KR102154139B1 - Composition comprising fermentation of sap of painted maple, cacao nibs extract and granat extract - Google Patents

Abstract

The present invention relates to a cosmetic composition and/or a food composition, comprising a fermented liquor of acer mono sap, a cacao nibs extract, and a Punica granatum Linne. The composition may be used as a composition for antioxidant and alleviating inflammation.

Description

A composition comprising fermented broth, cacao nibs extract, and pomegranate peel extract from sap of the sap of Goro S. {COMPOSITION COMPRISING FERMENTATION OF SAP OF PAINTED MAPLE, CACAO NIBS EXTRACT AND GRANAT EXTRACT}

The present invention relates to a cosmetic composition and/or a food composition comprising a fermented broth, cacao nibs extract, and pomegranate peel extract of the sap of Goro-so-tree. The composition may be used as a composition for improving antioxidant and inflammation.

Skin aging is an intrinsic (chronological aging) phenomenon that occurs naturally due to the decrease in physiological functions of the living body as age increases, and photoaging in which the appearance or function of the skin changes as the skin is repeatedly exposed to light. have. However, recently, research has been reported that as the earth's temperature rises, the increase in skin temperature due to the heat of light is also an important cause of skin aging. It is proved that as the skin is exposed to heat and the skin temperature rises, collagen and elastin, which are major components of the skin, decrease, and the decomposition of the component protein is accelerated to promote skin aging, such as wrinkles. However, up to now, sunscreens cannot solve the rise in skin temperature, which is an important cause of skin aging.

On the other hand, as various studies reveal the process of controlling skin temperature, attempts to lower the skin temperature are underway. According to a recent study, it has been found that transient receptor potential channels (TRP channels) play an important role in the process of temperature signaling, and phosphatidylinositol diphosphate and diacylglycerol (phosphatidylinositol diphosphate) and diacylglycerol (via G-protein) play an important role. diacylglycerol), protein kinase C.

The TRP channel, a temporary sensory receptor, is a non-specific cation channel that is widely expressed in the skin. It is mainly responsible for the influx of calcium ions into cells, and functions for various senses such as light stimulation, mechanical stimulation, temperature stimulation, chemical stimulation, and osmotic pressure change. This is known. Among them, TRPV1 (Transient Receptor Potential Vanilloid1) is a thermal receptor and a pungent receptor that functions as a sensor for harmful stimuli such as ultraviolet rays. That is, since TRPV1 can be a target for preventing skin photoaging caused by exposure to ultraviolet rays, etc., many studies are being conducted as a skin temperature control mechanism to prevent and prevent skin aging.

Therefore, as described above, in order to solve the increase in skin temperature, which is an important cause of skin aging, it is necessary to discover natural materials that do not have toxicity and side effects, and that can control skin temperature.

Korean Patent Publication No. 10-2018-0083835

It is an object of the present invention to provide a cosmetic composition comprising a fermentation broth, cacao nibs extract, and pomegranate peel extract of the sap of Goro-so-so.

Another object of the present invention is to provide a food composition comprising a fermented broth, cacao nibs extract, and pomegranate peel extract of the sap of Goro-sero.

One aspect of the present invention for achieving the above object relates to a cosmetic composition comprising a fermented broth, cacao nibs extract, and pomegranate peel extract of the sap of Goro-sob.

In the present invention, the “Sap of Goro-seonsa” refers to a liquid that flows in about 10 kinds of maple trees that grow wildly at an altitude of 500 to 1000 m above sea level. The sap of the Gorosevils tree is known to be effective in postpartum diseases, neuralgia, gastrointestinal diseases, and high blood pressure due to its high mineral content, and is also known to be effective in preventing osteoporosis. In particular, it is effective in removing inflammation and relieving pain.

The fermentation broth of the sap of Goro-sobum refers to a solution obtained by inoculating the strain into the sap of Goro-soburus. Specifically, the fermentation broth of the sap of Goro-so-namu may be fermented by inoculating yeast.

In the present invention, "fermentation" refers to a process of decomposing organic matter using enzymes possessed by the fermentation strain itself. It is known that raw materials that have undergone a fermentation process through a fermentation strain have an effect of at least two to tens of times greater than before fermentation. Metabolites that have undergone fermentation contain various amino acids, organic acids, and antioxidants that are good for the skin to promote skin metabolism and smooth the skin texture. In addition, through the fermentation process, the particles become smaller and toxicities such as heavy metals are decomposed, so that the absorption rate is good, and skin troubles and allergic side effects are alleviated. Furthermore, by fermentation using beneficial bacteria such as lactic acid bacteria and yeast, it is possible to improve the efficacy of promoting and/or promoting beneficial bacteria in the ingredients.

