KR102023473B1 - Method of Preparing Red Ginseng Fractions having Enhanced Cosmetic Components - Google Patents

Abstract

The present invention relates to a method of preparing a red ginseng fraction with enhanced cosmetic ingredients, the fraction prepared by the method of the present invention is superior to the simple red ginseng extract or fractions fractionated under other conditions, and has an excellent antibacterial effect against acne bacteria. In addition, since the whitening effect is excellent and various functions can be simultaneously exhibited, the fraction of the present invention can be used in cosmetic compositions for acne improvement or whitening purposes.

Description

Method of Preparing Red Ginseng Fractions having Enhanced Cosmetic Components

The present invention relates to a method of preparing a fraction of the red ginseng extract with enhanced cosmetic ingredients and a fraction of the red ginseng extract prepared by the method, the fraction of the red ginseng extract of the present invention has an antibacterial effect against acne bacteria, whitening effect There is.

Acne is an inflammatory disease of the hair follicle sebaceous glands that show various skin lesions such as closed or open scalp, papules, cysts and nodules in the face, chest, back and arms, but the cause of acne has not been clearly identified. Presumed to be involved. However, there are two main factors that are caused by the action of acne, which is caused by hyperactivity of the gonads, which are thought to be caused by hormones and genetic backgrounds, and by breeding in the fur glands, breaking down sebum and producing free fatty acids. In particular, sebum stagnated in the hair follicles block the hair follicles to block the circulation of air, so inside the hair follicles anaerobic bacteria that reside in the hair follicles can grow well. The resulting cotton fabric is to include propynyl sludge tumefaciens arc Ness (P. Acnes), Staphylococcus epidermidis (Staphylococcus epidermidis), and error correcting capability of Ross Forum Ovalle (Pityrosporum ovale) growth to create the living environment of the microorganisms. These are fat-compatible microorganisms, Staphylococcus epidermidis, aerobic, growing in the middle of hair follicles or hair follicles, and propionibacterium acnes are anaerobic and grow inside hair follicles.

Until now, research on acne has been focused on the removal of dead skin pores using salicylic acid and retinoic acid preparations, which are vitamin A derivatives, and the enhancement of bactericidal activity against acne bacteria using antibiotics such as triclosan. However, triclosan has been reported to cause skin irritation upon skin contact, and another study found that triclosan is likely to increase bacterial resistance to antibiotics. Triclosan kills bacteria in a similar way to antibiotics, so bacteria that have already been resistant to triclosan are more likely to be resistant to other antibiotics, so there is much concern about abuse of triclosan.

On the other hand, one of the cosmetics fields of greatest interest to women is the field of skin whitening. "Skin whitening" is to adjust the skin color caused by excessive production of pigments in the skin due to environmental changes such as ultraviolet rays, stress, hormone secretion abnormalities and pregnancy. The most important factor in this regard is the skin pigment melanin. Melanin is synthesized in melanocytes, which are pigment cells in the basal layer of the epidermis, and transferred to the surrounding keratinocytes to determine skin color. Melanin is an unusually stable pigment found in living organisms, insoluble in almost all solvents, and is difficult to extract or chemically handle. Because of this, there are many unknowns about the chemical aspects of melanin and the progress of research is sluggish.

The melanin synthesis process is clearly shown to occur in the organelles of the melanosomes of specially differentiated cells called melanocytes. That is, the skin color is determined according to the content, distribution, and the like of melanin and is related to the number and distribution of melanosomes generated in melanocytes and released to the outside of cells. Hyperpigmentation of the skin can be caused by a number of factors, including hormonal abnormalities, genetic diseases, and UV irradiation after the inflammatory response of the skin. The main factors are due to melanin synthesis abnormalities and distribution abnormalities. The main function of melanin is to remove oxygen radicals to protect the skin from damage. The high levels of melanin mean an effective response system to protect the skin from physical and chemical toxic substances.

