CN102614499B - Yak skin collagen compound traditional Tibetan medicine with anti-fatigue effect and preparation process thereof - Google Patents

Abstract

The invention relates to a yak skin glue compound Tibetan medicine with anti-fatigue effect. The compound Tibetan medicine is made of the following raw materials in parts by weight: 20-50 parts of yak skin collagen powder, 20-70 parts of Rhodiola rosea, and 5 parts of astragalus ~15 parts, ginseng fruit 10~50 parts, wolfberry 5~40 parts. At the same time, the invention also discloses a preparation process of the modified Tibetan medicine. In the present invention, the yak skin collagen powder is reasonably compatible with rhodiola rosea, astragalus, ginseng fruit, and wolfberry, and the obtained product has anti-fatigue effects. At the same time, the development of yak skin collagen enables the unique resource yak on the Qinghai-Tibet Plateau to obtain a deeper level of value reflect.

Description

A kind of yak skin glue compound Tibetan medicine with anti-fatigue effect and its preparation process

technical field

The invention relates to the technical field of Tibetan medicine composition and its preparation, in particular to a yak skin glue compound Tibetan medicine with anti-fatigue effect and its preparation process.

Background technique

In today's increasingly fierce competition, people's work and life rhythm are constantly accelerating, and modern people can easily enter a state of fatigue. Due to long-term fatigue without improvement, the body is always in a sub-health state, which is also the main reason for the frequent occurrence of "sudden death" in modern society.

Fatigue can be divided into physical fatigue and psychological fatigue. Physiological fatigue is the physical response of fatigue, and psychological fatigue is the psychological response of fatigue. It is generally believed that the physiological changes of fatigue are: the accumulation of lactic acid and other metabolites, the decrease of muscle tension, and the decrease of exercise durability. Psychologically, it is manifested as: lack of energy, malaise, memory loss, unresponsiveness, anxiety or fear, irritability or depression, suspicion or boredom, excessive psychological pressure, etc. Studies in recent years have shown that physical fatigue is often closely related to the energy metabolism of the body, while psychological fatigue is not only caused by physical fatigue, but also related to the adaptive adjustment of the body by the central nervous system in response to bad emotions. Although the recovery mechanisms of the two are not completely the same, but It is an indivisible whole. Therefore, the development of anti-fatigue products should not only pay attention to the recovery of physiological functions, but also carry out the overall conditioning and recovery of the body.

In view of the current anti-fatigue products only aiming at the recovery of physiological functions, and ignoring the restoration of the whole body while the recovery of physiological functions, the present invention develops the yak skin glue compound product according to the principle of Tibetan medicine prescription.

Contents of the invention

The technical problem to be solved by the present invention is to provide a yak skin glue compound Tibetan medicine with anti-fatigue effect, which takes yak skin collagen as the main component.

Another technical problem to be solved by the present invention is to provide a preparation process of the compound Tibetan medicine.

In order to solve the above problems, a yak skin glue compound Tibetan medicine with anti-fatigue effect according to the present invention is characterized in that: the compound Tibetan medicine is made of the following raw materials in parts by weight: 20 to 50 parts of yak skin collagen powder , 20-70 parts of rhodiola, 5-15 parts of astragalus, 10-50 parts of ginseng fruit, and 5-40 parts of wolfberry.

Described yak hide collagen protein powder is to make as follows:

① Clean the fresh or pickled yak skin of Wild Yak , a unique cattle species in the Qinghai-Tibet Plateau, and add an alkaline solution 3 to 10 times its weight in the yak skin with a concentration of 0.1 to 2 mol·L ^-1 Treat for 10-24 hours to obtain the depilated yak hide;

② Add 3 to 10 times the weight of water to the depilated yak skin to clean and soak in sequence, the number of soaks is 4 to 10 times, and each soak is 2 to 12 hours to remove the residual alkali; when the soaking solution When the pH value is 5~9, the dealkalized yak hide can be obtained;

③ Cut the dealkalized yak hide into small pieces with a length of 10 cm and a width of 5 cm, add water equivalent to 5 to 20 times its weight in grams, and extract by boiling at a temperature of 90 to 100 ° C for 2 to 6 hours Afterwards, supernatant A and filter residue A are obtained by filtration;

④ Add water equivalent to 5 to 20 times the weight of the filter residue A in milliliters, boil and extract at a temperature of 90 to 100°C, extract 2 to 10 times, and extract for 1 to 6 hours each time ; After filtering, the combined supernatant B and filter residue B are obtained; the filter residue B is discarded;

⑤ The combined supernatant B is spray-dried, pulverized, and passed through a 60-100 mesh sieve to obtain yak skin collagen powder.

The alkaline solution in the step 1. refers to any one of sodium hydroxide solution, potassium hydroxide solution, calcium hydroxide solution and barium hydroxide solution.

The above step ⑤ is replaced by the following method: the combined supernatant B is heated and concentrated at a temperature of 90~100°C to 1/30~1/10 of its total volume to obtain a concentrated solution; Boiling water bath at a temperature of 100°C until it becomes a viscous paste. The viscous paste is dried at a constant temperature at a temperature of 50-90°C to a constant weight, then pulverized, and passed through a 60-100 mesh sieve to obtain yak skin collagen protein powder.

The Rhodiola rosea is the dry root and rhizome of Rhodiola crenulata (Hook.f.et Thoms.) H.Ohba, a sedum plant.

The Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao or Astragalus membranaceus (Fisch.) Bge.

The ginseng fruit is the air-dried root tuber of Potentilla anserina Linn. var.

The wolfberry is the dry and mature fruit of Lycium barbarum L. of Solanaceae Ningxia wolfberry.

A kind of preparation technology of the above-mentioned yak skin glue compound Tibetan medicine with anti-fatigue effect, comprises the following steps:

⑴Preparation of API concentrate:

① Weigh the following raw materials by weight: 20-70 parts of rhodiola, 5-15 parts of astragalus, 10-50 parts of ginseng fruit, and 5-40 parts of wolfberry;

② After mixing the raw materials in step ①, add 5-20 times the total weight of the raw materials in milliliters of water, boil and extract at 90-100°C for 1-6 hours, and obtain the supernatant by filtration Liquid C and filter residue C;

③ Add 5-20 times the weight of water in milliliters to the filter residue C, boil and extract at a temperature of 90-100°C, the number of extractions is 2-10 times, and the extraction time is 1-6 hours each time; after filtering, the obtained Merge the supernatant D and the filter residue D; the filter residue D is discarded;

④ Heating and concentrating the combined supernatant D at a temperature of 90-100°C to 1/60-1/20 of its total volume, to obtain the concentrated liquid of the bulk drug;

(2) Add 20-50 parts by weight of yak skin collagen powder to the bulk drug concentrate obtained in the step (1), heat and dissolve at a temperature of 50-70° C., and stir evenly to obtain a compound semi-finished product of yak skin glue;

(3) The yak hide glue compound semi-finished product is placed in a boiling water bath at a temperature of 90-100°C until it becomes a viscous paste;

(4) Transfer the viscous paste to a smooth flat and shallow concave container, let it stand at room temperature, and after it condenses into a block, take it out and cut it into uniform blocks as required, and dry the block in the shade. The light yellow to dark brown yak hide glue compound Tibetan medicine was obtained.

The yak skin collagen powder in the compound Tibetan medicine of the present invention is a biological product extracted and refined from the fresh skin of the unique animal yak on the Qinghai-Tibet Plateau through modern biotechnology, and is rich in nutrients such as protein and various amino acids needed by the human body. Rhodiola is the dry root and rhizome of Rhodiola crenulata (Hook.f.et Thoms.) H.Ohba, a plant of the Crassulaceae family. Its main active ingredient is salidroside, which is sweet, bitter, and mild. , belonging to the lungs and heart meridians, has the effects of nourishing qi and activating blood circulation, dredging the meridian and relieving asthma; it has obvious effects of anti-fatigue, anti-hypoxia, strengthening the heart and adjusting the metabolism of the human body. Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao is the dry root of Astragalus membranaceus (Fisch.) Bge., a leguminous plant, mainly containing saponins, polysaccharides, various amino acids, Active ingredients such as vitamins and trace elements are sweet in taste and slightly warm, and return to the lung and spleen meridian. And anti-fatigue, anti-aging effects. Ginseng fruit, also known as Juema, is the air-dried root tuber of Potentilla anserina Linn. var. anserina, a plant of the genus Rosaceae. It contains reducing sugar, sucrose, pentosan, starch, protein, tannin, pollin, fat , choline, betaine, histidine, succinic acid, flavonoids and other ingredients, taste sweet, bitter, cold, return to the spleen and stomach meridian, have the effects of nourishing qi and blood, strengthening the spleen and stomach, promoting body fluid and quenching thirst, and promoting dampness. Lycium barbarum is the dry and mature fruit of Lycium barbarum L. of Solanaceae Ningxia Lycium barbarum. Its main active ingredient is Lycium barbarum polysaccharide, which tastes sweet and flat, and returns to the liver and kidney channels. Oxidation, anti-aging, immune regulation, hypoglycemic, hypolipidemic and other effects.

Compared with the prior art, the present invention has the following advantages:

1. The yak skin collagen powder in the present invention is a kind of collagen powder obtained after depilation, dealkalization, water extraction and drying with the skin of Wild Yak , a unique cattle breed in the Qinghai-Tibet Plateau, as raw material. The development of yak skin collagen enables the yak, a unique resource on the Qinghai-Tibet Plateau, to gain a deeper level of value, and at the same time can provide the society with more abundant blood and anti-fatigue products.

2. Pharmacodynamics experiment - anti-fatigue experiment:

Healthy Kunming mice (half male and half female, 4 weeks old, weighing 18-22 grams, provided by the SPF Experimental Animal Center of Gansu College of Traditional Chinese Medicine [certification number is SCXK (Gan)]) were used as experimental animals, and the obtained Low, medium and high doses of yak skin glue compound products, 1.5g/kg/d, 3g/kg/d, 6 g/kg/d, were given to mice in each experimental group by intragastric administration for 7 days, and a blank control group was set up . The result is:

(1) There was no significant difference in the weight of mice between the groups at the beginning of the experiment and at the end of the experiment (P>0.05), and the test substance had no obvious adverse effect on the weight of the animals (see Table 1).

Table 1 The impact of test samples on the body weight of mice

*Compared with the blank group, there is a significant difference (P<0.05)

⑵The weight-bearing swimming test showed that the weight-bearing swimming time of the mice in the high-dose sample group was longer than that of the control group, which was significantly different from the control group (P<0.05), and the weight-bearing swimming test was positive (see Table 2).

