KR101447007B1 - Composition for antiaging comprising the extract of Dipterocarpus obtusifolius Teijsm. Ex Miq. as an active ingredient - Google Patents

Abstract

The present invention relates to a Dipterocarpus < RTI ID = 0.0 > obtusifolius Teijsm. Ex Miq . ) Extract as an active ingredient. More specifically, the Dipterocarpus optic plant extract of the present invention has an effect of inhibiting DPPH free radical formation, an ABTS free radical generation inhibiting effect, and a superoxide radical Inhibitory effect and has an excellent antioxidative effect, it can be usefully used as a raw material for anti-aging medicines, health foods or cosmetics.

Description

[0001] The present invention relates to an antioxidant composition comprising an extract of Dipterocarpus optosporia as an active ingredient. Ex Miq. as an active ingredient}

The present invention relates to a Dipterocarpus < RTI ID = 0.0 > obtusifolius Teijsm. Ex Miq.) Extract as an active ingredient.

Free radicals that cause aging and disease in humans are known to form in biological reactions during normal metabolic processes in the body, causing harmful toxins to cells and tissues and causing various diseases (Folin O & Denis W, 1912, J Biol Chem 12: 239-249). Physiological actions that inhibit these harmful free radicals include electron donating ability to inhibit oxidation by donating electrons to oxidizing free radicals and superoxide anion radicals having a highly reactive and disruptive role similar to that of superoxide dismutase superoxide anion radical) into normal oxygen.

Several physiologically active ingredients with strong antioxidant effects contribute to prevention of ulcers and seizures caused by oxidative stress, regulation of gastric secretion and prevention of diarrhea, prevention of diabetes, prevention and reduction of cancer, heart disease and degenerative diseases (Azuma K et al., 1999, J Agric Food Chem 47: 3963-3966, Ham SS et al., 1997, J Food Sci Nutr 2: 155-161). Recently, interest in health has been increased, and resources containing a large amount of functional substances having various effects such as anticancer, antiallergic, anti-obesity, antioxidant and antibacterial effects have been selected from various plant resources, Kim et al., 2006, Food Sci Biotechnol., 15: 357-362, Park et al., 2004, Korean J Nutr, 37: 364-372, Kim et al., 2006, Kor J Food Sci. Technol. 38: 799-804, Ali KA et al., 2005, Food Chem., 89: 27-36).

Superoxide dismutase, an antioxidant, breaks down the active oxygen generated by the respiration process of cells, ie, superoxide radicals into hydrogen peroxide and oxygen. Hydrogen peroxide reacts with metal ions such as iron or copper ions to generate reactive oxygen radicals, which are stronger hydroxyl radicals. Before hydrogen peroxide reacts with metal ions, the antioxidants catalase and glutathione peroxidase degrade hydrogen peroxide into water and carbon dioxide. If the antioxidant that removes hydrogen peroxide does not completely remove 5% of hydrogen peroxide, it reacts to metal ions in the body and turns into a hydroxy radical, aging our body and causing illness. Frequent exposure to radiation or ultraviolet light can turn hydrogen peroxide into hydroxy radicals to promote senescence, and there is a method of taking antioxidants to delay it.

Superoxide radicals (O ^{2 -} ) themselves are relatively weak in reactivity, but they are easily converted to H ₂ O ₂ , resulting in hydroxyl radicals that are highly reactive or react with nitric oxide (NO) (Peroxynitrite) (ONOO ^- ), which causes the oxidation of the SH group, nitrification of protein tyrosine, lipid peroxidation, and DNA damage.

Antioxidants are widely distributed in copper and plants. Many antioxidants such as phenolic compounds, flavonoids, tocopherols, vitamin C and selenium in fruits and vegetables can delay or prevent the oxidation of fats and can cause cancer, cardiovascular diseases It also plays an important role in prevention of aging by preventing or delaying it. Tocopherol and vitamin C, which are natural antioxidants in the vegetable field, are widely used in foods, medicines and cosmetics. The rancidity of foods containing oil or fat is mainly caused by binding with oxygen in the air. To prevent this, many synthetic or natural antioxidants have been developed. However, synthetic antioxidants are used in practice because of their effectiveness and economy. Examples of synthetic antioxidants include Butylated Hydroxy Toluene (BHT), Butylated Hydroxy Anisole (BHA), and Propyl Gallate. Synthetic antioxidants are widely used in commercial foods because of their excellent antioxidant ability and low cost, but their use is regulated due to concerns about safety, such as human side effects. Natural antioxidants such as tocopherol are safe for the human body, but they have the disadvantage that they have low ability to inhibit the oxidative chain reaction and are expensive. Therefore, in recent years, researches for the development of natural antioxidants which are more safe and have excellent antioxidative effects have been actively conducted.

