KR101936294B1 - Composition comprising for skin-whitening and anti-wrinkling extract of Rumex acetosella L. or extract of Hydrangea serrata - Google Patents

Abstract

The present invention relates to a composition for improving wrinkles or for whitening skin, which contains an extract of Swine Swine or mountain juice as an active ingredient.
According to the present invention, the composition of the present invention can exhibit skin wrinkle improvement or skin whitening effect by inhibiting tyrosinase or elastase, and as a safe material, Can be usefully used.

Description

[0001] The present invention relates to a composition for improving wrinkles or skin whitening comprising an extract of Rumex acetosella L. or extract of Hydrangea serrata,

The present invention relates to a composition for improving wrinkles or skin whitening, and more particularly to a composition for improving wrinkles or skin whitening, which contains an extract of Aji swim or mountain water soup as an active ingredient.

As the elderly population increases, the beauty of the skin, which can be a measure of aging, and the antioxidant that can prevent aging are becoming a major concern. The generation of wrinkles of the skin due to aging and the reduction of antioxidant activity in the body can be used as a measure of health. Skin aging can be classified into intrinsic aging and extrinsic aging depending on factors. Endogenous aging is caused by changes in physiological functions of skin epidermis and dermis according to age. Exogenous aging is known to be caused by changes in physiological functions of skin caused by environmental pollution, exposure to ultraviolet rays, and stress. The common mechanism of this aging is to increase the free radicals in the body by continuously being exposed to oxidative stress and to increase the activity of Elastase which breaks down the connective tissue of the dermis, Elastin , Degradation of hyaluronic acid (Hyaluronic acid) can be explained by increased activity of hyaluronidase. In other words, skin aging can be explained by the collapse of elastin and hyaluronic acid which are basic structures of skin, collapsing the dermis, making skin dry, and wrinkles. In addition, radicals in the body are involved in the melanin formation process, thereby activating tyrosinase, which causes spots and freckles.

In everyday life, we are always exposed to the hazards caused by active oxygen, the aging hazards accumulate gradually, and the antioxidant capacity is gradually reduced. Under normal physical conditions, the antioxidant protection system removes reactive oxygen species through enzymatic (superoxide dismutase, etc.) and non-enzyme (polyphenols, flavonoids, etc.) pathways. Therefore, in order to exhibit antioxidant activity, it is necessary to activate the antioxidant enzyme reaction such as SOD or to take a non-enzyme substance which has a direct antioxidant effect.

Therefore, research on the mechanism of skin health and antioxidation has been actively conducted, and researches on cosmetics and health functional foods showing biochemical efficacy and efficacy have been conducted. The main research subjects are materials with practical effects and effects due to the development of research level and the increase of technology.

Plant-derived materials have been used for a long time because of their excellent safety, and in particular, functional materials based on plant and herbal ingredients used in the private sector or used in oriental medicine are actively developed.

Hydrangea serrata is a broad-leaved tall tree of the submandibular gland, and its leaf parts are indicated as edible parts in food raw materials notified in the place of shipment. It is used as a treatment for chronic bronchitis, warts, genomes, and anti-inflammatory drugs. Korean Patent Laid-Open Publication No. 2004-0063874 discloses a composition containing an extract of Oncorhynchospora having hyperglycemia and hyperlipemia inhibitory activity.

Swim swimming ( Rumex Acetosella L. ) is a plant belonging to the genus Pseudomonas spp., which is indicated as a possible food source in the raw materials of foodstuffs notified by the pet food. The leaves and stems of the leaves are used as medicinal materials and mainly used for anticancer, antioxidant, diuretic, (Use of herbal ingredients and use, Jan., 1994). Korean Patent Laid-Open Publication No. 2005-0056569 discloses a cosmetic for improving atopic skin based on anti-inflammatory, antioxidant, and antibacterial action of swine swimming.

However, there is no direct research on the mechanism of skin wrinkle improvement or skin whitening effect in swine swimming or mountain sowing. Therefore, the inventors of the present invention conducted a study on the direct basic effects of the extracts on skin wrinkles or skin whitening.

