CN106072578A - There is the enhancing enzyme composition of immune function of human body, effervescent tablet and preparation method - Google Patents
There is the enhancing enzyme composition of immune function of human body, effervescent tablet and preparation method Download PDFInfo
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- 230000001953 sensory effect Effects 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
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- 230000003860 sleep quality Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 229940023144 sodium glycolate Drugs 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
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- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
- JEJAMASKDTUEBZ-UHFFFAOYSA-N tris(1,1,3-tribromo-2,2-dimethylpropyl) phosphate Chemical compound BrCC(C)(C)C(Br)(Br)OP(=O)(OC(Br)(Br)C(C)(C)CBr)OC(Br)(Br)C(C)(C)CBr JEJAMASKDTUEBZ-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/40—Effervescence-generating compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention relates to have the enzyme composition strengthening immune function of human body, this enzyme composition includes following component: probiotic bacteria 1 15 weight portion, enzyme composition stock solution 10 120 weight portion and Chinese medicine extract 10 80 weight portion；Described enzyme composition is to mix with enzyme composition stock solution after the sterilized technique of Chinese medicine extract, and inoculates beneficial flora and carry out sealed fermenting and form；Chinese medicine extract is extracted by following bulk drugs and forms: Radix Panacis Quinquefolii 2 10 weight portion, Semen Ginkgo 2 10 weight portion, Ganoderma 18 weight portion, Radix Et Caulis Acanthopanacis Senticosi 2 10 weight portion, the Cortex Eucommiae 28 weight portion, Caulis Spatholobi 18 weight portion, Herba Inulae cappae 0.5 6 weight portion, Rhizoma Curculiginis 0.5 6 weight portion, Rhizoma Cyperi 0.2 3 weight portion and Fructus Lycii 0.5 5 weight portion.Probiotic bacteria and functional oligose are used in combination by this enzyme composition, and add Chinese medicine ingredients, reach to improve effect of body immunity.
Description
Technical field
The invention belongs to field of health care food, particularly to a kind of have strengthen immune function of human body enzyme composition,
Effervescent tablet and preparation method.
Background technology
Ferment is in recent years to rise in Japan, a kind of functional food of TaiWan, China, and it is with fermented by mixed bacterium such as yeast
The plant fermented liquid of more than 50 kinds fruit and vegetable gained.Ferment is rich in the functional cometabolism from more than 50 kinds fruit and vegetable materials
Product, such as polyphenol and oligosaccharide.The at present research to fruit-vegerable ferment focuses mostly on adjusting and improving at raw material, strain and fermentation technology
Aspect, also occurs in that the health food that some add the ferment of probiotic bacterias and prepared by ferment simultaneously.Probiotics fermention fruit and vegerable, can
Activate the effective efficiency composition in fruit and vegerable, active effect material of dissolution more elite.As number of patent application is
201510865242.1 patent application document disclosed in the manufacture method of a kind of Cordyceps militaris (L.) Link. Fructus actinidiae chinensis ferment improving immunity；
And for example number of patent application be 201610108666.8 patent application document disclosed in a kind of fruit and vegerable active ferment liquid, by will be many
Planting sarcocarp and vegetable sealed fermenting prepares, enzyme liquid is the multi-nutrition maintaining human body in a basic balance, improves body immunity.But
It is that this ferment composition is simple, and functional the best, ferment product is fruit and vegerable fermentation liquid, and fluid product is unfavorable for carrying transport.
Inadequate to the concern of mouthfeel, quality and the dosage form of ferment finished product for present stage, applicant feels the need to into one
The Exploitative potential of step exploitation ferment, to obtain the ferment based food that mouthfeel is adjustable controlled and is convenient for carrying.
Summary of the invention
Present invention seek to address that technical problem present in prior art.To this end, it is an object of the present invention to propose
A kind of have the enzyme composition strengthening immune function of human body, and probiotic bacteria and functional oligose are combined and make by this enzyme composition
With, and add Chinese medicine ingredients, make said composition while having good intestinal microbial population regulatory function, it is possible to promote little point
Son sugar and polysaccharide are to the conversion of oligosaccharide；Give full play to effect of the Chinese medicine extract enhancing immunity of enzyme composition, be suitable for
Crowd in hypoimmunity.
Technical scheme is as follows:
A kind of have the enzyme composition strengthening immune function of human body, and this enzyme composition includes following component: probiotic bacteria
1-15 weight portion, enzyme composition stock solution 10-120 weight portion and Chinese medicine extract 10-80 weight portion；Described enzyme composition is
Mix with enzyme composition stock solution after the sterilized technique of Chinese medicine extract, and inoculate beneficial flora and carry out sealed fermenting and form；
Described enzyme composition stock solution is by being the hermetic container of 30-40 DEG C in temperature after fruit and vegerable impregnation liquid inoculation beer yeast
Middle fermentation 2-5d and obtain；Wherein beer yeast inoculum concentration is the 7-10% of fruit and vegerable stock solution weight；
Described Chinese medicine extract is extracted by following bulk drugs and forms:
Radix Panacis Quinquefolii 2-10 weight portion, Semen Ginkgo 2-10 weight portion, Ganoderma 1-8 weight portion, Radix Et Caulis Acanthopanacis Senticosi 2-10 weight portion, Cortex Eucommiae 2-
8 weight portions, Caulis Spatholobi 1-8 weight portion, Herba Inulae cappae 0.5-6 weight portion, Rhizoma Curculiginis 0.5-6 weight portion, Rhizoma Cyperi 0.2-3 weight portion and
Fructus Lycii 0.5-5 weight portion.
The Chinese medicine extract of the present invention is share by more than Ten Herbs compatibility all medicines, respectively executes its merit, the most mutually association's medicine gesture,
Play the merit of strengthening vital QI to eliminate pathogenic factors altogether, reach to improve effect of body immunity.Probiotic bacteria coordinates fermentation with enzyme composition stock solution, can
Activate the effective efficiency composition in fruit and vegerable, active effect material of dissolution more elite.Make with Chinese medicine extract reasonable combination simultaneously
With so that it is and between probiotics fermention thing, there is mutual promoting action, it is possible to the multiplication regulatory function to intestinal microbial population, it is right to improve
The absorption of Effective Component of Chinese Medicine, realizes improving effect of immunity further.Additionally, the enzyme composition stock solution added is by fruit and vegerable
Impregnation liquid inoculation beer yeast carries out primary fermentation technique, improves enzyme composition of the present invention from fermentability, is ferment group
In compound, each effective ingredient can be discharged more fully.
Further reasonable disposition fruit and vegerable impregnation liquid, described fruit and vegerable impregnation liquid is mixed by fruit and vegetable materials, remelt sugar and pure water
Making through ozone sterilization after standing 24h, the ratio of weight and number of described fruit and vegetable materials, remelt sugar and water is 3-4:1:6, and described fruit and vegerable are former
Material is Fructus Pruni pseudocerasi and Fructus actinidiae chinensis.
