KR101473925B1 - Antiseptic compositions comprising Magnolia grandiflora extract and cosmetic compositions containing the same - Google Patents

Abstract

The present invention relates to an antimicrobial and antidandruff composition containing a perennial plant extract. Specifically, the antimicrobial and antimicrobial composition of the present invention is excellent in antimicrobial and preservative activity by synergistic action of a perennial extract and a caprylyl glyceryl ether compound. And to a method for preparing the same. Wherein the antimicrobial and antidandruff composition comprises 0.1 to 3.0% by weight of a magnolia extract powder, 10 to 40% by weight of purified water heated to 50 to 65 캜, and 10 to 30% by weight of glycerin; Mixing 5 to 40% by weight of at least one glycol selected from the group consisting of butylene glycol, propylene glycol and hexanediol; Mixing 10 to 40% by weight of carpyryl glyceryl ether; And filtering the mixture. The antimicrobial and antidandruff compositions of the present invention can be prepared by a method comprising the steps of:

Description

The present invention relates to an antimicrobial and antidandruff composition containing a perennial plant extract and a cosmetic composition containing the same,

The present invention relates to an antimicrobial and antidandruff composition containing a perennial plant extract, and more particularly, to a composition for antimicrobial and antimicrobial use containing a perennial plant extract, which is excellent in antimicrobial and preservative activity by synergistic action of a perennial extract and a caprylyl glyceryl ether compound, Gt; antimicrobial < / RTI >

In order to increase the shelf life of food or cosmetics, substances with antibacterial or antiseptic properties should be added together. In particular, cosmetics include water, water-soluble components, oils, waxes and lipid-soluble components. Moisture and various organic components of these products act as good nutrients for bacteria and fungi, . Cosmetics contaminated with microorganisms have a risk of skin troubles and microbial infections such as skin rash and inflammation, as well as functional deterioration due to the deterioration of components. Therefore, most cosmetics contain preservatives for the purpose of suppressing or killing microbial growth. Especially, the use period of these preservatives is more important in cosmetic composition because the period of use is very long from several months to several years due to the characteristics of the product.

The basic condition of the preservative to be considered by the characteristics of the above-mentioned cosmetics is that it has a broad antibacterial spectrum, is easy to use and manufacture, and has no skin irritation. In addition, since it is universally usable, it is not affected by the pH of the product, does not deteriorate the effect of the product components, and should be easy to handle. Preservatives commonly used in cosmetics and pharmaceuticals to meet these requirements are paraoxybenzoates and parabens. For the purpose of sterilization, benzalkonium chloride, chlorohexidine gluconic acid, acid and trichlorocarbonylide are used. However, these artificial preservatives cause skin irritation, skin allergy, etc., even with a small amount of use, and the possibility of inducing cancer or gene mutation is emerging. As a result, the need for natural preservatives has emerged for solving the side effects of conventional chemical synthetic preservatives and for the safety of the products.

As an example of such an example, Korean Patent Publication No. 2004-0080717 entitled " Functional Cosmetic Composition Containing Chitosan and Grapefruit Seed Extract with Antiseptic and Antimicrobial Activity ", chitosan and grapefruit seed extract are essential, and grape seed extract, mustard seed extract, A functional cosmetic composition is further disclosed which further comprises an extract selected from Clostridium botulinum extract and Magnoliae extract.

The present inventors have searched various plant extracts having antimicrobial and antiseptic activities. Among them, when studying a method for enhancing the antimicrobial and preservative activity of a selected product of the extract of the magnolius, when used in combination with a specific compound, And an antiseptic activity are increased, thus completing the present invention.

An object of the present invention is to provide a composition for antimicrobial and antidandruff containing antioxidant and antioxidant activity.

Another object of the present invention is to provide a method for producing the composition for antibacterial and antidandruff.

It is still another object of the present invention to provide a cosmetic composition using the antimicrobial and nasal composition containing the black tea extract.

