JP6873517B2 - Health-maintaining product composition applicable to adult women, the elderly and people in sub-health conditions - Google Patents

Description

The present invention relates to the fields of foods, supplements, cosmetics and pharmaceuticals, and specifically to a series of health-maintaining product compositions used to improve the sub-health status of adult women and the elderly, or during disease recovery periods.

Menopause in women often develops between the ages of 40 and 60, the main cause of which is the aging of body function, resulting in diminished or diminished ovarian function, and as a result, from the ovaries. It is a series of symptoms caused by a decrease in the amount of estrogen secreted. There are more than 400 types of estrogen receptors in the female body, which are distributed in almost all tissues and organs of the female body. They are controlled and controlled by estrogen (group), and when estrogen decreases, they become the organs of the body. A series of degenerative changes occur in the tissue, resulting in a series of symptoms such as burning, sweating, irritability, agitation, sleeplessness, depression and forgetfulness. Currently, the use of hormone replacement therapy is common for the improvement and treatment of menopause and can temporarily relieve symptoms, but excess or long-term use of hormones is a well-known series of serious cases. It has side effects. Therefore, the sequence caused by "aging" can only be caused by activating the cells of the body, restoring the function of the body, delaying the aging of organs, strengthening the body's immunity and resilience, etc. from the underlying pathology. Physiological or pathological changes, external and internal changes in the body, prevent deterioration of metabolism, fail to achieve a normal balance of endocrine, from dietary nutrition, daily diet Maintain health, maintain youth for a long time, achieve disease prevention, reduction of disease morbidity, reduction or delay of serious illness, improve the level of health and physical resistance of the whole nation.

Aging is a normal phenomenon in nature, and with aging, one's own function declines, physiological and psychological adaptability to the environment and stress ability decline, and body structure and function gradually decline. To go. Biologically, aging is a complex natural phenomenon that manifests itself as degenerative changes in structure and diminished function, and diminished adaptability and resistance. Physiologically, aging is considered to be the developmental course of an individual from a fertilized egg to the elderly. Pathologically, aging is a complex result of stress and fatigue damage, injury and infection, decreased immune response, malnutrition, metabolic disorders, and accumulation of negligence and substance abuse.

There are many theories about the mechanism of aging, but no unified conclusion has been reached, such as immunosuppression theory, neuroendocrine theory, free radical theory, protein synthesis error accumulation theory, gene regulation theory, telomerase theory, etc. Mitochondrial damage theory, autophagy theory, etc. are explained in various literatures and textbooks, and many products are followed up and put on the market. In 2016, American and Japanese scientists (Mills KF et al, Cell Metab. 2016 Dec 13; 24 (6): 795-806) found that 22-month-old mice had β-nicotinamide mononucleotides (NMN). ), We found that the cells and physiological functions of the body were almost the same as those of 6-month-old mice, that is, in the case of humans, NMN performed the functions of the human body from the age of 60 to 20 years. Can be adjusted to the state. This has led to an unprecedented worldwide interest in biological research of NMN, nicotinamide adenine dinucleotide (NAD ⁺ ), and for the past two years, the number one prestigious journals such as Science, Cell and Nature have published. Text on details of related mechanisms [Science. 2018 May 11; 360 (6389); Science. 2017 Sep1; 357 (6354): 928-932; Science.2017 Mar 24; 355 (6331): 1312-1317; Science . 2017 Mar 24; 355 (6331): 1277-1279; Cell. 2018 May 17; 173 (5): 1231-1243; Cell. 2018 Mar 22; 173 (1): 74-89; Cell. 2017 Aug 10; 170 (4): 664-677. Etc.], which indicates that anti-aging theory has ^{entered a new era of NAD +} biology, and many old theories have also been reexamined and reassessed. Has been done. According to this latest theory, NAD ⁺ (Nicotinamide adenine dinucleotide, NAD ⁺ , coenzyme I) plays an important role in metabolic reactions such as energy metabolism, DNA repair, cell survival, and cell cell communication. NAD ⁺ of NMN nicotinamide riboside (NR) is a two intermediates in the biosynthesis effectively increasing the concentration of NAD ⁺ in various tissues, a series of physiological or pathological due to aging and aging Change can be effectively prevented.

NAD ⁺ is a derivative of vitamin PP (nicotinic acid and nicotinamide), NR, and NMN, which is an endogenous substance in the body and a biosynthetic precursor ^{of NAD + in mammals.} Because NAD ⁺ levels decrease in the body system with aging, NAD ⁺ is unstable and ineffective when administered orally, so ^{supplementation with NAD +} synthetic raw material or precursor NMN can be used to supplement the system. By improving the biosynthetic level and capacity of NAD ⁺ , it is possible to maintain the physiological function of mammals and delay the aging process, which is effective in preventing aging and restoring metabolic capacity. Means. Studies have confirmed that long-term oral administration of NMN reaches a safe and acceptable dose of 300 mg / kg and has no significant adverse or toxic effects on normal wild-type c57bl / 6 mice [Stein, LR, and Imai, S. (2014). Specification of Namptin adult neural stem cells recapitulates their functional defects during aging. EMBO J. 33,1321-1340.].

NMN has been reported to be present in a variety of natural foods [Cell Metab. 2016 Dec 13; 24 (6): 795-806] (although there is no specific method or test result for content measurement), for example. , Green soybeans (immature soybeans), broccoli, cucumber seeds, cabbage and other vegetables contain 0.25-1.88 mg / 100 g, and fruits such as avocado and tomatoes contain 0.26-1.60 mg / 100 g. The NMN content in beef and shrimp is relatively low at 0.06-0.42 mg / 100 g. Since the total amount of NMN in human erythrocytes is about 50 mg, various daily diets (eg, diet, medicinal herbs or, etc.) are used to maintain NMN-related amounts that meet the physiological needs of the human body and to make up for deficiencies. Ingested from (health nutritional products), ^{it is beneficial in maintaining the biosynthesis of NAD +} and many physiological functions throughout the body, implementing diets, and achieving the objectives of ensuring longevity and health. As Sun Simiao, who is called the Yakuoh, said, "Doctors first figure out the cause of the illness, discover the physical condition, treat it with a diet first, and if it doesn't work, administer the drug. In this way, most diseases can be cured. Therefore, if you are good at curing, diet is prioritized. "

Haematococcus radicalis extract is extracted from Haematococcus radicalis, and the antioxidant power of astaxanthin contained in it is 550 times that of vitamin E and 10 times that of β-carotene, which is currently the strongest antioxidant.・ It is an ingredient for removing free radicals. The Ministry of Health of the People's Republic of China approved Haematococcus algae as a new food ingredient on 17th 2010. On August 25, 2017, the present inventors obtained a patent right for "a method for rapidly extracting astaxanthin from Haematococcus algae" (ZL 201510764734.1).

Dendrobium candidam is a dried stem of the orchidaceous plant Dendrobium candidam Dendrobium office Kimura et Migo. The reported main components include polysaccharides, flavonoids, etc., which have the effect of improving stomach function, promoting the generation of tsuyu, removing nausea and heat, and due to fever. Lack of fluid, dry mouth, thirst, lack of stomach shadow, loss of appetite, nausea, persistent fever after illness, deficiency and excess heat, bone steaming fever, vision loss, muscle and bone powerlessness Can be used for.

The leaves of Eucommia ulmoides and the seed oil of Eucommia ulmoides are described in Eucommia ulmoides Olive. Oil squeezed from dried leaves and dried ripe fruits. The main active ingredients in the leaves are iridoids, organic acids, amino acids, flavonoids, etc., which have the function of nourishing the liver and kidneys, strengthening muscles and bones, and undernourishing the liver and kidneys, dizziness, and waist. It is useful for maintaining health and treating pain in the knees and muscles and bones. Tochu seed oil is mainly α-linolenic acid (C18: 3) ≧ 45%, oleic acid (C18: 1) ≧ 13%, linoleic acid (C18: 2) ≧ 10%, palmitic acid (C16: 0) ≧ Contains 6%, stearic acid (C18: 0) ≥ 2%. The content of α-linolenic acid in related products of Eucommia seed oil is almost 50% or more, which is the highest in the plant kingdom, and was approved as a new resource food by the Health and Health Commission of the People's Republic of China in 2011 (2011). No. 9 announcement). α-linolenic acid is an essential fatty acid in the human body and can synthesize a series of metabolites such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), stabilizing cell membrane function and maintaining the balance of cytokines and lipoproteins. , Antithrombosis, lowering blood lipids, improving ischemic cardiovascular disease, etc.

Litchi (Ganoderma lucidum) is a dried fruiting body of the red turf Ganoderma lucidum or the purple turf Ganoderma lucidum, which is a fungus of the family Polyporaceae. The main components are triterpenes, polysaccharides, amino acids, proteins, etc., which have the function of replenishing qi, calming nerves, stopping coughing and relieving asthma, and are listed as high quality products in Shennong Ben Cao Jing. There is. The spores are also known as lychee spore powder and have a high immunopotentiating function.

Astragalus mongranaceus (Fish.) Bge. var. mongholicus (Bge.) Hsiao or Astragalus propanaceus (Fish.) Bge. It is a dry root of. Its main components are triterpenoid saponins, polysaccharides and flavonoids, which replenish the qi to raise the sun, protect the skin surface and stop sweating (solid surface antiperspirant), promote urination and eliminate swelling. It has the effects of replenishing tsuyu to nourish the blood, remove congestion and stasis, excrete toxins to remove pus, tighten acne and scars, and promote skin renewal.

Coelorinchus japonicus is a plant of the family Bellflower, Coelorinchus pylosula (Franch.) Nanf. , Coelorinchus coelorinchus codonopsispilosula Nannf. var. Modesuta (Nannf.) L. T. Shen or St. Jin CodonopsistengshenOlive. It is a dry root of. Its main components include saponins, amino acids, proteins, etc., and have the function of strengthening the spleen and lungs, nourishing the blood and generating tsuyu.

Cistanche salsa (desert species) is a plant of the family Orobanche coeruleus, Cistanche deserticola Y. C. Ma's dried fleshy stems with scaly leaves. The main active ingredient is phenylethanoid glycosides such as echinacoside and velvascoside, and its nutritional components are mainly phenylethanoid glycosides, polysaccharides, proteins and amino acids, which make up for the lack of renal yang. It has the effect of supplementing the lack of blood and sperm, moisturizing the intestines and relieving constipation. Its main moisturizing ingredients are betaine and its analogs, which are natural moisturizing factors.

