CN108244434A - A kind of fermentation loquat pear beverage with relieving cough and moistening lung and preparation method thereof - Google Patents

A kind of fermentation loquat pear beverage with relieving cough and moistening lung and preparation method thereof Download PDF

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Publication number
CN108244434A
CN108244434A CN201810101510.6A CN201810101510A CN108244434A CN 108244434 A CN108244434 A CN 108244434A CN 201810101510 A CN201810101510 A CN 201810101510A CN 108244434 A CN108244434 A CN 108244434A
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loquat
fermentation
lactobacillus
beverage
pear beverage
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谢明勇
熊涛
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Nanchang University
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Nanchang University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/34635Antibiotics
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/3481Organic compounds containing oxygen
    • A23L3/3508Organic compounds containing oxygen containing carboxyl groups
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The present invention provides a kind of sweet and sour taste, nutrient and flavour is all good, long shelf-life and without essence, pigment, preservative fermentation loquat pear beverage.The characteristics of loquat pear beverage prepared by the present invention, is:(1) using three plants of bacterium composite fermentations, fermentation time reduction to 6~10h;(2) any preservative need not be added in beverage can preserve for a long time, and long shelf-life was up to 1 year or more;(2) dietary fiber, vitamin and other nutritional ingredients being effectively retained in loquat and pears, while be also mused together original flavor of two kinds of fruit of loquat and pears well;(3) essence and other any additives for adjusting flavor need not be added in beverage.(4) fermentation loquat pear beverage prepared by the present invention, relative to unfermentable loquat pear beverage relieving cough and moistening lung effect more preferably;(5) present invention process is simple, easily controllable, and standardization level is high, it is easy to realize large-scale industrial production.

