KR101821024B1 - COMPOSITION OF SKIN WHITENING CONTAINING EXTRACT OF Gloiopeltis Furcata AND METHOD OF MAKING THE SAME - Google Patents
COMPOSITION OF SKIN WHITENING CONTAINING EXTRACT OF Gloiopeltis Furcata AND METHOD OF MAKING THE SAME Download PDFInfo
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- KR101821024B1 KR101821024B1 KR1020150186840A KR20150186840A KR101821024B1 KR 101821024 B1 KR101821024 B1 KR 101821024B1 KR 1020150186840 A KR1020150186840 A KR 1020150186840A KR 20150186840 A KR20150186840 A KR 20150186840A KR 101821024 B1 KR101821024 B1 KR 101821024B1
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
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Abstract
The present invention relates to a whitening composition comprising a unglazed extract of Phragmites fungi and a method of preparing the same, wherein the whitening composition comprising the unglazed extract of Fagaceae according to the present invention comprises an extract of Gloiopeltis Furcata, It can be an effect.
The whitening composition comprising the unsweetened pulpy powder extract according to the present invention can improve skin whitening activity by inhibiting melanin production without causing side effects due to no cytotoxicity. Therefore, by using the composition for whitening comprising the unglazed flower fly extract, it can be provided as a variety of products or formulations having excellent whitening effect without side effects.
Description
The present invention relates to a composition for whitening comprising an extract of Phragmites fungi and a method of producing the same. More particularly, the present invention relates to a composition for whitening, which comprises an extract of unglazed granite and has no side effects and has a high whitening activity, and a method for producing the same.
Several factors are involved in determining the skin color of a person. Among them, factors such as the activity of the melanocyte making the melanin pigment, the distribution of the blood vessel, the thickness of the skin, and the presence or absence of the pigment in and out of the human body such as carotenoid and bilirubin It is important.
Especially, the most important factor is melanin, a melanin pigment produced by the action of various enzymes such as tyrosinase in melanocytes in the human body. The formation of melanin pigment affects genetic factors, hormonal secretion, physiological factors such as stress, and environmental factors such as ultraviolet irradiation.
The melanin pigment produced in melanocytes of body skin is a phenolic polymer substance having a complex form of black pigment and protein. It protects the subcutaneous skin organs by blocking ultraviolet rays irradiated from the sun, and at the same time, free radicals And protects proteins and genes in the skin.
Melanin, which is produced by stress stimuli inside and outside of the skin, is a stable substance that does not disappear until the skin is excreted through skin keratinization even if the stress disappears. However, when melanin is produced more than necessary, it induces hypercholesterolemia such as spots, freckles, and dots, resulting in poor cosmetic results. In addition, as the number of people enjoying outdoor activities increased due to the increase in the leisure population, the demand for preventing melanin pigmentation due to ultraviolet rays increased.
In response to such demands, substances having ascorbic acid, kojic acid, arbutin, hydroquinone, glutathione, derivatives thereof, or tyrosinase inhibiting activity have been conventionally used in cosmetics or pharmaceuticals, The use thereof is limited due to a whitening effect, a safety problem to the skin, a problem of formulation and stability in combination with cosmetics, and the like.
Accordingly, development of a substance which shows excellent efficacy of whitening on the human body and safety is demanded, and a whitening composition based on a natural extract having a small side effect has been attracting attention, and various studies related thereto have been carried out .
Prior art 1 (KR 10-2012-0028536 A, public date: March 23, 2012) refers to fruit pulverization products formed by pulverizing fruit having a moisture content of 85% or more and a sugar content of 7 Brix or more, water, A cosmetic composition for whitening, characterized in that the extraction mixture containing a food or medical hydrolytic enzyme is subjected to an enzymatic hydrolysis treatment to contain at least 25 μg / g of an organic acid enhanced by oxalic acid and malic acid And a fruit extract for manufacturing.
The prior art 2 (KR 10-2007-0089327 A, publication date: August 31, 2007) contains a herb plant ingredient such as yallow, bamboo mint, and peppermint together with a vitamin C derivative which is a typical antioxidant, Discloses a cosmetic composition for skin whitening that contains citric acid and sodium citrate as a pH buffer to maintain the pH in the range of 6 to 7 to stabilize the vitamin C derivative to maintain the whitening effect for a long time.