Dipterocarpus Optosporius belongs to the Dipterocarpaceae family, with 17 genera and over 500 species. It is distributed in India, Burma, Thailand, Cambodia, Vietnam and Laos. Wood is used for general construction. (WO 2006137122) reported that 70% ethanol extract of the above plant inhibited collagenase by 100%, and thus, it is possible to use other plant extracts Cream, shampoo, toothpaste and the like (JP 2010018546). However, there has been no report on the antioxidative or whitening effect and cell proliferation effect of Dipterocarpus optospores extract.

Thus, the inventors of the present invention have found that when plants having excellent antioxidative effects are screened, Dipterocarpus obtusifolius Teijsm. Ex Miq.) Extract showed strong antioxidative activity, the present inventors have completed the present invention by showing that it can be effectively used as an active ingredient of the composition for preventing aging.

It is an object of the present invention to provide an anti-aging pharmaceutical composition containing an extract of Dipterocarpus optima sp. As an active ingredient.

Another object of the present invention is to provide an anti-aging health food composition comprising an extract of Dipterocarpus optima sp. As an active ingredient.

It is still another object of the present invention to provide an anti-aging cosmetic composition containing an extract of Dipterocarpus optima sp. As an active ingredient.

In order to achieve the above object, the present invention provides a pharmaceutical composition for preventing aging comprising an extract of Dipterocarpus optic plant as an active ingredient.

In addition, the present invention provides an anti-aging health food composition comprising an extract of Dipterocarpus optima sp. As an active ingredient.

Further, the present invention provides an anti-aging cosmetic composition comprising an extract of Dipterocarpus optima sp. As an active ingredient.

The extract of the present invention extracted from Dipterocarpus optima plantarius showed an excellent antioxidative effect by showing DPPH free radical generation inhibitory effect, ABTS free radical generation inhibitory effect and superoxide radical inhibitory effect in a concentration dependent manner, And can be usefully used as a raw material for pharmaceuticals for prevention, health food or cosmetics.

FIG. 1 is a graph showing free radical scavenging effect of Dipterocarpus optospores extract according to an embodiment of the present invention.
2 shows the results of ABTS scavenging activity of Dipterocarpus optospores extract according to an embodiment of the present invention.
FIG. 3 shows the result of measurement of superoxide dismutase (SOD) activity of the extract of Dipterocarpus optosporus according to an embodiment of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention provides an anti-aging pharmaceutical composition containing a Dipterocarpus optosporus extract as an active ingredient.

In addition, the present invention provides a pharmaceutical composition for antioxidant containing an extract of Dipterocarpus optosporia as an active ingredient.

In the pharmaceutical composition according to the present invention, it is preferable to use the extract obtained by extracting the dried Dipterocarpus optiosporus with water, a C ₁ -C ₄ lower alcohol or a mixed solvent thereof.

The Dipterocarpus optic plant used in the present invention may be any of its root, stem and leaf parts, but preferably a stem part can be used.

As the C ₁ -C ₄ lower alcohol, methanol or ethanol is preferably used, and methanol is more preferably used.

Hereinafter, a method of extracting the Dipterocarpus optosporus extract according to the present invention will be described in detail.

The Dipterocarpus optosporus extract may be prepared by a process comprising the steps of:

A step of adding an extraction solvent to the Dipterocarpus optiosporus to extract (step 1);

Cooling the extract of step 1 and filtering (step 2); And

The step of extracting the filtered extract of step 1 and concentrating it under reduced pressure (step 3).

In the above method, the Dipterocarpus optima species of step 1 may be cultivated or commercially available. The Dipterocarpus optima species may be used in any of its roots, stems and leaves, Can be used.

The extraction solvent in step 1 is preferably water, a C ₁ -C ₄ lower alcohol or a mixture thereof, and the lower alcohol is preferably ethanol or methanol.

Examples of the extraction method include filtration, hot water extraction, immersion extraction, reflux cooling extraction, ultrasonic extraction, and the like, and extraction is preferably performed once to five times by hot water extraction, But is not limited thereto.

