CN106974279A - Prepare the fermentation composition and preparation method for being applied to cardiovascular and cerebrovascular diseases conditioning and its routine servicing effect plant enzyme - Google Patents
Prepare the fermentation composition and preparation method for being applied to cardiovascular and cerebrovascular diseases conditioning and its routine servicing effect plant enzyme Download PDFInfo
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Landscapes
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Abstract
The invention provides the fermentation composition for preparing the plant enzyme for being applied to cardiovascular and cerebrovascular diseases conditioning and its routine servicing, mainly it is made up of 0.01~10 part of 3~22 parts of functional oligose, 0.1~5 part of probiotics and Chinese medical extract;Chinese medical extract is extracted by the raw material of following parts by weight and formed:0.3~2 part of matrimony vine, 0.5~3 part of mulberries, 0.1~1.6 part of sea-buckthorn, 0.5~3 part of the root of kudzu vine, 0.5~2 part of cassia seed, 0.1~0.8 part of aloe and 0.3~2 part of hawthorn.The fermentation composition is novel fermentation product, is that the bacterium bag is easy to use for preparing ferment " fermentation bacterium bag ", the industrialized production of suitable all kinds of plant enzymes.
Description
Technical field
The invention belongs to prevent conditioning maintenance product field, more particularly to one kind prepare suitable for cardiovascular and cerebrovascular diseases conditioning and its
The fermentation composition and preparation method of routine servicing effect plant enzyme.
Background technology
Plant enzyme is always the best seller of ferment class product.Plant enzyme is by tens of kinds of different vegetables and fruits, cereal, seas
The plant such as algae and mushroom class, develops through spontaneous fermentation, remains the nutrition elite in plant, contains the various of human body needs
Ferment, oligosaccharides and a variety of vitamins and mineral matter, can produce abundant SOD antioxidase compositions again, improve anti-oxidant energy in vivo
Power, and then strengthen immunity.
Existing traditional handicraft and modern crafts to ferment has all done further improvement, while also occurring in that some addition benefits
Health food prepared by the ferment and ferment of raw bacterium.Probiotics fermention fruits and vegetables, can activate the effective efficiency composition in fruits and vegetables, molten
Go out active effect material of more elite.The heart, cerebrovascular metabolic function plant ferment can be improved as disclosed in patent CN103355666A
In the preparation method of plain drink, using various plants as Solution culture method base, using compound probiotic as conversion instrument, sent out using liquid
Drink prepared by ferment technique, record can improve body metabolism disorder, and prevention of cardiovascular disease reduces " three high ".But the patent
Formula components it is complicated, and can not be on a large scale flexibly using or being used in the industrial manufacturing processes of different ferment;In order to carry
Effect of the high ferment in terms of metabolism, coordinating intestines and stomach function in vivo is promoted, and adapt to use on a large scale, applicant
The fermentate for preparing ferment is further improved, and develops the formula for the function affect for improving ferment.
There is physiologically active, not by human body hydrochloric acid in gastric juice, gastric enzyme degraded in addition, functional oligose is a class, do not inhaled in small intestine
Receive, reach the oligosaccharide of big intestines;Oligosaccharide is prebiotics, is the substrate of the growth of probiotics, and with promoting, human body is prebiotic
The physiological functions such as the propagation of bacterium.By the way that probiotics and functional oligose are used in conjunction with, between the two with synergy, energy
Promote the regulatory function to gut flora.Co between probiotics and oligosaccharide, be probiotic in symphysis unit and
Co between prebiotics, they complement each other, and play a role jointly, promote beneficial bacterium growing multiplication, suppress harmful bacteria
Growth;Research shows that both uses can increase the quantity of Bifidobacterium in healthy human faecal mass, but individually take probiotics and have no
This effect.
Based on above-mentioned technology, the artificial feature for improving ferment of application is added with the ferment fermentate of prior art
The traditional Chinese medicine ingredients of effect, enable its ferment prepared effectively to adjust gut flora, strengthen immunity, and have function.
On the other hand, existing ferment bucket, prepares plant enzyme so that plant enzyme using fruit, sugar and water as raw material
It can be prepared in family, it is easy to operate.But also having many weak points, the time prepared by 1. ferment is still longer, Er Qierong
Easily prepare failure;2. made from plant enzyme effect it is indefinite, user can not oneself control prepare effect of plant enzyme, than
Such as there is the plant enzyme of conditioning cardiovascular and cerebrovascular effect;3. the low molecular sugar and polysaccharide majority in the plants such as fruit can not be utilized,
The oligosaccharide of given efficacy can not be converted into.For the deficiency, applicant, which puts forth effort research, to be had shortening ferment preparation time, makes
With it is convenient and nurse one's health suitable for cardiovascular and cerebrovascular diseases and routine servicing effect fermentation composition, i.e. ferment bag.
The content of the invention
It is contemplated that at least solving one of technical problem present in prior art.Therefore, one object of the present invention
It is to propose a kind of fermentation composition for preparing the plant enzyme suitable for cardiovascular and cerebrovascular diseases conditioning and its routine servicing, the fermentation
Composition is directly putting type fermented microbial inoculum, and probiotics and functional oligose are used in combination, while adding traditional Chinese medicine ingredients, make the group
Compound can promote the conversion of small molecular sugar and polysaccharide to oligosaccharide while with good gut flora regulatory function.
Plant enzyme prepared by the fermentation composition may be adapted to cardiovascular and cerebrovascular diseases conditioning, have day to the crowd with cardio-cerebrovascular disease
The effect often conserved.
Technical scheme is as follows:
A kind of fermentation composition for preparing the plant enzyme suitable for cardiovascular and cerebrovascular diseases conditioning and its routine servicing, the hair
Ferment composition is direct putting type plant enzyme fermenting agent, and the fermentation composition includes the component of following parts by weight:Feature is low
0.01~10 part of 3~22 parts of glycan, 0.1~5 part of probiotics and Chinese medical extract,
Wherein, the probiotics is the one or more in Bifidobacterium, lactobacillus and streptococcus thermophilus;
The Chinese medical extract is extracted by the raw material of following parts by weight and formed:0.3~2 part of matrimony vine, 0.5~3 part of mulberry
Shen, 0.1~1.6 part of sea-buckthorn, 0.5~3 part of the root of kudzu vine, 0.5~2 part of cassia seed, 0.1~0.8 part of aloe and 0.3~
2 parts of hawthorn;
The preparation method of the fermentation composition at least comprises the steps:
The fine powder that the functional oligose and Chinese medical extract of predetermined weight number are made is sterilized after mixing, after sterilization with
Probiotics powder agent is uniformly mixed, granulation, and the fermentation composition of powdery is made.
Probiotics and functional oligose reasonable combination are used, and both have mutual promoting action, can be doubled to enteron aisle
The regulatory function of flora, so that the effect based on gut flora plays more preferable strengthen immunity;And the traditional Chinese medicine ingredients added, enter
One step realizes effect of conditioning cardiovascular and cerebrovascular diseases.And powdery is made in fermentation composition, the later stage is easy in the production of implementation phase formula
Direct plunge into use or family oriented is used.
Further, functional oligose of the fermentation composition by parts by weight for 5~10 parts, 1~3 part prebiotic
Bacterium and 0.5~1 part of Chinese medical extract composition.
Further, Chinese medical extract is extracted by the raw material of following parts by weight and formed:0.9~1.2 part of matrimony vine, 1.2~
1.5 parts of mulberries, 0.6~1.1 part of sea-buckthorn, 1.2~1.5 parts of the root of kudzu vine, 1~1.5 part of cassia seed, 0.2~0.5 part of reed
Luxuriant growth and 0.9~1.2 part of hawthorn.
