KR102401490B1 - Compositions containing Angelica gigas extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving skin whitening and blocking ultraviolet rays, and by containing an angelica asiatica ultrasound extract as an active ingredient, it has excellent antioxidant effect and is effective in improving skin whitening and blocking ultraviolet rays.

Description

Cosmetic composition for skin whitening improvement and UV protection containing Angelica gigas extract as an active ingredient {Compositions containing Angelica gigas extract}

The present invention relates to a cosmetic composition and a health functional food containing, as an active ingredient, an ultrasonic wave extract fermented with probiotics or not fermented.

As modern people are more interested in health, various studies on natural and eco-friendly cosmetics are being actively conducted. In functional cosmetics, interest in cosmetics using natural materials is increasing rather than in cases where chemical ingredients-based whitening, antioxidants, UV protection, preservatives, and antibacterial agents were used in the past.

Among the many natural materials, animal-derived materials are actively developing functional cosmetics using plant-derived ingredients due to increased consumer rejection of animal-derived materials due to animal welfare, mad cow disease, swine cholera, and bird flu. Consumer demand for multifunctional cosmetics such as wrinkle improvement and UV protection is increasing.

Functional cosmetics refer to cosmetics in various stages of use, such as essence, serum, cream, powder, and base, with specific functions added such as whitening, wrinkle improvement, and UV protection. Complex functional cosmetics with one function have also been developed to increase the ease of use, and products specialized for various purposes such as pore care, nutrition supply, elasticity enhancement, acne treatment, whitening and UV protection are also being launched.

As it is difficult for new single substances to be imported into Korea and large cosmetic companies are concentrating on the development of multifunctional products for whitening and UV protection, whitening materials are rapidly emerging.

Until now, the development and commercialization of cosmetic materials with skin whitening and wrinkle improvement effects have been actively carried out, and the demand for sunscreens and commercialization is increasing due to the increase in UV exposure due to the destruction of the ozone layer.

On the other hand, Angelica is a perennial herb belonging to the Umbelliferae family and is mainly distributed in Korea, China, and Japan. Angelica grown in Korea is distinguished from Chinese Angelica ( Angelica acutiloba Kitagawa ) and Japanese Angelica ( Angelica sinensis Diels ). Angelica is a representative herbal medicine in Korea that has been used for the purpose of treating diseases and promoting health as a herbal medicine with no side effects due to its warm medicinal properties, sweet taste, and non-toxic since ancient times. The medicinal ingredients of Angelica grown in Korea include decursin, decursinol, angelate, nodakenetin, nodakenin, umbelliferone, It contains β-sitosterol, α-pinene, and limonene. In particular, among the coumarin derivatives, which are the main components of Angelica, decursin and decursinol angelate are the most abundant components, and pharmacological effects such as antibacterial, antiangiogenic, and anticancer are known.

There is a demand for a cosmetic composition that is effective in antioxidant, skin whitening, and UV protection using Angelica chia.

Republic of Korea Patent Publication No. 2020-0048185 Republic of Korea Patent No. 2141575

It is an object of the present invention to provide a cosmetic composition for improving skin whitening and blocking ultraviolet rays containing an Angelica asiatica ultrasound extract as an active ingredient.

In addition, another object of the present invention is to provide a health functional food for improving skin whitening and blocking ultraviolet rays containing Angelica asiatica ultrasound extract as an active ingredient.

The cosmetic composition for improving skin whitening and blocking ultraviolet rays of the present invention for achieving the above object may contain Angelica asiatica ultrasound extract as an active ingredient.

The ultrasonic wave Angelica extract was extracted under water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, and water was preferably used as a solvent.

The ultrasonic wave Angelica extract may be processed for 5 to 60 minutes with an ultrasonicator of a frequency of 20 to 50 kHz and a power of 50 to 700 W.

The extraction temperature during the ultrasonic treatment may be 50 to 90 ℃.

The ultrasonic wave Angelica extract may be a fermented Angelica asiatica fermented extract as a strain.

