KR101416572B1 - Composition for antiaging comprising extract or fractions of Mastixia arborea C.B.Clarke as an active ingredient - Google Patents

Abstract

The present invention relates to an antioxidant composition containing an extract of Mastixia arbora CBClarke or a fraction thereof as an active ingredient. More specifically, the mastisia alboria extract of the present invention or a fraction thereof has a DPPH Free radical scavenging activity and ABTS free radical scavenging activity and has an excellent antioxidative effect, it can be usefully used as an active ingredient of a cosmetic composition for preventing aging, a pharmaceutical composition and a health food.

Description

[0001] The present invention relates to an antioxidant composition containing an extract of mastisia alboria or a fraction thereof as an active ingredient,

More particularly, the present invention relates to a cosmetic composition for preventing deterioration, a pharmaceutical composition and a health food containing an extract of Mastixia arbora CBClarke or a fraction thereof as an active ingredient.

Free radicals that cause aging and disease in humans are known to form in biological reactions during normal metabolic processes in the body, causing harmful toxins to cells and tissues and causing various diseases (Folin O & Denis W, 1912, J. Biol. Chem. 12: 239-249). Physiological actions to inhibit such harmful free radicals include electron donating ability to inhibit oxidation by donating electrons to oxidative free radicals and superoxide anion (SOD), which has a similar reactivity with superoxide dismutase (SOD) It has the role of converting radical (superoxide anion radical) into normal oxygen. Several physiologically active ingredients with strong antioxidant effects contribute to prevention of ulcers and seizures caused by oxidative stress, regulation of gastric secretion and prevention of diarrhea, prevention of diabetes, prevention and reduction of cancer, heart disease and degenerative diseases (Azuma K et al ., 1999, J. Agric. Food Chem. 47: 3963-3966, Ham SS et al ., 1997, J. Food Sci. Nutr. 2: 155-161). Recently, as interest in health has increased, a resource containing a large amount of a functional substance having various effects such as anticancer, antiallergic, anti-obesity, antioxidant and antibacterial activity has been selected from various plant resources, there is research to develop a material is going around the world (Shin DB, et al, 2006 , Food Sci Biotechnol 15:.... 357-362, Park et al, 2004, Korean J. Nutr 37:. 364- 372, Kim et al ., 2006, Kor J. Food Sci. Technol. 38: 799-804, Ali KA et al ., 2005, Food Chem. 89: 27-36).

Superoxide dismutase, an antioxidant, breaks down the active oxygen generated by the respiration process of cells, ie, superoxide radicals into hydrogen peroxide and oxygen. Hydrogen peroxide reacts with metal ions such as iron or copper to generate more active oxygen, called hydroxy radical. Catalase and glutathione peroxidase, antioxidants that remove hydrogen peroxide before it reacts with metal ions, decompose hydrogen peroxide into water and carbon dioxide. The antioxidant that removes hydrogen peroxide can not completely remove 5% of hydrogen peroxide, which reacts with metal ions in the body to turn into hydroxy radicals, aging our bodies and causing them to die. Frequent exposure to radiation or ultraviolet light changes the hydrogen peroxide into hydroxy radicals to promote senescence. An antioxidant is a method of delaying this. Superoxide radicals (O ^2- ) itself have relatively low reactivity, but they are easily converted to H ₂ O ₂ , which in turn generates hydroxyl radicals that are highly reactive or react with nitric oxide (NO) (Peroxynitrite) (ONOO), which causes oxidation of SH-group, nitration of protein tyrosine, lipid peroxidation, and DNA damage.