Table 2 The influence of test samples on the weight-bearing swimming time of mice

*Compared with the blank group, there is a significant difference (P<0.05)

⑶ Determination of muscle glycogen: the content of muscle glycogen in each dose group in the sample was higher than that of the control group, and there was a significant difference in each dose group (P<0.05) (see Table 3).

(4) Determination of creatine creatine enzyme: in the sample, the creatine creatine enzyme of mice in each dose group was lower than that of the control group, and there was a significant difference between the low and high dose groups (P<0.05) (see Table 3).

(5) Determination of lactate dehydrogenase: In the sample, the lactate dehydrogenase of mice in each dose group was higher than that in the control group, and there was a significant difference between the middle and high dose groups (P<0.05) (see Table 3).

Table 3 Effects of test samples on mouse muscle glycogen, creatine kinase, and lactate dehydrogenase

*Compared with the blank group, there is a significant difference (P<0.05)

In summary, the product of the present invention can significantly improve the weight-bearing swimming time of mice, increase the content of muscle glycogen, reduce creatine creatinase, and improve the enzyme activity of lactate dehydrogenase, thus showing that the product of the present invention has anti-fatigue effect.

3. In the present invention, yak skin collagen powder is reasonably compatible with rhodiola rosea, astragalus, ginseng fruit and wolfberry, and the obtained product has anti-fatigue effect.

4. The product obtained in the present invention is prepared by water extraction, which not only has a simple process and is easy to realize, but also has no additives and is safe to eat.

Detailed ways

Example 1 A yak skin glue compound Tibetan medicine with anti-fatigue effect, the compound Tibetan medicine is made of the following raw materials in parts by weight: 20 parts of yak skin collagen powder, 20 parts of rhodiola rosea, 5 parts of astragalus, and 13 parts of ginseng fruit portion, 10 portions of wolfberry.

Wherein: yak skin collagen protein powder is to make by following method:

① Clean the fresh or pickled yak skin of Wild Yak , a unique cattle breed on the Qinghai-Tibet Plateau, and add 3 times its weight to the yak skin with a sodium hydroxide solution with a concentration of 0.1 mol L ^-1 for 24 hours , to obtain yak hide after depilation.

② Add 3 times the weight of water to the depilated yak skin to wash and soak in turn. The number of soaks is 10 times, soaking for 2 hours each time to remove the residual alkali; when the pH of the soaking solution is 5-9 , that is, dealkalized yak hide is obtained.

③ Cut the dealkalized yak hide into small pieces with a length of 10cm and a width of 5cm, put them into water equivalent to 10 times the weight in grams, boil and extract at a temperature of 90-100°C, and after 3 hours, filter Obtain supernatant A and filter residue A.

④ Add water in milliliters equivalent to 5 times its weight in grams to the filter residue A, and carry out boiling extraction at a temperature of 90-100°C. The number of extractions is 10 times, and each extraction time is 2 hours; after filtering, the combined above Supernatant B and filter residue B; filter residue B discarded.

⑤ The combined supernatant B is spray-dried at an air inlet of 80°C and an air outlet of 70°C, then pulverized and passed through a 100-mesh sieve to obtain yak skin collagen powder.

The preparation technology of this compound Tibetan medicine comprises the following steps:

⑴Preparation of API concentrate:

① Weigh the following raw materials by weight: 20 parts of rhodiola, 5 parts of astragalus, 13 parts of ginseng fruit, and 10 parts of wolfberry.

②After mixing the raw materials in step ①, add water 5 times the total weight of the raw materials, boil and extract at 90-100°C for 1 hour, and obtain supernatant C and filter residue C by filtration.

③ Add 5 times the weight of water to the filter residue C, boil and extract at a temperature of 90~100°C, extract 10 times, and extract for 1 hour each time; after filtering, obtain the combined supernatant D and filter residue D; filter residue D discarded.

④ Heating and concentrating the combined supernatant D at a temperature of 90-100°C to 1/20 of its total volume to obtain the concentrated liquid of the raw material drug.

(2) Add 20 parts by weight of yak skin collagen powder to the concentrated raw material medicine obtained in step (1), heat and dissolve at a temperature of 50-70° C., and stir evenly to obtain a compound semi-finished product of yak skin glue.

(3) The compound semi-finished product of yak hide glue is placed in a boiling water bath at a temperature of 90~100°C until it becomes a viscous paste.

⑷Transfer the viscous paste to a smooth flat and shallow concave container, let it stand at room temperature, take it out and cut it into uniform lumps as needed, and dry the lumps in the shade to get light Yellow to dark brown yak hide glue compound Tibetan medicine.

Example 2 A yak skin glue compound Tibetan medicine with anti-fatigue effect, the compound Tibetan medicine is made of the following raw materials in parts by weight: 24 parts of yak skin collagen powder, 25 parts of rhodiola rosea, 5 parts of astragalus, and 15 parts of ginseng fruit portion, 15 portions of wolfberry.

Wherein: yak skin collagen protein powder is to make by following method:

① After cleaning the fresh or pickled yak skin of Wild Yak , a unique breed of cattle on the Qinghai-Tibet Plateau, add 4 times its weight to the yak skin with a sodium hydroxide solution with a concentration of 0.2 mol L ^-1 for 20 hours , to obtain yak hide after depilation.

② Add 4 times the weight of water to the depilated yak skin to wash and soak in turn. The number of soaks is 8 times, and each soak is 3 hours to remove the residual alkali; when the pH of the soaking solution is 5-9 , that is, dealkalized yak hide is obtained.