However, in order to develop a whitening composition having high whitening effect and no side effects, research on a variety of natural products is required.
It is an object of the present invention to provide a whitening composition comprising an extract of Phragmites fungi having an effect of inhibiting melanin production without cytotoxicity and without causing side effects.
Another object of the present invention is to provide a functional product, such as a whitening food, prepared by using the whitening composition containing the unsweetened whole flower extract.
To achieve the above object, the present invention provides a whitening composition comprising an extract of Glochopteris Furcata, which has a skin whitening effect.
The extract may be one selected from the group consisting of water, alcohols having 1 to 10 carbon atoms, hexane, chloroform, ethyl acetate, and mixtures thereof.
The whitening composition comprising the unsweetened pulpy extract may have a viscosity of 1.2 to 100 cP.
The above-mentioned pollen grains are selected from the group consisting of Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum bifidobacterium, Bifidobacterium breve bifidus bacterium, and Lactobacillus bacterium. And Lactobacillus acidophilus. In addition, it may be fermented by inoculating at least one selected from the group consisting of Lactobacillus acidophilus.
The whitening composition comprising the unsweetened pulpy powder extract may further comprise a juniper hot water extract.
The whitening composition comprising the unsweetened pulpy powder extract may further comprise a Bacillus thuringiensis extract.
The cosmetic composition according to another embodiment of the present invention may include a whitening composition comprising the unglazed flower fly extract.
The food composition according to another embodiment of the present invention may include a whitening composition comprising the unsweetened pulpy powder extract.
The method for preparing a whitening composition according to still another embodiment of the present invention comprises the steps of cutting water to a predetermined size, filtering water, an alcohol having 1 to 10 carbon atoms, hexane, chloroform, ethyl acetate And a mixture thereof to a solvent to prepare a solvent mixture; Introducing an inert gas into the reaction mixture while maintaining the temperature of the reaction mixture at a temperature of 15 to 75 DEG C to adjust the pressure in the reactor to 20 to 35 atm; A discharging step of discharging the solvent together with the inert gas in the reactor after the reaction step to produce a granulated pollen pulp extract; And a mixing step of mixing the granular extract with the selected one selected from the group consisting of hot spring water extract of Jodo orchardgrass, glutinous hot water extract and mixtures thereof.
Hereinafter, the present invention will be described in more detail.
The whitening composition comprising the extract of Phragmites fungi according to an embodiment of the present invention may contain a Gloiopeltis Furcata extract and may have a skin whitening effect.
The scientific name of the Abelmoschus eryngii is Gloiopeltis Furcata (Postels et Ruprecht) J. and belongs to Rhodophyta> Rhodophyceae> Gigartinales> Endocladiaceae.
The stem of the disintegration flask is circumferential. The branch is irregularly Y-shaped, branch branches are narrow and the body looks like a joint, the stem is short and the morphology is severe. It is thin at the forking point, rounded or pointed at the top, vagina is like a thin leather, and is mucus-rich. It is also similar to the outer shape of the pulsar, but the stems are hollow and can be distinguished.
In addition, the unglazed pulses are attached to rocks or stones at the upper part of the intertidal zone, and several individuals are generated in one place, and they are inhabited. From late March to April, spores appear, and after the first flourishes to early summer, the upright bodies that have released the spores die, and the released spores soon germinate by attaching to others and germinating to create new upright bodies in the autumn and the following spring Repeat the growth activity. It is used as a raw material for the food to be fed into the fabric or it is edible such as bibimbap, The unstained pulsatillae has recently been spotlighted by the anticancer effect, and it is inhabited in the southern coast of Korea.
Preferably, the unsweetened pulpy powder extract may inhibit the activity of tyrosinase.
That is, the whitening effect can be understood as a concept including inhibition of melanin production and inhibition of the activity of tyrosinase, an enzyme that plays a key role in the melanin synthesis process.
Melanin is a widely distributed pigment in organisms and is synthesized in melanocytes in the epidermis of the human body. The major enzyme that acts on the melanin biosynthesis process is tyrosinase. Tyrosinase is a type of polyphenol oxidase that contains copper and oxidizes tyrosine at melanocytes to produce L-3,4-dihydroxyphenylalanine (L-3,4-dihydroxyphenylalanine, DOPA ), DOPA is oxidized again and converted to dopaquinone, and then is subjected to a non-enzymatic oxidation reaction.
