning and afternoon frequently. Can be taken instead of tea, and is eaten up in the same day. [ efficacy ] nourishing yin and stomach, promoting the production of body fluid and relieving cough. The formula is suitable for patients with stomach yin deficiency and excess heat type leukemia complicated with stomatitis. [ MEASUREMENT ] American ginseng is cool in nature, sweet and slightly bitter in taste, good at tonifying qi and nourishing yin, clearing fire and promoting the production of body fluid. Modern pharmacological research proves that the American ginseng can improve the disease resistance of organisms and inhibit the growth of cancer cells. The dendrobium has the functions of nourishing stomach yin and promoting the production of body fluid, and clinical research data shows that the dendrobium has the effects of treating thirst, polydipsia, dry throat and tongue, constipation and the like caused by the symptoms of damaging the body fluid after cancer chemotherapy and radiotherapy and yin deficiency and body fluid deficiency. The two medicines are combined into a dietary therapy tea drink, has obvious effects of nourishing yin and stomach, promoting the production of body fluid and relieving cough, has a better auxiliary treatment effect on patients with the stomach yin deficiency heat type leukemia complicated with stomatitis, and is particularly suitable for teenagers, children and elderly leukemia patients.

8. Steaming the dendrobium nobile: raw materials: 1500 g of green snail (conch), 9 g of pork fillet and 6g of dendrobium. Seasoning: a little salt preparation method comprises the following steps: 1) mashing semen Apii Graveolentis, cleaning, scalding with boiling water, and taking out. The soup is filtered for use. 2) Selecting snail meat, cleaning with light saline water, draining, and placing into a stewing cup. The pork fillet is cut into blocks and then is splashed by boiling water to remove blood dirt. 3) Decocting the snail juice and the dendrobium in a small pot for about 20 minutes, removing the medicine residue, and filtering the medicine juice for later use. 4) Pouring the medicinal liquid into a stewing cup, placing the pork fillet on the surface of the snail meat in the cup, stewing for about 1 hour, and adding salt to obtain the product. The characteristics are as follows: the taste is fresh and slightly sweet. The efficacy is as follows: the dendrobium can be stewed with the green snail and the pork to nourish yin, moisten dryness, promote urination, quench thirst and promote diuresis, and can treat emaciation, constipation, dry cough and alcoholic intoxication.

9. Decoction for nourishing Yin and quenching thirst: materials: 6-9 g of dendrobium, 12 g of asparagus, polygonatum, adenophora tetraphylla, rhizoma polygonati, prepared rehmannia root, Chinese yam, poria cocos and 5g of dried orange peel. The use method comprises the following steps: optionally adding appropriate amount of lean meat or chicken, and adding boiling water, and cooking for three hours in eight bowls. The product can be seasoned with salt when eaten. The efficacy is as follows: it is effective in treating diabetes, and liver and stomach yin injury caused by excessive smoking and drinking.

10. Herba dendrobii-semen cassiae granules: materials: dendrobium, American ginseng each 30-50 g, medlar, chrysanthemum, dodder, flatstem milkvetch seed, glossy privet fruit, cassia seed, plantain seed each 60 g, and salvia miltiorrhiza 60-80 g. The use method comprises the following steps: the above medicines are ground into fine powder. Twice a day, 3-5 g each time. It is administered after mixing with boiling water. The effect is better when the medicine is continuously taken for more than three months. The efficacy is as follows: can be used for treating hypertension, glaucoma, and cataract due to deficiency of liver-yin and kidney-yin.

11. Tea beverage: the polygonatum, the coastal glehnia root, the dendrobium, the dwarf lilyturf tuber each 15g and five dark plums are decocted with water to obtain juice, and a proper amount of rock candy is added to replace tea for drinking, so that the tea can be used for treating mouth dryness and excrement dryness caused by heat disease yin impairment or excessive sweating in summer. 12. 15g of coastal glehnia root, 10 g of dried rehmannia root, dwarf lilyturf tuber and dendrobium respectively and a proper amount of rock sugar are decocted with water for oral administration, which is suitable for the symptoms of body fluid deficiency, dry throat and mouth, inappetence, constipation, difficult urination and the like after hyperpyrexia.

