CN101518336A - Nutrition food with oxidation resistance function and preparation method thereof - Google Patents

Abstract

The invention relates to a nutrition food with oxidation resistance function and a preparation method thereof, belonging to the field of functional foods. The functional nutrition food is mainly prepared by medicine and food dual-purpose materials according to the following parts by weight: 1-5 of Shizhu panax, 1-5 of chrysanthemum, 3-9 of sessileflower acanthopanax barks, 3-9 of tuckahoe, 3-9 of niger seeds, 1-5 of peach kernels, 3-9 of bee pollen, 1-5 of white fungus, 1-5 of blackfungus, 1-5 of monkey's head mushrooms, 2-8 of mushrooms, 1-5 of grape pips, 0.5-1.5 of lycopene and 0.5-1.5 of royal jelly. Results of experiments on animal and human prove that the nutrition food has obvious oxidation resistance function and can also improve the immunization, thereby being suitable for the people needing oxidation resistance, but not suitable for children.

Description

A kind of nutraceutical and preparation method thereof with anti-oxidation function

Technical field

The present invention relates to a kind of nutraceutical and preparation method thereof, belong to field of functional food with anti-oxidation function.

Background technology

Oxidative damage is a key factor that causes human diseases.Oxidation resistant functional nutrient food can prevent or improve the occurrence risk of these diseases.Existence and the growth of body under aerobic conditions, though under physiological conditions, also can produce active oxygen (reactive oxygen speces, ROS).The latter acts on large biological molecule such as nucleic acid, protein and carbohydrate, makes them be subjected to oxidative damage, thereby has started the different lysis of body.Oxidative damage also is one of reason of body aging.Harman (1955) has proposed old and feeble free radical theory.This theory is thought " summation of radical pair body injury is a major reason of body aging ".Be some indexs of investigating the body oxidative damage below

One, lipid peroxide and lipofuscin (also claiming lipofuscin) are the aging biology indexs of setting up according to old and feeble free radical theory.The free radical that body produces in the aerobic process is attacked polyunsaturated fatty acid, generates lipid peroxide.The latter decomposes the generation MDA.MDA can form Schiff alkali with protein and free amino acid generation cross-linking reaction.The latter is difficult for being digested in lysosome, builds up into lipofuscin with age in cell, because of fluorescing and claiming fluorchrome.Because lipid peroxide and lipofuscin all are proportionate with the age at intracellular content, and in the cell inner stablity existence, be easy to mensuration, thereby their content can be used as the biological indicator of interior free yl number.

Two, superoxide dismutase and glutathione peroxidase, these two kinds of enzymes are the enzyme defense system of free radical in the cell.SOD is the enzyme of quencher free radical, and it makes the money and property cancellation speed of superoxide radical improve 10 ¹⁰Doubly, the toxicity of the hydrogen peroxide of disproportionation generation is than 0 ²Much lower.H ₂O ₂Under GSH-Px effect, generate nontoxic water.The activity of SOD and GSH-Px is the main cause that old body is removed the free radical ability drop with descending age in the body.Thereby a kind of oxidation resistant nutraceutical should improve the activity of old body SOD and GSH-Px, reduces number of free radical, reduces gathering of LPO and lipofuscin.

Three, hydroxyproline collagen is a kind of protein of filamentary structure, it by three α peptide chains in the shape of a spiral shape be entwined.Some tissues almost all are made up of collagen as ligament, tail key.Hydroxyproline only exists in collagen, and content is extremely constant, accounts for collagen 12%～14%.Therefore, can be with 7.46 times of amounts of representing collagen of hydroxyproline content or its content.Many bibliographical informations, covalently cross-linked degree increases between the polypeptide chain of old man and geriatric animals collagen, and its solubility reduces, and old man and geriatric animals stiff skin, few elasticity, permeability reduction, wrinkle are increased.And in the skin, tail key hydroxyproline content with reducing age.The reason that hydroxyproline content reduced with age in the collagen also may be relevant with free radical.Radical reaction can cause in the organism macromolecular crosslinked unusually, and hydroxylase is crosslinked and lose the hydroxylation function under the free radical effect in the cell, makes proline can not become hydroxyproline.In addition, the ability of the fibrocyte of synthetic collagen synthetic collagen under the free radical effect reduces, and sure to win quantity is reduced.Therefore, the content of hydroxyproline can be used as the special index of reflection aging course.

After aging is meant that the body growth growth reaches the maturity period, with age, weak, the deficiency of YIN-essence,impairment of YIN-essence of yang-energy, insufficiency of vital energy and blood, function occurring goes down, negative and positive of qi and blood imbalance, the slow property of histocyte, carrying out property, degenerative function reduce and the general performance of the general that gets muddled, my system, incremental deterioration process.Individual aging brings the aging population of entire society, in these years, progressively perfect along with growth in the living standard and medical security system, the aging population process is further quickened, having become humanly in 21 century faced stern challenge, is social concern the most outstanding in the world today.At present, the ratio of over-65s population in developed regions in total population rises to 13.5% of today by 7.0% of nineteen fifty-seven in the world, expecting the year two thousand fifty will reach 24.7%, population at the lower country of fertility-rate (being mainly Europe and north america, Japan, Australia and New Zealand) over-65s has increased to 10%～15% of total population number, expectation this ratio within from now on 30～35 years will be doubled, the aged of the fastest country of aging population (comprising Japan, Germany, Italy) the ratio of total population will near or surpass 40%.

China at present 〉=elderly population had reached 1.3 hundred million in 60 years old, accounted for entire population's 10%, had entered aged type country ranks.Expect the year two thousand twenty, the aged will reach 2.24 hundred million～300,000,000, and wherein the elderly will reach 2,000 ten thousand～30,000,000 advanced age more than 80 years old; The year two thousand fifty, will reach about 400,000,000 China 〉=60 years old old age, account for more than 25% of total population number.Wherein elderly population reach 8,000 ten thousand～1.14 hundred million advanced age more than 80 years old.

Aging population brings many social concerns, therefore, also impels to propose many counter-measures, and wherein very important problem is exactly to delay senility and the prevention and the treatment of geriatric disease.

Delay senility and in fact comprise three implications:the one, increase the longevity, promptly promote longevity, improve the existence age.A large amount of studies show that:delay senility, it is fully possible promoting longevity.Second implication is to improve the elderly's quality of life.Do until old and do not wane.The 3rd implication is prevention and the treatment to old common frdquently encountered disease.Old common frdquently encountered disease has cardiovascular and cerebrovascular disease, diabetes, senile dementia, senile osteoporosis, atonic constipation, the old year gloomy disease of gold etc., increasing in these diseases and old age has substantial connection age, also is the big theme of drug research of delaying senility to these prevention and treatment of diseases.

At present, the various anti-oxidant medicaments in the Chinese and Western of selling on the market all have the effect of certain resisting oxidation and delaying senility.But these Chinese and Western medicines are except also having simultaneously the side effect that varies in size, and effect is also not fully up to expectations.Folk prescription commonly used, the herbal mixture effect neither be clearly.China Ministry of Public Health fitness guru represents that various hormonal therapies are just arranged a long time ago, sheep placenta element, cow fetus agent and so on; Also having research to think long-term replenishes a large amount of hormone to get the probability of cancer higher; Four healthy big foundation stones are rational diet, moderate exercise, smoking cessation limit wine, psychological balance; So a lot of antioxidant and anti-aging advertisements are commercial speculations, flicker consumer's wallet.Therefore research and develop the functional nutrient food of effect edible safety, that have tangible resisting oxidation and delaying senility, can benefit more than one hundred million consumers who needs resisting oxidation and delaying senility.

Summary of the invention

The present invention is directed to the defective in the above-mentioned field, a kind of more efficiently anti-oxidation function nutraceutical that has is provided, its prescription is raw materials used, all are medicine and food raw materials that can be used for nutraceutical production that national Bureau of Drugs Supervision announces, this prescription meets the theory of integration of drinking and medicinal herbs fully, is a kind of natural green nutraceutical.

Purpose of the present invention just provides a kind of prescription of more efficiently anti-oxidation function nutraceutical.

Another object of the present invention has provided the preparation method of this anti-oxidation function nutraceutical.

1, a kind of nutraceutical with anti-oxidation function and preparation method thereof is mainly made by following medicine and food raw materials in portion by weight: stone column genseng 1-5 part, chrysanthemum 1-5 part, Sessileflower Acanthopanax Bark tea 3-9 part, Poria cocos 3-9 part, Semen sesami nigrum 3-9 part, peach kernel 1-5 part, melissa powder 3-9 part, white fungus 1-5 part, black fungus 1-5 part, Hericium erinaceus 1-5 part, mushroom 2-8 part, grape pip 1-5 part, lycopene 0.02-0.06 part, royal jelly 0.5-1.5 part.