Excessive melanin deposition is a cause of pathological problems such as skin, but is recognized as a cosmetic problem such as spots, freckles, spots and blotch, thus increasing interest in whitening agents that can inhibit melanin formation. There is much research going on.

Meanwhile, the physiological activity of ginseng is caused by saponins, polysaccharides, and peptides, and many studies have been conducted around ginsenosides, and various pharmacological actions have been scientifically proven. The demand is increasing. Although a mixture containing ginseng natural fermentation polysaccharide has been found to be effective in the treatment of acne (Patent Document 1), the ginseng-derived red ginseng extract has not confirmed the killing effect of acne bacteria. In addition, Patent Literature 2 discloses a technique in which oil extracted from ginseng seeds has a skin whitening effect, but does not disclose that the red ginseng extract is further processed to obtain a fraction having excellent whitening effect.

The present inventors studied a raw material that can exhibit various effects at the same time, while showing excellent antibacterial effect against acne bacteria, does not cause skin irritation, does not cause resistance to bacteria, and can also show a whitening effect, red ginseng The present invention was completed by confirming that the fractions of the extract were subjected to HPLC under specific conditions to have an excellent antibacterial effect against acne bacteria and also an excellent whitening effect.

Korean Patent Publication No. 2014-0141080 Korean Patent Registration No. 1477073

In order to solve the skin irritation and bacterial resistance that may occur when the synthetic compound is used as an antimicrobial against the acne bacteria, natural ingredients exhibiting an antimicrobial effect were searched. In addition, it is to provide a natural raw material having a variety of functions by checking whether the natural raw material exhibiting an antimicrobial effect has other skin cosmetic effects in addition to the antibacterial effect.

In order to solve the above problems, the present invention provides a method for preparing an HPLC fraction of red ginseng extract comprising the following steps:

Purifying the butanol fraction obtained by fractionating the red ginseng water extract with butanol and separating only the butanol layer, or the column purified fraction obtained by column purification fractionating the red ginseng water extract by HPLC.

The present invention also provides an HPLC fraction of the red ginseng extract prepared by the above production method.

The present invention also provides an antimicrobial cosmetic composition comprising an HPLC fraction of the red ginseng extract.

In addition, the present invention provides a cosmetic composition for whitening comprising an HPLC fraction of the red ginseng extract.

Fractions of the red ginseng extract prepared by the production method of the present invention is excellent in antibacterial effect against acne bacteria, and also excellent in whitening effect can simultaneously exhibit various functions, the fraction of the present invention for improving acne or whitening cosmetics It can be used for the composition.

1 shows a method for preparing a fraction (experimental group) of the red ginseng extract of the present invention: the other fraction (control group 2) shows the water layer after obtaining a butanol layer after the butanol fraction.
Figure 2 shows the whitening effect of the fraction (experimental group) of the red ginseng extract of the present invention.

Hereinafter, terms used in the present invention will be described.

As used herein, the term "extract" refers to a material extracted by any method from raw materials, and is used to include all extracts, concentrates obtained therefrom, dried products of the concentrates, and powders without limitation.

The term “acne” as used herein is typically any one selected from the group consisting of propionibacterium acnes, staphylococcus epidermidis and phytilosporum obale; Or all skin conditions, such as purulent acne, nodular acne and cystic acne, caused by or partially caused by other microorganisms that can cause acne.

In the present invention, "acne improvement" is a term that includes the treatment or prevention of acne, the "treatment" is a result of applying the pharmaceutical composition of the present invention to the acne site, the cure of acne symptoms as well as the partial cure of acne symptoms, Improvement and alleviation, and the "prevention" is to prevent or prevent acne symptoms by applying the pharmaceutical composition of the present invention to the body part to inhibit or block acne bacteria infection or growth, such as propionibacterium acnes I mean.

The "cosmetic composition" as referred to herein is a composition used in the human body to clean and beautify the human body to brighten the appearance, or to maintain or promote the health of the skin and hair, and includes detergents and cosmetics. .

Hereinafter, the present invention will be described in detail.