The usage and dosage of Dendrobium candicum are as follows: 1. adopting a low-temperature cell wall breaking grinding method: grinding into fine powder of 200 meshes or more (without pulverizing with high-speed heating type common pulverizer, and breaking medicinal molecules due to high temperature generated during crushing). 3g of dendrobium candidum fine powder is taken every day and taken with warm boiled water twice a day in the morning and evening (if qi is required to be supplemented, a small amount of American ginseng or ginseng powder can be added for taking together). The recommended method has the best effect and is suitable for long-term administration. 2. Ancient method wide soup is decocted for a long time: taking 5-6 g (two days) of raw materials of the dendrobium candidum, adding 1.5L of water, and stewing and boiling the mixture in an earthen pot. The decoction is taken after twenty-four hours of first boiling, eight hours of second boiling, eight hours of third boiling, and eight hours of fourth boiling, and the decoction and the dendrobium officinale residue are taken together. The good dendrobium candidum is fragrant and tender in quality, less in residue, high in viscosity and clear and not greasy in taste after being boiled, and can be directly drunk without adding any seasoning. Suitable for long-term administration [ if qi is required to be supplemented, a little radix Panacis Quinquefolii or Ginseng radix can also be added ]. 3. 3-5 g of raw dendrobium candidum is taken and stewed with chicken and duck for 100 minutes by slow fire, and the raw dendrobium candidum is eaten together with residues.

As is well known, the whole body of dendrobium officinale is precious, the roots, stems, leaves and flowers of the dendrobium officinale have basically the same chemical composition and physiological function, and the roots and the flowers are very difficult to collect, so that the dendrobium officinale is difficult to effectively utilize in industry; the stem used as a conventional medicine is convenient to collect, and is also very convenient to dry, store and dispose, but in the process of collecting and processing the stem part, the by-product leaves are usually generated, and the dendrobium officinale loaded in pharmacopeia is not leaves, but in the process of collecting the stem, the leaves are usually dried to different degrees, and the collection of fresh leaves of the dendrobium officinale as in the literature is obviously difficult to meet the industrial production. Therefore, if the dendrobium officinale can be effectively utilized, especially in a form of drying stems and leaves, the expansion of the industrial utilization rate of the dendrobium officinale can be facilitated. According to the invention, the dendrobium officinale dry stems and leaves which are easy to obtain and dispose are prepared into the beverage, so that the dendrobium officinale can be more effectively and comprehensively used, and a popular beverage is provided for the market, so that the various effects of the dendrobium officinale can be utilized to improve the physical constitution of a body.

Detailed Description

The present invention will be further described by the following examples, however, the scope of the present invention is not limited to the following examples. It will be understood by those skilled in the art that various changes and modifications may be made to the invention without departing from the spirit and scope of the invention. The present invention has been described generally and/or specifically with respect to materials used in testing and testing methods. Although many materials and methods of operation are known in the art for the purpose of carrying out the invention, the invention is nevertheless described herein in as detail as possible. The following examples further illustrate the invention without limiting it.

The following preparation steps are given for the purpose of illustration and are based on the comparative nature of the respective examples and the person skilled in the art is fully enabled to generalize from the prior knowledge the process of the invention for preparing the products of the invention.

In the following examples of preparing the dendrobium officinale beverage of the present invention, the processing amount of each beverage is not less than 2000L, but is listed in convenient units such as mL (mL), g (g), u (unit), mU (milliunit) when listing the prescription, and further double dosing in proportion to these units when actually dosing to reach the prescribed amount of each beverage preparation, as not otherwise specified.

Example 1: preparation of Dendrobium officinale plant beverage

In this embodiment, the dry stem leaves of dendrobium officinale used are dry stems of dendrobium officinale and dry leaves of dendrobium officinale in a weight ratio of 5: 100, in a mixture.