More specifically, the yeast is Saccharomyces cerevisiae , Saccharomyces byanus , Saccharomyces uvarum , Saccharomyces pastorianus , and Saccharomyces pastorianus . It may be any one selected from the group consisting of Mrs. paradoxus ( Saccharomyces paradoxus ).

In the present invention, “cacao nibs” means that cacao beans, the seeds of cacao trees, are fermented, dried, and then crushed to an appropriate size through a roasting process. Cacao Nibs is known to be excellent in vasodilation and health promotion, diabetes improvement and diet as it contains procyanine. It is especially rich in catechins, which removes free radicals in the body and prevents cell damage, so it is effective in preventing aging. have.

In the present invention, " Punica granatum Linne " is a dried fruit of a pomegranate tree, a deciduous small arboreous tree belonging to the pomegranate family, and is also referred to as sanryu blood, mountain pomegranate skin, anseokryu mountain silgak, and pomegranate. Pomegranate contains a lot of vitamins and is known as a good fruit for women because it contains a lot of plant estrogen, a component similar to female hormones. Furthermore, it also contains anthocyanin and lycopene, which is excellent for vascular health. In particular, pomegranate peel, the peel of pomegranate fruit, contains a large amount of antioxidants such as vitamin C, polyphenol, elazitanine, gallotanine, funicalazine, ellagic acid, and anthocyanin.

The "extract" of the present invention is an extract obtained by extraction treatment of cacao nibs and/or pomegranate blood, a dilution or concentrate of the extracts, a dried product obtained by drying the extract, a preparation or purified product of the extract, or a mixture thereof, etc. , The extract itself and all formulations that can be formed using the extract are included.

The extract of the present invention may be extracted from natural, hybrid or variety plants of the cacao nibs and/or pomegranate peel, and may be extracted from plant tissue cultures.

The method of extracting the extract of the present invention is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, which may be performed alone or in combination of two or more methods.

The type of the extraction solvent used in the present invention is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water, C ₁ to C ₄ anhydrous or lower alcohol, a mixed solvent of water and lower alcohol, acetone, 1,3-butylene glycol, ethyl acetate, selected from the group consisting of chloroform It can be used alone or in combination of two or more.

More specifically, purified water may be used as the extraction solvent in the present invention. An extract may be prepared by hot water extraction of cacao nibs and/or pomegranate blood using the solvent at least once, and a dried extract obtained by distilling the solvent extract under reduced pressure and then freeze drying or spray drying may be prepared. In a specific embodiment of the present invention, hot water extraction was performed to obtain cacao nibs and/or pomegranate peel extract (Example 1).

In the present invention, the extract obtained by heat extraction or cold needle extraction is filtered to remove suspended solid particles, for example, nylon, etc. to filter the particles, or after filtration using a freeze filtration method, etc., and then used as such, or freeze-dried, hot-air drying, It can be used after drying by spray drying or the like.

In the present invention, the inventors prepared a mixed composition containing the fermented broth of the sap of the gorse tree sap, cacao nibs extract, and pomegranate peel extract in a constant ratio, and it was found through specific experiments that the composition is excellent in efficacy in improving skin antioxidants and skin inflammation. . Accordingly, the composition is intended to be used in a cosmetic composition.

In an embodiment of the present invention, in order to confirm the skin stability of the cosmetic composition comprising the fermentation broth of the sap of the Goro-sera, cacao nibs extract, and pomegranate peel extract, as a result of evaluating the cytotoxicity of skin keratinocytes, It was confirmed that the stability was high (Table 1).

In the present invention, the cosmetic composition comprising the fermentation broth of the sap of the gorse tree sap, the cacao nibs extract and the pomegranate peel extract may be used for antioxidant purposes or for improving inflammation.

In the present invention, “antioxidation” refers to an action of inhibiting oxidation, and in the human body, an oxidation promoting substance and an oxidation inhibitor are in balance, but due to various factors, the balance is lost and the direction of promoting oxidation When inclined toward, oxidative stress is induced in a living body, leading to cell damage and pathological diseases. Reactive oxygen species (ROS), which are the direct cause of oxidative stress, are chemically unstable and highly reactive, so they can easily react with various biomaterials such as DNA, proteins, lipids, and carbohydrates. Attacks cause irreversible damage to cells and tissues, or cause mutations, cytotoxicity and cancer, and are also a direct cause of aging. By removing or reducing these reactive oxygen species, antioxidant effects are obtained, preventing aging and maintaining health.

In one embodiment of the present invention, the antioxidant effect was confirmed by measuring the free radical scavenging activity. In particular, it was confirmed that the antioxidant effect of the composition comprising all of the fermented broth, cacao nibs extract, and pomegranate peel extract of the present invention compared to the composition of one or two extract combinations (Tables 2 and 3).

