KR102062006B1 - Cosmetic composition and manufacturing method thereof - Google Patents

Abstract

The present invention provides a cosmetic composition comprising 1.5 to 5.0 wt% of a Gloiopeltis tenax extract, 0.1 to 1.5 wt% of polyamine derived from plants, 0.01 to 0.5 wt% of hinokitiol, and a remainder consisting of a solvent. Furthermore, the present invention provides a method for manufacturing a cosmetic composition, comprising the following steps: preparing a first composition comprising a Gloiopeltis tenax extract and a first solvent; preparing a second composition comprising plant-derived polyamine, hinokitiol, and a second solvent; and mixing the first and second compositions, wherein the second solvent includes a polyhydric alcohol compound.

Description

Cosmetic composition and manufacturing method thereof

The present invention relates to a cosmetic composition and a method for producing the same.

The skin is the largest organ in the body, and is divided into three layers of epidermis, corium, and subcutaneous fat tissue in order from the outside. Protects against

In particular, the skin contains 65 to 70% of the body's total moisture, this moisture carries a variety of biologically active substances necessary for the human body, helping to maintain a soft and moist skin.

The epidermis plays an important role in controlling the evaporation of this moisture out of the body. In addition, the epidermis is separated from the outside in the order of the stratum corneum, granule layer, polar layer, basal layer, the cells of the stratum corneum acts like a brick and the intercellular lipids between the stratum corneum acts as a mortar to form a skin barrier. (J. Invest. Dermatol. 80 (Suppl.), 44-49. 1983).

Recently, in order to maintain the moisture of the skin, not only to increase the moisture in the stratum corneum, but also the differentiation process that develops from the basal keratinocytes to the outermost stratum corneum cells must be performed normally to act as a skin retention barrier. It turns out that it can be performed. In other words, physiologically, skin dryness is due to prolonged dropout time of the stratum corneum or degradation of lipid synthesis ability of the epidermal cells, and a decrease in the amount of moisturizing factors and lipids of the stratum corneum. Can promote it. Therefore, the use of a water retaining substance in the stratum corneum and a substance that serves to promote keratin differentiation may provide a better skin moisturizing effect.

As a prior art, Korean Patent No. 10-1712479 discloses a composition for alleviating scalp irritation containing full starch extract, and Korean Patent Laid-Open Patent No. 10-2017-0076972 discloses a whitening composition containing ternary starfish extract. However, the prior art documents described for the skin moisturizing cosmetic composition containing the full starch extract have not been investigated.

Domestic Patent No. 10-1712479, Korean Patent Publication No. 10-2017-0076972

The problem to be solved by the present invention is to provide a cosmetic composition for moisturizing and moisturizing the skin having an anti-inflammatory effect, environmentally friendly and not harmful to the human body without using artificial chemicals, and furthermore a problem of the composition comprising a natural derived extract It is to provide a method for producing a cosmetic composition capable of securing phosphorus storage stability.

In order to solve the above problems, the present invention provides a cosmetic composition comprising 1.5 to 3.0% by weight of the extract of the fern, 0.1 to 1.5% by weight of the polyamine derived from plants, 0.01 to 1.0% by weight of the hinokithiol and the balance of the solvent.

Furthermore, the present invention provides a method of preparing a first composition comprising a green fern extract and a first solvent, preparing a second composition comprising a plant-derived polyamine, hinokithiol, and a second solvent, and the first and second agents. Mixing the composition, the first solvent comprises purified water or glycerin, the second solvent provides a method for producing a cosmetic composition comprising a polyhydric alcohol-based compound.

The cosmetic composition according to the present invention contains an extract of the fern starch is excellent in moisturizing effect. In addition, it has no skin irritation and has anti-inflammatory effect. Furthermore, the solubility, affinity, and stability of various bases, additives, organic solvents, and the like for cosmetic use are good, and storage stability is excellent.