Seisenryu is a plant belonging to the genus Cycrocarya pariurus of the family Walnut family, and is also known as money tree, Mayanagi, and Seisenri. A rare tree species that survived the Quaternary Glacier, endemic to China, found only in China, distributed in many southern provinces, and is now large enough to meet industrial needs. It is artificially cultivated. It is rich in organic nutrients such as saponins, flavonoids and polysaccharides. Seisenryu leaves were approved by the People's Republic of China Health and Health Commission as a new food ingredient in 2013 (Advertisement No. 4, 2013) and can be developed as a tea beverage, containing saponins that are 150 times sweeter than sucrose. Therefore, there are great benefits for diabetics, and there is plenty of evidence that they can cure diabetics.

Yakuo tea is also known as Huaxi Gintan Plum, White Hair Ginro Plum (Potentillagrabra Rodd. Var. Mandshurica (Maxim.) Hand.-Mazz), and is concentrated in the area of the Qinling Mountains of 2200 to 3400 meters. It is often distributed in Mt. Taihaku. Legend has it that when he lived in Mt. Taihaku, he often picked the leaves of "tea maiden" and drank it as tea, and introduced this tea to local residents and Chinese medicine. Later generations call such leaves "Yakuocha". The People's Republic of China Hygiene Bento Food Box [2014] No. 1075 of the People's Republic of China Health Insurance Commission was approved as "ordinary food with traditional eating habits". Since it contains a large amount of strong antioxidant components of polyphenolic acids, it was found that when taken, it has a good effect on improving menstrual irregularities and chloasma in adult women.

Sanyaku (Yamayaku) is a plant of the family Dioscorea family, Dioscorea opposita Thunberg. It is a dry rhizome. It is a traditional yaksik substance. The active ingredient is mainly polysaccharides, and the nutrients are mainly starch and various nutrients, which nourish the spleen and stomach, generate tsuyu to strengthen the function of the lungs, nourish the kidneys and shenkui. It has the effect of stopping nocturnal emission and premature leakage.

Carrot (Ginseng) is a plant of the Araliaceae family, Ginseng Panax ginseng C.I. A. May. Dried roots and rhizomes. It is listed as a new food resource approved by the People's Republic of China. Ingredients are mainly ginsenosides, polysaccharides and proteins with excellent whitening effects that rejuvenate, normalize the pulse and restore the cheerfulness that is about to die, nourish the spleen and lungs, and generate tsuyu. It has functions such as regenerating blood, calming nerves and enhancing intelligence.

Lycium chinense (Lycium chinense) is a plant of the Solanaceae family, Lycium chinense from Ningxia. Dried ripe fruit. It is a traditional yaksik substance. Its constituents include polysaccharides, betaine, carotene, riboflavin, niacin, vitamins B1, B2 and C, and its seeds are rich in various amino acids such as aspartic acid, proline, alanine and leucine. It has the effect of nourishing the liver and kidneys, replenishing sperm and improving vision, and generally causes lack of sperm due to fatigue, pain in the lower back and knees, dizziness, ringing in the ears, impotence, sperm, and inside. Used for rehabilitation and treatment of fever and thirst, blood deficiency and lack of color, and poor vision. "Shennong Ben Cao Jing" states, "If you take wolfberry for a long time, your muscles and bones will become stronger, you will be more resistant to cold and heat, fat accumulation will be prevented, and aging will be delayed. "." Is stated.

Mulberry (mulberry leaf) is a mulberry family plant, mulberry Morus alba L. et al. It is a dried leaf of. It is a traditional yaksik substance. Chemical components include flavonoids, alkaloids, sterols, polysaccharides, etc., which have the effect of expelling wind and heat, cleaning the lungs and moistening the liver, cleaning the liver and improving eyesight. Often used for colds caused by colds, dry cough due to lung heat, dizziness, headaches, red eyes, and diminished vision.

Ceramide is a lipid present in the skin and plays an important role in the formation of the stratum corneum of the epidermis. Skin-beautifying essence cosmetics containing ceramide can enhance the anti-aging function of the skin, keep the skin firm, smooth and delicate, reduce the formation of wrinkles on the face, and in the double layer of biofilm. It is also a degradation product of sphingomyelin and a well-known second messenger, playing a broad and important role in the process of cell growth, proliferation, differentiation, apoptosis and damage.

Sachainchi oil is an oil produced by squeezing the seeds of Sachainchi (Plukenetia volubilis L.) of the Euphorbiaceae family. It mainly contains linolenic acid (C _{18: 3} ) ≧ 35%, linoleic acid (C _{18: 2} ) ≧ 30%, and oleic acid (C _{18: 1} ) ≧ 5%. In 2013, the Health and Health Commission of the People's Republic of China approved it as a new resource food (2013 issue 1 announcement). Its unsaturated fatty acid content is as high as 90% or more, of which the content of α-linolenic acid is 48%, linoleic acid is 35%, and oleic acid is 9%. Alpha-linolenic acid and linoleic acid are also essential fatty acids for the human body and are closely associated with smooth muscle contraction, enzyme activity in lipid metabolism, central nervous system activity, attention and memory enhancement, and other vital functions. doing.

Panax notoginseng (Burk.) F. notoginseng (Burk.), A plant of the Araliaceae family, is known as Panax notoginseng (Burk.). H. It is a dried inflorescence of Chen. Currently, Yunnan is approved as a local special diet. It is rich in ginsenosides and notoginsenosides and has functions such as promoting cell division and growth, increasing cell number, promoting proliferation and release of bone marrow cells, increasing the number and activity of bone marrow erythrocytes, and promoting hematopoiesis.

Burdock kon is a plant of the Asteraceae family, burdock Arctiumlappa L. It is a dried root of sardine and was approved as a general food by the Health Planning Committee of the People's Republic of China in 2013. According to Li Shizhen, "The seedlings are cut, washed to make vegetables, the roots are boiled, and they are dried and stored as dried products, which is beneficial to humans." There is a description that "it improves the condition and removes dirt in the internal organs", and there is a description in "Medicine's separate record" that "when taken for a long period of time, it prevents fat accumulation and prevents aging." In Song and Su Song, there is a description about burdock that "those with large roots are especially beneficial to humans when eaten as vegetables."

Akyo (Ago) is a horse family animal, donkey Equusasinus L. A solid gel made by boiling or condensing dry or fresh rind of the skin, a traditional medicinal and edible substance, with palpitations, hemoptysis, moisturizing and hemostatic functions, mainly blood deficiency. And rehabilitation of blood color deficiency, dizziness, palpitation, muscle atrophy, weakness, irritability, insomnia, vain movement, coughing due to dry lungs, hemoptysis due to overwork, hematemesis, bloody urine, bloody stool / uterine bleeding, and bleeding during pregnancy. Often used for treatment.

Dendrobium candidam, Tianshan snow lotus, 3-carrot (150g of wild carrot), Hyakunishu (120 years of Kashu), Flower shell Bukuryo (60 years of Bukuryo), Miyama Reishi, Undersea pearl, Tochu Kasou, Juyo (Daigei) is known in traditional Chinese medicine as "9 kinds of herbs", and is a Chinese herb that has a specific effect with food by ancient sages by the method of "Shennong tastes 100 kinds of herbs". In the process of discovering, according to various effects and actions, "It has a life-prolonging effect and is not toxic. It is harmless even if it is taken in a large amount for a long period of time. High-quality Chinese medicines that "prevent and prolong life" are further classified, and those with particularly high effects are classified as "9 kinds of herbal medicines".

At present, few of the series of patents containing the above-mentioned medicinal and edible substances are eaten, drunk or taken in combination with NMN, Seisenryu or Yakuohcha. Therefore, we are currently using prior art to combine the relevant development principles of foods, supplements, medicinal herbs, functional foods, specialty foods, cosmetics, pharmaceuticals with the corresponding technical requirements, adult women, the elderly, sub We have developed a series of health-preserving product compositions that can be applied to people in good health.

For the purpose of improving body function in adult women, the elderly and sub-healthy people, the present invention provides a series of health-maintaining product compositions. These compositions are formulated according to the traditional diet theory of Chinese herbal medicine and the latest theory of modern life science to specify the optimum compounding ratio, and different combinations and their compounding ratios cause menopausal symptoms. Improves, restores human cell vitality, regains youth, assists in lowering blood sugar, achieves facial effects, removes chloasma, restores sight and hearing, restores stroke sequelae, of Alzheimer's disease It has effects such as relieving symptoms.

The raw materials according to the present invention are all new varieties of medicinal and edible substances or new food raw materials, and there are no legal barriers to the commercialization of products. That is, the new medicinal and edible substances to be approved include Dendrobium candidum, Reishi, Ougi, Toujin, Cistanchereticola, and Tochu leaves, and the newly approved food ingredients used are mainly , Seisenryu, Yakuohcha, Tochu Seed Oil, Sacha Inchi Oil, Tochu Male Flower, Haematococcus Algae, Nutrients are mainly β-nicotinamide mononucleotide (NMN), Natural Vitamin E, Ceramide, etc. Is included.

In the present invention, valuable anti-aging Chinese herbs such as, for example, dendrobium candidum, sanyaku, reishi, and caterpillar fungus include NMN and its precursors [eg, nicotinamide riboside (NR), nicotinamide (Nam)]. , And NMN analogs (eg, IMP, UMP, etc.) were first discovered to be abundant, the content of which is 0.1-100 mg / 100 g, most of which is 0.2-50 mg / 100 g, and is common. Much more than a variety of vegetables. It not only shows the scientific meaning of "9 kinds of herbs" from the nutritional components, but also Dendrobium candidum (the content of NMN and its precursors and analogues is the highest) is "9 kinds of herbs". Elucidate the true internal cause that is recognized as being. Based on the nutritional properties and efficacy of medicinal and edible substances, a diet that states that "elderly people prefer food to medicine, and the method of treating diseases with food is preferable to the use of medicine". According to the scientific prescription and scientific conclusions drawn from modern pharmacological evaluation techniques, "if the characteristics of foods are understood and used appropriately, they are much more effective than pharmaceutical products." Achieve the purpose of maintaining health and contribute to the development of "healthy Chinese medicine, improving the health level of China".