In the present invention, "improving inflammation" refers to suppressing inflammation, and the inflammation is one of the defense responses of living tissues against certain stimuli, and complex lesions that cause three types of tissue deterioration, circulatory disorders and effusion, and tissue proliferation Say. Inflammatory reaction is a mechanism to regenerate damage caused by physical action, harmful substances, chemical stimulation, bacterial infection, etc. as part of the defense response of living tissue against external stimuli. The persistent inflammatory reaction rather causes damage to the mucous membrane and promotes damage, inflammation and skin troubles in the skin tissue.

Macrophages play a very important role in the immune response and regulate a variety of inflammatory mediators, including nitric oxide, prostaglandins, and inflammatory cytokines, an immune protein. Among them, nitric oxide plays an important role in killing bacteria or removing tumors, but excessive nitric oxide production by pathogenesis causes inflammation, and such inflammation causes skin trouble.

That is, when the production of nitric oxide by macrophages is suppressed, skin inflammation can be prevented, alleviated, improved, and treated, and skin problems caused by inflammatory reactions can be prevented, alleviated, improved, and treated.

In one embodiment of the present invention, it was confirmed that the production of nitrogen monoxide was significantly reduced when the fermentation broth, cacao nibs extract, and pomegranate peel extract were treated (Table 4), and as a result of formulating and applying the effect of improving dermatitis as an essence, It was confirmed that the effect of improving inflammation persisted (Table 6).

This suggests that the composition containing the fermented broth of the sap of Goro-so-ji, cacao nibs extract, and pomegranate peel extract is excellent in improving inflammation.

The cosmetic composition of the present invention is a solution, external ointment, cream, foam, nutrient lotion, flexible lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil, suspension, emulsion It can be prepared in a formulation selected from the group consisting of liquid, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, and spray, but is limited thereto. no.

The cosmetic composition of the present invention may additionally include one or more cosmetically acceptable carriers blended in general skin cosmetics, and as common ingredients, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners , A chelating agent, a colorant, a preservative, a fragrance, and the like may be appropriately blended, but are not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohard It may include a propellant such as locarbon, propane/butane or dimethyl ether, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, and the like may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used, but are not limited thereto. These may be used alone or in combination of two or more.

In addition, the composition of the present invention may be used as a transdermal administration method such as direct application or spraying on the skin, and the administration route of the composition of the present invention may be administered through any general route as long as it can reach the target tissue.

The amount of use of the composition of the present invention may be appropriately adjusted according to individual differences or formulations such as age and degree of lesions, and may be used from one week to several months by applying one or several times a day to the skin.

Another aspect of the present invention relates to a food composition comprising a fermented broth, cacao nibs extract, and pomegranate peel extract of the sap of Goro-so-so.

The description of the "fermented broth of the sap of the Goro-sabi", "cacao nibs extract" and "pomegranate peel extract" is the same as described above.

Specifically, the fermentation broth of the sap of Goro-so-namu may be fermented by inoculating yeast. More specifically, the yeast is Saccharomyces cerevisiae , Saccharomyces byanus , Saccharomyces uvarum , Saccharomyces pastorianus , and Saccharomyces pastorianus . It may be any one or more selected from the group consisting of Mrs. paradoxus ( Saccharomyces paradoxus ).

In addition, specifically, the food composition may be a food for improving antioxidant or skin inflammation.

The “antioxidation” and “improving inflammation” are the same as already described above.

Foods to which the fermentation broth, cacao nibs extract and pomegranate peel extract of the present invention can be added include meat, bread, sausage, chocolate, snacks, candy, confectionery, ramen, pizza, other noodles, gum, ice cream There are dairy products, various soups, beverages, tea, vitamin complexes, and health functional supplements.

The type of food may specifically be a health functional food. The health functional foods include various nutrients, vitamins, minerals (electrolytes), synthetic flavors and flavoring agents such as natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, organic acids, protective colloidal growth It may contain an agent, a pH adjuster, a stabilizer, a preservative, glycerin, alcohol, a carbonation agent used in carbonated beverages, and the like. These components may be used alone or in combination, and the proportion of these additives is generally selected from 0.001 to 50 parts by weight per total weight of the composition.

The health functional food is a food that emphasizes the biological regulation function of food, and is a food that has added value to act and express it for a specific purpose by using physical, biochemical, and bioengineering methods. Ingredients of these health functional foods are designed and processed to fully exert the body's control functions related to body defense, regulation of body rhythm, prevention and recovery of diseases to the living body, and food additives, sweeteners, or functional foods acceptable as foods. It may contain raw materials.