In such scheme, the functional oligose is selected from chitosan, xylo-oligosaccharide, kanjak mannan-oligosaccharides, sweet dew
Oligosaccharide, milk ketose, raffinose, soyabean oligosaccharides, FOS, galactooligosaccharide, lactosucrose, konjac mannan sugar,
One or more in trehalose, palatinose and inulin;
Bifidobacterium includes bifidobacterium longum (B.longum), short Bifidobacterium Bifidum (B.breve) (B.breve), bifidobacterium infantis
And the one or more in bifidobacterium lactis (B.ifidobacterium) (B.infantis);
Lactobacillus includes Lactobacillus rhamnosus (L.rhamnosus), Lactobacillus casei (Lactobacillus
Casei), lactobacillus acidophilus (L.acidophilus), Lactobacillus plantarum (Lactobacillus plantarum), the newborn bar of saliva
Bacterium (Lactobacillus salivarius), Lactobacillus helveticus (Lactobacillus helveticus), Bu Shi lactobacillus
(Lactobacillus buchneri), lactobacillus bulgaricus (Lactobacillus.Bulgaricus), the newborn bar of secondary cheese
One kind or several in bacterium (Lactobacillus paracasei) and lactobacillus reuteri (Lactobacillus reuteri)
Kind.
The preferred scheme of the fermentation composition of the present invention, it is 8~20 parts that the fermentation composition, which also includes parts by weight,
Resistant dextrin, 5~8 parts of phytosterol and 3~6 parts of γ-aminobutyric acid, resistant dextrin are used in conjunction with other components,
Further promote intestines peristalsis, keep intestinal health, and control the absorption of grease and sugar;Phytosterol has stronger to human body
Antiinflammatory action, with can suppress absorption of the human body to cholesterol, promote the katabolism of cholesterol, suppress the biochemistry of cholesterol
The effect such as synthesis, has obvious curative effect for heart disease of prophylactic treatment coronary atherosclerosis class etc.;And γ-aminobutyric acid
The reactivity of brain can be promoted, brain tonic and intelligence development, anti-epileptic delays brain aging function, can supplement human body inhibitory neurotransmitter, have
Good blood pressure reduction effect.The collaboration of this three's auxiliary material is used, and can further improve its effect for nursing one's health cardiovascular and cerebrovascular diseases.
Further, the functional oligose is the enzyme for not having to hydrolyze xylo-oligosaccharide in xylo-oligosaccharide, human gastrointestinal tract,
So it is preferably that Bifidobacterium is utilized that xylo-oligosaccharide, which can be directly entered big enteral, Bifidobacterium propagation is promoted to produce simultaneously a variety of
Organic acid, reduces enteron aisle pH value, suppresses harmful bacteria growing, probiotics is largely bred in enteron aisle;Available for treatment constipation and slowly
Property diarrhoea, protect liver, prevent and treat the effect of hypertension and artery sclerosis and anti-aging;
The probiotics is probiotics powder agent, is uniformly mixed by the pulvis of following parts by weight:Bifidobacterium 45~
60 parts, 20~25 parts of Lactobacillus rhamnosus, 12~16 parts of Lactobacillus casei, 14~18 parts of lactobacillus acidophilus and Lactobacillus plantarum
23~30 parts, the viable bacteria content of the probiotics powder agent is 2 × 108~8 × 108cfu/g;By all kinds of strains of reasonable disposition and
Consumption, optimizes the strain of this patent products'texture, can effectively shorten fermentation composition in the time for preparing plant enzyme, and can make
It prepares the stable plant enzyme of texture.
The source of above-mentioned all kinds of probiotics powder agents:It is the pulvis of commercially available all kinds of strains or by will be commercially available all kinds of
Strain is collected by centrifugation with after washing thalline, drying is prepared into active powder through Liquid Culture.
In another inventive technique scheme, fermentation composition of the invention also includes the yeast that parts by weight are 1~3 part
Bacterium, 1~3 part of acetobacter and 1~5 part of lactic acid bacteria;
The preparation method of the fermentation composition also includes:
(1) softwood is made in the fermentation composition of the powdery of 1/2 amount, is well mixed with the lactic acid bacteria of the parts by weight
Extrusion is carried out afterwards and makes ball, and capsule core is made;
(2) fermentation composition of the powdery of 1/2 amount is mixed with the saccharomycete and acetobacter of the parts by weight
It is even, softwood thing is made, it is standby;
(3) obtained capsule core is placed in the coating solution prepared in advance, be coated;
(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce
Pellet fermentation composition;
Wherein, the coating solution is by the adjacent benzene for the acroleic acid resin I No. I, 0.05-1 parts that parts by weight are 0.4-10 parts
Dicarboxylate and 1-3 parts of castor oil add the aqueous dispersion that appropriate purified water is configured to.
As above, the fermentation pill with double-layer structural, outer layer fermentation ball mixing composite bacteria -- saccharomycete and vinegar are prepared into
Acidfast bacilli, internal layer fermentation ball is mixed with lactic acid bacteria, the step of eliminating later stage multistage inoculation composite bacteria, improves what product was used
Convenience;And the time limit that internal layer pill starts dissolving can effectively be extended by by coating membrane separate between ectonexine, two are reached
Level inoculation fermentation effect, realizes deep layer liquid state fermentation.
The preparation method of the fermentation composition of powdery of the present invention, comprises the following steps:
1) preparation of Chinese medical extract:
The root of kudzu vine and cassia seed of the parts by weight are crushed respectively, 60 mesh sieves are crossed, root of kudzu vine powder and Cassia Seed is made;
By root of kudzu vine powder, the matrimony vine of Cassia Seed and predetermined weight part, mulberries, sea-buckthorn, aloe, hawthorn with 8 times of weight
Water, keep 4h at 80-90 DEG C of temperature, be then cooled to 40 DEG C, plus the alcohol reflux 4h that 2 times of weight concentrations are 75%, mistake
Filter, obtains the first filtrate;The water of 3 times of weight of filter residue is added, 2.5h is kept at 80-90 DEG C of temperature, is then cooled to 30 DEG C, mistake
Filter, the second filtrate;Merging filtrate, is concentrated under reduced pressure, is freeze-dried, and low-temperature grinding is thin into the Chinese medicine for being ground into 0.05-0.2mm
Powder;
2) by step 1) in traditional Chinese medicine fine powder mixed with emulsifying agent, water after carry out high speed shear with cutter, form particle
Particle diameter is less than 50 microns of Chinese medicine particulate;
3) by the functional oligose of predetermined weight number, resistant dextrin, phytosterol, γ-aminobutyric acid and step 2)
In Chinese medicine particulate uniformly mix, sterilized by heat exchanger, condition be 99 DEG C, 300s;
4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, is added after being well mixed dry
Method comminutor is granulated, and the fermentation composition of powdery is made, and sterile packing is produced.
A further object of the present invention is to provide a kind of plant ferment for being applied to cardiovascular and cerebrovascular diseases conditioning and its routine servicing
Element, is formulated, the preparation method of the plant enzyme comprises the following steps for personal or Homemade plant enzyme:
(a) powdery fermentation composition is prepared
(b) mixed material:1kg plant material is cleaned, is put into the sugar of 0.3~0.6kg weight in ferment bucket, adds 5
~6L pure water, be well mixed, while add weight be 20~50g the step of (a) prepare fermentation composition to ferment bucket
Interior, capping carries out lucifuge fermentation in fermentation temperature is 28~30 DEG C of environment;
(c) ferment:Fermentate in ferment bucket is carried out after stirring sooner or later once daily, 5~8d of fermentation, filtering, i.e.,
Obtain plant enzyme.
This method simple and fast, realizes the self-control of various functions plant enzyme, and this method preferably uses ferment bucket and made
Standby, ferment bucket is from market sale;Also the vessel of closed environment can be used to be prepared.