Hereinafter, various embodiments of the present invention are described. It is to be understood that the present invention is not limited to specific embodiments, and includes various Modifications, Equivalents, and / or Alternatives of the embodiments of the present invention. In connection with the description of the drawings, similar reference numerals may be used for similar components.

In this document, expressions such as "have", "may have", "include", or "may contain" include the presence of a corresponding feature (e.g., numerical, functional, operational, or component such as a component). Does not exclude the presence of additional features.

In this document, expressions such as "A or B", "at least one of A or / and B", or "one or more of A or / and B" may include all possible combinations of items listed together. . For example, "A or B", "at least one of A and B", or "at least one of A or B" includes (1) at least one A, (2) at least one B, Or (3) both of cases including at least one A and at least one B.

The expression "Configured to" used in this document is, for example, "Suitable for", "Having the capacity to" depending on the situation. It may be used interchangeably with "," Designed to "," Adapted to "," Made to ", or" Capable of ". The term "configured to" does not necessarily mean "specifically designed to".

The terminology used herein is for the purpose of describing particular embodiments only and may not be intended to limit the scope of other embodiments. Singular expressions may include plural expressions unless the context clearly indicates otherwise. The terms used herein, including technical or scientific terms, may have the same meaning as commonly understood by one of ordinary skill in the art. Among the terms used in this document, terms defined in the general dictionary may be interpreted as having the same or similar meaning as the meaning in the context of the related art, and unless it is clearly defined in this document, in an ideal or excessively formal meaning. Not interpreted. In some cases, even if terms are defined in the specification, they may not be interpreted to exclude embodiments of the present disclosure.

In addition, each step of the process according to the present invention is not limited to that by a complete time series order, but the invention is easily described according to the order applied to the manufacturing process of a general composition, and the process order of the invention is necessary. It is possible to change or modify according to the course, the preparation of the compositions described below may be carried out separately in a separate process.

The embodiments disclosed herein are presented for the purpose of explanation and understanding of the disclosed, technical details, and do not limit the scope of the invention. Therefore, the scope of the present document should be construed as including all changes or various other embodiments based on the technical idea of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail. Prior to this, terms or words used in the present specification and claims should not be construed as being limited to the common or dictionary meanings, and the inventors should properly explain the concept of terms in order to best explain their own invention. Based on the principle that can be defined, it should be interpreted as meaning and concept corresponding to the technical idea of the present invention.

Therefore, the configuration of the embodiments described herein are only some of the most preferred embodiments of the present invention and do not represent all of the technical idea of the present invention, various equivalents and modifications that may be substituted for them at the time of the present application It should be understood that there may be

Throughout the specification, when a part is said to "include" a certain component, it means that it may further include other components, except to exclude other components unless otherwise stated.

Cosmetic composition

The present invention provides a cosmetic composition comprising 1.5 to 5.0% by weight of the extract of the fern, 0.1 to 1.5% by weight of the plant-derived polyamine, 0.01 to 1.0% by weight of the hinokithiol and the remainder of the solvent. The cosmetic composition is particularly useful for moisturizing the skin.

The sesame seed extract is obtained by extracting the active ingredient using a solvent in the sesame seed, filtered.

It belongs to the red algae, its scientific name is Gloiopeltis tenax, reddish brown leathery and awl-shaped. Stems are narrow near the roots, tapering slightly upwards and very pointed. Branched into two branches and bent around. The beads connected with beads like cells are densely gathered to form a cortex, and the threads with many branches are loosely formed in them. A little coarse cylindrical axial cells are connected in length and are bent back and forth in the center of stem. It grows in the lower intertidal zone.

Chamomile fern can be extracted as an active ingredient in the following way, using the following method, it is possible to extract a high concentration of the active ingredient having a variety of efficacy, in particular, to ensure the highest yield through the filtration process can do. When included in the cosmetic composition of the sesame seed extract obtained through this extraction process, the moisturizing effect can be increased.