Further, described probiotic bacteria is probiotic bacteria powder, following at least two powder be uniformly mixed: bifid bar
Bacterium 1.5-3 weight portion, lactobacillus rhamnosus 2-2.5 weight portion, lactobacillus casei 1.2-2 weight portion, bacillus acidophilus 1.4-2.5
Weight portion and Lactobacillus plantarum 2-3 weight portion, the viable bacteria content of described probiotic bacteria powder is 1 × 108-5×108cfu/g。
The functional oligose added in enzyme composition of the present invention is growth of probiotics substrate, has and promotes probiotic bacteria
The physiological function of propagation, the further multiplication regulatory function to intestinal microbial population, thus effect based on intestinal microbial population plays more preferably
Enhancing immunity；In concrete scheme, described enzyme composition also includes chitosan 10-50 weight portion, inulin 5-30 weight
Part and vitamin B group's 12-35 weight portion.
In further preferred version, this enzyme composition includes following component: probiotic bacteria 12.5 weight portion, ferment group
Compound stock solution 86 weight portion, Chinese medicine extract 40.7 weight portion, chitosan 26 weight portion, inulin 18 weight portion and vitamin B
Group's 22.5 weight portions；
Wherein, described Chinese medicine extract is extracted by following crude drug and forms:
Radix Panacis Quinquefolii 5.8 weight portion, Semen Ginkgo 4.5 weight portion, Ganoderma 5 weight portion, Radix Et Caulis Acanthopanacis Senticosi 4.5 weight portion, the Cortex Eucommiae 3.2 weight
Part, Caulis Spatholobi 4 weight portion, Herba Inulae cappae 3.5 weight portion, Rhizoma Curculiginis 3.5 weight portion, Rhizoma Cyperi 1.5 weight portion and Fructus Lycii 2 weight portion.
For improving property easy to use and the storage time limit of enzyme composition, present invention also offers one and there is enhancing human body
The effervescent tablet of immunologic function, effervescent tablet is made up of above-mentioned enzyme composition and adjuvant, described compositions and the parts by weight of adjuvant
Ratio is 1:0.5-1:3.
Wherein, described adjuvant includes sodium starch glycol 35-80 weight portion, sodium bicarbonate 60-132 weight portion, citric acid
35-80 weight portion, maltose alcohol 10-40 weight portion, cherry syrup 30-60 weight portion, sodium palmitate 16-52 weight portion, tristearin
Acid zinc 10-30 weight portion.
In adjuvant, by being used in conjunction with of sodium starch glycol and maltose alcohol, it is beneficial to improve stability and the sheet of tablet
The dispersed homogeneous degree of active component in agent, improves the bioavailability of effective ingredient in tablet, assists compositions or strengthens combination
Thing effect, improves compositions curative effect；Cherry syrup has flavored action, effectively covers the bad smell of Chinese medicine；Sodium palmitate is with hard
The cohesive force being used in conjunction with improving between compositions further of fat acid zinc, improves tablet molding.
Design is learnt after deliberation, in effervescent tablet of the present invention during the following scheme of each proportioning raw materials, has raising immunity optimal
Curative effect, and effervescent tablet stability, molding and bioavailability are the most optimal.Concrete, effervescent tablet is by following enzyme composition and auxiliary
Expect processed being prepared from:
Enzyme composition: 187.7 weight portions；
Adjuvant: sodium starch glycol 64 weight portion, sodium bicarbonate 118 weight portion, citric acid 45 weight portion, maltose alcohol 28
Weight portion, cherry syrup 51 weight portion, sodium palmitate 34 weight portion and zinc stearate 20 weight portion.
It is a further object to provide the preparation method of a kind of above-mentioned enzyme composition, described method includes following
Step:
(1) preparation of Chinese medicine extract:
(1.1) first take the Semen Ginkgo of described weight portion, Ganoderma and Radix Panacis Quinquefolii to pulverize, cross 60 mesh sieves, load in extraction kettle,
It is passed through CO with 15-25L/ hour flow velocity2, add 15% ethanol and make entrainer, be 30MPa condition at temperature holding 45 DEG C, pressure
Lower extraction 4h, carries out separating for the first time, collects crude extract, and crude extract goes successively to separating still and carries out secondary separation, in pressure
Power is 12MPa, temperature be 45 DEG C under the conditions of extract 3.5h, extract is put into pillar seperator separate, obtain upper strata grease and
The watery thing of lower floor；Taking off the watery thing of layer, add ethyl acetate and extract three times, separatory funnel is layered, and collects water layer, extracts three times
Water layer merge, add activated carbon roguing, obtained solution 1；
(1.2) Herba Inulae cappae of recipe quantity, Radix Et Caulis Acanthopanacis Senticosi, the Cortex Eucommiae, Caulis Spatholobi, Rhizoma Curculiginis, Rhizoma Cyperi and Fructus Lycii, boiling two are weighed
Secondary, each 1h；Amount of water is add raw material gross weight 10 times for the first time；Amount of water is add raw material gross weight 8 times for the second time；Close
And decoction liquor, filter, obtain solution 2；
(1.3) solution 2 mix homogeneously that the solution 1 step (1.1) prepared and step (1.2) prepare, obtains Chinese medicine extraction
Thing；
(2) preparation of enzyme composition stock solution: added to by remelt sugar in fruit and vegetable materials, is simultaneously introduced pure water mixing all
Even, after standing extraction 24h, carry out ozone sterilization, filter, obtain planting dip；It is 8% that fruit and vegerable impregnation liquid is inoculated into inoculum concentration
Beer yeast, in the hermetic container that temperature is 30-40 DEG C ferment 3d, obtain enzyme composition stock solution；
(3) Chinese medicine extract and enzyme composition stock solution are mixed addition probiotic bacteria, chitosan, inulin and vitamin B group,
Ferment in the hermetic container that temperature is 30-40 DEG C 5-7d, filters, collects filtrate, and filtrate carries out low temperature spray drying, obtains powder
The enzyme composition of shape.
Semen Ginkgo, Ganoderma and the Radix Panacis Quinquefolii extracted by step (1), the extraction ratio of Effective Component of Chinese Medicine is high.By above-mentioned
Enzyme composition prepared by step, improves directly interpolation probiotic bacteria and prepares the traditional handicraft of enzyme composition, and ferment combination
Thing self has nutritional labeling splendid in curative effect medically and dietetic therapy；It also offers a kind of brand-new enzyme preparation, logical
Cross this enzyme composition and be prepared as various preparation, it is simple to carry and eat.