The health functional food may be any one formulation selected from the group consisting of tablets, granules, powders, capsules, liquid solutions, and pills, but is not limited thereto. Specifically, the health functional food in the form of a tablet is a composition comprising a fermented broth, cacao nibs extract and pomegranate peel extract, a mixture of excipients, binders, disintegrants and other additives in a conventional manner, and then a lubricant, etc. It can be prepared by compression molding or directly compression molding the mixture. In addition, the health functional food in the form of a tablet may contain a bitter agent or the like as necessary, and may be coated with a suitable coating agent if necessary.

Among the capsule-type health functional foods, the hard capsule is a mixture of additives, such as a composition and excipients, or a granular product or granular material coated with a composition comprising a fermented sap of Bronchia sap, cacao nibs extract and pomegranate peel extract in a conventional hard capsule. Can be prepared by filling. Soft capsules can be prepared by filling a mixture with additives such as a composition and excipients, including fermentation broth, cacao nibs extract, and pomegranate peel extract, in a capsule base such as gelatin. The soft capsules may contain plasticizers such as glycerin or sorbitol, colorants, preservatives, and the like, if necessary.

The ring-shaped health functional food may be prepared by molding a mixture of a fermented broth of Goro-so-so tree sap, a composition comprising a cacao nibs extract and a pomegranate extract, an excipient, a binder, a disintegrant, etc. It is also possible to coat the coat with other suitable coats, or with starch, talc, or a suitable material.

The health functional food in the form of granules can be prepared in a granular form by a suitable method, including a composition comprising a composition comprising a fermented broth of a sap of Goro-sabi, a cacao nibs extract and a pomegranate peel extract, an excipient, a binder, a disintegrant, etc. Depending on, it may contain a flavoring agent, a bitter taste, and the like.

The definition of terms for the excipients, binders, disintegrants, lubricants, bittering agents, flavoring agents, and the like are known in the art, and may include the same or similar functions such as functions.

In addition, the type of food may be a food additive, and the food additive refers to a substance used by a method such as adding, mixing, infiltrating or adding to food for manufacturing, processing, or preserving food. The food additives include natural products and synthetic products, and can be classified according to functions and uses. Currently, 370 kinds of chemical synthetic products and 50 kinds of natural additives are approved as food additives in Korea. Depending on the purpose, preservatives, fungicides, antioxidants, coloring agents, coloring agents, bleaching agents, seasonings, sweeteners, flavoring agents, expanding agents, strengthening agents, and improving agents , Emulsifiers, thickeners and stabilizers, coating agents, gum base agents, defoaming agents, solvents, releasing agents, insect repellents, quality improvers and other additives for food manufacturing.

The form of the food additive may include a powder, granule, tablet, capsule or liquid form, and specifically may be a capsule form, but is not limited to the form.

When used as a food composition containing the fermentation broth, cacao nibs extract and pomegranate peel extract of the present invention, the fermentation broth, cacao nibs extract, and pomegranate bark extract of the sap of Goro-sobium are added as they are or used with other foods or food ingredients. And, it can be appropriately used according to a conventional method. The mixing amount of the fermented broth, cacao nibs extract, and pomegranate peel extract of the sap of Goro-so-shi can be suitably determined according to the purpose of use (prevention, health or improvement, therapeutic treatment).

The cosmetic composition comprising the fermented broth, cacao nibs extract, and pomegranate peel extract of the present invention has excellent antioxidant and inflammation improvement effects, and is highly utilized as a cosmetic composition and/or food composition for antioxidant or inflammation improvement.

The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be deduced from the configuration of the invention described in the detailed description or claims of the present invention.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only illustrative of the present invention, and the present invention is not limited by the following examples.

Example 1. Saccharomyces cerevisiae ( Saccharomyces cerevisiae A composition comprising fermented broth, cacao nibs extract, and pomegranate peel extract using the sap

After inflicting some wounds on the goryeosae trees that grow naturally in the Samsan Yugok, a clean container was attached to collect the naturally flowing sap to obtain pure Goro’s tree sap. Afterwards, yeast ( Saccharomyces cerevisiae ) bacteria were added to the collected sap of Goro-sae in an amount of 2 to 5 parts by weight based on 100 parts by weight of sap of Go-ro-sob and fermented at 40°C for 48 hours. Then, 30% of butylene glycol was added to the fermented raw material, extracted for 7 days at room temperature, filtered through a 400 mesh filter cloth, and filtered through a 0.45 μm filter to prepare a fermentation broth.

The cacao nibs and pomegranate skin were washed with purified water, dried, and coarsely pulverized, followed by high-pressure hot water extraction at 121°C for 15 minutes with 20 times the extraction solvent (purified water). The extracted cacao nibs and pomegranate peel extracts were centrifuged to recover the extracted supernatant, and the extract was filtered through a filter having a size of 0.2 μm to prepare cacao nibs and pomegranate peel extracts, respectively. The extract-filtered cacao nibs and pomegranate skin extract were lyophilized and used.