Antioxidants are widely distributed in copper and plants. Many antioxidants such as phenolic compounds, flavonoids, tocopherols, vitamin C and selenium in fruits and vegetables can delay or prevent the oxidation of fats and cause cancer and cardiovascular diseases Prevention, and delaying the disease. Tocopherol and vitamin C, which are natural antioxidants in the vegetable field, are widely used in foods, medicines and cosmetics. The rancidity of foods containing oil or fat is mainly caused by binding with oxygen in the air. To prevent this, many synthetic or natural antioxidants have been developed. However, synthetic antioxidants are used in practice because of their effectiveness and economy. Examples of synthetic antioxidants include Butylated Hydroxy Toluene (BHT), Butylated Hydroxy Anisole (BHA), and Propyl Gallate. Synthetic antioxidants are widely used in commercial foods because of their excellent antioxidant ability and low cost, but their use is regulated due to concerns about safety, such as human side effects. Natural antioxidants such as tocopherol are safe for the human body, but have the disadvantage that they have the ability to inhibit oxidative chain reaction and are expensive. Therefore, in recent years, researches for the development of natural antioxidants which are more safe and have excellent antioxidative effects have been actively conducted.

On the other hand, Mastixia arborea CBClarke belongs to the family Cornaceae and is native to India and Sri Lanka. Although the plant bark has been known to have a component called loganin (Wieffering, JH, 1966, Phytochemistry), studies on the activity of extracts and components of mastisia alboria are not yet known.

Therefore, the inventors of the present invention found that, while screening plants having excellent antioxidative effects, the DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical scavenging activity and the ABTS (2,2'-azinobis - (3-ethylbenzothiazoline-6-sulphonic acid)) free radical scavenging activity, and its fractions also showed excellent antioxidative activity, confirming that the extract of mastisia albaoria and its fractions are effective for the anti- The present invention has been accomplished on the basis of these findings.

An object of the present invention is to provide an anti-aging cosmetic composition, a pharmaceutical composition and a health food containing an extract of Mastixia arbora CBClarke or a fraction thereof as an active ingredient.

In order to achieve the above object, the present invention provides an anti-aging cosmetic composition containing an extract of Mastixia arbora CBClarke or a fraction thereof as an active ingredient.

The present invention also provides a pharmaceutical composition for preventing premature aging comprising an extract of mastisia alboria or a fraction thereof as an active ingredient.

In addition, the present invention provides an anti-aging health food comprising an extract of mastisia alboria or a fraction thereof as an active ingredient.

Mastixia arborea CBClarke extract of the present invention showed inhibitory effect on DPPH free radical generation and ABTS free radical generation in a concentration dependent manner and its fractions also showed excellent antioxidative effect, Cosmetic compositions, pharmaceutical compositions, and health foods.

FIG. 1 is a drawing showing a process for preparing Mastixia arborea CBClarke extract and fractions.
FIG. 2 shows TLC (Thin Layer Chromatography) of extracts and fractions of Mastixia arborea CBClarke;
T: mastisia alboria extract;
H: mastisia alboria n-hexane fraction;
C: mastisia alboria chloroform fraction;
E: fraction of mastisia alboria ethyl acetate;
B: mastisia alboria butanol fraction; And
W: Water fraction of mastisia alboria.
FIG. 3 is a diagram showing CAD (Charge Aerosol Detector) of Mastixia arborea CBClarke extract and fractions.
4 is a graph showing the DPPH free radical scavenging activity of Mastixia arborea CBClarke extract.
5 is a graph showing the ABTS free radical scavenging activity of Mastixia arborea CBClarke extract.

Hereinafter, the present invention will be described in detail.

The present invention provides an anti-aging cosmetic composition containing an extract of Mastixia arbora CBClarke or a fraction thereof as an active ingredient.

The aging is preferably, but not exclusively, skin aging.

The present invention also provides an antioxidant cosmetic composition comprising an extract of Mastixia arbora CBClarke or a fraction thereof as an active ingredient.

The mastisia alboria extract is preferably but not limited to be produced by a method comprising the steps of:

1) extracting Mastixia arborea CBClarke with an extraction solvent;

2) filtering the extract of step 1); And

3) A step of concentrating the filtered extract of step 2) under reduced pressure and then drying to prepare an extract of mastisia alboria.