③ Cut the dealkalized yak hide into small pieces with a length of 10cm and a width of 5cm, put them into water equivalent to 10 times its weight in grams, and extract by boiling at a temperature of 90-100°C. After 3 hours, filter Obtain supernatant A and filter residue A.

④ Add water in milliliters equivalent to 10 times its weight in grams to the filter residue A, and carry out boiling extraction at a temperature of 90-100°C. The number of extractions is 6 times, and each extraction time is 3 hours; after filtering, the combined above Supernatant B and filter residue B; filter residue B discarded.

⑤ The combined supernatant B is spray-dried at an air inlet of 80°C and an air outlet of 70°C, then pulverized and passed through a 100-mesh sieve to obtain yak skin collagen powder.

The preparation technology of this compound Tibetan medicine comprises the following steps:

⑴Preparation of API concentrate:

① Take the following raw materials by weight respectively: 25 parts of rhodiola, 5 parts of astragalus, 15 parts of ginseng fruit, and 15 parts of wolfberry.

②After mixing the raw materials in step ①, add water 6 times the total weight of the raw materials, boil and extract at 90-100°C for 2 hours, and obtain supernatant C and filter residue C by filtration.

③ Add 5 times the weight of water to the filter residue C, boil and extract at a temperature of 90~100°C, the number of extractions is 6 times, and the extraction time is 2 hours each time; after filtering, the combined supernatant D and filter residue D are obtained; the filter residue D discarded.

④ Heating and concentrating the combined supernatant D at a temperature of 90-100°C to 1/30 of its total volume to obtain the concentrated liquid of the raw material drug.

(2) Add 24 parts by weight of yak skin collagen powder to the concentrated raw material medicine obtained in step (1), heat and dissolve at a temperature of 50-70° C., and stir evenly to obtain a compound semi-finished product of yak skin glue.

(3) The compound semi-finished product of yak hide glue is placed in a boiling water bath at a temperature of 90~100°C until it becomes a viscous paste.

⑷Transfer the viscous paste to a smooth flat and shallow concave container, let it stand at room temperature, take it out and cut it into uniform lumps as needed, and dry the lumps in the shade to get light Yellow to dark brown yak hide glue compound Tibetan medicine.

Example 3 A yak skin glue compound Tibetan medicine with anti-fatigue effect, the compound Tibetan medicine is made of the following raw materials in parts by weight: 30 parts of yak skin collagen powder, 50 parts of rhodiola rosea, 10 parts of astragalus, 30 parts of ginseng fruit portion, 20 portions of wolfberry.

Wherein: yak skin collagen protein powder is to make by following method:

① After cleaning the fresh or pickled yak skin of Wild Yak , a unique cattle breed on the Qinghai-Tibet Plateau, add 3 times its weight to the yak skin with a sodium hydroxide solution with a concentration of 0.4 mol L ^-1 for 15 hours , to obtain yak hide after depilation.

② Add 5 times the weight of water to the depilated yak skin to wash and soak in turn. The number of soaks is 6 times, soaking for 3 hours each time to remove the residual alkali; when the pH of the soaking solution is 5-9 , that is, dealkalized yak hide is obtained.

③ Cut the dealkalized yak hide into small pieces with a length of 10cm and a width of 5cm, put them into water equivalent to 12 times its weight in grams, and extract by boiling at a temperature of 90-100°C. After 4 hours, filter Obtain supernatant A and filter residue A.

④ Add water in milliliters equivalent to 10 times its weight in grams to the filter residue A, and carry out boiling extraction at a temperature of 90-100°C. The number of extractions is 5 times, and the extraction time is 4 hours each time; after filtering, the combined above Supernatant B and filter residue B; filter residue B discarded.

⑤ The combined supernatant B was spray-dried at the air inlet at 80°C and the air outlet at 70°C, then pulverized and passed through a 60-mesh sieve to obtain yak skin collagen powder.

The preparation technology of this compound Tibetan medicine comprises the following steps:

⑴Preparation of API concentrate:

① Take the following raw materials by weight respectively: 50 parts of rhodiola, 10 parts of astragalus, 30 parts of ginseng fruit, and 20 parts of wolfberry.

②After mixing the raw materials in step ①, add water 7 times the total weight of the raw materials, boil and extract at 90-100°C for 3 hours, and obtain supernatant C and filter residue C by filtration.

③ Add 7 times the weight of water to the filter residue C, boil and extract at a temperature of 90-100°C, extract 5 times, and extract for 3 hours each time; after filtering, obtain the combined supernatant D and filter residue D; filter residue D discarded.

④ Heating and concentrating the combined supernatant D at a temperature of 90-100°C to 1/35 of its total volume to obtain the concentrated liquid of the bulk drug.

(2) Add 30 parts by weight of yak skin collagen powder to the crude drug concentrate obtained in step (1), heat and dissolve at a temperature of 50-70° C., and stir evenly to obtain a compound semi-finished product of yak skin glue.

(3) The compound semi-finished product of yak hide glue is placed in a boiling water bath at a temperature of 90~100°C until it becomes a viscous paste.

⑷Transfer the viscous paste to a smooth flat and shallow concave container, let it stand at room temperature, take it out and cut it into uniform lumps as needed, and dry the lumps in the shade to get light Yellow to dark brown yak hide glue compound Tibetan medicine.

