KR102163969B1 - Cosmetic Compositions Containing Fermented Extracts of Vernicia fordii - Google Patents

Abstract

The present invention relates to an antioxidant, anti-wrinkle, whitening, anti-inflammatory and antibacterial cosmetic composition comprising a fermented extract ( Vernicia fordii ) as an active ingredient.
The flow ( Vernicia fordii ) fermented extract provided from the present invention has 6 to 7 times increased flavonoid content compared to the flow ( Vernicia fordii ) extract before fermentation, providing excellent antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial effects. Therefore, it can be used as a cosmetic composition for antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial.

Description

Cosmetic Compositions Containing Fermented Extracts of Vernicia fordii}

The present invention relates to an antioxidant, anti-wrinkle, whitening, anti-inflammatory and antibacterial cosmetic composition comprising a fermented extract ( Vernicia fordii ) as an active ingredient.

Vernicia fordii is a deciduous arboreous tree of the dicotyledonous plant of the Euphorbia family, and is commonly referred to as oil paulownia tree because oil comes from tree seeds. It is native to China, and it is also grown on the southern coast of Korea. The height of the tree grows to about 7.5~10 m, and the bark is grayish brown, with blood trees, and thick branches spread in all directions. Leaves grow alternately and have a heart shape or round shape, and flowers bloom reddish white in May. The fruit is a capsule and matures in September, and it is round and contains three seeds. In the southern regions, the flow is planted as green or garden water, and oil is collected from seeds, and this oil is called "dongyu" (桐油). Dongyu has traditionally been used to light a fire in the East, and it is also important for industrial purposes in modern times.

The fruit of the fluid is effective in traditional oriental medicine such as tongtopungdam and small seed poisoning, and has been used to treat pungdam posterior (occipital tuberculosis, laryngeal syphilis, etc.), improvement, alone, burns, pustular sores, food redness, and fecal discomfort. The root has the effect of news, sputum, hwadam, and insecticide, and has been used to treat dietary obesity, tree species, gochang, hyocheon, nahyeok, and roundworm disease. The leaves have the effect of small species and detoxification, so they were used for the treatment of ongchang, single, gyeomchang, alumni, improvement, and dysentery.

The flow contains flavonoids, which are powerful antioxidants, and through chemical composition studies, diterpenoid esters, sterols, tannins, oil, coumarin and couma Research results are known to contain coumarolignan compounds. As for physiological activity studies on flow, human polymorphonuclear leukocyte activating factor, human neutrophil-activating factor, etc. were found, and extracts of flow and diterpene esters were found. It has been reported that Epstein-Barr virus activity is induced by this. In addition, the flow of 12-O-hexadecanoyl-16-hydroxyphorbol 13-acetate is a human T-lymphotrophic virus type I (Human T-lymphotrophic virus). type I, HTLV-I) induced lymphocyte colony formation.

As a technology using the oil ( Vernicia fordii ) extract, in the case of Korea, Korean Patent Application Publication No. 10-2013-0135133 ``Prevention of skin aging containing oil extract, a fraction thereof, or a diterpene compound isolated therefrom as an active ingredient, and Pharmaceutical composition for improvement”, Korean Patent Publication No. 10-1331148, “Antiviral composition containing a liquid extract or a fraction thereof as an active ingredient”, and the like are disclosed.

On the other hand, Hwangbaek ( Phellodendron amurense ) refers to a medicinal material manufactured by drying the bark of a deciduous tree of Hwanggyeong-pi , a deciduous tree belonging to the Sanchodae , or a medicinal material such as Hwanggyeong-pi , Jabyeok , Sobyeok, and Danhwan . The stem bark is light gray, but the cork layer is developed to crack deeply, and the cork layer is peeled off the inner skin is yellow. The part mainly used as a medicinal material is bark, and generally has a thickness of about 3mm and a length of about 30cm. In addition, Hwangbyeokgeun, which is the root of Hwangbyeok tree (Hwangbaek tree) called Danhwan, is known to have the effect of curing diseases in Myeongchi. It has a peculiar smell, is mucous, and colors saliva yellow. The medicinal effect is similar to the golden or yellow lotus, but there is a difference in the area where the medicinal effect is applied. Known pharmacological actions include antimicrobial action, astringent action, subcutaneous bleeding absorption promotion action, anti-inflammatory action, health stomach action, blood pressure lowering action, muscle contraction enhancement action, etc. It is used for fever, detoxification, diarrhea, diabetes, jaundice, obesity, joint pain, and muscle pain.

Hwangchil ( Dendropanax mordifera ) is an evergreen tree that grows in the forests of the southern beaches and islands of Korea. Yellowish liquid comes out when the skin is scratched. Leaves are alternate, 3-5 branches, but in old trees, the leaves are ovate, oval, pointed at the end, and 10-20cm long. There are no hairs on both sides, and there are petioles. The flowers are bisexual, hangs in an umbel at the end of the branch, the length of the flower shaft is 3-5cm, there are nectar, and the peduncle is 5-10mm long. The calyx is bell-shaped, has 5 ends, 5 petals, 5 stamens, 5 ovaries, and 5 pistils. Fruits are drupe, oval and black. The sap is used for painting furniture and is planted for ornamental purposes. Hwangchil is mainly composed of essential oils, and is a pale yellow, rich oily liquid with a refreshing scent and bitter taste. The essential oil contained in Hwangchil is mainly high-bulk, its main component is Sesqui-terpene, and other alcohols and esters are contained. Hwangchil has anticancer action, antibacterial action, antioxidant action, blood purification action, pain relief action, and nerve stabilization action, strengthening immunity, regenerating hard tissues (bones and teeth), improving liver function, sprains, benthic scent, dyspnea, half body incompetence, blood It has excellent efficacy in promoting circulation, stroke, migraine, menstrual impurity, arthritis, and limb pain.

