CN106174484A - There is the enzyme composition of cough and asthma relieving efficacy, effervescent granule and preparation method - Google Patents
There is the enzyme composition of cough and asthma relieving efficacy, effervescent granule and preparation method Download PDFInfo
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- CN106174484A CN106174484A CN201610541052.9A CN201610541052A CN106174484A CN 106174484 A CN106174484 A CN 106174484A CN 201610541052 A CN201610541052 A CN 201610541052A CN 106174484 A CN106174484 A CN 106174484A
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Abstract
The present invention relates to a kind of enzyme composition with cough and asthma relieving efficacy, this enzyme composition includes following component: probiotic bacteria 0.1 15 weight portion, plant enzyme stock solution 10 120 weight portion and Chinese medicine extract 10 80 weight portion；Enzyme composition is to mix with plant enzyme stock solution after the sterilized technique of Chinese medicine extract, and inoculates beneficial flora and carry out sealed fermenting and form；Chinese medicine extract is extracted by the crude drug of following weight portion and forms: Herba Portulacae 2 10 parts, Herba Ardisiae Japonicae 2 10 parts, Herba Asari 18 parts, Fructus Momordicae 2 10 parts, Radix Rhodiolae 28 parts, Fructus Citri tangerinae core 18 parts, slurry grass 0.5 6 parts, Herba Menthae 0.5 6 parts, Caulis Mahoniae 0.5 6 parts and Pericarpium Citri Reticulatae 0.5 5 parts.Probiotic bacteria and functional oligose are used in combination by this enzyme composition, coordinate effect of Chinese medicine extract relieving cough and asthma, it is adaptable to cough with copious phlegm, fullness in the chest and shortness of breath, and dry pharynx is had sore throat, the patient of tracheitis.
Description
Technical field
The invention belongs to field of health care food, particularly to a kind of, there is the enzyme composition of cough and asthma relieving efficacy, combination
Effervescent granule prepared by thing and preparation method.
Background technology
Ferment is in recent years to rise in Japan, a kind of functional food of TaiWan, China, and it is with fermented by mixed bacterium such as yeast
The plant fermented liquid of more than 50 kinds fruit and vegetable gained.Ferment is rich in the functional cometabolism from more than 50 kinds fruit and vegetable materials
Product, such as polyphenol and oligosaccharide.The at present research to fruit-vegerable ferment focuses mostly on adjusting and improving at raw material, strain and fermentation technology
Aspect, also occurs in that the health food that some add the ferment of probiotic bacterias and prepared by ferment simultaneously.Probiotics fermention fruit and vegerable, can
Activate the effective efficiency composition in fruit and vegerable, active effect material of dissolution more elite.As number of patent application is
201410087224.0 patent application document disclosed in a kind of protecting liver and expelling toxin fruit-vegerable ferment and preparation method thereof；And for example patent Shen
Please number be 201610108666.8 patent application document disclosed in a kind of fruit and vegerable active ferment liquid, by by multiple sarcocarp and vegetables
Dish sealed fermenting prepares, and enzyme liquid is the multi-nutrition maintaining human body in a basic balance, improves body immunity.But, above-mentioned ferment
Element composition is simple, and not having effect of relieving cough and asthma, ferment product is fruit and vegerable fermentation liquid, and fluid product is unfavorable for carrying transport.
Inadequate to the concern of mouthfeel, quality and the dosage form of ferment finished product for present stage, applicant feels the need to into one
The Exploitative potential of step exploitation ferment, to obtain the ferment based food that mouthfeel is adjustable controlled and is convenient for carrying.
Summary of the invention
Present invention seek to address that technical problem present in prior art.To this end, it is an object of the present invention to propose
A kind of enzyme composition with cough and asthma relieving efficacy, probiotic bacteria and functional oligose are used in combination by this enzyme composition,
And add Chinese medicine ingredients, make said composition while having good intestinal microbial population regulatory function, it is possible to promote little molecule
Sugar and polysaccharide are to the conversion of oligosaccharide；Give full play to effect of the Chinese medicine extract relieving cough and asthma of enzyme composition, it is adaptable to cough
Coughing abundant expectoration, fullness in the chest and shortness of breath, dry pharynx has sore throat, the patient of tracheitis.
Technical scheme is as follows:
A kind of enzyme composition with cough and asthma relieving efficacy, this enzyme composition includes following component: probiotic bacteria 0.1-
15 weight portions, plant enzyme stock solution 10-120 weight portion and Chinese medicine extract 10-80 weight portion；Described enzyme composition is Chinese medicine
Mix with plant enzyme stock solution after the sterilized technique of extract, and inoculate beneficial flora and carry out sealed fermenting and form；
Described plant enzyme stock solution by fruit and vegerable impregnation liquid inoculation beer yeast after in the hermetic container that temperature is 30-40 DEG C
Fermentation 2-5d and obtain；Wherein beer yeast inoculum concentration is the 7-10% of fruit and vegerable stock solution weight；
Described Chinese medicine extract by following bulk drugs extract form: Herba Portulacae 2-10 weight portion, Herba Ardisiae Japonicae 2-10 weight portion,
Herba Asari 1-8 weight portion, Fructus Momordicae 2-10 weight portion, Radix Rhodiolae 2-8 weight portion, Fructus Citri tangerinae core 1-8 weight portion, slurry grass 0.5-6 weight
Part, Herba Menthae 0.5-6 weight portion, Caulis Mahoniae 0.5-6 weight portion and Pericarpium Citri Reticulatae 0.5-5 weight portion.
Chinese medicine extraction of the present invention has thing by kinds of traditional Chinese medicines compatibility, have antitussive, reduce phlegm, effect of Dingchuan.Probiotic bacteria
Coordinate fermentation with plant enzyme stock solution, the effective efficiency composition in fruit and vegerable can be activated, active effect material of dissolution more elite.With
Time use with Chinese medicine extract reasonable combination so that it is and between probiotics fermention thing, there is mutual promoting action, it is possible to double right
The regulatory function of intestinal microbial population, improves the absorption to Effective Component of Chinese Medicine, realizes effect of relieving cough and asthma further.Additionally, add
The plant enzyme stock solution added is carried out primary fermentation technique by fruit and vegerable impregnation liquid inoculation beer yeast, improves ferment of the present invention combination
Thing, from fermentability, is that in enzyme composition, each effective ingredient can be discharged more fully.
Further reasonable disposition fruit and vegerable impregnation liquid, described fruit and vegerable impregnation liquid is mixed by fruit and vegetable materials, white sugar and pure water
Making through ozone sterilization after standing 24h, the ratio of weight and number of described fruit and vegetable materials, white sugar and water is 3-4:1:6, and described fruit and vegerable are former
Material is pears, Folium Eriobotryae and Fructus Pruni.