Thereafter, the fermented broth, cacao nibs extract, and pomegranate peel extract of the sap of Goro-so-ji were mixed in a ratio of 1:1 to 1 and used.

Example 2. Saccharomyces bianus ( Saccharomyces byanus A composition comprising fermented broth, cacao nibs extract, and pomegranate peel extract using the sap

It was prepared in the same manner as in Example 1. However, yeast Saccharomyces cerevisiae instead A fermentation broth was prepared using Saccharomyces bianus.

Example 3. Saccharomyces Yubarum ( Saccharomyces uvarum A composition comprising fermented broth, cacao nibs extract, and pomegranate peel extract using the sap

It was prepared in the same manner as in Example 1. However, yeast Saccharomyces cerevisiae instead A fermentation broth was prepared using Saccharomyces yubarum.

Example 4. Saccharomyces pastorian ( Saccharomyces pastorianus A composition comprising fermented broth, cacao nibs extract, and pomegranate peel extract using the sap

It was prepared in the same manner as in Example 1. However, yeast Saccharomyces cerevisiae instead A fermentation broth was prepared using Saccharomyces pastorianus.

Example 5. Saccharomyces paradoxus ( Saccharomyces paradoxus A composition comprising fermented broth, cacao nibs extract, and pomegranate peel extract using the sap

It was prepared in the same manner as in Example 1. However, yeast Saccharomyces cerevisiae instead A fermentation broth was prepared using Saccharomyces paradoxus.

Comparative Example 1. Composition containing fermentation broth of sap of Goro-sabi

After inflicting some wounds on the goryeosae trees that grow naturally in the Samsan Yugok, a clean container was attached to collect the naturally flowing sap to obtain pure Goro’s tree sap. Afterwards, yeast ( Saccharomyces cerevisiae ) bacteria were added to the collected sap of Goro-sae in an amount of 2 to 5 parts by weight based on 100 parts by weight of sap of Go-ro-sob and fermented at 40°C for 48 hours. Then, 30% of butylene glycol was added to the fermented raw material, extracted for 7 days at room temperature, filtered through a 400 mesh filter cloth, and filtered through a 0.45 μm filter to prepare a fermentation broth.

Comparative Example 2. Composition comprising fermentation broth of sap of Goro serrata and cacao nibs extract

After inflicting some wounds on the goryeosae trees that grow naturally in the Samsan Yugok, a clean container was attached to collect the naturally flowing sap to obtain pure Goro’s tree sap. Afterwards, yeast ( Saccharomyces cerevisiae ) bacteria were added to the collected sap of Goro-sae in an amount of 2 to 5 parts by weight based on 100 parts by weight of sap of Go-ro-sob and fermented at 40°C for 48 hours. Then, 30% of butylene glycol was added to the fermented raw material, extracted for 7 days at room temperature, filtered through a 400 mesh filter cloth, and filtered through a 0.45 μm filter to prepare a fermentation broth.

Each cacao nibs was washed with purified water, dried, and coarsely pulverized, followed by high-pressure hot water extraction at 121°C for 15 minutes with 20 times the extraction solvent (purified water). The extracted cacao nibs extract was centrifuged to recover the extraction supernatant, and the extract was filtered through a filter having a size of 0.2 μm to prepare cacao nibs extracts, respectively. The extracted and filtered cacao nibs extract was used after lyophilization.

Thereafter, the fermentation broth of the sap of the Goro-sobensis and the cacao nibs extract were mixed in a ratio of 1:1 and used.

Comparative Example 3. Composition comprising fermentation broth of sap of Goro-sabi and pomegranate bark extract

After inflicting some wounds on the goryeosae trees that grow naturally in the Samsan Yugok, a clean container was attached to collect the naturally flowing sap to obtain pure Goro’s tree sap. Afterwards, yeast ( Saccharomyces cerevisiae ) bacteria were added to the collected sap of Goro-sae in an amount of 2 to 5 parts by weight based on 100 parts by weight of sap of Go-ro-sob and fermented at 40°C for 48 hours. Then, 30% of butylene glycol was added to the fermented raw material, extracted for 7 days at room temperature, filtered through a 400 mesh filter cloth, and filtered through a 0.45 μm filter to prepare a fermentation broth.

The pomegranate skin was washed with purified water, dried, and coarsely pulverized, followed by high-pressure hot water extraction at 121°C for 15 minutes with 20 times the extraction solvent (purified water). The extracted pomegranate peel extract was centrifuged to recover the extraction supernatant, and the extract was filtered through a filter having a size of 0.2 μm to prepare pomegranate peel extracts, respectively. The extracted and filtered pomegranate peel extract was used after lyophilization.