In the above method, the Mastixia arborea CBClarke of step 1) may be used without limitation such as grown or commercially available. The mastisia alboria may be leaves, stalks or roots but is not limited thereto.

In the above method, it is preferable to use water, an alcohol or a mixture thereof in the extraction solvent of the step 1). As the alcohol, a C ₁ to C ₂ lower alcohol is preferably used. As the lower alcohol, ethanol or methanol is preferably used, and methanol is most preferably used, but not limited thereto. As the extraction method, it is preferable to use shaking extraction, Soxhlet extraction or reflux extraction, but it is not limited thereto. The extraction solvent is preferably added in an amount of 1 to 10 times the amount of the dried masticia alboria, and more preferably 2 to 3 times of the extraction solvent. However, the extraction is not limited thereto. The extraction temperature is preferably 20 to 100 ° C, and preferably 20 to 40 ° C, but is not limited thereto. In addition, the extraction time is preferably 10 to 48 hours, more preferably 15 to 30 hours, but is not limited thereto. In addition, the extraction number is preferably 1 to 5 times, more preferably 3 to 4 times, but is not limited thereto.

In the above method, it is preferable to use a vacuum decompression concentrator or a vacuum rotary evaporator for the decompression concentration in step 3), but it is not limited thereto. The drying is preferably performed under reduced pressure, vacuum drying, boiling, spray drying or freeze drying, but not always limited thereto.

The fraction of the mastisia alboria extract of the present invention is preferably produced by a method of extracting the mastisia alboria extract with an organic solvent, but is not limited thereto.

The organic solvent is preferably n-hexane, chloroform, ethyl acetate, or butanol, but is not limited thereto. The fractions were n-hexane fraction, chloroform fraction, ethyl acetate fraction and butanol fraction obtained by adding n-hexane, chloroform, ethyl acetate and butanol stepwise and then obtaining a soluble fraction in each solvent addition step, The water fraction obtained by removing the fraction and concentrating the remaining water layer can be used, but preferably the butanol or ethyl acetate fraction.

In a specific example of the present invention, in order to confirm the antioxidative effect of the extract of mastisia alboria, the DPPH free radical scavenging activity was measured by using 1,1-diphenyl-2-picryl-hydrazyl picryl-hydrazyl, DPPH, Sigma). As a result, it was confirmed that the mastisia alboria extract showed DPPH free radical scavenging activity in a concentration-dependent manner (see FIG. 4).

Further, in another specific example of the present invention, the DPPH and ABTS free radical scavenging activities of the mastisia alboria solvent fraction were confirmed, and the mastisia alboria solvent fraction showed excellent DPPH and ABTS free radical scavenging effects (See Table 1).

Further, in another specific example of the present invention, in order to confirm the antioxidative effect of the extract of mastisia alboria, the ABTS free radical scavenging activity according to various concentrations of the mastisia alboria extract was measured, It was confirmed that the boria extract exhibited an ABTS free radical scavenging activity in a concentration-dependent manner (see FIG. 5).

Therefore, the mastisia alboria extract of the present invention or its fractions exhibits DPPH free radical scavenging activity and ABTS free radical scavenging activity in a concentration-dependent manner and has an excellent antioxidative effect. Therefore, the cosmetic composition for antioxidation or the cosmetic composition for antioxidation Can be used effectively.

The cosmetic composition may be in the form of a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic (liposome) A cream, a skin, a lotion, a powder, an ointment, a spray, or a cone stick. It can also be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

The cosmetic composition may further contain, in addition to the mastisia albaoria extract of the present invention or a fraction thereof, a lipid, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softener, an antioxidant, a suspending agent, a stabilizer, ), Perfumes, surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipids Or any other ingredient conventionally used in cosmetics, to name but a few.

In the cosmetic composition containing the mastisia alboria extract of the present invention or a fraction thereof, the extract of the present invention is added to the cosmetic composition usually contained in an amount of 0.1 to 50% by weight, preferably 1 to 10% by weight .