Further, described probiotic bacteria is probiotic bacteria powder, following at least two powder be uniformly mixed: bifid bar
Bacterium 1.5-3 weight portion, lactobacillus rhamnosus 2-2.5 weight portion, lactobacillus casei 1.2-2 weight portion, bacillus acidophilus 1.4-2.5
Weight portion and Lactobacillus plantarum 2-3 weight portion, the viable bacteria content of described probiotic bacteria powder is 1 × 108-5×108cfu/g。
The functional oligose added in enzyme composition of the present invention is growth of probiotics substrate, has and promotes probiotic bacteria
The physiological function of propagation, the further multiplication regulatory function to intestinal microbial population, thus effect based on intestinal microbial population plays more preferably
Enhancing immunity；In concrete scheme, described enzyme composition also includes stachyose 10-50 weight portion and vitamin B group
12-35 weight portion.
In further preferred version, this enzyme composition includes following component: probiotic bacteria 12.5 weight portion, plant ferment
Element stock solution 86 weight portion, Chinese medicine extract 40.7 weight portion, stachyose 26 weight portion and vitamin B group 22.5 weight portion；
Wherein, described Chinese medicine extract is extracted by following crude drug and forms: Herba Portulacae 5.8 weight portion, Herba Ardisiae Japonicae 4.5 weight
Part, Herba Asari 5 weight portion, Fructus Momordicae 4.5 weight portion, Radix Rhodiolae 3.2 weight portion, Fructus Citri tangerinae core 4 weight portion, starch careless 3.5 weight portions,
Herba Menthae 3.5 weight portion, Caulis Mahoniae 3.2 weight portion and Pericarpium Citri Reticulatae 2 weight portion.
For improving property easy to use and the storage time limit of enzyme composition, present invention also offers one and there is relieving cough and asthma
The effervescent granule of effect, effervescent granule is made up of above-mentioned enzyme composition and adjuvant, described compositions and the parts by weight of adjuvant
Ratio is 1:0.5-1:3.
Wherein, described adjuvant includes pregelatinized starch 35-80 weight portion, polyethylene glycol 6000 15-40 weight portion, bicarbonate
Sodium 60-132 weight portion and tartaric acid 35-80 weight portion, D-xylose 10-40 weight portion, Thomas balsam 30-60 weight portion and
Methylcellulose 16-52 weight portion.By being used in conjunction with of pregelatinized starch, D-xylose and Thomas balsam, ferment group can be improved
The availability of compound, assists compositions or strengthens compositions effect, improving compositions curative effect；D-xylose also has taste masking work simultaneously
With.The slightly solubility caused based on Chinese medicine macromolecular substances, the present invention uses by pregelatinized starch and methylcellulose are collaborative, enters
One step improves the melting of effervescent granule, makes granule dissolve more complete.
Design learns, in effervescent granule of the present invention during the following scheme of each proportioning raw materials, have relieving cough and asthma optimal after deliberation
Curative effect, and effervescent granule stability, molding and bioavailability are the most optimal.Concrete, effervescent granule is by following enzyme composition
Processed with adjuvant it is prepared from:
Enzyme composition: 187.7 weight portions；
Adjuvant: pregelatinized starch 64 weight portion, polyethylene glycol 6000 23 weight portion, sodium bicarbonate 118 weight portion, winestone
Acid 45 weight portions, D-xylose 28 weight portion, Thomas balsam 51 weight portion and methylcellulose 34 weight portion.
It is a further object to provide the preparation method of a kind of above-mentioned enzyme composition, described method includes following
Step:
(1) preparation of Chinese medicine extract:
(1.1) take the Herba Asari of described weight portion, Herba Ardisiae Japonicae, Fructus Citri tangerinae core and slurry grass and carry out soak by water 3 times, 15 times amount for the first time
Soak by water 2-4h, for the second time 12 times amount soak by water 1-1.5h, for the third time 10 times amount soak by water 1-1.5h, merging filtrate, temperature is
Being concentrated into relative density under the conditions of 45 DEG C is 1.00-1.15g/ml, cooling, adds 50-60% ethanol, stands overnight, and filters, filtrate
Under the conditions of temperature is 50-60 DEG C, it is concentrated into the thick paste that relative density is 1.00-1.25g/ml, adds water to total amount 50-70%, quiet
Put 30-40h, take supernatant, obtain solution 1；
(1.2) Herba Portulacae of recipe quantity, Fructus Momordicae, Radix Rhodiolae, Herba Menthae, Caulis Mahoniae and Pericarpium Citri Reticulatae are weighed, boiling twice,
1h every time；Amount of water is add raw material gross weight 10 times for the first time；Amount of water is add raw material gross weight 8 times for the second time；Merge
Decoction liquor, filters, obtains solution 2；
(1.3) solution 2 mix homogeneously that the solution 1 step (1.1) prepared and step (1.2) prepare, obtains Chinese medicine extraction
Thing；
(2) preparation of plant enzyme stock solution: added to by white sugar in fruit and vegetable materials, is simultaneously introduced pure water mix homogeneously,
After standing extraction 24h, carry out ozone sterilization, filter, obtain planting dip；It is fruit and vegerable stock solution by fruit and vegerable impregnation liquid inoculation inoculum concentration
The beer yeast of the 8% of weight, ferment in the hermetic container that temperature is 30-40 DEG C 3d, obtains plant enzyme stock solution；
(3) Chinese medicine extract and plant enzyme stock solution are mixed, add probiotic bacteria, stachyose and vitamin B group, in temperature
For the 5-7d that ferments in the hermetic container of 30-40 DEG C, filtering, collect filtrate, filtrate carries out low temperature spray drying, obtains the ferment of powdery
Promotor composition.
The raw material of Chinese medicine extracted by above-mentioned steps, the extraction ratio of Effective Component of Chinese Medicine is high.Further, by above-mentioned steps system
Standby enzyme composition, improves directly interpolation probiotic bacteria and prepares the traditional handicraft of plant enzyme, and enzyme composition self tool
There is nutritional labeling splendid in curative effect medically and dietetic therapy；It also offers a kind of brand-new enzyme preparation, by this ferment
Compositions is prepared as various preparation, it is simple to carries and eats.
In preparation prepared by enzyme composition of the present invention, being preferably prepared into effervescent granule, being directly dissolved in water can make
With, and rate of dissolution is very fast, need not be by the most available sufficiently release of mixing plant effective ingredient in the effective process time.