Example 4 A yak skin glue compound Tibetan medicine with anti-fatigue effect, the compound Tibetan medicine is made of the following raw materials in parts by weight: 35 parts of yak skin collagen powder, 40 parts of rhodiola rosea, 8 parts of astragalus, 35 parts of ginseng fruit portion, 15 portions of wolfberry.

Wherein: yak skin collagen protein powder is to make by following method:

① After cleaning the fresh or pickled yak skin of Wild Yak , a unique breed of cattle on the Qinghai-Tibet Plateau, add potassium hydroxide solution 5 times its weight and a concentration of 1 mol L ^-1 to the yak skin for 10 hours, Get depilated yak hide.

② Add 8 times the weight of water to the depilated yak skin to wash and soak in turn. The number of soaks is 4 times, and each soak is 4 hours to remove the residual alkali; when the pH of the soaking solution is 5-9 , that is, dealkalized yak hide is obtained.

③ Cut the dealkalized yak hide into small pieces with a length of 10 cm and a width of 5 cm, put them into water equivalent to 15 times its weight in grams, and extract by boiling at a temperature of 90-100 ° C. After 5 hours, filter Obtain supernatant A and filter residue A.

④ Add water in milliliters equivalent to 10 times its weight in grams to the filter residue A, and carry out boiling extraction at a temperature of 90-100°C. The number of extractions is 4 times, and the extraction time is 5 hours each time; after filtering, the combined above Supernatant B and filter residue B; filter residue B discarded.

⑤ The combined supernatant B was spray-dried at an air inlet of 80°C and an air outlet of 70°C, then pulverized and passed through a 90-mesh sieve to obtain yak skin collagen powder.

The preparation technology of this compound Tibetan medicine comprises the following steps:

⑴Preparation of API concentrate:

① Take the following raw materials by weight respectively: 40 parts of rhodiola, 8 parts of astragalus, 35 parts of ginseng fruit, and 15 parts of wolfberry.

②After mixing the raw materials in step ①, add water 10 times the total weight of the raw materials, boil and extract at 90-100°C for 2 hours, and obtain supernatant C and filter residue C by filtration.

③ Add water in milliliters 10 times its weight in grams to the filter residue C, and carry out boiling extraction at a temperature of 90-100°C. The number of extractions is 6 times, and each extraction time is 2 hours; after filtering, the combined supernatant D and Filter residue D; filter residue D discarded.

④ Heating and concentrating the combined supernatant D at a temperature of 90-100°C to 1/40 of its total volume to obtain the concentrated liquid of the raw material drug.

(2) Add 35 parts by weight of yak skin collagen powder to the crude drug concentrate obtained in step (1), heat and dissolve at a temperature of 50-70° C., and stir evenly to obtain a compound semi-finished product of yak skin glue.

(3) The compound semi-finished product of yak hide glue is placed in a boiling water bath at a temperature of 90~100°C until it becomes a viscous paste.

⑷Transfer the viscous paste to a smooth flat and shallow concave container, let it stand at room temperature, take it out and cut it into uniform lumps as needed, and dry the lumps in the shade to get light Yellow to dark brown yak hide glue compound Tibetan medicine.

Example 5 A yak skin glue compound Tibetan medicine with anti-fatigue effect, the compound Tibetan medicine is made of the following raw materials in parts by weight: 40 parts of yak skin collagen powder, 60 parts of rhodiola rosea, 14 parts of astragalus, and 40 parts of ginseng fruit portion, 30 portions of wolfberry.

Wherein: yak skin collagen protein powder is to make by following method:

① After cleaning the yak skin of the fresh or pickled yak Wild Yak , a unique cattle breed in the Qinghai-Tibet Plateau, add potassium hydroxide solution 8 times its weight and 2 mol L ^-1 to the yak skin for 10 hours, Get depilated yak hide.

②Add 10 times the weight of water to the depilated yak skin to wash and soak in turn. The number of soaks is 4 times, soaking for 6 hours each time to remove the residual alkali; when the pH of the soaking solution is 5~9 , that is, dealkalized yak hide is obtained.

③ Cut the dealkalized yak hide into small pieces with a length of 10 cm and a width of 5 cm, put them into water equivalent to 10 times its weight in grams, and extract by boiling at a temperature of 90-100 ° C. After 6 hours, filter Obtain supernatant A and filter residue A.

④ Add water in milliliters equivalent to 10 times its weight in grams to the filter residue A, and carry out boiling extraction at a temperature of 90-100°C. The number of extractions is 6 times, and the extraction time is 6 hours each time; after filtering, the combined above Supernatant B and filter residue B; filter residue B discarded.

⑤ The combined supernatant B was spray-dried at the air inlet at 80°C and the air outlet at 70°C, then pulverized and passed through an 80-mesh sieve to obtain yak skin collagen powder.

The preparation technology of this compound Tibetan medicine comprises the following steps:

⑴Preparation of API concentrate:

① Take the following raw materials by weight respectively: 60 parts of rhodiola, 14 parts of astragalus, 40 parts of ginseng fruit, and 30 parts of wolfberry.

②After mixing the raw materials in step ①, add a few milliliters of water 20 times the total weight of the raw materials in grams, boil and extract at 90-100°C for 6 hours, and obtain supernatant C and filter residue C by filtration.

③Add 10 times the weight of water to the filter residue C, boil and extract at a temperature of 90~100°C, the extraction times are 3 times, and the extraction time is 6 hours each time; after filtering, the combined supernatant D and filter residue D are obtained; the filter residue D discarded.

