KR20140036966A - Composition for improving exercise capacity or a composition for reducing fatigue using leaves of sasa quelpaertensis - Google Patents

Abstract

The present invention discloses a composition for improving exercise performance and fatigue using the jejudae leaves. Specifically, the present invention provides free radical scavenging ability, nitric oxide (NO) scavenging activity through DPPH experiment, and high vaginal activity, endurance, anti-fatigue activity, valine, leucine, and isoleucine with high induction activity of antioxidant enzyme HO-1. , Amino acid such as arginine, methionine, aspartic acid, and selenium related to improving immune function and muscle function, etc. Sasa quelpaertensis leaves containing higher content than Sasa borealis Disclosed are compositions for improvement and fatigue recovery.

Description

Composition for improving exercise capacity or a composition for reducing fatigue using leaves of Sasa quelpaertensis}

The present invention relates to a method for producing Sasa quelpaertensis ) relates to a composition for improving exercise performance and fatigue using leaves.

Fatigue is defined as a state in which physical and mental activity ability is reduced, and physical fatigue (meaning a state in which exercise performance is reduced) (Establishment of functional evaluation system related to fatigue recovery of health functional food, 2004 Ministry of Food and Drug Safety Research Report) is caused by the accumulation of fatigue substances such as lactic acid in muscles (Fitts et al., 1976; Savitski et al., 1979).

In order to relieve physical fatigue, the market for products that suppress the accumulation of fatigue substances in the body and the functional product market focused on improving exercise performance are being activated, but products containing compounds such as steroids, caffeine, sodium bicarbonate and sodium citrate While taking a certain amount of silver can significantly increase exercise performance, the drug carries a risk of fatal side effects and ultimately health. Therefore, in recent years, research for developing products for improving fatigue and improving exercise performance using natural products, such as plant extracts, has been actively conducted.

Among the amino acid components, valine, leucine, and isoleucine, also called BCAA (Branched-Chain Amion Acid), are important amino acids for building muscle. This BCAA amino acid has the effect of improving muscle strength, quickness, endurance, elasticity, etc., and it effectively removes lactic acid, which is a fatigue material produced by exercise, and also helps to recover from fatigue after exercise (Wagenmakers, 1992; Davis, 1995). . In addition, anti-fatigue effects of arginine, methionine and aspartic acid are known (Pratusevich and Alekseeva, 1971; Guarnieri et al., 1979; Meneguello et al., 2003; Abel et al., 2005). Development of functional products containing amino acids for improving performance is continuously being attempted.

Selenium is one of the essential nutrients. It has antioxidant properties, improves sexual function, and has an excellent effect on heavy metal detoxification. In addition, it is effective in preventing liver cirrhosis by preventing necrosis of liver cells, and has been proven to be effective in helping to prevent immune diseases, improve lung and heart function, and prevent heart disease. Deficiency of selenium can cause muscle function problems, which can lead to muscle pain, muscle exhaustion, work experience, and cardiomyopathy. Creatine kinase (CK), an indicator of muscle damage, increases blood levels during fatigue, even in the absence of selenium (Chariot and Bignani, 2003; Rannem et al., 1995). However, since selenium is not supplied in a sufficient amount through a daily meal, there is a need for a method of effectively ingesting selenium through a daily food.

When the living body becomes tired due to physical and mental stress, the function of the pituitary-adrenal system (HPA) is enhanced and the hormones related to stress are secreted, as well as enhancing the energy metabolism in vivo to prepare for the next stress. This results in increased oxygen consumption (Kzennet et al., 1985). In the case of physical stress, the increased oxygen consumption occurs according to muscle energy metabolism requirements. Oxygen is essential for organisms with organic respiration, but is incompletely reduced during energy metabolism to generate reactive oxygen species (ROS). Reactive oxygen species are highly reactive and oxidize important constituents in cells such as proteins, DNA and lipids, destroying the homeostasis of cells and leading to death in the past, and the accumulation of such cell damage causes aging and various degenerative diseases. Known as Continued exercise and the resulting increase in energy metabolism promote the generation of reactive oxygen species. Antioxidant systems for suppressing the toxicity of these generated reactive oxygen species are the major defense mechanisms to suppress damage to the body and continue to perform exercise and fatigue recovery. Essential (Show et al., 1992; Halliwell et al., 1984; Gutteridge et al., 1995; Møller et al., 1996).