Concrete preparation method is as follows:
A. prepare the enzyme composition of powdery, weigh formula ratio and cross 60 mesh sieves, stand-by；
B. baking oven put into by the pregelatinized starch and the D-xylose that weigh formula ratio, dries 3h, mistake after cooling under the conditions of 60 DEG C
60 mesh sieves, stand-by；
C. weigh the sodium bicarbonate of formula ratio, dissolve with the ethanol solution of 60-80%, add the step a process of 1/2 weight
Enzyme composition and 1/2 weight step b process pregelatinized starch and D-xylose, make soft material, wet granulation, low temperature
It is dried, granulate, obtains bicarbonate soda granule；
D. weigh the tartaric acid polyethylene glycol 6000 embedding after melting of formula ratio, cooling crush, cross 60 mesh sieves；
The ethanol solution of the tartaric acid 60-80% after e. step d being processed dissolves, and adds the step a process of 1/2 weight
Enzyme composition and the pregelatinized starch that processes of step b of 1/2 weight and D-xylose, make soft material, wet granulation, dry
Dry, granulate, obtain granulates；
Granulates mixing prepared by the bicarbonate soda granule f. step c prepared and step e, adds methylcellulose equal
Even mixing, crosses 80 mesh sieves, pelletizes, and cold drying arranges, and prepares effervescent granule.
The Chinese medicinal components added in the enzyme composition of the present invention has its physiologically active and health-care effect, is benefit simultaneously
Raw bacterium supplements the nutrients in fermentation, has a higher practical value, and the Chinese medicine ingredients of the present invention can effectively relieving cough and asthma, QI invigorating
Enrich blood；Probiotic bacteria, functional oligose.Between vitamin B group and each component of Chinese medicine, compatibility is reasonable, and clear curative effect has association
Effect with potentiation.
This ferment class effervescent granule is convenient for carrying, and wherein retains the Chinese medicine extract effective ingredient with relieving cough and asthma, also
Remain ferment polyphenol, and coordinate oligosaccharide to use the contents of saccharide reduced in ferment, improve products taste, and can be according to need
Regulate；Effervescent granule dosage form further improves the stability of ferment polyphenol.This enzyme composition and effervescent granule thereof are fitted
The preferably people of asthma and cough uses, and takes as daily beverage, improves internal metabolism from the angle effected a permanent cure, improves human immunity simultaneously
Power etc., it is achieved the purpose of health care.
Additionally, the technical process that plant enzyme solid preparation is prepared in enzyme composition directly interpolation is simple, it is easy to accomplish big
Large-scale production, and safe preparation process, have good application prospect.
The source of the raw material of Chinese medicine composition of the present invention:
Herba Portulacae: this product is portulacaceous plant Herba Portulacae Portulaca oleracea L., with all herbal medicine.
Herba Ardisiae Japonicae: for the Herb of Myrsinacea plant Herba Ardisiae Japonicae Ardisia japonica (Hornst.) B1..
Herba Asari: this product is aristolochiaceae plant Herba Asari Asarum heterotropoides Fr.Schmidt
Var.mandshuricum (Maxim.) Kitag., Seoul Herba Asari Asarum sieboldii Miq.var.seoulense
Nakai or the dry herb of China Herba Asari Asarum sieboldii Miq..
Fructus Momordicae: for the fruit of cucurbitaceous plant Fructus Momordicae.
Radix Rhodiolae: Crassulaceae Rhodida plant Radix Rhodiolae Rhodiola saera (Prain) Fu, with all herbal medicine.
Fructus Citri tangerinae core: have another name called Semen Citri Reticulatae, dry for rutaceae orange Citrus reticulata Blanco and variety thereof
Dry mature seed.
Slurry grass: the herb of Oxalidaceae plant Herba Oxalidis Corniculatae Oxalis corniculata L..
Herba Menthae: this product is the dry aerial parts of Labiatae mint Herba Menthae Mentha haplocalyx Briq..
Caulis Mahoniae: for Berberidaceae plant Mahonia bealei (Fort.) Carr., mahonia fortuneiFedde or the stem of south China Mahonia fortunei (Lindl.) Fedde.
Pericarpium Citri Reticulatae: this product is rutaceae orange Citrus reticulata Blanco and the drying and ripening of variety thereof
Peel.
Detailed description of the invention
Embodiment 1 enzyme composition
The prescription of the enzyme composition of the present embodiment is:
Probiotic bacteria 40g, plant enzyme stock solution 100g, Chinese medicine extract 100g；
Wherein, probiotic bacteria is made up of following probiotic bacteria powder: bacillus bifidus 15g, lactobacillus rhamnosus 25g；
Plant enzyme stock solution is prepared from by following raw material: pears 10g, Folium Eriobotryae 10g, Fructus Pruni 10g, white sugar 10g, pure water
60g, beer yeast 7g；
Chinese medicine extract is prepared from by following raw material: Herba Portulacae 40g, Herba Ardisiae Japonicae 40g, Herba Asari 20g, and Fructus Momordicae 40g is red
Herba hylotelephii erythrosticti 40g, Fructus Citri tangerinae core 20g, slurry grass 10g, Herba Menthae 10g, Caulis Mahoniae 10g, Pericarpium Citri Reticulatae 10g.
Wherein, plant enzyme stock solution preparation method is: select maturation, without rot pears, Folium Eriobotryae and Fructus Pruni, epidermis clean, belt leather
Stripping and slicing, addition white sugar and pure water, mix homogeneously, after standing 24h, ozone sterilization, prepare plant extraction liquid；Inoculation beer yeast
To plant extraction liquid, ferment in the hermetic container of temperature 30-40 DEG C 2d, obtains plant enzyme stock solution.
Embodiment 2 enzyme composition
The prescription of the enzyme composition of the present embodiment is:
Probiotic bacteria 150g, plant enzyme stock solution 1200g, Chinese medicine extract 800g；
Wherein, probiotic bacteria is made up of following probiotic bacteria powder: lactobacillus rhamnosus 50g, lactobacillus casei 100g；
Plant enzyme stock solution is prepared from by following raw material: pears 200g, Folium Eriobotryae 100g, Fructus Pruni 133g, and white sugar 108g is pure
Water 650g, beer yeast 119g；
Chinese medicine extract is prepared from by following raw material: Herba Portulacae 200g, Herba Ardisiae Japonicae 200g, Herba Asari 160g, Fructus Momordicae
200g, Radix Rhodiolae 160g, Fructus Citri tangerinae core 160g, slurry grass 120g, Herba Menthae 120g, Caulis Mahoniae 120g, Pericarpium Citri Reticulatae 100g.