In the present invention, it has the effects or functions of improving menopausal symptoms, restoring vitality of human cells, regaining youth, achieving facial effects, removing liver spots, etc. Products that can be applied to maintain the health of sub-health test takers are related to various combinations and blending ratios of the following ingredients (including, but not limited to): NMN, Haematococcus algae extract, and ceramide. A combination of various substances (abbreviation A substance) is used alone, or the A substance is the main component and the medicinal and edible Chinese herbal medicine (abbreviation B substance) is the auxiliary component, and the B substance includes dendrobium candidum, sanyaku, and carrot. , Reishi (including lychee spore powder), Yakuocha, Sanshichika, Nikujuyou (desert species), Eucommia leaf, Eucommia seed oil, Eucommia ulmoides, Ougi, Tojin, Kukoshiko, Akyo, etc. Includes combinations of ingredients or combinations with other edible ingredients.

The compounding ratio of substance A is (1-10) :( 1-30) :( 0.1-5), and the amount of substance A used per day is preferably NMN 0.1 mg-500 mg. Haematococcus algae extract 1 mg-1500 mg, ceramide 0.1 mg-300 mg, more preferably NMN 1 mg-300 mg, Haematococcus algae extract 5 mg-500 mg, ceramide 0.2 mg-100 mg.

The amount of the raw material of the B substance used per day (all based on the dry product standard) is preferably 0.1 g-30 g of Dendrobium candidam, 0.1 g-100 g of Sanyaku, 0.1 g-20 g of carrot, and 0. 1 g-20 g, Yakuo tea 0.1 g-10 g, Sanshichika 0.1 g-10 g, Cistanche salsa (desert species) 0.1 g-20 g, and the amount of the remaining components of substance B used is not limited. More preferably, dendrobium candidam 0.5 g-20 g, carrot 0.2 g-10 g, reishi 0.2 g-10 g, Yakuoh tea 0.5 g-5 g, sanshichika 0.3 g-3 g, Nikujuyou (desert species) 0. It is 5 g-10 g, and the amount of the remaining component of the B substance used is not more than three times the amount used by the Pharmacopoeia.

The series of compositions disclosed in the present invention has a clear effect on ameliorating menopause in women, such as burning, sweating, coldness, dizziness, palpitation, irritability, weakness, weakness in the lower back and knees, and insomnia. Menopause with a series of symptoms such as palpitations can be eliminated or alleviated.

The series of compositions disclosed in the present invention has a clear effect on restoring the vitality of human cells and removing chloasma, regaining youth regardless of age, "stopping aging", "restoring youth". , Has effects such as beautiful face and beautiful skin.

Since it has a role of assisting in lowering blood sugar, it can be applied to the health maintenance of diabetes or people who are susceptible to diabetes. The products of the present invention include various combinations and formulations of the following raw materials (including, but not limited to them). Regarding the ratio. A substance, which is a combination of three kinds of substances, NMN, Hematococcus algae extract, and ceramide, is used alone, or the A substance is the main component and the medicinal and edible Chinese herbal medicine (abbreviation C substance) is the auxiliary component, and the C substance. There is one kind of dendrobium kandidam, sanyaku, carrot, oyster, citrus ceramide, eucommia ulmoides, eucommia leaf, eucommia seed oil, eucommia ulmoides, sanshichika, sacha inch oil, gobokon, kukoshiko, soyo It includes a combination of a plurality of types of raw materials or a combination with other edible raw materials (for example, natural vitamin E, vitamin C, etc.).

The requirements for substance A are as described above. The amount of the raw material of the C substance used per day (all based on the dry product standard) is preferably 0.1 g-30 g of dendrobium candidam, 0.1 g-100 g of eucommia ulmoides, 0.1 g-20 g of carrot, and 0. 1g-20g, Cistanche deserticola 0.1g-20g, Eucommia ulmoides 0.1g-50g, Eucommia ulmoides leaves 1g-50g, Eucommia ulmoides seed oil 0.1g-10g, Eucommia ulmoides male flowers 0.1g-20g, Sanshichika 0. It is 1 g-10 g, and the amount of the remaining component of the C substance used is not more than three times the amount used by the Pharmacopoeia. More preferably, Dendrobium candidam 0.5g-15g, Sanyaku 1g-50g, Carrot 0.2g-10g, Ougi 0.5g-10g, Cistanchedeserticola 0.5g-10g, Seisenryu 0.5g-20g, Tochu leaves 2g-20g, Tochu seed oil 0.2g-5g, Tochu male flowers 0.5g-10g, Sanshichika 0.2g-5g, and the remaining amount of C substance used is 2 of the amount used by the pharmacy. It is less than double the amount.

The series of compositions disclosed in the present invention have strong effects such as hypoglycemic activity and antioxidant activity, and are diabetic patients or people who are susceptible to diabetes, women aged 30-60 years in a sub-healthy state, and elderly people aged 60 years or older. It has excellent applicability and health maintenance function for people.

The series of compositions disclosed in the present invention have obvious effects on the metabolic activity of human cells, energy metabolism level, lipid metabolism, recovery of glucose metabolism level, etc., assist in lowering blood glucose, and tolerate the body. It is possible to surely exert the action such as improving the metabolism.

The products of the present invention, which have the effect of restoring the activity and function of nerve cells and can be applied to the elimination of sequelae and health maintenance of stroke patients and the improvement of symptoms of Alzheimer's disease patients, include, but are not limited to, the following raw materials. ) Regarding various combinations and compounding ratios. A substance, which is a combination of three kinds of substances, NMN, Haematococcus algae extract, and ceramide, is used alone, or the A substance is the main component and the medicinal and edible Chinese herbal medicine (abbreviation D substance) is used as the auxiliary component to make the D substance. Is a combination of one or more ingredients such as Cistanche ceramide, dendrobium candidam, carrot, oyster, Eucommia ulmoides seed oil, Eucommia ulmoides, perilla seed oil, Sanshichika, or other edible ingredients (eg, natural). Combination with vitamin E etc.) is included.

The requirements for substance A are as described above. The amount of the raw material of the D substance used per day (all based on the dry matter standard) is preferably 0.1 g-20 g of Cistanche decerticola, 0.1 g-30 g of dendrobium candidum, 0.1 g-20 g of carrot, and the like. Ginseng 0.1g-20g, Eucommia seed oil 0.1g-10g, Eucommia ulmoides 0.1-20g, Ginseng seed oil 0.1-10g, Dendrobium 0.1g-10g, and the remaining components of the D substance. The amount used is less than three times the amount used by the Pharmacopoeia. More preferably, Cistanchedeserticola 0.5 g-10 g, Dendrobium candidam 0.5 g-15 g, carrot 0.2 g-10 g, oyster 0.5 g-10 g, tochu leaf 2 g-20 g, tochu seed oil 0.2 g -5g, male flower of Tochu 0.5g-10g, perilla seed oil 0.2g-5g, Sanshichika 0.2g-5g, and the amount of the remaining components of D substance used is twice the amount used by the pharmacy. It is as follows.

The series of compositions disclosed in the present invention have a clear effect on eliminating the aftereffects of stroke patients, preventing and maintaining the health of people who are susceptible to stroke, and improving the symptoms of patients with Alzheimer's disease. To eliminate or alleviate symptoms such as paralysis, sensory impairment, mouth and tongue distortion, speech impairment, and neuropsychiatric symptoms such as memory impairment, cognitive impairment, personality change and speech impairment in Alzheimer's disease patients or loss of autonomy. it can.

The series of compositions disclosed in the present invention have obvious effects on the regulation of apoptosis and regeneration of nerve cells in the human body, restoration of transmission function between nerve cells, and a series of neuropathy caused by damage caused by removal of free radicals. It is possible to rehabilitate a series of sequelae of stroke patients, prevent and maintain the health of people who are susceptible to stroke, and surely improve the related symptoms of patients with Alzheimer's disease.

The percentage content can be converted to a weight unit known in the pharmaceutical field such as μg, mg, g, kg, or may be a multiple thereof. All of the above weights are based on dry weight or converted to dry weight.

The products of the present invention can be developed in the form of foods, supplements, functional foods, medicinal herbs, special diets, cosmetics, pharmaceuticals, and suitable solid, liquid oral dosage forms, or various product forms for external or cosmetic use.

The problem to be solved by the present invention is the treatment from the etiology, the treatment of both the etiology and the disease, the diet and the nutritional supplementation by the diet, mainly in the form of food, medicine, cosmetics, etc. Menopausal disorders due to decreased ovarian function by meeting the needs for physiological, pathological nutrition or health maintenance, delaying the aging process of the body of adult women and the elderly, increasing body activity, promoting metabolism and energy metabolism By improving the vitality of human cells, regaining youth, achieving facial effects, removing liver spots, etc., and regulating sugar metabolism and lipid metabolism in the human body with medicinal herbs, blood glucose Achieves the effect of supplementarily lowering blood pressure and supplementarily lowering blood pressure, and by utilizing NMN and ceramide, antioxidant by Hematococcus algae, which is a strong natural antioxidant, free radical removal effect, and this nutritional component In combination with edible Chinese herbs containing, it restores the function and apoptosis of nerve cells, thereby restoring sight and hearing, recovering aftereffects of stroke, recovering the symptoms of Alzheimer's disease, etc. Achieve the goal of extending lifespan and maintaining good health through diet. The main idea of the diet by Son Shibaku, who is called Yakuoh, is "The doctor first understands the cause of the illness, discovers the physical condition, treats it with the diet first, and administers the drug if it does not work. However, in this way, most diseases can be cured. Therefore, if you are good at curing, diet is prioritized. ” Contribute to the development of the great health industry.

The series of compositions disclosed in the present invention has a strict formulation of each herbal medicine (or raw material), has excellent synergistic action, has a remarkable effect over a single herbal medicine (or raw material), and all. It is a newly approved food ingredient or a newly approved or already approved ingredient for medicinal and edible substances, has novelty in composition or formulation, and can be safely taken for a long period of time. , No toxicity or side effects. The series of product compositions are orally administered 1-3 times or more per day, preferably once in the morning and evening, or taken for a long period of time according to the product instruction manual.

A series of compositions of all products can be applied mainly to the food sector, and also includes supplements, functional foods, medicinal herbs, specialty foods, cosmetics and pharmaceuticals.