According to the present invention, there is provided an antimicrobial and antidandruff composition comprising a perennial plant extract and carpyryl glyceryl ether. Preferably, the Magnolia plant extract is a hydrothermal extract of Magnoliaceae. It is preferable that the black tea extract and the carpyryl glyceryl ether are mixed at a weight ratio of 0.1 to 3.0: 10 to 40. According to another aspect of the present invention, there is provided a method for preparing a microcrystalline cellulose, which comprises dissolving, in a mixture, 0.1 to 3.0% by weight of magnolia extract powder, 10 to 40% by weight of purified water heated to 50 to 65 캜, and 10 to 30% Mixing 5 to 40% by weight of at least one glycol selected from the group consisting of butylene glycol, propylene glycol and hexanediol; Mixing 10 to 40% by weight of carpyryl glyceryl ether; And filtering the mixture. ≪ IMAGE > At this time, the magnolia seed extract powder is preferably a hydrothermal extract of magnolia. According to the present invention, there is provided a cosmetic composition comprising the antimicrobial and anticorrosion composition in an amount of 0.0001 to 50% by weight based on the total weight of the cosmetic composition, and having excellent preservability.

The cosmetic composition of the present invention can be used in cosmetics such as soft longevity, nutritional lotion, massage cream, nutritional cream, pack, gel, skin stick type cosmetic, lipstick, makeup base, foundation, shampoo, rinse, body cleanser, soap, toothpaste, Ointments, gels, creams, patches or spray formulations.

The antimicrobial and antidandruff compositions of the present invention are safe because they contain natural extracts, and their antimicrobial and preservative activities are greatly increased by the synergistic action of the respective components, so that they are useful for keeping the composition stable for a long period of time when it is used as a preservative for cosmetics.

Hereinafter, the present invention will be described in detail.

Magnolia grandiflora is a plant of Magnoliaceae, native to North America and growing to a height of about 30 meters. It is also known as Yangyoran (洋 玉蘭), hairy in branches and winter eyes. Leaves are alternate long oval or long egg in inverted shape, length 10 ~ 20cm, width 5 ~ 10cm. It is dull and swollen (leather: leather-like texture). The surface is dark green with shiny, with brown hairs on the back and plain edges. The petiole is 2 ~ 3cm long. Flowers bloom in May-June, white, 15 ~ 20cm in diameter, one branch runs on the end of the branch. It has strong aroma, 3 calyxes, and 9-12 petals. There are many pistils and stamens, and the operating table is purple. The fruit is oolongid, ripening in September, oval, short hairs, two red seeds, hanging in red thread. It is native to North America and is planted for ornamental purposes. In the United States, leaves are used for Christmas decorations. Flowers and bark are medicinal herbs are Hwanghae Ran. It is known to be efficacious in treating externalities, headache coloration, dandruff, vomiting, migraine, and hypertension. Korean Patent Laid-Open Publication No. 2009-0020870 discloses that a perennial plant extract can be used for controlling plant diseases.

The present inventors confirmed that the above-mentioned Magnoliaceae extract had antimicrobial and antiseptic activity, and thus, the extract was prepared by various methods. As a result of extracting with various solvents such as water, ethanol, acetone, and chloroform, it was confirmed that each extract had antimicrobial activity against various bacteria. Among them, hydrothermal extract of Respectively. It is preferable that the magnolia berry extract is leaf, stem or a mixed extract thereof of magnolia. In addition, it has been found that the activity is greatly increased when carprylyl glyceryl ether is mixed with the black tea extract at a specific ratio. Accordingly, the present invention utilizes the fact that a composition containing a perennial extract and a carpyryl glyceryl ether is superior in antibacterial and antiseptic activity due to its synergistic action. Preferably, the activity is most excellent when the grape seed extract and caprylyl glyceryl ether are mixed at a weight ratio of 0.1 to 3.0: 10 to 40.

The anticorrosive composition can be prepared by the following method. According to one embodiment of the present invention, 0.1 to 3.0% by weight of the hydrothermal extracted powder is mixed with 10 to 40% by weight of purified water heated to 50 to 65 캜 and 10 to 30% by weight of glycerin. 5 to 40% by weight of glycols such as butylene glycol, propylene glycol, and hexanediol are mixed. 10 to 40% by weight of Carprylyl glyceryl ether is then mixed and stirred. The mixture is prepared by filtration using filter paper.