2, described nutraceutical, the weight portion of its preferred medicine and food raw materials is: 1 part of stone column genseng, 1 part of chrysanthemum, 3 parts of Sessileflower Acanthopanax Bark tea, 6 parts in Poria cocos, 3 parts of Semen sesami nigrums, 2 parts in peach kernel, 3 parts in melissa powder, 2 parts in white fungus, 2 parts of black fungus, 3 parts of Hericium erinaceus, 3 parts on mushroom, 1 part of grape pip, 0.02 part of lycopene, 0.5 part of royal jelly.

3, the preparation method of described nutraceutical:

(1) take by weighing stone column genseng, Poria cocos etc. 2 flavor medicine and food raw materials in proportion, extract and use 85% alcohol extract, refluxed 3 hours, extract secondary, merging extract vacuum concentrated dry extract 1;

(2) take by weighing Semen sesami nigrum, grape pip, peach kernel etc. 3 flavor medicine and food raw materials in proportion, use 95% alcohol extract, refluxed 3 hours, the extraction secondary, with the extract vacuum concentrated dry extract 2;

(3) take by weighing Sessileflower Acanthopanax Bark tea, Hericium erinaceus, mushroom etc. 3 flavor medicine and food raw materials in proportion, extract, refluxed 3 hours, extract secondary with distilled water, with the extract vacuum concentrated dry extract 3;

(4) take by weighing chrysanthemum, white fungus, black fungus 3 flavor medicine and food raw materials in proportion, use 70% alcohol extract, refluxed 3 hours, extract three times, with the extract vacuum concentrated dry extract 4;

4, merge dry extract 1,2,3,4 it is ground into 100 order powder, remerge melissa powder, lycopene 2 flavor medicine and food raw materials, be ground into 200 order powder jointly, be active component of the present invention.

5, take by weighing the royal jelly medicine and food raw materials in proportion, merge the described active component powder of the present invention of claim 4, after the blending, press the method for production of soft capsule in proportion, be filled into capsule shells.Every soft capsule contains active component 560mg.

6, described preparation method, the dry extract that obtains after concentrating also need is following dry 3 hours at 50-60 ℃.

7, the stone column genseng described in this prescription is meant that main product is in China. Kuan Dian stone pillar area, Liaoning Province, the sylvan life ginseng of ginseng order more than life in 15 years.

8, used Sessileflower Acanthopanax Bark tea in this prescription is to cultivate with the patented technology that we have declared, and the stamen leaf of Sessileflower Acanthopanax Bark tea tree is special.

We are by showing the chemical composition of stone column genseng, chrysanthemum, Sessileflower Acanthopanax Bark tea, Poria cocos, Semen sesami nigrum, peach kernel, melissa powder, white fungus, black fungus, Hericium erinaceus, mushroom, grape pip, lycopene, royal jelly etc. and the modern study of pharmacologically active:

The stone column genseng contains multiple physiologically active ingredient, and wherein ginsenoside is a main functional component in the genseng.Kind of ginsenoside (can be divided into 3 groups isolate 30 altogether from genseng, sun-dried ginseng or white ginseng surplus, be oleanolic acid group, protopanoxadiol group and Protopanaxatriol's group), be called ginsenoside (ginsenoside)-Rx (annotate: x=0, a1, a2, a3, b1, b2, b3, c, d, e, f, g1, g2, g3, h1, h2, h3, s1, s2), panicled fameflower root saponin(e (pseudoginsenoside saponin) F11 etc. is still arranged.Saponin(e is the material base of genseng physiologically active.Protopanoxadiol (protopanaxadiol) and Protopanaxatriol (protopanaxadiol) are the former existence forms in the ginsenoside, when separating aglycon, because the effect of diluted acid, the hydroxyl of molecular side chain part and ethylene linkage cyclization form panoxadiol (panaxadiol) and ginseng triol (panaxadiol), and panoxadiol and panaxatriol all are triterpene compounds.Genseng contains a small amount of volatile oil.In recent years report, the principal component in the volatile oil, low boiling partly is β-elemene (β-elemene); High-boiling fration Panaxynol (panaxynol); Also contain panaxydol (panaxydol) in the volatile ingredient, Panaxynol (panaxytriol), genseng alkynes (ginsenyne) B, C, D, E and α-panacene (α-panasinsene), β-panacene (β-panasinsene), y-elemene (y-elemene), α-Gu Yunxi (α-gurjunene), β-gurjunene (β-gurjunene), rare (the α-neodovene) of α-new cloves three rings, rare (the β-neodovene) of β-new cloves three rings, α-selinene (α-selinene), β-selinene (β-selinene), y-selinene (y-selinene), carypohyllene (caryophyllene) etc.Organic acid and ester class have: citric acid (citric acid), isocitric acid (isocitric acid), fumaric acid (fumaricacid), ketoglutaric acid, oleic acid (oleic acid), linoleic acid (linoleic acid), maleic acid (cis-butendi-carboxylicacid), malic acid (malic acid), pyruvic acid (pyruvic acid), butanedioic acid (succinic acid), tartaric acid (tartaric acid), panax acid (panax acid), salicylic acid (salicyclic acid), vanillic acid (vanillicacid) p-Coumaric Acid (p-hydroxycinnamic), triglycerides (triglyceride), palmitic acid (palmitic acid), tripalmitin (palmitin), α, y-two palmitic acid glyceric acid, Trilinoleyl glyceride, the glycosyl diglyceride.Nitrogen-containing compound has: 17 seed amino acids such as pyrrolidones, choline (choline), atriphos (adenosine triphosphate), adenosine (adenosine), polypeptide and arginine, lysine, glycine, threonine, serine, glutamic acid, asparatate.Carbohydrate has: fructose (fructose), glucose (glucose), arabinose (arabinse), rhamnose (rham-nose), grape grain aldehydic acid (glucuronic acid), mannose (mannose), wood sugar (xylose), sucrose (su-crose), maltose (maltose), gossypose (raffinose) and panose (ginsengtrisaccharide), A, B, C, D.Genseng still contains 38.7% water-soluble polysaccharide and 7.8%～10.0% alkali solubility polysaccharide.Vitamins has: vitamin B1, vitamin B2, cobalamin, vitamin C; Nicotinic acid (nicotinic acid), folic acid (folic acid), pantothenic acid, biotin (biotin), and nicotinic acid amide.Sterol and glycoside thereof have cupreol (β-sitosterol), stigmasterol (stigmasterol), daucosterol (daucosterol), campesterol (campesterol), ginsenoside P[sitosteryl-0 (6-0-fatty acyl)-glucopyranoside] and ester sterol.In addition, genseng contains: adenosine invertase, L-Aspartase, beta amylase, invertase; Maltol (maltol); 20 various trace elements such as Kaempferol (kaempferol), Panasenoside (panasenoside) and copper, zinc, iron, manganese.

