CN101336705B - Health food and its preparation method - Google Patents
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Abstract
The invention discloses a health food for reducing weight, reducing blood pressure, reducing blood-lipid and reducing blood sugar, and a preparation method thereof. The inventive health food comprises, based on the weight part, 5 to 40 parts of black fungus, 5 to 10 parts of konjak and 1 to 6 parts of tuckahoe. On the basis that the natural flavor, colour and nutrient content of black fungus are retained, the preparation combines the traditional dietary cure health preserving formulation with the modern biotechnology, and makes the best of the unique bidirectional adjusting technic in Chinese medicine. After people orally take the black fungus beverage and black fungus capsule prepared by the invention, the metabolite in the cardiovascular system and gastrointestinal tract system of the body can be cleaned up, the endocrine system can be adjusted, and the stable ability of the inner environment of body can be enhanced, and the nutritional and metabolic disease can be prevented. The black fungus beverage and black fungus capsule prepared by the invention have the special effect for the prevention and auxiliary treatment of the metabolic chronic disease such as insulin resistance and the like, especially for abdominal obesity.
Description
Technical field
The present invention relates to a kind of health food, relate in particular to a kind of main be the fat-reducing that has of active ingredient with the black fungus, hypotensive, reducing blood lipid, the health food of effect such as hypoglycemic the invention still further relates to the preparation method of this health food, belongs to field of health care food.
Background technology
Fat of a kind of whole body incretion metabolism disease; Not only influence figure and activity; And it is closely related with diseases such as hyperlipidemia, atherosclerotic, coronary heart disease, diabetes, gout, fatty liver, endocrinopathy, the low ventilation of alveolar syndrome, cholecystitis; Fat become the problem that medical circle and biological educational circles press for solution, traditional traditional Chinese medicine is with the obvious advantage in this respect.Obesity also is person in middle and old age's common disease and frequently-occurring disease, is an important symbol that insulin resistance occurs, also is one of definite difficult disease of the World Health Organization.
Abdomen type obesity is one of principal element of human body diabetes B, angiocardiopathy and metabolic syndrome generation.With metabolic syndrome (MS) is example: the U.S. in 1988-1994 for the third time national health and nutrition survey (NHNESIII) confirm that along with the growth at age, illness rate also rises rapidly, the illness rate in 60-69 year has reached 43.5%.Although the Chinese population bluntness is not as good as the west crowd, the illness rate of MS also reaches more than 14%, and Chinese population is more obvious with abdominal obesity.Investigation to cities such as Shanghai, Beijing, Qingdao shows that male sex MS illness rate is than women higher (man/woman: Shanghai 18.36%/15.82%, Beijing 30%/18%, Qingdao 16.1%/9.4%).
The human internal environment is constant to be that extracellular fluid has constant relatively physicochemical property, and keeping the relative constant levels of various physical signs such as body temperature, blood pressure, blood sugar, blood fat is to keep the necessary primary condition of normal activities.Interior environment disorder that the internal factor of the physics and chemistry of human body internal and external environment and biological factor (like the drastic change of temperature, ray, noise, high light, electric shock, low pressure, hypoxemia, poisoning, wound, infection etc.) and body (change of blood heat or composition, heart function are low, arrhythmia cordis, organ dysfunction disorder and property are constrained etc.) is caused or homeostasis are unbalance to be one of maximum harm of current health.Human body " homeostasis " and " cellular stress " are important breakthroughs in 20th century modern medicine study field; It will be the brand-new bright spot in 21 century health field, wherein the cells involved environment with stress research become one of focus of current life science.
Cellular environment is following with the main development course that stress study: nineteen twenty-nine, Connon at first proposes to describe body with homeostasis (homeostasis) speech and keeps its stable phenomenon.He finds that animal is when fistfight-reaction of flight (fight and fight response); Sympathin in the blood (confirming it is norepinephrine and adrenergic mixture later on) increases, and proposes stomodaeal nervous system in emergency circumstances plays important balanced action at body urgent theory (emergency theory).1936, Selye be the 1st and in medical domain, propose " stress " and the people of stressor notion.Selye has described a kind of pathology triad that can be caused by multiple stimulations such as bacterial infection, poisoning, X line, wounds (adrenomegaly, gastrointestinal ulceration, thymic lymphoma knot are degenerated) in the paper that the Nature magazine is delivered.He thinks that these variations are body a kind of adaptation reactions when suffering destructive stimulus; It is referred to as general adaptation syndrome (general adaption syndrome) or emergent syndrome (stress syndrome), and can causes that the stimulation of emergency reaction is called emergent former.The eighties in 20th century, Sterling and Eyer proposed unstable state (allostasis) speech, and it is defined as a kind of ability of keeping homeostasis through changing.McEwen in 1993 has been incorporated into unstable state stress research field, its unstable state load notion explained better stress and the relation of disease between taking place, developing.
" negative and positive " and " actual situation " are two key concepts of Chinese traditional traditional Chinese medicine.Modern human body " homeostasis " and " stress " notion and Chinese traditional traditional Chinese medical science in the theoretical system of " yin and yang in equilibrium " and " removing real qi-restoratives " have similitude." warp in the emperor. element is asked. the big opinion of YIN YANG classification of natural phenomena " point out " negative and positive person, the road in the world also, the general order and law of all matters, the root cause of various changes of the universe, the motive power of flourishing and perishing of matters, the house of mind are also." " negative and positive person, the men and women of vim and vigour are also "." the kind person of examining examines look and feels the pulse, distinguishing yin and yang at first "." warp in the emperor. element is asked. the negative and positive another matter " so the diagnosis outline that proposes the traditional Chinese medical science " not in positive person, know when disease is avoided,, know the phase of dead life not in cloudy person.Careful ripe negative and positive do not have and many schemes "." warp in the emperor. element is asked. the logical actual situation opinion of commenting " in point out " perverse trend Sheng person is real, consumption of normal qi causing asthenia ".Carry in " Huatuo's Zhongzang classic ": " its real person must declare logical property must prolong its longevity; Its empty person, the feelings that get qi-restoratives must be grown its year.”