The present invention provides a method for preparing an HPLC fraction of red ginseng extract comprising the following steps:

Purifying the butanol fraction obtained by fractionating the red ginseng water extract with butanol and separating only the butanol layer, or the column purified fraction obtained by column purification fractionating the red ginseng water extract by HPLC.

The extract may be obtained by extracting from a raw material or a dried product thereof, and the raw material of the extract may be used without limitation, such as being grown or commercially available.

When the extract is obtained by extracting it from raw materials, all conventionally known extraction methods such as solvent extraction, ultrasonic extraction, filtration and reflux extraction can be used as extraction methods, and preferably prepared by using solvent extraction or reflux extraction. can do. The extraction process may be repeated several times, after which it may be further subjected to steps such as concentration or lyophilization. Specifically, the obtained extract may be concentrated under reduced pressure to obtain a concentrate, and the extract may be lyophilized to prepare a high concentration of extract powder using a grinder.

When the red ginseng water extract is fractionated into butanol, the red ginseng water extract may be used in an amount of 10 parts by weight, and the butanol may be used in an amount of 0.1 to 25 parts by weight, and preferably, the butanol is in an amount of 1 to 20 parts by weight based on 10 parts by weight of the red ginseng water extract. The butanol may be used in an amount of 5 to 15 parts by weight based on 10 parts by weight of the red ginseng water extract, but is not limited thereto. The butanol may be an aqueous butanol solution, preferably saturated butanol, but is not limited thereto. When the butanol is included below the lower limit, the active ingredient may not be sufficiently fractionated. When the butanol is included above the upper limit, a large amount of time and cost are required to evaporate the butanol solvent from the butanol fraction. There is a problem.

The red ginseng water extract may be used as a filler in the column purification fraction, and a filler such as silica gel, activated alumina, synthetic polymer, magnesium silicate, activated carbon, cellulose, ion exchange resin, and silica gel or aromatic synthetic resin may be used as a filler. When the aromatic synthetic resin is used, it may be an aromatic type synthetic adsorbent column, and more preferably, Diaion HP-20 synthetic adsorbent is used as a filler, but is not limited thereto. Separation using the column may be carried out once to several times until the fraction of desired purity is purified, and may be concentrated and recrystallized as necessary.

The desorption solvent in the preparation of the purified fraction may be any one selected from the group consisting of water, organic solvents and mixtures thereof. The organic solvent is alcohol, hexane (n-hexane), ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane, chloroform, ethyl acetate, benzene, acetone, acetonitrile and mixed solvents thereof. It may be any one selected from the group consisting of, preferably the organic solvent may be any one selected from the group consisting of acetone, acetonitrile, C ₁ to C ₄ alcohol, and a mixed solvent thereof, more preferably The organic solvent may be ethanol, but is not limited thereto.

When the desorbing solvent is ethanol when preparing the purified fraction, the desorption solvent may be ethanol or an aqueous ethanol solution, and the desorption solvent may be an aqueous ethanol solution of less than 40 to 100% (v / v), preferably 45 to It may be an aqueous solution of ethanol of less than 100% (v / v), more preferably less than 50 to 100% (v / v) of an aqueous solution of ethanol, but is not limited thereto. When the concentration of the ethanol aqueous solution used as the desorption solvent is less than the lower limit, there is a problem that the antibacterial or whitening effect of the fraction obtained is lowered.

When the ethanol aqueous solution, which is a desorption solvent, is added during the preparation of the purified fraction, it includes flowing an aqueous ethanol solution to the column, for example, by pressing it under pressure.

When the butanol fraction or the column purified fraction is purified by HPLC, it may be sequentially developed while giving a concentration gradient to a developing solvent (water: acetonitrile = 5 to 35:65 to 95) in which water and acetonitrile are mixed. The HPLC fractionation step may be to recover the peak portion appearing when using a developing solvent (water: acetonitrile = 5 ~ 25:75 ~ 95) mixed with water and acetonitrile, preferably water and acetonitrile mixed It may be to recover the peak portion that appears when using a developing solvent (water: acetonitrile = 10 to 20: 80 to 90), more preferably, a developing solvent (water: acetonitrile = 10: 90 mixed with water and acetonitrile) May be to recover the peak portion appearing.