Chrysanthemum is the capitulum of composite family herbaceos perennial chrysanthemum Chrysanthemum mori folium Ramat..It is sweet, bitter, cool in nature to distinguish the flavor of.Go into lung, Liver Channel.Function: dispelling wind, heat-clearing, make eye bright, detoxifying cures mainly: headache, dizzy, hot eyes, ambition dysphoria with smothery sensation, treat sore, pyogenic infections.Chrysanthemum contains volatile oil, flavonoid glycoside, chrysanthemum glycosides (chrysanthemin), stachydrine (stachydrine), adenine (adenine), choline (choline), robinin (acacetin), Cobastab ₁And vitamin A sample material, amino acid and trace element etc.Contain borneol (borneol), bornyl acetate (bornylacetate), chrysanthenone (chrysanthenone), camphor (camphor) etc. in the volatile oil.In addition, still contain aminozide (aminozide).This product is the dry capitulum of feverfew.Spend September to November and gather when in full bloom in batches, dry in the shade or dry, or smoked, dry hyoscine after steaming.Medicinal material is pressed the habitat processing method difference, is divided into " milli chrysanthemum ", " Chu chrysanthemum ", " tribute chrysanthemum ", " Hang Ju " etc.Chrysanthemum originates in China, and cultivation history is long, and the record of " moon of the last month of autumn, bring up Huang Hua " is arranged in " Book of Rites ".People from Shanxi Fu Xuan also once composed, and claimed chrysanthemum " person's longevity of clothes, the person of food is logical refreshing ".Song dynasty great writer Soviet Union and Eastern Europe slope, even throughout the year all the food chrysanthemum, he eat seedling in the spring, summer food leaf, the autumn anthophagy, the winter is eaten root.The anti-oxidation function of chrysanthemum is the influence of controlled observation chrysanthemum water extract spontaneous lipid peroxidation (LPO) reaction during to the rat heart, brain tissue incubated in vitro with the red sage root; To mouse core, brain homogenate and the heart of hydroxy radical (OH) initiation, the influence of brain line body film LPO reaction.The result shows that chrysanthemum water extract is suitable to the inhibitory action and the red sage root of said process; Mouse continuous irrigation stomach chrysanthemum water extract 2g/ (kgd), 4g/ (kgd), 15g/ (kgd), its heart, brain tissue LPO product mda content all significantly are lower than the physiological saline group.The above results shows that chrysanthemum water extract has the effect that free radical generated and suppressed the LPO reaction of free radical initiation that suppresses.Chrysanthemum is to the influence of the old and feeble Experimental Study on Antioxidation chrysanthemum of D-galactolipin water extract to mice serum SOD, GSH-Px, MAO vigor and MDA content.Model group and control group relatively, SOD, GSH-Px vigor all significantly reduces (P＜0.01), and MAO vigor and MDA content be significantly increased (P＜0.01); Experimental group and model group compare, and the vigor of SOD, GSH-Px is significantly increased (P＜0.01), and MAO vigor and all significantly reductions (P＜0.01) of MDA content.Experimental result shows, chrysanthemum water extract can obviously resist lipid peroxidation due to D-galactolipin, reduces MDA content, MAO vigor in the blood; Improve SOD, GSH-Px vigor in the blood; Strengthen body to measured by esr technique, the process that delays senility is improved body condition, and have certain strengthen the body resistance to consolidate the constitution, anti-ageing anti-old effect.Point out the effect of chrysanthemum aspect anti-ageing thus, may to remove interior free yl relevant with chrysanthemum.

Contain eleutheroside A (eleutheroside eleutheroside B (Syringin syringin), eleutheroside B1 (6,8-escoparone-7-glucoside), eleutheroside L (galactite glycosides), eleutheroside D and E (be two kinds not the purple fourth of isomorphism type get the glucoside of Syringaresinol) etc. in the Sessileflower Acanthopanax Bark tea Sessileflower Acanthopanax Bark.In addition, still contain polysaccharide and 1-sesamin (1-sesamin).The anti-oxidation function of Sessileflower Acanthopanax Bark, Chen Yue are inquired into the effect of wilsonii saponin(e (ASS) to the damage of x-ray bombardment mouse.50 of mouse are divided into negative control group, positive controls and basic, normal, high dosage ASS experimental group at random.Measure mouse spleen index, thymus index, superoxide dismutase (SOD) and glutathione peroxidase (activity of GSH-PX), and mensuration lymphocyte transformation rate and peripheral white blood cell.The result shows, 3 every indexs of ASS experimental mice and control group relatively, spleen index difference does not have conspicuousness (P＜0.05), thymus index, LSI, SOD in serum and GSH-PX activity difference all has conspicuousness (P＜0.05).Conclusion: ASS has certain radioresistance and antioxidation to radiation murine.Sessileflower Acanthopanax Bark has anti-aging effects, and the oral wilsonii of rat is after 2 months, and the cytolipin oxide content reduces, Na ⁺, K ⁺-atpase activity raises.This product ethanol extract can prolong the time-to-live that mouse ligation bilateral common carotid arteries or broken end are panted; Can reduce the level of lipid peroxide in rats'liver, the brain, improve the activity of SOD (superoxide dismutase) in the blood plasma, the lipid peroxide content increase of pouring into again behind the ischemic in the brain is had protective effect.

Poria cocos is the dry sclerotia of Polyporaceae plant Poria cocos Poria cocos (Schw.) Wolf.It is sweet, light to distinguish the flavor of, and property is flat.The thoughts of returning home, spleen, lung channel.Function: eliminating dampness and diuresis, beneficial spleen and stomach, antitoxic heart-soothing and sedative.Cure mainly: difficult urination, oedema turgor, phlegm and retained fluid are coughed contrary, n and V, are had loose bowels, seminal emission, stranguria with turbid discharge, palpitation with fear, forgetful.Poria cocos mainly contains B-pachyman (B-pachyman), account for 93% of dry weight, triterpene compound pachymic acid (pachymic acid), soil are herded acid (tumulosic acid), triterpene carboxylic acid (triterpene carboxylicacid), eburicoic acid (eburioic acid), loose Siberian cocklebur acid (pinicolic acid) etc.Also fatty acids is as sad (caprylicacid), undecanoic acid (undecanoic acid), laurate (lauric acid), dodecoic acid (dodecenoic acid) and palmitic acid (palmitic acid).In addition, still contain ergosterol (ergosterol), natural gum, chitin, protein, fat, sterol, lecithin, D-glucose, adenine (adenine), histidine (histidine), choline (choline), B-pachyman catabolic enzyme, lipase, protease etc.The old and feeble effect of the anti-animal of anti-oxidant and deferring senility research pachymaran of Poria cocos.Adopting normal mouse and old rats is experimental animal, observes pachymaran influence active and cold-resistant to animal and that antifatigue is tested to MDA (MDA) content, superoxide dismutase (SOD) and monoamine oxidase (MAO).The result shows that pachymaran can increase T-SOD and Cu-SOD activity in the serum in various degree, reduces MDA content, but the MAO activity is not then had obvious influence.Pachymaran can also delay the mouse swimming death time.Conclusion: pachymaran has the old and feeble effect of anti-preferably animal.Inquire into Poria cocos to superoxide dismutase (SOD) activity in the senile rat red blood cell, the influence of SOD activity and hydroxyproline content in the skin, and then the mechanism of supposition Poria cocos delaying decrepitude of skin.The Wistar rat is divided into dosage group, Poria cocos high dose group in the young control, aged blank group, positive controls, Poria cocos low dose group, Poria cocos.Give physiological saline (20ml/kg) respectively, physiological saline (20ml/kg), vitamin E (2g/kg), Poria cocos extract 2g/kg, 4g/kg, 8g/kg irritate stomach.The detection corresponding index of drawing materials behind the 30d.The result shows that the hydroxyproline content of each group all is higher than old blank group (P＜0.05). the no significant difference of the active and old blank group of SOD in red blood cell in Poria cocos low dose group, the Poria cocos in dosage group, the Poria cocos high dose group and the skin (P〉0.05).Conclusion: Poria cocos may be to come delaying decrepitude of skin by the content that improves hydroxyproline in the skin, and the activity of SOD is not influenced.

Semen sesami nigrum is a Pedaliaceae plant sesame Sesamum indicum DC. black seed, also name flax, huge victory (" herbal classic "), black fiber crops, black pockmarks (Prescriptions Worth Thousand Gold for Emergencies), spunyarn (" dietetic materia medica "), Semen sesami nigrum (" ternary lengthen the life book ").It is sweet to distinguish the flavor of, and property is flat.Return liver, kidney channel.Function: filling liver kidney, moisturizing viscera.Cure mainly: kidney deficiency and liver, empty wind are dizzy, wandering arthritis, paralysis, dry feces, empty thin, poliosis, married woman's breast are few after being ill.The fatty oil of Semen sesami nigrum seed can reach 60%.The glyceride that contains oleic acid, linoleic acid, palmitic acid, arachidic acid, carnaubic acid, twenty diacid etc. in the oil.Also contain sterol, sesamin (sesamin), sesamol (sesamol), sesamolin (sesamolin), vitamin E etc.Still contain the calcium of folic acid 18.45%, nicotinic acid 0.48%, sucrose 0.64%, lecithin 0.65%, pentosan, protein and volume etc. in addition.The anti-oxidation function of Semen sesami nigrum adopts chemiluminometry, and it is luminous and remove the research of active oxygen effect that the pigment that extracts from Semen sesami nigrum and polysaccharide are resisted whole blood.The result shows, the Semen sesami nigrum polysaccharide is eager to excel to the active oxygen effect that the chemiluminescent resistancing action comparison of whole blood acellular system produces, and the active oxygen that Semen sesami nigrum pigment pair cell or acellular system are produced all has stronger scavenging action, the ability that prompting Semen sesami nigrum polysaccharide is directly removed active oxygen a little less than, and phagocyte is had certain immune resistancing action.And the Semen sesami nigrum element is one of free radical scavenger stronger in the Semen sesami nigrum composition.