Up to now, medically mainly lose weight through following several kinds of modes:
(1) adopt appetite inhibitor, like the fenfluramine right side etc., but because of its potential cardiovascular adverse effects, few at present using.
(2) metabolism Reinforcing agent is like thyroid hormone formulations, because of its cardiovascular side effects is progressively used less.
(3) thrombocytin and NRI, like sibutramine, but side effect is big, mainly shows as nauseating, dry, poor appetite, heartbeat, fast nervous constipation, insomnia.
(4) lipase suppresses, and like orlistat, major side effects is fatty liver, stench.
(5) operative treatment: liposuction, cut fat, jejunoileum bypass, little stomach operation and vertical ligation stomach urethroptasty, expense is bigger, and needs of patients bears certain risk and misery.
Though aforesaid way all has a certain effect for fat-reducing, not degree to exist side effect big, bounce-back easily, defectives such as medical expense height have much room for improvement.
Summary of the invention
Technical problem to be solved by this invention is the deficiency that overcomes prior art, and a kind of health food with definite effect of weight reducing is provided, and this health food has no side effect, also have hypotensive, reducing blood lipid, health-care efficacy such as hypoglycemic.
Technical problem to be solved by this invention realizes through following technical scheme:
A kind of health food with fat-reducing, hypotensive, reducing blood lipid, effect of lowering blood sugar comprises that each raw material of following weight portion is processed: black fungus 5-40 part, konjaku flour 5-10 part and Poria cocos 1-6 part;
Preferably, the weight portion of each raw material is: black fungus 20-30 part, konjaku flour 6-9 part and Poria cocos 2-4 part;
Preferred, the weight portion of each raw material is: 3 parts in 25 parts of black fungus, 8 parts of konjaku flours and Poria cocos;
In order to reach better effect, also can add the date of 4-20 weight portion in the raw material of above-mentioned health food, be preferably the date that adds 8 weight portions;
Further, also can add the Radix Glycyrrhizae of the ginger and the 1-5 weight portion of 1-6 weight portion in the raw material of above-mentioned health food, preferred, add the Radix Glycyrrhizae of the ginger and 2 weight portions of 3 weight portions.
Black fungus: Mycophyta Basidiomycetes Auriculariaceae Auricularia plant auricularia auriculajudae Auricularia auricula (L.) Underw., be used as medicine with fructification.Xia Qiu gathers, and dries.The black fungus delicate mouthfeel is clear and melodious, and is sliding tender good to eat, and delicious flavour is nutritious, protein abundance not only, and be rich in multivitamin and mineral matter, and particularly iron content is high, and per 100 gram dried fungus iron content reach 185 milligrams, are 100 times of meat.Useful gas, the strong intelligence of allaying one's hunger, make light of one's life by commiting suicide, hemostasis and pain-relieving, the effect such as invigorate blood circulation of enriching blood.Black fungus is rich in the polysaccharide colloid, and good clear sliding effect is arranged, and also has certain anticancer and treatment angiocardiopathy function.Colloid in the black fungus can put together and excretes remaining in dust, impurity absorption in the digestion, thereby plays the effect that intestines are washed in clearing stomach.Simultaneously; Its fiber substance function that helps digest in addition; Foreign matters such as the hair that is difficult to digest eaten unintentionally, husk, wooden slag, sand, metal fillings are had dissolving and molten effect, and therefore, it is the indispensable health food of mine, chemical industry and textile worker.Black fungus also has endogenous foreign matters such as gall stone, kidney stones dissolves function more significantly.Adenosine in the black fungus has the thrombotic effect of significant inhibition, can reduce blood clotting, and sick generations such as prevention thrombus have the effect of atherosclerotic of preventing and treating and coronary heart disease.
Poria cocos: property is sweet, light, equal, return lung, stomach, kidney channel, favourable aquation drink, the peaceful heart of invigorating the spleen, the effect of beneficial spleen and stomach.
Date: have another name called red date, dried jujube, Chinese date.Be rich in protein, fat, carbohydrate, carrotene, B family vitamin, vitamin C, citrin and nutritional labelings such as calcium, phosphorus, iron and CAMP.Wherein ascorbic content comes out at the top in fruit, and vitamin king's laudatory title is arranged.The CAMP that is contained is the essential composition of human body cell energetic supersession, can strengthen muscular strength, eliminates fatigue, hemangiectasis, increase myocardial contractive power, improve myocardial nutrition, to preventing and treating disease of cardiovascular system good effect is arranged.