Wherein, plant enzyme stock solution preparation method is: select maturation, without rot pears, Folium Eriobotryae and Fructus Pruni, epidermis clean, belt leather
Stripping and slicing, addition white sugar and pure water, mix homogeneously, after standing 24h, ozone sterilization, prepare plant extraction liquid；Inoculation beer yeast
To plant extraction liquid, ferment in the hermetic container of temperature 30-40 DEG C 5d, obtains plant enzyme stock solution.
Embodiment 3 enzyme composition
The prescription of the enzyme composition of the present embodiment is:
Probiotic bacteria 125g, plant enzyme stock solution 860g, Chinese medicine extract 407g, stachyose 260g and vitamin B group
225g；
Wherein, probiotic bacteria is made up of following probiotic powder: bifid bar 50g, lactobacillus casei 50g, Lactobacillus plantarum 25g；
Plant enzyme stock solution is prepared from by following raw material: pears 150g, Folium Eriobotryae 150g, Fructus Pruni 150g, and white sugar 150g is pure
Water 900g, beer yeast 115g；
Described Chinese medicine extract is extracted by following crude drug and forms: Herba Portulacae 116g, Herba Ardisiae Japonicae 90g, Herba Asari 100g, arhat
Really 90g, Radix Rhodiolae 64g, Fructus Citri tangerinae core 80g, slurry grass 70g, Herba Menthae 70g, Caulis Mahoniae 64g and Pericarpium Citri Reticulatae 40g；
Preparation method:
(1) preparation of Chinese medicine extract:
(1.1) take the Herba Asari of described weight portion, Herba Ardisiae Japonicae, Fructus Citri tangerinae core and slurry grass and carry out soak by water 3 times, 15 times amount for the first time
Soak by water 2-4h, for the second time 12 times amount soak by water 1-1.5h, for the third time 10 times amount soak by water 1-1.5h, merging filtrate, temperature is
Being concentrated into relative density under the conditions of 45 DEG C is 1.00-1.15g/ml, cooling, adds 50-60% ethanol, stands overnight, and filters, filtrate
Under the conditions of temperature is 50-60 DEG C, it is concentrated into the thick paste that relative density is 1.00-1.25g/ml, adds water to total amount 50-70%, quiet
Put 30-40h, take supernatant, obtain solution 1；(1.2) Herba Portulacae of recipe quantity, Fructus Momordicae, Radix Rhodiolae, Herba Menthae, Caulis Mahoniae are weighed
And Pericarpium Citri Reticulatae, boiling twice, each 1h；Amount of water is add raw material gross weight 10 times for the first time；Amount of water is for adding for the second time
Enter 8 times of raw material gross weight；Merge decoction liquor, filter, obtain solution 2；(1.3) solution 1 step (1.1) prepared and step
(1.2) solution 2 mix homogeneously prepared, obtains Chinese medicine extract, takes 407g standby；
(2) preparation of plant enzyme stock solution: select maturation, without rot pears, Folium Eriobotryae and Fructus Pruni, epidermis clean, belt leather is cut
Block, addition white sugar and pure water, mix homogeneously, after standing 24h, ozone sterilization, prepare plant extraction liquid；Inoculation beer yeast is extremely
Plant extraction liquid, ferment in the hermetic container of temperature 30-40 DEG C 4d, obtains plant enzyme stock solution, takes 860g standby；
(3) Chinese medicine extract and plant enzyme stock solution are mixed, add probiotic bacteria, stachyose and vitamin B group, in temperature
For the 5-7d that ferments in the hermetic container of 30-40 DEG C, filtering, collect filtrate, filtrate carries out low temperature spray drying, obtains the ferment of powdery
Promotor composition.
Embodiment 4 enzyme composition
The prescription of the enzyme composition of the present embodiment is:
Probiotic bacteria 20g, plant enzyme stock solution 450g, Chinese medicine extract 200g, stachyose 500g and vitamin B group
120g；
Wherein, probiotic bacteria is made up of following probiotic powder: bacillus bifidus 8g, lactobacillus rhamnosus 7g, bacillus acidophilus 5g；
Plant enzyme stock solution is prepared from by following raw material: pears 40g, Folium Eriobotryae 40g, Fructus Pruni 55g, white sugar 45g, pure water
270g, beer yeast 40g；
Described Chinese medicine extract is extracted by following crude drug and forms: Herba Portulacae 70g, Herba Ardisiae Japonicae 60g, Herba Asari 40g, Fructus Momordicae
10g, Radix Rhodiolae 50g, Fructus Citri tangerinae core 40g, slurry grass 20g, Herba Menthae 20g, Caulis Mahoniae 20g and Pericarpium Citri Reticulatae 20g.
Preparation method: prepare according to the method for embodiment 3.
Embodiment 5 effervescent granule
Use the enzyme composition 1877g of embodiment 3；
Adjuvant: pregelatinized starch 640g, polyethylene glycol 6000 230g, sodium bicarbonate 1180g, tartaric acid 450g, D-xylose
280g, Thomas balsam 510g, methylcellulose 340g.
Preparation method comprises the steps:
A. prepare the enzyme composition of powdery, weigh formula ratio and cross 60 mesh sieves, stand-by；
B. baking oven put into by the pregelatinized starch and the D-xylose that weigh formula ratio, dries 3h, mistake after cooling under the conditions of 60 DEG C
60 mesh sieves, stand-by；
C. weigh the sodium bicarbonate of formula ratio, dissolve with the ethanol solution of 60-80%, add the step a process of 1/2 weight
Enzyme composition and 1/2 weight step b process pregelatinized starch and D-xylose, make soft material, wet granulation, low temperature
It is dried, granulate, obtains bicarbonate soda granule；
D. weigh the tartaric acid polyethylene glycol 6000 embedding after melting of formula ratio, cooling crush, cross 60 mesh sieves；
E. the tartaric acid after step d being processed is mixed homogeneously with Thomas balsam, dissolves with the ethanol solution of 60-80%, adds
Enter the enzyme composition of the step a process of 1/2 weight and the pregelatinized starch of the step b process of 1/2 weight and D-xylose, system
Become soft material, wet granulation, cold drying, granulate, obtain granulates；
Granulates mixing prepared by the bicarbonate soda granule f. step c prepared and step e, adds methylcellulose equal
Even mixing, crosses 80 mesh sieves, pelletizes, and is dried, and arranges, and prepares effervescent granule.
Embodiment 6 effervescent granule
Use the enzyme composition 1870g of embodiment 4；
Adjuvant: pregelatinized starch 350g, polyethylene glycol 6000 150g, sodium bicarbonate 600g, tartaric acid 350g, D-xylose
100g, Thomas balsam 300g, methylcellulose 160g.
Preparation method: prepare according to preparation method described in embodiment 5.