④ Heating and concentrating the combined supernatant D at a temperature of 90-100°C to 1/50 of its total volume to obtain the concentrated liquid of the bulk drug.

(2) Add 40 parts by weight of yak skin collagen powder to the concentrated raw material medicine obtained in step (1), heat and dissolve at a temperature of 50-70° C., and stir evenly to obtain a compound semi-finished product of yak skin glue.

(3) The compound semi-finished product of yak hide glue is placed in a boiling water bath at a temperature of 90~100°C until it becomes a viscous paste.

⑷Transfer the viscous paste to a smooth flat and shallow concave container, let it stand at room temperature, take it out and cut it into uniform lumps as needed, and dry the lumps in the shade to get light Yellow to dark brown yak hide glue compound Tibetan medicine.

Example 6 A yak skin glue compound Tibetan medicine with anti-fatigue effect, the compound Tibetan medicine is made of the following raw materials in parts by weight: 50 parts of yak skin collagen powder, 70 parts of rhodiola rosea, 15 parts of astragalus, and 50 parts of ginseng fruit portion, 40 portions of wolfberry.

Wherein: yak skin collagen protein powder is to make by following method:

① After cleaning the fresh or pickled yak skin of Wild Yak , a unique cattle breed in the Qinghai-Tibet Plateau, add calcium hydroxide solution 10 times its weight and 1.5 mol L ^-1 to the yak skin for 16 hours , to obtain yak hide after depilation.

② Add 10 times the weight of water to the depilated yak skin to wash and soak in turn. The number of soaks is 4 times, soaking for 12 hours each time to remove the residual alkali; when the pH of the soaking solution is 5-9 , that is, dealkalized yak hide is obtained.

③ Cut the dealkalized yak hide into small pieces with a length of 10cm and a width of 5cm, put it into water equivalent to 5 times its weight in grams, and boil and extract it at a temperature of 90~100°C. After 6 hours, filter Obtain supernatant A and filter residue A.

④ Add water in milliliters equivalent to 20 times its weight in grams to the filter residue A, and carry out boiling extraction at a temperature of 90-100°C. The number of extractions is 2 times, and the extraction time is 6 hours each time; after filtering, the combined above Supernatant B and filter residue B; filter residue B discarded.

⑤ The combined supernatant B was spray-dried at the air inlet at 80°C and the air outlet at 70°C, then pulverized and passed through an 80-mesh sieve to obtain yak skin collagen powder.

The preparation technology of this compound Tibetan medicine comprises the following steps:

⑴Preparation of API concentrate:

① Take the following raw materials by weight respectively: 70 parts of rhodiola, 15 parts of astragalus, 50 parts of ginseng fruit, and 40 parts of wolfberry.

②After mixing the raw materials in step ①, add water 20 times the total weight of the raw materials, boil and extract at 90-100°C for 6 hours, and obtain supernatant C and filter residue C by filtration.

③ Add 20 times the weight of water to filter residue C, boil and extract at a temperature of 90-100°C, extract twice, and extract for 6 hours each time; after filtering, obtain combined supernatant D and filter residue D; filter residue D discarded.

④ Heating and concentrating the combined supernatant D at a temperature of 90-100°C to 1/60 of its total volume to obtain the concentrated liquid of the bulk drug.

(2) Add 50 parts by weight of yak skin collagen powder to the crude drug concentrate obtained in step (1), heat and dissolve at a temperature of 50-70° C., and stir evenly to obtain a compound semi-finished product of yak skin glue.

(3) The compound semi-finished product of yak hide glue is placed in a boiling water bath at a temperature of 90~100°C until it becomes a viscous paste.

⑷Transfer the viscous paste to a smooth flat and shallow concave container, let it stand at room temperature, take it out and cut it into uniform lumps as needed, and dry the lumps in the shade to get light Yellow to dark brown yak hide glue compound Tibetan medicine.

Example 7 A yak skin glue compound Tibetan medicine with anti-fatigue effect, the compound Tibetan medicine is made of the following raw materials in parts by weight: 20 parts of yak skin collagen powder, 20 parts of rhodiola rosea, 5 parts of astragalus, and 10 parts of ginseng fruit portion, 5 portions of wolfberry.

Wherein: yak skin collagen protein powder is to make by following method:

① After cleaning the fresh or pickled yak skin of Wild Yak , a unique cattle breed on the Qinghai-Tibet Plateau, add a barium hydroxide solution 10 times its weight and a concentration of 1.5 mol L ^-1 to the yak skin for 16 hours , to obtain yak hide after depilation.

② Add 10 times the weight of water to the depilated yak skin to wash and soak in turn. The number of soaks is 4 times, soaking for 12 hours each time to remove the residual alkali; when the pH of the soaking solution is 5-9 , that is, dealkalized yak hide is obtained.

③ Cut the dealkalized yak hide into small pieces with a length of 10 cm and a width of 5 cm, put them into water equivalent to 20 times its weight in grams, and extract by boiling at a temperature of 90-100 ° C. After 2 hours, filter Obtain supernatant A and filter residue A.

④ Add water in milliliters equivalent to 20 times its weight in grams to the filter residue A, and carry out boiling extraction at a temperature of 90-100°C. The number of extractions is 2 times, and the extraction time is 1 hour each time; after filtering, the combined above Supernatant B and filter residue B; filter residue B discarded.