When the mastisia albaoria extract of the present invention or a fraction thereof is used as an external preparation for skin, it may further contain a fatty substance, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softener, an antioxidant, a suspending agent, a stabilizer, a surfactant, a water, an ionic or nonionic emulsifier, a filler, a sequestering and chelating agent, a preservative, a vitamin, a barrier, a wetting agent, an essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent, Such as lipid vesicles or any other ingredient conventionally used in external preparations for skin. In addition, the components can be introduced in amounts commonly used in the dermatology field.

The present invention also provides a pharmaceutical composition for preventing premature aging comprising an extract of mastisia alboria or a fraction thereof as an active ingredient.

The aging is preferably, but not exclusively, skin aging.

In addition, the present invention provides a pharmaceutical composition for antioxidant containing an extract of mastisia albaoria or a fraction thereof as an active ingredient.

The mastisia alboria extract of the present invention or its fractions exhibits DPPH free radical scavenging activity and ABTS free radical scavenging activity in a concentration dependent manner and has an excellent antioxidative effect. Therefore, it is an effective ingredient of the anti-aging pharmaceutical composition or the antioxidant pharmaceutical composition Can be used effectively.

The composition containing the mastisia alboria extract of the present invention or a fraction thereof may further contain one or more active ingredients which exhibit the same or similar functions in addition to the above components.

The composition of the present invention may further comprise a pharmaceutically acceptable additive, wherein pharmaceutically acceptable additives include starch, gelatinized starch, microcrystalline cellulose, lactose, povidone, colloidal silicon dioxide, calcium hydrogen phosphate, lactose Starch glycolate, sodium starch glycolate, carnauba wax, synthetic aluminum silicate, stearic acid, magnesium stearate, aluminum stearate, calcium stearate, calcium stearate, , White sugar, dextrose, sorbitol and talc. The pharmaceutically acceptable additives according to the present invention are preferably included in the composition in an amount of 0.1 to 90 parts by weight, but are not limited thereto.

That is, the composition of the present invention can be administered in various formulations of oral and parenteral administration at the time of actual clinical administration. In the case of formulation, a diluent such as a filler, an extender, a binder, a wetting agent, a disintegrant, . ≪ / RTI > Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient, such as starch, calcium carbonate, Sucrose, lactose, gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate talc may also be used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions and syrups, and various excipients such as wetting agents, sweetening agents, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used . Formulations for parenteral administration may include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like can be used as the non-aqueous solvent and suspension agent. Examples of suppository bases include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, and the like.

The composition of the present invention may be administered orally or parenterally in accordance with the intended method, and may be administered orally, parenterally or intraperitoneally, rectally, subcutaneously, intravenously, intramuscularly, . The dosage varies depending on the patient's body weight, age, sex, health condition, diet, administration time, administration method, excretion rate, and disease severity.

The dosage of the composition of the present invention varies depending on the patient's body weight, age, sex, health condition, diet, administration time, administration method, excretion rate and severity of disease, The amount of the extract is 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg, and can be administered 1 to 6 times a day.

The composition of the present invention may be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers.

In addition, the present invention provides an anti-aging health food containing an extract of mastisia albaoria or a fraction thereof as an active ingredient.

The aging is preferably skin aging, but not always limited thereto.

The present invention also provides an antioxidant health food containing an extract of mastisia albaoria or a fraction thereof as an active ingredient.

The mastisia alboria extract of the present invention or its fractions exhibits DPPH free radical scavenging activity and ABTS free radical scavenging activity in a concentration dependent manner and has an excellent antioxidative effect and thus is useful as an active ingredient of an anti-aging health food or antioxidant health food Can be used.

The health food of the present invention may be used as it is, or may be used in combination with other food or food ingredients, and may be appropriately used according to a conventional method.

There is no particular limitation on the kind of the health food. Examples of foods to which the above-mentioned mastisia alboria extract or its fractions can be added include dairy products including meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, other noodles, gums, ice cream, Soups, drinks, tea, drinks, alcoholic beverages, and vitamin complexes, all of which include health foods in a conventional sense.