The present inventors of the present inventors leuconostoc mesenteroides isolated from ginseng ( Leuconostoc mesenteroides ) GFC 160704 strain (accession number: KCTC18474P) fermented flow ( Vernicia fordii ) fermentation extract before fermentation flow ( Vernicia fordii ) extract compared to flavonoids (Flavonoid) The present invention was completed by discovering that the content was increased by 6 to 7 times to provide excellent antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial effects.

Republic of Korea Patent Publication No. 10-2013-0135133 Korean Patent Publication No. 10-1331148

An object of the present invention is to provide a cosmetic composition for antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial cosmetic composition comprising a fermented strain ( Vernicia fordii ) fermented extract as an active ingredient.

Another object of the present invention is to provide a cosmetic comprising the antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial cosmetic composition.

In order to achieve the above object, the present invention provides an antioxidant, anti-wrinkle, whitening, anti-inflammatory and antibacterial cosmetic composition comprising a fermented extract ( Vernicia fordii ) as an active ingredient.

In the present invention, the fluid ( Vernicia fordii ) fermented extract is obtained by inoculating and fermenting the Leuconostoc mesenteroides GFC 160704 strain (accession number: KCTC18474P) in the fluid ( Vernicia fordii ) extract. do.

In the present invention, the cosmetic composition is characterized in that it further comprises a Hwangbaek ( Phellodendron amurense Rupr ) extract and Hwangchil ( Dendropanax mordifera ) extract.

In the present invention, the ratio of the fluid ( Vernicia fordii ) fermented extract, the Hwangbaek ( Phellodendron amurense Rupr ) extract and the hwangchil ( Dendropanax mordifera ) extract is characterized in that the weight ratio is 1:0.01~0.5:0.01~0.5.

In the present invention, the flow ( Vernicia fordii ) fermented extract is characterized in that it contains 0.03 to 20% by weight based on the total weight of the cosmetic composition.

In the present invention, the flow ( Vernicia fordii ) fermented extract is characterized in that the content of flavonoids is increased 6 to 7 times compared to the flow ( Vernicia fordii ) extract before fermentation.

In the present invention, the cosmetic composition is a skin ointment, cream, soft lotion, nutrient lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, Astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, eye cream, moisture cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and It is characterized in that it is any one formulation selected from the group consisting of a body cleanser.

In addition, the present invention provides a cosmetic comprising the cosmetic composition.

The flow ( Vernicia fordii ) fermented extract provided from the present invention has 6 to 7 times increased flavonoid content compared to the flow ( Vernicia fordii ) extract before fermentation, providing excellent antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial effects. Therefore, it can be used as a cosmetic composition for antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial.

1 is a graph showing the evaluation of cytotoxicity.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by an expert skilled in the art to which the present invention belongs. In general, the nomenclature used in this specification is well known and commonly used in the art.

Throughout the specification of the present application, when a certain part "includes" a certain component, it means that other components may be further included rather than excluding other components unless otherwise stated.

According to one embodiment of the present invention, it relates to a cosmetic composition for antioxidant, wrinkle improvement, whitening, anti-inflammatory, and antibacterial, comprising a fermented extract of Yuyu ( Vernicia fordii ) as an active ingredient.

The flow fermentation extract is obtained by inoculating and fermenting the Leuconostoc mesenteroides GFC 160704 strain (accession number: KCTC18474P) (hereinafter abbreviated as'GFC 160704') to the flow ( Vernicia fordii ) extract. To do.

The flow extract is preferably extracted using one or more of the leaves, stems, and bark of the flow, but among them, the use of the leaf portion is more preferable in terms of remarkably increasing the polysaccharide content during fermentation extraction. In the case of using the flow, the leaves, stems, and bark of the flow tree can be crushed or shredded to a size of 10 to 500 mesh.

In one embodiment, the flow fermentation extract is prepared by adding 100 to 2000 parts by weight of an extraction solvent with respect to 100 parts by weight of the flow raw material, and then inoculating the GFC 160704 strain, culturing and fermenting, filtration and extraction. Can be obtained.

As the extraction solvent, a conventional extraction solvent may be used. As the extraction solvent, water, acetone, ethyl acetate, butyl acetate, diethyl ether, benzene, chloroform, hexane, and alcohol having 1 to 10 carbon atoms or a mixed solvent thereof are used as the extraction solvent to be prepared using the following extraction method. I can.

In one embodiment, the alcohols having 1 to 10 carbon atoms include lower monohydric alcohols such as methanol, ethanol, butanol, pentanol, and hexanol, 1,2-pentanediol, 1,5-pentanediol, hexanediol, heptanediol , Intermediate dihydric alcohols such as octanediol and decanediol, and lower trihydric alcohols such as propylene glycol, 1,3-butylene glycol, and glycerin may be used. These may be used alone or in combination of two or more.