The extraction solvent may be added in an amount of 1 to 10 times, preferably 2 to 3 times, based on the weight of the dried dipterocarpus optocoupler.

The extraction temperature is preferably 20 to 100 ° C, but is not limited thereto. In addition, the extraction time is preferably 10 to 48 hours, more preferably 15 to 30 hours, but is not limited thereto.

In the above method, the vacuum concentration in step 3 may be carried out using a vacuum decompression concentrator or a vacuum rotary evaporator, and the drying may be carried out under reduced pressure, vacuum drying, boiling, spray drying or freeze drying.

In order to confirm the antioxidant effect of the extract of Dipterocarpus optic plant, the inventors of the present invention found that the DPPH free radical scavenging effect was induced by 1,1-diphenyl-2-picryl- The concentrations of the extracts were 9.84, 17.31, 29.84, 49.22, and 75.03 at concentrations of 1.3, 2.5, 5, 10, 20, 40, 80 and 160 ㎍ / ㎖ of Dipterocarpus optospores extract, respectively. , 84.46, 86.42, and 90.67%, respectively (see FIG. 1).

Further, in order to confirm the antioxidative effect of the extract of Dipterocarpus optorum cepificus, the present inventors measured the ABTS free radical scavenging effect according to various concentrations of the Dipterocarpus optosporia extract, and as a result, It was confirmed that ABTS free radical scavenging effect of 12.69, 23.51, 47.85, 63.24, 90.24, 100.09 and 100.18% was observed at the concentrations of 2.5, 5, 10, 20, 40, 80 and 160 ㎍ / 2).

Further, in order to confirm the SOD (Superoxide Dismutase) activity of the Dipterocarpus optospores extract, the present inventors measured the effect of superoxide radical scavenging by concentration of the Dipterocarpus optosporus extract on the SOD kit , And exhibited remarkable bubble oxide radical scavenging effects of 28.72, 43.13, 56.97, 69.02 and 74.73% at concentrations of 2.5, 5, 10, 20 and 40 / / ml of Dipterocarpus optospores extract (see FIG. 3) .

Therefore, the Dipterocarpus optosporus extract of the present invention exhibits DPPH free radical scavenging effect and ABTS free radical scavenging effect depending on the concentration for antioxidant, exhibits a remarkable superoxide radical scavenging effect and has an excellent antioxidative effect, It can be usefully used as an active ingredient of an active ingredient composition, a health food composition, and a cosmetic composition.

When the composition of the present invention is used in medicine, it may contain one or more active ingredients showing the same or similar functions in addition to the Dipterocarpus optospores extract.

The composition of the present invention may be administered orally or parenterally in various clinical formulations. In the case of formulation, a diluent or excipient such as a filler, a weight agent, a binder, a wetting agent, a disintegrant, ≪ / RTI > Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient, such as starch, calcium carbonate, gelatin It is quiet by mixing. Liquid preparations for oral administration include suspensions, solutions, emulsions, syrups and the like. Various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included in addition to water and liquid paraffin, which are simple diluents commonly used. have. Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, and freeze-drying agents. Examples of non-aqueous and non-aqueous solutions include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like.

The dosage varies depending on the patient's body weight, age, sex, health condition, diet, administration time, administration method, excretion rate, and disease severity. The daily dose of the extract of Dipterocarpus optosporia according to the present invention is about 0.1 to 100 mg / kg, preferably 5 to 50 mg / kg, more preferably once a day or several times a day.

Further, the present invention provides an anti-aging health food composition comprising a Dipterocarpus optic plant extract as an active ingredient.

Furthermore, the present invention provides an antioxidant health food composition containing an extract of Dipterocarpus optosporia as an active ingredient.

In the health food composition according to the present invention, the extract obtained by the above-described extraction method may be used.

The composition according to the present invention may be added to health supplements such as foods, beverages, etc. for the purpose of antioxidative action.

There is no particular limitation on the kind of the food. Examples of the foods to which the above substances can be added include dairy products including dairy products, meat, sausage, bread, biscuits, rice cakes, chocolate, candies, snacks, confectionery, pizza, ramen and other noodles, gums, ice cream, Beverages, alcoholic beverages and vitamin complexes, dairy products, and dairy products, all of which include health functional foods in a conventional sense.