The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The ratio of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.

In addition to the above, the health food of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acids and salts thereof, alginic acid and its salts, organic acids, protective colloid thickeners, pH adjusters, stabilizers, preservatives, glycerin, , A carbonating agent used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

The present invention also provides a method for inhibiting senescence comprising administering to a subject a pharmaceutical composition comprising a pharmaceutically effective amount of a mastisia albaoria extract or a fraction thereof as an active ingredient.

The aging is preferably, but not exclusively, skin aging.

The pharmaceutically effective amount is 0.00001 to 10 mg / kg, preferably 0.0001 to 1 mg / kg, but is not limited thereto. The dose may vary depending on the weight, age, sex, health condition, diet, administration period, method of administration, rate of elimination, severity of disease, and the like of a particular patient.

The subject is a vertebrate animal, preferably a mammal, more preferably an experimental animal such as a mouse, a rabbit, a guinea pig, a hamster, a dog or a cat, and most preferably an ape-like animal such as a chimpanzee or a gorilla.

The above administration method is preferably oral or parenteral, and skin application is most preferred.

Hereinafter, the present invention will be described in detail with reference to Examples, Experimental Examples and Production Examples.

However, the following Examples, Experimental Examples and Preparation Examples are merely illustrative of the present invention, and the contents of the present invention are not limited by Examples, Experimental Examples and Production Examples.

≪ Example 1 > Preparation of mastitia alboria extract

The mastisia alboria extract of the present invention was purchased from the overseas plant extract bank of the International Biomaterials Hub Center of the Korea Research Institute of Bioscience and Biotechnology, and a sample of evidence was obtained from the Herbarium of the Korea Research Institute of Bioscience and Biotechnology (KRIB) (KRIB 0019270). The extracts were prepared by drying the mastisia alboria (leaf, young eggplant), adding 1 L of methanol to 67 g of the pulverized powder sample, using an ultrasonic extractor (SDN-900H, SD-ULTRASONIC CO., LTD) And extracted repeatedly 30 times at room temperature (40 KHz, 15 minutes extraction - 120 minutes). Thereafter, the mixture was filtered and concentrated under reduced pressure, to obtain 4.13 g of a mastisia alboria methanol extract (hereinafter referred to as mastisia alboria extract).

Example 2 Preparation of Solvent Fraction of Martian Alvoria

The same amount of n-hexane was added to and mixed with 430 mg of the masticia albooria extract obtained in Example 1, followed by distillation. The n-hexane soluble fraction and the water soluble fraction were separated, The mixture was filtered, concentrated under reduced pressure, and 93.4 mg of n-hexane fraction was obtained. Then, the n-hexane fraction was removed and chloroform was equally added to the remaining water layer to obtain 69.6 mg of a chloroform fraction. To the remaining water layer, ethyl acetate was added in the same amount to obtain 84.4 mg of an ethyl acetate fraction. An equal amount of butanol was added to the remaining water layer, and 72.6 mg of a butanol fraction was obtained in the same manner. The remaining water layer was concentrated to obtain 78.1 mg of a water fraction (FIG. 1).

≪ Example 3 > TLC investigation of mastitia alboria extract and fractions

The mastisia alboria extract and fractions obtained in Example 1 and Example 2 were dissolved in methanol at a concentration of 10 mg / ml and subjected to silica gel thin layer chromatography (TLC Silica gel 60 F ₂₅₄ , Merck) . As a developing solvent, a mixed solvent of n-hexane: acetone at a ratio of 2: 1 was used. Also, silica gel reverse phase chromatography (TLC Silica gel 60 RP-18 F ₂₅₄ S, Merck) was performed and the developing solvent was 90% methanol (FIG. 2).

