KR101780863B1 - Cosmetic Composion for Acne Improvement Containing Butanol Fraction of Red Ginseng Ethanol Extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving acne comprising a butanol fraction of red ginseng ethanol extract, wherein a fraction having high antimicrobial activity against acne bacterium can be obtained by fractionating the red ginseng ethanol extract with butanol. Therefore, The butanol fraction of the extract can be used in a cosmetic composition requiring an acne scarring effect.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a cosmetic composition for improving acne, which comprises a butanol fraction of a red ginseng ethanol extract,

The present invention relates to a cosmetic composition for improving acne comprising a butanol fraction of red ginseng ethanol extract.

Acne is an inflammatory disease of the hair follicle that shows various skin lesions including facial, thoracic, back and arm open or closed skin, papules, cysts and nodules. Often acne causes a small size rash. These rashes are considered to be a characteristic symptom of acne.

The cause of acne has not yet been clarified, but it is presumed that various causes are involved. However, there are two factors that are caused by the hyperactivity of gonadal function, which is basically caused by hormones and genetic background, and by the action of acne bacteria that break down sebaceous glands and produce free fatty acids by breeding in fur branch lines. Particularly, sebaceous stasis in the hair follicles blocks the air circulation by blocking the hair follicles, and thus the environment in which the anaerobic bacteria residing in the hair follicles can grow well inside the hair follicle. The cotton fabric thus produced is used to create a living environment of microorganisms, such as P. acnes , Staphylococcus epidermidis , and Pityrosporum ovale . These are lipophilic microorganisms. Staphylococcus epidermidis is aerobic and grows in the middle of the outer capsule or hair follicle. Propionibacterium acnes is anaerobic and grows inside the hair follicle.

Until now, research on acne treatment has focused on the exfoliation of pores using salicylic acid and vitamin A derivatives, retinoic acid, and the enhancement of sterility against acne bacteria using antibiotics such as triclosan.

Triclosan has been reported to cause skin irritation when contacted with skin, and many recent studies are raising the interest that triclosan is likely to increase the resistance of bacteria to antibiotics. Since triclosan kills bacteria in a manner similar to antibiotics, there is a growing concern about the abuse of triclosan because bacteria already resistant to triclosan are more likely to be resistant to other antibiotics. In fact, a number of mutation strains resistant to triclosan and some antibiotics have been found in experimental studies.

On the other hand, the physiological activity of ginseng is due to saponin, polysaccharides and peptides, and many studies have been conducted centering on ginsenoside. Thus, the central nervous system control, memory, improvement of learning efficacy and antistress action, As the various pharmacological actions such as proliferation and transfer inhibition action, antioxidative action, antidiabetic action, liver function improvement, radiation protection action and inhibition of thrombogenesis have been scientifically proved, the demand for medicines and functional supplements Is increasing. The efficacy associated with ginseng skin is to improve wrinkles of the skin or to protect against skin damage from exposure to ultraviolet light. Although it has been found that a mixture containing ginseng natural fermentation polysaccharide is effective for the treatment of acne (Patent Document 1), there has not been confirmed the effect of the extract of red ginseng, a ginseng processed product, on the killing action of acne.

The present inventors have studied a raw material which shows excellent antimicrobial effect to acne bacteria similar to triclosan but does not induce skin irritation and does not cause resistance to germs. The fraction obtained by fractionating red ginseng extract as a specific solvent is an acne bacteria The present invention has been completed.

Korean Patent Publication No. 2014-0141080

It is an object of the present invention to provide a composition for improving acne comprising a butanol fraction of red ginseng ethanol extract.

In order to achieve the above object, one aspect of the present invention provides a cosmetic composition for improving acne comprising a butanol fraction of red ginseng ethanol extract.

The term "extract " used in the present invention means a substance extracted from a raw material by an arbitrary method. The extracted extract, the concentrate obtained therefrom, the dried product and the powder of the concentrate are used without limitation.

The term "acne " as used herein refers to any one selected from the group consisting of Propionibacterium acnes, Staphylococcus epidermidis and Pityrosporum ovalet; Or all other skin conditions such as purulent pimples, nodular acne and cystic acne caused by or partially induced by other microorganisms capable of producing acne, as well as closed and open skin.