Therefore, it is possible to inhibit the activity of the tyrosinase enzyme and inhibit the production of melanin to have a skin whitening effect.
Therefore, the unsweetened Phragmites extract inhibits the activity of tyrosinase, an enzyme that plays a key role in the melanin synthesis process, and thus has a high whitening effect.
The extract may be one selected from the group consisting of water, alcohols having 1 to 10 carbon atoms, hexane, chloroform, ethyl acetate, and mixtures thereof.
Preferably, the extract may be an alcoholic extract having 1 to 10 carbon atoms, and the unbranched polysaccharide and the alcohol having 1 to 10 carbon atoms may be mixed in a weight ratio of 1:10 to 10: 1. When the alcohol is used, it is useful for extracting the whitening active ingredient. When the alcohol is used, it may be suitable for extracting the whitening active ingredient contained in the unglazed flower lacquer.
More preferably, the unbranched sugarcane and the carbonated ethanol may be mixed in a weight ratio of 1: 7 to 1: 1. In the above range, the yield of the whitening active ingredient contained in the unglazed flower can be increased.
Preferably, the unsweetened pulpy powder extract may contain 50 to 500 mg / l. The above range can be used without any side effects because of little cytotoxicity. However, if the extract of Phytophthora balsameroa is more than 500 mg / l, the cytotoxicity is increased and a side effect such as skin irritation may occur.
In addition, due to the lowering of activity against tyrosinase in the above range, the whitening effect can be excellent, and there is no cytotoxicity, and thus it has an advantage that it can be safely used without side effects.
The above range may be suitable for use as a composition having a high whitening effect without side effects according to the present invention.
The whitening composition comprising the unsweetened pulpy extract may have a viscosity of 1.2 to 100 cP.
According to the viscosity range, the flavor and texture can be enhanced because the flavor is felt by the viscosity range. The whitening composition containing the unsweetened pulpy extract must have a high texture and flavor for the continuous administration of the whitening composition.
In order to adjust the viscosity, the whitening composition comprising the unsweetened pulpy extract may further comprise a thickening agent. The thickener is allowed to be used as a food, and is considered to include a thickener that can be used by those skilled in the art.
Preferably, the whitening composition comprising the unsweetened pulpy extract may have a viscosity of 1.2 to 10 cP. According to the above range, the texture and flavor are very good, which can be helpful for steady consumption for a certain period of time.
The above-mentioned pollen grains are selected from the group consisting of Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum bifidobacterium, Bifidobacterium breve bifidus bacterium, and Lactobacillus bacterium. And Lactobacillus acidophilus. In addition, it may be fermented by inoculating at least one selected from the group consisting of Lactobacillus acidophilus.
The upper leaf extract may be one extracted using a fermented upper leaf. When the fermented leaves are used, they are advantageous in that they can be used stably at a relatively high concentration because of low cytotoxicity.
Preferably, the pollen grains may be inoculated with Lactobacillus casei, Lactobacillus rhamnosus. The cytotoxicity can be lowered by the microbial fermentation, and when the unsweetened pulpy flour is fermented according to the fermentation process by inoculation with the microorganism, the effective substance for whitening activity is increased and the whitening effect Can be increased.
The whitening composition comprising the unsweetened pulpy powder extract may further comprise a juniper hot water extract.
The whitening composition comprising the unsweetened pulpy powder extract may further comprise a Bacillus thuringiensis extract.
In the present specification, the term 'Bok Jo-hui grass' is a deciduous shrub belonging to the buttercup butterfly. The leaf is a three-folded leaf composed of three small leaves. The small leaf is wide egg-shaped, and two of the three are next to each other. The leaves grow larger as they go up to the top, with sharp ends and coarse teeth, with slightly reddish ridged sawtooth on the edge, but are often divided into three shallowly.
When used in combination with the above-mentioned paste, the whitening effect is higher, and it is effective in improving wrinkles. However, it is necessary to remove the toxicity through the fermentation process because the bacteriocin has its own toxicity.
Preferably, the baculovirus full extract may be a full-fermented bacillus subtilis extract prepared by a fermentation extraction method. Since the bacillus grass is weakly toxic, it is necessary to remove the toxicity through the fermentation process.