Thus, the present inventors have made research efforts result, baby swim (Rumex to overcome the problems of the prior art acetosella L. ) or hydrangea serrata as an active ingredient, it may exhibit skin wrinkle improvement or skin whitening effect by inhibiting tyrosinase or elastase. And the present invention was completed.

KR 10-2004-0063874 A KR 10-2005-0056569 A

 WOO, M. S., et al. Comparison of skin elasticity test results from the Ballistometer® and Cutometer®. Skin Research and Technology, 2014, 20.4: 422-428.  IMOKAWA, Genji. Mechanism of UVB-induced wrinkling of the skin: paracrine cytokine linkage between keratinocytes and fibroblasts leading to the stimulation of elastase. In: Journal of Investigative Dermatology Symposium Proceedings. Nature Publishing Group, 2009. p. 36-43.

Accordingly, it is a main object of the present invention to provide a composition for improving wrinkles or skin whitening comprising an effective amount of an extract of a swine swimming or mountainous soup having skin wrinkle improvement or skin whitening effect by inhibiting elastase or tyrosinase There is.

According to one aspect of the invention there is provided a baby swim (Rumex acetosella L. ) or an extract of Hydrangea serrata as an active ingredient.

As the elderly population increases, the beauty of the skin, which can be a measure of aging, is becoming a major concern. In particular, the interest in wrinkle improvement and skin whitening has been increasing steadily, and many health functional foods and cosmetics have been actively researched in order to improve them, and their products are being widely launched. However, since health functional foods and cosmetics are directly ingested and absorbed into the skin, safety is also an important issue. Therefore, plant-derived materials superior in safety are being steadily used. Accordingly, the inventors of the present invention have confirmed that the baby swim extract or the mountain water extract of the natural plant-derived material is excellent in skin wrinkle improvement or skin whitening effect, and the present invention has been completed.

In the present invention, the extract can be extracted with any solvent conventionally used for extracting natural plants, preferably water, fermented juice or a mixed solvent thereof, more preferably extracted with ethanol .

In the present invention, the composition may be contained in an amount of 0.001 to 80.0% by weight, and preferably 5 to 20% by weight based on the total weight of the composition. Also, it was confirmed that the above conditions exhibited a high yield and excellent enzyme inhibitory effect.

In the present invention, the composition is characterized by exhibiting an effect of improving wrinkles by inhibiting elastase.

According to the experimental examples of the present invention, it was confirmed that the inhibition rate of the elastase activity in the case of the baby swimming extract or the extract of the acidophilus extract of the present invention showed a similar effect to that of the positive control adempsin (Adempsin) Given that it is an extract that is not a substance, we can confirm that the figure is never low. From these results, it can be seen that the composition of the present invention can improve the appearance of wrinkles of the skin by inhibiting elastase which degrades elastin, which is a connective tissue of dermis (see Experimental Example 2).

In the present invention, the composition is characterized by exhibiting skin whitening effect by inhibiting tyrosinase.

According to the experimental examples of the present invention, it was confirmed that the inhibitory activity of tyrosinase activity in the case of the Ajiwa swimming extract or the extract of Sansho sprout of the present invention showed a similar effect to that of Arbutin, which is a positive control group, In view of these facts, the present invention relates to a composition for inhibiting melanin production, which is an enzyme involved in the production of melanin, which causes pigmentation, of tyrosinase It is possible to show a skin whitening effect by inhibiting the activity (see Experimental Example 1).

In the present invention, the composition has at least one form selected from the group consisting of tablets, granules, pills, capsules, liquids, jellies or gums, and is characterized in that it is for oral consumption.

In the present invention, the composition has at least one formulation selected from the group consisting of a softening agent, a nutritional lotion, a nutritional cream, a water cream, a spot, a gel lotion or an ointment.