Sagittarius ( Sasa) borealis ) is a herbaceous plant belonging to the genus of rice plants. It is an evergreen plant that grows in a group under a large tree under the mountainside in central Korea. It is classified as an indigenous herb. It is also called by other names such as mountain bamboo, jijuk (地 竹), jojuk (鳥 竹), and japuk (立 竹). Both leaves, stems, and roots are used as medicine, and any flowers that are not flowering can be harvested and used at any time. The nature of stalks is known to be cold, slightly sweet, alkaline and non-toxic. In oriental medicine, it has been used as a folk remedy because it has antipyretic, sodium, sodam, tonic, antibacterial and anticancer effects. It has been experimentally demonstrated that hot water and 70% ethanol crude extracts of saponica leaves exhibit antioxidant activity (Park et al., 2009). It was also confirmed that the extracts of the leaves and stems showed good antibacterial activity against food poisoning and decaying bacteria (Ko., 2008).

Especially Jeju Sasadae quelpaertensis ) is distributed over Hallasan, Jeju Island, and has white bands on the edges of leaves. Branches do not split, are short, and the shape of the node is different from that of inland. The functionality of Jeju jejudae began to be studied in the late 2000s, and it has been shown to help whitening, anti-obesity, anti-inflammatory, anti-diabetic, and balance of electrolytes and water in the body (An et al., 2008) Kang et al., 2012; Moon et al., 2011; Ryou et al., 2012).

[Reference literature]

Abel T, Knechtle B, Perret C, Eser P, von Arx P, Knecht H. Influence of chronic supplementation of arginine aspartate in endurance athletes on performance and substrate metabolism-a randomized, double-blind, placebo-controlled study. Int J Sports Med. 2005 Jun; 26 (5): 344-9.

An SM, Lee SI, Choi SW, Moon SW, Boo YC. p-Coumaric acid, a constituent of Sasa quelpaertensis Nakai, inhibits cellular melanogenesis stimulated by alpha-melanocyte stimulating hormone. Br J Dermatol. 2008 Aug; 159 (2): 292-9.

Chariot P, Bignani O. Skeletal muscle disorders associated with selenium deficiency in humans. Muscle Nerve. 2003 Jun; 27 (6): 662-8.

Davis JM. Central and peripheral factors in fatigue. J Sports Sci. 1995 Summer; 13 Spec No: S49-53.

Fitts RH, Holloszy JO. Lactate and contractile force in frog muscle during development of fatigue and recovery. Am J Physiol. 1976 Aug; 231 (2): 430-3.

Guarnieri C, Fussi F, Fanelli O, Davalli P, Cl C. RNA and protein synthesis in rat brain during exercise. Effect of arginine and some phosphorylated amino acids. Pharmacology. 1979; 19 (1): 51-6.

Gutteridge JM. Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem. 1995 Dec; 41 (12 Pt 2): 1819-28.

Halliwell B, Gutteridge JM. Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J. 1984 Apr 1; 219 (1): 1-14.

Kang SI, Shin HS, Kim HM, Hong YS, Yoon SA, Kang SW, Kim JH, Ko HC, Kim SJ. Anti-obesity properties of a Sasa quelpaertensis extract in high-fat diet-induced obese mice. Biosci Biotechnol Biochem. 2012; 76 (4): 755-61. Epub 2012 Apr 7.

Kennett GA, Dickinson SL, Curzon G. Enhancement of some 5-HT-dependent behavioural responses following repeated immobilization in rats. Brain Res. 1985 Mar 25; 330 (2): 253-63.

Ko MS. Chemical components in stalks and leaves of Sasa borealis makino and antioxidative and antimicrobial activities of extracts. Korean J Food Preserv 2008 15: 125-132.