The present invention uses the dried scabbard, which is sufficiently washed and dried, and then crushed the washed and dried scabbard, and then filtered fine powder using a 300 mesh. Thereafter, the mixture is treated with a solvent at a temperature of 40 to 60 ° C for 12 to 24 hours to obtain an extract. Preferably, the treatment is carried out at a temperature of 50 ° C. for 24 hours.

The solvent used for the extraction is a solvent selected from the group consisting of purified water, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, ethyl ether, butylene glycol and mixtures thereof, more preferably ethanol, 70 Extraction is performed using aqueous ethanol at% concentration or water, most preferably 70% aqueous ethanol.

Ethanol was further added to the final extract of Sesame fern extracted as described above to a final 70% (volume concentration) ethanol aqueous solution, and then refluxed three times for 5 hours, cooled, and filtered through a filter paper. When the filtration is completed in the present invention, the extract obtained in the extraction step is transferred to a concentration tank, concentrated under reduced pressure and dried. Concentration under reduced pressure was concentrated using a rotary vacuum concentrator and concentrated under reduced pressure for 2 hours while putting 700 ml of the extract in a 1,000 ml round flask and rotating at 50 to 70 ° C. and 600 to 1200 rpm at a pressure of 650 mmHg to 750 mmHg. The concentrated solution concentrated under reduced pressure was subjected to prefreezing to lower the temperature to -40 ° C, maintained at -40 ° C for 2 to 4 hours, and then dropped to -80 ° C through trap refrigeration, and the vacuum was maintained at 10 to 30 mTorr. Raise again to -40 ° C over minutes. Thereafter, 240 minutes at -20 ° C, 40 minutes at 0 ° C, 340 minutes at 15 ° C, 130 minutes at 30 ° C and 1300 minutes until complete drying.

According to the present invention, the sesame seed extract is an extract extracted after ultra-high pressure treatment, or the extract is a powder obtained by vacuum, concentrated and frozen or hot air dried, or a combination of the powder and the extraction solvent is preferred.

Chamomile starch extract is preferably included 1.5 to 5.0% by weight, preferably 1.5 to 3.0% by weight relative to the total weight of the cosmetic composition. If the content of the extract is less than 1.5% by weight, the moisturizing effect does not appear, and when the content of the extract is greater than 5.0% by weight, the effect of increasing the content is insignificant, and the stability of the formulation may occur.

The plant-derived polyamine may be any of a low-purity product that has not been purified to improve the polyamine concentration and a high-purity product that greatly increases the polyamine concentration by purifying with an ion exchange resin. The polyamine concentration of low-purity soybean malt germ extract powder is about 0.1-0.2% (percent concentration), but the powder polyamine concentration of high-purity purified soybean embryo extract purified by ion exchange resin is up to 30-50% (percent concentration). It can increase. If the extract is an aqueous solution, it is 1/10 to 1/100 of the concentration.

The said polyamine is a general term of the aliphatic hydrocarbon which has two or more primary amino groups, and is a natural product universally existing in a living body, and 20 or more types of polyamines are discovered. For example, 1,3-diaminopropane, putrescine, cadaverine, carzin, spermidine, homo spermidine, aminopropercaberine, thermin, spermine, thermospermine, cannavar Amine, aminopentyrnorspermidine, N, N-bis (aminopropyl) cardaberine, homospermine, cardpentamine, homocardpentamine, cardo hexamine, homocardo hexamine and the like. . Representative polyamines are putrescine, spermidine, spermine.

Putrescine is one of the representative polyamines, a general natural product commonly present in organisms, and an aliphatic hydrocarbon compound having two primary amino groups. Spermidine is one of the representative polyamines, a general natural product commonly present in living organisms, and an aliphatic hydrocarbon compound having three primary amino groups. Spermine is one of the representative polyamines, a general natural product commonly present in organisms, and an aliphatic hydrocarbon compound having four primary amino groups.