As described above, the composition of the present invention containing the baby swimming extract or the extract of mountain juice extract inhibits elastase and tyrosinase, thereby exhibiting skin wrinkle improvement or skin whitening effect, Composition, and as a safe material, an extract of Swine Swine or Mountain Swine can be usefully used in the fields of food composition or cosmetics.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example 1: Preparation of an extract using ethanol

The baby swim and the extract of the mountaineering soup contained in the composition of the present invention are produced by the following process. First of all, the first year baby swimming and mountain juice native to Jeju Island was purchased, washed thoroughly with distilled water, and completely dried until there was no change in weight in a cool, sun-free place. Thereafter, the completely dried dried material was pulverized by using a homogenizer to obtain pulverized products, and 400 g of an extraction solvent was prepared by mixing ethanol and distilled water at a weight ratio of 7: 3, and then 50 g of each of the pulverized products was added. At this time, the mixing ratio of the pulverized material to the extraction solvent was 1: 8 by weight. Next, the extraction solvent mixed with the pulverized product was put into a shaking constant-temperature water bath and stirred at 50 DEG C for 2 hours. The material obtained by extraction was insoluble matter removed at room temperature using Wattman (No. 2) extraction solvent filter paper. Thereafter, the solvent was completely removed while concentrating under reduced pressure in a distillation apparatus equipped with a cooling condenser. The extracts were completely dried and their yields were measured. As a result of the measurement of yield, the swimming rate was 5.82% and the mountain water station was 20%.

Experimental Example 1: Inhibition of tyrosinase activity

The inhibitory effect of tyrosinase activity on the extract obtained in Example 1 was measured. In this experiment, tyrosinase was extracted from mushrooms (Sigma-Aldrich, US). The degree of color development based on melanin formation of the L-tyrosine substrate, which is a substrate for tyrosinase, was determined by measuring the absorbance at 490 nm.

To 120 μl of 0.1 M phosphate buffer (Phosphate buffer pH 6.8), 40 μl of 1.5 mM L-tyrosine was added to prepare a reaction solution. After adding 20 μl of the sample solution to the reaction solution and stabilizing for 5 minutes, 20 μl of mushroom tyrosinase (100 U / ml) was added and enzyme reaction was performed at 37 ° C for 10 minutes. In the control group, DMSO was added instead of the sample solution. DMSO and 0.1 M phosphate buffer solution were added to the blank sample instead of the sample solution. The reaction solution was added, and the reaction was allowed to proceed at 37 ° C for 10 minutes. The absorbance at 490 nm was measured and calculated according to the following equation (1) to determine the enzyme inhibitory activity of the sample. The results are shown in Table 1.

[Formula 1]

extract Inhibition rate of tyrosinase activity (%) 200 [mu] g / ml 100 mu g / ml 50 mu g / ml Mountain landscape 21.86 10.35 5.17 Baby swimming 24.50 18.37 9.19 Arbutin 29.50 21.30 10.20

As shown in the above Table 1, the tyrosinase activity inhibition test was carried out by treating the extract of Araliae sativa L. and Swine Swine by concentration, and it was confirmed that the effect was similar to that of arbutin, which is a positive control group. Considering this extract, the tyrosinase activity inhibitory effect was relatively high in a concentration-dependent manner. In addition, it has been confirmed that swine swimming can be used as a more effective composition for skin whitening because it exhibits a higher activity inhibitory effect than that of mountain syrup.

Experimental Example 2: Elastase activity inhibitory effect

The extract obtained in Example 1 was measured for the inhibitory effect of elastase activity. Elastase was extracted from the pig pancreas in this experiment (Sigma-Aldrich, US). N-succinyl- (Ala) 3-p-nitroaniline, which is a peptide substrate of elastase, is used to measure the increase in the amount of 4-nitroaniline produced per unit time by the activity of the elastase Respectively.

20 μl of 8.8 mM N-succinyl- (Ala) 3-p-nitroaniline was added to 140 μl of 0.276 M Trizma-HCl (pH 8.0) to prepare a reaction solution. 20 μl of the sample solution was added to the reaction solution, and after stabilization for 5 minutes, 20 μl of elastase (2.5 U / ml) was added and enzyme reaction was carried out at 37 ° C for 20 minutes. In the control group, DMSO was added instead of the sample solution. DMSO was used instead of the sample solution and BSA solution was added to the blank solution instead of the enzyme. After the reaction, the absorbance was measured at 410 nm, and the result was calculated according to the following equation (2) to determine the enzyme inhibition activity of the sample. The results are shown in Table 2.