Meneguello MO, Mendona JR, Lancha AH Jr, Costa Rosa LF. Effect of arginine, ornithine and citrulline supplementation upon performance and metabolism of trained rats. Cell Biochem Funct. 2003 Mar; 21 (1): 85-91.

Møller P, Wallin H, Knudsen LE. Oxidative stress associated with exercise, psychological stress and life-style factors. Chem Biol Interact. 1996 Sep 27; 102 (1): 17-36.

Moon JY, Yang EJ, Kim SS, Kang JY, Kim GO, Lee NH, Hyun CG. Sasa quelpaertensis phenylpropanoid derivative suppresses lipopolysaccharide-induced nitric oxide synthase and cyclo-oxygenase-2 expressions in RAW 264.7 cells. Yakugaku Zasshi. 2011; 131 (6): 961-7.

Park YO, Lim HS. Antioxidant activities of bamboo (Sasa borealis) leaf extract according to extraction solvent. J Korean Soc Food Sci Nutr 2009 38: 1640-1648.

Pratusevich IuM, Alekseeva LA. Effect of enteral administration of supplementary nutritional factors (methionine, glutamic acid, vitamin B complex and yeast) on brain blood circulation during chronic mental fatigue in children. Dokl Akad Nauk SSSR. 1971; 198 (5): 1245-8.

Rannem T, Ladefoged K, Hylander E, Christiansen J, Laursen H, Kristensen JH, Linstow M, Beyer N, Liguori R, Dige-Petersen H. The effect of selenium supplementation on skeletal and cardiac muscle in selenium-depleted patients. JPEN J Parenter Enteral Nutr. 1995 Sep-Oct; 19 (5): 351-5.

Ryou SH, Kang MS, Kim KI, Kang YH, Kang JS. Effects of green tea or Sasa quelpaertensis bamboo leaves on plasma and liver lipids, erythrocyte Na efflux, and platelet aggregation in ovariectomized rats. Nutr Res Pract. 2012 Apr; 6 (2): 106-12. Epub 2012 Apr 30.

Savitski IV, Mardashko AA, Popik GS. Lactate dehydrogenase isoenzyme spectrum in the myocardium and skeletal muscles of animals under physical stress. Ukr Biokhim Zh. 1979 Jan-Feb; 51 (1): 45-9.

Shaw S, Jayatilleke E. The role of cellular oxidases and catalytic iron in the pathogenesis of ethanol-induced liver injury. Life Sci. 1992; 50 (26): 2045-52.

Wagenmakers AJ. Amino acid metabolism, muscular fatigue and muscle wasting. Speculations on adaptations at high altitude. Int J Sports Med. 1992 Oct; 13 Suppl 1: S110-3.

An object of the present invention to provide a composition for improving exercise performance and fatigue using jeju jeoldae extract.

Other and further objects of the present invention will be described below.

The present inventors, as confirmed in the Examples and Experimental Examples below, the jeju jeoldae ( sasa) through the DPPH experiment quelpaertensis leaves and common stalk ( Sasa The inhibitory activity of reactive oxygen species on leaf of borealis ) was compared, and the expression level of heme oxygenase-1 (HO-1), an antioxidant enzyme, was also evaluated. Antioxidant activity of the leaves of Jeju barley leaves was more than twice as high as those of normal barley leaves when the concentration was 2mg / mL or 4mg / mL through the free radical scavenging ability of DPPH. In the cells, the amount of protein expression of HO-1 was also found to be significantly higher Jeju jejudae leaves. As mentioned above, reactive oxygen species are generated in the body when physical stress such as continuous exercise occurs, and the antioxidant action to remove them is one of the important mechanisms for overcoming stress. In this regard, it was also confirmed that the mass of adrenal gland increased due to stress was decreased in the group treated with Jeju leaf extract.