The strain is Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus sakei ( Lactobacillus sakei ), Leuconostoc mesenteroides ( Leuconostoc mesenteroides ) and Lactobacillus buchneri ( Lactobacillus buchneri ) at least one selected from the group consisting of have.

In addition, the health functional food for improving skin whitening and blocking ultraviolet rays of the present invention for achieving the above other object may contain Angelica asiatica ultrasound extract as an active ingredient.

The ultrasonic wave Angelica extract may be a fermented Angelica asiatica fermented extract.

The cosmetic composition containing the Angelica asiatica ultrasonic extract of the present invention as an active ingredient is non-toxic, has an excellent antioxidant effect, and is effective in improving skin whitening and blocking UV rays.

In addition, the effectiveness is improved through the fermentation of the ultrasonic wave extract of the present invention using a probiotic strain.

The present invention relates to a cosmetic composition and a health functional food containing an Angelica oleracea extract as an active ingredient.

Hereinafter, the present invention will be described in detail.

The cosmetic composition of the present invention contains an Angelica asiatica ultrasonic extract as an active ingredient, and the Angelica asiatica ultrasonic extract is an extract obtained by ultrasonically extracting Angelica asiatica under a solvent or an extract extracted with a solvent under the ultrasonication into a strain, preferably a probiotic strain. It may be a fermented extract.

The Angelica bait is effective for symptoms of rising qi due to severe coughing, hoarseness, chilling skin symptoms, and all boils or swellings.

The ultrasonic wave Angelica extract is extracted through sonication under an extraction solvent. Specifically, Angelica asiatica and the extraction solvent are mixed in a weight ratio of 1: 5 to 25, preferably 1: 10 to 20, and 20 to 50 kHz, preferably Treated with an ultrasonicator at a frequency of 30 to 40 kHz and a power of 50 to 700 W, preferably 150 to 400 W, at 50 to 90° C., preferably 60 to 70° C. for 5 to 60 minutes, preferably 25 to 30 minutes do.

In the case of extracting Angelica basil not ultrasonically but with solvent extraction or ultra-high pressure extraction, not only a small amount of active ingredients are extracted, but also antioxidant, skin whitening and UV protection effects may be low.

When the weight ratio of the Angelica lily and the extraction solvent is out of the above range, the active ingredient of the Angelica basil may be extracted in a small amount in the extract.

In addition, when the frequency and power of the ultrasonicator are less than the lower limit, the active ingredient of Angelica can be extracted in a small amount.

In addition, when the extraction temperature and extraction time are less than the lower limit, the active ingredient of Angelica can be extracted in a small amount.

The extraction solvent for extracting the extract is water, a lower alcohol having 1 to 4 carbon atoms, ethylene glycol, ethyl ether, or a mixed solvent thereof. As the lower alcohol, 20 to 99% by volume of an aqueous solution of methanol, ethanol, butanol or propanol may be mentioned, and preferably water for excellent antioxidant, skin whitening and UV protection.

The fermented Angelica asiatica extract of the present invention is obtained by fermenting the fermented Angelica asiatica ultrasonic extract into a strain.

The Angelica fermented extract of the present invention is one that is fermented for 10 to 30 days at 25 to 50° C. at 100 to 300 rpm after inoculating the strain into the ultrasonic Angelica asiatica extract, preferably, the Angelica gorilla ultrasonic hot water extract.

As the strain, Lactobacillus plantarum ( Lactobacillus plantarum ), Lactobacillus sakei ( Lactobacillus sakei ), Leuconostoc mesenteroides ( Leuconostoc mesenteroides ) and Lactobacillus buchneri ( Lactobacillus buchneri ) at least one selected from the group consisting of and beta-When using a strain other than the strain known to produce glucosidase, antioxidant, skin whitening and UV blocking effects may be low or absent.

When the temperature, mixing speed, and time during fermentation are less than the lower limit, the active ingredient of Angelica can be extracted in a small amount, and if it exceeds the upper limit, the desired effect may not be exhibited at all due to the decomposition of the physiologically active material.