In one embodiment of the present invention, the butanol layer obtained after the butanol fraction of the red ginseng water extract, or the column fraction of the red ginseng water extract were subjected to HPLC, respectively, and a developing solvent in which water and acetonitrile were mixed (water: acetonitrile = 10: 90) confirmed that each fraction was excellent in the antibacterial effect against acne bacteria, and the effect of inhibiting the production of melanin pigment.

The present invention also provides an HPLC fraction of the red ginseng extract prepared by the above production method.

The present invention also provides an antimicrobial cosmetic composition comprising an HPLC fraction of the red ginseng extract. The antimicrobial cosmetic composition of the present invention is excellent in antimicrobial activity and very excellent in acne improvement. In addition, by inhibiting the activity of acne bacteria, the skin soothing effect is very excellent. In particular, the antimicrobial cosmetic composition of the present invention can be significantly improved skin improvement effect compared to the cosmetic composition containing a simple red ginseng extract or fractions fractionated under other conditions.

In addition, the present invention provides a cosmetic composition for whitening comprising an HPLC fraction of the red ginseng extract. The cosmetic composition for whitening of the present invention provides excellent whitening effect by fundamentally inhibiting melanin production, can improve skin pigmentation and skin tone down phenomenon, and is effective in reducing skin angular mass. Therefore, the cosmetic composition for whitening of the present invention can be effectively used to comprehensively manage skin spots and skin tones such as blemishes and blemishes.

In the cosmetic composition of the present invention, the fraction may be prepared in liquid or solid form using bases, adjuvants and additives commonly used in the cosmetic field. Cosmetics in liquid or solid form may include, but are not limited to, for example, cosmetics, creams, lotions, baths and the like.

Bases, adjuvants and additives commonly used in the cosmetic field are not particularly limited and include, for example, water, alcohols, propylene glycol, stearic acid, glycerol, cetyl alcohol, liquid paraffin and the like.

When the composition of the present invention is made into a cosmetic composition, the composition of the present invention may include not only the purified fraction of red ginseng water extract, but also components commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, Conventional adjuvants such as vitamins, pigments and flavorings, and carriers.

Cosmetic compositions of the invention can be prepared in any formulations conventionally prepared in the art, including, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethylcarbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention may be used alone or in duplicate, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition excellent in killing acne bacteria according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel and cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are only for illustrating the present invention, and the content of the present invention is not limited by the following preparation examples, examples and experimental examples.

Preparation Example 1 Preparation of Fractions of Red Ginseng Extract

The red ginseng extract was identified whether the fractions obtained under specific conditions had skin cosmetic effects such as antibacterial and whitening effects as compared to the simple red ginseng extract or fractions obtained under other conditions.

<1-1> Butanol Fraction of Red Ginseng Water Extract

Water was added to the red ginseng powder 10 times and extracted three times for 8 hours at around 70 ° C. The extract was obtained by using a vacuum condenser to obtain more than 2kg of red ginseng water extract concentrate containing less than 30% moisture content. 2 kg of the obtained concentrate was diluted in 5 L of distilled water and then stored at room temperature for 24 hours. The diluted concentrate was mixed with saturated butanol in equal proportions. The mixture of diluted concentrate and solvent was placed in a fraction funnel and separated for 24 hours at room temperature. After recovering the upper part of the separated layer, the solvent was completely evaporated, purified water was added thereto, frozen at −70 ° C., and then placed in a freeze dryer for 72 hours to obtain 215 g of a solid (control 1) of the butanol fraction. The lower layer (water layer) of the separated layer was obtained separately (control 2).