⑤ The combined supernatant B was spray-dried at the air inlet at 80°C and the air outlet at 70°C, then pulverized and passed through an 80-mesh sieve to obtain yak skin collagen powder.

The preparation technology of this compound Tibetan medicine comprises the following steps:

⑴Preparation of API concentrate:

① Take the following raw materials by weight respectively: 20 parts of rhodiola, 5 parts of astragalus, 10 parts of ginseng fruit, and 5 parts of wolfberry.

②After mixing the raw materials in step ①, add water 20 times the total weight of the raw materials, boil and extract at 90-100°C for 6 hours, and obtain supernatant C and filter residue C by filtration.

③ Add 20 times the weight of water to filter residue C, boil and extract at 90-100°C, extract twice, and extract for 6 hours each time; after filtering, obtain combined supernatant D and filter residue D; filter residue D discarded.

④ Heating and concentrating the combined supernatant D at a temperature of 90-100°C to 1/60 of its total volume to obtain the concentrated liquid of the bulk drug.

(2) Add 20 parts by weight of yak skin collagen powder to the concentrated raw material medicine obtained in step (1), heat and dissolve at a temperature of 50-70° C., and stir evenly to obtain a compound semi-finished product of yak skin glue.

(3) The compound semi-finished product of yak hide glue is placed in a boiling water bath at a temperature of 90~100°C until it becomes a viscous paste.

⑷Transfer the viscous paste to a smooth flat and shallow concave container, let it stand at room temperature, take it out and cut it into uniform lumps as needed, and dry the lumps in the shade to get light Yellow to dark brown yak hide glue compound Tibetan medicine.

Example 8 A yak skin glue compound Tibetan medicine with anti-fatigue effect, the compound Tibetan medicine is made of the following raw materials in parts by weight: 20 parts of yak skin collagen powder, 20 parts of rhodiola rosea, 5 parts of astragalus, and 10 parts of ginseng fruit portion, 5 portions of wolfberry.

Wherein: yak skin collagen protein powder is to make by following method:

①～④ with embodiment 1～7 .

⑤ Heat and concentrate the combined supernatant B at a temperature of 90~100°C to 1/30 of its total volume to obtain a concentrated solution; put the concentrated solution in a boiling water bath at a temperature of 90~100°C until it becomes a viscous paste, The viscous paste is dried at a constant temperature at 50° C. to constant weight, crushed, and passed through a 60-mesh sieve to obtain yak skin collagen powder.

The preparation process of the compound Tibetan medicine is the same as in Examples 1-7 .

Example 9 A yak skin glue compound Tibetan medicine with anti-fatigue effect, the compound Tibetan medicine is made of the following raw materials in parts by weight: 45 parts of yak skin collagen powder, 35 parts of rhodiola rosea, 10 parts of astragalus, and 25 parts of ginseng fruit portion, 35 portions of wolfberry.

Wherein: yak skin collagen protein powder is to make by following method:

①～④ with embodiment 1～7 .

⑤ Heat and concentrate the combined supernatant B at a temperature of 90~100°C to 1/10 of its total volume to obtain a concentrated solution; put the concentrated solution in a boiling water bath at a temperature of 90~100°C until it becomes a viscous paste, The viscous paste is dried at a constant temperature at 90° C. to a constant weight, crushed, and passed through a 100-mesh sieve to obtain yak skin collagen powder.

The preparation process of the compound Tibetan medicine is the same as in Examples 1-7 .

Example 10 A yak skin glue compound Tibetan medicine with anti-fatigue effect, the compound Tibetan medicine is made of the following raw materials in parts by weight: 50 parts of yak skin collagen powder, 70 parts of rhodiola rosea, 15 parts of astragalus, and 50 parts of ginseng fruit portion, 40 portions of wolfberry.

Wherein: yak skin collagen protein powder is to make by following method:

①～④ with embodiment 1～7 .

⑤ Heat and concentrate the combined supernatant B at a temperature of 90~100°C to 1/20 of its total volume to obtain a concentrated solution; put the concentrated solution in a boiling water bath at a temperature of 90~100°C until it becomes a viscous paste, The viscous paste is dried at a constant temperature at 70° C. to a constant weight, crushed, and passed through an 80-mesh sieve to obtain yak skin collagen powder.

The preparation process of the compound Tibetan medicine is the same as in Examples 1-7 .

Rhodiola rosea is the dried root and rhizome of Crassulaceae plant Rhodiola crenulata (Hook.f.et Thoms.) H.Ohba in the above- mentioned embodiment 1～10 , and Radix Astragali is the leguminous plant Astragalus membranaceus (Fisch. ) Bge.var.mongholicus (Bge.) Hsiao or the dry root of Astragalus membranaceus (Fisch.) Bge., ginseng fruit is the air-dried tuber root of Potentilla anserina Linn. var. anserina in the family Rosaceae, and wolfberry It is the dry and ripe fruit of Lycium barbarum L. of Solanaceae Ningxia wolfberry.

The foregoing embodiments 1-10 are further detailed descriptions of the present invention, but do not imply any limitation to the present invention. Without departing from the above idea of the present invention, various alternatives or changes made according to ordinary technical knowledge and conventional means in the field are included in the present invention.