The plant extract including the polyamine and / or the plant extract containing the polyamine as an active ingredient is not particularly limited, but examples thereof include dicotyledonous plants, monocotyledonous plants, herbaceous plants, chimoto plants, gourd plants, Eggplant plants, rice plants, cress plants, ball plants, mallow plants, asteraceae plants, tusks, said plant extracts, said plant extracts, and the like.

There is no restriction | limiting in particular as a plant tissue which obtains the plant extract containing polyamine, and / or the plant extract containing polyamine as an active ingredient. Preferably, it is in seed form, in the process of growth. For plants in the process of growth, the plant extracts of the present invention can be obtained from whole or partial tissues of the plant. The site to be obtained is not particularly limited, but the total number, flower, brain, fruit, fruit, leaf, cotyledon, seed, root, seed, dry seed, embryo and embryo. Preferably, it is a fruit, a leaf, a seed, a seed, a seed, a dry seed, an embryo, an embryo, Especially preferably, it is a seed, a dry seed, an embryo, etc.

The plant-derived polyamine is not limited to a specific range, but is preferably included in 0.1 to 1.5% by weight relative to the total weight of the cosmetic composition.

If the content of the plant-derived polyamine is less than 0.1% by weight does not exhibit a moisturizing effect, when the content of more than 1.5% by weight is insignificant increase in the effect of increasing the content, may cause problems in the formulation stability of the final composition. have.

Hinokitiol, also called β-thujaplicin, is a troplone derivative found in the tree of the Cupressaceae family and is known as a nontoxic safe substance. In functional terms, hinokithiol induces apoptosis through caspase-3 activity and exhibits various biological activities such as anti-inflammatory, antibacterial, anti-tumor and antioxidant activity.

The hinokithiol is β-thujaplicin, 2-hydroxy-4-isopropyl-cyclohepta-2,4,6-trien-1-one (C ₁₀ H ₁₂ O ₂ ) or 4 Also called isopropyltropolone, a compound with a seven-membered ring that is aromatic in its molecule.

The hinokithiol may be prepared by conventional methods known in the art to which the present invention pertains, and may be preferably separated from oils from the leaves, stems, and branches of a cypress, such as a cypress or juniper. This is not restrictive. The cypress (Chamaecyparis obtusa) is a cypress, an evergreen tree of the genus Cypressaceae, which is native to Japan, and is native to Japan in 1904 and is native to Jeju Island and southern regions (Cheng, S., H. Chang). , C. Wu, and S. Chang. 2007. Anti-termitic activities of essential oils from coniferous trees against Coptotermes formosanus.Bioresource technology 98 (2): 456-459).

Β-thujaplicin compound in essential oils obtained from baekbaek is an aromatic tropolone compound with strong anti-inflammatory and antimicrobial effects, and has various physiological activities such as antioxidant and free radical scavenging, food freshness and plant germination inhibition. (Arima et al., 1997; Fukuzawam et al., 1985; Inamori et al., 1991; Koyama et al., 1997; Trust et al., 1973).

As a result of intensive research, the inventors have found that when the hinokithiol is included in a cosmetic composition, it is combined with a plant-derived polyamine to effectively exhibit an anti-inflammatory component that is inherent in the prior art, while the extract of the cosmetic composition of the present invention includes a natural derived extract. Long-term storage stability can be secured.

The hinokithiol is not limited to a specific range, but is preferably included in 0.01 to 1.0% by weight relative to the total weight of the cosmetic composition.

If the content of the hinokithiol is less than 0.01% by weight does not appear anti-inflammatory effect, if the amount is greater than 1.0% by weight increase the effect of increasing the content is minimal, may cause problems in the formulation stability of the final composition.

In the case of the cosmetic composition containing a naturally-derived substance, an opaque appearance and a layer separation phenomenon often occur over time.