1. Dendrobium officinale enzymolysis liquid

The formula of the enzymolysis liquid comprises:

drying stems and leaves of dendrobium officinale: 0.7g of a mineral oil in the form of,

cellulase: 21u of the number of the magnetic poles are 21u,

and (3) pectinase: 52.5u of the total weight of the steel,

water: 17.5 mL.

The preparation method of the enzymolysis liquid comprises the following steps:

(2) adding the crushed dry stem leaves of dendrobium officinale into water with the prescribed amount, adding cellulase and pectinase with the prescribed amount, uniformly mixing, carrying out enzymolysis for 1h at 50-55 ℃, filtering, and inactivating enzyme for 5min at 90 ℃ to obtain the dendrobium officinale enzymolysis liquid.

The cellulase is purchased from Shandong Kete enzyme preparation company, and the activity is 20 ten thousand u/g; pectinase was purchased from Shandong Kete enzyme preparations with a viability of 30 wu/g, and this is the same in the specific examples herein, unless the source is noted.

2. Dendrobium officinale fermentation liquor

The formula of the fermentation liquid is as follows:

glucose: 0.525g of a non-woven fabric,

fruit and vegetable leavening agent: 1.75 mU.

The preparation method of the fermentation liquor comprises the following steps:

(4) adding a fruit and vegetable leavening agent into the dendrobium officinale liquid to be fermented, stirring at a constant speed at 37 ℃, carrying out anaerobic fermentation for 24 hours, immediately cooling to 2-8 ℃ after the fermentation is ended, carrying out coarse filtration by using a 400-mesh screen, carrying out vacuum filtration by using a membrane with the pore diameter of 0.8 mu m to obtain dendrobium officinale fermentation liquid, and storing at a low temperature of 2-8 ℃ for later use.

The fruit and vegetable leavening agent is lactic acid bacteria powder PPS-401 purchased from Beijing Ke Tuhengtong biotechnology, Inc., and the source is not indicated in the specific examples.

3. Dendrobium officinale extract

The formula of the leaching liquor is as follows:

drying stems and leaves of dendrobium officinale: 1.3g of a mixture of (A) and (B),

water: extracting in three times, namely water 6.7 times the weight of the dried stems and leaves at the 1 st time, water 5 times the weight of the dried stems and leaves at the 2 nd time, and water 5 times the weight of the dried stems and leaves at the 3 rd time.

The preparation method of the leaching liquor comprises the following steps:

(5) weighing the screened and impurity-removed dry stems and leaves of the dendrobium officinale according to a formula proportion, cleaning the dry stems and leaves with water, adding water with a specified weight into an extraction tank at 90-100 ℃, extracting for 30-45 min at 90-95 ℃, and filtering by a 300-mesh sieve to obtain a first extract and filter residues of the dry stems and leaves of the dendrobium officinale; leaching the filter residue twice by the same method, and combining leaching liquor obtained by leaching for three times;

4. Dendrobium officinale plant beverage

The formula of the dendrobium officinale plant beverage comprises the following components:

and (3) dendrobium officinale fermentation liquor: obtained as above, is equivalent to being prepared from 0.7g of dried stem leaves of dendrobium officinale,

leaching liquor of dendrobium officinale: obtained as above, is equivalent to being prepared from 1.3g of dried stem leaves of dendrobium officinale,

water: add the appropriate amount to 1000 mL.

The preparation method of the dendrobium officinale plant beverage comprises the following steps:

In the specific example herein, the sterilized beverage is filled into 350ml beverage bottles and sealed in the final step, as not specifically described.

Example 2: preparation of Dendrobium officinale plant beverage

1. Dendrobium officinale enzymolysis liquid

The formula of the enzymolysis liquid comprises:

drying stems and leaves of dendrobium officinale: 0.5g of a mineral oil in the form of a mineral oil,

cellulase: 15u of the number of the holes are arranged in the groove,

and (3) pectinase: 45u of the total weight of the steel,

water: 17.5 mL.