The Dipterocarpus optiosporus extract of the present invention can be added directly to food or used together with other food or food ingredients, and can be suitably used according to conventional methods. The amount of the active ingredient to be mixed can be suitably determined according to its use purpose (for prevention or improvement). Generally, the amount of the extract in the health food may be 0.1 to 90 parts by weight of the whole food. However, in the case of long-term intake intended for health and hygiene purposes or for the purpose of controlling health, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount exceeding the above range.

The health functional beverage composition of the present invention has no particular limitation on the other ingredients other than the above-mentioned extract as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin and the like, and sugar alcohols such as xylitol, sorbitol and erythritol. Natural flavors (tau martin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.) and synthetic flavors (saccharin, aspartame, etc.) can be advantageously used as flavors other than those described above The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably about 5 to 12 g per 100 of the composition of the present invention.

In addition to the above, the Dipterocarpus optosporus extract of the present invention can be used as a flavoring agent such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and thickening agents (cheese, chocolate, etc.) Alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the Dipterocarpus optosporulus extract of the present invention may contain natural fruit juice and pulp for the production of fruit juice drinks and vegetable drinks.

These components may be used independently or in combination. The proportion of such additives is not so critical, but is generally selected in the range of from 0.1 to about 20 parts by weight per 100 parts by weight of Dipterocarpus optosporus extract.

The present invention also provides an anti-aging cosmetic composition comprising a Dipterocarpus optosporus extract as an active ingredient.

Furthermore, the present invention provides a cosmetic composition for antioxidation containing an extract of Dipterocarpus optosporia as an active ingredient.

In the cosmetic composition according to the present invention, the extract obtained by the above-described extraction method may be used.

The extract of Dipterocarpus optosporia according to the present invention can be used in a cosmetic composition for antioxidation, wherein the antioxidant cosmetics include, but are not limited to, lotions, ointments, gels, creams, patches or sprays . In preparing the antioxidative cosmetic composition containing the Dipterocarpus optima cepheuris extract of the present invention, the Dipterocarpus optic plant extract of the present invention is preferably added in an amount of 1 to 15 parts by weight, May be added in an amount of 2 or 10 parts by weight. The external composition for skin of the present invention may further contain, in addition to the Dipterocarpus optima sphiori extract of the present invention, a lipid, an organic solvent, a solubilizer, a thickening agent and a gelling agent, a softener, an antioxidant, a suspending agent, a stabilizer, , A perfume, a surfactant, water, an ionic or nonionic emulsifier, a filler, a sequestering and chelating agent, a preservative, a vitamin, a barrier, a wetting agent, a essential oil, a dye, a pigment, a hydrophilic or lipophilic active agent, Or any other ingredient conventionally used in external preparations for dermatological purposes. The components can also be introduced in amounts commonly used in the field of dermatology.

Hereinafter, the present invention will be described by way of examples.

However, the following examples are illustrative of the present invention, and the contents of the present invention are not limited to the following examples.

< Example  1> Dipterocarpus Optor Shipyards  Preparation of extract

20 mg of Dipterocarpus optosporium methanol extract was purchased from the International Biomaterials Hub Center, Korea Research Institute of Bioscience and Biotechnology.

< Experimental Example  1> Dipterocarpus Optor Shipyards  Extract DPPH  freedom Radical  Measurement of scavenging activity

The free radical scavenging activity of the Dipterocarpus optosporia extract obtained in Example 1 was measured using 1,1-diphenyl-2-picryl-hydrazyl, DPPH, Sigma) method. For the measurement of free radical scavenging activity, 90 μl of a 0.15 mM DPPH solution well dissolved in ethanol was placed in a 96-well plate and the extract was divided into 2.5, 5, 10, 20, 40 and 80 μg / ml, After leaving for a minute, the absorbance was measured at 517 nm with a microplate meter. The free radical scavenging activity (%) was calculated by the following equation (1). At this time, the group treated with DMSO was used as a negative control, and the group treated with BHA as a synthetic antioxidant was used as a positive control.

(In the above formula (1)

A: Absorbance of the negative control not treated with the extract of the present invention

B: Absorbance of the test group treated with the extract of the present invention.