However, according to one embodiment of the present invention, by including the plant-derived poly amine and hinokithiol in a weight ratio of 1: 0.1 to 0.5 in the cosmetic composition, it is possible to obtain a transparent cosmetic composition, the effect of the cosmetic composition of the present invention It is possible to secure storage stability without harming.

The solvent may use purified water, ultrapure purified water, which is commonly used in the art, and preferably ultrapure purified water. General purified water is a water generally used in pharmaceuticals, cosmetics, etc., the dissolved solids exhibiting purity of 1.0 to 0.5ppm, the size of the filter used to remove particulates and microorganisms in the preparation of purified water is 0.2 to 0.45㎛, pH is It varies from weak acid to weak alkali. The ultra-pure purified water has a very high purity of 0.03ppm dissolved solids, the size of the filter used in the preparation of ultra-pure purified water is 0.001㎛ removed 99.99% of the microorganisms using this, it is preferable in the cosmetic composition because the pH is neutral. In particular, ultrapure purified water not only prevents automatic oxidation of the composition by removing metal ions, but also prevents protein aggregation due to metal ions, so that the formulation can be applied evenly to the skin while being hypoallergenic.

In addition, from the viewpoint of storage stability, the solvent may use a polyhydric alcohol compound, the polyhydric alcohol compound is specifically hexanediol, ethylene glycol, propylene glycol, dipropylene glycol, tripropylene glycol, diethylene glycol, sorbitol, It may include one or more selected from the group consisting of mannitol, maltitol, lactitol, glucose, polyethylene glycol, butylene glycol and polypropylene glycol, and it is preferable to use butylene glycol for storage stability of the final composition. .

Ingredients included in the cosmetic composition of the present invention as an active ingredient includes components conventionally used in cosmetic compositions in addition to the active ingredient, for example, conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and flavors, And carriers.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art, including, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto.

More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, solvents, solubilizers or emulsions are used as carrier components, such as water, ethanol, isopropanol, ethyl acetate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic esters. , Fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, and microcrystals are used as carrier components. Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

The cosmetic composition of the present invention can be used as a moisturizing cosmetic having excellent skin moisturizing effect.

Manufacturing method of cosmetic composition

Cosmetic composition according to an embodiment of the present invention can be prepared and used in a conventional manner in the art, preferably it may be to use the following manufacturing method.

Specifically, the step of preparing a first composition comprising an extract of the fern and a first solvent, preparing a second composition comprising a plant-derived poly amine, hinokithiol and a second solvent and the first and second compositions It may be to include a step of mixing.

Here, the plant-derived poly amine may include one or more selected from the group consisting of putrescine, spermidine and spermine.

In addition, the plant-derived poly amine and hinokithiol may be mixed in a weight ratio of 1: 0.1 to 0.5.

The first solvent may include purified water or glycerin, and the second solvent may include a polyhydric alcohol compound.

Specific examples of the polyhydric alcohol compound include hexanediol, ethylene glycol, propylene glycol, dipropylene glycol, tripropylene glycol, diethylene glycol, sorbitol, mannitol, maltitol, lactitol, glucose, polyethylene glycol, butylene glycol, and polypropylene glycol. It may include one or more selected from the group consisting of, for the storage stability of the final composition it is preferable to use butylene glycol.

By going through the manufacturing process as described above, it is possible to obtain a transparent cosmetic composition, further minimizing the layer separation phenomenon.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, and the scope of the present invention is not limited by these examples in accordance with the gist of the present invention to those skilled in the art. Will be self-evident.

Preparation Example 1: Preparation of Sesame Seedfish Extract

30 g of fully dried dried lychee starch was finely pulverized to pass through a 300 mesh sieve, and solvent extraction was carried out at a temperature of 50 ° C. for 12 hours to obtain a primary extract of lychee fern. The primary herb extract was maintained at 50 ° C. and subjected to ultra high pressure for 24 hours and then cooled to room temperature. The ultra high pressure wasol extract was extracted by refluxing three times for 5 hours by adding ethanol to the final 70% (volume concentration) ethanol aqueous solution, and then cooled and filtered with Whatman # 2 filter paper. When the filtration is completed in the present invention, the extract obtained in the extraction step was transferred to a concentration tank and concentrated under reduced pressure at 50 ℃ or less and then freeze-dried. The lyophilisate was dissolved in 2% using 50% glycerin to prepare 20 g of the secondary extract of the lychee fern.