The term " treatment "as used herein refers to the treatment or prevention of acne, which is a result of application of the pharmaceutical composition of the present invention to an acne site, as well as a cure for acne symptoms as well as a partial cure for acne symptoms, Prevention and " prevention "refers to the application of the pharmaceutical composition of the present invention to a body part to inhibit or prevent the infection or growth of acne bacteria such as Propionibacterium acnes and prevent acne symptoms from occurring in advance .

The term "cosmetic composition" referred to herein includes detergents and cosmetics as a composition used in the human body to cleanse and polish the body to brighten the appearance or to maintain or promote the health of skin and hair .

Hereinafter, the present invention will be described in detail.

One aspect of the present invention provides a cosmetic composition for improving acne comprising a butanol fraction of red ginseng ethanol extract.

The extract may be obtained by extracting from a raw material or a dried product thereof, and the raw material of the extract may be cultivated or commercially available.

When the extract is obtained by extracting from the raw material, any conventional extraction methods known in the art such as a solvent extraction method, an ultrasonic extraction method, a filtration method and a reflux extraction method may be used as the extraction method. Preferably, the extraction method or the reflux extraction method can do. The extraction process can be repeated several times, and then the step such as concentration or freeze-drying can be further roughened. Specifically, the obtained extract is concentrated under reduced pressure to obtain a concentrated liquid, the concentrated liquid is lyophilized, and a concentrated powder can be produced using a pulverizer.

The extraction temperature may be room temperature (about 15-25 ° C) to 80 ° C, preferably room temperature to 77 ° C, more preferably room temperature to 75 ° C, but is not limited thereto. When extracted at a temperature lower than the lower limit value, the antimicrobial effect of acne may be low. If the product is extracted at a temperature exceeding the upper limit value, the component that exhibits the antimicrobial effect may be denatured by heat and the antimicrobial effect of acne may be lowered.

The extraction time may be 10 minutes to 36 hours, but is not limited thereto. When extracted at a time lower than the lower limit value, the active ingredient may not be extracted sufficiently, and when extracted at an amount exceeding the upper limit value, the active ingredient exhibiting an antibacterial effect against acne bacteria may be denatured.

The extract can be extracted using ethanol as an extraction solvent. The ethanol may be an aqueous solution of ethanol. The ethanol may be 50 to 100% (v / v), preferably 50 to 90% (v / v) and more preferably 50 to 85% (v / v) Not limited. When the concentration of the aqueous ethanol solution is less than 50% (v / v), the effect of the acne scavenging effect is relatively lower as compared with the case of extracting the ethanol with a proper amount.

In a specific example of the present invention, 55% (v / v) ethanol is used as an extraction solvent when the remaining portion of red ginseng is used as a raw material, and each extract obtained by continuous extraction at 40 ° C or 75 ° C one time or more, It is effective.

In another embodiment of the present invention, when the entire red ginseng roots are used as a raw material, 70% (v / v) or more of ethanol is used as an extraction solvent, and the extracts obtained by extracting once at 40 캜 or 75 캜, It is effective. When the whole red ginseng root is used as a raw material and 55% ethanol is used as an extraction solvent, the antibacterial effect is low.

When the butanol fraction of red ginseng ethanol extract is prepared, the red ginseng ethanol extract may be diluted when the concentrate is used. The diluted ginseng ethanol extract is mixed with butanol. The mixing may be conducted at room temperature (about 15 to 25 DEG C), but is not limited thereto. The prepared mixture can be separated using a fraction funnel, and the temperature at which the separation is carried out may be carried out at room temperature (about 15 to 25 ° C), but is not limited thereto. The separation time can be from 10 minutes to 48 hours, but is not limited thereto. After the separation is completed, the upper layer of the separated layer is recovered to obtain a butanol fraction of the red ginseng ethanol extract. The obtained butanol fraction is completely evaporated, then purified water is added thereto, and after freezing at low temperature, it is put into a freeze dryer, and dried and stored.