The ultrasonic wave Angelica asiatica extract and the fermented Angelica asiatica extract of the present invention may be used in health functional foods in addition to cosmetic compositions.

The term 'extract' used while referring to Angelica in the present specification includes not only the crude extract obtained by treating the extraction solvent, but also the processed product of the Angelica fermented extract or the Ultrasonic Angelica extract. For example, the Ultrasonic Angelica Root Extract or the Angelica fermented extract may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze-drying or spray-drying.

On the other hand, in the present specification, the term 'contained as an active ingredient' means including an amount sufficient to achieve the efficacy or activity of the angelica fermented extract or ultrasonic ultrasonic extract. For example, the ultrasonic wave extract of Angelica keiskei or fermented Angelica ferment extract is used at a concentration of 10 to 1500 μg/ml, preferably 100 to 1000 μg/ml. Since the angelica ultrasonic extract or the angelica fermented extract is a natural product, there is no side effect to the human body even when used in excess, so that the upper limit of the quantitative upper limit of the ultrasonic elemental angelica extract or fermented angelfish extract included in the composition of the present invention can be selected and carried out by those skilled in the art within an appropriate range.

In the cosmetic composition of the present invention, in addition to the cosmetic composition described above, other ingredients commonly formulated in cosmetics may be blended if necessary. Examples of such blending ingredients include oil and fat ingredients, moisturizers, emollients, surfactants, organic and inorganic pigments, Organic powder, UV absorber, preservative, disinfectant, antioxidant, pH adjuster, alcohol, colorant, flavoring agent, blood circulation promoter, cooling agent, limiting agent, purified water, water-soluble vitamin, fat-soluble vitamin, polymer peptide, polymer polysaccharide, sphingolipid and seaweed an extract, etc. are mentioned.

The cosmetic composition of the present invention may be prepared in the form of emulsified formulations and solubilized formulations commonly used in the art.

In addition, the ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the ingredients as an active ingredient, for example, conventional adjuvants such as stabilizers, pigments and natural fragrances; It may further include a carrier.

Products to which the composition of the present invention can be added include, for example, mist, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, sunscreen cream , moisture cream, hand cream, foundation, essence, nourishing essence, mask pack, press powder, loose powder, eye shadow, etc., and soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane , butane or propellants such as dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

In addition, the present invention provides a health functional food composition comprising an angelica fermented extract or an ultrasonic angelfish extract as an active ingredient.

Health functional food is food prepared by adding or encapsulating, powdering, or suspension to food ingredients such as beverages, teas, spices, gums, and confectionery obtained by adding Angelica asiatica ultrasonic extract or fermented Angelica asiatica extract. It means to bring an effect, but unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long period of time by using food as a raw material. The health functional food of the present invention obtained in this way is very useful because it can be ingested on a daily basis. The added amount of Angelica spp. ultrasonic extract or Angelica fermented extract in such health functional food varies depending on the type of target health functional food and cannot be defined uniformly, but it can be added within a range that does not impair the original taste of the food. It is usually in the range of 0.01 to 50% by weight, preferably 0.1 to 20% by weight relative to food. In addition, in the case of a health functional food in the form of pills, granules, tablets or capsules, it is usually added in an amount of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a pill, tablet, capsule or beverage.

Hereinafter, preferred examples are presented to help the understanding of the present invention, but the following examples are merely illustrative of the present invention, and it will be apparent to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It goes without saying that such variations and modifications fall within the scope of the appended claims.

Example 1. Angelica Ultrasonic Extract

Angelica and water were mixed in a weight ratio of 1:20, put into an ultrasonicator (JAC Ultrasinic, Hwaseng, Korea), and then extracted for 30 minutes using ultrasonic waves (40 kHz, 250 W) at 60 ° C. Then, the extract was extracted at 1000 rpm for 5 minutes. During centrifugation, the supernatant was recovered to obtain an Angelica sonicum extract.