Specifically, the present invention relates to the following contents.
1. 1. (A) β-nicotinamide mononucleotide (NMN) and
(B) Haematococcus algae extract and
(C) Containing ceramide as an ingredient,
A health-preserving product composition that is applicable to adult women, the elderly and people in sub-health conditions.
2. The Haematococcus algae extract contains 3% or more astaxanthin.
Item 2. The composition according to Item 1.
3. 3. The weight ratio (a): (b): (c) of the three components is 0.1-500: 1-1500: 0.1-300.
Item 2. The composition according to Item 1 or 2.
4. The weight ratio (a): (b): (c) of the three components is 1-300: 5-500: 0.2-100.
Item 3. The composition according to Item 3.
5. The weight ratio (a): (b): (c) of the three components is 1-10: 1-30: 0.1-5.
Item 3. The composition according to Item 3.
6. The composition further contains other components having an antioxidant effect, and the other components having an antioxidant effect are one or more selected from dendrobium candidum, Yakuoh tea, Eucommia seed oil, Seisenryu, and Soyo. Is the substance of
Item 8. The composition according to any one of Items 1 to 5.
7. The other component having an antioxidant effect is dendrobium candidam,
Item 6. The composition according to Item 6.
8. The weight ratio of the components (a), (b), and (c) to the dendrobium candidum (a) :( b) :( c): Dendrobium candidum is 0.1-500: 1-500: 0. 1-300: 100-30000, preferably 1-300: 5-100: 0.2-100: 1000-20000.
Item 2. The composition according to Item 7.
9. The other ingredient with antioxidant activity is Yakuo tea.
Item 6. The composition according to Item 6.
10. Weight ratio of Yakuo tea and ingredients (a), (b), (c) (a) :( b) :( c): Yakuo tea is 0.1-500: 1-500: 0.1 −300: 100-10000, preferably 0.1-500: 1-500: 0.1-300: 500-5000.
Item 9. The composition according to Item 9.
11. The composition further contains other components having an anti-fatigue effect, and the other components having an anti-fatigue effect are one or more substances selected from Sanyaku, Litchi, and Cistanche deserticola.
Item 2. The composition according to any one of Items 1 to 10.
12. The composition contains at least one ingredient selected from carrots, sanshichika, akyo, sachainchi oil, bukuryo, ougi, coelorinchus, kukoshi, eucommia ulmoides, natural vitamin E, bilberries, gobokon, perilla seed oil, and male flowers of eucommia ulmoides. ,
Item 2. The composition according to any one of Items 1 to 11.
13. The composition comprises (a) β-nicotinamide mononucleotide, (b) Haematococcus algae extract, (c) ceramide, and tochu seed oil, and sachainchi oil, perilla seed oil as components.
Item 12. The composition according to Item 12.
14. The form of the product composition is selected from foods, cosmetics, and pharmaceuticals.
Item 2. The composition according to any one of Items 1 to 13.
15. The foods are supplements, functional foods, nutritional foods, medicinal foods or special foods.
Item 4. The composition according to Item 14.
16. The product composition is a solid dosage form or a liquid oral dosage form in the case of foods or pharmaceuticals, and the product composition is a solid dosage form or a liquid external dosage form in the case of cosmetics.
Item 4. The composition according to Item 14 or 15.

The following is a specific embodiment of the present invention, further describing the present invention, but does not limit the scope of the present invention. In the following embodiments, the β-nicotine amide mononucleotide is purchased from Wuhan Ensei Co-creation Technology Co., Ltd., and the Eucommia ulmoides extract is prepared and provided by the present inventor in accordance with a registered patent (Zl201510764734.1). Dendrobium Candidum is provided by Zhejiang Iron Kaede Biotechnology Co., Ltd. Imitation Wild Base, Eucommia leaves, Eucommia seed oil, Eucommia ulmoides are provided by Henan Jinduchu Agricultural Technology Co., Ltd. Nikjuyo (Cistanche deserticola) is from the seed planting base of the Naimeng Arazen Alliance, and Yakuocha is made by Jinxi Tai Hakusan Natural Plant Development Co., Ltd. Eucommia ulmoides, natural vitamin E, ceramide, eucommia ulmoides, eucommia ulmoides, sacha inchi oil, and perilla seed oil are available as commercial products.

Important raw material preparation process and quality requirements Explain the composition of subsequent products well, ensure the balance of materials in the production process, and the difference in quality requirements between raw materials and conventional raw materials, and clearly explain the points of invention. To explain the raw material preparation process and its quality.

(1) Dendrobium candidam: Fresh or clean dried products that have been cleaned with foreign substances removed are squeezed or cut into strips and extracted 2-3 times with deionized water (food grade) to extract the extract. It is obtained and concentrated under reduced pressure at low temperature to obtain an extract.
Relative density, water content, total polysaccharide content, and NMN + NR content are measured to provide quality information on raw materials. Quality requirements (invention point): NMN content> 3 mg / 100 g, NR content> 100 mg / 100 g.
(2) Sanyaku: The skinned sunyaku is washed, cut into thin slices or strips, extracted 2-3 times with deionized water (food grade) to obtain an extract, concentrated under reduced pressure at low temperature, and the extract. To get.
Relative density, water content, total polysaccharide content, and NMN + NAD ⁺ content are measured to provide quality information on raw materials. Quality requirements: NMN + NAD ⁺ content (invention point)> 0.1 mg / 100 g.
(3) Carrots: Fresh carrots or carrot drinks are washed and extracted 2-3 times with water (food grade) to obtain an extract and concentrated under reduced pressure at low temperature to obtain an extract.
It measures the relative density, water content, total saponin, and total polysaccharide content, and provides quality information on raw materials. Quality requirements: total saponin content> 2g / 100g, total polysaccharide content> 5g / 100g.
(4) Litchi: Litchi (thin slice) or lychee spore powder is extracted once with edible alcohol to obtain an extract, concentrated under reduced pressure to obtain a concentrated solution 1, and the slag extracted with alcohol is 2-with water. Extract 3 times and concentrate under reduced pressure to obtain a thick paste 2. The concentrate 1 and the thick paste 2 are uniformly mixed and dried at a low temperature to obtain a dry extract, which is pulverized and filled with nitrogen to prepare for use.
It measures the total saponin and total polysaccharide content and provides quality information on raw materials. Quality requirements: total triterpene content> 1g / 100g, total polysaccharide content> 2g / 100g.
(5) Yakuocha: Clean Huaxi Ginro Umeka (Yakuocha) is soaked in pure water at 80-90 ° C for 10-15 minutes 2-3 times, and the dipping solution is combined and depressurized until the appropriate amount is reached. Concentrate and spray dry to obtain a dry powder of water leachate, filled with nitrogen and prepared for use.
It measures the content of polyphenols and provides quality information of raw materials. Quality requirements: Polyphenol content> 5g / 100g.
(6) Seisenryu: Seisenryu is appropriately crushed, soaked in pure water at 80-90 ° C for 10-15 minutes 2-3 times, combined with the dipping solution, concentrated under reduced pressure until an appropriate amount, and dried. Then, a dry powder of the water leachate is obtained and filled with nitrogen to prepare for use.
It measures the total saponin and flavonoid contents and provides quality information of raw materials. Quality requirements: total saponin content> 1g / 100g, total flavonoid content> 2g / 100g.
(7) Sanshichika: Sanshichika is extracted 2-3 times with 50% edible alcohol to obtain an extract, concentrated under reduced pressure to obtain a concentrated solution, dried at a low temperature, dried extract is obtained, and pulverized. , Prepare for use.
It measures the total saponin and flavonoid contents and provides quality information of raw materials. Quality requirements: total saponin content> 5g / 100g, total flavonoid content> 5g / 100g.
(8) Eucommia ulmoides male flowers: Eucommia ulmoides flowers are soaked in water at 80-90 ° C. for 10-15 minutes 2-3 times, combined with the dipping solution, concentrated under reduced pressure at a low temperature until an appropriate amount is obtained, and dried. Obtain dry powder and prepare for use.
It measures the total flavonoid content and provides quality information on raw materials. Quality requirements: Total flavonoid content> 2g / 100g.
(9) Eucommia seed oil: Oil is squeezed from Eucommia seeds by a conventional method, degassed and deodorized to obtain refined oil, or prepared by _{supercritical CO 2 extraction.}
Content requirement: α-linolenic acid> 50%.
(10) Sacha inch oil: Quality requirements α-linolenic acid (C _{18: 3} ) ≧ 35%, linoleic acid (C _{18: 2} ) ≧ 30%, oleic acid (C _{18: 1} ) ≧ 5%.
(11) Haematococcus algae: Prepared according to the registered patent of the present inventors: "Method for rapidly extracting Haematococcus algae extract from Haematococcus algae" (ZL201510764734.1).
(12) Quality of edible grade NMN (β-nicotinamide mononucleotide): Purity of 90% or more, preferably ≧ 95%, more preferably ≧ 98%.
(13) Fujicha: Fujicha (Amperopsis grossedenta) was approved as a tea beverage by the People's Republic of China Health Planning Committee in the announcement of No. 16 of 2013, at 80-90 ° C. Immerse in pure water for 10-15 minutes 2-3 times, combine the dipping solutions, concentrate under reduced pressure to an appropriate amount, and dry to obtain a dry powder, which is ready for use.
(16) Akyo Polypeptide: 1) Remove offensive odors and make it easier to grind: Crush Akyo into small pieces, put in hot roasted clam powder, embed in clam powder, and expose Akyo beads from the clam powder to the surface. Then, it is picked up, and the circular Akyo beads are collected and allowed to cool to prepare for use. 2) The akyo beads from which the offensive odor has been removed are crushed and sieved through an 80 mesh sieve to collect the akyo powder. 3) Akyo powder is enzymatically decomposed with a food enzyme preparation permitted to be used according to the provisions of "Standards for Use of Food Additives" (GB2760-2011), and enzymatically decomposed products (molecular weight <3000 exceeds 80%). Is concentrated and dried to obtain an Akyo active polypeptide powder.

The remaining raw materials are determined by oneself according to the specific requirements of the product, the quality situation of the commercial raw materials and the efficacy at the corresponding quality.

Example Example 1: Tasting study of volunteers to improve menopause Tasting prescription: Produced in a workshop that meets food production hygiene requirements. The ingredients in the tasting formula were all safe edible substances.