Example 4 UPLC analysis of extracts and fractions of mastisia alboria

The mastisia alboria extract and fractions obtained in Example 1 and Example 2 were dissolved in methanol at a concentration of 10 mg / ml and filtered through a membrane filter of 0.2 μm. The HPLC was performed using ACQUITY UPLC ^™ BEHC ₁₈ (10 mm × 2.1 mm, id, 1.7 μm, waters, USA) at 35 ° C., The mobile phase A was initially treated with water and formic acid (100: 0.1, v / v) and the mobile phase B with acetonitrile and formic acid (100: 0.1, v / v) After increasing the mobile phase B to 100%, the mobile phase B was maintained at 100% for 9 minutes, the mobile phase B was decreased to 5% for 9.5 minutes, and the 5% And used for analysis. The flow rate of the mobile phase was 0.4 ml / min and the sample injection volume was 3 μl.

The CAD (Charge Aerosol Detector) is a detector that measures the charge by attaching a charge to materials separated from the UPLC. The relative content and the degree of separation of the material can be confirmed by a chromatographic method (FIG. 3).

EXPERIMENTAL EXAMPLE 1 Determination of DPPH Free Radical Scavenging Activity of the Extract of M. alboria

The free radical scavenging activity of the mastisia alboria extract of Example 1 was measured using 1,1-diphenyl-2-picryl-hydrazyl, DPPH, Sigma ) Method.

Specifically, for measurement of free radical scavenging activity, 90 μl of a 0.15 mM DPPH solution well dissolved in ethanol was placed in a 96-well plate, and the extract was divided into 2.5, 5, 10, 20, 40 and 80 μg / , Left at room temperature for 20 minutes, and then measured for absorbance at 517 nm with an ELISA reader. The free radical scavenging activity (%) was calculated by the following equation (1). At this time, the group treated with DMSO was used as a negative control, the group treated with BHA, a synthetic antioxidant, and alpha - tocopherol, a natural antioxidant, were used as positive control.

A: Absorbance of the negative control not treated with the mastisia alboria extract of the present invention; And

B: Absorbance of the experimental group treated with the extract of mastisia alboria of the present invention.

As a result, it was confirmed that DPPH free radical scavenging activity was dependent on the treatment concentration of mastisia alboria extract, and it was confirmed that DPPH free radical scavenging activity was superior to BHA and alpha-tocopherol positive control groups 4).

<Experimental Example 2> Determination of ABTS free radical scavenging activity of mastisia alboria extract

The ABTS free radical scavenging activity was confirmed using the mastisia alboria extract of Example 1 above.

Specifically, ABTS free radical scavenging activity was obtained by mixing ABTS and potassium persulfate to a final concentration of 7 mM and 2.45 mM, respectively, and then left in a dark place for 12 hours to form an ABTS radical. This was diluted with distilled water to an absorbance of 0.7 at 734 nm and dispensed into a 96-well plate. The sample was dispensed at a concentration of 2.5, 5, 10, 20, 40 or 80 占 퐂 / And reacted for 7 minutes. Absorbance was measured using a microplate meter at 734 nm. At this time, the group treated with DMSO was used as a negative control, and the group treated with synthetic antioxidant BHA and tokophenol was used as a positive control.

As a result, it was confirmed that ABTS free radical scavenging activity was dependent on the treatment concentration of the extract of mastisia alboria, and ABTS radical scavenging activity was confirmed to be superior to that of BHA and alpha-tocopherol (positive control group 5).

<Experimental Example 3> DPPH and ABTS free radical scavenging ability of the fraction of mastisia alboria solvent

DPPH and ABTS free radical scavenging activity was measured for n-hexane, chloroform, ethyl acetate, butanol and water fractions by the solvent in Example 1 above in the same manner as Experimental Example 1 and Experimental Example 2 . Each fraction was dispensed at a concentration of 40 μg / ml, reacted at room temperature, and the absorbance was measured with a microplate absorber. At this time, the group treated with DMSO was used as a negative control, the group treated with a natural antioxidant a-tocopherol and ascorbic acid, or the synthetic antioxidant BHA was used as a positive control.