Peach kernel is the seed of rosaceous plant peach Prunus Persica (L.) Batsch or mountain peach Prunusdavidiana (Carr.) Franch..Bitter, sweet, property is flat.Go into the heart, liver, large intestine channel.Function: the broken capable laxation that becomes silted up, moisturizes of blood.Cure mainly: through Bi, wei lump in the abdomen, wandering arthritis, malaria, traumatic injury, extravasated blood swell and ache, the dry constipation of blood.Main component contains amarogentin (amygdalin), emulsin (emulsin), allantoinase (allantoinase), lactase (lactase), Cobastab ₁, mainly contain olein and a small amount of glyceryl linoleate in the volatile oil, fat oil, oil.Emulsin comprises amygdalase (amygdalase) and prunase (prunase).Amarogentin in the peach kernel is the same with amarogentin in the almond, produces hydrogen cyanide, benzaldehyde and glucose after enzyme or acid hydrolysis.The medicinal efficacy of peach kernel is mainly reflected in anti-oxidant aspect: observe all ages and classes rat body lipid peroxide (LPO) and this enzyme of red blood cell superoxide dismutase (SOD) content.The result shows that rat is with age growth, and LPO content raises in blood plasma and liver, the brain tissue.SOD is active in the red blood cell descends.But feed and to raise the old rats of walnut kernel after 3 months and compare with control group.LPO content decline (P＜0.05).The SOD activity increases (P＜0.05), and liver, brain tissue homogenate's in vitro culture are starkly lower than control group (P＜0.01) with the LPO in the walnut kernel test tube.The result shows: walnut kernel truly has certain antioxidation.

Melissa pink blossom powder is the reproduction cell that the stamen in the plant flower produces, and is the Source of life of plant.It is not only carrying the hereditary information of life, and comprises and breeding the necessary complete nutrients matter of new life.Contain 20 seed amino acids, 30 kinds of organized enzymes, multivitamin, mineral matter, crude fibre, nucleotides etc.The fat of pollen form found laurate, tetradecanoic acid, palm resin acid, octadecanoid acid, arachidic acid, oleic acid, heptadecanoic acid, linoleic acid, leukotrienes and other aliphatic acid arranged, wherein, noticeable also have irreplaceable unrighted acid---linoleic acid plus linolenic acid, and quantity accounts for half.The phosphatide of pollen has choline phosphoglyceride, inositol monophosphate glyceride, ethylaminoethanol phosphoglyceride (cephalin), phosphatidyl chlorine propylhomoserin etc., and it has a lot of important physical functions.Anti-oxidation function adopts the excusing from death method, it with methyl alcohol, water respectively the melissa powder active component of solvent extraction rape and lotus, extract by measuring rape and Flos Nelumbinis pollen is estimated the oxidation resistance of two kinds of melissa powder to the clearance rate of ultra-oxygen anion free radical, hydroxyl radical free radical and to the resistance rate of lipid peroxidation.The result shows: two kinds of bee pollen extracts all have stronger antioxidation, and Brassica campestris L pollen extract has stronger non-oxidizability than Flos Nelumbinis pollen extract.The bee pollen form cole methanolic extract can reach 83.2%, 99.2% to ultra-oxygen anion free radical, hydroxyl radical anion clearance rate, can reach 92.2% to the resistance rate of lipid peroxidation.

The white fungus white fungus (Tremella fuciformis Berk.) claim tremella again, is under the jurisdiction of Basidiomycotina Hymenomycetes, Heterobasidiomycetae, Tremellales (Tremellales), Tremellaceae, Tremella.White fungus is a kind of famous food and medicament dual-purpose bacterium of China.China's successive dynasties medicine scholar thinks, white fungus has the effect of " kidney tonifying, moistening lung, promote the production of body fluid, cough-relieving ".According to Zhang Renan " herbal poem separate the property of medicine annotate " cloud, " this thing has the profit of the tuber of dwarf lilyturf and does not have that it is cold, the sweet of radix polygonati officinalis arranged and does not have that it is greasy, and really the moistening lung enriching yin will product ".White fungus contains protein 6.7%～10%, carbohydrate 65%～71.2%, fat 0.6%～12.8%, crude fibre 2.4%～2.75%, inorganic salts 4.0%～5.4%, moisture 15.2%～18.7% and a small amount of vitamin(e) B group.Contain 18 seed amino acids in the white fungus protein, i.e. leucine, isoleucine, phenylalanine, valine, tyrosine, proline, arginine, lysine, alanine, threonine, glycine, serine, glutamic acid, aspartic acid, cystine, histidine, dl-methionine and Gamma Amino Butyric Acid.White fungus is former to be jelly fungus on the wild withered tree, and China's white fungus is based on artificial cultivation in recent years.At present about China white fungus annual production 8000～10000t, first of the per unit area yield and the gross output Jun Ju world.Tremella polysaccharides is the major physiological active material that extracts from white fungus, has many-sided health-care effect.

The black fungus black fungus is a kind of of auricularia auriculajudae.Because of it is grown on the rotten wood, likeness in form people's ear, Se Hei or brown black is so the name black fungus has another name called wood fungus, tree chicken.This product is edible both, can be used as medicine again, is the product of dietotherapeutic.Black fungus comes from the fructification of Auriculariaceae fungi auricularia auriculajudae, Uricularia polytricha or wrinkle auricularia auriculajudae, and multiparasitization was wild on dry and decayed limb such as mulberry, oak, elm, poplar, Chinese scholartree originally, now mostly was cultivation.Existing pharmacy monograph, the Han dynasty the earliest of China (Shennong's Herbal has just been put down in writing its medical value.This book is cloud under the Cortex Mori bar: five auricularia auriculajudaes energy " beneficial gas is not hungry, the strong will of making light of one's life by commiting suicide "; Cloud Sang Erhei person again, " lame lump in the abdomen is gathered for main woman's whitish metrorrhagia juice, blood disease, cloudy pain, negative and positive fever and chills, s.m.p." above said five auricularia auriculajudaes comprise the black fungus of the present; and Sang Erhei person is a kind of successive dynasties book on Chinese herbal medicine of black fungus of the present as " dietetic materia medica ", the Compendium of Material Medica of the Ming Dynasty of Northern and Southern Dynasties' beam " Mingyi Bielu " in generation, the Tang Dynasty, contemporary " Chinese this stroke " etc., and it is all had argumentation.Put down in writing according to " China's book on Chinese herbal medicine ": the sweet property of black fungus is flat, returns spleen, lung, liver, large intestine channel.Cure mainly Qi and blood deficiency, chronic cough of deficiency lung, spitting of blood, hemorrhoid hemorrhage, woman uterine bleeding, irregular menstruation, savate damage etc.Modern experimental study proves, black fungus mainly contains protein, fat, lecithin, sphingomyelins, multivitamin, and inorganic constituents such as calcium, phosphorus, iron, has anticoagulant, antithrombotic forms, promote immunologic function, regulate blood fat, antiatherosclerosis, hypoglycemic, delay senility antiulcer, pharmacological action widely such as antimycotic grade.

Hericium erinaceus Hericium erinaceus formal name used at school Hericium erinaceus (Bull.Fr.) Pers. popular name: Hericium erinaceus, hedgehog hydnum, hedgehog hydnum mushroom, perverse hedgehog bacterium, wool bacterium, monkey mushroom bacterium.Hericium erinaceus is under the jurisdiction of Basidiomycetes, Aphyllophorales, hedgehog hydnum Cordycepps, Hericium.Hericium erinaceus property is flat, and it is sweet to distinguish the flavor of.Be good for the stomach, qi-restoratives, anticancer.The traditional Chinese medical science thinks, the flat flavor of Hericium erinaceus property is sweet, favourable the five internal organs, aid digestion, effect such as nourish the body.Hericium erinaceus is the traditional precious edible mushroom of China, with bear's paw, sea cucumber, shark's fin and title " four big famous dishes ", and saying of " mountain delicacy hedgehog hydnum, seafood delights bird's nest " is arranged.Since the seventies in 20th century, modern medicine proves that successively Hericium erinaceus has good medical value, and clinical practice shows that Hericium erinaceus can be treated diseases such as indigestion, gastric ulcer, antral gastritis, stomachache, gasteremphraxis and neurasthenia.Research in recent years shows that also Hericium erinaceus has antitumaous effect.It is reported that Hericium erinaceus has significant inhibitory effect to small white mouse sarcoma S-180, on probation to malignant tumor of digestive tract patients such as 166 routine cancer of the stomach, cardia cancer, cancers of the esophagus, efficiently reach 69.3%, produce effects 15%.Suitable suffer from stomach trouble, comprises chronic gastritis, and the gastric and duodenal ulcer person is edible; Suitable having a delicate constitution, malnutrition, the neurastheniac is edible; The people of suitable pain disease patient, especially cancer of the esophagus, cardia cancer, cancer of the stomach is edible; Also suitable cardiovascular patient is edible.The Hericium erinaceus qi-restoratives is good for the stomach, and all nothings are avoided.Hericium erinaceus is described as the health food of " plant meat " by people.Measure according to Beijing food research institute, in the dried Hericium erinaceus of 100 grams, contain protein 26.3 grams, exceed 1 times than mushroom; In 16 contained seed amino acids, there are 7 kinds to be essential amino acid.Contained fat mass is less.Other contains provitamin A, vitamin B1, vitamin B2 and niacin, polysaccharide body, polypeptide class and microelements of calcium, phosphorus, fast, and nutritional labeling such as food fiber.According to clinical research confirmation, after the cancer patient used the Hericium erinaceus polysaccharide body, body had produced interferon, has strengthened anticancer effect.Normal food Hericium erinaceus, can also raise human immunoglobulin and leucocyte strengthen immune function of human body, so quite helpful to pain disease patient.