The preparation method of the enzymolysis liquid comprises the following steps:

(2) adding the crushed dry stem leaves of dendrobium officinale into water with the prescribed amount, adding cellulase and pectinase with the prescribed amount, uniformly mixing, carrying out enzymolysis at 50-55 ℃ for 0.5h, filtering, and inactivating enzyme at 90 ℃ for 5min to obtain the dendrobium officinale enzymolysis liquid.

2. Dendrobium officinale fermentation liquor

The formula of the fermentation liquid is as follows:

glucose: 0.4g of a mineral oil in the form of a mineral oil,

fruit and vegetable leavening agent: 2 mU.

(4) adding a fruit and vegetable leavening agent into the dendrobium officinale liquid to be fermented, stirring at a constant speed at 37 ℃, carrying out anaerobic fermentation for 20 hours, immediately cooling to 2-8 ℃ after the fermentation is ended, carrying out coarse filtration by using a 400-mesh screen, carrying out vacuum filtration by using a membrane with the pore diameter of 0.8 mu m to obtain dendrobium officinale fermentation liquid, and storing at a low temperature of 2-8 ℃ for later use.

3. Dendrobium officinale extract

The formula of the leaching liquor is as follows:

drying stems and leaves of dendrobium officinale: 1 to 1.5g, for example, 1.3g,

water: extracting in three times, namely water 6 times the weight of the dried stems and leaves at the 1 st time, water 6 times the weight of the dried stems and leaves at the 2 nd time, and water 6 times the weight of the dried stems and leaves at the 3 rd time.

The preparation method of the leaching liquor comprises the following steps:

4. Dendrobium officinale plant beverage

and (3) dendrobium officinale fermentation liquor: obtained as above, is equivalent to being prepared from 0.5g of dried stem leaves of dendrobium officinale,

leaching liquor of dendrobium officinale: obtained as above, is equivalent to being prepared from 1.5g of dried stem leaves of dendrobium officinale,

water: add the appropriate amount to 1000 mL.

Example 3: preparation of plant beverage containing dendrobium officinale and dendrobium officinale

1. Enzymolysis liquid of dendrobium officinale

The formula of the enzymolysis liquid comprises:

drying stems and leaves of dendrobium officinale: 1g of the total weight of the mixture is obtained,

cellulase: 25u of the number of the magnetic poles are arranged in the magnetic pole,

and (3) pectinase: 60u of the total weight of the steel,

water: 17.5 mL.

The preparation method of the enzymolysis liquid comprises the following steps:

(2) adding the crushed dry stem leaves of dendrobium officinale into water with the prescribed amount, adding cellulase and pectinase with the prescribed amount, uniformly mixing, carrying out enzymolysis for 2 hours at 45-50 ℃, filtering, and inactivating enzyme for 5 minutes at 90 ℃ to obtain the enzymatic hydrolysate of dendrobium officinale.

2. Fermentation liquor of dendrobium officinale

The formula of the fermentation liquid is as follows:

glucose: 0.65g of a coarse powder is added,

fruit and vegetable leavening agent: 1.5 mU.

(4) adding a fruit and vegetable leavening agent into the dendrobium officinale liquid to be fermented, stirring at a constant speed at 37 ℃, carrying out anaerobic fermentation for 30h, immediately cooling to 2-8 ℃ after the fermentation is ended, carrying out coarse filtration by using a 400-mesh screen, carrying out vacuum filtration by using a membrane with the pore diameter of 0.8 mu m to obtain dendrobium officinale fermentation liquid, and storing at a low temperature of 2-8 ℃ for later use.

3. Leaching liquor of dendrobium officinale

The formula of the leaching liquor is as follows:

drying stems and leaves of dendrobium officinale: 1 to 1.5g, for example, 1.3g,

water: extracting in three times, namely 1 time of water with the weight 7 times that of the dried stems and leaves, 2 times of water with the weight 4 times that of the dried stems and leaves, and 3 times of water with the weight 4 times that of the dried stems and leaves.