The full fermentation extract of Bacillus thuringiensis according to the present invention is prepared by adding 1 to 3 parts of saccharide of skin and 0.05 to 0.25 part of sun-dried salt of skin to 100 parts of rice skin, adding 0.5 to 2.0 parts of skin microbial solution The whole pulverized product can be obtained by immersing the pulverized product in an amount of 10 to 30 parts by volume and then fermenting for 3 to 15 days in a room temperature atmosphere which is shielded from air.
The microorganism may be selected from the group consisting of Lactobacillus casei, Lactobacillus rhamnosus, Bifidobacterium bifidum bifidobacterium, Bifidobacterium breve bifidobacterium, and Lactobacillus sp. Lactobacillus acidophilus, and Bacillus acidophilus.
The juniper tree to be used in the present invention is also referred to as 杜松 (杜松), and belongs to the lateral buds. Leaves are narrow and narrow linear needle leaves, each of which rotates in three, with a length of 12 ~ 20mm, a pointed end, and a narrow white groove at the center of the surface.
Preferably, the juniper can be fermented in the same manner as the bovine juice paste. When fermenting the juniper, the effect of removing the unique fragrance of the unglazed pulses is high and the flavor can be enhanced.
As used in the present invention, 'kangsak' is a perennial plant belonging to the rose family. Height is about 30 to 100 centimeters, stem grows out from coarse rootstock, and hair is whole. Leaves are alternate phyllotaxis, 5 ~ 7 small leaves. In June ~ August, a yellow flower blooms in total sprout, and the fruit has thorny hairs and sticks to other things.
The cosmetic composition according to another embodiment of the present invention may include a whitening composition comprising the unglazed flower fly extract.
The cosmetic composition of the present invention may include, without limitation, commonly accepted ingredients other than the above-mentioned effective ingredients, and may contain conventional additives such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavoring agents, have.
The cosmetic composition according to the present invention can be used as a cosmetic composition in the form of a solution, an ointment for external use, a cream, a foam, a nutritional lotion, a softening water, a pack, a soft water, an emulsion, a makeup base, But are not limited to, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansers, oils, powder foundations, emulsion foundations, wax foundations, patches and sprays.
In addition, the cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a general skin cosmetic composition, and examples thereof include oil, water, a surfactant, a moisturizer, A thickening agent, a chelating agent, a coloring matter, an antiseptic, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto. The cosmetically acceptable carrier to be contained in the cosmetic composition of the present invention varies depending on the formulations.
When the formulation of the present invention is an ointment, a paste, a cream or a gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide Mixtures of these may be used.
When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as the carrier component, Propellants such as fluorohydrocarbons, propane / butane or dimethyl ether.
When the formulation of the present invention is a solution or emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil may be used, in particular fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycols or sorbitan may be used have.
When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.
When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, glycerol, .
When the formulation of the present invention is an interface-active agent-containing cleansing, the carrier component is selected from the group consisting of aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.
The food composition according to another embodiment of the present invention may include a whitening composition comprising the unsweetened pulpy powder extract.
The food composition of the present invention includes a health functional food, and the kind thereof is not particularly limited. Examples of the health functional food to which the extract can be added include dairy products such as meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen and other noodles, gums, ice cream, Alcoholic beverages, and vitamin complexes, and may include all the health functional foods in the conventional sense, and foods used as feeds for animals. In addition to the above, the health functional food composition of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, , Alcohols, carbonating agents used in carbonated drinks, and the like. It may also contain flesh for the production of natural fruit juices, fruit juice drinks and vegetable drinks.
The method for preparing a whitening composition according to still another embodiment of the present invention comprises the steps of cutting water to a predetermined size, filtering water, an alcohol having 1 to 10 carbon atoms, hexane, chloroform, ethyl acetate And a mixture thereof to a solvent to prepare a solvent mixture; Introducing an inert gas into the reaction mixture while maintaining the temperature of the reaction mixture at a temperature of 15 to 75 DEG C to adjust the pressure in the reactor to 20 to 35 atm; A discharging step of discharging the solvent together with the inert gas in the reactor after the reaction step to produce a granulated pollen pulp extract; And a mixing step of mixing the granular extract with the selected one selected from the group consisting of hot spring water extract of Jodo orchardgrass, glutinous hot water extract and mixtures thereof.