In another embodiment, the flow fermentation extract may use a solution capable of strain cultivation as an extraction solvent, and a fermentation extract may be obtained by adding flow to the extraction solvent, inoculating the GFC 160704 strain, and culturing it.

According to one embodiment of the present invention, after inoculating the GFC 160704 strain into the flow extract, it is preferable to incubate for 3 to 7 days at 27 to 37°C when fermenting. If the cultivation temperature and time are out of the above range, it is not preferable because the cultivation conditions of the fermentation strain do not match and the strain does not grow. In addition, flavonoid components contained in the flow fermentation extract may not increase, and there may be problems in that sufficient antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial effects do not appear.

The GFC 160704 strain may be used as a seed culture in order to induce the activity of the strain. For example, when culturing the GFC 160704 strain, a medium is prepared by adding 1 to 2 parts by weight of flow to 100 parts by weight of the MRS medium, and inoculating the GFC 160704 strain in the medium to which the flow has been added to inoculate the GFC 160704. It is preferable because it is effective in generating polysaccharides by easily inducing the activity of the strain. The seed culture is preferably cultured at 27 to 37° C. for 3 to 7 days in terms of remarkably enhancing the polysaccharide content. The cultured seed can be directly inoculated into the flow to produce a fermented product.

In addition, in order to smooth the growth of the strain and increase the enzyme activity, lactose may be additionally added and fermented to prepare a fermented product. In this case, the lactose is preferably contained in an amount of 0.5 to 50 parts by weight based on 100 parts by weight of the flow extract in terms of the most smooth growth of the GFC 160704 strain and optimal increase in enzyme activity.

According to an embodiment of the present invention, the flow fermentation extract obtained from the above method may be included in an amount of 0.03 to 20% by weight, more preferably 5.0 to 10.0% by weight, based on the total weight of the cosmetic composition. When the liquid fermented extract is less than 0.03% by weight based on the total weight of the cosmetic composition, the function as a skin cosmetic is deteriorated because it cannot sufficiently obtain antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial effects, and when it exceeds 20% by weight It is uneconomical because it can exhibit sufficient efficacy even if it does not contain more than that.

The flow fermentation extract may also include an extract that has undergone a conventional purification process. For example, through various additional purification methods, such as separation using an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity, or affinity). The obtained fraction may also be included in the extract of the present invention. In addition, the flow fermentation extract of the present invention may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

In one embodiment, the flow fermentation extract is preferably produced through centrifugation, sterilization, and filtration in terms of preventing destruction of active ingredients by propagation of microorganisms in the future and securing product stability.

The flow fermentation extract is characterized in that the content of flavonoids is increased 6 to 7 times compared to the flow extract before fermentation. In other words, the flavonoid component contained in the flow extract is increased compared to before fermentation through the fermentation process by the GFC 160704 strain, so not only skin beauty, such as antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial, etc., but also fundamental skin health There is an effect that can implement improvement.

The cosmetic composition is preferable in terms of further increasing the flavonoid content and maximizing the efficacy as a cosmetic to further include a Hwangbaek ( Phellodendron amurense Rupr ) extract and a Hwangchil ( Dendropanax mordifera ) extract.

Hwangbaek and Hwangchil used to prepare the Hwangbaek extract and Hwangchil extract are not particularly limited in varieties and sites. The hwangbaek and hwangchil may be crushed or shredded to a size of 10 to 500 mesh.

Hwangbaek extract and hwangchil extract according to the present invention can be obtained through various extraction methods known in the field to which the present invention belongs, specifically water, acetone, ethyl acetate, butyl acetate, diethyl ether, benzene, chloroform, hexane And an alcohol having 1 to 10 carbon atoms or a mixed solvent thereof as an extraction solvent, and may be prepared by using the following extraction method. The solvent is used as an extraction solvent, and when such an extraction solvent is used, the content of the extracted active ingredient is increased, so that antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial effects can be further improved.

In one embodiment, the alcohol having 1 to 10 carbon atoms is a lower monohydric alcohol such as methanol, ethanol, butanol, pentanol, and hexanol, 1,2-pentanediol, 1,5-pentanediol, hexanediol, heptanediol , Intermediate dihydric alcohols such as octanediol, decanediol, propylene glycol, 1,3-butylene glycol, and lower trihydric alcohols such as glycerin may be used. These may be used alone or in combination of two or more.

In one embodiment, the extraction method of the Hwangbaek extract and Hwangchil extract is not particularly limited, but, for example, during stirring extraction, reflux cooling extraction, cold needle extraction, ultrasonic extraction and supercritical extraction by immersing Hwangbaek or Hwangchil in an extraction solvent It may include preparing an extract by applying one or more extraction methods.

The ratio of the complex extract in which the flow fermentation extract, Hwangbaek extract, and Hwangchil extract are mixed is included in a weight ratio of 1:0.01 to 0.5:0.01 to 0.5, which can increase the extraction efficiency and further increase the flavonoid content. It is preferable in that there is.

In one embodiment, the flow fermentation extract, hwangbaek extract and hwangchil extract are mixed in an optimal ratio to prepare a complex extract, and then obtained through a concentration process.