Example 2. Angelica fermented extract

culture medium

Leukonostok mecenteroides was inoculated with 1% MRS broth and cultured at 37° C. for 24 hours to be used as a culture medium.

fermented extract

After cooling the ultrasonic wave Angelica extract prepared in Example 1 at room temperature, the ultrasonic hot water extract and Leukonostok mecenteroides were mixed in a weight ratio of 1: 2 to adjust the pH to 6.95 to 7.05, and then to 100 parts by weight of the mixture. Add 1 part by weight of sugar, ferment at 37°C for 48 hours, crush using a homogenizer (HG-15A, DAIHAN, Korea), adjust pH to 4.78±0.05, ferment at 45°C for 48 hours, and centrifuge to recover the supernatant By doing so, an angelica ferment extract was obtained.

Comparative Example 1. Angelica common extract

Angelica quai and water were mixed in a weight ratio of 1:20, extracted at 100° C. for 60 minutes, and the extract was centrifuged at 1000 rpm for 5 minutes to recover the supernatant to obtain an Angelica basil extract.

Comparative Example 2. Angelica ultra-high pressure extract

Angelica and water were mixed in a weight ratio of 1:20, put into an ultra-high pressure extractor (autoclave), and extracted for 30 minutes under a pressure of 1000 atm and a temperature of 300 ° C. Then, the extract was centrifuged at 1000 rpm for 5 minutes to obtain a supernatant. By recovering, an ultra-high pressure extract of Angelica keis was obtained.

Comparative Example 3. Angelica fermented extract_Comparative Example 1

It was carried out in the same manner as in Example 2, except that the angelica fermented extract of Comparative Example 1 was used instead of the ultrasonic extract of Angelica keisi in Example 1.

Comparative Example 4. Angelica fermented extract_Comparative Example 2

It was carried out in the same manner as in Example 2, except that the ultra-high pressure Angelica asiatica extract of Comparative Example 2 was used instead of the Angelica basil ultrasonic extract of Example 1 to obtain a fermented Angelica basil extract.

<Test Example>

Test Example 1. Measurement of total polyphenol content, total flavonoid content and DPPH scavenging ability

1-1. Total polyphenol content (mg GAE/g DW): Measured by modifying the Folin-Denis method, folin-ciocalteu's phenol solution (Folin &Ciocalteu'sphenol; Sigma-Aldrich, USA) was added to the sample and reduced by a polyphenol compound The molybdenum blue color reaction generated by the process was based on the principle. After adding 0.2 N FC solution to 0.14 ml of the sample and leaving it for 10 minutes, 0.56 ml of 7.5% Na ₂ CO ₃ was added and reacted for 1 hour, and the absorbance value was measured at 756 nm. Gallic acid (Sigma-Aldrich, USA) was used as a standard material, and the unit was expressed as mg gallic acid equivalent (GAE)/g dry weight (DW) compared with the prepared gallic acid calibration curve.

1-2. Total flavonoid content (mg QE/g DM): A modified method of Zhishen et al. was used. TFC concentration was measured by measuring absorbance based on the principle that yellow color develops when alkali is applied to flavonoids. To 0.5 mL of each sample, 2.5 mL of distilled water and 1.5 mL of 99.5% (v/v) ethanol were added, then 0.1 mL of 1 M potassium acetate and 0.1 mL of 10% aluminum chloride were added, stirred, and left at room temperature for 30 minutes. Absorbance was measured at 415 nm, and a calibration curve was obtained by diluting quercetin (Sigma-Aldrich, Minneapolis, USA) to 25, 50, 100, 200 ug/mL to determine the flavonoid content in mg quercetin equivalent (QE)/g dry matter (DM) indicated as

1-3. DPPH scavenging ability (%): The electron donating ability was measured by modifying the method of Blois, and DPPH (2,2-Diphenyl-1-picrylhydrazyl, Sigma -Aldrich) The reducing power of the sample was measured through free radical scavenging activity. After adding 1.25 ml of DPPH solution to 0.25 ml of sample and reacting in the dark for 20 minutes, absorbance was measured at 517 nm. Based on the absorbance of the control group to which the sample was not added, the DPPH radical scavenging activity was obtained according to [Equation 1] below. indicated as