The traditional Chinese medicine theory thinks, red date has effects such as qi-restoratives benefit gas, nourishing blood and tranquilization, strengthening the spleen and stomach, is patient's good health care nutriment such as weakness of the spleen and the stomach, insufficiency of vital energy and blood, lassitude, insomnia and dreamful sleep.Citrin content in the date is the hat of all fruits and vegetables, and it has the capillary permeability of keeping, thereby improves the effect of microcirculation prevention of arterial sclerosis, also can promote vitamin C in human body, to put aside.In addition, contained soap-like matter in the date has effects such as the body metabolism of adjusting, enhance immunity power, anti-inflammatory, antiallergic action, reduction blood sugar and cholesterol level; Contained rutin has protection capillary vascular patency, prevents the function that vascular wall fragility increases, and is sick effective in cure to hypertension, atherosclerotic etc.; Contained Flavonoid substances can be used for hypertension and arteriosclerotic treatment and prevention.Contain abundant vitamin C, citrin in the date, to perfecting capillary, keeping vessel wall elasticity, antiatherosclerosis is very useful; Contain cAMP in the date, its pharmacological action shows as and improves microcirculation in human body, and coronary artery dilator increases the amount of blood supply of brain and heart, decreasing heart rate, and reduction MCO and improve the metabolism of ischemic myocardium is so can prevent and treat cardiovascular and cerebrovascular diseases.
Konjaku: flavor is hot, cold in nature, has detoxifcation, detumescence, row what, reduces phlegm, diffusingly multiple function such as amasss.Main Ingredients and Appearance Portugal mannose is rich in food fiber, and several amino acids and trace element are often edible a lot of to the human body benefit.One of which, the maximum glucomannan of content has powerful expansive force in the konjaku, and the sticking toughness that surpasses any natural plant gum is arranged, and can fill stomach and intestine, eliminates hunger, because of institute's heat content is very little, so can control body weight, reaches the purpose of defatting beauty again; Its two, the effect of lipopenicillinase is arranged.Contain some chemical substances in the konjaku, can reduce serum cholesterol and triglycerides, effectively alleviating hypertension and angiocardiopathy; Its three, konjaku can make intestinal enzymes secretion increase, and accelerates to remove deposit on the intestines wall, and it is excreted as early as possible.The helping digestion so konjaku can whet the appetite can be removed enteron aisle rubbish again, has the control obesity, the effect of promoting longevity; Its four, konjaku also contains the favourable pectin of human body, alkaloid, 17 seed amino acids and trace element, also has tangible health-care efficacy for diabetes.
Ginger: be the fresh rhizome of ginger ginger Zingiber officinale Rosc., hot, the tepor of nature and flavor is the pungent stomachic tonic of armaticity, and effects such as warm, excited, sweating, preventing or arresting vomiting, detoxifcation are arranged.
Also can add an amount of flavouring, toner etc. in the above-mentioned health food, wherein said flavouring is preferably xylitol etc.
Xylitol is a kind of pentose alcohol, is the normal intermediate product of xylose metabolism, and profile is the crystallinity white powder; As a kind of functional sweetener; Can participate in body metabolism, get into blood after, do not need insulin just can penetrate cell and accretion rate is fast; Can not cause that blood sugar raises, and is best suited for the auxotype sugar substitute in patients with diabetes mellitus.
Used Radix Glycyrrhizae can also increase the fragrance of beverage among the present invention, improves its denseness and color and luster.
Another technical problem to be solved by this invention provides a kind of method for preparing above-mentioned health food.
Another technical problem to be solved by this invention realizes through following technical scheme:
A kind of preparation is above-mentioned to have a fat-reducing, and the method for the health food of hypotensive, reducing blood lipid, effect such as hypoglycemic comprises:
(1) takes by weighing each raw material by said weight portion;
(2) with using flooding after the abundant rehydration of black fungus, leaching liquor is filtered, the extract that gets the black fungus water-soluble substances is subsequent use; Black fungus water-insoluble materials after the lixiviate is mixed with konjaku flour, and granulation, can get capsule;
(3) Poria cocos, ginger, date and Radix Glycyrrhizae water are decocted, decoction liquor is filtered, and the extract of collecting the prepared black fungus water-soluble substances of filtrating and step (2) mixes, and adds flavouring, toner, is prepared into solid beverage after the vacuum drying; Or solid beverage allotment added water, sterilization, can, soft drink.
Preferably, the lixiviate described in the step (2) is the water that adds 80 times of weight in the black fungus after rehydration, under 80 ℃ temperature conditions, decocts 2 hours;
In the step (2) dried black fungus water-insoluble materials and konjaku flour are preferably mixed according to 1: 3 part by weight, granulate, can, capsule;
Decoction described in the step (3) preferably adds the water of 80-120 times of weight, boiled after, under 80 ℃ temperature conditions the insulation 30-120 minute.
Black fungus contains abundant cellulose and a kind of special plant colloid, can promote gastrointestinal peristalsis, impels the drainage of enteron aisle fat food, reduces the absorption of food fat; Poria cocos has the diuresis function, can increase electrolytical discharges such as potassium, sodium, chlorine in the urine (be used to treat various oedema, it has the remarkable result that strengthens insulin active external experiment proof); Both share the function with " opening pores, promoting diuresis to alleviate water retention ", can remove human internal environment's metabolin fast; Get rid of the body metabolism obstacle; The disorderly self-capability for correcting of the internal environment of enhances human body, supplemental treatment abdomen type is fat, effectively prevents the generation of nutrition and metabolism class disease; Having one subtracts three and falls function (slimming the abdomen hypotensive, reducing blood lipid, hypoglycemic).
In addition, konjaku also has stronger fat-reducing, hypotensive, reducing blood lipid, effect such as hypoglycemic; Contain abundant vitamin C, citrin in the date, to perfecting capillary, keeping vessel wall elasticity, antiatherosclerosis is highly profitable.