In one embodiment, the concentration may be carried out using an evaporative concentration or reduced pressure concentration method. More specifically, the fluidized fermentation extract, Hwangbaek extract, and Hwangchil extract may be mixed, and then added to a rotary vacuum evaporator for concentration.

As described above, the flow fermented extract fermented with the GFC 160704 strain has excellent antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial effects, has no cytotoxicity, is harmless to the human body, and can be used as a cosmetic composition with little skin irritation. .

The cosmetic composition for antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial of the present invention includes skin ointment, cream, softening lotion, nutrient lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin Lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, eye cream, moisture cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing It may have any one formulation selected from the group consisting of lotion, cleansing cream, body lotion, and body cleanser, but is not limited thereto. The composition of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the present invention is a paste, cream, or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc acid may be used as a carrier component. I can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, a liquid diluent such as water, ethanol or propylene glycol as a carrier component, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

When the formulation of the present invention is a surfactant containing cleansing, as a carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linoline derivatives, or ethoxylated glycerol fatty acid esters may be used.

In addition, the cosmetic composition may further include a base component that can be blended in a conventional cosmetic composition. In specific examples, solubilizers, surfactants, moisturizing agents, thickeners, pH adjusters, preservatives, antioxidants, sequestering agents, bactericides, anti-inflammatory agents, antimicrobial agents, solubilizing agents or flavoring agents, dyes, pigments, etc. may further be included. .

Surfactants that may be included in the composition include anionic surfactants such as ammonium lauryl sulfosuccinate and ammonium lauryl sulfate, and nonionic surfactants such as polyoxyethylene alkyl ether and polyoxyethylene fatty acid ester. In addition, cationic surfactants such as tertiary aliphatic amine salts, alkyltrimethylammonium chloride, and the like, and amphoteric surfactants such as betaine and amide betaine type may be used.

The moisturizing agent may be sodium hyaluronate, glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, sorbitol, etc., and the thickener may be xanthan gum, methylcellulose, hydroxyethylcellulose, carrageenan, carboxy It may be a water-soluble polymer compound such as methylcellulose and hydroxymethylcellulose. In addition, the pH adjuster may specifically include citric acid, sodium hydroxide, triethanolamine, sodium citrate, phosphoric acid, sodium phosphate, and lactic acid.

The preservative may be benzoic acid, paraoxybenzoic acid ester, a mixture of methylchloroisothiazolinone, phenoxyethanol, DMDM hydantoin, and the like, and the antioxidant may be specifically dibutylhydroxytoluene, ascorbic acid, etc. .

The sequestering agent may be ethylenediamine tetraacetate disodium, ethylenediamine tetraacetate tetrasodium, and the like, and the disinfectant may be specifically chlorhexidine gluconate, quaternary ammonium salt, pyroctoneolamine, zinc pyrithione suspension, iodopropynyl butyl It can be carbamate, salicylic acid, etc.

The anti-inflammatory agent may include monoammonium glycyrrhizate, dipotassium glycyrrhizin, allantoin, and mixtures thereof, and antimicrobial (antimicrobial) components are specifically phenoxyethanol, chlorhexidine, chlorhexidine gluconate, benzoic acid and salts thereof, benzyl alcohol, salicylic acid. , Triclocarban, zinc pyrithione suspensions, and mixtures thereof.

Specifically, the solubilizing agent may be PG 60-hydrogenated castor oil, and the flavoring agent may use conventional bases used in the cosmetic composition field of the present invention.

Since the cosmetic composition obtained from the above method provides excellent antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial effects, it is possible to provide cosmetics that are effective for skin improvement.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for describing the present invention in more detail, and that the scope of the present invention is not limited by these examples according to the gist of the present invention, for those of ordinary skill in the art to which the present invention belongs. It will be self-evident.

<Example 1> Preparation of flow fermentation extract

The flow was used by purchasing a completely dried domestic product from Jecheon Herbal Medicine. First, in order to make fermentation by the strain more efficient, 100 g of MRS medium was added to the leaves. 2 g of powder (200 mesh) was added, and then Leuconostoc mesenteroides GFC 160704 strain (accession number: KCTC18474P) isolated from ginseng was inoculated with 1.0 X 10 ⁷ CFU to the medium, and at 37°C. Incubated with stirring for 3 days to incubate the seed.

Next, 20 kg of 50% ethanol was added to 1 kg of dried flowing leaf powder (200 mesh), sterilized at 85° C. for 60 minutes, stirred at room temperature for 3 days, extracted by repeating the filtration process 3 times, and then extracted by rotating vacuum It was concentrated in a concentrator (Eyela, Japan) to prepare a flowing extract.

The seed cultured Leuconostoc mesenteroides GFC 160704 strain (accession number: KCTC18474P) was inoculated with 10 g of the prepared flow extract with 1.0 X 10 ⁷ CFU. At this time, 5 g of lactose was added to facilitate the growth of the strain and increase the enzyme activity, followed by culturing at 37° C. for 7 days to ferment to prepare a fermented product. The fermented product was centrifuged at 8000 rpm for 20 minutes to remove the cells, followed by filtration and sterilization to prepare a final flow fermentation extract.

<Example 2> Preparation of complex extract

Hwangbaek and Hwangchil were purchased and used completely dried from Jecheon Herbal Medicine. Hwangbaek was prepared by cutting the stem and root parts into 50 mesh size, and Hwangchil was prepared by cutting leaves and stem parts into 50 mesh size.