[Equation 1]

DPPH radical scavenging activity (%)={1-(Abs(test)-Abs(color))/Abs(control)}X100

division Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 total polyphenols
(mg GAE/g DW) 9.226 27.678 2.195 2.105 5.227 7.436 total flavonoids
(mg QE/g DW) 1.133 0.557 0.299 0.272 0.367 0.315 DPPH scavenging ability (%) 18.336 26.909 10.198 11.450 12.088 16.679

As shown in Table 1 above, it was confirmed that the ultrasonic wave Angelica extract prepared according to Example 1 of the present invention had higher total polyphenol content and total flavonoid content than Comparative Examples 1 and 3, and excellent DPPH scavenging ability.

In addition, it was confirmed that the fermented Angelica asiatica prepared according to Example 2 of the present invention had higher total polyphenol content and total flavonoid content than Comparative Examples 2 and 4 and excellent DPPH scavenging ability.

In particular, it was confirmed that Comparative Example 3, in which the extract of Comparative Example 1 was fermented, and Comparative Example 4, in which the extract of Comparative Example 2 was fermented, did not rapidly improve the total polyphenol content and DPPH scavenging ability during fermentation, unlike Example 2 of the present invention. did

Test Example 2. Tyrosinase (Tyrosinase) activity inhibition

Measurement of inhibition of tyrosinase activity: The inhibitory activity of tyrosinase was tested by modifying Flurkey's method. A buffer was prepared by dissolving 0.8039 g of sodium phosphate monobasic anhydrous and 0.9511 g of sodium phosphate dibasic anhydrous in 100 mL of distilled water, respectively, and adjusting the pH to 6.8. As the prepared buffer, the substrate 10 mM 3,4-dihydroxy phenylanin (I-dopa) (20 mg/mL) and the enzyme 1250 unit tyrosinase were prepared, and 1250 unit tyrosinase was diluted 10-fold in distilled water and used. As a positive control, kojic acid (2 mg/mL) was prepared. In a 2 mL microtube, 0.4 g of buffer, 0.2 g of substrate, 0.2 g of sample, and 0.2 g of enzyme were sequentially added and stirred, followed by reaction at 25° C. for 30 minutes. After the reaction, absorbance was measured at 475 nm.

[Equation 2]

Tyrosinase inhibition rate (inhibitory activity, %) = [1-sample OD / control OD] * 100

division Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Tyrosinase inhibition rate (%) 16.667 47.650 5.602 7.115 8.688 15.195

As shown in Table 2 above, it was confirmed that the ultrasound extract of Angelica asiatica prepared according to Example 1 of the present invention had a higher tyrosinase inhibition rate than Comparative Examples 1 and 3.

In addition, it was confirmed that the fermented Angelica basil extract prepared according to Example 2 of the present invention also had a higher tyrosinase inhibition rate than Comparative Examples 2 and 4.

In particular, it was confirmed that Comparative Example 3, in which the extract of Comparative Example 1 was fermented, and Comparative Example 4, in which the extract of Comparative Example 2 was fermented, did not rapidly improve the tyrosinase inhibition rate during fermentation, unlike Example 2 of the present invention.

Test Example 3. UV protection

In order to measure the UV blocking rate of the Angelica extracts prepared according to Examples and Comparative Examples, the blocking rate was measured in the UV A (320-400 nm) and UV B (290-320 nm) wavelength ranges using a UV/Vis spectrophotometer. The absorbance was measured by comparing it with the UV blocking ability of distilled water and tannin 10 mg/mL, a representative polyphenol.

division Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 UV-A blocking rate (%)
tannin contrast 11.3 25.0 5.6 9.4 7.8 10.2 UV-B blocking rate (%)
tannin contrast 32.3 58.0 18.5 22.4 20.6 23.7

As shown in Table 3 above, the Angelica asiatica ultrasound extract prepared according to Example 1 of the present invention has a higher UV blocking rate compared to Comparative Examples 1 and 3, so it is effective in blocking UV rays touching the skin.