In preparation prepared by enzyme composition of the present invention, being preferably prepared into effervescent tablet, being directly dissolved in water can use,
And rate of dissolution is very fast, need not be by the most available sufficiently release of mixing plant effective ingredient in the effective process time.Tool
The preparation method of body is as follows:
A. prepare the enzyme composition of powdery, weigh formula ratio and cross 60 mesh sieves, stand-by；
B. the sodium starch glycol and the maltose alcohol that weigh formula ratio put into baking oven, dry 3h, cooling under the conditions of 60 DEG C
Rear mistake 60 mesh sieve, stand-by；
C. weigh the sodium bicarbonate of formula ratio, dissolve with the ethanol solution of 60-80%, add the step a process of 1/2 weight
Enzyme composition and the sodium starch glycol that processes of step b of 1/2 weight and maltose alcohol, make soft material, wet granulation,
Cold drying, granulate, obtain bicarbonate soda granule；
D. the citric acid weighing formula ratio is mixed homogeneously with cherry syrup, dissolves with the ethanol solution of 60-80%, adds 1/
Enzyme composition that step a of 2 weight processes and the sodium starch glycol that step b of 1/2 weight processes and maltose alcohol, system
Become soft material, wet granulation, cold drying, granulate, obtain granulates；
Granulates mixing prepared by the bicarbonate soda granule e. step c prepared and step d, adds sodium palmitate with hard
Fat acid zinc uniformly mixes, tabletting, prepares effervescent tablet.
The Chinese medicinal components added in the enzyme composition of the present invention has its physiologically active and health-care effect, is prebiotic simultaneously
Bacterium supplements the nutrients in fermentation, has higher practical value, and the Chinese medicine ingredients of the present invention can be effectively improved body immunity.
Implant multiple probiotic bacteria symbiotic fermentation to form, containing abundant organized enzyme, and contain the useful vitamin group of all multipair human bodies, ore deposit
Material, trace element.Between probiotic bacteria, functional oligose, vitamin B group and each component of Chinese medicine, compatibility is reasonable, and curative effect is bright
Really, there is the effect of Synergistic；Absorption and the metabolism of health can be adjusted, effectively solve liver dispelling toxicity, detoxicating and absorption of human body
And the problem of metabolism.
This ferment effervescent tablet is convenient for carrying, and wherein retains and has the Chinese medicine extract effective ingredient improving immunity, also protects
Stay ferment polyphenol, and coordinated oligosaccharide to use the contents of saccharide reduced in ferment, improve products taste, and can be as required
Regulation；Effervescent tablet preparation formulation further improves the stability of ferment polyphenol.This enzyme composition and effervescent tablet appropriate immune thereof
People's use that power is the best, takes as daily beverage, improves internal metabolism from the angle effected a permanent cure, it is achieved the purpose of health care.
Additionally, the technical process that enzyme composition solid preparation is prepared in enzyme composition directly interpolation is simple, it is easy to accomplish
Large-scale production, and safe preparation process, have good application prospect.
The source of the raw material of Chinese medicine composition of the present invention:
Radix Panacis Quinquefolii: this product is the dry root of Araliaceae Radix Panacis Quinquefolii Panax quinquefolium L., has QI invigorating
Yin nourishing, clearing away heat and promoting production of body fluid effect；For deficiency of vital energy yin deficiency, interior-heat, cough with asthma expectorant blood, deficiency-heat is tired of tired, quenches one's thirst, dryness of the mouth and throat.
Semen Ginkgo: this product is the drying and ripening kind of Ginkgoaceae plant Ginkgo biloba (maidenhair tree, Gong Sunshu) Ginkgo biloba L.
Son；Having gas of astringing the lung, relieving asthma and cough, leukorrhagia stopping is turbid, urine reducing effect；For controlling asthma, phlegmatic cough, leucorrhea, nebulousurine, seminal emission, gonorrhea is little
Just frequency.
Ganoderma: this product is On Polyporaceae Ganoderma lucidum (Leyss. Ex Fr.) Karst. Ganodermalucidum (Leyss.exFr.) Karst. or Ganoderma
Dry sporophore.
Radix Et Caulis Acanthopanacis Senticosi: this product is Araliaceae Radix Et Caulis Acanthopanacis Senticosi Acanthopanax senticosus (Rupr.et Maxim.)
Dry, the root and rhizome of Harms or stem.
The Cortex Eucommiae: this product is the dry bark of Eucommiaceae plant Cortex Eucommiae Eucommia ulmoides Oliv..
Caulis Spatholobi: this product is leguminous plant spatholobus suberectus (Caulis Sargentodoxae, Radix seu Caulis Verntilaginis Leiocarpae, SANYE Caulis Spatholobi, nine layers of wind)
The dry rattan of Spatholobus suberectus Dunn.
Herba Inulae cappae: for Compositae Inulaplants Herba Inulae cappae Inula cappa DC., with root or all herbal medicine.
Rhizoma Curculiginis: this product is the dry rhizome of amrallid Rhizoma Curculiginis Curculigo orchioides Gaertn..
Rhizoma Cyperi: this product is the dry rhizome of sedge dried tuber CyperusrotundusL..There are promoting QI circulation for relieving depression, adjust
Meridian and relieving pain effect；For stagnation of QI due to depression of the liver, breast, the side of body, abdominal distention, dyspepsia, breast gastral cavity painful abdominal mass is vexed, and colic of cold type is suffered from abdominal pain, distending pain of the breast, the moon
Through uncomfortable, amenorrhea dysmenorrhea.
Fructus Lycii: this product is the dry mature fruit of plant of Solanaceae lycium barbarum Lycium barbarum L..
Detailed description of the invention
Embodiment 1 enzyme composition
The prescription of the enzyme composition of the present embodiment is:
Probiotic bacteria 40g, plant enzyme stock solution 100g, Chinese medicine extract 100g；
Wherein, probiotic bacteria is made up of following probiotic bacteria powder: bacillus bifidus 15g, lactobacillus rhamnosus 25g；
Plant enzyme stock solution is prepared from by following raw material: Fructus Pruni pseudocerasi 20g, Fructus actinidiae chinensis 10g, remelt sugar 10g, pure water 60g, beer
Brewer yeast 7g；
Chinese medicine extract is prepared from by following raw material: Radix Panacis Quinquefolii 40g, Semen Ginkgo 40g, Ganoderma 20g, Radix Et Caulis Acanthopanacis Senticosi 40g, the Cortex Eucommiae
40g, Caulis Spatholobi 20g, Herba Inulae cappae 10g, Rhizoma Curculiginis 10g, Rhizoma Cyperi 4g, Fructus Lycii 10g.
Wherein, enzyme composition stock solution preparation method is: select maturation, without rot Fructus Pruni pseudocerasi and Fructus actinidiae chinensis, Fructus Pruni pseudocerasi epidermis clean,
Stripping and slicing after Fructus actinidiae chinensis peeling, addition remelt sugar and pure water, mix homogeneously, after standing 24h, ozone sterilization, prepare plant extraction liquid；
Inoculation beer yeast is to plant extraction liquid, and ferment in the hermetic container of temperature 30-40 DEG C 2d, obtains enzyme composition stock solution.
Embodiment 2 enzyme composition
The prescription of the enzyme composition of the present embodiment is:
Probiotic bacteria 150g, plant enzyme stock solution 1200g, Chinese medicine extract 800g；
Wherein, probiotic bacteria is made up of following probiotic bacteria powder: lactobacillus rhamnosus 50g, lactobacillus casei 100g；
Plant enzyme stock solution is prepared from by following raw material: Fructus Pruni pseudocerasi 300g, Fructus actinidiae chinensis 133g, remelt sugar 108g, pure water
650g, beer yeast 119g；
Chinese medicine extract is prepared from by following raw material: Radix Panacis Quinquefolii 200g, Semen Ginkgo 200g, Ganoderma 160g, Radix Et Caulis Acanthopanacis Senticosi 200g,
Cortex Eucommiae 160g, Caulis Spatholobi 160g, Herba Inulae cappae 120g, Rhizoma Curculiginis 120g, Rhizoma Cyperi 60g, Fructus Lycii 100g.