As a result, DPPH free radical scavenging effect on Dipterocarpus optospores extract was measured as shown in FIG. 1. As a result, it was found that the Dipterocarpus optosporium extract extracts of 1.3, 2.5, 5, 10, 20, 40 , 80.4 and 160 ㎍ / ㎖ of DPPH radical scavenging activity of 9.84, 17.31, 29.84, 49.22, 75.03, 84.46, 86.42 and 90.67%, respectively, and showed similar activity to BHA as a positive control group 1).

< Experimental Example  2> Dipterocarpus Optor Shipyards  Extract ABTS  freedom Radical  Measurement of scavenging activity

The ABTS radical scavenging activity was obtained by mixing ABTS and potassium persulfate to a final concentration of 7 mM and 2.45 mM, respectively, and then left in the dark for 12 hours to form ABTS radicals. This was diluted with distilled water to an absorbance of 0.7 at 734 nm and dispensed into a 96 well plate. The sample was dispensed at concentrations of 2.5, 5, 10, 20, 40, 80 and 160 / / And reacted in a cow for 7 minutes. Absorbance was measured using a microplate meter at 734 nm. At this time, the group treated with DMSO was used as a negative control, and the group treated with BHA as a synthetic antioxidant was used as a positive control.

As a result, ABTS free radical scavenging effect on the extract of Dipterocarpus optosporifolia was measured as shown in FIG. 2, and as a result, Dipterocarpus optosporus extract 2.5, 5, 10, 20, 40, 80 and 160 (Fig. 2) at a concentration of 10 &lt; 6 &gt; / mu g / ml and 12.69, 23.51, 47.85, 63.24, 90.24, 100.09 and 100.18%, respectively.

< Experimental Example  3> Dipterocarpus Optor Shipyards  Extract SOD  Active measurement

In order to confirm the antioxidative activity of the extract obtained in Example 1, the scavenging activity of the superoxide radical acting as a precursor of other harmful active oxygen species was measured. The superoxide radical scavenging activity can be detected by measuring the activity of superoxide dismutase (SOD), which is an enzyme that cleaves superoxide by dismutting superoxide. In the present invention, SOD assay kit of Dojindo Respectively. Specifically, samples of each concentration were dispensed into a 96-well plate, and water-soluble WST-1 (2- (4-indophenyl) -3- (4-nitrophenyl) -5- -Dispephenyl) -2H-tetrazolium) was added thereto, and the SOD enzyme solution was added thereto, followed by reaction at 37 ° C for 20 minutes, and the absorbance of the reaction solution was measured at 450 nm with a microplate meter. The superoxide radical scavenging activity of each sample was also calculated by the reduction reduction of WST for the control group, and the concentration of the sample to which 50% of the superoxide radical was scavenged was defined as IC ₅₀ . At this time, the group treated with DMSO was used as a negative control, and the group treated with BHA as a synthetic antioxidant was used as a positive control.

As a result, the superoxide radical scavenging effect on the Dipterocarpus optospores extract was measured as shown in FIG. 3. As a result, the Dipterocarpus optospores extract at 2.5, 5, 10, 20 and 40 / / Ml inhibited the superoxide radical scavenging effect of 28.72, 43.13, 56.97, 69.02 and 74.73%, respectively, and the positive control group BHA inhibited the superoxide radical by 69.00 ± 0.88% at the concentration of 10 μg / ml, It was confirmed that the extract of Capersoptosporous was inhibited by 20.53 ± 1.03% at the same concentration, and it was confirmed that the activity was excellent (see FIG. 3).

Accordingly, the composition containing the extract of Dipterocarpus optosporia according to the present invention as an active ingredient exhibits excellent radical scavenging activity, and thus can be used for medicines, health foods, cosmetics and the like.

< Formulation example  1> Preparation of pharmaceutical preparations

1. Preparation of tablets

Tablets containing the extract of Dipterocarpus optospora sp. Of the present invention as an active ingredient were prepared as follows.

The compound of the above example was sieved, mixed with lactose, starch and pregelatinized corn starch, then added with a suitable volume of purified water and granulated with a powder. The granules were dried and then mixed with magnesium stearate and compressed to obtain tablets.

The components of the tablet are as follows.

Dipterocarpus optosporus extract 5.0 mg

Lactose BP 150.0 mg

Starch BP 30.0 mg

Total gelatinized corn starch BP 15.0 mg

Magnesium stearate 1.0 mg

2. Preparation of capsules

The capsules containing the Dipterocarpus optosporus extract of the present invention as an active ingredient were prepared as follows.