In addition, as confirmed in the following Examples and Experimental Examples, Sasa quelpaertensis leaves and Sasa general Borealis ) leaf analysis showed that the amino acid composition of Jeju barley leaves, including valine, leucine, isoleucine, arginine, methionine, and aspartic acid, was 4,762.5 mg / 100 g, which is 1.5 times higher than 3,156.7 mg / 100 g of ordinary barley. The selenium (Se) content was 4.4 times higher than that of general stalks. Among the amino acids, valine, leucine, isoleucine, arginine, methionine, and aspartic acid have muscle strength, endurance, and anti-fatigue activity. Functional and muscle function (Pratusevich and Alekseeva, 1971; Guarnieri et al., 1979; Meneguello et al., 2003; Abel et al., 2005; Chariot and Bignani, 2003; Rannem et al., 1995 ).

As mentioned above, amino acid, selenium, etc. related to the anti-fatigue activity, endurance strengthening function, and muscle strengthening function of Jeju walrus leaves have superior antioxidant activity compared to general walrus leaves, and Jeju walnut leaves compared to general walnut leaves. The fact that it contains a large amount of ingredients can support the fact that Jeju Jelly leaves have better exercise performance and fatigue recovery activity than general Jelly leaves.

The present invention is provided based on the experimental results, (i) in one aspect the present invention can be understood as a composition for improving exercise performance comprising the jeju jeoldae leaf extract as an active ingredient, (ii) to another aspect In the jeju jeoldae leaf extract can be identified as a composition for fatigue recovery comprising as an active ingredient.

In the present specification, "Jeju jeoldae extract" refers to the jeju jeoldae leaves to be extracted water, methanol, ethanol, butanol lower alcohol having 1 to 4 carbon atoms, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloro The crude extract obtained by using methane, N, N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof and the crude extract thereof are listed above. Refers to a fraction obtained by fractionation into one or more of; The extraction method can be applied by any method such as cold-rolling, refluxing, heating, ultrasonic irradiation or the like in consideration of the degree of extraction and the degree of preservation of the active ingredient. In the case of the fractionated extract, the crude extract is suspended in a specific solvent and mixed and fixed with a solvent having a different polarity. The crude extract is adsorbed onto a column filled with silica gel and the like, followed by a hydrophobic solvent, a hydrophilic solvent, or a mixture thereof. It is meant to include a fraction obtained by using a solvent as a mobile phase. Also, the meaning of the extract includes a concentrated liquid extract or a solid extract in which the extraction solvent is removed by a method such as freeze drying, vacuum drying, hot air drying, spray drying and the like. Preferably it refers to an extract obtained by using water, ethanol or a mixed solvent thereof as the extraction solvent, more preferably an extract obtained by using a mixed solvent of water and ethanol as the extraction solvent.

In addition, in the present specification, "active ingredient" means a component that can exhibit the desired activity alone or in combination with a carrier that is not active itself.

The composition for improving exercise performance and the fatigue recovery composition (hereinafter referred to as "composition of the present invention") of the present invention may be used according to the use, formulation, purpose of formulation, etc. as long as the active ingredient can exhibit the intended activity. It may be included in any amount (effective amount), and a conventional effective amount will be determined within the range of 0.001% to 99.990% by weight based on the total weight of the composition. The term "effective amount" as used herein refers to the amount of the active ingredient capable of inducing the intended activity. Such effective amounts can be determined experimentally within the ordinary skill of those skilled in the art.

The composition of this invention can be grasped | ascertained as a food composition in a specific aspect.

When the composition of the present invention is understood as a food composition, the form of food is tea, juice, carbonated beverages, beverages such as ionic beverages, processed oils such as milk, yogurt, gums, rice cakes, sweets, bread, sweets, noodles, etc. Health food preparations such as foods, powders, tablets, capsules, and the like.

The food composition of the present invention may contain sweetening agents, flavoring agents, physiologically active ingredients, minerals and the like in addition to the active ingredients thereof.

Sweetening agents may be used in an amount that sweetens the food in a suitable manner, and may be natural or synthetic. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavors may be used to enhance taste or flavor, both natural and synthetic. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. The natural flavor may be obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or may be obtained from green tea leaves, round leaves, jujube leaves, cinnamon, chrysanthemum leaves, jasmine and the like. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. Synthetic flavors may be used depending on the case, and synthetic flavors such as esters, alcohols, aldehydes, terpenes and the like may be used.