Wherein, enzyme composition stock solution preparation method is: select maturation, without rot Fructus Pruni pseudocerasi and Fructus actinidiae chinensis, Fructus Pruni pseudocerasi epidermis clean,
Stripping and slicing after Fructus actinidiae chinensis peeling, addition remelt sugar and pure water, mix homogeneously, after standing 24h, ozone sterilization, prepare plant extraction liquid；
Inoculation beer yeast is to plant extraction liquid, and ferment in the hermetic container of temperature 30-40 DEG C 5d, obtains enzyme composition stock solution.
Embodiment 3 enzyme composition
The prescription of the enzyme composition of the present embodiment is:
Probiotic bacteria 125g, enzyme composition stock solution 860g, Chinese medicine extract 407g, chitosan 260g, inulin 180g and
Vitamin B group 225g；
Wherein, probiotic bacteria is made up of following probiotic powder: bifid bar 50g, lactobacillus casei 50g, Lactobacillus plantarum 25g；
Enzyme composition stock solution is prepared from by following raw material: Fructus Pruni pseudocerasi 225g, Fructus actinidiae chinensis 225g, remelt sugar 150g, pure water
900g, beer yeast 115g；
Described Chinese medicine extract is extracted by following crude drug and forms: Radix Panacis Quinquefolii 116g, Semen Ginkgo 90g, Ganoderma 100g, Radix Et Caulis Acanthopanacis Senticosi
90g, Cortex Eucommiae 64g, Caulis Spatholobi 80g, Herba Inulae cappae 70g, Rhizoma Curculiginis 70g, Rhizoma Cyperi 30g and Fructus Lycii 40g；
Preparation method:
(1) preparation of Chinese medicine extract:
(1.1) first take the Semen Ginkgo of described weight portion, Ganoderma and Radix Panacis Quinquefolii to pulverize, cross 60 mesh sieves, load extraction kettle
In, it is passed through CO with 15-25L/ hour flow velocity2, add 15% ethanol and make entrainer, be 30MPa bar at temperature holding 45 DEG C, pressure
Extracting 4h under part, carry out separating for the first time, collect crude extract, crude extract goes successively to separating still and carries out secondary separation,
Pressure is 12MPa, temperature extracts 3.5h under the conditions of being 45 DEG C, extract is put into pillar seperator and separates, obtain upper strata grease
Thing watery with lower floor；Taking off the watery thing of layer, add ethyl acetate and extract three times, separatory funnel is layered, and collects water layer, extracts three times
The water layer taken merges, and adds activated carbon roguing, obtained solution 1；
(1.2) Herba Inulae cappae of recipe quantity, Radix Et Caulis Acanthopanacis Senticosi, the Cortex Eucommiae, Caulis Spatholobi, Rhizoma Curculiginis, Rhizoma Cyperi and Fructus Lycii, boiling two are weighed
Secondary, each 1h；Amount of water is add raw material gross weight 10 times for the first time；Amount of water is add raw material gross weight 8 times for the second time；Close
And decoction liquor, filter, obtain solution 2；
(1.3) solution 2 mix homogeneously that the solution 1 step (1.1) prepared and step (1.2) prepare, obtains Chinese medicine extraction
Thing, takes 407g standby；
(2) preparation of enzyme composition stock solution: select maturation, without rot Fructus Pruni pseudocerasi and Fructus actinidiae chinensis, Fructus Pruni pseudocerasi epidermis clean, Mi
Stripping and slicing after the peeling of monkey Fructus Persicae, addition remelt sugar and pure water, mix homogeneously, after standing 24h, ozone sterilization, prepare plant extraction liquid；Connect
Kind beer yeast is to plant extraction liquid, and ferment in the hermetic container of temperature 30-40 DEG C 4d, obtains enzyme composition stock solution, takes
860g is standby；
(3) Chinese medicine extract and enzyme composition stock solution are mixed, add probiotic bacteria, chitosan, inulin and vitamin B
Group, ferment in the hermetic container that temperature is 30-40 DEG C 5-7d, filters, collects filtrate, and filtrate carries out low temperature spray drying,
Enzyme composition to powdery.
Embodiment 4 enzyme composition
The prescription of the enzyme composition of the present embodiment is:
Probiotic bacteria 20g, enzyme composition stock solution 450g, Chinese medicine extract 200g, chitosan 500g, inulin 50g and dimension
Raw element B group 120g；
Wherein, probiotic bacteria is made up of following probiotic powder: bacillus bifidus 8g, lactobacillus rhamnosus 7g, bacillus acidophilus 5g；
Enzyme composition stock solution is prepared from by following raw material: Fructus Pruni pseudocerasi 80g, Fructus actinidiae chinensis 55g, remelt sugar 45g, pure water
270g, beer yeast 40g；
Described Chinese medicine extract is extracted by following crude drug and forms: Radix Panacis Quinquefolii 70g, Semen Ginkgo 60g, Ganoderma 40g, Radix Et Caulis Acanthopanacis Senticosi
50g, Cortex Eucommiae 50g, Caulis Spatholobi 40g, Herba Inulae cappae 20g, Rhizoma Curculiginis 20g, Rhizoma Cyperi 10g and Fructus Lycii 20g.
Preparation method: prepare according to the method for embodiment 3.
Embodiment 5 effervescent tablet
Use the enzyme composition 1877g of embodiment 3；
Adjuvant: sodium starch glycol 640g, sodium bicarbonate 1180g, citric acid 450g, maltose alcohol 280g, cherry syrup
510g, sodium palmitate 340g and zinc stearate 200g.
Preparation method comprises the steps:
A. prepare the enzyme composition of powdery, weigh formula ratio and cross 60 mesh sieves, stand-by；
B. the sodium starch glycol and the maltose alcohol that weigh formula ratio put into baking oven, dry 3h, cooling under the conditions of 60 DEG C
Rear mistake 60 mesh sieve, stand-by；
C. weigh the sodium bicarbonate of formula ratio, dissolve with the ethanol solution of 60-80%, add the step a process of 1/2 weight
Enzyme composition and 1/2 weight step b process sodium starch glycol and maltose alcohol, make soft material, wet method system
Grain, cold drying, granulate, obtain bicarbonate soda granule；
D. the citric acid weighing formula ratio is mixed homogeneously with cherry syrup, dissolves with the ethanol solution of 60-80%, adds 1/
Enzyme composition that step a of 2 weight processes and the sodium starch glycol that step b of 1/2 weight processes and maltose alcohol, system
Become soft material, wet granulation, cold drying, granulate, obtain granulates；
Granulates mixing prepared by the bicarbonate soda granule e. step c prepared and step d, adds sodium palmitate with hard
Fat acid zinc uniformly mixes, tabletting, prepares effervescent tablet.