Health food of the present invention with traditional traditional Chinese medical science " The liver and the kidney have a common source " theoretical with " homeostasis " with " cellular stress ' ' wait the modern medicine study achievement to combine; be main component with the black fungus; auxiliaryly form; reach " Yin Yang balancing " purpose through " removing real qi-restoratives ", effectively clear up the adjusting of human body metabolin and internal system, the fast and stable ability of environment in the enhances human body; the generation of prevention nutrition and metabolism class disease; be the functional food of a kind of prevention of metabolic syndrome (MS) and supplemental treatment abdomen type obesity with medicinal fungi Poria cocos and medicinal and edible plant date, ginger and Radix Glycyrrhizae, prevent and metabolism class chronic disease such as supplemental treatment insulin resistance aspect have uniqueness effect, especially obvious to abdomen type obesity effect.
Experimental result and pharmacology analysis conclusion prove that all health food of the present invention is the effective means of supplemental treatment and prevention of obesity; Have clean internal organs, push away the good result of Chen Zhixin; Malnutritive and overnutrition are had the bidirectional modulation function, solve problem of obesity from the angle of getting rid of dysbolism.
Health food mouthfeel of the present invention is good, and unique flavor can be all kinds of consumer groups and likes or accept, and is nutritious, has no side effect, and can eat for a long time.
The specific embodiment
Further describe the present invention below in conjunction with specific embodiment, advantage of the present invention and characteristics will be more clear along with description.But these embodiment only are exemplary, scope of the present invention are not constituted any restriction.It will be understood by those skilled in the art that and down can make amendment with form or replace without departing from the spirit and scope of the present invention, but these modifications and replacing all fall in protection scope of the present invention the details of technical scheme of the present invention.
Embodiment 1
(1) takes by weighing each raw material (unit: kg): black fungus 25, Poria cocos 3, konjaku flour 8 by following weight;
(2) black fungus is selected impurity elimination, clean up, fully insert in the extractor after the rehydration, add water by 1: 80 part by weight and extract, extract temperature and be controlled to be 80 ℃, extraction time is 180 minutes; Leaching liquor is filtered, and it is subsequent use to get the black fungus extract; Black fungus water-insoluble materials after the lixiviate is placed on place mat and has vacuum drying on the pallet of konjaku flour, mix, granulate, can, process capsule;
(3) in Poria cocos, add the water of 100 times of weight, boiled after, under 80 ℃ temperature conditions, be incubated 90 minutes; Decoction liquor is filtered, and collects filtrating and mixes with the prepared black fungus extract of step (2), adds an amount of xylitol, and vacuum drying gets solid beverage; Perhaps the solid beverage allotment is added water, sterilization, oral liquid is processed in can.
Embodiment 2
(1) takes by weighing each raw material (unit: kg): black fungus 5, Poria cocos 1, konjaku flour 5 by following weight;
(2) black fungus is selected impurity elimination, clean up, fully insert in the extractor after the rehydration, add water by 1: 80 part by weight and extract, extract temperature and be controlled to be 80 ℃, extraction time is 150 minutes; Leaching liquor is filtered, and it is subsequent use to get the black fungus extract; Black fungus water-insoluble materials after the lixiviate is placed on place mat and has vacuum drying on the pallet of konjaku flour, mix, granulate, can, capsule;
(3) in Poria cocos, add the water of 80 times of weight, boiled after, under 80 ℃ temperature conditions, be incubated 90 minutes; Decoction liquor is filtered, and collects filtrating and mixes with the prepared black fungus extract of step (2), adds xylitol and an amount of flavouring, toner, and vacuum drying gets solid beverage; Perhaps the solid beverage allotment is added water, sterilization, soft drink is processed in can.
Embodiment 3
(1) takes by weighing each raw material (unit: kg): black fungus 40, Poria cocos 6, konjaku flour 10 by following weight;
(2) black fungus is selected impurity elimination, clean up, fully insert in the extractor after the rehydration, add water by 1: 80 part by weight and extract, extract temperature and be controlled to be 80 ℃, extraction time is 180 minutes; Leaching liquor is filtered, and it is subsequent use to get the black fungus extract; Black fungus water-insoluble materials after the lixiviate is placed on place mat and has vacuum drying on the pallet of konjaku flour, mix, granulate, can, capsule;
(3) in Poria cocos, add the water of 100 times of weight, boiled after, under 80 ℃ temperature conditions, be incubated 90 minutes; Decoction liquor is filtered, and collects filtrating and mixes with the prepared black fungus extract of step (2), adds xylitol and an amount of flavouring, toner, and vacuum drying gets solid beverage; Perhaps the solid beverage allotment is added water, sterilization, soft drink is processed in can.
Embodiment 4
(1) takes by weighing each raw material (unit: kg): black fungus 25, Poria cocos 3, konjaku flour 8, ginger 3, date 8, Radix Glycyrrhizae 2 by following weight;
(2) black fungus is selected impurity elimination, clean up, fully insert in the extractor after the rehydration, add water by 1: 80 part by weight and extract, extract temperature and be controlled to be 80 ℃, extraction time is 180 minutes; Leaching liquor is filtered, and it is subsequent use to get the black fungus extract; Black fungus water-insoluble materials after the lixiviate is placed on place mat and has vacuum drying on the pallet of konjaku flour, mix, granulate, can, capsule;
(3) in Poria cocos, ginger, date and Radix Glycyrrhizae, add the water of 100 times of weight, boiled after, under 80 ℃ temperature conditions, be incubated 60-90 minute; Decoction liquor is filtered, and collects filtrating and mixes with the prepared black fungus extract of step (2), adds xylitol and an amount of flavouring, toner, and vacuum drying gets solid beverage; Perhaps the solid beverage allotment is added water, sterilization, can gets soft drink.