Thereafter, the fermentation broth and pomegranate peel extract of the sap of Goro-so-namu were mixed in a ratio of 1:1.

Comparative Example 4. A composition comprising sap of Goro serrata, cacao nibs extract, and pomegranate peel extract

After inflicting some wounds on the goryeosae trees that grow naturally in the Samsan Yugok, a clean container was attached to collect the naturally flowing sap to obtain pure Goro’s tree sap. Then, 30% content of butylene glycol was added to the sap, extracted for 7 days at room temperature, filtered through a 400 mesh filter cloth, and filtered through a 0.45 μm filter to prepare a goroso sap.

Each cacao nibs was washed with purified water, dried, and coarsely pulverized, followed by high-pressure hot water extraction at 121°C for 15 minutes with 20 times the extraction solvent (purified water). The extracted cacao nibs extract was centrifuged to recover the extraction supernatant, and the extract was filtered through a filter having a size of 0.2 μm to prepare cacao nibs extracts, respectively. The extracted and filtered cacao nibs extract was used after lyophilization.

The pomegranate skin was washed with purified water, dried, and coarsely pulverized, followed by high-pressure hot water extraction at 121°C for 15 minutes with 20 times the extraction solvent (purified water). The extracted pomegranate peel extract was centrifuged to recover the extraction supernatant, and the extract was filtered through a filter having a size of 0.2 μm to prepare pomegranate peel extracts, respectively. The extracted and filtered pomegranate peel extract was used after lyophilization.

Thereafter, the sap of the Goro-so tree, the cacao nibs extract, and the pomegranate peel extract were mixed in a ratio of 1:1 to 1 and used.

Experimental Example 1. Skin keratinocyte culture

HaCaT cells, which are skin keratinocytes, are Dr. Pre-sale from CG Hyun (Jeju National University, Korea) and using Dulbecco's Modified Eagle's Medium (DMEM) medium containing 100 units/ml penicillin-streptomycin and 10% fetal bovine serum (FBS) at 37°C, It was cultured in a 5% CO ₂ incubator, and subculture was performed every 3 to 4 days.

Experimental Example 2. Cytotoxicity evaluation (EZ-cytox assay)

In order to confirm the skin stability of Examples 1 to 5, cytotoxicity evaluation was performed using the EZ-cytox assay method. EZ-cytox assay is a representative method of measuring cell viability using the principle of producing orange water-soluble formazan by reacting with dehydrogenase of living cells.

Specifically, in order to confirm the toxicity in skin keratinocytes, HaCaT cells were dispensed into a 96-well plate at 5 X 10 ⁴ cells/mL using DMEM medium supplemented with 10% FBS, under conditions of 37°C and 5% CO _2. After reacting for 18 hours at, the absorbance was measured at 450 nm using a microplate reader. The average absorbance value for each sample group was obtained, and the cell viability was evaluated by comparing it with the absorbance value of the control group. The results are shown in Table 1.

Cell viability (% control) Concentration (ug/ml) 0 50 100 125 Example 1 100 115 122 129 Example 2 100 112 125 131 Example 3 100 112 122 132 Example 4 100 117 122 129 Example 5 100 112 125 131

As shown in Table 1, the compositions containing the fermentation broth, cacao nibs extract and pomegranate peel extract of Examples 1 to 5 all showed excellent cell viability regardless of concentration, and toxicity to skin keratinocytes. It was confirmed that the stability was high because there was little.

Experimental Example 3. Antioxidant effect confirmation

The antioxidant activity of Examples 1 to 5 and Comparative Examples 1 to 4 was confirmed. For the evaluation of antioxidant activity, DPPH (1,1-diphenyl1-2-picry1hydrazy1) was used to measure the free radical scavenging activity. Specifically, after adding 20 μL of each of the extracts of Examples 1 to 5 and Comparative Examples 1 to 4 to 180 μL of the DPPH solution, blocking the light from the outside and reacting at room temperature for 30 minutes, and then measuring the absorbance at 517 nm to measure the absorbance for the control group. The antioxidant activity was shown as a reduction ratio of. At this time, as a control for determining the rate of decrease in absorbance, ascorbic acid, an antioxidant, was used, and the same amount as the sample was added and measured in the same manner. Then, DPPH scavenging activity was calculated according to Equation 1 below.