Embodiment 6 effervescent tablet
Use the enzyme composition 1850g of embodiment 4；
Adjuvant: sodium starch glycol 350g, sodium bicarbonate 600g, citric acid 350g, maltose alcohol 100g, cherry syrup
300g, sodium palmitate 160g and zinc stearate 100g.
Preparation method: prepare according to preparation method described in embodiment 5.
Embodiment 7 effervescent tablet
Use the enzyme composition 2160g of embodiment 3；
Adjuvant: sodium starch glycol 800g, sodium bicarbonate 1320g, citric acid 800g, maltose alcohol 400g, cherry syrup
600g, sodium palmitate 520g and zinc stearate 300g.
Preparation method: prepare according to preparation method described in embodiment 5.
Comparative examples 1
Use the enzyme composition of embodiment 3: 1877g；
Adjuvant: sodium bicarbonate 1180g, citric acid 450g, cherry syrup 510g, sodium palmitate 340g and zinc stearate
200g。
Preparation method: in addition to being not added with sodium starch glycol and maltose alcohol, all according to preparation method system described in embodiment 5
Standby.
Comparative examples 2
Use the enzyme composition of embodiment 3: 1877g；
Adjuvant: sodium starch glycol 640g, sodium bicarbonate 1180g, citric acid 450g, maltose alcohol 280g, sodium palmitate
340g and zinc stearate 200g.
Preparation method: in addition to being not added with cherry syrup, all prepares according to preparation method described in embodiment 5.
Comparative examples 3
Use the enzyme composition of embodiment 3: 1877g；
Adjuvant: identical with adjuvant described in embodiment 5 and consumption thereof.
Preparation method comprises the steps:
A. prepare the enzyme composition of powdery, weigh formula ratio and cross 60 mesh sieves, stand-by；
B. the sodium starch glycol and the maltose alcohol that weigh formula ratio put into baking oven, dry 3h, cooling under the conditions of 60 DEG C
Rear mistake 60 mesh sieve, stand-by；
C. weigh the sodium bicarbonate of formula ratio, add enzyme composition and formula ratio starch that formula ratio step a processes
Sodium glycolate and maltose alcohol, mix homogeneously, it is dried, granulate, obtains bicarbonate soda granule；
D. the citric acid weighing formula ratio is mixed homogeneously with cherry syrup, is dried, granulate, obtains granulates；
Granulates mixing prepared by the bicarbonate soda granule f. step c prepared and step d, adds colloidality silicon dioxide
Uniformly mix with zinc stearate, tabletting, prepare effervescent tablet.
Test example 1 mouthfeel is tested
The sample 10g of Example 3, embodiment 5 and matched group embodiment 2 so that it is be dissolved in the water of 200ml, stirring, system
Obtain need testing solution；Often group allows 10 professional test persons taste, and compares and takes rear mouthfeel；Standards of grading divide 4 grades: mouthfeel
Difference, with bitter taste, 1 point；Mouthfeel is poor, with slight bitter taste 2 points；Good in taste, without bitter taste, 3 points；Mouthfeel
Good, taste is sweet, without bitter taste 4 points；Test result is shown in Table 1；
The sensory test result of table 1 enzyme composition and effervescent tablet
From table 1 it follows that the embodiment of the present invention 5 formula preferably improves the mouthfeel of effervescent tablet, reduce the bitterness of medicine
Taste, the comparison with comparative examples 2 understands, and cherry syrup can effectively correct former disagreeable taste；Can obtain from the result of embodiment 1
Going out, though the enzyme composition mouthfeel of the present invention is good not as embodiment 5, but mouthfeel still accepts for people.
Test example 2 stability test
1. accelerated test
Example 5 and the effervescent tablet of comparative examples 1, select 3 batches and test, and the sample number into spectrum of embodiment 5 is sample
1, sample 2 and sample 3；The sample number into spectrum of comparative examples 1 is sample 4, sample 5, sample 6；In temperature40℃±2℃Under, phase
To humidity it is75% ± 5%Under conditions of place 6 months, testing 1 month period, 2 months, 3 months, 6 the end of month take respectively
Sample once, by the regulation in Chinese Pharmacopoeia, detects the character of effervescent tablet, the content (labelled amount %) of ginsenoside, and result is sent out
Existing, the effervescent tablet indices of embodiment 5 has no significant change, and the effervescent tablet of comparative examples 1 tablet surface when 3 months becomes
Coarse, fragment occurs, when 6 months, effervescent tablet has sliver.Understanding, the interpolation of sodium starch glycol and maltose alcohol is favorably improved
The stability of effervescent tablet.
2. long term test
The effervescent tablet of Example 5, selects 3 batches and tests, respectively numbered sample 1, sample 2 and sample 3, in temperature25℃±2℃Under, relative humidity is60% ± 10%Under conditions of place 24 months, test 0 month period, 3 months, 6
Month, 9 months, 12 months, 18 months, 24 the end of month separately sampled once, by the regulation in Chinese Pharmacopoeia, detection embodiment 5
The character of effervescent tablet, the content (labelled amount %) of ginsenoside, it was found that indices has no significant change；To according to the facts
The effervescent tablet tablet surface when 3 months executing example 1 is roughening, fragment occurs, and when 6 months, effervescent tablet has sliver.Understanding, starch is sweet
The interpolation of alkyd sodium and maltose alcohol is favorably improved the stability of effervescent tablet.
Test example 3 slaking test
With reference to the regulation of Effervescent tablet disintegration test under 2010 editions annex of Chinese Pharmacopoeia, the embodiment of the present invention 5 effervescent tablet is entered
Row disintegrative detects, and detection method is as follows:
Example 5 effervescent tablet 12 is for for examination group, and comparative examples 3 effervescent tablet 12 is matched group, and every weight is equal
For 3g, add normal-temperature water 200ml, stand 30min.Result of the test is；
For examination group: the complete disintegrate in 3.5min in normal-temperature water of all effervescent tableies, solution clarification (show to have dissolved
Entirely), without muddy, taste fragrance.
Matched group: effervescent tablet is complete disintegrate in 5-10min, slightly cloudy, taste fragrance.
More than test shows, effervescent tablet preparation method of the present invention is mixed by substep, the preparation of mixing after substep soft material processed
Effervescent tablet disintegration prepared by method is better, dissolution can discharge the property of medicine rapidly, has taking convenience, dissolution rapid release spy
Property, reach intended galenic pharmacy effect.
Test example 4 pharmacodynamics test
1 material
1.1 given the test agent are the enzyme composition that embodiment 3 prepares
1.2 laboratory animals and environment: SPF level KM female mice, body weight 18-22g；Laboratory animal room temperature: 22～25 DEG C,
Relative humidity: 55～70%.
2 methods and result
Basic, normal, high three dosage: 2.5g/kg bw, 5g/kg bw, 10g/kg bw are set；Negative control is set simultaneously
Group.Test group gives after using the deployed concentration of distilled water, and negative control group directly gives distilled water, gavage mice 30 days.