The antibacterial and antidandruff compositions thus prepared were found to have excellent antibacterial activity against bacteria such as E. coli, S. aureus, P. auruginosa, C. albicans and A. niger. Further, cosmetic compositions containing the antimicrobial and antidandruff composition in an amount of 0.0001 to 50% by weight based on the total composition of the cosmetic composition were prepared and tested for stability, and as a result, it was confirmed that the stability of the composition was excellent. Therefore, the antimicrobial and antidandruff composition containing the perennial extract and Carprylyl glyceryl ether can be usefully used as a preservative in cosmetics. The above-mentioned cosmetics can be used as cosmetics such as softening longevity, nutritional lotion, massage cream, nutritional cream, pack, gel, skin adhesive type, lipstick, makeup base, foundation, shampoo, rinse, body cleanser, soap, toothpaste, , Gels, creams, patches or spray formulations.

[Example]

Hereinafter, the present invention will be described in more detail by way of examples and test examples, but the present invention is not limited by the following examples.

Production Example 1: Production of ethanol extract of magnolia

100g of dried leaves and stem were extracted with 1Kg of 95% EtOH at 65 ℃ for 4 hours. After the extraction process is completed, the filtration process is performed. The filtrate was distilled under reduced pressure to obtain 5.1 g of a magnesia extract of 95% EtOH.

Production Example 2: Preparation of an extract of perennial acetone

Dried leaves of Magnoliaceae and 100g of stem were extracted with 1Kg of Acetone under reflux at 65 ℃ for 4 hours. After the extraction process is completed, the filtration process is performed. The filtrate was distilled under reduced pressure to obtain 6.8 g of Acetone extract powder of Magnoliaceae.

Production Example 3: Production of hot pepper extract of magnolia

Dried leaves and stem of 100g of Magnoliaceae were extracted with 1Kg of purified water at 80 ℃ for 4 hours. After the extraction process is completed, the filtration process is performed. The filtrate was distilled under reduced pressure to obtain 17.8 g of a hydrothermally extracted powder of magnolia.

Production Example 4: Preparation of chloroform-extract of peroxidase

100 g of dried peduncle leaves and stem were extracted with 1 Kg of CHCl ₃ (chloroform) at 65 ° C under refluxing for 4 hours. After the extraction process is completed, the filtration process is performed. The filtrate was distilled under reduced pressure to obtain 9.6 g of a magnolia tree extract CHCl ₃ (chloroform) -extracted powder.

Test Example 1: Antimicrobial activity test (MIC TEST)

The antimicrobial activity of the extract of the perennial plant and the carpyryl glyceryl ether of the above-mentioned production example was confirmed. This was confirmed by the MIC (minimal inhibitory concentration) test, which measures the concentration of the minimum antibiotic (antibacterial agent) that can inhibit bacterial growth.

(Bacteria: 2-4 × 10 ⁷ CFU / mL, fungus: 2-4 × 10 ⁶ CFU / mL) were prepared as shown in Table 1 below. The microtube contains the sample to be tested separately for bacterial and fungal (weighing to determine the concentration to be tested). The samples were vortexed with NB (nutrient broth) for bacteria, PDB (potato dextrose broth) for fungi, and mixed by sonication. At this time, samples that did not dissolve were dissolved in diluted DMSO and diluted with medium. Label in a 96-well plate and add 0.1 ml of the prepared sample to the top of the well (separate for both germ and fungi). Fill the plate with 0.1 mL of the whole 96-well plate, then dilute with 1/2 dilution from the top of the plate. The bottom column was used as a blank with no dilution of the sample and a homogeneous solution. Then, 96 well plate was incubated at 37 ℃.

Bacteria were identified in 18-24hrs, C.albicans in 32-56hrs, A.niger in 36-72hrs, and the absorbance was measured by spectrophotometer.

The results are shown in Table 1 below.

E. Coli S. Aureus P. auruginosa C. Albicans A. Niger Production Example 1 0.5 0.5 One One 0.5 Production Example 2 0.5 One 0.5 One 0.5 Production Example 3 0.25 0.125 0.5 0.5 0.125 Production Example 4 0.5 One One One 0.5 Caprylyl glyceryl ether 0.125 0.032 0.25 0.125 0.125

Among the extracts of the magnolius of the above example, it was confirmed that the hydrothermal extract of Magnolia is the most excellent antimicrobial activity.