Mushroom mushroom (Lentinus edodes) has another name called dried mushroom, belongs to Basidiomycetes, Agaricales, Pleurotaceae, Lentinus, and its output accounts for that the world is edible eats more than 15% of class total output, is only second to two spore mushrooms, is the second largest class edible mushroom of cultivation.Mushroom gives off a strong fragrance, delicious flavour, nutritious, and the characteristics that have high protein, low fat, low in calories, multivitamin, amino acid and deposit have strengthening the spleen and replenishing qi, strengthening vital QI to eliminate pathogenic factors, dispelling wind to loose to become silted up, the effect of YIN and YANG balance regulating, are the good merchantable brands in food, the medicinal bacterium.Modern medicine study finds that often edible Xianggu-mushroom has the cholesterol of reduction, prevents vascular sclerosis, promotes upgrowth and development of children, strengthens effects such as immune function of human body and anti-curing cancers.Lentinan is a major physiological active material wherein, has remarkable enhancing cancer patient's various biological functions such as resistance.Clearly there is immunocompetent lentinan to have only beta glucan one class.

The grape pip grape is nutritious, is rich in nutritional labelings such as mineral matters such as potassium, iron, boron, vitamin B1, vitamin B2, nicotinic acid, vitamin E, tartaric acid, oxalic acid, citric acid.Property is sweet, sour, goes into spleen, lung, kidney channel, has multiple treatment and health effect.The traditional Chinese medical science thinks that its fruit, root and rattan all can be used as medicine.(grape seed extract contains the chemical composition that a large amount of Polyphenols, lipid and mineral substance etc. are of value to human body in EGS) to grape seed extract, and multiple disease is had prevention and therapeutic action.Early on the books in the document: " book on Chinese herbal medicine is new again ": " warm stomach invigorating the spleen controls that the deficiency syndrome of the lung is cold coughs, the long-pending subcutaneous ulcer knurl of broken blood." " occupying the diet spectrum " with breath: " and tonifying Qi, nourshing kidney liquid, beneficial liver-yin, strengthening the bones and muscles is quenched the thirst, and is antiabortive." " hundred careless mirrors ": " control the wet pain of muscles and bones, Li Shui is very prompt, removes edema all over." " Bencao Tujing ": " gas is sent out the sore rash and is not gone out the person when controlling, and grinds beverage." " Luchuan book on Chinese herbal medicine ": " nourish strongly, enrich blood the cardiac stimulant diuresis.Control pain in the back, stomachache, neurolysis, deficiency of blood heartbeat." Shennong's Herbal: " strong will is made us fertile strong anti-famine, bears chill for main muscles and bones arthritis with fixed pain caused by dampness, beneficial gas times power." " the southern regions of the Yunnan Province book on Chinese herbal medicine ": " strongly invigorating QI and blood is stimulated the circulation of the blood and cause the muscles and joints to relax, the clothes of steeping in wine.Control negative and positive and take off disease, control the night sweat chronic diseases marked by deficiency of vital energy again.Juice is controlled cough." contain polyphenols (GSP) in the chemical composition polyphenols grape pip in the grape seed extract, mainly contain catechin and OPC class.Catechin compounds comprises catechin, epicatechin and gallate thereof, is single aggressiveness main in the grape pip, also is OPC oligomer and polymeric component unit.Grape seed extract OPC (GSPE) is that the flavanols by different numbers is polymerized, and what research was the widest at present mainly is by (+)-catechin ((+)-catechin), (-)-epicatechin (this compounds that (-)-epicatechin) forms for monomer polymerization.Two are called oligomer (OPC) to the tetramer, are called high polymer (PPC) more than the pentamer.Its biologically active increases, strengthens with the degree of polymerization.The bioflavonoid OPC that extracts from grape pip compares with most of flavones, and structure is special, good water solubility, and the validity height, bioavilability very easily is absorbed by the body more than 90%.Contain 10%～15% grape-kernel oil approximately in lipid and other chemical composition grape pips, its main component is that multiple unrighted acid such as linoleic acid, leukotrienes and sterol etc. and polyhydroxy class (PHS) are as resveratrol etc.It is higher to contain mineral element potassium, sodium, calcium and iron amount in the grape-kernel oil, and contains multivitamin.The phenolic acid class mainly comprises protocatechuic acid, coumaric acid, gallic acid, caffeic acid and syringic acid etc.Physiologically active antioxidation GSPE can remove excessive free radicals effectively, and oxidative damage is had protective effect.Experiment in vitro adopts chemoluminescence method and cromoci reducing process to measure the ability that GSPE, vitamin C, vitamin E, beta carotene, superoxide dismutase (SOD), catalase and sweet mellow wine are removed ultra-oxygen anion free radical and hydroxy radical.The result shows, GSPE inhibition ability to ultra-oxygen anion free radical and hydroxy radical when 100mg/L concentration is 78% and 81%, vitamin C is 12% and 19%, vitamin E is 50% and 75%, SOD and catalase synergy suppress ultra-oxygen anion free radical and are about 83%, sweet mellow wine suppresses hydroxy radical and is about 87%, illustrates that GSPE has than above-mentioned two kinds of oxidation resistances that vitamin is strong.GSPE can reduce the vitamin E free radical and make vitamin E regeneration, thereby reduces the loss of vitamin E in the cell.GSPE is used in combination with vitamin E and can obviously postpones the conjugated diene that lecithin lipid peroxidation that hydroxy radical induces forms, and shows that GSPE has the obvious synergistic humidification to the antioxidation of vitamin E.To rat feeding GSPE high cholesterol-scarce vitamin E feed, catalase, glutathione peroxidase and SOD activity can obviously return to the level of the normal feed rat of feeding in its sustainer, the heart, liver, intestines, muscle and the kidney, show that GSPE can strengthen the ability of body tissue antioxidase system, thus the enhancement antigen ability.PHS also can make rabbit anteserum SOD, glutathione peroxidase content raise, and improves the activity of body antioxidase, reduces the generation of lipid peroxide.

Lycopene lycopene (lycopene) is kermesinus powder or oily liquids, and oil solution is yellowish orange, belongs to carotenoid, and pure product are needle-like peony crystal (from the mixed liquor person of separating out of carbon disulfide and ethanol).174 ℃ of fusing points, flammable.Heat-resisting good with light resistance.Water insoluble, be soluble in carbon disulfide (1g/50ml), boiling diethyl ether (1g/3L), normal hexane (1g/14L, 0 ℃), be dissolved in chloroform and benzene; Be slightly soluble in ethanol and methyl alcohol.Because of belonging to fat-soluble pigment, can be absorbed and used after being dissolved in grease.Content is many especially in the ripe tomato.Along in the fruit of the pulp, the grape fruit that are present in the big pimiento of lantern, cynarrhodion, watermelon, guava, Convallariae majalis fruit, persimmon, pumpkin and the rhodobacterium etc.Its anti-oxidation function lycopene is to the protective effect of rat oxidative damage due to the ozone.Utilize rat to suck ozone and make damage model, it is mutual to irritate the stomach tomato red simultaneously, 6 week the back detect that superoxide dismutase (SOD) in rat blood serums and the liver is active, glutathione peroxidase (GSH-Px) vigor and MDA (MDA) content.Show that the high and low dose group is compared with the damage model group, SOD and GSH-Px vigor raises, and MDA content descends, significant difference (P＜0.05).Conclusion: lycopene has the certain protection effect to rat oxidative damage due to the ozone.Observe the influence of lycopene to the rat anti oxidase system.Put to death feed lycopene 4 week back of rat, and the superoxide dismutase (SOD) of measuring serum and LH is active, catalase (CAT) is active, glutathione peroxidase (vigor of GSH-Px), TAC (T-AOC), MDA (MDA) and reduced glutathione (GSH) content.The result shows that the rat blood serum of each lycopene administration group and the SOD activity of hepatic tissue, GSH-Px vigor and T-AOC rises, and MDA content reduces, and compares with the blank group, and difference has conspicuousness (P＜0.05) statistically.Conclusion: lycopene can improve the oxidation resistance of rat body, resists lipid peroxidation.The CCI300 free radical model that deferring senility adopts the impulse radiation technology to produce, the Synergistic relation when investigating natural VE and lycopene and this radical reaction.On this basis, be that main component is made into composite antioxidant with these two kinds of antioxidants, utilize fruit bat, old rats animal model for survival.Investigated of the influence of this composite antioxidant respectively to superoxide dismutase (SOD) activity and lipid peroxide (LPO) content in life span of drosophila melanogaster, the rat blood serum.The result shows that this composite antioxidant has collaborative effect of removing free radical; Can obviously prolong average life span and the maximum life span of fruit bat; Function with remarkable enhancement of SOD activity and reduction LPO.Point out this composite antioxidant to have certain anti-oxidant, function in delaying senility.