The mouse spleen lymphocyte transformation experiment of 2.1 ConA inductions
Giving after each group of continuous gavage 30d of test mice by prescribed dose, cervical dislocation puts to death mice, aseptic takes spleen, makes
Single cell suspension (cell concentration is 1 × 107/mL).Mouse boosting cell suspension is separately added in 24 well culture plates, each place
Reason uses two multiple holes, every hole 1mL, and a hole adds 50 μ LConA, and the 50 μ L distilled waters that another hole adds, as comparison, then will
Culture plate is placed in 5%CO2, the CO2 gas incubator of 37 DEG C is cultivated 48 hours.Cultivation terminates front 4h, adds MTT (5mg/
ML) 50 μ L/ hole, continues to cultivate, and cultivates after terminating, and every hole adds 1mL DMSO, piping and druming mixing, makes purple crystal be completely dissolved,
Then it is dispensed in 96 well culture plates, the multiple hole of every hole subpackage 3, at 570nm wavelength, measures OD value by microplate reader.With adding hole
Optical density value deduct and be not added with the optical density value in hole and represent the multiplication capacity of lymphocyte.
The multiplication capacity of lymphocyte=ConA hole OD value-control wells OD value.
The impact (n=10) on mouse lymphocyte conversion capability of table 2 enzyme composition
From table 2, comparing with matched group, each dosage group OD value is above matched group, and increases with dosage and increase, its
Middle dosage group OD value high, middle has significant difference (P ＜ 0.05 or P ＜ 0.01) compared with comparison class value.This shows the present invention's
Enzyme composition has the effect strengthening mouse lymphocyte conversion capability, i.e. has the work strengthening mouse cell immunologic function
With.
2.2 dinitrofluorobenzene (DNFB) induction delayed allergy (DTH) test
After giving each group of continuous gavage 30d of test mice by prescribed dose, mouse web portion unhairing, with 1%DNFB acetone Oleum Sesami
Solution 50 μ L smears sensitization, 5d after sensitization, and mouse right ear uniform application 1%DNFB acetone sesame oil solution 10 μ L attacks, left
Ear is smeared acetone sesame oil solution and is compared, and after attack, 24h puts to death mice, cuts left and right auricular concha, in same position cut-off footpath 8mm's
Auricle is weighed, and the difference of left and right auricle weight is as swelling.
The impact (n=10) on mice delayed allergy of table 3 enzyme composition
As shown in Table 3, compared with matched group, the low dosage of enzyme composition of the present invention is not clear on the impact of mice ear degree
Aobvious (P ＞ 0.05)；Middle dosage and high dose are obviously promoted rising effect (P ＜ 0.05) to mice ear degree.Ferment combines
Thing has facilitation to the ear swelling degree of mice delayed allergy, i.e. has the work strengthening mouse cell immunologic function
With.
2.3 antibody-producting cell detections
After giving each group of continuous gavage 30d of test mice by prescribed dose, every Mus lumbar injection 2% (v/v) hematocrit
SRBC0.2mL sensitization.After immunity 5d, mice cervical dislocation is put to death.Take out spleen, make single cell suspension, carry out haemolysis empty
Patch test.Counting hemolysis plaque number, represents antibody-producting cell number with plaque number/106 splenocyte.
The impact (n=10) on mice spleen antibody-producting cell amount of table 4 enzyme composition
As shown in Table 4, comparing with matched group, each dosage group plaque number is above matched group, and increases with dosage and increase,
Dosage group plaque number the highest, middle has significant difference (P ＜ 0.05) compared with comparison class value.This shows that thing of the present invention has increasing
The ability of strong mice spleen antibody-producting cell, i.e. has the effect strengthening mouse humoral immune function.
The mensuration of 2.4 serum hemolysins
After giving each group of continuous gavage 30d of test mice by prescribed dose, every mouse peritoneal injects 5% chicken erythrocyte suspension
0.2mL carries out immunity, and after immune 7 days, angular vein clump takes blood, and ambient temperatare is put 1 hour, is divided with tube wall by solidification blood with minute hand
From, it is centrifuged 10min with 2000r/min.Take serum normal saline dilution 100 times, take dilute serum 1mL, with 5% chicken red blood cell
Suspension 0.5mL, 10% complement (guinea pig serum is made into 10% with normal saline) 0.5mL mix, 37 DEG C of insulation 30min, 0 DEG C of end
Only reaction.After Li Xin, take supernatant spectrophotometer colorimetric at 540nm, recording light density value.
The impact (n=10) on mice serum Hemolysin formation of table 5 enzyme composition
From table 5, compared with matched group, the present invention each dosage group is all omited in the impact of mice serum Hemolysin formation
Higher than matched group, but there are no significant significant difference (P ＞ 0.05).Illustrate that enzyme composition of the present invention is to mice serum haemolysis
The generation of element does not makes significant difference.
2.5 mice carbonic clearance tests
After giving each group of continuous gavage 30d of test mice by prescribed dose, press 0.1mL/10g amount injection from the tail vein of mice
Concentration is the prepared Chinese ink of 20%, after interval 2min, 15min, takes blood 20 μ L from mice angular vein clump respectively, joins the 1% of 2mL
Na2CO3In solution.By photometer difference measured value OD value at 600nm wavelength, with Na2CO3Solution makees blank.Then
Cervical dislocation puts to death mice, takes out liver and spleen, blots the blood stains of organ surface with filter paper, weigh.By the OD value measured,
Liver and spleen weight substitute into formula, calculate phagocytic index (representing the ability of mice carbonic clearance with phagocytic index).
The impact (n=10) on mouse macrophage carbonic clearance ability of table 6 enzyme composition
From table 6, comparing with matched group, the present invention high, medium and low dosage group is to mouse macrophage carbonic clearance ability
Phagocytic index is respectively provided with obvious rising effect (P ＜ 0.05).This illustrates that thing of the present invention has the promotion phagocytal phagocytosis of mice
Effect, can strengthen its non-specific immunity.
2.6 Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell tests
After giving each group of continuous gavage 30d of test mice by prescribed dose, to every mouse peritoneal injection chicken erythrocyte suspension,
Activate mouse macrophage.After 4d, cervical dislocation puts to death mice, and lumbar injection only adds the Hank's liquid 4mL/ of calf serum,
Draw 1% chicken red blood cell mixing of abdominal cavity washing liquid and equivalent.Drawing 0.5mL mixed liquor, add in the agar circle of slide, placement is incubated
20 minutes are hatched for 37 DEG C in case.With normal saline, non-attached cell is washed out after hatching end, in methanol solution, fixes 1 minute,
Giemsa liquid dyes 15 minutes.With 40X microscopic counting phagocytic rate and phagocytic index (phagocytic rate is in every 100 macrophages,
The percentage rate shared by macrophage of phagocytosis chicken red blood cell；Phagocytic index is the individual of average each macrophage phagocytic chicken red blood cell
Number), and judge the phagocytic activity of macrophage accordingly.