Description

A kind of fermentation loquat pear beverage with relieving cough and moistening lung and preparation method thereof
Technical field
The present invention relates to a kind of vegetalitas lactacidase fermenting beverages and preparation method thereof, and in particular to kind has relieving cough and moistening lung Fermentation loquat pear beverage of effect and preparation method thereof.
Background technology
Pears are one of main temperate zone fruit in China, and variety source is very abundant.The crisp succulence of its meat, sweet tea acid is tasty, moisture Abundance is called " autumn-dryness disease jinx ", in pear flesh containing abundant sugar, moisture and malic acid, citric acid, carrotene, calcium, The nutriments such as phosphorus, iron.The refrigerant sweetness of pears nature and flavor is thought in traditional medicine, enters lung, have promote the production of body fluid, moisturize, heat-clearing, resolving sputum work( Effect.
Loquat is also important nutrition and health care fruit, has cough-relieving, moistening lung, diuresis, stomach invigorating, heat-clearing cooling and other effects, Ming Dynasty's physician's Li Shizhen (1518-1593 A.D.) early in《Compendium of Materia Medica》In the effect of just having affirmed loquat:" loquat, cough-relieving are coughed, and quench the thirst lower gas, sharp lung Gas, antiemetic is inverse, Your Majesty Jiao Re, adjusts the five internal organs ".Leaf, the core of loquat can be used as medicine, the traditional Chinese medical science commonly use with the lung that disappears, cough-relieving, wet one's whistle, Xie Wen, And stomach.Loguat leaf bitter, mild-natured, wherein the amygdalin and saponin that contain, amygdalin has an antitussive effect, saponin have eliminating the phlegm and Inhibit the effect of influenza virus.
Since pears and loquat all have moistening lung, cough-relieving and other effects, in life, people often endure pears and loquat together Soup has good food therapy effect, but the shelf-life is too short, and carries, transport all inconveniences, so can only make at home, no Easily realize large-scale industrial production.Also have currently on the market and loquat and pears are processed into various beverage products, but processing side Formula is typically all to collect fruit juice by squeezing the juice, and is then filtered processing to fruit juice again, a large amount of meals are lost in this process The nutriments such as fiber are eaten, so the nutritive value of beverage products is had a greatly reduced quality.In addition, in order to improve mouthfeel and color and luster and prolong The long shelf-life, the additives such as a large amount of essence, pigment, preservative are often added in product, long-term consumption is also unfavorable for human body Health.
Invention content
In view of the above-mentioned problems, the problem to be solved in the present invention is to provide, a kind of sweet and sour taste, nutrient and flavour be all good, the shelf-life Fermentation loquat pear beverage that is long and being free of essence, pigment, preservative, production method are suitable for the large-scale standard metaplasia of factory Production.
The present invention is achieved by the following technical solutions:
A kind of preparation method of loquat pear beverage with relieving cough and moistening lung, step are as follows:
It chooses without rotten, ripe, fresh loquat and pyrus nivalis, after rinsing well, loquat and pyrus nivalis is enucleated, go to obstruct, gone Be positioned over after skin it is spare in pure water, mashing, by loquat slurry 15-20%, pyrus nivalis slurry 15-20%, glucose 0.01%~10%, Sodium isoascorbate 1-2 ‰, pure water 49.8%~69.89% mass ratio stir evenly, sterilize, sterilising temp be 85~110 DEG C, sterilization time is 2 seconds~10 minutes;For the postcooling that sterilizes to 20~45 DEG C, inoculation contains lactobacillus plantarum, secondary cheese breast bar Three kinds of bacterium, lactobacillus acidophilus compound lactobacillus agent with excellent fermenting property, inoculum concentration 103~106Cfu/ml, 25~45 DEG C fermentation 6~10 hours, pH value 3.5~4.5 terminate fermentation.By the canned sealing of loquat pear beverage after fermentation, then 90 DEG C sterilizing 30 minutes to get finished product.
The number of viable ratio of three kinds of bacterium is 1 in the compound lactobacillus agent:1:1;
Lactobacillus plantarum of the present invention be the autonomous selection and breeding in laboratory one plant of good plant lactobacillus NCU116, in On December 16th, 2015 is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (address:Court of Beijing The positive institute 3 of area's North Star West Road 1, Institute of Microorganism, Academia Sinica), CGMCC is referred to as, deposit number is CGMCC11885;
Lactobacillus paracasei of the present invention is the excellent secondary Lactobacillus paracasei of one plant of the autonomous selection and breeding in laboratory NCU622 is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (on December 16th, 2015 Location:Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica), CGMCC is referred to as, preservation is compiled Number be CGMCC11886;
Lactobacillus acidophilus of the present invention is one plant of lactobacillus acidophilus NCU402 of the autonomous selection and breeding in laboratory, in 2015 On December 16, in is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (address:Chaoyang District, Beijing City The institute 3 of North Star West Road 1, Institute of Microorganism, Academia Sinica), it is referred to as CGMCC, deposit number CGMCC11887.