Embodiment 5
(1) takes by weighing each raw material (unit: kg): black fungus 5, Poria cocos 1, konjaku flour 5, ginger 1, date 4, Radix Glycyrrhizae 1 by following weight;
(2) black fungus is selected impurity elimination, clean up, fully insert in the extractor after the rehydration, add water by 1: 80 part by weight and extract, extract temperature and be controlled to be 80 ℃, extraction time is 180 minutes; Leaching liquor is filtered, and it is subsequent use to get the black fungus extract; Black fungus water-insoluble materials after the lixiviate is placed on place mat and has vacuum drying on the pallet of konjaku flour, mix, granulate, can, capsule;
(3) in Poria cocos, ginger, date and Radix Glycyrrhizae, add the water of 100 times of weight, boiled after, under 80 ℃ temperature conditions, be incubated 90 minutes; Decoction liquor is filtered, and collects filtrating and mixes with the prepared black fungus extract of step (2), adds xylitol and an amount of flavouring, toner, and vacuum drying gets solid beverage; Perhaps the solid beverage allotment is added water, sterilization, can gets soft drink.
Embodiment 6
(1) takes by weighing each raw material (unit: kg): black fungus 40, Poria cocos 6, konjaku flour 10, ginger 6, date 20 by following weight;
(2) black fungus is selected impurity elimination, clean up the abundant rehydration in back and insert in the extractor, add water by 1: 80 part by weight and extract, extract temperature and be controlled to be 80 ℃, extraction time is 180 minutes; Leaching liquor is filtered, and it is subsequent use to get the black fungus extract; Black fungus water-insoluble materials after the lixiviate is placed on place mat and has vacuum drying on the pallet of konjaku flour, mix, granulate, can, capsule;
(3) in Poria cocos, ginger and date, add the water of 100 times of weight, boiled after, under 80 ℃ temperature conditions, be incubated 90 minutes; Decoction liquor is filtered, and collects filtrating and mixes with the prepared black fungus extract of step (2), adds xylitol and an amount of flavouring, toner, and vacuum drying gets solid beverage; Perhaps the solid beverage allotment is added water, sterilization, can gets soft drink.
The effect of weight reducing test of Test Example 1 health food of the present invention
1, materials and methods
1.1. capsule that (1) given the test agent: embodiment of the invention 1-3 is prepared and beverage; (2) control sample 1: black fungus extract (preparing according to the disclosed method of embodiment of the invention 1-3); (3) control sample 2: the Poria cocos water extracts resulting extract (method according to embodiment of the invention 1-3 prepares);
1.2. animal used as test: the Wister big white mouse that provides by institute of oncology, Heilongjiang Province Experimental Animal Center (approval number is the moving word 09-0-33 of doctor), 130 of the second day healthy white mouse of being born are divided into 13 groups at random; Every group 10; Wherein one group as control group (normal raise), all the other 120 after raising modeling in 38 days, be divided into 12 groups at random; Every group 10; One group as observation group (not giving any slimming medicine or food treats), 9 groups of capsule and beverages (being divided into high, medium and low three dosage respectively) that the embodiment 1-3 that feeds respectively is prepared wherein, remaining two group feed respectively control sample 1 and control sample 2 (dosage is identical with given the test agent).
1.3 dosage design: the human body RD is everyone every mouthful 4g, as becoming body weight for humans in 60 kilograms, then is equivalent to 660mg/ day/kg body weight.The dose,equivalent of mouse is 1 times of human body RD, i.e. 660mg/kg body weight every day (low dosage), on, respectively establish a dose groups 1980mg/kg body weight (high dose) and 330mg/kg body weight (low dosage) down.
1.4 test apparatus and reagent:
Light microscope, Japanese Olympus; 730 type semi-automatic biochemical analyzers, day island proper Tianjin;
Triglyceride (TG), T-CHOL (TC), HDL (HDL_C), LCA (LACT) detection kit, Nanjing is built up bio-engineering research and is produced; Leptin (Leptin) detection kit, Linco produces.
1.5 experimental technique:
1.5.1 experimental obese rat model preparation: newborn male white rat, from birth second day.Every hypodermic injection paddy amino acid (MSG) normal saline solution, continuous 6 days, from birth the last the 22nd day, irritated stomach by body weight and give fat breast (10% cholesterol, 20% lard, 1% methylthiouracil, 2% sodium taurocholate) 10ml/kg, continuous 10 days every day.Be divided into 9 groups by lipschitz exponent (Lee Sindex) stratified random subsequently, give the given the test agent of various dose respectively, continuous 28 days, continue simultaneously to give and the fat breast.Fasting 24h after the last administration, inspection weighs sb., body is long, etherization subsequently, the arteria carotis communis intubate is got blood, separation of serum.
1.5.2 morphological observation is according to above-mentioned body weight, body is long, calculates lipschitz exponent; It is all fatty on every side to get animal testis, weighs; Get fritter fat after weighing, conventional fixing, section, 400 power microscopes are the number of cells in the full visual field of counting down.
1.5.3 blood fat, HDL_C and LACT measure, it is active to measure serum TC, TG, HDL_C content and LACT by kit method (being enzyme process).
1.5.4 Serum Leptin Levels (Lcptin) level determination is measured Serum Leptin Levels (Lcptin) level by kit method (putting the method for exempting from).
1.5.5 each group of statistics respectively with control group row T-TEST program in SAS.
2, result of the test
2.1. the fat intervention effect result of the test of animal is shown that given the test agent truly has the obvious suppression effect to the experimental obesity due to MSG and the fatty breast; Just can suppress rat body weight in the given the test agent during dosage increases; Suppress all fat weights of testis and adipocyte and increase, can obviously reduce lipschitz exponent.Given the test agent will obviously be superior to control sample 1 and control sample 2 to the general effect of the fat intervention of animal.