The butanol may be contained in an amount of 0.01 to 10 parts by weight based on 1 part by weight of the red ginseng ethanol extract, preferably 0.05 to 5 parts by weight, and more preferably 0.1 to 3 parts by weight of butanol. But is not limited thereto. When butanol is added at a ratio lower than the lower limit described above, the antibacterial effect of the acne bacteria in the final fraction may be low. In addition, when butanol is added in an amount exceeding the upper limit value, a considerable amount of time and expense is required to evaporate the butanol solvent from the butanol fraction, thereby causing environmental pollution.

The butanol fraction of the red ginseng ethanol extract may be contained in the cosmetic composition for improving acne in an amount of 0.01 to 30% by weight, preferably 0.01 to 20% by weight, more preferably 0.01 to 10% by weight , And even more preferably from 0.01 to 5% by weight. If the content is less than 0.01% by weight, the effect of killing an acne bacterium may be insignificant. If the content is more than 30% by weight, the effect of killing the acne bacterium is relatively low and the viscosity of the formulation is excessively lowered, It can cause trouble to the skin.

In the cosmetic composition of the present invention, the fraction may be prepared into a liquid or a solid form using bases, adjuvants and additives commonly used in the cosmetics field. Liquid or solid cosmetics may include, for example, but not limited to, lotions, creams, lotions, bath salts, and the like.

The base, adjuvant and additives commonly used in the cosmetics field are not particularly limited, and examples thereof include water, alcohol, propylene glycol, stearic acid, glycerol, cetyl alcohol, liquid paraffin and the like.

When the composition of the present invention is prepared with a cosmetic composition, the composition of the present invention may contain not only the butanol fraction of the red ginseng ethanol extract, but also components commonly used in cosmetic compositions such as antioxidants, stabilizers, Vitamins, pigments and flavoring agents, and carriers.

The cosmetic composition of the present invention may be prepared in any form conventionally produced in the art and may be in the form of a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant- Oils, powder foundations, emulsion foundations, wax foundations and sprays, but are not limited thereto. More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition having an acne-killer effect according to the present invention can be used according to a conventional method of use, and may be used in a number of times depending on the skin condition or taste of the user.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

The butanol fraction of the red ginseng alcohol extract according to the present invention is excellent in the antimicrobial effect of acne bacteria and thus can be used in a cosmetic composition for improving acne.

Hereinafter, the present invention will be described in detail with reference to examples.

However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention by the following production examples, examples and experimental examples.

Of red ginseng ethanol extract Fraction  Produce

An aqueous solution of 70% (v / v) or more ethanol was added to 1 kg of red ginseng in 7 times, and extracted three times at 40 ° C for 8 hours. The extract was extracted with 50 g or more of a red ginseng ethanol extract concentrate having a moisture content of 40% by using a reduced pressure concentrator. 50 g of the obtained concentrate was diluted with 100 g of purified water and stored at room temperature for 24 hours. The diluted concentrate was mixed with butanol, ethyl acetate, and hexane at a ratio of 3: 2, respectively. The mixture of the diluted concentrate and the solvent was put in a fraction funnel and separated at room temperature for 24 hours. The supernatant of the separated layer was recovered and the solvent was completely evaporated. Then, purified water was added thereto, and the resultant was frozen at -70 ° C., then placed in a freeze dryer for 72 hours and dried to obtain solid fractions of butanol fraction, ethyl acetate fraction and hexane fraction.

Fraction  Comparison of Antimicrobial Effect against Acne Bacteria by Different Types

The butanol fraction, ethylacetate fraction and hexane fraction obtained in Example 1 above were confirmed to have an acne fungicidal effect. The solid contents of the fractions obtained in Example 1 were dissolved in purified water at 100, 50 and 25 mg / ml, respectively, and the antimicrobial activity against acne bacteria was confirmed by the paper disk method. Propionibacterium acnes (ATCC 6919), a skin-borne bacterium that causes acne, was cultured in a Reinforced clostridial medium (difco, USA). Reinforced clostridial medium P. acne prepared on the plate medium was inoculated evenly with a sterile cotton swab. Clear zone, a range in which acne bacteria do not grow, was measured and the effect of each sample was compared. As a positive control, tea tree oil (100 mg / ml) and triclosan (3 mg / ml) used as acne antimicrobial agents were used.