The characteristics of loquat pear beverage prepared by the present invention, is:(1) using three plants of bacterium composite fermentations, fermentation time reduction It to 6~10h, reduces by more than half, fermentation efficiency greatly improves;(2) by lactobacillus-fermented, a large amount of lactic acid and lactic acid are generated The natural bacteriostatics substance such as streptostacin, so any preservative need not be added in beverage to be preserved for a long time, long shelf-life is up to one Year or more;(2) it is beaten by full fruit, dietary fiber, vitamin and other nutritional ingredients being effectively retained in loquat and pears, together When also original flavor of two kinds of fruit of loquat and pears is mused together well;(3) pass through compound lactobacillus-fermencucumber, production Given birth to natural soft tart flavour, imparted the unique ferment local-flavor of loquat pear beverage, thus need not be added in beverage essence and its The additive of his any adjusting flavor.(4) fermentation loquat pear beverage prepared by the present invention, relative to unfermentable loquat pear beverage Relieving cough and moistening lung effect is more preferably;(5) present invention process is simple, easily controllable, and standardization level is high, it is easy to realize large-scale industry Metaplasia is produced.
Specific embodiment
Example below is only to further illustrate the present invention, range that the invention is not limited in any way.
Embodiment 1. is chosen without rotten, ripe, fresh loquat and pyrus nivalis, after rinsing well, loquat and pyrus nivalis are enucleated, Go to obstruct, remove the peel after be positioned over spare in pure water, mashing, by loquat slurry 15%, pyrus nivalis slurry 20%, glucose 10%, sodium isoascorbate 1 ‰, the ratio of pure water 54.9% stirs evenly, sterilizing, and sterilising temp is 102 DEG C, and sterilization time is 10 minutes;It is cold after sterilizing But to 37 DEG C, be inoculated with has the compound of excellent fermenting property containing lactobacillus plantarum, Lactobacillus paracasei, three kinds of lactobacillus acidophilus Agent of lactic acid bacteria, inoculum concentration 106Cfu/ml, 37 DEG C ferment 6 hours, and pH value 3.5 terminates fermentation.By loquat pears after fermentation Then the canned sealing of beverage sterilizes 30 minutes to get finished product for 90 DEG C.
Embodiment 2. is chosen without rotten, ripe, fresh loquat and pyrus nivalis, after rinsing well, loquat and pyrus nivalis are enucleated, Go to obstruct, remove the peel after be positioned over spare in pure water, mashing, by loquat slurry 20%, pyrus nivalis slurry 20%, glucose 10%, sodium isoascorbate 1 ‰, the ratio of pure water 49.9% stirs evenly, sterilizing, and sterilising temp is 102 DEG C, and sterilization time is 10 minutes;It is cold after sterilizing But to 37 DEG C, be inoculated with has the compound of excellent fermenting property containing lactobacillus plantarum, Lactobacillus paracasei, three kinds of lactobacillus acidophilus Agent of lactic acid bacteria, inoculum concentration 104Cfu/ml, 37 DEG C ferment 10 hours, and pH value 3.8 terminates fermentation.By loquat after fermentation Then the canned sealing of pear beverage sterilizes 30 minutes to get finished product for 90 DEG C.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, therefore, all people in the art The design of member according to the present invention passes through the available skill of logical analysis, reasoning, or a limited experiment in prior art basis Art scheme, all because determined by the claims in protection domain.
Experiment one:Zoopery
1. materials and methods:
1.1 experiment materials and animal
Experiment material:The fermentation loquat pear beverage obtained of embodiment 1.
Experimental animal:Kunming mice, weight 18-22g are provided by medical college of University Of Nanchang Experimental Animal Center.
1.2 experimental method
1.2.1 mouse draws cough experiment
25% concentrated ammonia liquor metered dose is carried out using ultrasonic atomizatio sprayer, is taken out immediately after 10 seconds, record mouse is from connecing Started by spraying to the cough number occurred in the incubation period coughed and 2 minutes, 2 minutes not cough persons give up after spraying.It closes Lattice mouse is randomly divided into four groups, respectively blank group, loquat pear beverage group of not fermenting, fermentation after 2 days according to above-mentioned group technology Loquat pear beverage group (embodiment 1), negative control group, wherein blank group select grouping at random before cough experiment is drawn, without dense Ammonia spraying.
1.2.2 cough-relieving is tested
Every group daily by the dosage gavage 2 times of 10mL/kg respectively, continuous gavage 5 days, blank group is given with negative control group Normal saline.In 1 hour after the last administration, receive 25% concentrated ammonia liquor metered dose respectively, taken out immediately after 10 seconds.Record Since mouse to the cough number occurred in the incubation period coughed and 2 minutes receiving to spray, and cough person is had no in 2 minutes, and it is latent The volt phase was remembered with 120 seconds, the results are shown in Table 1.
1 cough-relieving experiment effect of table compares
Group N/ is only Dosage (g/Kg) Cough latent period/second Cough/number in 120 seconds Cough inhibiting rate/%
Blank group 30 10 120 0 -
Negative control group 30 10 9.3±6.58 30.3±29.5 -
Do not ferment loquat pear beverage 30 10 67.3±42.1** 2.9±2.68** 86.9
Ferment loquat pear beverage 30 10 98.5±39.57** 0.6±1.07** 97.6
Note:Compared with negative control group, * * P≤0.01
Experiment two:Crowd tests
200 adults for having cough symptom are chosen, are randomly divided into 4 groups, respectively blank group, 1 group of embodiment, embodiment 2 groups, loquat pear beverage group of not fermenting, every group of 50 people are not treated for each person every day by drug, take respectively physiological saline, Fermentation loquat pear beverage, the fermentation loquat pear beverage of 2 method of embodiment preparation and unfermentable loquat pears drink prepared by 1 method of embodiment Material, 3 times for each person every day, a 250mL, after continuously taking 7 days, four groups of effective percentage is respectively 40%, 94%, 96%, 72%.
Two groups of experiments confirm that fermentation loquat pear beverage prepared by the present invention has the effect that moistens the lung and relieve the cough well.