Another preferred scheme of the preparation method of plant enzyme of the present invention, the described method comprises the following steps:
A) fermentation composition of powdery is prepared
B) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight adds into plant material
Enter 10L pure water, be well mixed, stand after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extraction liquid;
C) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out
Lucifuge is fermented 1~2 month, obtains plant enzyme stoste;
Wherein inoculum concentration is 8~12%;Composite flora is saccharomyces cerevisiae, the newborn subspecies of Lactobacillus delbrueckii, Lactobacillus plantarum, leather
It is several in Lan Shi lactobacillus, lactobacillus acidophilus and acetobacter;
D) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L
Water and plant material 1kg, add fermentation composition prepared by 150ml plant enzyme stoste and 30g step a), in closed
Fermented in environment, fermentation temperature is 28~30 DEG C, is deflated 1~2 time daily, after 4~6d of fermentation, filtering produces plant ferment
Element.
The preferred scheme of the preparation method of another plant enzyme, the use of the fermentation composition also constructed for double-deck pellet
Method, the preparation method comprises the following steps:
I the fermentation composition of pellet) is prepared
II) the preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of 1kg weight is added into plant material, and
10L pure water is added, is well mixed, is stood after 1~2d of extraction, high-temperature sterilization is carried out, filtering obtains plant extraction liquid;
III) the preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g,
8L water and plant extraction liquid 1kg, add 30g step II) prepare pellet fermentation composition, in closed environment carry out
Fermentation;Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily;Ferment after 1~2d, insertion conduit is passed through air, daily ventilation
2-4 times, each 10-30min, and control fermentation temperature to be 35~37 DEG C;Filtered after 5~7d of fermentation, produce plant enzyme.
This method simple and fast, realizes the self-control of various functions plant enzyme, and fermentation process is simply deep, once-through operation
Realize second order fermentation, or even multistage fermentation;The plant enzyme rich in nutrition content being prepared into, human absorptivity is high, further carries
Its high effect.
The present invention has the following advantages that compared with prior art:
The fermentation composition that the present invention is developed is novel fermentation product, is for preparing ferment " fermentation bacterium bag ", improveing
Direct addition probiotics prepares the traditional handicraft of plant enzyme, and the bacterium bag is easy to use, can directly with the bacterium bag of pulvis in city
Sold on face, can also put into the industrialized production of all kinds of plant enzymes, realize matching somebody with somebody i.e. with the work(with diversification for plant enzyme
Energy property configuration, is greatly enhanced the preparation efficiency of plant enzyme.
The traditional Chinese medicinal components added in the fermentation composition of the present invention have its physiologically active and health-care effect, while being prebiotic
Bacterium supplements the nutrients in fermentation, and the traditional Chinese medicine ingredients with higher practical value, and the present invention can effectively nurse one's health cardiovascular and cerebrovascular disease
Disease;Rationally, curative effect is clear and definite, the effect with Synergistic for compatibility between probiotics, functional oligose and Chinese medicine each component.
The plant enzyme of the present invention meets requirement and the development trend of health products, and plant enzyme can be produced largely to be had to human body
The comprehensive plant enzyme of benefit, metabolism and function adjustment of these enzymes to human body plays an important role, while also containing vitamin, mineral
The nutritional ingredients such as matter, amino acid, organic acid, oligosaccharide.The present invention is fermented by adding above-mentioned fermentation composition, fermentation group
Traditional Chinese medicine ingredients in compound are changed through probiotics, are become small molecule by macromolecular, are easily absorbed by the body;The addition of probiotics can
Toxicity can be decomposed by improving in Chinese medicine taste, fermentation process, realization really has no toxic side effect, while prepared by the fermentation composition
Plant enzyme improves drug effect compared to traditional Chinese medicine, and the blood pressure reduction effect of energy highly significant can promote human amino acid's generation again
The balance thanked, adjusts gut flora.The plant ferment is suitably used with cardiovascular and cerebrovascular crowd and elderly population, is used as daily drink
Product are taken, and lipid-metabolism is improved from the angle effected a permanent cure, and are improved body immunity etc., are realized the purpose of health care.
In addition, the technical process that the direct addition of fermentation composition prepares plant enzyme is simple, it is with low cost, it is easy to accomplish big
Large-scale production, compared to prior art, shortens the time for preparing and fermenting, and safe preparation process.
The source of the Chinese medicine material composition of the present invention:
Matrimony vine:This product is plant of Solanaceae lycium barbarum Lycium barbarum L. dry mature fruit.
Mulberries:This product is moraceae plants mulberry Morus alba L. drying fruit ear.
Sea-buckthorn:This strain Mongols, Tibetan conventional crude drugs.For Elaeangnaceae hippophae plant sea-buckthorn Hippophae
Rhamnoides L. dry mature fruit.
The root of kudzu vine:This product is legume pueraria lobata Pueraria lobata (Willd.) Ohwi or sweet kudzu Pueraria
Thomsonii Benth. dry root.
Cassia seed:This product is legume Cassia Cassia obtusifolia L. or little Cassia tora Cassia tora L.
Dry mature seed.
Aloe:This product is liliaceous plant aloe barbadensis Miller Aloe barbadensis Miller, Aloe ferox Miller
Aloeferox Miller or other belong to the dry product of concentrated juice of kindred plant leaf together.
Hawthorn:This product is rosaceous plant large-fruited Chinese hawthorn Crataegus pinnatifida Bge.var.major
N.E.Br. or hawthorn Crataegus pinnatifida Bge. dry mature fruit.
Embodiment
The fermentation composition of embodiment 1
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Xylo-oligosaccharide 80g;
Probiotics powder agent:24g, wherein containing 4.5 parts of Bifidobacterium, 2 parts of Lactobacillus rhamnosus, cheese in per g probiotic powders
1.6 parts of lactobacillus, 1.8 parts of lactobacillus acidophilus, 2.3 parts of Lactobacillus plantarum;
Traditional Chinese medicine ingredients:8g, is formed wherein being extracted per g Chinese medical extracts by the raw material of following portions by weight:1.2 parts of matrimony vine,
1.2 parts of 1.5 parts of mulberries, 1.1 parts of sea-buckthorn, 1.5 parts of the root of kudzu vine, 1.5 parts of cassia seed, aloe 0.5 and hawthorn;
Resistant dextrin 80g, phytosterol 40g, γ-aminobutyric acid 24g;
Preparation method:
1) preparation of Chinese medical extract:
The root of kudzu vine and cassia seed of the parts by weight are crushed respectively, 60 mesh sieves are crossed, root of kudzu vine powder and Cassia Seed is made;
By root of kudzu vine powder, the matrimony vine of Cassia Seed and predetermined weight part, mulberries, sea-buckthorn, aloe, hawthorn with 8 times of weight
Water, keep 4h at 80-90 DEG C of temperature, be then cooled to 40 DEG C, plus the alcohol reflux 4h that 2 times of weight concentrations are 75%, mistake
Filter, obtains the first filtrate;The water of 3 times of weight of filter residue is added, 2.5h is kept at 80-90 DEG C of temperature, is then cooled to 30 DEG C, mistake
Filter, the second filtrate;Merging filtrate, is concentrated under reduced pressure, is freeze-dried, and low-temperature grinding is thin into the Chinese medicine for being ground into 0.05-0.2mm
Powder;
2) by step 1) in traditional Chinese medicine fine powder mixed with emulsifying agent, water after carry out high speed shear with cutter, form particle
Particle diameter is less than 50 microns of Chinese medicine particulate;
3) by the functional oligose of predetermined weight number, resistant dextrin, phytosterol, γ-aminobutyric acid and step 2)
In Chinese medicine particulate uniformly mix, sterilized by heat exchanger, condition be 99 DEG C, 300s;
4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, is added after being well mixed dry
Method comminutor is granulated, and the fermentation composition of powdery, sterile pack, every bag of 30g is made.