In addition, the fermented Angelica ferment extract prepared according to Example 2 of the present invention also has a higher UV blocking rate compared to Comparative Examples 2 and 4, so it is effective in blocking UV rays coming into contact with the skin.

That is, since the extracts of Examples 1 and 2 of the present invention block ultraviolet rays in the cosmetic composition applied to the skin, there is almost no ultraviolet rays directly touching the skin.

Test Example 4. Measurement of cell viability by MTT assay

In order to evaluate the effect of the extracts of Examples and Comparative Examples on cytotoxicity, MTT assay was used. MTT assay may be referred to as a standard colorimetric assay as a laboratory test method for measuring cell viability. MTT assay, a technique that can accurately measure cell proliferation and living cells, is one of the essential techniques in the field of life sciences, especially in tumor biology. In order to search for efficacy for the development of new anticancer drugs or to examine the sensitivity of previously developed anticancer drugs, the process of objectively proving that the drug inhibits the growth of tumor cells in vitro must be preceded before application to the living body, such as animal experiments. .

B16F0 cell line (melanoma cells derived from mice) diluted to 2X10 ³ cells/ml was added to each plate of a 96-well plate, and the strain was incubated at 37°C, 5% CO2 incubator for 24 hours. After culturing for 24 hours, the concentration of the composition of the present invention was diluted to 4 mg/ml and added to each well, and cisplatin was diluted in the same manner and added to the wells.

Then, it was cultured again at 37°C, 5% CO2 incubator for 24 hours, and after adding 50 μl of 2 mg/ml MTT reagent, it was left in an incubator at 37°C for 4 hours.

The supernatant was removed using a centrifuge, and 200 μl of DMSO was added to each well to dissolve the MTT stained precipitate, and then the OD540 value was measured at a wavelength of 540 nm with an ELISA reader. The 50% inhibitory concentration (IC50) is the concentration of the drug that results in a 50% survival rate.

division Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Cell viability (%, IC50=4mg/ml) 3.4 48.1 3.1 12.9 3.5 20.4

As shown in Table 4 above, it was confirmed that the extracts prepared according to Examples 1 and 2 and the extracts of Comparative Examples 1 to 4 of the present invention suppressed excellent cancer cell proliferation.

In particular, Example 2 suppressed the proliferation of cancer cells by 48.1%, so it was confirmed that the ability to inhibit the proliferation of cancer cells was superior to that of the other groups.

Hereinafter, formulation examples of the composition containing the powder of the present invention will be described, but the present invention is not intended to limit the present invention, but merely to describe it in detail.

Preparation Example 1. Preparation of granules

1,000 mg of fermented product powder obtained in Example 2

appropriate amount of vitamin mixture

70 μg vitamin A acetate

Vitamin E 1.0 mg

Vitamin B1 0.13 mg

Vitamin B2 0.15 mg

0.5 mg of vitamin B6

0.2 μg of vitamin B12

Vitamin C 10 mg

Biotin 10 μg

Nicotinamide 1.7 mg

50 μg of folic acid

Calcium pantothenate 0.5 mg

Mineral mixture appropriate amount

ferrous sulfate 1.75 mg

Zinc Oxide 0.82 mg

Magnesium carbonate 25.3 mg

potassium phosphate monobasic 15 mg

Dibasic calcium phosphate 55 mg

Potassium citrate 90 mg

100 mg of calcium carbonate

Magnesium chloride 24.8 mg

The composition ratio of the vitamin and mineral mixture is relatively suitable for granules in a preferred embodiment, but the mixing ratio may be arbitrarily modified. It can be prepared and used in the preparation of a health functional food composition according to a conventional method.

Preparation Example 2. Preparation of functional beverage

1,000 mg of fermented product powder obtained in Example 2

1,000 mg citric acid

100 g of oligosaccharides

2 g of plum concentrate

1 g taurine

Add purified water to total 900 mL

After mixing the above ingredients according to the usual health drink manufacturing method, after stirring and heating at 85 ° C for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container, sealed and sterilized, then refrigerated. It is used to prepare the functional beverage composition of the present invention.