Phagocytic percentage=(the phagocyte number of the phagocyte number ÷ counting of phagocytosis chicken red blood cell) × 100
The phagocyte number of the chicken red blood cell number ÷ counting of phagocytic index=swallowed
The impact (n=10) on peritoneal macrophage phagocytosis chicken red blood cell index of table 7 enzyme composition
As shown in Table 7, comparing with matched group, the high, medium and low dosage of the present invention is red carefully to Turnover of Mouse Peritoneal Macrophages phagocytosis chicken
The phagocytic rate of born of the same parents and phagocytic index are respectively provided with obvious rising effect (P ＜ 0.05).Illustrate that thing of the present invention has promotion mice and bites
The phagocytosis of cell, can strengthen its non-specific immunity.
2.7NK cytoactive detection is tested.
Give after each group of continuous gavage 30d of test mice by prescribed dose, aseptic take spleen, make individual cells suspension (cell
Concentration is 2 × 107/mL), action effect cell.Take target cell (YAC-1 cell) and each 100 μ L of effector lymphocyte (effect target ratio 50:
1), add in U-shaped 96 well culture plates；Target cell Spontaneous release hole adds target cell and each 100 μ L of culture fluid, and target cell maximum discharges
Hole adds each 100 μ L of target cell 2.5%Triton；Above-mentioned every it is all provided with three repeating holes, in 37 DEG C, 5%CO2Incubator is cultivated
Then 96 well culture plates are centrifuged 5min with 1500r/min by 4h, and every hole is drawn in 96 hole incubators at the bottom of supernatant 100 μ L horizontalization, with
Time add LDH substrate μ L at night 100, react 3min, every hole add 1mol/L HCl 30 μ L, microplate reader 490nm at mensuration light
Density value (OD), is calculated by following equation:
[(the empty OD value-Spontaneous release sky OD value of reaction)/(the empty OD-Spontaneous release of maximum release is empty for NK cytoactive %=
OD value)] 100%
The impact (n=10) on NK cells in mice activity of table 8 enzyme composition
From table 8, comparing with matched group, each dosage group NK cytoactive value is above matched group, and increases with dosage
And increase, Qi Zhonggao, middle dosage group NK cytoactive value have significant difference (P ＜ 0.05 or P ＜ 0.01) compared with matched group,
This shows that thing of the present invention has the effect strengthening NK cells in mice activity.
3, conclusion
According to the relevant enhancing immunity examination in " health food inspection and assessment technique enforcement of regulations handbook " (2003 editions)
Test result criterion, this experimental result: the enzyme composition of (1) present invention has the effect of the function promoting cellular immunization
(mouse spleen lymphocyte transformation experiment is positive with delayed allergy experimental result)；(2) the enzyme composition tool of the present invention
There is the effect (antibody-producting cell detection result of the test is positive) of the function promoting humoral immunization；(3) the ferment combination of the present invention
Thing have promote mononuclear phagocyte function effect (mice carbonic clearance experiment with Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red carefully
Born of the same parents' experimental result is all positive)；(4) enzyme composition of the present invention has the effect promoting NK cytoactive.Accordingly, it is shown that this
The enzyme composition of invention has the effect strengthening animal immunizing power.
Model case
Case 1: Zhang, female, 50 years old, medical history；Three-hypers, heart disease, dizzy unable, lumbago, leg pain, walking disorder, receive
Differ from, easily catch a cold.Taking blood pressure lowering, blood fat reducing, blood sugar lowering, Radix Salviae Miltiorrhizae drop pill etc. the most for many years, just pain of taking medicine alleviates, and continues
It is invalid to take, limitation of activity.The dialectical deficiency of vital energy, lumbago due to renal deficiency, blood stasis road blocking, after take enzyme composition of the present invention, take 20 days
After, gradually to feel strong, lumbago and skelalgia is alleviated, then takes February, and freely, orthobiosis, the number of times caught a cold reduces in walking.
Case 2: Zou, man, 17 years old, body constitution lowly, easily suffered from flu；Symptom is flu, dry cough without phlegm, Shou Zuxin
Heat, particularly dysphoria with smothery sensation at night, dry mouth and parching tongue, be hard and dry, soreness of waist night sweat.Take ferment effervescent tablet of the present invention, every day 3, often
Sheet 9g, after 5 days, cough reduces, and following the service is after 10 days, and cough disappears, and stool deliquescing, body constitution strengthen, hence it is evident that feel that body is light, its
Its malaise symptoms disappears the most therewith.
Case 3: Xue, 35 years old；Body constitution is low, easy catching a cold, to dust allergy, patient heart is depressed, poor sleeping quality, drink
Eat the best.Taking ferment effervescent tablet of the present invention, every day 3, every 9g, after 5 days, sleep quality is obviously improved, and appetite increases；Continuous clothes
After 15 days, body weight increases, and Abwehrkraft des Koepers increases, and disappears the anaphylaxis such as pollen, dust.Follow up a case by regular visits to after half a year, through looking into allergy
Former recovery from illness, is not suffering from flu.
Claims (10)
1. one kind has the enzyme composition strengthening immune function of human body, it is characterised in that this enzyme composition includes following group
Point: probiotic bacteria 1-15 weight portion, plant enzyme stock solution 10-120 weight portion and Chinese medicine extract 10-80 weight portion；Described ferment
Compositions is to mix with plant enzyme stock solution after the sterilized technique of Chinese medicine extract, and inoculate beneficial flora carry out sealed fermenting and
Become；
Described plant enzyme stock solution is fermented in the hermetic container that temperature is 30-40 DEG C by after fruit and vegerable impregnation liquid inoculation beer yeast
2-5d and obtain；Wherein beer yeast inoculum concentration is the 7-10% of fruit and vegerable stock solution weight；
Described Chinese medicine extract is extracted by following bulk drugs and forms: Radix Panacis Quinquefolii 2-10 weight portion, Semen Ginkgo 2-10 weight portion, Ganoderma 1-
8 weight portions, Radix Et Caulis Acanthopanacis Senticosi 2-10 weight portion, Cortex Eucommiae 2-8 weight portion, Caulis Spatholobi 1-8 weight portion, Herba Inulae cappae 0.5-6 weight portion, Rhizoma Curculiginis
0.5-6 weight portion, Rhizoma Cyperi 0.2-3 weight portion and Fructus Lycii 0.5-5 weight portion.
There is enzyme composition the most as claimed in claim 1 that strengthen immune function of human body, it is characterised in that described fruit and vegerable impregnate
Liquid mixed by fruit and vegetable materials, remelt sugar and pure water stand 24h after make through ozone sterilization, described fruit and vegetable materials, remelt sugar and pure
The ratio of weight and number of water is 3-4:1:6, and described fruit and vegetable materials is Fructus Pruni pseudocerasi and Fructus actinidiae chinensis.
There is enzyme composition the most as claimed in claim 1 that strengthen immune function of human body, it is characterised in that described probiotic bacteria is
Probiotic bacteria powder, is uniformly mixed by following at least two powder: bacillus bifidus 1.5-3 weight portion, lactobacillus rhamnosus 2-
2.5 weight portions, lactobacillus casei 1.2-2 weight portion, bacillus acidophilus's 1.4-2.5 weight portion and Lactobacillus plantarum 2-3 weight
Part, the viable bacteria content of described probiotic bacteria powder is 1 × 108-5×108cfu/g。
There is enzyme composition the most as claimed in claim 1 that strengthen immune function of human body, it is characterised in that described ferment combines
Thing also includes chitosan 10-50 weight portion, inulin 5-30 weight portion and vitamin B group's 12-35 weight portion.