[Equation 1]

DPPH scavenging activity (%) = [1-(absorbance of the sample treatment group in the Example or Comparative Example / absorbance of the sample untreated group)] X 100

division SC ₅₀ (μg/mL) Example 1 49.5 Example 2 50.1 Example 3 49.7 Example 4 51.0 Example 5 50.9 Comparative Example 1 58.5 Comparative Example 2 57.5 Comparative Example 3 56.9 Comparative Example 4 59.0 Control (vitamin C) 8.7

As a result, it was confirmed that the composition (Example 1) including the fermentation broth of the sap of the Goro-soburus sap, the cacao nibs extract and the pomegranate peel extract (Example 1) had overall excellent antioxidant activity. In particular, it was confirmed that the yeast fermentation broth of Goro-soburus sap can improve antioxidant efficacy by increasing the antioxidant activity compared to the cosmetic composition (Comparative Example 4) containing Goro-soburus sap, cacao nibs extract, and pomegranate bark extract.

3-2. ABTS free radical scavenging activity evaluation

The extracts of Examples 1 to 5 and Comparative Examples 1 to 4 were suspended in purified water to evaluate free radical scavenging activity.

In the ABTS assay, the antioxidant is blue-green radical ABTS ⁺ [2,2'-azino-beads (3-ethyl benzothiazoline-6-sulfonic acid (ABTS ⁺ [2,2'-Azino-bis(3-ethyl benzothiazoline-6-)). This is a method of measuring the degree of conversion to colorless ABTS by removing sulfonic acid)]) at 732 nm.

Vitamin C was used as the control group, and the concentration (SC ₅₀ ) for scavenging 50% of the radical ABTS was measured, and this was compared with the control group.

division SC ₅₀ (μg/mL) Example 1 38.2 Example 2 40.1 Example 3 39.5 Example 4 40.3 Example 5 40.3 Comparative Example 1 50.3 Comparative Example 2 50.1 Comparative Example 3 49.9 Comparative Example 4 52.5 Control (vitamin C) 8.7

As a result, it was confirmed that the antioxidant activity of the composition (Example 1) containing the fermentation broth of the sap of Goro-sera, cacao nibs extract, and pomegranate peel extract was significantly improved. In addition, compared to the composition containing the fermentation broth and cacao nibs extract (Comparative Example 2) or the fermentation broth and pomegranate bark extract (Comparative Example 3) of the fermented soybean sap, all of the fermented broth, cacao nibs extract, and pomegranate bark extract may be included. When it was confirmed that the antioxidant effect was particularly excellent.

The above results suggest that the antioxidant effect of the cosmetic composition including the fermentation broth of the sap of Goro-soburu, cacao nibs extract, and pomegranate peel extract is excellent.

Experimental Example 4. Anti-inflammatory effect confirmation

4-1. NO production inhibitory ability

For the measurement of NO production, the concentration of nitrite in the cell culture medium was used with a Grease reagent. RAW 264.7 cells were dispensed in a 12 well plate so that the number of cells was 1 × 10 ⁵ cells/well, and cultured with 80% confluence. Thereafter, the extracts of Examples 1 to 5 and Comparative Examples 1 to 4 were treated, respectively, and NO production was induced with LPS (100 ng/mL) for 24 hours, and then the culture supernatant and the grease reagent were mixed at 1:1 to ELISA. NO production was measured at 540 nm using a reader. After preparing a calibration curve using sodium nitrite (NaNO ₂ ), it was used for NO measurement. The amount of NO produced as a percentage is shown in Table 4 below.

The lower the NO generation rate is, the better the NO generation inhibitory ability is, so it can be evaluated as having excellent anti-inflammatory effect.

division NO generation rate (%) Example 1 44.5 Example 2 45.0 Example 3 44.8 Example 4 46.0 Example 5 45.5 Comparative Example 1 51.7 Comparative Example 2 50.5 Comparative Example 3 50.3 Comparative Example 4 53.5 Positive control 30.1 Negative control (no treatment) 100.0

As a result, the composition (Example 1) containing the fermentation broth of the Sap of Goro serrata, the cacao nibs extract and the pomegranate bark extract (Example 1) suppressed NO production compared to the cosmetic composition (Comparative Example 4) containing the Sap of Goro serrata, the cocoa nibs extract and the pomegranate bark extract. It was confirmed that the effect was further improved.

Through the above results, it can be seen that the cosmetic composition comprising the fermented broth of the sap of Goro-soburus sap, the cacao nibs extract, and the pomegranate peel extract has excellent efficacy in improving inflammation.

Experimental Example 5. Evaluation of dermatitis improvement effect

The effect of improving dermatitis of the cosmetic compositions including Examples 1 to 5 and Comparative Examples 1 to 4 was evaluated. Each composition was formulated as shown in Table 5 below.