Claims (3)

1. a kind of preparation method of loquat pear beverage with relieving cough and moistening lung, it is characterised in that:Step is as follows:It chooses without corruption Rotten, ripe, fresh loquat and pyrus nivalis, after rinsing well, by loquat and pyrus nivalis stoning, go to obstruct, remove the peel after be positioned over pure water In spare, mashing, by loquat slurry 15-20%, pyrus nivalis slurry 15-20%, glucose 0.01%~10%, sodium isoascorbate 1-2 ‰, pure The mass ratio of water purification 49.8%~69.89% stirs evenly, sterilizing, and sterilising temp is 85~110 DEG C, and sterilization time is 2 seconds ~10 minutes;For the postcooling that sterilizes to 20~45 DEG C, inoculation contains lactobacillus plantarum, Lactobacillus paracasei, three kinds of lactobacillus acidophilus Compound lactobacillus agent with excellent fermenting property, inoculum concentration 103~106Cfu/ml, 25~45 DEG C ferment 6~10 hours, pH Value 3.5~4.5 terminates fermentation;By the canned sealing of loquat pear beverage after fermentation, then 90 DEG C of sterilizings 30 minutes to get into Product.
2. a kind of preparation method of loquat pear beverage with relieving cough and moistening lung as described in claim 1, it is characterised in that: Step is as follows:It chooses without rotten, ripe, fresh loquat and pyrus nivalis, after rinsing well, loquat and pyrus nivalis is enucleated, go to obstruct, gone Spare in pure water, mashing is positioned over after skin, by loquat slurry 15%, pyrus nivalis slurry 20%, glucose 10%, sodium isoascorbate 1 ‰, pure The ratio of water purification 54.9% stirs evenly, sterilizing, and sterilising temp is 102 DEG C, and sterilization time is 10 minutes;The postcooling that sterilizes is to 37 DEG C, it is inoculated with containing three kinds of lactobacillus plantarum, Lactobacillus paracasei, lactobacillus acidophilus compound lactobacillus with excellent fermenting property Agent, inoculum concentration 106Cfu/ml, 37 DEG C ferment 6 hours, and pH value 3.5 terminates fermentation;By loquat pear beverage tank after fermentation Then dress sealing sterilizes 30 minutes to get finished product for 90 DEG C.
3. a kind of preparation method of loquat pear beverage with relieving cough and moistening lung as described in claim 1, it is characterised in that: Step is as follows:It chooses without rotten, ripe, fresh loquat and pyrus nivalis, after rinsing well, loquat and pyrus nivalis is enucleated, go to obstruct, gone Spare in pure water, mashing is positioned over after skin, by loquat slurry 20%, pyrus nivalis slurry 20%, glucose 10%, sodium isoascorbate 1 ‰, pure The ratio of water purification 49.9% stirs evenly, sterilizing, and sterilising temp is 102 DEG C, and sterilization time is 10 minutes;The postcooling that sterilizes is to 37 DEG C, it is inoculated with containing three kinds of lactobacillus plantarum, Lactobacillus paracasei, lactobacillus acidophilus compound lactobacillus with excellent fermenting property Agent, inoculum concentration 104Cfu/ml, 37 DEG C ferment 10 hours, and pH value 3.8 terminates fermentation;By loquat pear beverage after fermentation Then canned sealing sterilizes 30 minutes to get finished product for 90 DEG C.
CN201810101510.6A 2018-02-01 2018-02-01 A kind of fermentation loquat pear beverage with relieving cough and moistening lung and preparation method thereof Pending CN108244434A (en)

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Cited By (4)

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CN109832618A (en) * 2019-01-04 2019-06-04 中国食品发酵工业研究院有限公司 A kind of lily fermented product and preparation method thereof
CN111134257A (en) * 2020-01-17 2020-05-12 河北科技大学 Lactic acid bacteria fermented Chinese herbal medicine pear juice and preparation method thereof
CN111887364A (en) * 2020-08-11 2020-11-06 义乌市沁润食品有限公司 Plant fresh fruit beverage and preparation method thereof
CN115812953A (en) * 2022-12-22 2023-03-21 中国农业科学院果树研究所 Cough relieving soft extract and preparation method thereof

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Publication number Priority date Publication date Assignee Title
CN109832618A (en) * 2019-01-04 2019-06-04 中国食品发酵工业研究院有限公司 A kind of lily fermented product and preparation method thereof
CN111134257A (en) * 2020-01-17 2020-05-12 河北科技大学 Lactic acid bacteria fermented Chinese herbal medicine pear juice and preparation method thereof
CN111887364A (en) * 2020-08-11 2020-11-06 义乌市沁润食品有限公司 Plant fresh fruit beverage and preparation method thereof
CN115812953A (en) * 2022-12-22 2023-03-21 中国农业科学院果树研究所 Cough relieving soft extract and preparation method thereof

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