Add 20 kg of 50% ethanol to each 1 kg of Hwangbaek and Hwangchil, sterilize at 85°C for 60 minutes, then extract by stirring and extracting for 3 days at room temperature, followed by extraction by repeating the process of filtering 3 times, and then a rotary vacuum concentrator (Eyela, Japan) to prepare Hwangbaek extract and Hwangchil extract.

Next, 10 g of the flow fermentation extract obtained in Example 1, 0.5 g of Hwangbaek extract, and 0.2 g of Hwangchil extract were mixed to prepare a complex extract.

<Comparative Example 1> Preparation of flowing extract

The flow was used by purchasing a completely dried domestic product from Jecheon Herbal Medicine. After adding 20 kg of 50% ethanol to 1 kg of dried floating leaf powder (200 mesh), sterilizing at 85°C for 60 minutes, stirring extraction at room temperature for 3 days, extracting by repeating the filtration process 3 times, and then in a rotary vacuum concentrator. Concentrated to prepare a flowing extract.

<Comparative Example 2> Preparation of Hwangbaek and Hwangchil complex extract

Hwangbaek and Hwangchil were purchased and used completely dried from Jecheon Herbal Medicine. Hwangbaek was prepared by cutting the stem and root parts into 50 mesh size, and Hwangchil was prepared by cutting the leaves and stem parts into 50 mesh size.

Add 20 kg of 50% ethanol to each 1 kg of Hwangbaek and Hwangchil, sterilize at 85°C for 60 minutes, extract by stirring at room temperature for 3 days, and then extract by repeating the process of filtering 3 times, and then concentrated in a rotary vacuum concentrator. Hwangbaek extract and hwangchil extract were prepared.

Next, a composite extract was prepared by mixing 1 g of Hwangbaek extract obtained in Example 1 and 0.3 g of Hwangchil extract.

<Experimental Example 1> Flavonoid content analysis

The extracts of Examples 1 to 2 and Comparative Examples 1 to 2 were each immersed in 80% or more of ethanol and precipitated at 4° C. for 24 hours, followed by centrifugation for 20 minutes to separate the precipitate. After dissolving the separated precipitate by adding 5% distilled water, it was heated at 105°C to distill ethanol, and the filtrate obtained by filtration with an ultrafiltration membrane was lyophilized in a freeze dryer (Eyela FDU 540, Japan) to separate the flavonoids. The content was measured and the results are shown in Table 1 below.

division Flavonoid content (mg/g) Example 1 32.30.58 Example 2 48.50.18 Comparative Example 1 5.010.22 Comparative Example 2 2.10.19

As a result, as shown in Table 1, it was confirmed that in Examples 1 to 2, the content of flavonoids was significantly increased compared to Comparative Examples 1 and 2.

In particular, the flow fermentation extract of Example 1 had a flavonoid content of 32.3 mg/g, and the flow extract of Comparative Example 1 had a flavonoid content of 5.01 mg/g, and flavonoids ( Flavonoid) content increased 6-7 times. It was confirmed that the lactic acid bacteria Leuconostoc mesenteroides GFC 160704 strain (accession number: KCTC18474P) isolated from ginseng increased the content of flavonoids.

In addition, among Examples 1 to 2, the flavonoid content of Example 2 was increased by 16.2 mg/g compared to Example 1, and the flow fermentation extract, Hwangbaek extract, and Hwangchil extract were mixed compared to when the flow fermentation extract was used alone. It was confirmed that the flavonoid content was further increased when one complex extract was used.

<Experimental Example 2> Cytotoxicity test: WST (Water-soluble tetrazolium salt) assay

Cytotoxicity experiments were performed on RAW 264.7 cells in order to confirm whether the extracts of Examples 1 to 2 and Comparative Examples 1 to 2 have irritation to cells.

RAW 264.7 cells, a type of macrophage, were equally counted and dispensed into 24 well plates at 1.0x10 ⁴ cells/well using DMEM medium containing 1% penicillin/scraptomycin and 10% Fetal bovineserum (FBS). Incubated for 24 hours at 37°C and 5% carbon dioxide. Examples 1 to 2 and Comparative Examples 1 to 2 were mixed with the medium at each concentration (0, 10, 25, 50, 100㎍/mL) to RAW 264.7 cells cultured for 24 hours, and then 1 mL was added to each well. And reacted for 24 hours in an incubator under conditions of 37°C and 5% CO ₂ , and after taking only the supernatant of each well separately, add WST-1 assay solution (ez-cytox) to each well and react for 2 hours in an incubator. The absorbance was measured at 450 nm with a reader.

Cell viability was calculated using Equation 1 below in comparison with the treated group (untreated group) not treated with the sample.

[Equation 1]

Cell viability (%) = (absorbance of sample treated group / absorbance of untreated group) * 100

As a result, as shown in FIG. 1, it was confirmed that all of the RAW 264.7 cells did not show cytotoxicity, and thus, can be used as a safe material for cosmetics even after fermentation.

<Experimental Example 3> Antioxidation test: DPPH radical scavenging activity

Antioxidant efficacy tests were conducted using the extracts of Examples 1 to 2 and Comparative Examples 1 to 2.