Example 1: Preparation of antimicrobial composition containing a black tea extract

1.0 g of the hydrothermal extract of the magnolia prepared in Preparation Example 3 was dissolved while mixing 29 g of purified water heated to 60 ° C and 20 g of glycerin. 1, 2-hexanediol were mixed and 25 g of Carprylyl glyceryl ether was added and mixed. After completion of the mixing, the mixture was filtered through a 1.0 mu m filter paper to prepare an antimicrobial composition.

COMPARATIVE EXAMPLE 1: Preparation of composition for antimicrobial antibacterial agent containing extract of Magnolia

1.0 g of the hydrothermal extracted gentle magnolia powder prepared in Preparation Example 3 was dissolved in 40 g of purified water heated to 60 캜 and 34 g of glycerin while mixing. 1, 2-hexanediol (25 g) were added and mixed. After completion of the mixing, the mixture was filtered through a 1.0 mu m filter paper to prepare an antimicrobial composition.

Comparative Example 2: Preparation of antimicrobial composition containing no extract of Magnolia

25 g of purified water heated to 60 ° C and 25 g of Glycerin were dissolved while mixing the hydrothermal extracted powder produced in Production Example 3. 1, 2-hexanediol were mixed and 25 g of Carprylyl glyceryl ether was added and mixed. After completion of the mixing, the mixture was filtered through a 1.0 mu m filter paper to prepare an antimicrobial composition.

Test Example 2: Antimicrobial activity test (MIC TEST)

The antibacterial activity of the composition of Example 1 was tested in the same manner as in Test Example 1, and the results are shown in Table 2 below.

E. Coli S. Aureus P. auruginosa C. Albicans A. Niger Example 1 0.0625 0.008 0.125 0.0625 0.0625 Comparative Example 1 0.25 0.25 0.25 0.5 0.25 Comparative Example 2 0.25 0.5 One 0.25 0.5

As shown in Table 2, it was confirmed that the composition of Example 1 containing the hydrothermal extract of Magnolia sieboldii and Carprylyl glyceryl ether exhibited more excellent antibacterial activity than the respective antimicrobial activities.

Example 2: Cream production

A cream having the composition shown in Table 3 was prepared by a conventional method.

ingredient (weight%) Example 1 Composition 0.70 glycerin 1.00 Butylene glycol 2.00 Arachidyl alcohol / behenyl alcohol / arachidyl glucoside 2.50 Glyceryl stearate 0.50 FAGE-4 olivate 0.50 Caprylic / capric triglyceride 5.00 Cholesteryl / behenyl / octyldodecyllaurylglutamate 1.00 Almond oil 1.00 Dimethicone 0.50 Cyclopentasiloxane / cyclohexasiloxane 2.00 Cetearyl alcohol 1.00 Mineral oil 2.50 Disodium iodide 0.03 BH 0.05 Tocopheryl acetate 0.30 Panthenol 0.20 To a solution of < RTI ID = 0.0 & 0.20 Propylparaben 0.10 Methylparaben 0.20 incense 0.01 Purified water Balance

Test Example 3: CHALLENGE TEST

The flushing force of the composition of Example 1 was evaluated as follows in order to confirm the number of viable bacteria remaining after inoculating a certain number of bacteria into the cream of Example 2 as follows.

Preparation of Test Bacteria

Three bacteria such as Escherichia coli ATCC 8739, Staphlyococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 9027 were inoculated into soybean casein digest agar medium, respectively, and Candida albicans ATCC 10231, Is inoculated into Sabouraud glucose liquid medium. Four kinds of test bacteria inoculated in liquid medium are cultured for 18 hours in shaking incubator at 30 ~ 35 ℃ and used as test bacteria. In addition, Aspergillus niger ATCC 16404 is used as a test organism by collecting spores using sterilized physiological saline in a cultured plate medium.