The solid obtained in the step of discharging is rapidly cooled at a temperature of 10 ° C or lower and is mixed with any one selected from the group consisting of the hot-water extract of the juniper fruit, the hot water extract of Bacillus subtilis, and the mixture thereof to adjust the concentration of the unglacial extract Lt; / RTI >
By the rapid cooling, it is possible to form nano-sized particles having a relatively large surface area and high solubility without loss of an active ingredient, so that not only the dispersibility to the composition is high but also the absorption rate in the body during oral administration can be further increased, When used as an external agent, it has an advantage of being easily penetrated into skin barrier.
The whitening composition comprising the unsweetened pulpy powder extract according to the present invention can improve skin whitening activity by inhibiting melanin production without causing side effects due to no cytotoxicity. Therefore, by using the composition for whitening comprising the unglazed flower fly extract, it can be provided as a variety of products or formulations having excellent whitening effect without side effects.
In addition, based on the effect of the whitening composition containing the unglazed flower extract, a functional product such as a whitening food prepared by using the whitening composition containing the unglazed flower extract can be provided.
Fig. 1 shows the effect of Fusarium oxysporum extract on the survival rate of B16F10 melanoma cells.
FIG. 2 is a graph showing the effect of fermented unsweetened pulp extract on the survival rate of B16F10 melanoma cells.
FIG. 3 is a graph showing the effect of the extract on the intracellular Tyrosinase activity.
Fig. 4 shows the effect of Fusarium oxysporum extract on melanin production of B16F10 melanoma cells.
FIG. 5 is a graph showing the effect of Fusarium oxysporum extract on protein expression of melanin synthesis-related enzymes.
Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
[Production Example: Preparation of Samples]
One. Fulgas Preparation of extract
In order to confirm the effect of the present invention, Gloiopeltis Furcata ethanol extracts (GFEE) were prepared using ethanol.
2. Preparation of mixed extract
The fermentation broth extract B, which was fermented with the above extracts A and Lactobacillus casei, was prepared.
The hot water extract (Extract C), the hydrothermal extract (Extract D), the water extract (Extract E), and the hot water extract of mung bean Extract F) was prepared.
The extracts were then mixed according to the following Table 1 to prepare Examples.
[ Experimental Example : Fulgas Test method for extract]
1. Reagents and antibodies
Tyrosinase, MITF, TRP1, TRP2 and actin antibodies used for protein analysis were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA) and Cell Signaling Technology (Beverly, MA, USA). Peroxidase-labeled donkey anti-rabbit and peroxidase-labeled sheep anti-mouse immunoglobulins used as secondary antibodies for immunoblotting were purchased from Amersham Life Science Corp. (Arlington Heights, Ill., USA). α-Melanocyte stimulating hormone (α-MSH) and L-DOPA were purchased from Sigma-Aldrich (St. Louis, Mo., USA).
2. Cell line and cell culture
B16F10 mouse melanoma cells were purchased from Korean Cell Line Bank and DMEM (WelGENE, Daegu, Korea) supplemented with 10% fetal bovine serum (WelGENE, Daegu, Korea) and 1% penicillin-streptomycin (Gibco BRL) Lt; 0 > C, 5% CO2. B16F10 cells were incubated with trypsin (Hyclone, USA) in order to eliminate overcorrection caused by cell proliferation.
3. Of cell viability Measure
B16F10 melanoma cells were seeded at 3 × 10 4 / ml in a cell culture 6 well plate and treated with a non-adherent extract of Phragmites carrageenum at an appropriate concentration per well. After 72 hours, 200 μl of tetrazolium bromide salt (MTT, Amresco, Solon, OH, USA) diluted with 0.5 mg / ml was added to each well and incubated in a CO 2 incubator for 2 hours. The medium and MTT reagent were removed and
4. B16F10 Measurement of melanin production in melanoma cells
B16F10 cells were treated with α-melanocyte stimulating hormone (α-MSH) and SHEE for 3 days, and the cells were collected and washed twice with phosphate-buffered saline (PBS). After incubation for 1 hour at 80 ° C with 1N NaOH containing 10% dimethylsulfoxide (DMSO, Ameresco, Solon, Ohio, USA), the absorbance at 475 nm was measured with an ELISA reader (Molecular Devices, Sunnyvale, Respectively.