The preparation method of the leaching liquor comprises the following steps:

(6) and (3) centrifugally filtering the mixed leaching liquor by using a 800-mesh centrifugal machine, and filtering by using a 0.45-micrometer filter membrane in a plate-and-frame filter to obtain leaching liquor of the dendrobium officinale for later use.

4. Dendrobium officinale plant beverage

and (3) dendrobium officinale fermentation liquor: obtained as above, is equivalent to being prepared from 1g of dried stem leaves of dendrobium officinale,

leaching liquor of dendrobium officinale: obtained as above, is equivalent to being prepared from 1g of dried stem leaves of dendrobium officinale,

water: add the appropriate amount to 1000 mL.

Example 4: preparation of Dendrobium officinale plant beverage

Referring to the formulation and preparation of example 1, except that the fruit and vegetable leavening agent used was the microbial probiotic lactic acid bacterium Yo-cul295, both cellulase and pectinase were obtained from eastern Henghua Corp.

Example 5: preparation of Dendrobium officinale plant beverage

Referring to the formula and the preparation method of example 1, the difference is that the dry stem leaves of dendrobium officinale used in the formula are the dry stems of dendrobium officinale and the dry leaves of dendrobium officinale in a weight ratio of 1: 100. 10: 100. 20: 100, or directly drying the stem and leaf of Dendrobium officinale, i.e. the mixture of Dendrobium officinale and herba DendrobiiThe stem/leaf ratio was (a) 1: 100. (b) 10: 100. (c) 20: 100. (d) comparing naturally four cases, thereby obtaining four casesDendrobium officinale plant beverage, which can be respectively referred to as examples 5a, example 5b, example 5c, example 5d。

The beverage bottles used in the above embodiments 1 to 5 are all aluminum pop cans.

Test example 1: quality index investigation

The results obtained for the beverages obtained in the examples herein are given below with reference to the general quality index of the beverages:

note: in each step of preparing the beverage according to each example of the present invention, no pH adjustment was performed, and the water used in each example was double distilled water. The method for observing the precipitate comprises the following steps: the beverage is shaken in an original packaging bottle and poured into a glass beaker, and whether precipitates exist or not is continuously observed for 0-10 minutes.

According to the above results, it can be seen that the beverage prepared by the present invention has excellent quality properties.

Test example 2: stability survey

The aluminum pop can in the embodiments 1 to 5 is placed in a position with a temperature of 40 ℃ in a built-in package for 6 months, and after 6 months, the detection is carried out according to the method/index of the test example 1, and the results are as follows:

index (I)

Evaluation criteria

Example 1

Example 2

Example 3

Example 4

Example 5

Appearance of the product

Colorless to pale yellow uniform transparent liquid

Meets the standard

Smell of nourishing

The product has good taste and no odor

Meets the standard

Soluble solid

≥0.1g/L

0.22g/L

0.21g/L

0.25g/L

0.23g/L

0.22～0.27g/L

pH

4.5～5.0

4.80

4.75

4.68

4.93

4.67～4.91

Precipitate

Should be free of precipitate

No precipitate

Hygiene indicators

Conforms to GB7101

Meets the standard

As can be seen from the above table results, each beverage sample had excellent stability.

Test example 3: examination of extraction Rate of mannose

According to a method for measuring mannose by high performance liquid chromatography under the item of dendrobium officinale variety carried on page 282 of the first section of the 'chinese pharmacopoeia 2015 edition', the mannose content in the raw medicinal materials of dendrobium officinale in examples 1-5 is measured (the measurement results are all in the range of 18.6% -22.9%, for example, the mannose content in the raw medicinal materials used in example 1 is 19.6%), the mannose content in the beverage finally obtained in each example is measured, and the percentage of mannose entering the beverage from the medicinal materials is calculated by combining the total volume of the beverage and the total weight of the raw medicinal materials, wherein the percentage is the mannose recovery rate. As a result, the recovery rates of mannose in the beverages of examples 1 to 4 were as follows: 92.4%, 90.6%, 91.2%, 89.7%, and the recovery rate of mannose in the beverage of example 5 was 91.4-93, 1%, which indicates that the recovery rate of mannose as an important active substance in the process of preparing the beverage by the method of the present invention was very high.