Free radical refers to free radicals, and the oxygen we breathe creates energy and is a substance with a high oxidizing power that is thousands of times higher in the process of being reduced to water. It is produced in the metabolic process of cells in the body of animals and plants, or it is generated by stress, photostimulation, and bacterial penetration, and when an excessive amount of active oxygen is present in the body, it indiscriminately attacks normal cells and becomes the main cause of various diseases and aging. In addition, it acts as a pathological factor showing an immune response sensitive to external stimuli. Whether the extract of the present invention exhibits antioxidant activity and thus has an inflammation alleviating effect was confirmed by the free radical scavenging ability of DPPH, and the extracts of Examples 1 to 2 and Comparative Examples 1 to 2 were diluted to a concentration of 50 (㎍ / ㎖), respectively. The degree of inhibition of DPPH was evaluated. After diluting the extracts of Examples 1 to 2 and Comparative Examples 1 to 2 to a concentration of 50 (µg/ml), respectively, after mixing 250µl of 0.1M DPPH (1,1-diphenyl-2-picrylhydrazyl) solution for 30 minutes It was reacted at 4°C. When the reaction was over, each sample was placed in a 96 well plate and absorbance was measured at 520 nm using an ELISA reader. At this time, ascorbic acid was used as a positive control.

In order to confirm the DPPH radical scavenging activity, it was calculated using Equation 2 below.

[Equation 2]

Free radical scavenging ability (%) = [100-(absorbance of the sample treated group/absorbance of the untreated group X 100)]

division Free radical scavenging activity (%) Positive control 70.321.31 Example 1 69.11.04 Example 2 88.10.54 Comparative Example 1 68.40.13 Comparative Example 2 62.30.44

As a result, as shown in Table 2, it was confirmed that Examples 1 to 2 had superior DPPH radical scavenging ability compared to Comparative Examples 1 and 2. In particular, it was confirmed that the DPPH radical scavenging ability was significantly increased in Example 2 than in Example 1 among Examples 1 to 2, and the flow fermentation extract, Hwangbaek extract, and Hwangchil extract were mixed compared to when the flow fermentation extract was used alone. When the complex extract was used, it was confirmed that the antioxidant activity was more excellent.

These results indicate that the content of flavonoids having antioxidant properties increased through the fermentation process of Leuconostoc mesenteroides GFC 160704 strain (accession number: KCTC18474P), a lactic acid bacteria isolated from ginseng, and thus the efficacy compared to before fermentation. It was judged to have increased.

<Experimental Example 4> Anti-wrinkle efficacy test: Elastase inhibitory activity

The degree of inhibition of Elastase was evaluated using the extracts of Examples 1 to 2 and Comparative Examples 1 to 2.

Examples 1-2 and Comparative Examples 1-2 were diluted to a concentration of 50 (㎍ / ㎖), and then dissolved in 10 mM Tris-HCl buffer (pH 8.0) N-succinyl-(Ala)3-p-nitroanilide (S4760 , Sigma) 1.6 mM substrate and Elastase from porcine pancreas Type IV (E0258, Sigma) 0.4U/mL were mixed and reacted at 25° C. for 5 minutes, and the absorbance was measured at 410 nm by placing in a 96 well plate. At this time, it was carried out using oleanolic acid (Oleanolic acid) as a positive control.

The elastase inhibitory activity was calculated using Equation 3 below using the case where no sample was added as a control.

[Equation 3]

Elastase inhibitory activity (%) = 100- (absorbance of the sample treated group/absorbance of the untreated group X 100)

division Elastase inhibitory activity (%) Positive control 90.300.50 Negative control 6.10.50 Example 1 54.60.43 Example 2 81.30.64 Comparative Example 1 31.20.55 Comparative Example 2 55.00.33

As a result, as shown in Table 3, it was confirmed that Examples 1 to 2 have similar or superior elastase inhibitory activity compared to Comparative Examples 1 and 2. In particular, it was confirmed that the elastase inhibitory activity was significantly increased in Example 2 than in Example 1 among Examples 1 to 2, and the flow fermentation extract, Hwangbaek extract, and Hwangchil extract were used more than when the flow fermentation extract was used alone. When the mixed complex extract was used, it was confirmed that the anti-wrinkle effect was more excellent.

These results confirmed that the wrinkle improvement effect was improved through the fermentation process of Leuconostoc mesenteroides GFC 160704 strain (accession number: KCTC18474P), which is a lactic acid bacteria isolated from ginseng.

<Experimental Example 5> Whitening efficacy test: Measurement of melanin production

Using the extracts of Examples 1 to 2 and Comparative Examples 1 to 2, the amount of melanin production was measured to confirm the effect on the production of melanin related to skin whitening.

B16F10 melanoma cells were counted equally at a rate of 2.0x10 ⁵ cells/well per dish in a 60Φ Petri dish using a hemacytometer, and then incubated for 24 hours at 37°C and 5% carbon dioxide. After culturing for 24 hours, the obtained cell culture solution was removed, Examples and Comparative Examples were taken together with α-MSH 50 nM for inducing melanin production, mixed with a medium, dispensed, and reacted for 60 hours, and then culture solution (supernatant) Collected. The culture solution (supernatant) reacted for 60 hours was taken 1 ml to measure the amount of extracellular melanin produced, and the absorbance was measured at 450 nm using an ELISA plate reader. At this time, arbutin was used as a positive control.