Although the composition ratio is prepared by mixing ingredients suitable for relatively favorite beverages in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and ethnic preferences such as demand class, demanding country, and use.

A preparation example of a cosmetic composition suitable for applying the present invention will be presented.

Preparation example 3: lotion

Examples of the preparation of the lotion among the cosmetics including the fermented product of Example 2 are shown in Table 5 below.

ingredient content (wt%) Fermented product of Example 2 5.0 glycerin 6.0 1,3-butylene glycol 3.0 PG 1500 1.0 allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium Hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Preparation Example 4: Lotion

Preparation examples of the lotion in the cosmetic containing the fermented product of Example 2 are shown in Table 6 below.

ingredient content (wt%) Fermented product of Example 2 3.0 propylene glycol 6.0 glycerin 4.0 triethanolamine 1.2 tocopheryl acetate 3.0 liquid paraffin 5.0 squalane 3.0 Macadamina Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 1.0 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Preparation 5: Nourishing Cream

Preparation examples of the nutritional cream in the cosmetic comprising the fermented product of Example 2 are shown in Table 7 below.

ingredient content (wt%) Fermented product of Example 2 1.0 lipophilic monostearate glycerin 1.5 cetearyl alcohol 1.5 stearic acid 1.0 Polysorbate 60 1.5 Sorbitan Stearate 0.6 Isostearylisostearate 5.0 squalane 5.0 mineral oil 35.0 dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 triethanolamine 0.7 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Preparation Example 6: Essence

Preparation examples of the essence in the cosmetic including the fermented product of Example 2 are shown in Table 8 below.

ingredient content (wt%) Fermented product of Example 2 1.5 glycerin 10.0 betaine 5.0 PEG 1500 2.0 allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl Cellulose 0.1 Sodium Hyaluronate 8.0 Carboxyvinyl Polymer 0.2 triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeces-16 0.4 ethanol 6.0 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Preparation Example 7: Emulsion for mask pack

Preparation examples of the emulsion for a mask pack among the cosmetics containing the fermented product of Example 2 are shown in Table 9 below.

ingredient content (wt%) Fermented product of Example 2 1.0 polyvinyl alcohol 15.0 Cellulose Gum 0.15 glycerin 3.0 PEG 1500 2.0 cyclodextrin 0.15 DL-Panthenol 0.4 allantoin 0.1 Monoammonium glycyrrhizinate 0.3 nicotinamide 0.5 ethanol 6.0 PEG 40 hydrogenated castor oil 0.3 preservatives, fragrances, colors a very small amount Distilled water remaining amount Sum 100

Claims (9)

Containing Angelica fermented extract obtained by fermenting Angelica spp. ultrasonic extract with a strain as an active ingredient,
The strain is Leuconostoc mesenteroides ( Leuconostoc mesenteroides ) Skin whitening improvement and UV protection cosmetic composition, characterized in that. [Claim 2] The cosmetic composition for improving skin whitening and blocking UV rays according to claim 1, wherein the ultrasonic wave Angelica extract is extracted under water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The cosmetic composition for improving skin whitening and blocking ultraviolet rays according to claim 2, wherein the ultrasonic wave Angelica extract is an extract extracted with water. [Claim 2] The cosmetic composition for improving skin whitening and blocking UV rays according to claim 1, wherein the ultrasonic wave extract of Angelica basilica is treated for 5 to 60 minutes with an ultrasonic wave having a frequency of 20 to 50 kHz and a power of 50 to 700 W. [Claim 5] The cosmetic composition for improving skin whitening and blocking UV rays according to claim 4, wherein the extraction temperature during the ultrasonic treatment is 50 to 90 °C. delete delete Containing Angelica fermented extract obtained by fermenting Angelica spp. ultrasonic extract with a strain as an active ingredient,
The strain is Leuconostoc mesenteroides ( Leuconostoc mesenteroides ) Health functional food for skin whitening improvement and UV protection, characterized in that it is. delete