The fermentation composition of embodiment 2
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Xylo-oligosaccharide 100g
Probiotics powder agent:20g, wherein containing 6 parts of Bifidobacterium, 2.2 parts of Lactobacillus rhamnosus, cheese in per g probiotic powders
1.2 parts of lactobacillus, 1.4 parts of lactobacillus acidophilus, 3 parts of Lactobacillus plantarum;Traditional Chinese medicine ingredients:10g, wherein per g Chinese medical extracts under
The raw material extraction for stating parts by weight is formed:0.9 part of matrimony vine, 1.2 parts of mulberries, 0.6 part of sea-buckthorn, 1.2 parts of the root of kudzu vine, 1 part of cassia seed, reed
0.9 part of 0.2 part of luxuriant growth and hawthorn.
Preparation method:
1) preparation of Chinese medical extract:
The root of kudzu vine and cassia seed of the parts by weight are crushed respectively, 60 mesh sieves are crossed, root of kudzu vine powder and Cassia Seed is made;
By root of kudzu vine powder, the matrimony vine of Cassia Seed and predetermined weight part, mulberries, sea-buckthorn, aloe, hawthorn with 8 times of weight
Water, keep 4h at 80-90 DEG C of temperature, be then cooled to 40 DEG C, plus the alcohol reflux 4h that 2 times of weight concentrations are 75%, mistake
Filter, obtains the first filtrate;The water of 3 times of weight of filter residue is added, 2.5h is kept at 80-90 DEG C of temperature, is then cooled to 30 DEG C, mistake
Filter, the second filtrate;Merging filtrate, is concentrated under reduced pressure, is freeze-dried, and low-temperature grinding is thin into the Chinese medicine for being ground into 0.05-0.2mm
Powder;
2) by step 1) in traditional Chinese medicine fine powder mixed with emulsifying agent, water after carry out high speed shear with cutter, form particle
Particle diameter is less than 50 microns of Chinese medicine particulate;
3) by the functional oligose and step 2 of predetermined weight number) in Chinese medicine particulate uniformly mix, pass through heat exchanger
Sterilized, condition is 99 DEG C, 300s;
4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, is added after being well mixed dry
Method comminutor is granulated, and the fermentation composition of powdery, sterile packed, every bag of 30g is made.
The fermentation composition of embodiment 3
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Xylo-oligosaccharide 60g
Probiotics powder agent:2g, wherein 4.4 parts of Bifidobacterium, 2.3 parts of Lactobacillus rhamnosus, cheese breast in per g probiotic powders
1.4 parts of bacillus, 1.6 parts of lactobacillus acidophilus, 2.6 parts of Lactobacillus plantarum;
Traditional Chinese medicine ingredients:0.2g, is formed wherein being extracted per g Chinese medical extracts by the raw material of following portions by weight:Matrimony vine 0.3
0.9 part of part, 0.5 part of mulberries, 0.1 part of sea-buckthorn, 0.5 part of the root of kudzu vine, 0.5 part of cassia seed, 0.2 part of aloe and hawthorn;
Resistant dextrin 160g, phytosterol 120g, γ-aminobutyric acid 120g.
Preparation method:Prepared according to the preparation method of embodiment 1, pulvis, every bag of 30g is made.
The fermentation composition of embodiment 4
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Xylo-oligosaccharide 220g
Probiotics powder agent:50g, wherein 5 parts of Bifidobacterium, 2.4 parts of Lactobacillus rhamnosus, cheese breast in per g probiotic powders
1.3 parts of bacillus, 1.5 parts of lactobacillus acidophilus, 2.8 parts of Lactobacillus plantarum;
Traditional Chinese medicine ingredients:100g, is formed wherein being extracted per g Chinese medical extracts by the raw material of following portions by weight:2 parts of matrimony vine,
2 parts of 3 parts of mulberries, 1.6 parts of sea-buckthorn, 3 parts of the root of kudzu vine, 2 parts of cassia seed, 0.8 part of aloe and hawthorn;
Preparation method:Prepared according to the preparation method of embodiment 2, pulvis, every bag of 30g is made.
The fermentation composition of embodiment 5
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Chitosan 50g, soy oligosaccharides Icing Sugar 80g, FOS 70g;
Probiotics powder agent:20g, wherein 4 parts of bifidobacterium longum, 1.8 parts of Lactobacillus casei, saliva breast in per g probiotic powders
0.9 part of bacillus, 1.5 parts of lactobacillus acidophilus, Lactobacillus plantarum 2.2;
Traditional Chinese medicine ingredients:50g, is formed wherein being extracted per g Chinese medical extracts by the raw material of following portions by weight:1.2 parts of matrimony vine,
0.9 part of 2.1 parts of mulberries, 1.3 parts of sea-buckthorn, 2.1 parts of the root of kudzu vine, 2 parts of cassia seed, aloe 0.2 and hawthorn;
Resistant dextrin 200g, phytosterol 80g, γ-aminobutyric acid 50g.
Preparation method:Prepared according to the preparation method of embodiment 1, pulvis, every bag of 30g is made.
The fermentation composition of embodiment 6
The prescription of the fermentation composition of the present embodiment is:
Functional oligose:Kanjak mannan-oligosaccharides 53g, mannan-oligosaccharides 23g, trehalose 15g;Probiotics powder agent:
10.1g, wherein containing 2.3 parts of bifidobacterium breve, 2 parts of bifidobacterium lactis, 2.4 parts of Lactobacillus rhamnosus, Switzerland in per g probiotic powders
9 parts of 1.3 parts of lactobacillus, 1 part of Bu Shi lactobacillus, 1.7 parts of lactobacillus bulgaricus and lactobacillus paracasei;
Traditional Chinese medicine ingredients:4.55g;Extracted and formed by the raw material of following portions by weight wherein per g Chinese medical extracts:Matrimony vine 1.4
Part, 2.6 parts of mulberries, 0.5 part of sea-buckthorn, 1.6 parts of the root of kudzu vine, 0.8 part of the stem of noble dendrobium and cassia seed 2.1g
Resistant dextrin 41.8g, phytosterol 20.1g, γ-aminobutyric acid 8.6g
Preparation method:Prepared according to the preparation method of embodiment 1, pulvis, every bag of 30g is made.
The fermentation composition of embodiment 7
The prescription of the fermentation composition of the present embodiment is:
The pulvis fermentation composition 32g that embodiment 1 is made;Saccharomycete 1g;Acetobacter 1g;Lactic acid bacteria 1g;Coating solution by
Acroleic acid resin II 20g, diethyl phthalate 2.5g and the appropriate purifying of castor oil 0.5g additions are prepared;
Preparation method:
(1) softwood is made in the pulvis fermentation composition of 1/2 amount, after being well mixed with the lactic acid bacteria of the parts by weight
Carry out extrusion and make ball, capsule core is made;
(2) the pulvis fermentation composition of 1/2 amount is well mixed with the saccharomycete and acetobacter of the parts by weight,
Softwood thing is made, it is standby;
(3) obtained capsule core is placed in the coating solution prepared in advance, be coated;
(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce
Pellet fermentation composition, packaging.
Wherein obtained pellet diameters are 4-7mm, a diameter of 2-5mm of capsule core;It is preferred that pellet diameters 5mm, capsule core is a diameter of
2.8mm。
The fermentation composition of embodiment 8
The prescription of the fermentation composition of the present embodiment is:
The pulvis fermentation composition 23.1g that embodiment 3 is made;Saccharomycete 3g;Acetobacter 3g;Lactic acid bacteria 5g;Coating solution
Prepared by the appropriate purifying of acroleic acid resin II 10g, diethyl phthalate 1g and castor oil 3g addition;
Preparation method:
(2) softwood is made in the pulvis fermentation composition of 1/2 amount, after being well mixed with the lactic acid bacteria of the parts by weight
Carry out extrusion and make ball, capsule core is made;
(2) the pulvis fermentation composition of 1/2 amount is well mixed with the saccharomycete and acetobacter of the parts by weight,
Softwood thing is made, it is standby;
(3) obtained capsule core is placed in the coating solution prepared in advance, be coated;
(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce
Pellet fermentation composition, packaging.