It was calculated using Equation 4 below to confirm the amount of melanin production.

[Equation 4]

Melanin production amount (%) = (absorbance of sample treatment group/absorbance of control group) X100

division Melanin contents (% of control) Positive control 26.520.88 Negative control 92.121.54 Example 1 19.890.37 Example 2 22.741.11 Comparative Example 1 38.810.94 Comparative Example 2 74.511.05

As a result, as shown in Table 4, Examples 1 to 2 were confirmed to have a low melanin production amount compared to Comparative Examples 1 to 2, so that the melanin production inhibitory effect was excellent.

These results confirmed that the whitening effect was enhanced through the fermentation process of Leuconostoc mesenteroides GFC 160704 strain (accession number: KCTC18474P), a lactic acid bacteria isolated from ginseng.

<Experimental Example 6> Anti-inflammatory efficacy test: Nitric Oxide (NO) inhibitory activity

In order to confirm the anti-inflammatory activity effect using the extracts of Examples 1 to 2 and Comparative Examples 1 to 2, the degree of inhibition of nitric oxide was measured.

RAW 264.7 cells, a type of macrophage, were equally counted and dispensed in ²⁴ well plates at 1.0x10 ⁴ cells/well using DMEM medium containing 1% penicillin/scraptomycin and 10% FBS (fetal bovineserum). Incubated for 24 hours at 37°C and 5% carbon dioxide. After mixing Examples 1-2 and Comparative Examples 1-2 with the medium at a concentration of 50 (㎍ / ㎖) to RAW 264.7 cells cultured for 24 hours, 1 ml was added to each well and incubator under conditions of 37°C and 5% CO ₂ Reacted for 24 hours. At this time, LPS (Lipo poly saccharide), which is an inflammation inducing factor expressing NO, was treated at a concentration of 1 μg/ml and reacted for 24 hours at 37° C. and 5% carbon dioxide. Next, after separately taking only the supernatant of each well, 100 ml of the culture solution is taken into a 96 well plate using a Nitric Oxide detection kit in each well, and 50 µl of Griess reagent A (N-1-Naphthylethylenediamine (NEDHC)) and Griess reagent B 50 µl of (Sulfanilamide) was added and reacted for 10 minutes each, and the absorbance was measured at 540 nm using an ELISA plate reader. The concentration of NO in the cell culture was calculated by comparing with the standard curve for each concentration of sodium nitrite (NaNO ₂ ).

division NO concentration (μM) Positive control 16.50.23 Negative control 68.60.39 Example 1 21.90.39 Example 2 17.40.54 Comparative Example 1 69.00.98 Comparative Example 2 57.40.54

As a result, as shown in Table 5, Examples 1 to 2 were found to have a lower concentration of nitric oxide generation compared to Comparative Examples 1 to 2, and thus it was confirmed that the effect of inhibiting the formation of nitric oxide was excellent. .

These results confirmed that the anti-inflammatory effect was improved through the fermentation process of Leuconostoc mesenteroides GFC 160704 strain (accession number: KCTC18474P), a lactic acid bacteria isolated from ginseng.

<Experimental Example 7> Antibacterial efficacy test: MIC test

The degree of antibacterial activity was confirmed using the extracts of Example 1 and Comparative Example 1.

1. Preparation of evaluation medium

The paper disc method was used as the evaluation method, and the standard test strains ( Escherichia coli , ATCC 8739), Staphylococcus aureus , ATCC 6538P, which are standard test strains (Food and Drug Administration and MIC test guide line), were used to evaluate the antibacterial activity. ), was used as received from the pre-sale of Pseudomonas aeruginosa (Pseudomonas aeruginosa, ATCC 9027), Candida albicans (Candida albicans, ATCC 10231) South Korea microbial resources Center (KCTC, Korea) and Korea Culture Center of microorganisms (KCCM, Korea). Of the standard test strains, E. coli, Staphylococcus aureus and Pseudomonas aeruginosa were cultured using TSA medium (Tryptic Soy Agar) medium, and Candida albicans cultured using YM medium (Yeast extract Media) medium.

2. MIC test

After preparing an independent nutrient broth sterilized at 121° C. for 15 minutes, each standard test strain was inoculated at an amount of ³ ×10 ⁵ cfu/ml. Example 1 and Comparative Example 1 were typically set to test concentrations of 1%, 0.5%, 0.25%, 0.125%, 0.0625%, 0.03125%, 0.015625%, and 0.0078125%, and inoculated and cultured using single colony of the test strain. . After the culture was completed, the absorbance (600 nm) was measured to confirm the result. The antimicrobial effect was confirmed at the lowest concentration in which no microorganism was detected.

Classification (Unit: %) Coli Staphylococcus aureus Pseudomonas aeruginosa Candida albicans Example 1 0.25 0.25 0.5 0.25 Comparative Example 1 X 0.1 X X

As a result, as shown in Table 6, Example 1 is a standard test strain (see the Ministry of Food and Drug Safety and MIC test guide line) when the application of 0.25% compared to Comparative Example 1, Escherichia coli , ATCC 8739), Staphylococcus aureus, ATCC 6538P) and Candida albicans (Candida albicans, ATCC have determined the inhibitory effect on 10231), have determined the inhibitory effect at the time of 0.5% applied, Pseudomonas aeruginosa (Pseudomonas aeruginosa, ATCC 9027), the antimicrobial effect is excellent Confirmed.