As a result, as shown in Table 1, the mastisia alboria solvent fraction showed excellent DPPH and ABTS free radical scavenging effects, and in particular, mastisia alboria ethyl acetate and butanol fraction showed significant DPPH free radical scavenging effect And ABTS free radicals showed excellent scavenging effect over all fractions. In addition, when compared with the group treated with a-tocopherol and ascorbic acid, which are positive control groups derived from natural products, it was confirmed that they exhibited remarkably excellent activity (Table 1).

The sample (40 mu g / ml) DPPH radical scavenging ability (%) ABTS radical scavenging ability (%) Mastisia alboria extract 80.53 ± 0.66 99.93 ± 0.05 Mastisia alboria hexane fraction 14.53 ± 0.70 31.61 ± 0.68 Mastisia alboria chloroform fraction 10.40 ± 0.64 30.81 ± 0.34 Mastisia alboria ethyl acetate fraction 94.19 ± 0.11 99.96 ± 0.09 Mastisia alboria fraction 93.33 ± 0.36 99.73 ± 0.13 Mastisia alboria water fraction 62.58 ± 1.27 98.10 ± 1.28  a-tocopherol 55.89 ± 0.69 83.85 ± 5.17 Ascorbic acid 50.26 ± 0.74 33.49 ± 0.69 BHA (10 [mu] g / ml) 44.94 ± 0.62 99.73 ± 0.07

PREPARATION EXAMPLE 1 Preparation of anti-aging cosmetic ingredients containing mastisia alboria extract or fractions thereof as active ingredients

<1-1> Manufacture of flexible lotion

The softened lotion containing the mastisia alboria extract of Example 1 or a fraction thereof as an active ingredient was prepared as shown in Table 2 below.

Raw material Content (parts by weight) The mastisia alboria extract of the present invention or its fraction 10.00 1,3-butylene glycol 1.00 Disodium iodide 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Glycereth-26 1.00 Arbutin 2.00 Hydrogenated castor oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

<1-2> Preparation of nutritional cream

The nutritional cream containing the mastisia alboria extract of Example 2 or a fraction thereof as an active ingredient was prepared in accordance with the composition shown in Table 3 below.

Raw material Content (parts by weight) The mastisia alboria extract of the present invention or its fraction 10.0 1,3-butylene glycol 7.0 glycerin 1.0 D-Panthenol 0.1 Plant extract 3.2 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlyric / capric triglyceride 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Suitable amount Triethanolamine Suitable amount Preservative Suitable amount Flavoring agent Suitable amount Purified water Balance

PREPARATION EXAMPLE 2 Preparation of pharmaceutical preparations containing mastisia alboria extract as an active ingredient

&Lt; 2-1 >

Mastisia alboria extract or its fractions 2 g

Lactose 1 g

The above components were mixed and packed in airtight bags to prepare powders.

<2-2> Preparation of tablets

100 mg of mastisia alboria extract or its fraction

Corn starch 100 mg

100 mg of milk

Magnesium stearate 2 mg

After mixing the above components, tablets were prepared by tableting according to a conventional method for producing tablets.

&Lt; 2-3 > Preparation of capsules

100 mg of mastisia alboria extract or its fraction

Corn starch 100 mg

100 mg of milk

2 mg of magnesium stearate

After mixing the above components, the capsules were filled in gelatin capsules according to the conventional preparation method of capsules.

&Lt; 2-4 >

Mastisia alboria extract or fraction thereof 1 g

Lactose 1.5 g

Glycerin 1 g

0.5 g of xylitol

After mixing the above components, they were prepared so as to be 4 g per one ring according to a conventional method.

<2-5> Preparation of granules

150 mg of mastisia alboria extract or its fraction

Soybean extract 50 mg

200 mg of glucose

600 mg of starch

After mixing the above components, 100 mg of 30% ethanol was added and the mixture was dried at 60 캜 to form granules, which were then filled in a capsule.