By adopting the method, the mannose recovery rate in the methods for preparing the dendrobium officinale beverage in the prior art is investigated. A beverage is prepared by the method described in the specification [0010] to [0013] of CN107136213A, namely, the method described in the embodiment 1, the feeding amount of the fresh dendrobium is measured, the content of mannose in the fresh dendrobium is measured, the content of the mannose in the obtained beverage and the total amount of the obtained beverage are measured, and the recovery rate of the mannose is calculated to be 51.1 percent. A beverage was prepared by referring to the descriptions [0044] to [0049] of CN103300450B, namely the method described in example 2, and the amount of fresh dendrobe in the herb and the content of mannose in the herb were measured, and the content of mannose in the obtained beverage and the total amount of the obtained beverage were measured to calculate the recovery rate of mannose, which was 63.6%. Referring to the specification [0013] of CN107518407A, namely, the method described in the first embodiment, the method for preparing the beverage comprises the steps of measuring the feeding amount of the fresh dendrobium leaves serving as the medicinal material and the content of mannose in the fresh dendrobium leaves, measuring the content of the mannose in the obtained beverage and the total amount of the obtained beverage, and calculating the recovery rate of the mannose, wherein the result is 58.7%. From the results, the recovery rate of the beverage prepared by the method for preparing the beverage for the important active substance mannose in the dendrobium officinale is obviously higher than that of the method in the prior art.

Test example 4: beverage Performance inspection

In the case of preparing the beverages in examples 1 to 5, when the final beverage was filled into 350ml beverage bottles, 50L of the final beverage was taken and packaged in 350ml Plastic (PET) beverage bottles.

Supplementary example a: referring to the formula and the preparation method of examples 1 to 5, respectively, except that in the step (7) of preparing the dendrobium officinale plant beverage at the final stage, when the dendrobium officinale fermentation liquid and the dendrobium officinale leaching liquor are mixed, lysine hydrochloride and calcium pantothenate are additionally added so that the concentrations of the lysine hydrochloride and the calcium pantothenate in the finally obtained beverage are 0.1% and 0.075%, respectively, the eight prepared beverages are marked as supplement A1, supplement A2, supplement A3, supplement A4 and supplement A5 (namely, four of supplement A5a, supplement A5b, supplement A5c and supplement A5 d), and the eight beverages of supplement A1-supplement A5 are respectively packaged by adopting 350ml Plastic (PET) beverage bottles and aluminum pop-top cans.

Supplementary example B: referring to the formulation and the preparation method of example 1, except that in the step (7) of preparing the dendrobium officinale plant beverage at the final stage, lysine hydrochloride (the concentration of lysine hydrochloride in the finally obtained beverage is 0.1%) or calcium pantothenate (the concentration of calcium pantothenate in the finally obtained beverage is 0.075%) is additionally added when the dendrobium officinale fermentation broth and the dendrobium officinale leaching liquor are mixed, so as to prepare two beverages, namely a supplement B1 and a supplement B2, and the two beverages, namely the supplement B1 and the supplement B2, are respectively packaged by adopting 350ml Plastic (PET) beverage bottles and aluminum pop cans.

The beverages of supplement A1-supplement A5 and supplement B1-supplement B2 only add supplement substances in the final stage, and do not influence the recovery rate of the active substance mannose. The above beverages, i.e., beverages supplemented with A1 to A5 and B1 to B2, were evaluated for their quality index by the method of test example 1, and they all had substantially the same index as in the results of example 1, had soluble solids increased by 0.004 to 0.015g/L (which substantially coincides with the theoretical amount of lysine and calcium pantothenate added), had pH values substantially unchanged and in the range of 4.5 to 5.0, and had no precipitate in the initial state (including the beverages of examples 1 to 5 dispensed using plastic bottles).