<Experimental Example 8> Formulation stability evaluation

Using the extracts of Examples 1 to 2 and Comparative Examples 1 to 2, lotions were prepared by a conventional method from the compositions shown in Tables 7 to 10 below.

Ingredient name Content (unit: wt%) Purified water to 100 1,3-butylene glycol 2.0 glycerin 1.0 Carbomer 15.0 Triethanolamine 0.2 Stearic Acid 3.0 Beeswax 0.5 Squalene 5.0 Glyceryl stearate 2.0 Cetyl alcohol 1.0 antiseptic 0.01 Example 1 5.0

Ingredient name Content (unit: wt%) Purified water to 100 1,3-butylene glycol 2.0 glycerin 1.0 Carbomer 15.0 Triethanolamine 0.2 Stearic Acid 3.0 Beeswax 0.5 Squalene 5.0 Glyceryl stearate 2.0 Cetyl alcohol 1.0 antiseptic 0.01 Example 2 5.0

Ingredient name Content (unit: wt%) Purified water to 100 1,3-butylene glycol 2.0 glycerin 1.0 Carbomer 15.0 Triethanolamine 0.2 Stearic Acid 3.0 Beeswax 0.5 Squalene 5.0 Glyceryl stearate 2.0 Cetyl alcohol 1.0 antiseptic 0.01 Comparative Example 1 5.0

Ingredient name Content (unit: wt%) Purified water to 100 1,3-butylene glycol 2.0 glycerin 1.0 Carbomer 15.0 Triethanolamine 0.2 Stearic Acid 3.0 Beeswax 0.5 Squalene 5.0 Glyceryl stearate 2.0 Cetyl alcohol 1.0 antiseptic 0.01 Comparative Example 2 5.0

In order to measure the stability against the degree of separation and discoloration of the lotion containing the extracts of Examples 1 to 2 and Comparative Examples 1 to 2 prepared in Tables 7 to 10, it is kept constant at 45°C, 25°C and 4°C Five lotions containing the extracts of Examples 1 to 2 and Comparative Examples 1 to 2 were each put in a container in a constant temperature bath and sunlight conditions, and stored for 4 weeks, and then the degree of separation and discoloration were compared. At this time, the degree of separation and discoloration of the product was evaluated by classifying it into the following 6 grades.

Separation and discoloration evaluation criteria

0: no change

1: very little separation (discoloration)

2: little separation (discoloration)

3: Slightly severe separation (discoloration)

4: severe separation (discoloration)

5: Extremely severe separation (discoloration)

Evaluation item Degree of separation and discoloration Example Comparative example One 2 One 2 45℃ One One One 2 25℃ 0 0 0 0 4℃ 0 0 0 0 daylight One One One One

As a result, as shown in Table 11, Examples 1 to 2 hardly exhibited discoloration and separation phenomena compared to Comparative Examples 1 and 2, indicating that the formulation stability was excellent.

As described above, specific parts of the present invention have been described in detail, and it will be apparent to those of ordinary skill in the art that these specific techniques are only preferred embodiments, and the scope of the present invention is not limited thereby. will be. Accordingly, it will be said that the substantial scope of the present invention is defined by the appended claims and their equivalents.

Claims (8)

Leuconostoc mesenteroides GFC 160704 strain (accession number: KCTC18474P) was inoculated and fermented in a fluid ( Vernicia fordii ) extract, and obtained by inoculating and fermenting it with an antioxidant, anti-wrinkle improvement including a fermented extract of Vernicia fordii as an active ingredient , Whitening, anti-inflammatory and antibacterial cosmetic composition.

delete The cosmetic composition of claim 1, wherein the cosmetic composition additionally comprises an extract of Hwangbaek ( Phellodendron amurense Rupr ) and an extract of Hwangchil ( Dendropanax mordifera ). The cosmetic composition for antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial.
The antioxidant according to claim 3, wherein the ratio of the fermentation extract, Phellodendron amurense Rupr extract, and the extract of Hwangchil ( Dendropanax mordifera ) is 1:0.01~0.5:0.01~0.5 by weight. , Whitening, anti-inflammatory and antibacterial cosmetic composition.
The cosmetic composition of claim 1, wherein the fluid ( Vernicia fordii ) ferment extract is contained in an amount of 0.03 to 20% by weight based on the total weight of the cosmetic composition.
The method of claim 1, wherein the flow (Vernicia fordii) fermentation extract of pre-fermentation fluid (Vernicia fordii) extract prepared flavonoids (Flavonoid) antioxidant, characterized in that the content is increased 6-7 fold, wrinkle, whitening, anti-inflammatory and antibacterial Cosmetic composition.
The method of claim 1, wherein the cosmetic composition is a skin ointment, cream, soft lotion, nutrient lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner , Astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, eye cream, moisture cream, hand cream, foundation, nutrition essence, sunscreen, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion And antioxidant, wrinkle improvement, whitening, anti-inflammatory and antibacterial cosmetic composition, characterized in that any one formulation selected from the group consisting of a body cleanser.

A cosmetic comprising the cosmetic composition of any one of claims 1, 3, 4, 5, 6 and 7.

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