Embodiment 7 effervescent granule
Use the enzyme composition 2250g of embodiment 3；
Adjuvant: pregelatinized starch 800g, polyethylene glycol 6000 400g, sodium bicarbonate 1320g, tartaric acid 800g, D-xylose
400g, Thomas balsam 600g, methylcellulose 520g.
Preparation method: prepare according to preparation method described in embodiment 5.
Embodiment 8 effervescent granule
Use the enzyme composition 5100g of embodiment 3；
Adjuvant: pregelatinized starch 780g, polyethylene glycol 6000 340g, sodium bicarbonate 1220g, tartaric acid 800g.
Preparation method comprises the steps:
A. prepare the enzyme composition of powdery, weigh formula ratio and cross 60 mesh sieves, stand-by；
B. the pregelatinized starch weighing formula ratio puts into baking oven, dries 3h under the conditions of 60 DEG C, crosses 60 mesh sieves, treat after cooling
With；
C. weigh the sodium bicarbonate of formula ratio, dissolve with the ethanol solution of 60-80%, add the step a process of 1/2 weight
Enzyme composition and the pregelatinized starch that processes of step b of 1/2 weight, make soft material, wet granulation, be dried, granulate,
To bicarbonate soda granule；
D. weigh the tartaric acid polyethylene glycol 6000 embedding after melting of formula ratio, cooling crush, cross 60 mesh sieves；
The ethanol solution of the tartaric acid 60-80% after e. step d being processed dissolves, and adds the step a process of 1/2 weight
Enzyme composition and the pregelatinized starch that processes of step b of 1/2 weight, make soft material, wet granulation, be dried, granulate,
To granulates；
Granulates prepared by the bicarbonate soda granule f. step c prepared and step e uniformly mixes, and crosses 80 mesh sieves, system
Grain, is dried, and arranges, and prepares effervescent granule.
Comparative examples 1 effervescent granule
Use the enzyme composition of embodiment 3: 1877g；
Adjuvant: pregelatinized starch 640g, polyethylene glycol 6000 230g, sodium bicarbonate 1180g, tartaric acid 450g, tell LUXIANG
Fat 510g, methylcellulose 340g.
Preparation method: in addition to being not added with D-xylose, all prepares according to preparation method described in embodiment 5.
Comparative examples 2 effervescent granule
Use the enzyme composition 1877g of embodiment 3；
Adjuvant: pregelatinized starch 640g, polyethylene glycol 6000 230g, sodium bicarbonate 1180g, tartaric acid 450g, methyl is fine
Dimension element 340g.
Preparation method: in addition to being not added with D-xylose and Thomas balsam, all prepares according to preparation method described in embodiment 5.
Comparative examples 3 effervescent granule
Use the enzyme composition of embodiment 3: 1877g；
Adjuvant: pregelatinized starch 640g, polyethylene glycol 6000 230g, sodium bicarbonate 1180g, tartaric acid 450g, D-xylose
280g, Thomas balsam 510g.
Preparation method: in addition to being not added with methylcellulose, all prepares according to preparation method described in embodiment 5.
Comparative examples 4 effervescent granule
Use the enzyme composition of embodiment 3: 1877g；
Adjuvant: identical with adjuvant described in embodiment 5 and consumption thereof.
Preparation method comprises the steps:
A. prepare the enzyme composition of powdery, weigh formula ratio and cross 60 mesh sieves, stand-by；
B. baking oven put into by the pregelatinized starch and the D-xylose that weigh formula ratio, dries 3h, mistake after cooling under the conditions of 60 DEG C
60 mesh sieves, stand-by；
C. weighing the sodium bicarbonate of formula ratio, the enzyme composition and the formula ratio that add the process of formula ratio step a are pre-solidifying
Amylopectin and D-xylose, mix homogeneously, it is dried, granulate, obtains bicarbonate soda granule；
D. weigh the tartaric acid polyethylene glycol 6000 embedding after melting of formula ratio, cooling crush, mix with Thomas balsam
Close uniformly, cross 60 mesh sieves, granulate, obtain granulates；
Granulates mixing prepared by the bicarbonate soda granule g. step c prepared and step d, adds methylcellulose equal
Even mixing, tabletting, prepare effervescent granule.
Test example 1 mouthfeel is tested
The sample 10g of Example 3, embodiment 5, matched group embodiment 1 and comparative examples 2 so that it is be dissolved in 200ml's
In water, stirring, prepare need testing solution；Allow 10 professional test persons taste need testing solution, compare and take rear mouthfeel；Scoring mark
Accurate point of 4 grades, mouthfeel is poor, with bitter taste, 1 point；Mouthfeel is poor, with slight bitter taste 2 points；Good in taste, without bitter
Astringent taste road, 3 points；In good taste, taste is sweet, without bitter taste 4 points；Test result is shown in Table 1；
The sensory test result of table 1 enzyme composition and effervescent granule
From table 1 it follows that the adjuvant of the embodiment of the present invention 5 formula preferably improves the mouthfeel of effervescent granule, reduce medicine
The bitter taste of thing；Compared with comparative examples 1, it is known that the interpolation of D-xylose effectively carries out taste masking to effervescent granule；Enter one
Knowable to step compares with comparative examples 2, when D-xylose and the collaborative use of Thomas balsam, taste masking best results.Simultaneously from embodiment 1
Result can draw, though the enzyme composition mouthfeel of the present invention not as other groups good, but still be more people acceptance.
Test example 2 melting
With reference to the regulation in 2010 editions annex I N granules of Chinese Pharmacopoeia, ferment effervescent granule is carried out melting detection,
Detection method is as follows:
Taking test sample 10g, add hot water 200ml, stir 5min, granule should all dissolve or slight haze, but must not have different
Thing.
The effervescent granule conduct of the enzyme composition of Example 3, embodiment 5, comparative examples 3 and comparative examples 4
Test sample, carries out melting test by said method, and result of the test is shown in Table 2；
The melting result of the test of table 2 enzyme composition and effervescent granule
As can be seen from Table 2, the enzyme composition powder melting of the embodiment of the present invention 3 is the best, the granule tool of embodiment 5
Having good melting, melting is higher than comparative examples 3, it follows that the addition of pregelatinized starch and methylcellulose can be entered
One step improves the melting of effervescent granule, makes granule dissolve more complete；When lacking methylcellulose, the melting of granule shows
Write and decline.Additionally, the melting of comparative examples 4 is the best, it follows that effervescent granule prepared by the preparation method of embodiment 5
Melting is more preferably.