Wherein obtained pellet diameters are 4-7mm, a diameter of 2-5mm of capsule core;It is preferred that pellet diameters 5mm, capsule core is a diameter of
2.8mm。
The plant enzyme of embodiment 9
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 1;
B) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight adds into plant material
Enter 10L pure water, be well mixed, stand after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extraction liquid;
C) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out
Lucifuge is fermented 2 months, obtains plant enzyme stoste;
Wherein inoculum concentration is 12%;Composite flora is Lactobacillus plantarum, gram lactobacillus, lactobacillus acidophilus and acetic acid
In bacillus;
D) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L
Water and plant material 1kg, add fermentation composition prepared by 150ml plant enzyme stoste and 30g step a), in closed
Fermented in environment, fermentation temperature is 28~30 DEG C, is deflated 1~2 time daily, after fermentation 6d, filtering produces plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g, and clean section is used.
Organoleptic parameters:The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 10
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 2;
B) preparation of plant extraction liquid:1kg plant material is cleaned, the sugared to plant material of 0.3~0.6kg weight is added
In, and 5~6L pure water is added, it is well mixed, stands after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extract
Liquid;
C) mixing and initial fermentation:Add plant of fermentation composition of the weight for 60g step a) preparations into step b)
In thing extract solution, mixing is placed in closed environment, keeps 28 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid;
D) zymotic fluid carries out after-ripening 7d in closed environment, filtering, produces plant enzyme.
Wherein, plant material selects Kiwi berry 100g, strawberry 90g, hawthorn 40g, mango 220g, carrot 80g, ternip
140g, Chinese cabbage 150g, agaric 80g and mushroom 100g.
Organoleptic parameters:The plant enzyme uniform color, between buff and brown, having, strong fruity and fermentation are fragrant
Gas, mellow in taste, comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 11
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 3;
B) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight adds into plant material
Enter 10L pure water, be well mixed, stand after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extraction liquid;
C) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out
Lucifuge is fermented 1 month, obtains plant enzyme stoste;
Wherein inoculum concentration is 8~12%;Composite flora is saccharomyces cerevisiae, the newborn subspecies of Lactobacillus delbrueckii, Lactobacillus plantarum, leather
It is several in Lan Shi lactobacillus, lactobacillus acidophilus and acetobacter;
D) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L
Water and plant material 1kg, add fermentation composition prepared by 150ml plant enzyme stoste and 30g step a), in closed
Fermented in environment, fermentation temperature is 28~30 DEG C, is deflated 1~2 time daily, after fermentation 4d, filtering produces plant enzyme.
Wherein, plant material selects passion fruit 120g, citrus 200g, grape 100g, Chinese yam 50g, pumpkin 220g, celery
40g, water spinach 50g, bean sprouts 50g, sea-tangle 60g, seaweed 40g and asparagus 70g.
Organoleptic parameters:The plant enzyme uniform color, in dark brown, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 12
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 6;
B) preparation of plant extraction liquid:1kg plant material is cleaned, the sugared to plant material of 0.3~0.6kg weight is added
In, and 5~6L pure water is added, it is well mixed, stands after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extract
Liquid;
C) mixing and initial fermentation:The fermentation composition for the step a) preparations that weight is 30~60g is added into step b)
Plant extraction liquid in, mixing is placed in closed environment, keeps 30 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid;
D) zymotic fluid carries out after-ripening 7d in closed environment, filtering, produces plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
Organoleptic parameters:The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 13
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared according to the fermentation composition of embodiment 1;
B) mixed material:1kg plant material is cleaned, is put into the sugar of 0.3~0.6kg weight in ferment bucket, addition 5~
6L pure water, is well mixed, while add in fermentation composition to the ferment bucket that weight is prepared for 20~50g step a),
Capping, lucifuge fermentation is carried out in fermentation temperature is 28~30 DEG C of environment;
C) ferment:Fermentate in ferment bucket is carried out after stirring sooner or later once daily, 4~6d of fermentation, filtering is produced
Plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
Organoleptic parameters:The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
The plant enzyme of embodiment 14
The plant enzyme of the present embodiment is prepared from by following methods:
I the fermentation composition of pellet) is prepared according to embodiment 7
II) the preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of 1kg weight is added into plant material, and
10L pure water is added, is well mixed, is stood after 1~2d of extraction, high-temperature sterilization is carried out, filtering obtains plant extraction liquid;
III) the preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g,
8L water and plant extraction liquid 1kg, add 30g step II) prepare pellet fermentation composition, in closed environment carry out
Fermentation;Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily;Ferment after 1~2d, insertion conduit is passed through air, daily ventilation
2-4 times, each 10-30min, and control fermentation temperature to be 35~37 DEG C;Filtered after 5~7d of fermentation, produce plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
Organoleptic parameters:The plant enzyme uniform color, in bronzing, has strong fruity and the fragrance that ferments, mellow in taste,
Comfortable acid, entrance is not stimulated, and pleasant impression is sweet.
Observation to the pellet fermentation composition fermentation process of embodiment 7:Pellet fermentation composition during the fermentation, ball
The outer layer component of grain dissolves rapidly in the solution, and subsequent capsule core dissolving is slow, and coating membrane fades away after about 1-2d, and capsule core is completely molten
Solution.
The fermentation composition of comparative examples 1
The prescription of the fermentation composition of the present embodiment is::
Functional oligose:Xylo-oligosaccharide 80g;
Probiotics powder agent:24g, wherein containing 4.5 parts of Bifidobacterium, 2 parts of Lactobacillus rhamnosus, cheese in per g probiotic powders
1.6 parts of lactobacillus, 1.8 parts of lactobacillus acidophilus, 2.3 parts of Lactobacillus plantarum;
Resistant dextrin 80g, phytosterol 40g, γ-aminobutyric acid 24g;
Preparation method:
By the functional oligose of predetermined weight number, probiotics powder agent, resistant dextrin, phytosterol, γ-aminobutyric acid
10~15min of uniform mixing, dry granulating machine granulation is added by mixed powder, and the pulvis of fermentation composition, pack, every bag is made
30g。
The plant enzyme of comparative examples 2
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of fermentation composition:Prepared by the fermentation composition of comparative examples 1;
B) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight adds into plant material
Enter 10L pure water, be well mixed, stand after extraction 2d, carry out high-temperature sterilization, filtering obtains plant extraction liquid;
C) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carried out
Lucifuge is fermented 1~2 month, obtains plant enzyme stoste;
Wherein inoculum concentration is 12%;Composite flora is Lactobacillus plantarum, gram lactobacillus, lactobacillus acidophilus and acetic acid
In bacillus;
D) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L
Water and plant material 1kg, add fermentation composition prepared by 150ml plant enzyme stoste and 30g step a), in closed
Fermented in environment, fermentation temperature is 28~30 DEG C, is deflated 1~2 time daily, after fermentation 6d, filtering produces plant enzyme.
Wherein, plant material selects apple 500g, soybean 200g and tomato 300g.
The plant enzyme of comparative examples 3
The plant enzyme of the present embodiment is prepared from by following methods:
A) preparation of plant extraction liquid:1kg plant material is cleaned, the sugared to plant material of 0.3~0.6kg weight is added
In, and 5~6L pure water is added, it is well mixed, stands after 1~2d of extraction, carry out high-temperature sterilization, filtering obtains plant extract
Liquid;
B) mixing and initial fermentation:It is inoculated with plant extraction liquid of the compound probiotic into step b), mixing is placed in closed
Environment, keeps 30 DEG C of fermentation temperature, carries out lucifuge fermentation, obtains initial fermentation liquid;
C) zymotic fluid carries out after-ripening 7d in closed environment, takes Partial fermentation liquid to filter, does organoleptic parameters;Continue after-ripening
14d, takes Partial fermentation liquid to filter, does organoleptic parameters.