5. B16F10 melanoma cell tyrosinase Active measurement
B16F10 cells were treated with α-MSH and SHEE for 3 days. Cells were collected and lysed by adding 1% Triton X-100, 0.1M PMSF for 1 hour to dissolve the cells. After centrifugation And the supernatant was collected. L-DOPA (mg / ml) was added, and the absorbance was measured at 420 nm while observing the change of absorbance at 1 hour.
6. Analysis of protein expression by Western blot analysis
B16F10 mouse melanoma cells prepared in the same manner were added with an appropriate amount of lysis buffer (1% Triton X-100, 0.1M PMSF), reacted at 4 ° C for 1 hour, and centrifuged at 14,000 rpm for 30 minutes. Total protein was isolated. The protein concentration of the supernatant was quantitated according to the Bio-Rad protein assay reagent (Bio-Rad, Hercules, Calif., USA) and the method of use, and then mixed with an equal volume of Laemmli sample buffer (Bio-Rad). Samples of the same amount were separated by electrophoresis using sodium dodecyl sulphate (SDS) -polyacrylamide gel and electroblotted with PVDF membrane (Bio-Rad, USA). The PVDF membrane with the separated proteins was treated with 5w% skim milk to block the nonspecific proteins. The primary antibody was treated and then over-night at room temperature or at 4 ° C, followed by washing with PBS-T (4 times for 10 minutes) and reacted for 1 hour at room temperature using a secondary antibody (diluted 1: 1500 with PBS-T) to the treated primary antibody. After the reaction was completed, the Enhanced Chemiluminoesence (ECL) solution (Amersham Life Science Corp.) was applied to the dark room and then exposed to X-ray film to analyze the amount of specific protein expressed.
7. Statistical analysis
All experimental results were expressed as mean ± SD using the Statistical Package for the Social Sciences (SPSS) statistical program. Statistical significance of the analytical items in each experimental group was verified at the p <0.05 level using Duncan's multiple range test.
[Experimental Example: Test Results on Phyllostachys Extracts]
One. Fulgas The extract B16F10 Effect on melanoma cell survival rate
The cytotoxicity of B16F10 melanoma cell extracts was investigated to determine the effect of B16F10 melanoma cell survival. The results are shown in FIG. As a result, it was confirmed that the treatment with 50 ~ 1,000 μg / ml of ethanol extract of unglazed pulsatillae showed no cytotoxicity up to 400 μg / ml.
On the other hand, as shown in FIG. 2, the cell survival rate was significantly higher when using the Fusarium oxysugarifera extract, and it was confirmed that the survival rate could be maintained at a relatively high level even at 1000 μg / ml. Therefore, it is advantageous that the extract is free from side effects because of low cytotoxicity.
2. Fulgas The extract Tyrosinase Effect on activity
The activity of tyrosinase, which plays a key role in the synthesis of melanin, was investigated in order to investigate the inhibition of tyrosinase activity. The experiment was carried out in the same concentration range with reference to the MTT results. The results are shown in FIG. As shown in FIG. 3, the extracts of Fusarium oxysporum were treated with 50-200 μg / ml of Fusarium oxysporum fusiforme, respectively, and the concentrations of Fusarium oxysporum were 0.3%, 35%, 59% and 81% I could confirm. Therefore, it seems that the extract of Fusarium oxysporum has an inhibitory effect on tyrosinase activity induced by α-MSH.
3. Fulgas The extract B16F10 Melanin production in melanoma cells
The amount of synthesized melanin was measured after treatment with Phragmites crassifolia extract. The experiment was carried out in the same concentration range as in the tyrosinase activity measurement experiment, and the results are shown in FIG. The experiment was carried out in the same concentration range as the experiment. Melanogenesis was significantly increased in the α-MSH treated group compared with the control group. The treatment with the 50 μg / ml μg / ml extract of Fusarium oxysporum was 56%, 61%, 72% %, And 73%, respectively. In addition, when the melanin pigmentation was visually observed, only the α-MSH treatment showed a dark color, and melanin color decreased in the treatment group when the unglazed extract of Phyllostachys mellifera was treated in combination. Therefore, the extract of Fusarium oxysporum showed an inhibitory effect on α-MSH-induced melanin production.