Claims (7)

1. yak hide glue compound recipe Tibetan medicine with anti-fatigue effect, it is characterized in that: this compound recipe Tibetan medicine is made by the raw material of following weight portion: 20 ~ 50 parts in yak collagen powder, 20 ~ 70 parts of Radix Rhodiolaes, 5 ~ 15 parts of the Radixs Astragali, 10 ~ 50 parts of Herba Herminii, 5 ~ 40 parts of Fructus Lyciis;

Described yak collagen powder is to make as follows:

1. after the yak skin of fresh or the Qinghai-Tibet peculiar cattle kind yak of pickling being cleaned up, in described yak skin, add 3 ~ 10 times of its weight, concentration is 0.1 ~ 2 molL ^-1Aqueous slkali handled the yak skin after obtaining losing hair or feathers 10 ~ 24 hours;

2. the water that adds 3 ~ 10 times of its weight in the yak skin after described depilation cleans successively, soaks, and soaking number of times is 4 ~ 10 times, soaks 2 ~ 12 hours to slough residual alkali at every turn; When the pH value of soak is 5 ~ 9, namely obtain the yak skin of dealkalize;

3. add in the water that is equivalent to 5 ~ 20 times of amount milliliters of its weight grams numbers after the yak skin of described dealkalize being cut into long 10cm, wide 5cm fritter, under 90 ~ 100 ℃ of temperature, boil extraction, after 2 ~ 6 hours, obtain supernatant A and filtering residue A after filtration;

4. in described filtering residue A, add in the water that is equivalent to 5 ~ 20 times of amount milliliters of its weight grams number, under 90 ~ 100 ℃ of temperature, boil extraction, extraction time 2 ~ 10 times, each 1 ~ 6 hour extraction time; After filtration, obtain merging supernatant B and filtering residue B; Described filtering residue B discards;

5. the spray-dried back of described merging supernatant B is pulverized, crossed 60 ~ 100 mesh sieves, namely get yak collagen powder; Or described merging supernatant B heated under 90 ~ 100 ℃ of temperature be concentrated into 1/30 ~ 1/10 of its cumulative volume, obtain concentrated solution; Described concentrated solution carries out boiling water bath under 90 ~ 100 ℃ of temperature, until being viscous pastes, this viscous pastes is pulverized to the constant weight, crossed 60 ~ 100 mesh sieves at constant temperature drying under 50 ~ 90 ℃ of temperature, namely gets yak collagen powder.

2. a kind of yak hide glue compound recipe Tibetan medicine with anti-fatigue effect as claimed in claim 1 is characterized in that: the aqueous slkali of described step in 1. refers to any one in sodium hydroxide solution, potassium hydroxide solution, aqua calcis, the barium hydroxide solution.

3. a kind of yak hide glue compound recipe Tibetan medicine with anti-fatigue effect as claimed in claim 1, it is characterized in that: described Radix Rhodiolae is dry root and the rhizome of the plant bright red Herba hylotelephii erythrosticti of Crassulaceae.

4. a kind of yak hide glue compound recipe Tibetan medicine with anti-fatigue effect as claimed in claim 1, it is characterized in that: the described Radix Astragali is the dry root of leguminous plant Radix Astagali or Radix Astragali.

5. a kind of yak hide glue compound recipe Tibetan medicine with anti-fatigue effect as claimed in claim 1, it is characterized in that: described Herba Herminii is the air-dry tuber of Rosaceae potentilla plants Radix potentillae anserinae.

6. a kind of yak hide glue compound recipe Tibetan medicine with anti-fatigue effect as claimed in claim 1, it is characterized in that: described Fructus Lycii is the dry mature fruit of Solanaceae lycium barbarum.

7. a kind of preparation technology with yak hide glue compound recipe Tibetan medicine of anti-fatigue effect as claimed in claim 1 may further comprise the steps:

⑴ prepare the crude drug concentrated solution:

1. take by weighing following bulk drugs respectively: 20 ~ 70 parts of Radix Rhodiolaes, 5 ~ 15 parts of the Radixs Astragali, 10 ~ 50 parts of Herba Herminii, 5 ~ 40 parts of Fructus Lyciis;

2. after described step each crude drug in 1. being mixed, 5 ~ 20 times of milliliters that add described crude drug gross weight are counted water, boil under 90 ~ 100 ℃ of temperature and extract 1 ~ 6 hour, obtain supernatant C and filtering residue C after filtration;

3. 5 ~ 20 times of milliliters that add its weight in described filtering residue C are counted water, boil extraction under 90 ~ 100 ℃ of temperature, extraction time 2 ~ 10 times, each 1 ~ 6 hour extraction time; After filtration, obtain merging supernatant D and filtering residue D; Described filtering residue D discards;

4. described merging supernatant D is heated under 90 ~ 100 ℃ of temperature and be concentrated into 1/60 ~ 1/20 of its cumulative volume, namely get the crude drug concentrated solution;

⑵ add the yak collagen powder of 20 ~ 50 weight portions in the crude drug concentrated solution of described step ⑴ gained, heating for dissolving under 50 ~ 70 ℃ of temperature stirs, and obtains yak hide glue compound recipe semi-finished product;

⑶ described yak hide glue compound recipe semi-finished product carry out boiling water bath under 90 ~ 100 ℃ of temperature, until being viscous pastes;

⑷ be transferred to described viscous pastes in the poor container of smooth plates scrobicula, leaves standstill under the room temperature, treat its in bulk that condenses after, take out and be cut into as required uniform block, this block namely gets faint yellow to auburn yak hide glue compound recipe Tibetan medicine through drying in the shade.