Examples of the physiologically active substance include catechins such as catechin, epicatechin, gallocatechin and epigallocatechin, and vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine and riboflavin.

As the mineral, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium and zinc can be used.

In addition, the food composition of the present invention may contain preservatives, emulsifiers, acidifiers, thickeners and the like as needed in addition to the above sweeteners.

Such preservatives, emulsifiers and the like are preferably added in a very small amount as long as they can attain an application to which they are added. The term " trace amount " means, when expressed numerically, in the range of 0.0005% by weight to about 0.5% by weight based on the total weight of the food composition.

Examples of the preservative which can be used include calcium sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate and EDTA (ethylenediaminetetraacetic acid).

Examples of the emulsifier which can be used include acacia gum, carboxymethyl cellulose, xanthan gum, pectin and the like.

Examples of the acidulant that can be used include acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, and phosphoric acid. Such an acidulant may be added so that the food composition has a proper acidity for the purpose of inhibiting the growth of microorganisms other than the purpose of enhancing the taste.

Agents that may be used include suspending agents, sedimentation agents, gel formers, bulking agents and the like.

The composition of the present invention may be regarded as a pharmaceutical composition in another specific embodiment.

The pharmaceutical composition of the present invention may be formulated into a wide variety of dosage forms, including pharmaceutically acceptable carriers, excipients and the like, in addition to the active ingredient, and may be formulated into a wide variety of preparations such as creams, lotions, ointments (semi-solid external medicine) (TTS) (e.g., patches, bandages, etc.), and the like.

The term "pharmaceutically acceptable" as used herein means that the application (prescribing) subject does not have the above-mentioned toxicity that is adaptable without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (such as corn starch, potato starch, etc.), cellulose, derivatives thereof (such as sodium carboxymethyl cellulose, ethylcellulose, etc.), malt, gelatin, talc, Solid lubricants (such as stearic acid, magnesium stearate, etc.), calcium sulfate, vegetable oils (such as peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (such as propylene glycol, glycerin, etc.), alginic acid, and the like. One or more may be selected and used according to the formulation of the pharmaceutical composition of the present invention. For suitable pharmaceutically acceptable carriers and formulations, see Remington's. Pharmaceutical Sciences (19th ed., 1995). The pharmaceutical compositions of the present invention may further comprise emulsifiers (such as TWEENS), wetting agents (such as sodium lauryl sulfate), colorants, flavors, stabilizers, preservatives, water, saline, phosphate buffer solutions, and the like.

The excipient may be selected according to the formulation of the pharmaceutical composition of the present invention. For example, suspending agents such as sodium carboxymethyl cellulose, methyl cellulose, hydropropyl methyl cellulose, sodium alginate, polyvinyl pyrrolidone, .

The daily dose of the pharmaceutical composition of the present invention is usually 0.001 to 150 mg / kg body weight, and may be administered once or several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as route of administration, age, sex, weight, and patient's severity of the patient, the dose is limited in any aspect to the scope of the present invention Should not be understood to be.

As described above, according to the present invention can provide a composition for improving exercise performance and fatigue using jeju jeoldae extract.

The composition of the present invention may be commercialized as a food or drug.

1 is a result showing that the antioxidant extract of Jeju jjoredae leaf extract is superior to the general jjoredae leaf extract through the DPPH test method.
2 is a result showing that the Jeju leaf extract extract LPS-induced NO scavenging activity in RAW263.7 cells is superior to the general leaf extract.
3 is a result showing that the Jeju Joridae leaf extract is superior to the induction activity of the antioxidant enzyme HO-1 in RAW263.7 cells than the common sagogi leaf extract

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.

< Example > Preparation of Jeju Sorrel Leaf Extract

Sasa 6 kg of quelpaertensis leaves were washed with tap water, lyophilized and ground. The pulverized Jeju chopsticks were immersed in water to extract hot water at 90 ° C. for 4 hours, and then concentrated under reduced pressure to remove the extraction solvent to obtain the extract of Jeju chopsticks in powder form.