The nutritive value of royal jelly royal jelly is higher than honey.Contain moisture in the royal jelly up to 2/3, all the other are protein, amino acid, inorganic salts and B family vitamin, also contain bioactivators such as a spot of nucleotides, hormone, enzyme in the royal jelly.Because the royal jelly taste is not good enough, has pungent astringent taste, it is edible that general normal honey with 10 times mixes the back; Also can be made into formulations such as tablet, capsule or oral liquid takes.Main component is that 10-hydroxyl-2-decylenic acid (also claims royal jelly acid, be called for short 10-HAD, contain 2% approximately, C10H1803,186.25), other contains moisture 62.5%～68.5%, protein 11.0%～14.5%, multivitamin, adenosine are arranged, and (UDP-glucose, UDP-gluconic acid, UD-Px), uridine, guanosine, cytidine have enzymes such as acidity and alkaline phosphatase, cholinesterase.Milky white have sour and astringent acid to faint yellow slurry like material, slightly sweet, has heat-resisting, soluble in water.

Nutraceutical with anti-oxidation function disclosed by the invention, it mainly is to be prepared from according to a certain weight ratio by medicine-food two-purpose remedies post genseng, chrysanthemum, Sessileflower Acanthopanax Bark tea, Poria cocos, Semen sesami nigrum, peach kernel, melissa powder, white fungus, black fungus, Hericium erinaceus, mushroom, grape pip, lycopene, royal jelly etc., and the some of them medicine-food two-purpose medicine of this prescription plays function in delaying senility by anti peroxidation of lipid and removing oxygen radical.The effect that the water-soluble composition of stone column genseng alcohol extract active component and Sessileflower Acanthopanax Bark tea, Hericium erinaceus, mushroom has very strong anti peroxidation of lipid and removes oxygen radical.The elderly's basic metabolism level is low, makes body obviously descend accelerated ageing to the function of metabolism.Genseng, chrysanthemum, peach kernel etc. can reduce blood sugar, and the effect that reduces blood fat is arranged again, can regulate the glycometabolism and the lipid-metabolism of body.Medicine-food two-purpose medicine genseng, Semen sesami nigrum, melissa powder, Hericium erinaceus, mushroom, royal jelly with the function of tonifying the kidney and support yang have the inhibition monoamine oxidase (MAO-B) effect of activity, improve old and feeble brain symptom in addition, its mechanism is by improving the binding capacity of some neurotransmitter receptor, and the approach such as activity that change some enzyme realize.The warming kidney and enhancing yang medicine of the medicine-food two-purpose in the prescription can improve the elderly's kidney-yang deficiency symptom, improves to be worth the reaction that reaches somatotropin releasing factor (GRF) nictation, produces the pituitrin effect, can make the low T of the patient that suffers from a deficiency of the kidney simultaneously ₃, T ₄Content raises, and produces the effect to thyroid hormone; Genseng, Semen sesami nigrum, melissa powder, royal jelly studies show that to the effect of sexual gland kidney-nourishing yang-strengthening medicine can be urged gonad development and function in the prescription.Mouse peritoneal injection stone column genseng, melissa powder extract can obviously increase the effect of plasma testosterone, and obviously promote testis tissue hyperplasia and secretion, also can obviously increase the weight in adenohypophysis, ovary and uterus, and ovary HCC/LH receptor-specific adhesion obviously improves.Medicine-food two-purpose medicine grape pip in the prescription, lycopene etc. show the effect test of thymus gland, all can improve mouse thymus weight, improve the Ultrastructural senile variation of thymus gland, increase the content of thymus gland plasmosin, nRNA and DNA, delay the degeneration of thymus function, improve old TD immunologic function.This prescription is tried thing can make the gene expression dose of senile rat codase albumen strengthen, thereby has improved the active of SOD and CAT and to the repair of gene damage; Can also keep the gene normal expression and delay senility by improving the dna methylation level; Can obviously improve the synthetic level of old rats DNA, improve the content of DNA, nucleic acid in the bone marrow nucleated cell, improve the archaeal dna polymerase vigor, can also extract mouse liver dna methylation enzyme energy and dna methylation level; Influence to elder kidney asthenia person PBL mitochondrial DNA lacks shows and can reduce the mitochondrial disappearance of leucocyte, reduces defective respiratory chain, increases the cell energy needed, keeps cell normal physiological, function in delaying senility thereby reach.

Should act on monarch, minister, help, make principle about Chinese prescription in the modern Chinese medicine theory, in the nutraceutical prescription with anti-oxidation function disclosed by the invention, also embodied, this prescription mainly is to be prepared from according to a certain weight ratio by medicine-food two-purpose remedies post genseng, chrysanthemum, Sessileflower Acanthopanax Bark tea, Poria cocos, Semen sesami nigrum, peach kernel, melissa powder, white fungus, black fungus, Hericium erinaceus, mushroom, grape pip, lycopene, royal jelly etc., is monarch drug in a prescription with stone column genseng, Sessileflower Acanthopanax Bark tea, royal jelly wherein; For strengthening its effect and increase adaptive surface, adopting chrysanthemum, Poria cocos, peach kernel, white fungus, black fungus, grape pip, lycopene again is that minister makes, and strengthens the efficacy of a drug; Assistant is with Semen sesami nigrum, melissa powder, Hericium erinaceus, mushroom etc.Above-mentioned various raw material is through taking ethanol--the active component that extraction with aqueous solution obtains, through experiment confirm repeatedly, medicament selection of the present invention makes up with 14 kinds of medicine-food two-purpose medicines such as stone column genseng and Sessileflower Acanthopanax Bark tea, royal jelly, make each efficacy of drugs produce synergy these drug regimens, thereby can effectively show the effect of the resisting oxidation and delaying senility that it had, effect is fairly obvious and rapid.

The consumption of nutraceutical composition of the present invention, exploring summary in a large number through the inventor draws, the various conventional auxiliary material that each amounts of components needs, be prepared into any peroral dosage form commonly used as disintegrant, lubricant, adhesive etc. with the method for Chinese medicinal of routine, as pill, electuary, tablet, capsule, oral liquid etc.For example these medicine and food raw materials pulverizes can be mixed and make granule and take after mixing it with water; Can also be with these medicine and food raw materials decocting together, condensed water decocting liquid etc. then; But these can not be used to limit protection scope of the present invention.

The specific embodiment

With the routine two parts of test, further set forth effective result of nutraceutical of the present invention by the following examples, these test examples have comprised the function assessment test and the security toxicology test of nutraceutical of the present invention.

Embodiment 1

(1), described nutraceutical, the weight of its preferred medicine and food raw materials respectively is: stone column genseng 1000g, chrysanthemum 1000g, Sessileflower Acanthopanax Bark tea 3000g, Poria cocos 6000g, Semen sesami nigrum 3000g, peach kernel 2000g, melissa powder 3000g, white fungus 2000g, black fungus 2000g, Hericium erinaceus 3000g, mushroom 3000g, grape pip 1000g, lycopene 20g, royal jelly 500g.

(2), preparation method: the preparation method that by specification is introduced carries out.

Embodiment 2,

(1), described nutraceutical, the weight of its preferred medicine and food raw materials respectively is: stone column genseng 2000g, chrysanthemum 2000g, Sessileflower Acanthopanax Bark tea 6000g, Poria cocos 12000g, Semen sesami nigrum 6000g, peach kernel 4000g, melissa powder 6000g, white fungus 4000g, black fungus 4000g, Hericium erinaceus 6000g, mushroom 6000g, grape pip 2000g, lycopene 40g, royal jelly 1000g.

(2), preparation method: the preparation method that by specification is introduced carries out.