Experimental method: Each prescription was grouped into one group, and 30 adult women with menopausal symptoms such as burning, sweating, emotional instability, and memory loss were selected, and the age was 40-55 years and the average age was 50 years. And had them sign a voluntary tasting agreement. For volunteers who orally administer each prescription product twice a day, once each morning and evening, 0.5 g (1 tablet) at a time, administer it continuously for 3 months, and continue to take it voluntarily after 3 months. Followed up for 6 months. The improvement effect of menopausal symptoms was observed by the Kupperman score method, and the status of chloasma and complexion changes was recorded by a self-evaluation method.
Statistical method: All data were analyzed with SPSS 17.0 statistical software, quantitative data results were shown in (x ± s), t-test was used for difference analysis, and the test level was a = 0.05. ..
Experimental results: Thirty adult women took the composition of the present invention for half a month, and menopausal symptoms such as burning, heavy sweating and urinary tract symptoms were clearly alleviated, and after one month, the symptoms disappeared. Almost disappeared or almost disappeared, and after 3 months, the symptoms disappeared completely. After 3 months of use, all the examinees continued to eat voluntarily, and after 6 months, no obvious increase in body weight was observed. Before taking the drug, 30 adult women had a high Kupperman score for menopausal symptoms, while after 6 months of administration, the Kupperman score clearly decreased. Before tasting, about 50% of test takers have chloasma, 90% have pale or bad complexion, and according to test takers, after taking for 3 months, 70% of test takers have liver. The spots were clearly shallower, the complexion became lighter or reddish and moisturized, and after 6 months, 90% of the tasting test takers had all the chloasma disappeared and the complexion was reddish and moisturized. .. It is shown in Table 2.

注：服用前に比べて、＊Ｐ＜０．０５、＊＊Ｐ＜０．０１。

Note: * P <0.05, ** P <0.01 compared to before taking.

Example 2: Medicinal Comparison of Antioxidants of Typical Formulations 2, 5 and 7 Against Mice Experimental Objectives: Glutathione peroxidase (GSH-Px), glutathione-S-transferase (GSH-ST), super in the serum of aged mice By measuring oxide dismutase (SOD) activity, hydrogen peroxide (H ₂ O ₂ ), malondialdehyde (MDA) content, total antioxidant capacity (TAOC) and glutathione reductase activity coefficient (GRAC), respectively. The benefits and effects of prescription anti-aging were compared.
Research target: Typical prescription 2, prescription 5, and prescription 7 with excellent effects were compared and studied, and NMN (prescription 6) was used as a control.
Observational indicators: Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) are important natural antioxidant enzymes in the body that eliminate the accumulation of free radicals and lipid peroxidation of biofilms. It plays a role of inhibiting the destruction of cell structure by free radicals, and is distributed in erythrocytes, serum, liver, brain, etc. Therefore, it should be used as an index to detect anti-aging and protective effects on living organisms. Was done.
Study method: Aged mice induced by D-galactose were randomly divided into a normal control group and an aging model group, and were divided into 200 mg / kg and 400 mg / kg groups for each prescription. After 49d administration, glutathione peroxidase (GSH-Px) in the serum of aged mice, glutathione -S- transferase (GSH-ST), superoxide dismutase (SOD) activity, hydrogen peroxide _{(H 2 O} 2), malondialdehyde The content of (MDA), total antioxidant capacity (TAOC) and glutathione reductase activity coefficient (GRAC) were measured.
Experimental results: Table 3 shows a comparison of serum antioxidant indices in each prescription group. Compared to the normal control group, serum GSH-Px aging model group, T-AOC, SOD, all GSH-ST activity was clearly lowered, whereas, GRAC, the content of MDA and _H 2 _{O 2} are all clearly Rose. Compared aging model group, formulations _groups, H 2 _{O 2} content decreased obviously, T-AOC, SOD, is GSH-ST activity was clearly increased. The effects of Formulations 2, 5 and 7 were better than those of the NMN group, and in particular, Formulations 2 and 7 showed remarkable synergy and the effects were better.

注：正常対照に比べて、＊Ｐ＜０．０５、＊＊Ｐ＜０．０１；老化モデル群に比べて、＃Ｐ＜０．０５、＃＃Ｐ＜０．０１。

Note: * P <0.05, ** P <0.01 compared to normal controls; # P <0.05, ## P <0.01 compared to the aging model group.

Example 3: Intracellular Antioxidant Experiment Objective: A method for measuring intracellular antioxidant activity (CAA) can reflect changes in the absorption, metabolism, and distribution of antioxidants at the cellular level.
^{Test method: HepG2 cells were seeded on a 96-well plate so that the cell density was 6 × 10 4 under} the condition that 100 μL of the culture solution was added per well, and after culturing for 24 hours, the culture solution was removed and the cells were used in PBS. Each seeding well was washed. Then, 100 μL of a sample treatment solution ( ^{containing 25 μmol · L-1} of DCFH-DA) was added to each well, and culturing was continued for 1 h under the conditions of _{37 ° C. and 5% CO 2.} The 96-well board was removed and each well (washed with PBS) was washed with 100 μL PBS, while the 96-well board that duplicated the same test procedure described above was not washed with PBS (washed with PBS). do not). Remove PBS (when washed with PBS) or prescription sample treatment solution (when not washed with PBS ^{), add 100 μL of HBSS (containing 600 μmol L-1} ABAP), and place the 96-well plate in a fluorescent microplate reader. The temperature was maintained at 37 ° C., excited at a wavelength of 538 nm, emitted at a wavelength of 485 nm every 5 min, and measured for 1 h.
After removing the blank and the initial fluorescence value, the area under the time-fluorescence value curve corresponding to the sample mass concentration of each formulation is the CAA value, and the calculation formula is CAA value = 100- (∫SA / ∫CA). It was × 100. In the equation, ∫SA indicates the sample time-integrated area under the fluorescence value curve, and ∫CA indicates the blank control time-integrated area under the fluorescence value curve. The half effect concentration (EC ₅₀ ) of the sample is calculated from the medium effect principle of log (fa / fu) to log (dose), where fa indicates the sample action effect (CAA unit) and fu is 1. -Indicates CAUnit. The EC ₅₀ values are calculated by parallel test three times, and converts an _{EC 50} value in CAA value, indicated by micro molar equivalent corresponding to vitamin C in the sample 100 g. The higher the CAA value of the test material, the better the antioxidant activity.
Experimental Results: The Yakuocha, Haematococcus seed oil, Dendrobium candidum, Seisenryu, and Souyou of the present invention have excellent antioxidant activity, and their strength is equivalent to or slightly lower than that of Vitamin C. Low, Haematococcus algae extract has strong antioxidant capacity and synergistically when used in combination with NMN, NMN-Haematococcus algae-ceramide composition has strong antioxidant capacity, as shown in Table 4. Indicated.

Example 4: Inhibition rate on tyrosinase activity in B16 melanoma cells Objective: Tested the effects of each formulation, such as whitening, and designed and tested this model.
Experimental method: B16 cells of melanoma in the logarithmic growth phase were taken, the cell suspension ^{was adjusted to a cell concentration of 2 × 10 4} cells / mL- ^1, and then seeded in a 96-well culture plate at 180 μL per well. did. A blank control group, a negative control group, and an experimental group were set up in the experiment. When seeded on plates, in the blank control group, only the cell culture medium was added, and in the remaining groups, all cell suspensions were added. Incubate at 5% CO ₂ at 37 ° C. for 24 hours, and after the cells adhere, add 20 μL of different test solution solutions, set up 3 duplications for each concentration, and make 1/10 of the final concentration. It was cultured for 72 hours. The medium was discarded, washed twice with phosphate buffer (PBS), immediately after adding 100 μL of 1% Triton X-100 solution per well, placed in a low temperature refrigerator at −80 ° C. and stored frozen for 30 minutes. The cells were completely ruptured by melting at room temperature. After preheating at 37 ° C., 20 μL of 1% levodopa solution (diluted with PBS, pH <7) was added, and the reaction was carried out at 37 ° C. for 2 hours, and then the absorbance value at 490 nm was measured. Arbutin with a final concentration of 200 μg mL- ¹ was used as a positive control, and the culture medium was used as a negative and blank control.
Preparation of test solution: Using DMSO as a solvent, the NMN concentration was set to 10 μg · mL ^-1 at a ratio of Formulations 2, 5 and 7, respectively, to form a low-dose group, and in the medium-dose group, the NMN concentration was 20 μg · mL ^{−. 1. In} the high dose group, the NMN concentration was controlled to ^{40 μg mL-1.}
Tyrosinase activity inhibition rate = [(average absorbance value of negative control group-average absorbance value of experimental group) ÷ (average absorbance value of negative control group-average absorbance value of blank control group)] × 100

注：ブランク対照に比べて、＊＊Ｐ＜０．０１。

Note: ** P <0.01 compared to blank control.

Experimental results: The results are shown in Table 5. As can be seen from the experiment, the composition of the present invention had an excellent tyrosinase inhibitory activity, and the inhibitory effect increased as the concentration of action increased, showing a certain dose dependence. The strength of tyrosinase inhibitory activity in each group was Formulation 2> Formulation 7> Formulation 5> Arbutin.

Example 5: Mouse Swimming Test In order to compare the anti-fatigue ability of each raw material, a mouse swimming test for determining the formulation was designed.
Experimental method: For the experiment, 1. Control group, 2. Positive control group (American ginseng), 3. The mice were divided into test groups for each raw material, and low-dose group, medium-dose group, and high-dose group were set for each raw material, which was used to measure the swimming time during maximum effort swimming of mice, and 15 mice per group were used. .. Since existing related anti-fatigue drugs are used as raw materials and no horizontal comparison has been made with these drugs, the degree of anti-fatigue strength cannot be evaluated, and the raw materials to be evaluated include Includes Dendrobium Kandydam, Sanyaku, Lychee, Ougi, Cistanche salsa (desert species), NMN, and carrots.
To prepare the crude drug test solution, the mixture was extracted twice with water, the extract was concentrated under reduced pressure, and the mixture was measured up to mark the concentration (g / mL) of each.
Basis for dose design: The medium dose was 100 mg / kg, and the high and low doses were 200 and 50 mg / kg, respectively. The American ginseng positive control group was orally administered at a dose of 100 mg / kg, and the control group was orally administered distilled water. The administration frequency was once / day and the administration time was 4 weeks. For the mice in the 5th group, 30 minutes after the final administration, the ridges of the mice were covered with a lead sheet equivalent to 5% of the body weight and placed in a swimming box (water temperature 25 ° C., water depth 30 cm or more) for swimming. The time from the start of swimming to death was recorded as the swimming time during the maximum effort swimming of the mouse, with the fact that the mouse did not float 10 seconds after sinking to the bottom of the water as a criterion for fatigue death.
Experimental results: The results are shown in Table 6. The most common expression of fatigue is a decrease in exercise endurance, and during a continuous exercise process, increased muscle oxygen consumption, blood glucose and protein consumption cause fatigue, and a decrease in exercise endurance is a decrease in the body's endurance. It was the most direct and objective indicator to reflect the degree of fatigue. Methods for measuring motor endurance include the mouse pole test, mouse tread mill test, and mouse swimming experiment. The mouse pole test is simple, but has a large error, and the mouse that has fallen to the ground is not fatigued. It fell due to factors such as smoothness and differences between the mice. In the mouse swimming experiment, the device is simple, the operation steps are simple, the factors are highly controllable, and the experimental results can intuitively reflect the motor endurance of the body. The longer the swimming time of the mouse during the maximum effort swimming, the more remarkable the fatigue-relieving effect of this raw material was.