< Comparative Example > Preparation of Common Squash Leaf Extract

Common Sacks ( Sasa 6 kg of borealis ) leaves were washed with tap water, lyophilized and ground. The pulverized Jeju chopsticks were immersed in water to extract hot water at 90 ° C. for 4 hours, and then concentrated under reduced pressure to remove the extraction solvent to obtain the extract of Jeju chopsticks in powder form.

< Experimental Example > Activity Confirmation Test and General Component Analysis of Jeju Leaf Extract

Experimental Example 1 Antioxidant Activity Test of Jeju Leaf

Experimental Example 1-1 Experimental Method

DPPH  Free radical scavenging activity

The electron donating ability of the sample was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH; Sigma, USA) according to the Blosis method (Blois, 1958). First, 80% ethanol extract diluted in methanol was dispensed into 96 well plates at 100 μl in various concentrations, and 0.4 mM DPPH solution was added in the same amount. Thereafter, the 96 well plate was reacted for 20 minutes in a light shielding state, and the absorbance was measured at 517 nm. Absorbance values were normalized based on the absorbance of the sample, ascorbic acid (Sigma, USA) was used as a control, and scavenging ability was calculated as follows. The concentration of the sample that appears when the absorbance of DPPH was reduced by 50% was expressed as IC ₅₀ , and the experiment was repeated three times for each sample and expressed as an average value.

DPPH radical scavenging activity (%) = 100 × (A _control -A _sample ) / A _control

A _control = Absorbance of the reaction solution containing only methanol

A _sample = Absorbance of the reaction solution to which the sample is added

Cell culture

Mouse RAW 264.7 macrophage lines were purchased from Korean Cell Line Bank. Cells were treated at 37 ° C. using Dulbeccos's Modifid Eagle Medium (DMEM; Gibco, USA) medium containing 10% fetal bovine serum (FBS; Gibco, USA) and 1% penicillin / streptomycin (P / S; Gibco, USA). 5% CO ₂ Cultured in a thermostat.

NO  Production measurement

NO produced from RAW 264.7 cells was measured by adding Griess reagent to the cell culture. 100 μl of the cell culture solution and 100 μl of Griess reagent [1% (w / v) sulfanilamide, 0.1% N-1-napthylethylene diamine in 2.5% (v / v) phosphoric acid] were mixed for 10 minutes in a 96 well plate. Absorbance was measured at 540 nm. Sodium nitrite was used to calculate the standard curve, and the generated NO value was calculated.

HO -1 expression level

RAW 264.7 cells were placed in a 12 well cell culture plate at 5 × 10 ⁵ cells / well, incubated for 24 hours, sample incubated for one hour, and cultured for 18-24 hours by adding LPS to 100 ng / ml. The cells were then washed twice with cold PBS and lysis buffer [1 × RIPA (Upstate Cell Signaling Solution, Lake Placid, NY, USA), 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM NaVO ₄ , 1 mM NaF, 1 μg / ml aprotinin, 1 μg / ml pepstatin, and 1 μg / ml leupeptin] were lysed for 1 hour, followed by centrifugation (15,000 rpm, 15 min) to separate only the protein supernatant. Protein concentration was quantified using Bio-Rad protein assay reagent with bovine serum albumin (BSA) as a standard. Quantitative proteins were electrophoresed on 8-12% polyacylamid gel and transferred to polyvinyllidene difluoride (PVDF) membrane (Milipore, USA) for 250 mA for 180 minutes. The protein-transferred membrane was placed in 0.05% Tween 20 / Tris-buffered saline (0.05% T / TBS) containing 5% skim milk powder, blocked at room temperature for 1 hour, and reacted with the primary antibody. The primary antibody reaction was reacted overnight at 4 ° C. using HO-1 antibody (1: 5,000, Calbiochem, USA). After the first antibody reaction, the membrane was washed three times with 0.05% T / TBS solution, and then peroxidase-conjugated secondary antibody (Jackson ImmunoResearch, USA) was diluted 1: 5,000 or 1: 10,000 and reacted at room temperature for 1 hour. Wash three times with 0.05% T / TBS solution. Protein was confirmed by X-ray film using the enhanced chemiluminescence (ECL) method.