Raw material name Content (% by weight) Cetearyl alcohol 2 Cetearyl glucoside One Jojoba seed oil 5 Silica 0.5 glycerin 10 Examples 1 to 5/Comparative Examples 1 to 4 3 to 4 Purified water To 100

For 50 selected women from 20 to 40 years old, the cosmetic compositions of Examples 1 to 5 and Comparative Examples 1 to 4 were used for 4 weeks in the same way as a conventional product, and improved according to the user's opinion. The degree was judged.

division 1 week 2 weeks 3 weeks 4 weeks Example 1 +++ ++ + ± Example 2 +++ ++ ++ ± Example 3 +++ +++ ++ ± Example 4 +++ ++ + ± Example 5 +++ ++ ++ ± Comparative Example 1 ++ + ± ± Comparative Example 2 ++ + ± ± Comparative Example 3 ++ + ± ± Comparative Example 4 + ± ± ±

<Evaluation criteria>

+++: Has a very good improvement effect / ++: Has a significant improvement effect / +: Has a slight improvement effect / ±: No improvement effect, but does not worsen / -: No improvement effect, rather weakens

As shown in Table 6, it was confirmed that the essence formulation (Examples 1 to 5) comprising the fermentation broth of the sap of Goro-sabi, cacao nibs extract, and pomegranate peel extract has excellent dermatitis improvement effects compared to the essence formulations of Comparative Examples 1 to 4. I did.

Preparation Example 1. Preparation of an essence containing a cosmetic composition containing the fermented broth, cacao nibs extract, and pomegranate peel extract of the sap of the Goro-sabi of the present invention

A cosmetic composition of an essence formulation was prepared as shown in Table 7 below (unit: wt%).

Raw material name Content (% by weight) Cetearyl alcohol 2 Cetearyl glucoside One Jojoba seed oil 5 Silica 0.5 glycerin 10 Examples 1 to 5 3 to 4 Purified water To 100

Preparation Example 2. Preparation of a cream containing a cosmetic composition containing the fermented broth, cacao nibs extract, and pomegranate peel extract of the sap of Goro-sabi of the present invention

As shown in Table 8 below, a cream-formed cosmetic composition was prepared (unit: wt%).

Composition Content (% by weight) Examples 1 to 5 3 to 4 Stearic acid 15 ethanol One Potassium hydroxide 0.7 glycerin 5 Propylene glycol 3 antiseptic Appropriate amount incense Appropriate amount Purified water to 100

Preparation Example 3. Preparation of a mask pack containing a cosmetic composition comprising the fermented broth, cacao nibs extract, and pomegranate peel extract of the sap of Goro-so-tree of the present invention

A mask pack was prepared using the cosmetic composition as shown in Table 9 below (unit: wt%).

Composition Content (% by weight) Examples 1 to 5 3 to 4 Polyvinyl alcohol 15 Cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Cyclomextrin 0.15 DL-Panlenol 0.4 Allantoin 0.1 Monoammonium glycyrrhizinate 0.3 Nicotinamide 0.5 ethanol 6.0 PEG 40 hydrogenated castor oil 0.3 Preservatives, fragrances, and pigments Appropriate amount Distilled water to 100

The above description of the present invention is for illustrative purposes only, and those of ordinary skill in the art to which the present invention pertains can understand that it is possible to easily transform it into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as being distributed may also be implemented in a combined form.

The scope of the present invention is indicated by the claims to be described later, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be interpreted as being included in the scope of the present invention.

Claims (9)

As a cosmetic composition for improving skin inflammation, comprising a fermented broth of a sap of Goro serrata, a cacao nibs extract, and a pomegranate peel extract,
The fermentation broth of the sap of the Goro-so-tree sap is fermented by inoculating yeast of the genus Saccharomyces . delete The method of claim 1,
The yeast is Saccharomyces cerevisiae , Saccharomyces byanus , Saccharomyces uvarum , Saccharomyces pastorianus , and Saccharomyces pastorianus . Su ( Saccharomyces paradoxus ) is any one or more selected from the group consisting of, a cosmetic composition. The method of claim 1 or 3,
The cosmetic composition will further include an antioxidant use, cosmetic composition. The method of claim 1 or 3,
The cosmetic composition is formulated with one or more selected from the group consisting of softening lotion, nutrient lotion, nutrition cream, massage cream, essence, ampoule, gel, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray, and powder. Cosmetic composition. As a food composition for improving skin inflammation, comprising a fermented broth of a sap of Goro serrata, a cacao nibs extract, and a pomegranate peel extract,
The fermentation broth of the sap of the Goro-so tree is fermented by inoculating yeast of the genus Saccharomyces . delete The method of claim 6,
The yeast is Saccharomyces cerevisiae , Saccharomyces byanus , Saccharomyces uvarum , Saccharomyces pastorianus , and Saccharomyces pastorianus . Su ( Saccharomyces paradoxus ) is any one or more selected from the group consisting of, food composition. The method according to claim 6 or 8,
Wherein the food composition further comprises an antioxidant use, food composition.