Test example 3 slaking test
With reference to the regulation of effervescent granule slaking test under 2010 editions annex of Chinese Pharmacopoeia, to the embodiment of the present invention 5 and comparison
The effervescent granule of embodiment 3 carries out disintegrative detection, and detection method is as follows:
Example 5 effervescent tablet 12 is for for examination group, and comparative examples 3 effervescent tablet 12 is matched group, and every weight is equal
For 3g, adding normal-temperature water 200ml, stand 30min, complete disintegrate, solution clarify (showing to dissolve completely), nothing is muddy, taste is fragrant.Examination
Testing result is；
For examination group: all effervescent tableies complete disintegrate in 3.5min, solution can be clarified and (show to have dissolved in normal-temperature water
Entirely), without muddy, taste fragrance.
Matched group: effervescent tablet is complete disintegrate in 10min, slightly cloudy, taste fragrance.
More than test shows, effervescent tablet preparation method of the present invention is mixed by substep, the preparation of mixing after substep soft material processed
Effervescent tablet disintegration prepared by method is better, dissolution can discharge the property of medicine rapidly, has taking convenience, dissolution rapid release spy
Property, reach intended galenic pharmacy effect.
Test example 4 zoology test
One, test material
(1) medicine and material
1, test medicine: enzyme composition of the present invention is tested with antibacterial action.
2, positive control drug: the spirit of compound recipe cough with asthma is tested with bacteriostasis.
3, instrument medicine: acetyloxide choline, Shanghai biochemical research is produced；
Hydrochloric acid histamine, Shanghai biochemical research is produced.
(2) animal: random hybrid Cavia porcellus, Kun Ming mice.
Two, content of the test method and result
(1) antiasthmatic effect test
1, screening animal: taking the Cavia porcellus of body weight 180～200g, male and female are also used.Being respectively put into volume is glass airtight for 4L
In cover after peace and quiet, start air compressor, spray into the salt of 2% acetylcholine and 0.1% with the constant voltage of 53KPa (400mm Hg)
After equivalent mixed liquor (draw and breathe heavily medicinal liquid) the 15S spraying of acid histamine stops, in observing 6min, there is the latent of asthmatic tic in Cavia porcellus
Fu Qi, the asthmatic latent period of Cavia porcellus person in 120S select for experiment, need not more than 120S person's reject.
2, formal test: take screening qualified Cavia porcellus 50, be randomly divided into five groups, often group 10, basic, normal, high three dosage
Test medicine group, positive controls and blank group.In be administered before surveyed respectively each group of every Cavia porcellus asthmatic latent period and
Suffocated by dyspnea the number of animals (i.e. occur twitch number of animals) fallen.
Packet situation is:
Low dose group: the enzyme composition of gavage embodiment 3, dosage is 2g/kg；
Middle dosage group: the enzyme composition of gavage embodiment 3, dosage is 4g/kg；
High dose group: the enzyme composition of gavage embodiment 3, dosage is 6g/kg；
Positive controls: gavage compound recipe cough and asthma capsule agent, dosage is 2.15g/kg；
Blank group: the normal saline of gavage equivalent.
Above-mentioned group mice daily three times, takes after brewing by warm water.It is administered one every day by above-mentioned dose of capacity
Secondary, successive administration eight days, 60min after being administered with last, again measure the asthmatic latent period of every Cavia porcellus and tic number of animals (by
Dyspnea causes the number of animals fallen of suffocating, in 6min), the results are shown in Table 3.
The test of relievining asthma of table 3 enzyme composition gavage Cavia porcellus
As can be known from Table 3, after basic, normal, high three dosage enzyme compositions gavage eight days to Cavia porcellus, before self administration
Rear contrast, all can be obviously prolonged asthmatic latent period；And can substantially reduce because dyspnea causes the number of animals suffocated and fall, with
Blank group ratio, middle high dose enzyme composition also has phase same-action, shows that enzyme composition has antiasthmatic effect, cut along with agent
Amount increases, and antiasthmatic effect increases, with positive control drug ratio, under identical clinical multiple dosage, antiasthmatic effect and compound recipe cough with asthma glue
Wafer is suitable.
(2) antitussive action test: select Cavia porcellus citric acid to draw the method for coughing
1, preliminary election animal: take body weight 180～210g Cavia porcellus, male and female dual-purpose, be placed in the closed glass cover of 4L volume,
The pressure of 80kpa (600mmHg), sprays into 17.5% citric acid by nebulization (being sprayed into by glass shower nozzle), and spray 1min, record
In 5min, the cough number of times of Cavia porcellus, carries out preliminary election, and in 5min, cough number of times is less than 10 persons, and reject need not.
2, formal test: take the qualified Cavia porcellus of preliminary election 50, is randomly divided into five groups, often group 10.
It is divided into blank, positive control drug and basic, normal, high three dose of test medicine groups.
Packet situation is:
Low dose group: the enzyme composition of gavage embodiment 3, dosage is 2g/kg；
Middle dosage group: the enzyme composition of gavage embodiment 3, dosage is 4g/kg；
High dose group: the enzyme composition of gavage embodiment 3, dosage is 6g/kg；
Positive controls: gavage compound recipe medical capsules for treating asthma agent, dosage is 2.15g/kg；
Blank group: the normal saline of gavage equivalent.
It is administered once a day by above-mentioned dose of capacity, continuous eight days, 60min after last is administered, then by condition during preliminary election,
Giving to cough in drawing, record cough number of times in the cough latent period of every Cavia porcellus and 5min, it the results are shown in Table 4.
The cough-relieving test of table 4 enzyme composition gavage Cavia porcellus
As can be known from Table 4, basic, normal, high three kinds of various dose enzyme compositions gavage to Cavia porcellus, with blank group ratio
Relatively, all having obvious antitussive action, make cough latent period be obviously prolonged, cough number of times significantly reduces, and middle high dose ferment combines
Thing, its antitussive action is better than compound recipe medical capsules for treating asthma agent, and low dosage enzyme composition, with positive control drug ratio without significant difference.
(3) phlegm-dispelling functions test: use mice phenol red secretion test.
Taking body weight 20～24g healthy mice 50, be randomly divided into five groups, often group 10, basic, normal, high Three doses is subject to
Reagent thing group, positive control drug and blank group.
Packet situation is:
Low dose group: the enzyme composition of gavage embodiment 3, dosage is 2g/kg；
Middle dosage group: the enzyme composition of gavage embodiment 3, dosage is 4g/kg；
High dose group: the enzyme composition of gavage embodiment 3, dosage is 6g/kg；
Positive controls: gavage compound recipe medical capsules for treating asthma agent, dosage is 2.15g/kg；
Blank group: the normal saline of gavage equivalent.