Embodiment 3,

(1), described nutraceutical, the weight of its preferred medicine and food raw materials respectively is: stone column genseng 3000g, chrysanthemum 3000g, Sessileflower Acanthopanax Bark tea 9000g, Poria cocos 18000g, Semen sesami nigrum 9000g, peach kernel 6000g, melissa powder 9000g, white fungus 6000g, black fungus 6000g, Hericium erinaceus 9000g, mushroom 9000g, grape pip 3000g, lycopene 60g, royal jelly 1500g

(2), preparation method: the preparation method that by specification is introduced carries out.

The zoopery of the function assessment of test example 1, antioxidation nutritive food

1. pilot project zoopery body weight: lipid peroxide content: MDA or lipofuscin; Antioxidase activity: superoxide dismutase or glutathione peroxidase;

2. test principle

(1) the listed index of zoopery is the project of must surveying.

(2) the optional one of peroxidating operation model animal and aged animal carries out biochemical indicator mensuration.

3. the zoopery method of inspection

(1) animal is selected above aged mouse of 8～12 monthly ages for use, when measuring GSH, SOD index, can select the normal adult animal for use.Single sex, 10～15 every group of mouse, 8～12 of rats.

(2) dosage grouping and be subjected to sample to give the time experiment to establish 3 dosage groups, 1 blank group is one of them dosage group with 5 times of human body recommended amounts, does not establish two dosage groups, the blank group.Given the test agent gives 30 days.

(3) experimental technique peroxidating operation model (D-galactolipin model).

It is excessive that principle: D-galactolipin is supplied with, and extraordinary generation active oxygen has been broken the active oxygen that is controlled by hereditary pattern and produced and the poised state of eliminating, and causes the peroxidating effect.

4. zoopery result judges

Arbitrary index and lipid peroxide content's index are all positive in the activities of antioxidant enzymes, and the anti-oxidant zoopery of this given the test agent of decidable is the positive as a result.

The anti-oxidant zoopery of this nutraceutical the results are shown in Table 1.

This nutraceutical of table 1 is to the influence of the anti-oxidant every index of aged mouse (X ± SD)

* compared significant difference (P＜0.05) with the blank group.

Per os gives this nutraceutical of aged mouse various dose 36d, compare with the blank group, this is tried thing and can reduce myocardium lipofuscin (P＜0.05) in middle dosage group, reduces serum mda content (P＜0.05), improve whole blood glutathione peroxidase activity (P＜0.05); Can reduce myocardium lipofuscin (P＜0.05) in high dose group, reduce serum mda content (P＜0.05), improve whole blood glutathione peroxidase activity (P＜0.05).This is tried thing weight of mice is had no adverse effects.Can judge this nutraceutical tool resisting oxidation and delaying senility effect according to result of the test

The human feeding trial of the function assessment of test example 2, antioxidation nutritive food

1, pilot project

The human feeding trial MDA; Superoxide dismutase; Glutathione peroxidase;

2. test principle

(1) the listed index of human feeding trial is the project of must surveying.

(2) when carrying out human feeding trial, the edible safety of reply given the test agent is done further to observe.

3. the test method and the method for inspection

(1) experimenter includes standard in and selects the age at 45～65 years old, and physical condition is good, does not have obvious brain, the heart, liver, lung, kidney, blood illness, does not have the history of taking medicine for a long time, and aspiration is tried to guarantee the crowd that cooperates.

(2) get rid of experimenter's standard 1. gestation or women breast-feeding their children, to the health food allergy sufferers.2. merge to have the inclination, serious systemic disease patients such as liver, lung, kidney and hemopoietic system.3. take the article relevant in a short time, have influence on judgement person the result with being tried function.

(3) experimenter grouping is divided into test-meal group and control group to the experimenter at random by MDA, SOD, GSH-Px level, considers to influence result's principal element such as age, sex, living and diet custom etc. as far as possible, carries out harmony to check, with the comparativity between the assurance group.Every group of experimenter is no less than 50 examples.

(4) two kinds of control design between test method employing self and group.Test group is subjected to trial product by recommending instructions of taking, dose to take every day, and control group can be with the positive thing with same purpose, also available placebo or negative control.Be no less than 3 months observing time, and duration of test control group and the former life of test-meal group, diet are constant.

(5) the 1. general situation of observation index (safety indexes) comprises spirit, sleep, diet, stool and urine, blood pressure etc.; 2. blood, urine, just routine inspection; 3. liver, kidney function test; 4. Chest X-rays, electrocardiogram, Abdominal B type ultrasonography inspection.

4. the result judges

Each experimental result positive in human feeding trial MDA, superoxide dismutase, three experiments of glutathione peroxidase, this given the test agent of decidable has antioxidation.

The anti-oxidant human experimentation of this nutraceutical the results are shown in Table 1.

This nutraceutical of table 1 is to the influence of the anti-oxidant every index of human body (X ± SD)

* compared significant difference (P＜0.05) with the blank group.

Through this nutraceutical of human feeding trial various dose 36d, compare with the blank group, this is tried thing and can reduce myocardium lipofuscin (P＜0.05) in middle dosage group, reduces serum mda content (P＜0.05), improve whole blood glutathione peroxidase activity (P＜0.05); Can reduce myocardium lipofuscin (P＜0.05) in high dose group, reduce serum mda content (P＜0.05), improve whole blood glutathione peroxidase activity (P＜0.05).This is tried thing body weight gain is had no adverse effects.According to " health food check with assessment technique standard " (2003 editions) to this functional nutrient food oxydating resistance function human trial of criterion decidable of anti-oxidation function health food positive as a result.

30 days feeding trials of security toxicology animal of test example 3, this anti-oxidation function property nutraceutical

1. materials and methods

1.1 sample: this nutraceutical, inspect by ready samples by Beijing Huizhong Mingjie Biotechnology Co., Ltd..Proterties: brown paste.Lot number: 20070421.Proportion: 1.03g/mI.Human body daily intaking amount: 1.12g/d.

1.2 solvent: corn oil.

1.3 animal used as test: 80 of SPF level SD rats, female, hero half and half, body weight 60-80g.Provide by the department of the Chinese Academy of Sciences of Department Of Medicine, Peking University animal used as test section.The animal quality certification number: SCXX (capital) 2006-0008.Animal feeding is in barrier environment.

1.4 animal beverage: provide by Beijing section Austria Beverage Company Limited that pulls together.The feed quality certification number: SCXX (capital) 2005-0007.

1.5 instrument: the full-automatic blood counting instrument of Japanese photoelectricity MEK-6318K, Dutch SELECTRA-E automatic biochemistry analyzer.

1.6 test method: the method according to 30 days feeding trial regulations of Ministry of Public Health's " health food check and assessment technique standard " (version in 2003) is carried out.

1.6.1 grouping: it is negative control group and basic, normal, high three dosage groups that animal is divided into four groups at random by body weight, every group each 20, and male and female half and half.Animal adopts single cage to raise.

1.6.2 filling stomach: sample is a solvent with corn oil, is diluted to desired concn.High, medium and low dosage group is equivalent to 100,50 and 25 times of day for human beings recommended amounts respectively.Promptly 1.87,0.94 and the 0.47g/kg body weight/day.Irritate stomach once at identical time per os every day, irritates stomach amount 0.5ml/100g body weight.Control group gives equivalent corn oil.Irritated stomach weekly 7 days, and increased to adjust according to the weight of animals and irritate stomach amount, continuous irrigation stomach 30 days.Put to death animal the next day of last filling stomach.

1.6.3 observe:

Overview: observe and write down general performance (feed, fur, ight soil proterties and mouth, nose, eye, anus etc. have or not secretion etc.), behavior, poisoning symptom and the death condition of animal every day.Claim body weight weekly one time, the record food intake dose is calculated food utilization.

Routine blood test and biochemical indicator: administration fasting in the 30th day 16 hours, femoral artery is got blood, carries out following inspection: hemoglobin and red blood cell count(RBC), white blood cell count(WBC) and leukocyte differential count and platelet count; ALT (ALT), aspartate amino transferase (AST), urea nitrogen (BUN), creatinine (Crea), blood sugar (GIu), T-CHOL (CHO), triglyceride (TG), total protein (TP) and albumin (ALB) detect.

System dissects and histopathologic examination: take by weighing each treated animal liver, spleen, kidney, sexual gland (testis/ovary), and calculate organ coefficient.Get each treated animal liver, spleen, kidney, stomach and 12 fat intestines, sexual gland (testis/ovary), perusal has or not pathological change.10% formalin fixed, histopathological examination is carried out in conventional H E dyeing.Do not find obvious pathology because of each dosage treated animal being done check substantially, biochemical indicator is not seen obvious change yet, so only carry out the histopathological examination of the main organs of high dose group and control animals.