Inoculation of the test bacteria and measurement of the number of bacteria

Samples were weighed 20 g each in a clean container and then weighed in an amount of 20 g each of Escherichia coli ATCC 8739, Staphlyococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, Aspergillus niger ATCC 16404, Add 200 μl of each of the cultures of 5 kinds of Candida albicans (ATCC 10231) to 20g of the sample, mix well with sterilized Spetula, and mix well. Bacteria are mixed at an initial concentration of 10 ⁶ cfu / g per sample, and fungi are mixed at an initial concentration of 10 ⁵ cfu / g per sample. The samples inoculated with the test bacteria are cultured for 4 weeks in an incubator at 25 ° C., and 1 g of each sample is taken on days 1, 7, 14, 21 and 28, and the viable cell count is measured. Bacteria were cultured for 24 to 48 hours at 30 ~ 35 ℃ in incubator, for 3 to 5 days at 25 ℃ in incubator, and detected on plate medium. The number of viable cells is measured.

Buoyancy determination

The criteria for buoyancy assessment were CTFA standard. After 7 days, more than 99.9% of inoculated bacteria were killed and no more than 4 weeks of bacterial proliferation. After 7 days, more than 90% of inoculated bacteria were killed and more than 4 weeks There should be no bacterial growth.

The results are shown in Table 4 below.

Strains 0 1 week 2 weeks 3 weeks 4 weeks CFU / g %
reduction result %
reduction result %
reduction result %
reduction result E. coli 1.00E + 06 99.99% Pass 99.99% Pass 99.99% Pass 99.99% Pass S.aureus 1.00E + 06 99.99% Pass 99.99% Pass 99.99% Pass 99.99% Pass P.aeruginosa 1.00E + 06 99.99% Pass 99.99% Pass 99.99% Pass 99.99% Pass C.albicans 1.00E + 05 99.99% Pass 99.99% Pass 99.99% Pass 99.99% Pass A. niger 1.00E + 05 99.99% Pass 99.99% Pass 99.99% Pass 99.99% Pass

As a result of buoyancy test, the bacteria showed more than 99.9% death after 7 days of inoculation of the test organism, no further bacterial growth until 28th day, and more than 90% of dead organisms after 7 days of inoculation, And it was confirmed that the bacterium maintains buoyancy in both bacteria and fungi.

Test Example 4: Stability test

The compositions of Examples 1 and 2 were observed in a temperature cycling chamber (4 ° C ↔45 ° C, every 24 hours), and the results are shown in Table 5 below.

Example 1 Example 2 Early - - 1 week - - 2 weeks - - 4 weeks - - 8 weeks - - 12 weeks - - 16 weeks - -

(-; no change, +; slight color change, ++; color change or slight precipitation, +++; color change and precipitation)

As shown in Table 5, the compositions of Example 1 and Example 2 did not show any change in properties in the property stabilization test, and it was confirmed that the composition stability was high.

Claims (7)

An antimicrobial and antidandruff composition comprising a perennial plant extract and caprylyl glyceryl ether. The antimicrobial and antidandruff composition according to claim 1, wherein the perennial plant extract is a hydrothermal extract of perennial plant. The antimicrobial and antidandruff composition according to claim 1, wherein the sacrose extract and caprylyl glycyl ether are mixed at a weight ratio of 0.1 to 3.0: 10 to 40. 0.1 to 3.0 wt% of magnolia extract powder, 10 to 40 wt% of purified water heated to 50 to 65 캜, and 10 to 30 wt% of glycerin are mixed and dissolved; Mixing 5 to 40% by weight of at least one glycol selected from the group consisting of butylene glycol, propylene glycol and hexanediol; Mixing 10 to 40% by weight of carpyryl glyceryl ether; And filtering the mixture. ≪ RTI ID = 0.0 > 11. < / RTI > The method for producing an antimicrobial and antimicrobial composition according to claim 4, wherein the magnolia extract powder is a hot-water extract of magnolia. A cosmetic composition comprising the antibacterial and antidandruff composition according to any one of claims 1 to 3 in an amount of 0.0001 to 50% by weight based on the total weight of the cosmetic composition. The cosmetic composition according to claim 6, wherein the cosmetic composition is at least one selected from the group consisting of softening agents, nutritional lotion, massage cream, nutritional cream, pack, gel, skin adhesive type cosmetic, lipstick, makeup base, foundation, shampoo, rinse, Soap, toothpaste, mouthwash, lotion, ointment, gel, cream, patch or spray agent.