As is clear from the above table, ginseng, American ginseng, dendrobium candidum, ougi, cistanche salsa, sanshichika, and NMN all had excellent anti-fatigue effects. The combination of NMN and Haematococcus algae reduced the effect to some extent, but the addition of ceramide clearly increased the effect, indicating that the combination of the three has a good synergistic effect.

Example 6: Effect of NMN and its formulation on rat middle cerebral artery local cerebral ischemia Pharmaceuticals and reagents: NMN content> 95%, Haematococcus algae extract (astaxanthin content 10%), ceramide (food grade, Purity> 95%). Each sample was prepared in the corresponding amount and suspended daily by adding different volumes of pH 6.8 phosphate buffer to the desired concentration prior to administration.
Animals: SD rat, male, body weight 130-150 g, SPF grade.
Experimental method: 60 rats weighing 130-150 g were taken, and depending on the weight, sham surgery group, model control group, NMN group, Haematococcus algae group, NMN-Haematococcus algae group, NMN-Haematococcus algae-ceramide group Randomly divided into 4 groups, 10 animals per group. By gavage, the doses of the NMN group were 50 mg / kg, the doses of the Haematococcus algae group, NMN-Haematococcus algae group and NMN-Haematococcus algae-ceramide group were 40 mg / kg and 50 mg / kg + 40 mg / kg, respectively. The dose was set to 50 mg / kg + 40 mg / kg + 10 mg / kg, all samples were adjusted to appropriate concentrations, and administered continuously for 7 d at a dose volume of about 1 ml / 100 g. An equal volume of saline was administered to the model control group. Rats in each group were anesthetized by intraperitoneal injection of 10% chloral hydrate (350 mg / kg) 1 h after administration on the 6th day. According to the method described in the literature of Tamura et al., The bone was fixed in the right lateral decubitus position, an arc-shaped notch with a length of about 1.5 cm was cut at the midline of the eye angle and the external auditory canal, and the temporal muscle was pinched off and excised. Remove the zygomatic arch with a sculpting forceps to expose most of the squamosal bone, then drill a hole approximately 2 mm below the anterior junction between the zygomatic arch and the squamosal bone with a dental drill and diameter. Formed a small squamosal window of about 2 mm, exposing the middle cerebral artery. 10 L of 50% iron trichloride solution (prepared with 1 mol / L hydrochloric acid) was dropped on a small filter paper, placed in this middle cerebral artery of the rat, left for 30 minutes, the filter paper was removed, and the local tissue was removed with physiological saline. Rinse, sewn layer by layer, general skin disinfection, and then returned to cage for breeding. During the operation, the room temperature was controlled to 23-25 ° C. One more dose was administered 2 days after the operation (23 hours after modeling). After 24 hours of modeling, the head was cut off and the brain was removed.

Experimental results (1)
Effect on neurological symptoms: Rats of each group administered and modeled by the above method were collected, and behavioral scoring of neurological symptoms was performed 6 hours and 24 hours after surgery.
Method 1. Lift the tail of the rat and observe the flexion of the forelimbs, 0 points when the two forelimbs extend symmetrically to the ground, arm flexion, elbow flexion, and internal rotation of the shoulder on the forelimb opposite to the operated side. Alternatively, the cases where both arm-elbow flexion and shoulder internal rotation were observed were scored 1, 2, 3, and 4, respectively. 2. When the animal is placed on a flat surface, each shoulder is pushed to move to the opposite side, the resistance is inspected, 0 points when the resistance from both sides is equal and strong, and when pushing to the side opposite to the operated side When the resistance decreased, it was divided into mild, moderate, and severe according to the degree of decrease, and the points were given as 1, 2, and 3, respectively. 3. 3. The two forelimbs of the animal were placed on a metal mesh and the muscle tensions of the two forelimbs were observed. The case where the muscle tensions of the two forelimbs were equal and strong was 0 points, and similarly, 1, 2 and 3 points were given according to the degree of decrease in limb tension on the side opposite to the operated side. 4. An animal that constantly turns to one side was given 1 point, and according to the score standard, the maximum score was 11, and the higher the score, the more serious the behavioral disorder of the animal.
As can be seen from the results, according to the criteria of the ethological score, different degrees of neurological symptoms were observed in each group of rats after 6 hours and 24 hours of modeling. Compared to the model group, each administration group was able to reduce the behavioral score of cerebral ischemic rats due to mid-cerebral artery embolization to varying degrees, and after 6 hours of modeling, the NMN group, Haematococcus algae group, and NMN-Hemato Comparing the Coccus algae group, NMN-Haematococcus algae-ceramide group and the model group, there was a significant difference, and after 24 hours of modeling, there was a very significant difference, and the NMN-Haematococcus algae group had a very significant difference. The effect of improving neurological symptoms was high, and the NMN-Haematococcus algae-ceramide group had the highest effect of improving neurological symptoms. It is shown in Table 7.

注：モデル群に比べて：＊ｐ ＜０．０１、ＮＭＮ群に比べて：＃ｐ ＜０．０１

Note: Compared to the model group: * p <0.01, compared to the NMN group: # p <0.01

Experimental result (2)
Effect on cerebral infarction range For 6 groups of 10 rats in 1 group, each of which was subjected to behavioral scoring 24 hours after surgery, which was administered and modeled by the above method, the head was cut off and killed, and the entire brain was rapidly removed and the olfactory bulb , The cerebellum and lower brain stem were removed, the left and right brains were separated, each was cut three times in a coronal shape at 0-4 ° C and divided into four pieces, and the brain sections were rapidly stained with TTC (for a dye solution per 5 ml). 4% TTC 1.5 ml, 1 mol / L K ₂ HPO ₄ 0.1 ml) was incubated at 37 ° C. under shading for 30 minutes, then taken out and placed in a 10% formaldehyde solution and stored under shading for 24 hours. When stained, the non-ischemic area was rose red and the infarcted area was white. Carefully remove the white infarct lesion, weigh it, calculate the cerebral infarction range of each animal using the percentage of the weight of the infarcted tissue as the total weight of the brain as the cerebral infarction range, and compare the differences between the groups. did. The left brain was a blank control.
As is clear from the results, the NMN group, Haematococcus algae group, NMN-Haematococcus algae group, and NMN-Haematococcus algae-ceramide group all weigh the infarct range due to middle cerebral artery embolization in rats compared to the model group. The percentage can be clearly reduced and the difference is significant, among which the NMN-Haematococcus algae group has the highest action, indicating that the NMN sum Haematococcus algae extract has a synergistic effect. , The effect was further enhanced by the addition of ceramide. It is shown in Table 8.

注：モデル群に比べて、＊ｐ＜０．０１；ＮＭＮ群に比べて、＃ｐ＜０．０１；ヘマトコッカス藻抽出物群に比べて、★ｐ＜０．０５；ＮＭＮ−ヘマトコッカス藻抽出物群：◆Ｐ＜０．０５。

Note: Compared to the model group, * p <0.01; compared to the NMN group, # p <0.01; compared to the Haematococcus algae extract group, ★ p <0.05; NMN-Haematococcus algae. Extract group: ◆ P <0.05.

Example 7: Effects on climacteric model rats In order to select a pharmacological model that more closely matches the actual physiological and pathological characteristics of climacteric adult women and is closer to clinical symptoms, climacteric model rats were subjected to X-ray modeling methods. A model created and reduced its ovarian function, but not completely eliminated, could more reasonably reflect the characteristics and characteristics of the product.
Animals: 3 month old Wistar female rat, body weight 220 ± 20 g, purchased from Experimental Animal Breeding Center, Animal Research Institute, Chinese Academy of Medical Sciences.
Test drugs: NMN, Haematococcus algae, NMN-Haematococcus algae, NMN-Haematococcus algae-ceramide, estradiol.
experimental method:
(1) Modeling with X-rays: Rats are fixed to a plate, the "ovary region" is exposed, irradiated with an electron linear accelerator at a radiation dose of 5 Gy / min, and high-energy X-rays (6 MeV) are used. Irradiation was performed for 5 minutes, and the total radiation dose was 10 Gy.
(2) Group and dose: 10 animals per group, NMN group, Haematococcus algae group, NMN-Haematococcus algae group, NMN-Haematococcus algae-ceramide, negative control group (irradiation only, no administration) ), A positive drug control group (estradiol valerate tablet), and a blank control group were randomly divided into 7 groups. Dose: NMN group, Haematococcus algae group, NMN-Haematococcus algae group, NMN-Haematococcus algae-ceramide doses are 50 mg / kg, 40 mg / kg, 50 mg + 40 mg / kg, 50 mg + 40 mg + 10 mg / kg, respectively, and all samples. Was dissolved in DMSO to adjust the concentration to an appropriate concentration, and the administration volume was about 1 ml / 100 g, which lasted for 8 weeks.

Experimental result:
(1) General symptoms: After irradiation with X-rays, the rats in each group became less active and inactive, and after 3 days of irradiation, activity began to recover, and after 5 days, activity, feeding, etc. Drinking water became normal, each index of rats in each administration group was normal, and the mental condition was good.
(2) Effect of each group on the body weight of model rats: After modeling, due to the effect of irradiation, the body weights of all the other groups except the blank group decreased to varying degrees. Seven days after modeling, the body weights of each group were all increased to varying degrees and were in good mental condition.
(3) Effect of model rats on follicle-stimulating hormone: After modeling, the follicle-stimulating hormone (FSH) level in each group except the blank group increased sharply, indicating that the modeling was successful, and the negative group after administration. Except for, the FSH levels in each group all decreased, and the estradiol-negative control group did not recover to normal FSH levels after 8 weeks, while the hematococcus algae, NMN group, and NMN + hematococcus algae. The group, NMN-hematococcus algae-ceramide, recovered to normal level at 8, 6, 4, and 2, respectively. From this, Haematococcus algae and NMN have a recovery function against the increase in FSH level of the rat menopausal model, and there is a certain synergistic effect when Haematococcus algae and NMN are used in combination, and NMN-Haematococcus algae-ceramide. It was shown that the synergistic effect in the case of the combined use of was more remarkable. The results are shown in Table 9.