1.6.4 statistical analysis: each organizes data typing Excel database respectively, calculates mean and standard deviation.Each dosage group and control group compare, and carry out the statistical analysis of difference.

2. result

2.1 overview

This nutraceutical is in 30 days test period, and each dosage group does not all have animal dead, and each treated animal there is no obvious toxic reaction symptom and sign.

2.2 influence to body weight and food utilization

Only first week of body weight of buck relatively is significantly increased with control group this nutraceutical.All the other each each time points of dosage group, the weight of animals and control group no significant difference (seeing Table 1).

This nutraceutical of table 1 is to the influence of rat body weight (X ± s)

Only first week of body weight of buck relatively is significantly increased with control group this nutraceutical.All the other each each time points of dosage group, the weight of animals and control group be no significant difference (seeing Table 2) relatively.Respectively tried treated animal and irritate stomach after 4 weeks, body weight gain, food-intake and total foodstuff utilization rate and control group be no significant difference (seeing Table 3) relatively all.

This nutraceutical of table 2 is to the rat influence of food utilization weekly (X ± s)

^※p<0.05

This nutraceutical of table 3 is to the influence of rat total foodstuff utilization rate (X ± s)

Annotate: total foodstuff utilization rate=(the 4th all body weight-initial body weight) the total food-intake of ÷ * 100%

2.3 influence to hematological indices

Hemoglobin, red blood cell count(RBC) and white blood cell count(WBC), platelet count and the control group of each dosage treated animal of this nutraceutical be no significant difference (seeing Table 4) relatively all.The lymphocyte of each treated animal, neutrophil leucocyte and other percentage of cells and control group be no significant difference (seeing Table 5) relatively all.

This nutraceutical of table 4 is learned the influence (X ± s) of index to rat blood

The influence that this nutraceutical of table 5 is classified to rat leukocyte (X ± s)

2.4 influence to the serum biochemistry index

Reach urea nitrogen and the apparent in view reduction of control group of low and middle dosage group buck in this nutraceutical with the cholesterol of high dose group jenny.All other serum biochemistry indexs are tried relatively all no significant differences (seeing Table 6) of thing group and control group.

This nutraceutical of table 6 is to the influence of rat blood serum biochemical indicator (X ± s)

Compare with control group ^※P＜0.05, ^{※ ※}P＜0.01.

2.5 influence to animal viscera and body weight ratio

Dirty/body ratio of each dosage treated animal of this nutraceutical and the relatively more equal no significant difference (seeing Table 7) of control group.

This nutraceutical of table 7 is to the influence of the dirty body ratio of rat (X ± s)

※ is male to be testis, and female is ovary

2.6 gross anatomy is observed and the result of histopathologic examination

2.6.1 gross anatomy is observed

Each dosage group of this nutraceutical and each internal organs of control group there is no the visible pathological change of tangible naked eyes.

2.6.2 histopathologic examination

Each 20 of this nutraceutical high dose group and control rats are carried out histopathologic examination, the result show liver, spleen, kidney, stomach and duodenum, sexual gland (testis or ovary) histopathologic examination there is no obviously with to being tried the relevant pathological change of thing.

The result of histopathologic examination of high dose group and control animals important organ is as follows:

Liver: the liver cell of high dose group and the female male animal's liver of control group is roughly normal.Each organizes in the lobuli hepatis that all has the part animal a small amount of point-like to occur or the chronic inflammation cellular infiltration of portal area, and pathology is all very light, equal no significant difference between control group and the female male animal of high dose group.Have two animals near the slight steatosis the portal area to occur in female high dose group, owing to pathology only occurs in individual animal, and extent of disease is little, degree is lighter, may be the spontaneous pathology of animal.

Spleen: the spleen white pulp of high dose group and the female tom of control group is normal, and centrum germinativum is normal.Each organizes red pulp, and all as seen expansion is full, and hematopoietic cell as seen.The phenomenon that slight atrophy of spleen white pulp or expansion appear in utmost point individual animal, extramedullary hematopoiesis is active or lower is only arranged, equal no significant difference between control group and the female male animal of high dose group.

Kidney: glomerulus, the renal tubular epithelial of high dose group and the female male animal kidney of control group are not seen obvious pathology.Every group all has a small amount of chronic inflammatory cell infiltration or the expansion of blood vessel extravasated blood in the matter between the individual animal appearance, no significant difference between two groups.

Stomach and 12 batches of intestines: gastric epithelial cell, glandular stomach before the female male animal of high dose group and control group, duodenal mucomembranous epithelial cell is complete, structure is normal substantially.Only have the utmost point individual animal phenomenon of a small amount of inflammatory cell infiltration under stomach or the intestinal mucosa to occur, degree is very light, does not all have significant difference between control group and the female male animal of high dose group.

Testis: the combination of convoluted seminiferous tubule androgone is normal, and the reproduction ripple exists, and interstitial cell is normal.

Ovary: visible each puberty ovarian follicle, corpus luteum exists.

Histopathologic examination's conclusion: the liver,spleen,kidney of this nutraceutical high dose group, stomach, intestines, testis, ovary histopathologic examination, there is no significantly with through the relevant special pathological change of medicine.

3. conclusion:

The result of 30 days feeding trials of this nutraceutical rat shows, by 100 times, 50 times and 25 multiple doses filling stomach of people's recommended intake, every indexs such as animal general behavior sign, body weight gain, food utilization, hematology, blood biochemical, Histopathology are more found with control group and are showed to trying the relevant overt toxicity of thing.So the true border of this nutraceutical is nontoxic.

Claims (8)

1, a kind of nutraceutical with anti-oxidation function and preparation method thereof is mainly made by following medicine and food raw materials in portion by weight: stone column genseng 1-5 part, chrysanthemum 1-5 part, Sessileflower Acanthopanax Bark tea 3-9 part, Poria cocos 3-9 part, Semen sesami nigrum 3-9 part, peach kernel 1-5 part, melissa powder 3-9 part, white fungus 1-5 part, black fungus 1-5 part, Hericium erinaceus 1-5 part, mushroom 2-8 part, grape pip 1-5 part, lycopene 0.02-0.06 part, royal jelly 0.5-1.5 part.

2, nutraceutical according to claim 1, the weight portion of its preferred medicine and food raw materials is: 1 part of stone column genseng, 1 part of chrysanthemum, 3 parts of Sessileflower Acanthopanax Bark tea, 6 parts in Poria cocos, 3 parts of Semen sesami nigrums, 2 parts in peach kernel, 3 parts in melissa powder, 2 parts in white fungus, 2 parts of black fungus, 3 parts of Hericium erinaceus, 3 parts on mushroom, 1 part of grape pip, 0.02 part of lycopene, 0.5 part of royal jelly.

3, the preparation method of nutraceutical according to claim 1 and 2:

(1) take by weighing stone column genseng, Poria cocos etc. 2 flavor medicine and food raw materials in proportion, extract and use 85% alcohol extract, refluxed 3 hours, extract secondary, merging extract vacuum concentrated dry extract 1;

(2) take by weighing Semen sesami nigrum, grape pip, peach kernel etc. 3 flavor medicine and food raw materials in proportion, use 95% alcohol extract, refluxed 3 hours, the extraction secondary, with the extract vacuum concentrated dry extract 2;

(3) take by weighing Sessileflower Acanthopanax Bark tea, Hericium erinaceus, mushroom etc. 3 flavor medicine and food raw materials in proportion, extract, refluxed 3 hours, extract secondary with distilled water, with the extract vacuum concentrated dry extract 3;

(4) take by weighing chrysanthemum, white fungus, black fungus 3 flavor medicine and food raw materials in proportion, use 70% alcohol extract, refluxed 3 hours, extract three times, with the extract vacuum concentrated dry extract 4;

4, merge dry extract 1,2,3,4 it is ground into 100 order powder, remerge melissa powder, lycopene 2 flavor medicine and food raw materials, be ground into 200 order powder jointly, be active component of the present invention.

5, take by weighing the royal jelly medicine and food raw materials in proportion, merge the described active component powder of the present invention of claim 4, after the blending, press the method for production of soft capsule in proportion, be filled into capsule shells.Every soft capsule contains active component 560mg.

6, preparation method according to claim 4, the dry extract that obtains after concentrating also need is following dry 3 hours at 50-60 ℃.

7, the stone column genseng described in this prescription is meant that main product is in China. Kuan Dian stone pillar area, Liaoning Province, the sylvan life ginseng of ginseng order more than life in 15 years.

8, used Sessileflower Acanthopanax Bark tea in this prescription is to cultivate with the patented technology that we have declared, and the stamen leaf of Sessileflower Acanthopanax Bark tea tree is special.