The compound of the above example was mixed with a certain amount of excipient and magnesium stearate. The resulting mixture was filled into gelatin capsules to obtain capsules.

The components of the capsules are as follows.

Dipterocarpus optosporus extract 5.0 mg

Starch 1500 100.0 mg

Magnesium stearate BP 1.0 mg

3. Preparation of injection solutions

An injection solution containing the Dipterocarpus optosporus extract of the present invention as an active ingredient was prepared by the following method.

The pH of the resulting solution was adjusted to pH 3.5 using dilute hydrochloric acid BP, followed by adjustment of the volume using injectable sodium chloride BP. &Lt; RTI ID = 0.0 &gt; And sufficiently mixed. The solution was filled in a 5 ml type I ampoule made of transparent glass, sealed in the upper lattice of the air by dissolving the glass, and autoclaved at 120 ° C for 15 minutes or longer to obtain an injection solution.

The components of the injection solution are as follows.

100 mu g / ml of Dipterocarpus optosporus extract

Until dilute hydrochloric acid BP pH 3.5

Sodium chloride BP injected up to 1 ml

< Formulation example  2> Dipterocarpus Optor Shipyards  Preparation of functional foods containing extracts

The inventors of the present invention prepared foods containing the above-mentioned Dipterocarpus optosporus extract as an active ingredient as follows.

1. Manufacture of beverages

Honey 522 mg

5 mg &lt; RTI ID = 0.0 &gt;

Nicotinic acid amide 10 mg

3 mg of sodium riboflavin hydrochloride

Pyridoxine hydrochloride 2 mg

Inositol 30 mg

Orthoic acid 50 mg

Dipterocarpus optosporus extract 0.48 to 1.28 mg

200 ml of water

A beverage was prepared using the above-mentioned composition and content by a conventional method.

2. Of chewing gum  Produce

Gum base 20%

Sugar 76.36 ~ 76.76%

Dipterocarpus optosporus extract 0.24 to 0.64%

Fruit flavor 1%

Water 2%

Chewing gum was prepared using the above-mentioned composition and content by a conventional method.

3. Manufacture of candy

Sugar 50 to 60%

Syrup 39.26 ~ 49.66%

Dipterocarpus optosporus extract 0.24 to 0.64%

Orange fragrance 0.1%

The composition and the content of the candy were prepared using a conventional method.

4. Biscuit  Produce

First class 88 ㎏ power

Gravity Class 1 76.4 kg

16.5 kg

Salt 2.5 ㎏

Glucose 2.7 kg

Palm shortening 40.5 kg

Ammonium 5.3 kg

0.6 kg

Sodium bisulfite 0.55 kg

Rice powder 5.0 kg

Vitamin B1 0.003 kg

Vitamin B2 0.003 kg

Milk flavor 0.16 kg

Water 71.1 kg

Whole milk powder 4 ㎏

1 kg of substitute milk powder

Calcium phosphate 0.1 kg

Spray salt 1 kg

Spray oil 25 kg

Dipterocarpus optosporus extract 0.2 to 0.5 kg

The biscuits were prepared using the above-mentioned composition and content by a conventional method.

5. Manufacture of ice cream

Fat milk 10.0%

Solid oil content 10.8%

Sugar 12.0%

Starch syrup 3.0%

Emulsion stabilizer (span) 0.5%

Perfume (Strawberry) 0.15%

Water 63.31 ~ 62.91%

Dipterocarpus optosporus extract 0.24 to 0.64%

Ice cream was prepared using the above-mentioned composition and content by a conventional method.

6. Of chocolate  Produce

Sugar 34.36 ~ 34.76%

Cocoa Butter 34%

Cocoa mass 15%

Cocoa powder 15%

Lecithin 0.5%

Vanilla flavor 0.5%

Dipterocarpus optosporus extract 0.24 to 0.64%

The composition and the content thereof were used to prepare chocolate by a conventional method.