Clear zone (mm)   Sample concentration 100 mg / ml 50 mg / ml 25 mg / ml Red ginseng ethanol extract concentrate 6 6 4 Hexane fraction 3 2 2 Ethyl acetate fraction 2 1.5 One Butanol fraction 8 7 6.5 Tea trioyl (100 mg / ml) 2.0 Triclosan (3 mg / ml) 14.0

As a result, the butanol fraction showed higher antimicrobial activity against acne than 33% (100mg / ㎖), 16.6% (50mg / ㎖) and 62.5% (25mg / ㎖) The clear zone was found to be about 4 times wider than that of the tea tree oil. In addition, the butanol fraction exhibits antimicrobial activity against acne of 46 ~ 57% as compared with triclosan, so that it is expected to effectively replace triclosan.

Butanol of red ginseng ethanol extract Fraction  Confirmation of antibacterial effect

To determine whether the butanol fraction of the present invention has an antimicrobial effect against other microorganisms, S. mutans (using Brain heart infusion agar), E. coli (ATCC) (Trypticase Soy Agar use), Staphylococcus aureus (S.aureus) (Korea Research Institute of Bioscience and biotechnology) (using Trypticase Soy Agar), yeast (C. albicans) (Korea Research Institute of Bioscience and biotechnology) (potato dextrose agar used) and fungus (A.niger) (Korea Research Institute of Bioscience and Biotechnology) (using potato dextrose agar) and the culture medium corresponding to each microorganism was used, the antibacterial effect of each microorganism was confirmed in the same manner as in Example 2.

Clear zone (mm) Sample type Butanol fraction of red ginseng ethanol extract Tea trio day Triclosan Treatment concentration 50 mg / ml 25 mg / ml 12.5 g / ml 6.25 g / ml 100 mg / ml 3 mg / ml Cavities 0 0 0 0 0 6 Escherichia coli 0 0 0 0 0 21 Staphylococcus 0.5 0 0 0 0 23 yeast fungus 0 0 0 0 0 7 Fungus 0 0 0 0 0 5

As a result, the butanol fraction of the red ginseng ethanol extract showed no antimicrobial effect at a concentration of less than 25 mg / ml and no antimicrobial effect even at a concentration of 50 mg / ml against chitin, E. coli, staphylococcus, yeast, and fungus. From the above results, it was found that the butanol fraction of the present invention has an antibacterial effect specifically for acne bacteria than other bacteria or fungi.

Identify ingredients that show antimicrobial activity

Saponin and antimicrobial components of red ginseng were separated in order to confirm whether the antimicrobial effect confirmed in Example 2 was caused by saponin or by an ingredient other than saponin. In order to separate saponin and antimicrobial components from red ginseng, the following experiment was designed.

100 g of water was mixed with 50 g of the red ginseng ethanol concentrate obtained in Example 1 and poured into an HP-20 column. Then, 1 L of purified water was sequentially poured into the column, and then 30% (v / v) aqueous ethanol solution was poured into the column to obtain a fraction. The fractions were separated into 50% (v / v) and 95% (v / v) ≪ / RTI > (Fraction obtained from purified water), 3070.9 / / ml (fraction obtained from a 30% (v / v) aqueous ethanol solution of purified water fraction), 6463.4 / / (Fraction obtained from 50% (v / v) ethanol aqueous solution of 30% (v / v) ethanol fraction), 2630.6 / / ml (fraction obtained from 50% (v / v) ethanol fraction) Fractions obtained) were confirmed.

The above results show that saponin almost excreted in 50% (v / v) ethanol fraction and 95% (v / v) ethanol fraction showed some hydrophobic substance with 50% (v / v) ethanol fraction together with some saponin do. Based on these four samples, the antibacterial effect against acne was confirmed in the same manner as in Example 3, and the following results were obtained.