5. enzyme composition as claimed in claim 4, it is characterised in that described enzyme composition includes following component: prebiotic
Bacterium 12.5 weight portion, plant enzyme stock solution 86 weight portion, Chinese medicine extract 40.7 weight portion, chitosan 26 weight portion, inulin 18
Weight portion and vitamin B group 22.5 weight portion；
Wherein, described Chinese medicine extract is extracted by following crude drug and forms: Radix Panacis Quinquefolii 5.8 weight portion, Semen Ginkgo 4.5 weight portion, spirit
Sesame 5 weight portion, Radix Et Caulis Acanthopanacis Senticosi 4.5 weight portion, the Cortex Eucommiae 3.2 weight portion, Caulis Spatholobi 4 weight portion, Herba Inulae cappae 3.5 weight portion, Rhizoma Curculiginis 3.5
Weight portion, Rhizoma Cyperi 1.5 weight portion and Fructus Lycii 2 weight portion.
6. one kind has the effervescent tablet strengthening immune function of human body, it is characterised in that described effervescent tablet is by claim 1 to 5
One of them described enzyme composition and adjuvant are made, and the ratio of weight and number of described compositions and adjuvant is 1:0.5-1:3.
7. effervescent tablet as claimed in claim 5, it is characterised in that described adjuvant include sodium starch glycol 35-80 weight portion,
Sodium bicarbonate 60-132 weight portion, citric acid 35-80 weight portion, maltose alcohol 10-40 weight portion, cherry syrup 30-60 weight
Part, sodium palmitate 16-52 weight portion and zinc stearate 10-30 weight portion.
8. effervescent tablet as claimed in claim 7, it is characterised in that described effervescent tablet by following enzyme composition and adjuvant through adding
Work is prepared from:
Enzyme composition: 187.7 weight portions；
Adjuvant: sodium starch glycol 64 weight portion, sodium bicarbonate 118 weight portion, citric acid 45 weight portion, maltose alcohol 28 weight
Part, cherry syrup 51 weight portion, sodium palmitate 34 weight portion and zinc stearate 20 weight portion.
9., by a preparation method for the enzyme composition described in claim 4 with enhancing immune function of human body, its feature exists
In, described method comprises the steps:
(1) preparation of Chinese medicine extract:
(1.1) first take the Semen Ginkgo of described weight portion, Ganoderma and Radix Panacis Quinquefolii to pulverize, cross 60 mesh sieves, load in extraction kettle, with
Within 15-25L/ hour, flow velocity is passed through CO2, adds 15% ethanol and makees entrainer, under the conditions of temperature holding 45 DEG C, pressure are 30MPa
Extraction 4h, carries out separating for the first time, collects crude extract, and crude extract goes successively to separating still and carries out secondary separation, at pressure
Under the conditions of being 45 DEG C for 12MPa, temperature extract 3.5h, extract is put into pillar seperator separate, obtain upper strata grease and under
The watery thing of layer；Taking off the watery thing of layer, add ethyl acetate and extract three times, separatory funnel is layered, and collects water layer, extracts three times
Water layer merges, and adds activated carbon roguing, obtained solution 1；
(1.2) Herba Inulae cappae of recipe quantity, Radix Et Caulis Acanthopanacis Senticosi, the Cortex Eucommiae, Caulis Spatholobi, Rhizoma Curculiginis, Rhizoma Cyperi and Fructus Lycii are weighed, boiling twice,
1h every time；Amount of water is add raw material gross weight 10 times for the first time；Amount of water is add raw material gross weight 8 times for the second time；Merge
Decoction liquor, filters, obtains solution 2；
(1.3) solution 2 mix homogeneously that the solution 1 step (1.1) prepared and step (1.2) prepare, obtains Chinese medicine extract；
(2) preparation of plant enzyme stock solution: added to by remelt sugar in fruit and vegetable materials, is simultaneously introduced pure water mix homogeneously, stands
After extraction 24h, carry out ozone sterilization, filter, obtain planting dip；Fruit and vegerable impregnation liquid is inoculated into the medicated beer that inoculum concentration is 8%
Yeast, ferment in the hermetic container that temperature is 30-40 DEG C 3d, obtains plant enzyme stock solution；
(3) Chinese medicine extract and plant enzyme stock solution are mixed, add probiotic bacteria, chitosan, inulin and vitamin B group, in temperature
Degree is the 5-7d that ferments in the hermetic container of 30-40 DEG C, filters, collects filtrate, and filtrate carries out low temperature spray drying, obtains powdery
Enzyme composition.
10. one kind by the preparation method of effervescent tablet described in claim 8, it is characterised in that described method comprises the steps:
A. prepare the enzyme composition of powdery, weigh formula ratio and cross 60 mesh sieves, stand-by；
B. the sodium starch glycol and the maltose alcohol that weigh formula ratio put into baking oven, dry 3h, mistake after cooling under the conditions of 60 DEG C
60 mesh sieves, stand-by；
C. weigh the sodium bicarbonate of formula ratio, dissolve with the ethanol solution of 60-80%, add the ferment that step a of 1/2 weight processes
Step b of promotor composition and 1/2 weight processes sodium starch glycol and maltose alcohol, makes soft material, wet granulation, and low temperature is done
Dry, granulate, obtain bicarbonate soda granule；
D. the citric acid weighing formula ratio is mixed homogeneously with cherry syrup, dissolves with the ethanol solution of 60-80%, adds 1/2 weight
Amount step a process enzyme composition and 1/2 weight step b process sodium starch glycol and maltose alcohol, make
Soft material, wet granulation, cold drying, granulate, obtain granulates；
Granulates mixing prepared by the bicarbonate soda granule e. step c prepared and step d, adds sodium palmitate and stearic acid
Zinc uniformly mixes, tabletting, prepares effervescent tablet.
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| CN106880040A (en) * | 2017-04-11 | 2017-06-23 | 姜红成 | Prepare the fermentation composition and preparation method with the plant enzyme suitable for the conditioning maintenance of rheumatism osteoarthritis patients |
| CN106962937A (en) * | 2017-04-11 | 2017-07-21 | 姜红成 | Prepare fermentation composition and preparation method with prevention conditioning hypertension, high fat of blood, hyperglycaemia and routine servicing effect plant enzyme |
| CN106974279A (en) * | 2017-04-11 | 2017-07-25 | 姜红成 | Prepare the fermentation composition and preparation method for being applied to cardiovascular and cerebrovascular diseases conditioning and its routine servicing effect plant enzyme |
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| CN112841661A (en) * | 2021-02-01 | 2021-05-28 | 贵州菊春堂农业科技发展有限公司 | Preparation method of chrysanthemum prebiotics |
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Application publication date: 20161109 |