< Formulation example  3> Preparation of external preparation for skin

1. Manufacture of cream

Cetostearyl alcohol 2.8 parts by weight

Beeswax 2.6 parts by weight

Stearic acid 1.4 parts by weight

Glycerin monostearate 2 parts by weight

&Lt; tb &gt; &lt; tb &gt;

Sorbitol sesquioleate 1.4 parts by weight

Jojoba oil 4 parts by weight

Squalane 3.8 parts by weight

Polysorbate 60 1.1 parts by weight

Macadia oil 2 parts by weight

Tocopheryl acetate 0.2 part by weight

Methylpolysiloxane 0.4 part by weight

Ethylparaben 0.1 part by weight

Propylparaben 0.1 part by weight

Euxyl K-400 0.1 part by weight

1,3-butylene glycol 7 parts by weight

Methylparaben 0.05 part by weight

Glycerin 6 parts by weight

d-Pandenol 0.2 part by weight

Dipterocarpus optosporus extract 4.6 parts by weight

Triethanolamine 0.2 part by weight

pt 41891 0.2 part by weight

p-H20 46.05 parts by weight

2. Manufacture of Lotion

Cetostearyl alcohol 1.6 parts by weight

Stearic acid 1.4 parts by weight

Glycerin monostearate of pro-type 1.8 parts by weight

&Lt; tb &gt; &lt; tb &gt;

Sorbitol sesquioleate 0.6 parts by weight

Squalene 4.8 parts by weight

Macadia oil 2 parts by weight

Jojoba oil 2 parts by weight

Tocopheryl acetate 0.4 part by weight

0.2 part by weight of methylpolysiloxane

Ethylparaben 0.1 part by weight

Propylparaben 0.1 part by weight

4 parts by weight of 1,3-butylene glycol

0.1 part by weight of methylparaben

Xanthan gum 0.1 part by weight

Glycerin 4 parts by weight

d-Pandenol 0.15 part by weight

Allantoin 0.1 part by weight

Dipterocarpus optosporus extract 3.5 parts by weight

Cargar (2% aq. Sol) 4 parts by weight

Triethanolamine 0.15 part by weight

Ethanol 3 parts by weight

pt 41891 0.1 part by weight

p-H20 48.3 parts by weight

3. Manufacture of shampoo

The shampoo composition of the present invention was prepared using the following formulation.

Sodium laureth-7-sulfate 16 parts by weight

Lauroyl diethanolamide 2.0 parts by weight

Dipterocarpus optosporous extract 0.5 part by weight

DI water (DI water) 81.5 parts by weight

4. Manufacture of rinse

The rinse composition of the present invention was prepared using the following formulation.

Barrico Art 638 2.0 parts by weight

Lauroyl diethanolamide 2.0 parts by weight

Silicone oil (molecular weight 10,000) 0.5 part by weight

Dipterocarpus optosporous extract 0.5 part by weight

DI water (DI water) 95.0 parts by weight

5. Manufacture of hair oil

The hair oil composition of the present invention was prepared using the following formulation.

Octamethylcyclotetrasiloxane 40.0 parts by weight

Dipterocarpus optosporus extract 5.0 parts by weight

Anhydrous ethanol 55.0 parts by weight

6. Manufacture of hair spray

The hair spray composition of the present invention was prepared as follows.

Dipterocarpus optosporus extract 5.0 parts by weight

Ethyl alcohol 95.0 parts by weight

Claims (9)

A pharmaceutical composition for antioxidation comprising as an active ingredient at least one extract selected from the group consisting of roots, stems and leaves of Dipterocarpus opt.
The antioxidative pharmaceutical composition according to claim 1, wherein the Dipterocarpus optosporus extract is extracted using water, a C ₁ to C ₄ lower alcohol or a mixture thereof as a solvent.
3. The antioxidant pharmaceutical composition according to claim 2, wherein the lower alcohol is methanol or ethanol.
A health food composition for antioxidation comprising, as an active ingredient, one or more extracts selected from the group consisting of roots, stems and leaves of Dipterocarpus optiosporus.
The method of claim 4, wherein the deep Tero Kuipers opteo sipori ground extract is water, C ₁ ~ C ₄ lower alcohol or antioxidant composition for health food characterized in that the extraction by using as the solvent a mixture thereof.
The composition according to claim 5, wherein the lower alcohol is methanol or ethanol.
A cosmetic composition for antioxidation comprising, as an active ingredient, any one or more extracts selected from the group consisting of roots, stems and leaves of Dipterocarpus optiosporus.
The antioxidative cosmetic composition according to claim 7, wherein the Dipterocarpus optosporus extract is extracted with water, a C ₁ -C ₄ lower alcohol or a mixture thereof as a solvent.
The antioxidative cosmetic composition according to claim 8, wherein the lower alcohol is methanol or ethanol.

Images