Clear zone (mm) Saponin content
(Unit: 占 퐂 / ml) Treatment concentration 50 mg / ml 25 mg / ml 12.5 mg / ml Fractions obtained from purified water 3 2.5 1.5 574 The 30% (v / v) ethanol fraction of the 0% ethanol fraction 3 2 1.5 3070.9 (V / v) ethanol fraction of the 0, 30% (v / v) ethanol fraction 3 2 One 6463.4 (V / v) ethanol fraction of 0, 30% (v / v), 50% (v / v) 6 5 4.5 2630.6

(V / v) ethanol fraction, which is significantly lower than the ethanol fraction from 0 to 50% (v / v), is similar to the antioxidant activity in the 0 to 50% (v / v) ethanol fraction irrespective of the saponin content of red ginseng. The ethanol fraction showed much higher antimicrobial activity than the other fractions. These results show that saponin does not show antimicrobial effect of acne but is caused by hydrophobic substances other than saponin.

Cosmetics  Preparation of composition

5-1. Manufacture of essences

The butanol fraction of the red ginseng ethanol extract of Example 1 was used to prepare essences according to the content (parts by weight) shown in Table 4 below.

Furtherance Content (parts by weight) Triethanolamine 0.25 Carboxyvinyl polymer 0.22 glycerin 4 Butylene glycol 2 The butanol fraction of Example 1 1.5 Wax 0.5 Cetostearyl alcohol One Glyceryl monostearate One Squalene 4 Purified water Suitable amount

5-2. Manufacture of softening longevity

The softening water containing the butanol fraction of the red ginseng ethanol extract of Example 1 as an active ingredient was prepared as shown in Table 5 below.

Raw material Content (parts by weight) 1,3-butylene glycol 1.00 Disodium iodide 0.05 Allantoin 0.10 Dipotassium glycyrrhizate 0.05 Citric acid 0.01 Sodium citrate 0.02 Glycereth-26 1.00 Arbutin 2.00 PEG-40
Hydrogenated castor oil 1.00 ethanol 30.00 The butanol fraction of Example 1 0.5 coloring agent a very small amount Flavoring agent a very small amount Purified water Balance

5-3. Manufacture of nutrition cream

The nutritional cream containing the butanol fraction of the red ginseng ethanol extract of Example 1 as an active ingredient was prepared as shown in Table 6 below.

Raw material Content (parts by weight) 1,3-butylene glycol 7.0 glycerin 1.0 D-Panthenol 0.1 Magnesium aluminum silicate 0.3 PEG-40 stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Chin type glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl alcohol 3.0 Mineral oil 4.0 Squalene 3.8 Example 1 Butanol fraction 1.5 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycyrrhizate a very small amount Allantoin a very small amount Sodium hyaruronate a very small amount Tocopheryl acetate Suitable amount Triethanolamine Suitable amount Flavoring agent Suitable amount Purified water Balance

5-4. Manufacture of lotions

Lotions containing the butanol fraction of the red ginseng ethanol extract of Example 1 as an active ingredient were prepared as shown in Table 7 below.

Raw material content( Weight portion ) Cetostearyl alcohol 1.6 Stearic acid 1.4 Pro-type glycerin monostearate 1.8 PEG-100 stearate 2.6 Sorbic acid sesquioleate 0.6 Squalene 4.8 Macadia oil 2 Jojoba oil 2 Tocopheryl acetate 0.4 Methylpolysiloxane 0.2 Tocopheryl acetate 0.4 1,3-butylene glycol 4 Xanthan gum 0.1 glycerin 4 d-Pandenol 0.15 The butanol fraction of Experimental Example 1 1.0 Allantoin 0.1 Carbomer (2% aq. Sol) 4 Triethanolamine 0.15 ethanol 3 Purified water Suitable amount

Claims (7)

Preparing an extract of red ginseng ethanol; And
A cosmetic composition for improving acne comprising a butanol fraction of red ginseng ethanol extract prepared by a process comprising only fractionating the red ginseng ethanol extract with butanol,
The ethanol is 50 to 100% (v / v)
Wherein the butanol is contained in an amount of 0.1 to 3 parts by weight based on 1 part by weight of the red ginseng ethanol extract. delete delete delete delete The method according to claim 1,
Wherein the butanol fraction of the red ginseng ethanol extract is 0.01 to 30 wt% in the cosmetic composition for improving acne. The method according to claim 1,
The acne-improving cosmetic composition may be any one selected from the group consisting of formulations of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, A cosmetic composition for improvement.