2.2 influence to thrombocytin (leptin) level
Dosage, high dose all can reduce the thrombocytin level of the experimental obese rat due to MSG and the fatty breast in the result of the test demonstration given the test agent, conform to its influence to lipschitz exponent, and control sample 1 does not have obviously influence with control sample 2 to it.
2.3. to serum TG, TC, the influence of HDL_C content and LACT
The blood fat (TG/TC) that dosage, high dose all can reduce the high fat of blood animal in the given the test agent increases HDL_C content, and the show dose dependence.Control sample 1 has certain effect for reducing fat with control sample 2, but its effect will obviously be weaker than given the test agent, and HDL_C is not had obvious influence.
Can find out that from result of the test the body weight that given the test agent can significantly suppress experimental obese rat increases, and reduces lipschitz exponent; Suppress testis week fat pad weight, adipocyte volume; Show significant effect of weight reducing, simultaneously, the blood fat of animal, the variation of lipoprotein are corresponding basically with the intensity of fat-reducing; Its whole effect of weight reducing will obviously be superior to the effect of weight reducing of control sample 1 and control sample 2
The safety testing of Test Example 2 health foods of the present invention
1 material and method
Capsule that 1.1 given the test agent: embodiment of the invention 1-6 is prepared and beverage.
1.2 experimental animal: Kunming kind small white mouse (approval card number, the doctor is moving to be learned 09-2-1 number), (the approval card number is Wister strain big white mouse: the moving 09-3-2 of doctor) available from institute of oncology, Heilongjiang Province Experimental Animal Center.
1.3 large and small white mouse per os acute toxicity test: select 40 of Kunming kind small white mouses for use, male and female half and half, body weight 20.0-22.0g is divided into 4 groups at random by sex, body weight, 5 every group.4 of Wister rats, male and female half and half, body weight 180-220g is divided into 4 groups at random by sex, body weight, 5 every group.
Carry out the dosage design by horn method, 4 dosage are respectively: 1.0g/kg body weight, 2.15g/kg body weight, 4.64g/kg body weight, 10.0g/kg body weight (this dosage is equivalent to 150 times of referrer's consumption).Sample is processed even suspension by set dosage with distilled water, and is subsequent use.
Behind the large and small mouse fasting 16h, press animal body and weigh 1 filling of 2% (every 100g irritates stomach 2ml) volume per os stomach, the poisoning manifestations and the death condition of observing large and small mouse, be 14 days observing time.
1.4 genetic toxicity test
1.4.1Ames test TA
97TA
98TA
100And TA
1024 bacterial strains provide by California, USA university Ames Lab, and sample is made into 5mg/0.1ml, 15mg/0.1ml, 0.2mg/0.1ml, 0.04mg/0.1ml, 0.008mg/0.1ml totally 5 concentration with DMSO.Except that above-mentioned 5 concentration group, be provided with again from beaming back and become contrast and sex-limited contrast (adds S
9With 1-AF (TA
102With 1,8-dihydroxy quinone), do not add S
9Use DEXON, adopt flat board to mix the method test, every ware addition is 0.1m.Each dosage is made 3 wares, and repeats once, reports twice result of the test at last with 3 ware mean value fenbie.
1.4.2 PCEMNR micronucleus test: Kunming kind small white mouse, body weight 25-30g, totally 50; Male and female half and half are established 3 dose groups, and the dosage design considerations is: manufacturer's recommended dosage is 4g people/day; Become body weight for humans in 60.0kg; Then be the 0.066g/kg body weight, high dose is made as 6.7g/kg body weight (being equivalent to recommend population 50 times with dosage), and low dose group is 1.7g/kg body weight (is equivalent to the daily dosage of referrer 25 times).Each group is all irritated stomach by the 20ml/kg body weight, and wherein positive controls per os filling stomach dosage is the endoxan of 60.0mg/kg body weight; Negative control group adopts interval 24h to give the thing method of being tried twice with isopyknic clean water per os filling stomach, promptly for the first time to after being tried thing 24h, gives once more and is tried thing.Mouse is put to death in the cervical vertebra dislocation behind the 6h, gets the conventional film-making of femur.Every mouse of microscopically is observed 1000 polychromatic erythrocytes (PCE), records the cell number of micronucleus, calculates the micronucleus cell rate.
1.4.3 the mouse sperm deformity test: select 25 of Kunming kind male white mouses for use, body weight 25.0-30.0g is divided into 5 groups at random; Every group 5, establish 3 dose groups, dosage designs with the mouse bone marrow cells micronucleus test; Be that high dose is 6.7g/kg body weight (is equivalent to the daily dosage of referrer 50 times), low dose group is that 1.7g/kg body weight (be equivalent to the daily dosage of referrer 25 times) negative control group is given and distilled water, and positive controls is given and endoxan; Dosage is the 60mg/kg body weight, and all each groups are all irritated stomach by the 20ml/kg body weight, once a day; Continuous 5 days, each treated animal all in throw first with sample after put to death in 35 days, by the fixing 2% Yihong solution-dyed of conventional method film-making; 1000 complete sperms of every mouse microscopy calculate rate of teratosperm.
1.5 feeding trial
80 of the wi star rats of ablactation, body weight 60-80g is divided into 4 groups at random, and 20 every group, male and female are half the, and wherein three groups is test group, and one group is control group.