[Formula 2]

extract Elastase activity inhibition (%) 200 [mu] g / ml 100 mu g / ml 50 mu g / ml Mountain landscape 11.57 11.01 6.07 Baby swimming 9.38 7.85 7.15 Adenosine 18.40 17.36 11.14

As shown in the results of Table 2, the extract of Araliaceae and Aigi Swim was treated by concentration to examine the activity of inhibiting elastase activity. As a result, it was confirmed that the effect was similar to that of adenosine, which is a positive control, Considering this extract, the efficacy of inhibiting elastase activity, which is relatively high in concentration, could be confirmed. In addition, it was confirmed that the mountaineering plant showed a higher activity inhibitory effect than the baby swimming, and thus it can be used as a composition more effective in inhibiting wrinkle formation.

Experimental Example 3: SOD activity effect

The SOD enzyme activity of the extract obtained in Example 1 was measured. In this experiment, SOD and Superoxide dismutase activities were measured by SOD Assay Kit (Sigma-Aldrich, US). The WST-Formazan (WST-Formazan), which is developed by the peroxide generated by the oxidation process, is measured at 420 nm. The enzymatic activities of SOD enzymes were determined by measuring the degree of color degradation due to the decrease in peroxides and the decrease in formation of formazan when the enzyme showed activity.

Twenty microliters of the sample solution was added to 160 占 퐇 of the WST reaction solution of the kit, and after stabilization for 5 minutes, 20 占 퐇 of the enzyme reaction solution was added and the enzyme reaction was carried out at 37 占 폚 for 30 minutes. In the control group, DMSO was added instead of the sample solution. DMSO and buffer instead of enzyme were added to the blank sample instead of the sample solution. The absorbance was measured at 420 nm after the reaction, and the result was calculated according to the following equation (3) to determine the degree of enzyme activity of the sample. The results are shown in Table 3.

[Formula 3]

extract SOD enzyme activity (%) 200 μg / ml 100 μg / ml 50 μg / ml Mountain landscape 97.64 90.45 75.98 Baby swimming 98.44 94.87 90.51

As shown in the above Table 3, SOD enzymatic activity tests were conducted on the extracts of mountain and southern flounder and swine swimming, and the activity of SOD enzymes was confirmed by SOD and swimming. As a result of the experiment using the kit, it was confirmed that the SOD enzyme activity was highly dependent on the concentration of the extract-treated control group. It was confirmed that swine swimming showed higher SOD activity effect than that of mountain syrup, and it can be used as an effective antioxidant composition by activating SOD enzyme at 90% at low treatment concentration (50 μg / ml).

Experimental Example 4: Analysis of total polyphenol content

To 20 μl of the extract dissolved at a concentration of 10 mg / ml were added 140 μl of 20% Folin Ciocalteau reagent and 10% sodium carbonate (Na ₂ CO ₃ ) solution, and 20 μl of dH ₂ O were added in order, After 30 minutes of reaction, the absorbance was measured at 765 nm. The polyphenol content in the sample was calculated by the following formula (4). The total polyphenol content was calculated from the calibration curve prepared by analyzing Gallic Acid in the same manner as the standard material. The results are shown in Table 4.

[Formula 4]

Experimental Example 5: Analysis of total flavonoid content

10 μl of 5% sodium nitrite solution (NaNO ₂ ) was added to 20 μl of the extract dissolved at a concentration of 10 mg / ml, reacted for 5 minutes, added with 100 μl of aluminum chloride solution (AlCl ₃₆ H ₂ O) and reacted again for 5 minutes. Thereafter, 50 μl of 1 M sodium hydroxide (NaOH) was mixed and reacted for 10 minutes, and the absorbance was measured at 510 nm. The content of flavonoid in the sample was calculated by the following formula (5). The total flavonoid content was calculated from the calibration curve prepared by analyzing catechin (catechin) in the same manner as the standard material. The results are shown in Table 4.