Example 8: Effect of diabetic mouse on blood glucose, insulin and insulin sensitivity index In order to clarify the effect of NMN and its combined formulation on diabetes, the blood glucose, insulin and insulin sensitivity index of diabetic mice were examined and synergistic of the combined formulation. I grasped the action.
Animals: Kunmei mouse, SPF grade, (20 ± 2) g, during the experiment, the breeding environment of the mouse was stabilized, kept at room temperature (25 ± 2) ° C, relative humidity 50% to 70%, and the breeding environment was , Meet the requirements for laboratory animal environmental facilities.
Modeling of diabetic mouse model: 120 mice were taken and acclimatized for 1 week, then 10 mice were randomly selected to be a normal control group, the remaining mice were fasted overnight for 16 hours, and alloxan was injected from the tail vein. Injected at 63 mg · kg ^-1 , and after 72 hours, the fasting blood glucose was measured, and the mouse fasting blood glucose levels> 11.0 mmol · L ^-1 and ≦ 30.0 mmol · L ^-1 were used as criteria for successful modeling. Then, a total of 100 mice satisfy the conditions.
Experimental method: A total of 100 mice with blood glucose levels of 11.0 to 28.5 mmol and L- ¹ were used in the experiment, and model groups, test drug formulations 2, 5, 6 and 7 (200, 400 mg) were used according to the blood glucose level and body weight. The group was randomly divided into a metformin-positive control group (200 mg), and a normal control group was set up to make 10 animals per group, and ig was administered. Physiological saline was orally administered to each of the normal control group and the model group. It was administered continuously for 20 d.
Detection of fasting blood glucose (FBG): 8:00 am fasting, 10:00 administration, 12:00 noon tail vein blood sampling, fasting blood glucose measurement. It was measured once before administration and once every 7 and 14d after administration.

Experimental results: (1) Effect of diabetic mice on fasting blood glucose: In the modeled diabetic mice, the fasting blood glucose clearly increased, and in the metformin group, the hypoglycemic effect gradually increased as the administration time became longer. On the 14th day of administration, the blood glucose decreased significantly (P <0.01) as compared with the model group, and the study drug group tended to lower blood glucose as the usage time became longer, and after administration for 14 days, blood glucose increased. There was a clear decrease and the differences were statistically significant. The hypoglycemic effect is Formulation 7> Formulation 2> Formulation 6 ≒ Formulation 5, which indicates that NMN has an effect of auxiliary lowering blood glucose, and when ceramide is added, a clear synergistic effect is obtained. there were. It is shown in Table 10.

注：正常対照群に比べて、１）Ｐ＜０．０５、２）Ｐ＜０．０１；モデル群に比べて、３）Ｐ＜０．０５、４）Ｐ＜０．０１

Note: Compared to the normal control group, 1) P <0.05, 2) P <0.01; compared to the model group, 3) P <0.05, 4) P <0.01

Experimental Results: (2) Effects of Diabetic Mice on Insulin and Its Sensitivity Index (ISI) Levels The model group of mice had significantly lower serum insulin than that of the normal control group. Compared to the model group, the mice in the study drug group and the metformin group had elevated serum insulin levels, which showed that they had an insulin secretagogue action, and the effect was prescription 7> prescription 2> prescription 6 ≒ prescription 5. From this, it was shown that NMN has an insulin secretagogue action, and when combined with ceramide, there was a synergistic action. It is shown in Table 11.

注：正常対照群に比べて、１）Ｐ＜０．０１；モデル群に比べて、２）Ｐ＜０．０５、３）Ｐ＜０．０１

Note: Compared to the normal control group, 1) P <0.01; compared to the model group, 2) P <0.05, 3) P <0.01

Example 9: Effect of diabetic mouse on fasting blood glucose Experimental animal: Kunming mouse, SPF grade, body weight (20 ± 2) g, half each of male and female, temperature controlled to (20 ± 2) ° C, light-dark cycle The mice were bred in an environment of 12 hours each and allowed to drink water freely.
Test drug: NMN, Haematococcus algae, NMN-Haematococcus algae, NMN-Haematococcus algae + ceramide group, metformin hydrochloride tablets (manufactured by Shanghai Shinryo Yakuhin Co., Ltd.).
Experimental method: 70 mice, half of each male and female, were subjected to blank control group, model control group, positive control group (methformin hydrochloride tablets, 200 mg / kg, equivalent to 10 times the clinical dose), NMN, depending on gender and body weight. The group was randomly divided into 7 groups: Haematococcus algae group, NMN-Haematococcus algae group, and NMN-Haematococcus algae + ceramide group, and the doses were 200 mg / kg, 150 mg / kg, 200 mg + 150 mg / kg, 200 mg + 150 mg + 10 mg / kg, respectively. There were 10 animals per group, and they were administered by gavage at a body weight volume of 20 mL / kg. In the blank control group and the model control group, an equal amount of physiological saline was orally administered once / d for 20 consecutive days, and on the 7th day, the animals in each group were fasted or non-water-free. After 24 hours, all the mice in the remaining groups except the blank control group were injected with 200 mg / kg of Aloxane (dissolved in 0.9% saline) in a lump, and the mice in the blank control group were injected. Equal volume of saline is injected, 4.5-5.0 hours later, 50% GS 0.5 mL / animal ig is administered to each group of mice, and 72 hours later, each group of mice is banned. Eating and non-watering were performed for 12 hours, the end of the tail was cut off, blood was collected, and the blood glucose concentration of the mice in each group was detected by a glucose meter. After administration for up to 20 days, the mice in each group were fasted and non-watered for 12 hours, the end of the tail was cut off and blood was collected, and the blood glucose concentration of the mice in each group was detected with a glucose meter. did.

Experimental results: As shown in Table 12, the mice in each dose group, except for the Haematococcus algae group, had a clear decrease in blood glucose on days 10 and 20, showing a difference compared to the model control group. It was statistically significant, NMN had a certain hypoglycemic effect, and when combined with ceramide, it had a significant synergistic hypoglycemic effect, and Haematococcus algae functioned as a stabilizer.

注：正常対照群に比べて、＊Ｐ＜０．０１；モデル対照群に比べて、＃Ｐ＜０．０１。

Note: * P <0.01 compared to the normal control group; # P <0.01 compared to the model control group.

Example 10: Analysis of NMN content in precious Chinese herbs In order to study whether or not precious Chinese herbs contain NMN, LC-MS (Liquid Mass Spectrometry) technology was used to control which NMN control product was used as a control. After studying whether NMN is contained in Chinese herbs, it is the first time that valuable Chinese herbs such as Tochu Kasou, Dendrobium huoshanense, Sanyaku, and Dendrobium Kandidam all contain NMN and its similar substances. It's been found. In addition, a method for measuring the content of such a component has been invented, and from the measurement results, the content in dendrobium candidam was the highest.
Experimental method: Tochu Kasou, Dendrobium fuoshanense, Sanyaku, Dendrobium candidam crushed and sieved to 50 mesh sieve (No. 3 sieve), take 20 g of each, add 20 ml of deionized water to it, and use ultrasonic waves for 30 min. Extracted and filtered, the supernatant was taken, quantified by LC / MS / MS, the concentration of the NMN control product (purchased from Wuhan Ensei Biotechnology Co., Ltd.) was 0.4 mg / ml, and the liquid phase conditions were: (A: water + 0.1% formic acid, B: acetonitrile + 0.1% formic acid), and is shown in Table 13.

Experimental results: The content of NMN in 100 g of precious Chinese herbal medicine was 9.4 mg of Tochukasou, 1.1 mg of Dendrobium fuoshanense, 1.1 mg of Sanyaku, and 30.6 mg of Dendrobium candidum (a variety similar to wild species), respectively. .. Dendrobium candidum (a variety similar to wild species, dry product equivalent) also has similar nucleoside (nucleotide) components, such as NR (nicotinamide riboside) 125.27 mg / 100 g, AMP (adenylic acid) 0. 93 mg / 100 g, UMP (uridylic acid) 0.48 mg / 100 g, and IMP (inosinic acid) 0.23 mg / 100 g were contained. Furthermore, for Sanyaku (iron stick yam), NAD ⁺ (nicotinamide adenine dinucleotide) 0.16 mg / 100 g, NR (nicotinamide riboside) 6.45 mg / 100 g, Nam (nicotinamide) 0.38 mg / 100 g, AMP (adenylic acid) 0.38 mg / 100 g and UMP (uridylic acid) 0.24 mg / 100 g were contained at the same time. For these Chinese herbs, it was the first time to identify the above-mentioned multiple nutritional components and to discover and quantify their contents.

Example 11: Preparation method of a typical product for alleviating climacteric symptoms NMN, haematococcus algae extract, ceramide, dendrobium candidum, sanyaku, carrot, lychee (lychee spore powder) are important raw material varieties of this product. (Including), Yakuocha, Sanshichika, Nikujuyou (desert species), Tochu leaf, Tochu seed oil, Tochu male flower, Ougi, Tojin, Kukoshiko, Akyo, etc. In combination with edible ingredients, the most suitable formulation was selected according to the efficacy and prepared into the appropriate dosage form and product. The method for preparing each raw material is described in the "Important raw material preparation process and quality requirements" section of the above embodiment.
Examples of single dose prescriptions (tablets, granules, bags, etc.) are as follows.
Life seed formulation: NMN 50 mg, Haematococcus algae extract 200 mg, ceramide 10 mg, and an appropriate amount of auxiliary material were prepared in an appropriate dosage form.
Reproduction of Shusen: Dendrobium candidum extract 120 mg, NMN 20 mg, Haematococcus algae extract 100 mg, ceramide 10 mg, and an appropriate amount of auxiliary material were prepared in an appropriate dosage form.
Lychee prescription (lychee dendrobium candidum carrot complex prescription): 200 mg of dendrobium candidum extract, 50 mg of lychee extract, 10 mg of carrot extract, 20 mg of NMN, and 50 mg of Yakuo tea water extract were prepared in a beverage.
Menopausal prescription (prescription for relieving menopausal disorders): Dendrobium candidum extract 200 mg, Eucommia ulmoides flower extract 100 mg, Cistanche salsa (desert species) extract 100 mg, NMN 30 mg, Akyo polypeptide 50 mg are prepared in a beverage. did.
Non-re-prescription for menopause (prescription for eliminating menopause): Sanyaku extract 200 mg, Ougi extract 100 mg, Tochu leaf extract 200 mg, Nikujuyo (desert species) extract 100 mg, Tojin extract 50 mg, Kukoshiko extract 50 mg , NMN 30 mg, Sanshichika water extract 10 mg were prepared into a solid beverage.
Popular energetic formulations: 100 mg of lychee spores, 100 mg of Eucommia seed oil, 40 mg of NMN, and 60 mg of Haematococcus algae extract were prepared in soft capsules.
Using the raw materials of the above formulation, corresponding dosage forms and products were prepared under the conditions satisfying the food hygiene requirement or the GMP requirement. The main dosage forms include tablets (candy tablets), capsules (soft, hard), emulsions, beverages (liquid or solid form), concentrates (pastes), cosmetics (masks, creams, emulsions, essences), etc. ..