It is administered once a day by above-mentioned metering, continuous eight days.With 30min after last gastric infusion, by lumbar injection
Phenol red solution 0.5ml (0.25g/dl) injection half an hour after takes off cervical vertebra and puts to death mice, faces upward position and is fixed on surgical plate, cuts off through just
Middle skin, separates trachea, and No. 7 syringe needles polished by head under larynx insert tracheal strips about 0.3cml, after fixing with silk thread ligation,
Draw 5%NaHCO3 solution 0.5ml with 1ml syringe, by syringe needle lavation respiratory tract 3 times back and forth, irrigating solution is injected a ratio
In colour tube, again draw NaHCO3 solution 0.5ml, as above lavation three times；The same lavation of third time again, by the washing liquid of three times
(about 1.5ml) puts in color comparison tube, with spectrophotometer densitometric at 546nm wavelength, with 5%NaHCO3 liquid as blank, sees
Table 5.
The expectorant test of table 5 enzyme composition gavage Cavia porcellus
As can be known from Table 5, basic, normal, high Three doses enzyme composition and compound recipe cough curing capsule agent and blank
Group compares, and its phenol red eliminating amount dramatically increases, and shows there is certain phlegm-dispelling functions, and no significant difference compared by two medicines.
Model case
Case 1: Chen, female, 31 years old, continue because catching a cold to heal more than the moon, feel uncomfortable in chest the most afterwards, discomfort in the throat, failed at that time to adjust
Controlling, asthma occurs then, failing to lie flat due to dyspnea time serious, cough is spat few, is bronchial asthma after diagnosing.Take ferment of the present invention
After promotor composition 3 days, disease amelioration, outbreak alleviates, decreased duration during outbreak, and uncomfortable in chest, singultus removes, continues to take 20
My god, transference cure, recover.
Case 2: Lee, man, 23 years old, belong to intermission asthma and continue patient, every year morbidity 23 times, the most in the winter time,
With acutely cough, by taking ferment effervescent granule of the present invention, after medication two weeks, recovery from illness there is no recurrence.
Case 3: Zhang, man, 36 years old, because flu suffers from tracheitis, asthma, the state of an illness was heavier, takes relieving cough and asthma of the present invention
Ferment effervescent granule within 5 days, take effect, after 1 month, the state of an illness substantially alleviates, and continues to take the ferment effervescent granule 2 months of the present invention
The rear state of an illness is fully recovered, and does not recurs so far.
Claims (10)
1. an enzyme composition with cough and asthma relieving efficacy, it is characterised in that this enzyme composition includes following component: benefit
Raw bacterium 0.1-15 weight portion, plant enzyme stock solution 10-120 weight portion and Chinese medicine extract 10-80 weight portion；Described ferment combines
Thing is to mix with plant enzyme stock solution after the sterilized technique of Chinese medicine extract, and inoculates beneficial flora and carry out sealed fermenting and form；
Described plant enzyme stock solution is fermented in the hermetic container that temperature is 30-40 DEG C by after fruit and vegerable impregnation liquid inoculation beer yeast
2-5d and obtain；Wherein beer yeast inoculum concentration is the 7-10% of fruit and vegerable stock solution weight；
Described Chinese medicine extract is extracted by following bulk drugs and forms: Herba Portulacae 2-10 weight portion, Herba Ardisiae Japonicae 2-10 weight portion, Herba Asari
1-8 weight portion, Fructus Momordicae 2-10 weight portion, Radix Rhodiolae 2-8 weight portion, Fructus Citri tangerinae core 1-8 weight portion, slurry grass 0.5-6 weight portion, thin
Lotus 0.5-6 weight portion, Caulis Mahoniae 0.5-6 weight portion and Pericarpium Citri Reticulatae 0.5-5 weight portion.
There is the enzyme composition of cough and asthma relieving efficacy the most as claimed in claim 1, it is characterised in that described fruit and vegerable impregnation liquid by
Fruit and vegetable materials, white sugar and pure water mixing are made through ozone sterilization after standing 24h, the weight of described fruit and vegetable materials, white sugar and water
Portion rate is 3-4:1:6, and described fruit and vegetable materials is pears, Folium Eriobotryae and Fructus Pruni.
There is the enzyme composition of cough and asthma relieving efficacy the most as claimed in claim 1, it is characterised in that described probiotic bacteria is prebiotic
Bacteria powder, is uniformly mixed by following at least two powder:
Bacillus bifidus 1.5-3 weight portion, lactobacillus rhamnosus 2-2.5 weight portion, lactobacillus casei 1.2-2 weight portion, addicted to yogurt
Bacillus 1.4-2.5 weight portion and Lactobacillus plantarum 2-3 weight portion, the viable bacteria content of described probiotic bacteria powder is 1 × 108-5×
108cfu/g。
There is the enzyme composition of cough and asthma relieving efficacy the most as claimed in claim 1, it is characterised in that described enzyme composition is also
Including stachyose 10-50 weight portion and vitamin B group's 12-35 weight portion.
5. enzyme composition as claimed in claim 4, it is characterised in that described enzyme composition includes following component: prebiotic
Bacterium 12.5 weight portion, plant enzyme stock solution 86 weight portion, Chinese medicine extract 40.7 weight portion, stachyose 26 weight portion and dimension are raw
Element B group 22.5 weight portion；Wherein, described Chinese medicine extract is extracted by following crude drug and forms:
Herba Portulacae 5.8 weight portion, Herba Ardisiae Japonicae 4.5 weight portion, Herba Asari 5 weight portion, Fructus Momordicae 4.5 weight portion, Radix Rhodiolae 3.2 weight
Part, Fructus Citri tangerinae core 4 weight portion, starch careless 3.5 weight portions, Herba Menthae 3.5 weight portion, Caulis Mahoniae 3.2 weight portion and Pericarpium Citri Reticulatae 2 weight portion.
6. an effervescent granule with cough and asthma relieving efficacy, it is characterised in that described effervescent granule is by claim 1 to 5
One of them described enzyme composition and adjuvant are made, and the ratio of weight and number of described compositions and adjuvant is 1:0.5-1:3.
7. effervescent granule as claimed in claim 5, it is characterised in that described adjuvant include pregelatinized starch 35-80 weight portion,
Polyethylene glycol 6000 15-40 weight portion, sodium bicarbonate 60-132 weight portion, tartaric acid 35-80 weight portion, D-xylose 10-40 weight
Amount part, Thomas balsam 30-60 weight portion and methylcellulose 16-52 weight portion.
8. effervescent granule as claimed in claim 7, it is characterised in that described effervescent granule is by following enzyme composition and adjuvant
Processed it is prepared from:
Enzyme composition: 187.7 weight portions；
Adjuvant: pregelatinized starch 64 weight portion, polyethylene glycol 6000 23 weight portion, sodium bicarbonate 118 weight portion, tartaric acid 45
Weight portion, D-xylose 28 weight portion, Thomas balsam 51 weight portion and methylcellulose 34 weight portion.