[Formula 5]

extract Total polyphenol content Total flavonoid content TPC (mg GAE / g) TFC (mg Cathechin / g) Mountain landscape 5.59 5.38 Baby swimming 7.41 10.00

Formulation Example 1: Preparation of tablets

The extracts obtained in Example 1 were mixed with the components shown in Table 5 according to a conventional tablet preparation method and tableted to prepare tablets.

Raw material name Unit weight  (mg) The extract of Example 1 50 Corn starch 100 Lactose 100 Stearic acid 2

Formulation Example  2: Preparation of capsules

The ingredients obtained in Example 1 were mixed with the ingredients shown in Table 6 according to a conventional capsule preparation method and filled in gelatin capsules to prepare capsules.

Raw material name Unit weight  (mg) The extract of Example 1 50 Corn starch 100 Lactose 100 Stearic acid 2

Formulation Example  3: Liquid  Produce

The ingredients obtained in Example 1 were mixed with the ingredients listed in Table 7 according to the preferred beverage preparation method, and filled into bottles or pouches to prepare a liquid preparation.

Raw material name Unit weight  (g) The extract of Example 1 2.5050 Xanthan gum 0.0075 Fructooligosaccharide solution 0.7500 Coconut flower essence powder 1.0500 Twin condensate 1.5000 Red ginseng 0.0450 Purified water 9.1425

Formulation Example 4: Preparation of jelly

The extracts obtained in Example 1 were mixed with the ingredients shown in Table 8 according to the preferred jelly preparation method, and the jellies were prepared by filling three kinds of jelly.

Raw material name Unit weight  (g) The extract of Example 1 2.0000 Food gel 0.3600 Carrageenan 0.0600 Calcium lactate 0.1000 Sodium citrate 0.0600 Composite Golden Extract 0.0200 Enzyme treatment Stevia 0.0440 Fructooligosaccharide solution 5.0000 Red grape concentrate 2.4000 Purified water 13.9560

Formulation Example 5: Preparation of nutritional cream

Nutritive creams were prepared for the extracts obtained in Example 1 according to the usual methods according to the composition shown in Table 9 below.

Raw material content The extract of Example 1 1.0 Sitosterol 4.0 Polyglyceryl 2-olate 3.0 3.0 Setares-4 2.0 cholesterol 3.0 Dicetyl phosphate 0.4 Concentrated glycerin 5.0 Sunflower oil 22.0 Carboxyvinyl polymer 0.5 Triethanolamine 0.5 antiseptic a very small amount Spices a very small amount Purified water Balance

Example 1 of the above formulation examples may be any one selected from a baby swimming extract, a mountain juice extract, or a mixed extract thereof.

In addition, although the above-mentioned composition ratio is generally prepared by mixing a suitable component, it is also possible to arbitrarily change the compounding ratio and the raw material as necessary.

The extract of the present invention was stable in all formulation test conditions and therefore had no problem in the stability of the formulation.

Claims (7)

A composition for improving wrinkles and skin whitening comprising Hydrangea serrata extract of water, ethanol or a mixed solvent thereof as an active ingredient.
delete The composition for improving wrinkles and skin whitening according to claim 1, wherein the composition comprises 0.001 to 80% by weight based on the total weight of the composition.
The composition according to claim 1, wherein the composition exhibits a wrinkle-reducing effect by inhibiting elastase.
The composition according to claim 1, wherein the composition exhibits a skin whitening effect by inhibiting tyrosinase.
The composition according to claim 1, wherein the composition has one or more formulations selected from the group consisting of tablets, granules, pills, capsules, liquids, jellies or gums, Composition.
The composition according to claim 1, wherein the composition has at least one formulation selected from the group consisting of softening water, nutritional lotion, nutritional cream, water cream, spot, gel or lotion, / RTI >