Next, the stability of the different packaged beverages was examined in accordance with the method of test example 2. After standing at 40 ℃ for 6 months, the beverages of examples 1-5, the beverages of supplement A1-supplement A5 and the beverages of supplement B1-supplement B2, which were dispensed from pop cans or plastic bottles, were identical or substantially unchanged from the initial state of each beverage in appearance, flavor, soluble solids, pH and hygienic index. However, with respect to sediment, different formulations of beverages with different packaging materials showed completely different results, in particular after 6 months at 40 ℃: (1) the beverages of examples 1 to 5, the beverages of supplement A1 to supplement A5 and the beverages of supplement B1 to supplement B2, which were dispensed by pop cans, all had no precipitate (the beverages were left to stand for 6 months without shaking, and the remaining bottles were carefully sucked out to make about 20ml of the bottom, and the soluble solids of the beverages were measured to be all in the range of 0.21 to 0.28g/L and the difference from the soluble solids at 40 ℃ to 0 months was in the range of + -0.025 g/L, for example, the difference between the soluble solids at 40 ℃ to 6 months of supplement B2 and the soluble solids at 40 ℃ to 0 months was 0.016 g/L); (2) the beverages of Bu A1-Bu A5 dispensed by plastic bottles have no precipitate (the beverages are not shaken after being placed for 6 months, the rest bottle bottom is sucked out carefully to make about 20ml, and the soluble solids of the beverages are all in the range of 0.20-0.28 g/L and the difference with the soluble solids at 40-0 months is in the range of +/-0.020 g/L, for example, the difference between the soluble solids at 40-6 months of Bu A1 and the soluble solids at 40-0 months is 0.011 g/L); (3) the beverages of examples 1 to 5 and the beverages of Bu B1 to Bu B2 dispensed by plastic bottles all had significant precipitates (the beverages were left to stand for 6 months without shaking, and the remaining bottles were carefully sucked out to make about 20ml of the bottom of the bottle, and the soluble solids of the beverages were measured to be in the range of 1.46 to 1.62g/L and the difference from the soluble solids at 40 ℃ to 0 months was in the range of 1.21 to 1.42g/L, for example, the difference from the soluble solids at 40 ℃ to 6 months of Bu B1 to the soluble solids at 40 ℃ to 0 months was 1.33 g/L). It can be seen that: for the beverage added with lysine and calcium pantothenate simultaneously, the beverage has excellent stability and particularly does not generate precipitates no matter the beverage is subpackaged by a pop can or a plastic bottle; the beverage added with neither lysine nor calcium pantothenate or only one of lysine and calcium pantothenate does not generate precipitates when split charging is carried out by using pop cans, but the precipitates can generate precipitates when split charging is carried out by using plastic bottles; this indicates that the beverage of the present invention may have insufficient stability in terms of formation of precipitates in plastic bottles, and that the beverage to which neither lysine nor calcium pantothenate is added is excellent in stability in terms of formation of precipitates when dispensed from plastic bottles, but insufficient stability may cause precipitates when dispensed from plastic bottles, whereas the beverage to which both lysine and calcium pantothenate are added is applicable to both packaging materials and does not cause precipitates. This stability effect provides the possibility to provide the market with a more popular beverage. Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention, and not for limiting the same; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. A herba Dendrobii plant beverage is prepared by mixing fermentation broth obtained by enzymolysis and fermentation of dried stem and leaf of herba Dendrobii with leaching liquor obtained by leaching dried stem and leaf of herba Dendrobii.

2. The dendrobium officinale plant beverage according to claim 1, which is characterized in that:

the dry stem leaves of the dendrobium officinale are dry stems and dry leaves of the dendrobium officinale, and the weight ratio of the dry stems to the dry leaves is 1-20: 100, or a stem and leaf mixture obtained by directly drying stems and leaves harvested from dendrobium officinale without separating;

every 1000mL of the fermentation liquor is prepared from 0.5-1 g (for example, 0.7g) of dried stems and leaves of dendrobium officinale; and/or

The leaching liquor is prepared from 1-1.5 g, for example, 1.3g of dried stem leaves of dendrobium officinale in per 1000 mL.