The secondary herb extract obtained above was used in Examples and Comparative Examples at the following ratios.

Preparation Example 2 Preparation of Composition

In the case of Examples 1 to 2, 4 to 5 and Comparative Examples, a cosmetic composition was prepared in the composition and content shown in Table 1 below in a single reactor.

Example 3

To prepare a first composition from the cultivars of the cultivars and (purified water) at room temperature in the composition and content shown in Table 1, plant-derived polyamine (mixture of putrescine, spermidine and spermine), hinokithiol and A second composition comprising (hexane) is prepared. Thereafter, the first and second compositions were mixed to prepare a cosmetic composition.

Ingredient (g)
Example 1
Example 2
Example 3
Example 4
Example 5
Purified Water to 100 (g)
Remaining amount
Remaining amount
Remaining amount
Remaining amount
Remaining amount
Chamomile Starch Extract
1.5
3.0
3.0
2.0
2.0
Plant-derived Polyamines
0.5
0.7
0.7
2.0
2.0
Hinoki Thiol
0.1
0.15
0.15
0.1
2.0
Butylene glycol
5.0
5.0
5.0
5.0
5.0
glycerin
3.0
3.0
3.0
3.0
3.0
Fiji-60 Hydrogenated
Castor oil
0.2
0.2
0.2
0.2
0.2
Spices
0.1
0.1
0.1
0.1
0.1

Ingredient (g)
Example 6
Comparative Example 1
Comparative Example 2
Comparative Example 3
Comparative Example 4
Purified Water to 100 (g)
Remaining amount
Remaining amount
Remaining amount
Remaining amount
Remaining amount
Chamomile Starch Extract
5.0
1.0
7.0
3.0
3.0
Plant-derived Polyamines
0.7
0.5
0.5
3.0
0.7
Hinoki Thiol
0.15
0.2
0.15
0.15
1.5
Butylene glycol
5.0
5.0
5.0
5.0
5.0
glycerin
3.0
3.0
3.0
3.0
3.0
Fiji-60 Hydrogenated Castor Oil

0.2

0.2

0.2

0.2

0.2
Spices
0.1
0.1
0.1
0.1
0.1

Experimental Example 1 Induction of Differentiation in Keratinocytes

First, put human keratinocytes to 5,000 cells / well in a 96-well microtiter plate containing KGM (Keratinocyte Growth Media) medium containing 1.0% fetal calf serum. After the fetal calf serum was changed to KGM medium and cultured until 70-80% growth. Human keratinocytes were cultured in a culture flask and grown to about 80%.

Thereafter, 200 μl of the compositions of the Examples and Comparative Examples were treated for 4 days, and then the dissolved protein was removed by mixing a reducing agent such as urea or SDS and beta mercaptomethanol (β-mercaptoethanol). Thereafter, the remaining stratum corneum measured peptide concentration at 310 nm and compared its absorbance values. At this time, the low calcium (0.05 mM) treated group without the extract was used as a negative control group, the high calcium (1.5 mM) treated group without the extract was used as a positive control, the test results are summarized in Table 3 below.

division
Induction effect of keratinocyte differentiation (%)
Negative Control
0
Positive control group
100
Example 1
110
Example 2
121
Example 3
125
Example 4
117
Example 5
115
Example 6
126
Comparative Example 1
105
Comparative Example 2
125
Comparative Example 3
115
Comparative Example 4
118

Experimental Example 2: Increased skin moisturizing effect through human experiment

In order to find out the effect of increasing the skin moisturizing power of the extracts of the locust starfish, we conducted a skin moisture increase effect test as follows and a questionnaire about skin dryness improvement evaluation to the test subjects.