Wherein, compound probiotic is Bifidobacterium, Lactobacillus rhamnosus, Lactobacillus casei, lactobacillus acidophilus and plant
Lactobacillus;Inoculum concentration is 8% (mass percent);
Plant material selects apple 500g, soybean 200g and tomato 300g.
The organoleptic parameters of zymotic fluid after 7 days:The plant enzyme color and luster is in bronzing, and tissue mobility is big, entrance slightly has
Stimulation, sweet taste are heavier;The organoleptic parameters of zymotic fluid after 14 days:The plant enzyme uniform color, in bronzing, there is strong
Fruity is not stimulated with fermentation fragrance, mellow in taste, comfortable acid, entrance, and pleasant impression is sweet.Understand, plant prepared by conventional method
The fermentation time that ferment needs is longer than the fermentation time of embodiment of the present invention plant enzyme.
Oligosaccharide content detection in the plant enzyme of test example 1
1st, instrument
Using Japanese Shimadzu LC-4A high performance liquid chromatographs, PID-2AS differential refraction detectors, WatersTCM post constant temperature
Case, WATERS745 integrators.
2nd, chromatography experiment condition
(1) column temperature:Selected column temperature is 40 DEG C and is used as experimental condition.
(2) mobile phase:It is fine using second:The ratio of water is respectively 65:35;70:30;75:25 and 80::20 4 kinds combine into
Row experimental study.Sucrose, gossypose, the retention time of stachyose are respectively 7.14,11.05,17.45 points, whole separation determination
Process can be completed within the time of 20 minutes.
(3) chromatographic condition:Chromatographic column:Waters Spherisorb 5μmNH24.6×250mm;Column temperature:40℃;Flowing
Phase:Second is fine:Water is 7:3;Flow velocity:1ml/min;Differential refraction detector;Range is 0.5.
3rd, the processing of test sample
The ferment solution of embodiment 9 is dissolved with 4 parts of alcohol constant volumes and fully shaking, filtrate passes through 0.45 after filter paper filtering
μm organic membrane filtration after upper machine determine.
(2) after sample being dissolved with 3 parts of water, dissolved with 7 parts of fine constant volumes of second and fully shaking, filtrate passes through after filter paper filtering
Upper machine is determined after 0.45 μm of organic membrane filtration.
4th, result and analysis
The measure of 4.1 standard curves
(1) preparation of standard liquid:0.2500g gossyposes and stachyose are accurately weighed respectively, are settled to water dissolving
10ml, concentration is 20mg/ml storing solution;Then be configured to concentration be respectively 2.5,5.0,7.5,10.0,15.0mg/ml makes
Use liquid.Glucose, fructose, sucrose are configured to 2mg/ml standard liquid respectively, qualitative for being carried out to these three sugar.
(2) draw respectively and use liquid 10ul, the chromatographic condition in 2.2 (3) determines peak height.As a result show, gossypose is returned
Equation is y=-0.2566+0.1204x, and coefficient correlation is r=0.9591;Stachyose is y=0.0996+0.1314x, phase relation
Number is r=0.9844.It is proportionate between concentration and peak height, correlation is good, is quantitative determined available for external standard method.
The measure of 4.2 samples
(1) it is put into after 150ml samples are dissolved with 40ml85% ethanol in ultrasonic wave and vibrates 10min, is used after taking-up
85% ethanol is transferred to constant volume in 50ml volumetric flasks, is filtered after mixing with filter paper, and filtrate is surveyed by upper machine after 0.45 μm of filter membrane
It is fixed.
(2) it is fine for second through the selected mobile phase of experiment:Water (7:3), glucose can not be had with fructose on this condition
Effect separation, retention time is all 6.04, but sucrose and raffinose, stachyose good separating effect, its retention time is respectively 7.54,
11.32 and 18.09min.
Measurement result is shown in Table 1:
Raffinose and stachyose measurement result (unit in the plant enzyme of table 1:mg/100ml)
As a result show, the plant macromolecular sugar in plant enzyme has been converted into small molecule oligosaccharide, and high conversion rate;Wood
Sugar and raffinose can turn into the foodstuff of enteral beneficial bacterium Bifidobacterium Bifidum, can fast breeding Bifidobacterium Bifidum, effectively suppress harmful
Bacterium breeds, and promotes intestines peristalsis, thus, it is possible to prevent a large amount of generations of harmful substance in enteron aisle, mitigates burden of liver.
The viable bacteria survival detection of the fermentation composition of test example 2
Fermentation composition prepared by the embodiment of the present invention 1 and embodiment 7, be prepared into sterilized water viable count for 2 ×
108CFU/mL solution to be measured, is saved backup in 4 DEG C;
The 10mL kept the solution to be measured of going bail for is injected into test tube 1, and 10 are diluted to step by step using ten times-8, take 1mL to dilute
The MRS agar mediums that 45 DEG C are cooled to after sterilizing are poured on flat board (sterilizing), shaken up rapidly on flat board by liquid.Again will
The test tube 2 of solution to be measured equipped with 10mL, which is placed in 80-90 DEG C of water-bath, heats 15-25min, takes the solution to be measured after heating to enter
Row ten is diluted to 10 step by step again-8, 1mL dilutions are taken on flat board, and the MRS agar mediums that 45 DEG C are cooled to after sterilizing are poured into
Shake up on flat board (sterilizing) and rapidly.Flat board before finally heating and after heating cultivates 24h under the conditions of 35 DEG C, calculates
Number of viable before and after heating.
As a result show, Viable detection is respectively 88.7% and 88.1%.
The viable bacteria survival detection of the plant enzyme of test example 3
1st, simulate the gastric juice and the resistance test of intestinal juice
100g/L hydrochloric acid 16.4mL plus distilled water diluting is taken, it is respectively 1.5,2.5 and 3.5 to make pH value, take the dilute salt of 100mL
Acid solution, is separately added into 1g pepsins, it is fully dissolved, and obtains simulate the gastric juice, and miillpore filter degerming (0.22 μm) is standby.Take
Potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, and pH value is adjusted to 6.8 with 0.1moL/L sodium hydroxide solutions;Separately take tryptose
Enzyme 10g, the 100mL that adds water makes dissolving, after two liquid are mixed, is diluted with water to 1000ml, obtains simulated intestinal fluid, miillpore filter is degerming
(0.22 μm) is standby.
The plant enzyme solution of embodiment 9 that 1mL is kept and embodiment 14 is taken to be added in 9mL simulate the gastric juice respectively
(i.e. ten times dilute step by step), and rapid fully mixing on the oscillator, are subsequently placed in 30-45 DEG C of quiescent culture 2-4h.Exist respectively
Nutrient solution is taken out when 1h, 2h, 3h, 4h and remaining viable count is counted immediately, is compared, as a result shows with former viable count,
Viable detection is respectively 97.4% and 98.7%.Then each 1mL of nutrient solution that different time is digested in simulated gastric fluid is taken, point
It is not inoculated in the simulated intestinal fluid that 9mL pH value is 6.8, is placed in 30-45 DEG C of quiescent culture 2-4h, and taken respectively in 0,3,6,24h
Sample, determines its viable count, is compared with former viable count, and it is 98% as a result to show Viable detection.