Experimental Example 1-2 Experimental Results

Experimental results of Jeju stalks and stalks for antioxidant activity are shown below in [FIG. 1], [FIG. 2], and [FIG. 3].

As shown in the results of Figs. 1, 2 and 3, Jeju marinade had a higher ability to remove reactive oxygen species compared to general girdle, and had an antioxidant function in the group treated with high concentration of Jeju marinade. Heme oxygenase-1 (HO-1) -induced activity was also high.

Experimental Example 2 Effect of Jeju Joridae Extract on Adrenal Weight Reduction in Stress-induced Rats

Experimental Example 2-1 Experimental Method

Experimental animal model production

Four-week-old SD rats were acclimated for one week with solid feed and water. Each group was randomly selected to 10 groups (about 166g / rat) into 3 groups, and two dogs were separated and raised in a cage. Group 3 was composed of a normal group, a control group, and a sample administration group (JSQ).

Restraint stress was performed for 7 days at 5h / day using the fabricated framework. Jeju Joridae extract 150mg / kg / day was administered orally from 1 week before the stress stimulation, and 30 minutes before the stress stimulation from the time of stress stimulation.

In order to confirm the increase and decrease of body weight and adrenal weight of the experimental animals due to stress after the end of the experiment, the adrenal glands of the animals were removed, washed with saline, and then drained, and the weight of organs was measured.

<Experimental Example 2-2> results

The experimental animals lost weight compared to the normal group due to stress, but there was no difference in weight between the control group and the Jeju-derived group. However, the weight of adrenal gland increased in the stressed control group compared to the normal group, but significantly decreased compared to the control group when treated with Jeju jejundae [Table 1].

Experimental group Weight (g) Adrenal gland (mg / body weight) Normal 249.75 ± 3.63 0.085 ± 0.006 Control 222.12 ± 5.76 0.108 ± 0.003 JSQ (Jeju Joridae Treatment Group) 224.47 ± 4.77 0.094 ± 0.003

Experimental Example 2-3 Amino Acid Content and General Component Analysis

Amino acids were analyzed by ninhydrine HPLC analysis, general components (calories, moisture, fat protein, ash, carbohydrates and dietary fiber) were analyzed according to the Food Code, minerals were analyzed by ICP-AES measurement.

The results are shown in the following [Table 2] and [Table 3].

As described above, amino acids such as valine, leucine, isoleucine, arginine, methionine, and aspartic acid have muscle strength and endurance improvement, anti-fatigue activity, and selenium has effects such as immune function and muscle function improvement. As a result of analyzing the amino acid composition in the two stalks, the constituent amino acid of Jeju stalk leaves was 4,762.5mg / 100g, which was 1.5 times higher than the general sack, and the selenium (Se) content was 4.4 times higher than that of the general sack.

Claims (8)

A composition for improving exercise performance, comprising Jeju leaf extract as an effective ��.
The method of claim 1,
Wherein the extract jeju jeoldae leaves using the jeju jeoldae leaves using water, ethanol or a mixed solvent thereof as an extraction solvent, the composition for exercise performance improvement.
3. The method according to claim 1 or 2,
The composition is a composition for improving athletic performance, characterized in that the food composition.
3. The method according to claim 1 or 2,
The composition is a composition for improving exercise performance, characterized in that the pharmaceutical composition.
Fatigue recovery composition comprising jeju jeoldae leaf extract as an active ingredient.
6. The method of claim 5,
The extract of Jeju jeoridae is a composition for fatigue ash, characterized in that obtained by using the jeju jeori leaves water, ethanol or a mixed solvent thereof as an extraction solvent.
The method according to claim 5 or 6,
The composition for fatigue recovery, characterized in that the food composition.
The method according to claim 5 or 6,
The composition for fatigue recovery, characterized in that the pharmaceutical composition.

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