Example 12: Preparation method of a typical product for youth retention / rejuvenation The main raw material varieties of this product are Nikujuyo (desert species), Dendrobium candidam, Sanyaku, NMN, and Haematococcus algae extraction. Includes products, ceramides, oligopeptides 1, EGF, carrots, akyo polypeptides, dendrobium, Yakuo tea, sacha inchi oil, wisteria tea and the like. The optimal formulation was selected according to the efficacy and prepared into the appropriate dosage form and product. The method for preparing each raw material is described in the "Important raw material preparation process and quality requirements" section of the above embodiment.
Examples of single dose (tablets, granules, bags, etc.) formulations are as follows.
Rejuvenation prescription: NMN 60 mg, ceramide 10 mg, oligopeptide 1 0.1 mg, EGF 0.1 mg, Akyo polypeptide 20 mg, and an appropriate amount of auxiliary material were prepared in an appropriate dosage form.
Youth retention formulation: Dendrobium candidum extract 150 mg, Sanyaku extract 100 mg, NMN 20 mg, Haematococcus algae extract 100 mg, Ceramide 10 mg, Wisteria tea water extract 20 mg, Sanshichika extract 10 mg, Yakuo tea 40 mg, Akyo polypeptide 50 mg , Auxiliary material The appropriate amount was prepared in the appropriate dosage form.
Litchi spore oil 100 mg, ginsenoside 30 mg, NMN 40 mg, Haematococcus algae extract 150 mg, and Sachainch oil 130 mg were prepared into soft capsules or microemulsions.
Using the raw materials of the above formulation, the corresponding dosage forms and products were prepared under the conditions satisfying the food or cosmetic hygiene requirements or the supplement GMP requirements. The main dosage forms include tablets (candy tablets), capsules (soft, hard), emulsions, beverages (liquid or solid form), concentrates (pastes), cosmetics (masks, creams, emulsions, essences), etc. ..

Example 13: Method for preparing a typical product for improving the symptoms of Alzheimer's disease The main raw material varieties of this product are lychee (desert species), dendrobium candidum, carrot, litchi, lychee, NMN, and haematococcus algae. Extracts, ceramides, male flowers of litchi, tochu leaves, lychee spore oil, tochu seed oil, perilla seed oil, lychee (including lychee spore powder), lychee, etc., or other edible ingredients (eg, natural vitamin E, etc.) ) Is included. The optimal formulation was selected according to the efficacy and prepared into the appropriate dosage form and product. The method for preparing each raw material is described in the "Important raw material preparation process and quality requirements" section of the above embodiment.
Examples of single dose (tablets, granules, bags, etc.) formulations are as follows.
Intellectual improvement prescription for the elderly: Sanshichika extract 20 mg, NMN 50 mg, ceramide 10 mg, Ougi extract 90 mg, carrot extract 30 mg, Bukuryo extract 50 mg, and an appropriate amount of auxiliary material were prepared in an appropriate dosage form.
Intellectual improvement formulation: Dendrobium candidum extract 150 mg, carrot extract 10 mg, NMN 50 mg, Haematococcus algae extract 100 mg, ceramide 15 mg, Tochu leaf water extract 100 mg, and an appropriate amount of auxiliary material were prepared in an appropriate dosage form.
Bagging agent: 100 mg of lychee spore oil, 50 mg of ginsenoside, 60 mg of NMN, 150 mg of Haematococcus algae extract, 100 mg of tochu seed oil, and 40 mg of perilla seed oil were prepared into soft capsules or microemulsions.
Using the above ingredients as in the formulation, they were prepared into the corresponding dosage forms and products under conditions that meet food hygiene requirements or supplement GMP requirements. The main dosage forms include tablets, capsules (soft, hard), emulsions, beverages, concentrates, cosmetics (masks, creams, emulsions, essences) and the like.

Example 14: Method for preparing a typical product for the recovery period after stroke The main raw material varieties of this product are Sanshichika, carrot, Ougi, NMN, Haematococcus algae extract, ceramide, male flower of Tochu, and Reishi spore. Includes oil, tochu seed oil, sacha inchi oil, etc., or in combination with other edible ingredients. The optimal formulation was selected according to the efficacy and prepared into the appropriate dosage form and product. The method for preparing each raw material is described in the "Important raw material preparation process and quality requirements" section of the above embodiment.
Examples of single dose (tablets, granules, bags, etc.) formulations are as follows.
Zhaofu self-safety prescription (stroke improvement prescription): NMN 60 mg, Haematococcus algae extract 200 mg, ceramide 10 mg, Sanshichika extract 30 mg, Tochu male pollen extract 100 mg, Ougi extract 100 mg, appropriate amount of auxiliary material, appropriate agent Prepared in shape.
Wind-stop prescription (stroke prevention prescription): 100 mg of lychee spore oil, 50 mg of ginsenoside, 60 mg of NMN, 150 mg of haematococcus algae extract, 100 mg of tochu seed oil, and 40 mg of sachainchi oil were prepared into soft capsules or microemulsions.
Using the above ingredients as in the formulation, they were prepared into the corresponding dosage forms and products under conditions that meet food hygiene requirements or supplement GMP requirements. The main dosage forms include tablets, capsules (soft, hard), emulsions, beverages, concentrates, cosmetics (masks, creams, emulsions, essences) and the like.

Example 15: Method for preparing a typical product for supplementarily lowering blood glucose The main raw material varieties of this product are spores, sanshichika, gobokon, soybean, ougi, dendrobium candidum flower, and dendrobium candidum. , Kukoshi, NMN, Haematococcus algae extract, male flower of Tochu, Reishi spore oil, Tochu seed oil and the like. The optimal formulation was selected according to the efficacy and prepared into the appropriate dosage form and product. The method for preparing each raw material is described in the "Important raw material preparation process and quality requirements" section of the above embodiment.
Examples of single dose (tablets, granules, bags, etc.) formulations are as follows.
Tang dynasty prescription (prescription for suppressing increase in blood glucose): NMN 80 mg, Haematococcus algae extract 400 mg, ceramide 20 mg, and an appropriate amount of auxiliary material were prepared in an appropriate dosage form.
Sugar friend prescription (prescription for diabetic patients): Dendrobium candidum (or flower) water extract 100 mg, Seisenryu water extract 150 mg, NMN 50 mg, Haematococcus algae extract 100 mg, Kukoshi extract 50 mg, Gobokon extract 50 mg, ceramide 50 mg, an appropriate amount of auxiliary material, was prepared in an appropriate dosage form.
Eucommia ulmoides prescription: 150 mg of saponin derived from Eucommia ulmoides, 50 mg of Eucommia seed oil, 50 mg of male pollen extract of Eucommia ulmoides, 100 mg of Reishi spores, 50 mg of NMN, and 100 mg of Haematococcus algae extract were prepared into soft capsules or emulsions.
Using the above ingredients as in the formulation, they were prepared into the corresponding dosage forms and products under conditions that meet food hygiene requirements or supplement GMP requirements. The main dosage forms include tablets, capsules (soft, hard), emulsions, beverages, concentrates, cosmetics (masks, creams, emulsions, essences) and the like.

Claims (12)

(A) β-nicotinamide mononucleotide (NMN) and
(B) Haematococcus algae extract and
(C) With ceramide
As an ingredient ,
In addition, it contains dendrobium candidum as another component with antioxidant activity.
Health holding product composition characterized by. The Haematococcus algae extract contains 3% or more astaxanthin.
The composition according to claim 1. The weight ratio (a): (b): (c) of the three components is 0.1-500: 1-1500: 0.1-300.
The composition according to claim 1 or 2. The weight ratio (a): (b): (c) of the three components is 1-300: 5-500: 0.2-100.
The composition according to claim 3. The weight ratio (a): (b): (c) of the three components is 1-10: 1-30: 0.1-5.
The composition according to claim 3. The weight ratio of the components (a), (b), and (c) to the dendrobium candidum (a) :( b) :( c): Dendrobium candidum is 0.1-500: 1-500: 0. 1-300: 100-3000 0 ,
The composition according to any one of claims 1 to 5, wherein the composition is characterized by the above. The composition further contains other components having an anti-fatigue effect, and the other components having an anti-fatigue effect are one or more substances selected from Sanyaku, Litchi, and Cistanche deserticola.
The composition according to any one of claims 1 to 6 , wherein the composition is characterized by the above. The composition contains at least one ingredient selected from carrots, sanshichika, akyo, sachainchi oil, bukuryo, ougi, coelorinchus, kukoshi, eucommia ulmoides, natural vitamin E, bilberries, gobokon, perilla seed oil, and male flowers of eucommia ulmoides. ,
The composition according to any one of claims 1 to 7 , wherein the composition is characterized by the above. The composition comprises as component preparative Chu seed oil, Sacha inch oil and perilla seed oil,
The composition according to claim 8. The form of the product composition is selected from foods, cosmetics, and pharmaceuticals.
The composition according to any one of claims 1 to 9 , wherein the composition is characterized by the above. The foods are supplements, functional foods, nutritional foods, medicinal foods or special foods.
The composition according to claim 10. The product composition is a solid dosage form or a liquid oral dosage form in the case of foods or pharmaceuticals, and the product composition is a solid dosage form or a liquid external dosage form in the case of cosmetics.
The composition according to claim 10 or 11.