4. Fulgas Effect of Extracts on Protein Expression of Melanin Synthesized Enzymes
The expression of MITF, TRP-1, TRP-2, and tyrosinase was inhibited by Western blot in order to confirm the inhibition of tyrosinase activity and melanin production induced by α-MSH stimulation. And the results are shown in Fig. 5 below. When α-MSH was treated alone, the expression of each protein was markedly increased, whereas when the extract of Phragmites pulverus was treated, the concentration-dependent decrease was observed. Especially, at the concentration of 200 μg / ml, the inhibition was remarkably suppressed and the same results as those of the previous experiments were confirmed.
5. Whitening and moisturizing performance evaluation of compound extract
The spray-type essence was prepared using the above combined extracts T1 to T10. The spraying-type essence was evaluated by an index of 1 to 10 with respect to whitening performance, moisturizing satisfaction, skin stability and flavor (flavor) 2]. (The higher the number, the better the performance.) The panel was composed of 20 males and 20 females, aged 20 to 30, respectively.
(Unit: index)
Referring to Table 2, it was confirmed that the whitening activity was better when the fermented product was used than when the extract was used alone.
Furthermore, when combined extracts were used, the whitening activity was increased, and the moisturizing performance, skin stability and flavor were improved.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.
Claims (9)
The unsweetened pulp flour was fermented with Lactobacillus casei,
For 100 parts by weight of the unsweetened pulpy powder extract
20 to 80 parts by weight of a hot-water extract of juniper fruit fermented with rice flour;
20 to 80 parts by weight of a hot water extract of Bacillus subtilis fermented with rice flour is contained,
Skin whitening effect
A whitening composition comprising a pulverulent extract.
The whitening composition comprising the unsweetened pulpy powder extract
And a viscosity of 1.2 to 100 cP
A whitening composition comprising a pulverulent extract.
Cosmetic composition.
Introducing an inert gas into the reaction mixture while maintaining the temperature of the reaction mixture at a temperature of 15 to 75 DEG C so that the pressure in the reactor is 20 to 35 atm;
A discharging step of discharging the solvent together with the inert gas in the reactor after the reaction step to produce a granulated pollen pulp extract; And
And a mixing step of preparing a mixed extract by mixing the above granulated pollen pulp pulp extract with hot water extract of juniper seeds fermented with rice rice and fermented hot water extract of rice bran fermented with rice flour,
Wherein the mixing step comprises mixing 20 to 80 parts by weight of hot water extract of juniper fruit fermented with 100 parts by weight of the unsweetened pulpy powder extract, And 20 to 80 parts by weight of a hot water extract of Botrytis cinerea fermented with rice flour
A method for preparing a whitening composition,
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| KR20200024464A (en) * | 2018-08-28 | 2020-03-09 | (주)월드코스텍 | Antioxidation and whitening active composition containing complex seaweed fermented extract |
| KR20230104514A (en) | 2021-12-31 | 2023-07-10 | 이주운 | Bioconverted Polysaccharide Extract of Gloiopeltis furcata with High Anti-inflammation Activity, and Use thereof |
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| KR102099251B1 (en) * | 2018-08-22 | 2020-04-08 | 주식회사 씨앤비코스메틱 | Manufacturing algae fermented complex using lactatobacillus sakei and cosmetic composition containing the same |
| KR102062006B1 (en) | 2019-07-22 | 2020-01-03 | 엘앤피코스메틱 (주) | Cosmetic composition and manufacturing method thereof |
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| JP2014185140A (en) * | 2013-01-18 | 2014-10-02 | Ina Food Industry Co Ltd | Melanin-producing cell-growth inhibitor and external preparation for skin containing the same |
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| KR20200024464A (en) * | 2018-08-28 | 2020-03-09 | (주)월드코스텍 | Antioxidation and whitening active composition containing complex seaweed fermented extract |
| KR102123588B1 (en) * | 2018-08-28 | 2020-06-16 | (주)월드코스텍 | Antioxidation and whitening active composition containing complex seaweed fermented extract |
| KR20230104514A (en) | 2021-12-31 | 2023-07-10 | 이주운 | Bioconverted Polysaccharide Extract of Gloiopeltis furcata with High Anti-inflammation Activity, and Use thereof |
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