9. one kind by the preparation method of the enzyme composition described in claim 4 with cough and asthma relieving efficacy, it is characterised in that institute
The method of stating comprises the steps:
(1) preparation of Chinese medicine extract:
(1.1) take the Herba Asari of described weight portion, Herba Ardisiae Japonicae, Fructus Citri tangerinae core and slurry grass and carry out soak by water 3 times, 15 times amount decocting for the first time
Boiling 2-4h, for the second time 12 times amount soak by water 1-1.5h, for the third time 10 times amount soak by water 1-1.5h, merging filtrate, temperature is 45 DEG C
Under the conditions of to be concentrated into relative density be 1.00-1.15g/ml, cooling, add 50-60% ethanol, stand overnight, filter, filtrate is in temperature
Degree is concentrated into, under the conditions of 50-60 DEG C, the thick paste that relative density is 1.00-1.25g/ml, adds water to total amount 50-70%, stands
30-40h, takes supernatant, obtains solution 1；
(1.2) Herba Portulacae of recipe quantity, Fructus Momordicae, Radix Rhodiolae, Herba Menthae, Caulis Mahoniae and Pericarpium Citri Reticulatae are weighed, boiling twice, every time
1h；Amount of water is add raw material gross weight 10 times for the first time；Amount of water is add raw material gross weight 8 times for the second time；Merge and decoct
Liquid, filters, obtains solution 2；
(1.3) solution 2 mix homogeneously that the solution 1 step (1.1) prepared and step (1.2) prepare, obtains Chinese medicine extract；
(2) preparation of plant enzyme stock solution: added to by white sugar in fruit and vegetable materials, is simultaneously introduced pure water mix homogeneously, stands
After extraction 24h, carry out ozone sterilization, filter, obtain planting dip；It is fruit and vegerable stock solution weight by fruit and vegerable impregnation liquid inoculation inoculum concentration
8% beer yeast, in the hermetic container that temperature is 30-40 DEG C ferment 3d, obtain plant enzyme stock solution；(3) Chinese medicine is carried
Take thing and the mixing of plant enzyme stock solution, add probiotic bacteria, stachyose and vitamin B group, be the hermetic container of 30-40 DEG C in temperature
Middle fermentation 5-7d, filters, and collects filtrate, and filtrate carries out low temperature spray drying, obtains the enzyme composition of powdery.
10. one kind by the preparation method of effervescent granule described in claim 8, it is characterised in that described method comprises the steps:
A. prepare the enzyme composition of powdery, weigh formula ratio and cross 60 mesh sieves, stand-by；
B. baking oven put into by the pregelatinized starch and the D-xylose that weigh formula ratio, dries 3h under the conditions of 60 DEG C, crosses 60 mesh after cooling
Sieve, stand-by；
C. weigh the sodium bicarbonate of formula ratio, dissolve with the ethanol solution of 60-80%, add the ferment that step a of 1/2 weight processes
The pregelatinized starch of the step b process of promotor composition and 1/2 weight and D-xylose, make soft material, wet granulation, and low temperature is done
Dry, granulate, obtain bicarbonate soda granule；
D. weigh the tartaric acid polyethylene glycol 6000 embedding after melting of formula ratio, cooling crush, cross 60 mesh sieves；
E. the tartaric acid after step d being processed is mixed homogeneously with Thomas balsam, dissolves with the ethanol solution of 60-80%, adds 1/2
Enzyme composition that step a of weight processes and the pregelatinized starch that step b of 1/2 weight processes and D-xylose, make soft
Material, wet granulation, it is dried, granulate, obtains granulates；
Granulates mixing prepared by the bicarbonate soda granule f. step c prepared and step d, adds methylcellulose and uniformly mixes
Close, cross 80 mesh sieves, pelletize, cold drying, arrange, prepare effervescent granule.
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| CN108029786A (en) * | 2017-12-12 | 2018-05-15 | 滁州尹氏油脂有限公司 | A kind of relieving cough and reducing sputum hawthorn sesame oil |
| CN108244434A (en) * | 2018-02-01 | 2018-07-06 | 南昌大学 | A kind of fermentation loquat pear beverage with relieving cough and moistening lung and preparation method thereof |
| CN109247459A (en) * | 2017-07-14 | 2019-01-22 | 程艳青 | A kind of pyrus nivalis root of kirilow rhodiola relieving cough and moistening lung health functional beverage and processing method |
| CN109770347A (en) * | 2019-01-09 | 2019-05-21 | 广东微量元素生物科技有限公司 | A kind of relieving cough and reducing sputum dandelion, pyrus nivalis crystallite medicinal granules of powder and preparation method thereof |
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| CN102669658A (en) * | 2012-04-23 | 2012-09-19 | 浙江科技学院 | Preparation method of plant enzyme food |
| CN103844266A (en) * | 2014-03-25 | 2014-06-11 | 容瑜 | Natural conditioning and nourishing ferment and manufacturing method thereof |
| CN105639394A (en) * | 2015-12-29 | 2016-06-08 | 湖北老乡农业科技有限公司 | Cyclocarya paliurus leaf enzyme effervescent tablets and preparation method thereof |
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| CN102669658A (en) * | 2012-04-23 | 2012-09-19 | 浙江科技学院 | Preparation method of plant enzyme food |
| CN103844266A (en) * | 2014-03-25 | 2014-06-11 | 容瑜 | Natural conditioning and nourishing ferment and manufacturing method thereof |
| CN105639394A (en) * | 2015-12-29 | 2016-06-08 | 湖北老乡农业科技有限公司 | Cyclocarya paliurus leaf enzyme effervescent tablets and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109247459A (en) * | 2017-07-14 | 2019-01-22 | 程艳青 | A kind of pyrus nivalis root of kirilow rhodiola relieving cough and moistening lung health functional beverage and processing method |
| CN108029786A (en) * | 2017-12-12 | 2018-05-15 | 滁州尹氏油脂有限公司 | A kind of relieving cough and reducing sputum hawthorn sesame oil |
| CN108244434A (en) * | 2018-02-01 | 2018-07-06 | 南昌大学 | A kind of fermentation loquat pear beverage with relieving cough and moistening lung and preparation method thereof |
| CN109770347A (en) * | 2019-01-09 | 2019-05-21 | 广东微量元素生物科技有限公司 | A kind of relieving cough and reducing sputum dandelion, pyrus nivalis crystallite medicinal granules of powder and preparation method thereof |
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Application publication date: 20161207 |