Preparation Example 3 Preparation of anti-aging health food containing mastisia alboria extract or its fraction as an active ingredient

<3-1> Production of flour food

0.5 to 5.0 parts by weight of the mastisia alboria extract of the present invention or its fractions were added to wheat flour and the mixture was used to prepare bread, cake, cookies, crackers and noodles.

<3-2> Manufacture of soups and gravies

0.1 to 5.0 parts by weight of the mastisia alboria extract of the present invention or its fractions were added to soups and juices to prepare health-promoting meat products, noodles and juices.

<3-3> Preparation of ground beef

10 parts by weight of the mastisia alboria extract of the present invention or its fraction was added to ground beef to prepare ground beef for health promotion.

<3-4> Manufacture of dairy products

5 to 10 parts by weight of a mastisia alboria extract of the present invention or a fraction thereof was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

&Lt; 3-5 >

Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.

Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a conventional method, and then they were prepared into powder having a particle size of 60 mesh by a pulverizer.

The mastisia alboria extract of the present invention or its fraction was concentrated under reduced pressure in a vacuum concentrator, sprayed and dried with a hot air drier, and the resulting dried product was pulverized to a size of 60 mesh with a pulverizer to obtain a dried powder.

The grains, seeds and the mastisia albaoria extract of the present invention or fractions thereof were prepared in the following proportions:

(30 parts by weight of brown rice, 15 parts by weight of yulmu, 20 parts by weight of barley)

Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)

(3 parts by weight) of the mastisia alboria extract of the present invention,

(0.5 part by weight),

Rhubarb (0.5 parts by weight).

Preparation Example 4 Preparation of anti-aging beverage containing mastisia alboria extract or its fraction as an active ingredient

<4-1> Production of health drinks

5 g of the mastisia alboria extract or fraction thereof of the present invention is added to a homogeneous mixture such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5% And sterilized at the moment, and then packaged in small containers such as glass bottles and plastic bottles.

<4-2> Production of vegetable juice

Vegetable juice was prepared by adding 5 g of the mastisia alboria extract of the present invention or a fraction thereof to 1,000 ml of tomato or carrot juice.

<4-3> Preparation of fruit juice

A fruit juice was prepared by adding 1 g of the mastisia alboria extract of the present invention or a fraction thereof to 1,000 ml of apple or grape juice.

Claims (11)

An antioxidant cosmetic composition comprising an extract of Mastixia arborea CBClarke or a fraction thereof as an active ingredient.
The antioxidative cosmetic composition according to claim 1, wherein the mastisia alboria extract is extracted using water, a C ₁ to C ₂ lower alcohol, or a mixture thereof as a solvent.
The antioxidative cosmetic composition according to claim 2, wherein the lower alcohol is ethanol or methanol.
The antioxidative cosmetic composition according to claim 1, wherein the fraction is a fraction obtained by adding hexane, chloroform, ethyl acetate or butanol to the mastisia alboria extract.
A pharmaceutical composition for antioxidant comprising an extract of mastisia alboria or a fraction thereof as an active ingredient.
The method of claim 5, wherein the cyano maseuti al boria extract is water, C ₁ ~ C ₂ lower alcohol or antioxidant pharmaceutical composition characterized in that the extraction by using a mixture thereof as a solvent.
7. The antioxidant pharmaceutical composition according to claim 6, wherein the lower alcohol is ethanol or methanol.
[Claim 5] The antioxidant pharmaceutical composition according to claim 5, wherein the fraction is a fraction obtained by adding hexane, chloroform, ethyl acetate or butanol to the mastisia alboria extract.
An antioxidant health food comprising an extract of mastisia alboria or a fraction thereof as an active ingredient.
10. The antioxidant health food according to claim 9, wherein the mastisia alboria extract is extracted using water, methanol or a mixture thereof as a solvent.
10. The antioxidant health food according to claim 9, wherein the fraction is a fraction obtained by adding hexane, chloroform, ethyl acetate or butanol to the mastisia alboria extract.

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