The evaluation test that the plant enzyme of test example 4 influences on hyperlipidemia ratses
1st, to the influence of hyperlipemia lipid metabolism in rats level
1.1 test method:
1) model is set up:
High lipid food composition and preparation:3% cholesterol, 10% lard, 5% dried hen egg yolk, 0.3% sodium taurocholate, 0.2% first
Base thiouracil, 81.5% basal feed.10kg high lipid foods add 1~2kg white sugar.In proportion first by cholesterol, dried hen egg yolk,
In sodium taurocholate, propylthiouracil, white sugar ground and mixed, the lard for then adding warm, then mix thoroughly with basal feed, produce.
Modeling:82 SD rats normal diets are fed one week.Random selection 12 is only used as blank group mouse, mark of weighing, and gives
Normal diet is given to feed.Remaining 70 mouse is weighed mark, gives high lipid food nursing, and equal free water is fed 4 weeks and carries out height altogether
The foundation of pionemia model.
Record starts the rat body weight of surrounding after modeling respectively, and in the last eyeground vein clump of 4th week take 1.5~2ml of blood in
In centrifuge tube, stand, 3500rmin-115min is centrifuged, serum is separated, its Diagnostic Value of Fasting Serum is determined with semi-automatic biochemical analyzer
Middle T-CHOL (TC), triglycerides (TG), LDL-C (LDL-C), HDL-C (HDL-
C content).When T-CHOL (TC), triglycerides (TG) content are obviously higher than blank group in serum, illustrate high fat of blood animal
Model is successfully prepared.
2) it is administered
Next day starts plant enzyme of the gavage to embodiment 9 and embodiment 14, respectively with high, medium and low amount group respectively with
1.70ml/kg, 0.850ml/kg, 0.285ml/kg gastric infusion, totally 20 days.Continue to give high lipid food during experiment.Yu Mo
After secondary administration 1 hour, socket of the eye internal jugular vein clump takes blood 2.5ml, 4 DEG C of standings, 3500rmin-115min is centrifuged, serum is separated, with half
Automatic biochemistry analyzer detection T-CHOL (TC), triglycerides (TG) level and Apolipoprotein A1 (ApoA1) and load fat egg
White B (ApoB) level.
1.2 result of the test
It the results are shown in Table 2.
The influence that table 2 is metabolized to Hyperlipidemia
As a result show, each administration group can significantly reduce T-CHOL in serum (TC), triglycerides compared with model group
(TG), the content of LDL-C (LDL-C) and apolipoprotein B (ApoB), hence it is evident that increase HDL courage
Sterol (HDL-C) and Apolipoprotein A1 (ApoA1) content.
2nd, to the influence of hyperlipemia rat blood rheology index
Experimental animal is grouped and is administered ibid.In last dose 1 hour, abdominal aortic blood, sent and clinical laboratory examines.Knot
Really each administration group is compared WBV reduction, plasma viscosity reduction, packed cell volume reduction, erythrocyte sedimentation rate reduction, explanation with model group
It can suppress erythrocyte aggregation, reduce erythrocyte fragility, strengthen its morphotropism.
Conclusion (of pressure testing):The improvement that changes of the plant enzyme to Hyperlipemia model rat indices of the present invention has with
Therapeutic action, plant enzyme effect of embodiment 14 is more notable.
Evaluation of the plant enzyme of test example 5 to rats with myocardial ischemia is tested
1st, model is set up
Myocardial infarction model is replicated according to literature method.Intraperitoneal injection of anesthesia is used with 1% yellow Jackets (0.04g/kg),
Dorsal position is fixed on operating table.Record standard II lead electrocardiogram, row tracheotomy is connected with breathing apparatus, opens chest
Exposure heart, the left room branch of coronary artery is passed through between sustainer circular cone and left auricle of heart with surgical thread, ligatures arteria coroaria sinistra (sham-operation
A group threading is not ligatured), heart resets, and rapid after drum lung to close thoracic cavity, penicillin is embrocated in wound, prevents infected wound.Close
After chest observe rats breathing recovery situation, if any autonomous respiration can off line put back to mouse cage.There is back of a bow lift with ECG ST section
A height of myocardial ischemia modeling success.Chronic myocardial ischemia model is formed after four weeks.
2nd, it is grouped
Rats with myocardial ischemia after screening is uniformly divided into 8 groups, blank group, model group, implementation at random in addition to sham-operation group
The high dose group of example 9, the middle dose group of embodiment 9, the low dose group of embodiment 9, the high dose group of embodiment 14, the middle dose group of embodiment 14
And the low dose group of embodiment 14, every group 10, totally 80, raise in the lump.Administration group gavage gives the embodiment 9 of corresponding dosage
With the plant enzyme solution of embodiment 14, blank group, model group gavage give the distilled water of equivalent, once a day, totally 20 days.
2) to the influence of myocardial metabolism product
Each group rat was in last dose 1 hour, and eyeball takes blood, and detects blood with 756PC types ultraviolet-uisible spectrophotometer
Clear lactic acid (LD), free fatty (NEFA), Na-KATP enzyme indexs.Record data.
As a result each administration group is compared with model group group, the rat atpase activity reduction of plant enzyme of the present invention, lactic acid and trip
From content of fatty acid reduction.
3) to the influence of hemodynamic index
Rat is lain on the back and is fixed on operating table in last dose 1 hour by each group rat, cuts skin of neck, and separation is right
Conduit containing 0.1% liquaemin liquid is inserted right carotid artery, through right common carotid artery by side arteria carotis communis, row arterial cannulation
Left ventricle is inserted, whether the change of pressure figure according to display enters ventricle, cardiac catheter connection pressure to judge to be intubated
Transducer, inputs a signal into physiograph, and recorded heart rate (HR), left ventricular systolic pressure (LVSP), left ventricular pressure are most
Big climbing speed (+dp/dtmax), left ventricular diastolic pressure (LVDp), left ventricular end-diastolic pressure (LVDEp), left ventricular pressure
Maximum fall off rate (- dP/dtmax).Record data, is shown in Table 3.
Influence of the table 3 to hemorheology
As a result each administration group is compared with model group, and each administration group of the present composition can make heart rate tend to normal, left room
Systolic pressure rise, the reduction of left room diastolic pressure, the maximum climbing speed rise in left room, the maximum fall off rate rise in left room, last diastole eventually
Pressure drop is low, wherein the effect for drinking the plant enzyme of embodiment 14 is more notable.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that:Not
In the case of departing from the principle and objective of the present invention a variety of change, modification, replacement and modification can be carried out to these embodiments, this
The scope of invention is limited by claim and its equivalent.
Claims (10)
1. a kind of fermentation composition for preparing the plant enzyme suitable for cardiovascular and cerebrovascular diseases conditioning and its routine servicing, its feature exists
In the fermentation composition is direct putting type plant enzyme fermenting agent, and the fermentation composition includes the component of following parts by weight:
0.01~10 part of 3~22 parts of functional oligose, 0.1~5 part of probiotics and Chinese medical extract,
Wherein, the probiotics is one or more of jellies in Bifidobacterium, lactobacillus and streptococcus thermophilus
Dry powder;
The Chinese medical extract is extracted by the raw material of following parts by weight and formed:0.3~2 part of matrimony vine, 0.5~3 part of mulberries,
0.1~1.6 part of sea-buckthorn, 0.5~3 part of the root of kudzu vine, 0.5~2 part of cassia seed, 0.1~0.8 part of aloe and 0.3~2 part
Hawthorn;
The preparation method of the fermentation composition at least comprises the steps:
The fine powder that the functional oligose and Chinese medical extract of predetermined weight number are made is sterilized after mixing, after sterilization with it is prebiotic
Bacteria powder is uniformly mixed, granulation, and the fermentation composition of powdery is made.