The same micronucleus test of sample treatment, dosage designs as follows: high dose is the 6.7g/kg body weight, is equivalent to recommend 100 times of consumption; Middle dosage is the 3.3g/kg body weight; Be equivalent to the people and recommend 50 times of consumption, low dosage is the 1.7g/kg body weight, is equivalent to the people and recommends 25 times of consumption.Irritate the stomach volume and undertaken by the 20ml/kg body weight, negative control is irritated isometric clean water, irritates stomach every day once, continuous irrigation stomach 30 days.
Observe and write down general situation, poisoning symptom and the death condition of animal every day.Claim body weight weekly one time, write down total food ration of every treated animal, and, calculate food utilization according to weightening finish in 30 days and food ration.After experiment finished, blood sampling detected hematological indices, and detected biochemical indicator, like glutamic-pyruvic transaminase (ALT), and glutamic-oxalacetic transaminease (AST), total protein (TP), albumin (ALB), urea (BUN), blood sugar (GLU), triglyceride (TG), cholesterol (TC) and courage glycosides.Cut rat abdomen simultaneously open, taking-up liver,kidney,spleen, testis, ovary are weighed, and calculate dirty body ratio, and carry out pathological examination.
1.6 traditional teratogenicity test
The Wistar rat, body weight 220-250g, the male and female rat is pressed 2: 1 with behind the cage previous night, early does the rat vagina plate coating checking next day; Be designated as the fertilization mouse to find sperm, and with the same day as " become pregnant " and the 0th day, find 60 of the mouse of being fertilized altogether; Be divided into 4 groups at random, 15 every group, wherein 3 groups is test group; 1 group of negative control group, per os is irritated stomach, and the time is gestation the 7th day to the 16th day.
Sample treatment and dosage design were with 30 days feeding trials.High dose group is the 6.7g/kg body weight, is equivalent to 100 times of people's RD; Middle dose groups is the 3.3g/kg body weight, is equivalent to 50 times of people's RD; Low dose group is the 1.7g/kg body weight, is equivalent to 25 times of people's RD, and irritating the stomach volume is the 20ml/kg body weight.Negative control group is given isometric(al) distilled water.
Become pregnant the 0th, 7,12,, claimed female mouse body weight in 16,20 days, observe the body weight change conditions, in the 20th day sacrificed by decapitation animal of becoming pregnant, laparotomy inspection at once, the young litter weight of weighing tire, the record tire of living, stillborn foetus and absorb the tire number.The outward appearance of inspection tire alive has or not deformity one by one then, does young growth of tire and measures, and 1/2 usefulness BOUINShi of the tire of living is liquid-solid fixed, does internal organ inspection fully; Other 1/2 through red S dyeing, does the bone stained preparation, carries out inspections such as skeleton development and deformity.
1.7 select for use the SYSTAT statistical software to carry out Poisson distribution, the square statistical analysis such as analytical control and mean t check of raising to test data according to handling.
2 result of the tests
2.1 optimum toxicity test
In 14 days viewing duration, it is good to be tried big mouse general state, poisoning symptom all do not occur, and dead the generation also do not arranged.According to the optimum toxicity criterion of dosage, observed result and large and small mouse that designed of test, can think the true border of given the test agent innocuous substance.
2.2 genetic toxicity test
2.2.1AMES test
Result of the test shows, receives the sample article adding and do not adding under S (hepatomicrosome) activation condition, and to the Salmonella reversion test result that all is negative, promptly the Salmonella reversion test result is negative for mutagenesis.
2.2.2 mouse marrow polychromatic erythrocyte micronucleus test
Result of the test is carried out Poisson distribution check, and two sex mouse of result are except that positive controls, and each dose groups is compared with the negative control group micronuclear rates, and equal no significant differences explains that the mouse bone marrow cells of being tried thing has a liking for the negative result of polychromatophilia micronucleus test.
2.2.3 mouse sperm deformity test
Each organizes the defective sperm that mouse all can be seen some; But the positive controls rate of teratosperm that gives endoxan is compared with negative control group; Utmost point significant difference is arranged; And the rate of teratosperm of each test group mouse is compared with negative control group, and there are no significant (P＞0.05), explains that being tried thing does not have the teratospermia incidence and influence.
2.330 it feeding trial
(1) in entire test, each treated animal generally in order, ' low dose group respectively has a unexpected death, and ((other each treated animals are not all seen has any tangible bad projection, more not having the generation that causes death of poisoning to irritate the stomach operate miss in only female.
(2) (P＜0.05=, the average weight of all the other each treated animals is in each phase difference (variance analysis, P＞0.05) of comparing with negative control group that there are no significant except that first and second all average weight of high dose group jenny significantly is lower than control group.
(3) to the influence of rat total foodstuff utilization rate
In the weight gain of high dose group buck and food utilization are starkly lower than the dose groups; The food utilization of each dose groups and control group there was no significant difference; Food-intake and the weight gain of also seeing the high dose group jenny in addition significantly are lower than control group, and the courage food utilization is respectively organized indifference.
(4) hematological examination result
Each dose groups animal total white blood cells, leukocyte differential count and content of hemoglobin are all in normal range (NR), and individual dose groups compares no conspicuousness difference with control group, explain that the above-mentioned hematological indices of rat is not had obviously by the sample article influences.
(5) biochemical investigation in latter stage result
Put to death animal after the off-test; Isolated from rat serum has carried out the detection of the indexs such as activity, total protein, albumin ' urea nitrogen ' serum, triglyceride, cholesterol and inosine content of serum glutamic oxalacetic transaminase, Alanine transaminase, and result of the test shows; Each item index is all in range of normal value; And compare with negative control group, there was no significant difference explains that the above-mentioned blood biochemistry index of rat is not had obviously by the sample article influences.