3. The dendrobium officinale plant beverage according to claim 1, wherein the fermentation broth is obtained by subjecting dried stems and leaves of dendrobium officinale to an enzymolysis process to obtain an enzymolysis solution, and then subjecting the obtained enzymolysis solution to a fermentation process.

4. The dendrobium officinale plant beverage according to claim 1, wherein the enzymolysis solution is prepared from the following materials in parts by weight:

drying stems and leaves of dendrobium officinale: 0.5 to 1g (e.g., 0.7g),

cellulase: 15-25 u (e.g. 21u),

and (3) pectinase: 45-60 u (e.g. 52.5u),

water: 17.5 mL; and/or

The enzymolysis solution is prepared according to the following enzymolysis process:

5. The dendrobium officinale plant beverage according to claim 1, wherein the fermentation liquid is prepared from the following materials in parts by weight:

glucose: 0.4 to 0.65g (e.g., 0.525g),

fruit and vegetable leavening agent: 1.5 to 2mU (e.g., 1.75 mU); and/or

The fermentation liquor is prepared according to the following fermentation process:

6. The dendrobium officinale plant beverage according to claim 1, wherein the leaching liquor is prepared from the following materials in parts by weight: drying stems and leaves of dendrobium officinale: 1 to 1.5g, for example, 1.3g,

water: 15-30 mL (e.g., 20-25 mL, e.g., 23mL, e.g., extracted in three portions, e.g., 1 st water with a weight 6-7 times that of the dried stems and leaves, e.g., 2 nd water with a weight 4-6 times that of the dried stems and leaves, e.g., 3 rd water with a weight 4-6 times that of the dried stems and leaves); and/or

The leaching liquor is prepared according to the following leaching process:

7. The dendrobium officinale plant beverage according to claim 1, which is prepared from the following materials in parts by weight per 1000 mL:

water: adding a proper amount of the mixture to 1000 mL; and/or

It is prepared according to the following preparation process:

8. The Dendrobium officinale plant beverage according to claims 1-7, wherein lysine hydrochloride and calcium pantothenate are additionally added when the fermentation liquid of Dendrobium officinale and the leaching solution of Dendrobium officinale are mixed.

9. The method for preparing the dendrobium officinale plant beverage as defined in any one of claims 1 to 8, which comprises the step of mixing a fermentation liquid obtained by enzymolysis and fermentation of dried dendrobium officinale stems and leaves with a leaching liquid obtained by leaching of dried dendrobium officinale stems and leaves.

10. The method according to claim 9, comprising the steps of:

(2) adding the crushed dry stem leaves of dendrobium officinale into water with the prescribed amount, adding cellulase and pectinase with the prescribed amount, uniformly mixing, carrying out enzymolysis for 0.5-2 h (for example, enzymolysis for 1h) at 45-55 ℃, filtering, and inactivating enzyme for 5min at 90 ℃ to obtain an enzymatic hydrolysate of dendrobium officinale;

(4) adding a fruit and vegetable leavening agent into the dendrobium officinale liquid to be fermented, stirring at a constant speed at 37 ℃, carrying out anaerobic fermentation for 20-30 hours (for example, 24 hours), immediately cooling to 2-8 ℃ after the fermentation is finished, carrying out coarse filtration by using a 400-mesh screen, carrying out vacuum filtration by using a membrane with the aperture of 0.8 mu m to obtain dendrobium officinale fermentation liquid, and storing at a low temperature of 2-8 ℃ for later use;

(6) centrifuging and filtering the mixed leaching solution with a 800-mesh centrifuge, and filtering with a 0.45 μm filter membrane in a plate-and-frame filter to obtain herba Dendrobii leaching solution for use;