2. fermentation composition as claimed in claim 1, it is characterised in that the fermentation composition is 5~10 by parts by weight
The functional oligose, 1~3 part of probiotics and 0.5~1 part of Chinese medical extract composition of part;
The Chinese medical extract is extracted by the raw material of following parts by weight and formed:0.9~1.2 part of matrimony vine, 1.2~1.5 parts of mulberry
Shen, 0.6~1.1 part of sea-buckthorn, 1.2~1.5 parts of the root of kudzu vine, 1~1.5 part of cassia seed, 0.2~0.5 part of aloe and 0.9
~1.2 parts of hawthorn.
3. fermentation composition as claimed in claim 1, it is characterised in that the functional oligose is selected from chitosan, low
Xylan, kanjak mannan-oligosaccharides, mannan-oligosaccharides, milk ketose, raffinose, soyabean oligosaccharides, FOS, oligomeric gala
One or more in sugar, lactosucrose, konjac mannan sugar, trehalose, palatinose and inulin;
The Bifidobacterium includes one kind in bifidobacterium longum, bifidobacterium breve, bifidobacterium infantis and bifidobacterium lactis
Or it is several;
The lactobacillus include Lactobacillus rhamnosus, Lactobacillus casei, lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus salivarius,
Lactobacillus helveticus, Bu Shi lactobacillus, lactobacillus bulgaricus, lactobacillus paracasei and one kind or several in lactobacillus reuteri
Kind.
4. fermentation composition as claimed in claim 1, it is characterised in that it is 8 that the fermentation composition, which also includes parts by weight,
~20 parts of resistant dextrin, 5~8 parts of phytosterol and 3~6 parts of γ-aminobutyric acid.
5. fermentation composition as claimed in claim 4, it is characterised in that the functional oligose is xylo-oligosaccharide;
The probiotics is probiotics powder agent, is uniformly mixed by the pulvis of following parts by weight:45~60 parts of Bifidobacterium,
20~25 parts of Lactobacillus rhamnosus, 12~16 parts of Lactobacillus casei, 14~18 parts of lactobacillus acidophilus and Lactobacillus plantarum 23~
30 parts, the viable bacteria content of the probiotics powder agent is 2 × 108~8 × 108cfu/g。
6. such as fermentation composition according to any one of claims 1 to 5, it is characterised in that the fermentation composition also includes
Parts by weight are 1~3 part of saccharomycete, 1~3 part of acetobacter and 1~5 part of lactic acid bacteria;
The preparation method of the fermentation composition also includes:
(1) softwood is made in the fermentation composition of the powdery of 1/2 amount, is well mixed with the lactic acid bacteria of the parts by weight laggard
Row extrusion makes ball, and capsule core is made;
(2) fermentation composition of the powdery of 1/2 amount is well mixed with the saccharomycete and acetobacter of the parts by weight, made
It is standby into softwood thing;
(3) obtained capsule core is placed in the coating solution prepared in advance, be coated;
(4) the round as a ball extruding in the softwood thing of step (2) to the coating capsule core after Cotton seeds, then be dried, produce pellet
Fermentation composition;
Wherein, the coating solution is by the O-phthalic for the acroleic acid resin No. II, 0.05-1 parts that parts by weight are 0.4-10 parts
Diethyl phthalate and 1-3 parts of castor oil add the aqueous dispersion that appropriate purified water is configured to.
7. a kind of preparation method of fermentation composition as claimed in claim 4, it is characterised in that methods described includes following step
Suddenly:
1) preparation of Chinese medical extract:
The root of kudzu vine and cassia seed of the parts by weight are crushed respectively, 60 mesh sieves are crossed, root of kudzu vine powder and Cassia Seed is made;
By root of kudzu vine powder, the matrimony vine of Cassia Seed and predetermined weight part, mulberries, sea-buckthorn, aloe, 8 times of weight of hawthorn water,
4h is kept at 80-90 DEG C of temperature, 40 DEG C, plus the alcohol reflux 4h that 2 times of weight concentrations are 75%, filtering is then cooled to, obtains
First filtrate;Add the water of 3 times of weight of filter residue, 2.5h kept at 80-90 DEG C of temperature, be then cooled to 30 DEG C, filtering, the
Two filtrates;Merging filtrate, is concentrated under reduced pressure, is freeze-dried, and low-temperature grinding is into the traditional Chinese medicine fine powder for being ground into 0.05-0.2mm;
2) by step 1) in traditional Chinese medicine fine powder mixed with emulsifying agent, water after carry out high speed shear with cutter, form grain diameter
Chinese medicine particulate less than 50 microns;
3) by the functional oligose of predetermined weight number, resistant dextrin, phytosterol, γ-aminobutyric acid and step 2) in
Chinese medicine particulate is uniformly mixed, and is sterilized by heat exchanger, and condition is 99 DEG C, 300s;
4) mixture is cooled to 40-45 DEG C after sterilizing, and the probiotics for adding scheduled volume is freeze-dried, and adding dry method after being well mixed makes
Grain machine granulation, is made the fermentation composition of powdery, sterile packing is produced.
8. it is applied to the plant enzyme of cardiovascular and cerebrovascular diseases conditioning and its routine servicing any one of a kind of claim 1-5
Preparation method, it is characterised in that the described method comprises the following steps:
(a) powdery fermentation composition is prepared
(b) mixed material:1kg plant material is cleaned, is put into the sugar of 0.3~0.6kg weight in ferment bucket, adds 5~6L
Pure water, be well mixed, at the same add weight be 20~50g the step of (a) prepare fermentation composition to ferment bucket in, envelope
Lid, lucifuge fermentation is carried out in fermentation temperature is 28~30 DEG C of environment;
(c) ferment:Fermentate in ferment bucket is carried out after stirring sooner or later once daily, 5~8d of fermentation, filtering produces plant
Thing ferment.
9. it is applied to the plant enzyme of cardiovascular and cerebrovascular diseases conditioning and its routine servicing any one of a kind of claim 1-5
Preparation method, it is characterised in that the described method comprises the following steps:
A) fermentation composition of powdery is prepared
B) preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight is added into plant material
10L pure water, is well mixed, and stands after 1~2d of extraction, carries out high-temperature sterilization, and filtering obtains plant extraction liquid;
C) preparation of plant enzyme stoste:Composite flora is inoculated with into plant extraction liquid, mixing is placed in closed environment, carries out lucifuge
Fermentation 1~2 month, obtains plant enzyme stoste;
Wherein inoculum concentration is 8~12%;Composite flora is saccharomyces cerevisiae, the newborn subspecies of Lactobacillus delbrueckii, Lactobacillus plantarum, gram
It is several in lactobacillus, lactobacillus acidophilus and acetobacter;
D) preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L water with
And plant material 1kg, fermentation composition prepared by 150ml plant enzyme stoste and 30g step a) is added, in closed environment
Middle to be fermented, fermentation temperature is 28~30 DEG C, is deflated 1~2 time daily, after 4~6d of fermentation, and filtering produces plant enzyme.
10. it is applied to the preparation method of the plant enzyme of cardiovascular and cerebrovascular diseases conditioning and its routine servicing described in a kind of claim 6,
Characterized in that, the described method comprises the following steps:
I the fermentation composition of pellet) is prepared
II) the preparation of plant extraction liquid:3kg plant material is cleaned, the sugar of addition 1kg weight is added into plant material
10L pure water, is well mixed, and stands after 1~2d of extraction, carries out high-temperature sterilization, and filtering obtains plant extraction liquid;
III) the preparation of plant enzyme:From more than 10L drum, addition native malt sugar 125g, vitamine C sodium 5g, 8L water
And plant extraction liquid 1kg, add 30g step II) prepare pellet fermentation composition, sent out in closed environment
Ferment;Fermentation temperature is 30~32 DEG C, is deflated 1~2 time daily;Ferment after 1~2d, insertion conduit is passed through air, and ventilate 2-4 daily
It is secondary, each 10-30min, and control fermentation temperature to be 35~37 DEG C;Filtered after 5~7d of fermentation, produce plant enzyme.
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