(6) to the influence of the dirty bodyization of rat
Result of the test is carried out variance analysis, and the result shows, the dirty body there was no significant difference of each treated animal (P＞0.05).Pharmacology histological examination, each organizes the histopathologic examination of rat main organs, and all not seeing has the sick different Histological change different with control group, explains that given the test agent is to harmless effects of main organs such as rats'liver, spleen, kidney, testis.
2.4 traditional teratogenicity test
2.4.1 influence to pregnant mouse body weight
Each treated animal ide total augment weight is carried out statistical analysis, and each dose groups is compared with negative control, and there was no significant difference explains that given the test agent does not have obvious influence to pregnant mouse body weight.
2.4.2 to the general influence of growing of tire mouse
Result of the test shows that the absorption tire of each treated animal, stillborn foetus and average litter size are compared no statistics difference (P＞0.05) with negative control group; The young average weight of tire; Except that high dose group is starkly lower than other each groups, (variance analysis, Q check; P＜0.01); All the other each group is compared with negative control group, and no conspicuousness difference possibly be to be caused due to the tire mouse subalimentation owing to high dose group parent Excessive Intake tries thing (recommend consumption for the people 100 times).
2.4.3 influence to tire mouse development of bone marrow
The skeletal survey result finds that the slow index of each dose groups tire mouse skeleton development of given the test agent is compared with negative control group, there was no significant difference (X2 check, P＞0.05). in addition, each dose groups is sees skeleton deformity.
2.4.4 influence to tire mouse internal organ
Each dose groups of given the test agent is not all seen the tire mouse of outward appearance deformity, and internal organ are not also seen in internal organ free-hand section inspection has abnormal change.
The true border of given the test agent per os acute toxicity innocuous substance, AMES test mice marrow polychromatic erythrocyte micronucleus test, three genetic toxicity tests such as mouse sperm acute experiment detect and are negative findings; 30 days feeding trials also do not see that it has any toxicity damage effect to animal subject, and traditional teratogenicity test detects does not see that it is to tire mouse teratogenic effect or other tire sodoku property effects.
Claims (7)
1. health food, it is a capsule, comprises black fungus 20-30 weight portion and konjaku flour 6-9 weight portion, its preparation method takes by weighing each raw material for (1) by said weight portion; (2) with using flooding after the abundant rehydration of black fungus, leaching liquor is filtered, the extract that gets the black fungus water-soluble substances is subsequent use; Black fungus water-insoluble materials after the lixiviate is mixed with konjaku flour, and granulation, can get capsule.
2. health food, it is solid beverage or soft drink, comprises black fungus 20-30 weight portion, Poria cocos 2-4 weight portion, ginger 1-6 weight portion, date 4-20 weight portion, Radix Glycyrrhizae 1-5 weight portion, its preparation method takes by weighing each raw material for (1) by said weight portion; (2) with using flooding after the abundant rehydration of black fungus, leaching liquor is filtered, the extract that gets the black fungus water-soluble substances is subsequent use; (3) Poria cocos, ginger, date and Radix Glycyrrhizae water are decocted, decoction liquor is filtered, and the extract of collecting the prepared black fungus water-soluble substances of filtrating and step (2) mixes, and adds flavouring, toner, is prepared into solid beverage after the vacuum drying; Or solid beverage allotment added water, sterilization, can, soft drink.
3. according to the health food of claim 1, it is characterized in that the weight portion of each raw material is: 25 parts of black fungus, 8 parts of konjaku flours.
4. according to the health food of claim 2, it is characterized in that the weight portion of each raw material is: 25 parts of black fungus, 3 parts in Poria cocos, 3 parts in ginger, 8 parts in date, 2 parts in Radix Glycyrrhizae.
5. according to claim 1 or 2 described health foods, it is characterized in that: the lixiviate described in the step (2) is the water that adds 80 times of weight in the black fungus after rehydration, under 80 ℃ temperature conditions, decocts 2 hours.
6. according to the described health food of claim 1, it is characterized in that: in the step (2) dried black fungus water-insoluble materials is mixed with the part by weight of konjaku flour according to 1: 3, granulation, can get capsule.
7. according to the described health food of claim 2, it is characterized in that: the decoction described in the step (3) is: add the water of 80-120 times of weight, with water boiled after, under 80 ℃ temperature conditions, be incubated 30-120 minute.
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| CN106578754A (en) * | 2016-11-18 | 2017-04-26 | 安徽禾泉农庄生态农业有限公司 | Black fungus slimming beverage |
| CN109010694A (en) * | 2018-09-21 | 2018-12-18 | 武汉万密斋养生堂科技发展股份有限公司 | A kind of konjaku with function of blood sugar reduction, Pachymaran composition and preparation method thereof |
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| CN1593557A (en) * | 2004-07-15 | 2005-03-16 | 李洋 | Stomach intestine clearing capsule |
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Non-Patent Citations (4)
| Title |
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| 吕圭源等.降血压中药概述.《降压减脂中药》.上海科学技术出版社,2002,(第1版),第14-15页. * |
| 曲鸣等.生甘草与炙甘草各有何功用.《中医学难解——中药分册》.天津科学技术出版社,1987,(第1版),第96-97页. * |
| 杜红霞等.辅助降糖保健食品的研究现状.《中国食物与营养》.2006,(第9期),第18-21页. * |
| 陈锡强等.具有减肥作用中药的研究进展.《陕西中医学院学报》.2003,第26卷(第6期),第62-64页. * |
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