2-1. Skin moisture increase effect

The following formulation is a cosmetic composition prepared in Examples and Comparative Examples, 50 to 20 years old women with poor skin was selected to be applied to the face and the first half twice a day for 1 month. At this time, the skin dryness is measured as the default value by using a moisture meter (corneometer, CM820 courage Khazaka electronic GmbH, Germany) at constant temperature and humidity conditions (24 ℃, 40% humidity) before starting the application. After 2 weeks, 5 weeks, the skin moisture was measured to evaluate the objective effect.

division
Skin moisture growth rate (%)
1 week past
2 weeks past
3 weeks past
Example 1
52
65
75
Example 2
72
81
92
Example 3
75
88
96
Example 4
50
62
72
Example 5
58
66
78
Example 6
65
75
88
Comparative Example 1
40
47
60
Comparative Example 2
61
74
85
Comparative Example 3
42
51
66
Comparative Example 4
48
55
68

Experimental Example 3: Observation of Storage Stability after Preparation

The storage stability of the compositions prepared in Examples and Comparative Examples was evaluated and evaluated. Storage stability was measured by the number of aerobic viable bacteria (bacteria and fungi) according to the bioburden test method of the Ministry of Food and Drug Safety cosmetic safety management standards.

division
Bacterial count (cfu)
Fungal water (cfu)
Example 1
10
Not detected
Example 2
10
Not detected
Example 3
Not detected
Not detected
Example 4
10
10
Example 5
10
10
Example 6
20
30
Comparative Example 1
Not detected
Not detected
Comparative Example 2
40
30
Comparative Example 3
30
50
Comparative Example 4
10
10

Experimental Example 4: Observation of stability over time after preparation

The compositions prepared in Examples and Comparative Examples were placed for at least 12 months at room temperature, at least 1 month at high temperature of 45 ° C., and at least 1 month at low temperature of −4 ° C., and observed stability over time. The results are shown in Tables 7 and 8.

○: Good (no change in appearance, transparent), △: change (appearance is opaque), ×: poor (layer separation occurs)

Test items and
Condition Example 1
Example 2
Example 3
Example 4
Example 5
25 ℃ 12 months ○ ○ ○ △ △ -4 ℃ 1 month ○ ○ ○ △ X 40 ℃ 1 month △ △ ○ △ △

Test items and
Condition Example 6
Comparative Example 1
Comparative Example 2
Comparative Example 3
Comparative Example 4
25 ℃ 12 months △ ○ x x △ -4 ℃ 1 month △ △ △ x x 40 ℃ 1 month △ △ x x x

Claims (7)

Chamomile Starch Extract 1.5-5.0 wt%,
0.1-1.5 wt% of plant derived polyamines,
0.01-1.0 wt% of hinokithiol and
Including the remainder of the solvent,
The plant-derived poly amine and hinokithiol is a cosmetic composition comprising a weight ratio of 1: 0.1 to 0.5 in the composition.
The method according to claim 1,
The content of the sesame seed extract is 1.5 to 3.0 wt% cosmetic composition.
The method according to claim 1,
The plant-derived poly amine is a cosmetic composition comprising one or more selected from the group consisting of putrescine, spermidine and spermine.
delete Preparing a first composition comprising a sesame seed extract and a first solvent,
Preparing a second composition comprising a plant derived poly amine, hinokithiol, and a second solvent, and
Mixing the first and second compositions,
The first solvent comprises purified water or glycerin, the second solvent comprises a polyhydric alcohol compound,
The plant-derived poly amine and hinokithiol is a method of producing a cosmetic composition is contained in a weight ratio of 1: 0.1 to 0.5 in the composition.
The method according to claim 5,
The plant-derived poly amine is a method for producing a cosmetic composition comprising at least one selected from the group consisting of putrescine, spermidine and spermine.
delete