<1-2> Preparation of Column Purified Fractions of Red Ginseng Water Extract

2 kg of the red ginseng water extract concentrate obtained as in Preparation Example 1-1 was diluted in 5 L of distilled water and loaded into a column packed with HP-20 25 L, followed by 20 L of purified water and 20 L of 30% (v / v) ethanol aqueous solution. Was added to obtain the dissolved component separately. Thereafter, 20L of 50% (v / v) ethanol and 20L of 100% (v / v) ethanol were added to obtain the dissolved component. After evaporating the solvent completely, purified water was added and the mixture was frozen at -70 ℃ for 72 hours. It was put into a freeze dryer and dried to obtain 150g of solid content.

<1-3> HPLC Fraction of Red Ginseng Extract

HPLC was performed on the butanol fractions or column purified fractions obtained in Production Examples <1-1> and <1-2>, respectively. HPLC conditions are as follows.

Device used: YMC LC-Forte / R

Column Used: YMC Actus Triart-C18 (250 X 30mm)

Run time: 90 min

Flow rate: 30ml / min

Injection volume: 10ml

Solvent Conditions: H ₂ 0: Acetnitrile = 80:20-> 10:90

Only fractions obtained under the condition that H ₂ 0: Acetonitrile = 10: 90 during HPLC under these conditions were obtained (experimental group). The HPLC fraction obtained from the butanol fraction obtained in Production Example <1-1> was named Experimental Group 1, and the HPLC fraction obtained from the column purified fraction obtained in Production Example <1-2> was named Experimental Group 2. In addition, as a control 3 fractions were obtained under the conditions H ₂ 0: Acetonitrile = 68: 32 ~ 72: 28. The extraction / fractionation method of Experimental Group 1 and Experimental Group 2, Control Group 1 to Control Group 3 obtained in the present invention is shown in FIG. 1.

Example 1 Antibacterial Effect Experiment

The antimicrobial effect of P. acnes, known to cause acne on the skin, was confirmed by the paper disc method. Propionibacterium acnes (ATCC 6919) was used as an acne-causing cause of acne and was cultured in Reinforced clostridial medium (difco, USA). P. acne bacteria solution prepared on Reinforced clostridial medium plate medium was evenly inoculated with a sterile swab. Clear zone, which is a range in which acne bacteria cannot grow, was measured to compare the effect of each sample. As a sample, Experimental Group 1 and Experimental Group 2, which are HPLC fractions obtained in Preparation Example <1-3>, were used, and Controls 1-3 were used as comparative examples of the experimental group. The results are shown in Table 1.

(mg / ml) 50 25 12.5 Red ginseng water extract 0 0 0 Control group 1 0 0 0 Control 2 0 0 0 Control group 3 3 0 0 Experiment group 1 7 6 5 Experiment group 2 7.5 5.5 4

As a result, although the antimicrobial effect was small in the control group including the simple red ginseng water extract, the experimental groups 1 and 2, which are fractions of the present invention, were found to have excellent antimicrobial effects at low concentrations as well as high concentrations. In addition, experimental groups 1 and 2 did not show a significant difference in antimicrobial activity. Therefore, the fraction of the present invention can be used as an active ingredient in a cosmetic composition that requires an antimicrobial effect against acne bacteria.

< Example  2> whitening efficacy test

B16 / F1 melanoma cells (ATCC, USA) were grown up to 10 passages, and 5 × 10 ⁴ aliquots were added to 6-well plates, followed by 10% bovine serum in DMEM medium, followed by incubation at 37 ° C. and 5% CO ₂ incubator. It was. After 24 hours, the control and experimental groups were treated with 0.063 and 0.032 mg / ml, respectively, and the positive control group was treated with 50 ppm of p-coumaric acid. After 72 hours, melanoma cells were physically detached and dissolved with melanin at 70 ° C. using 1N NaOH containing DMSO, and then absorbance was measured at 405 nm. As the sample, Experimental Group 1 and Experimental Group 2, which are HPLC fractions obtained in Production Example <1-3>, were used, and representatively, only the results of Experimental Group 1 are shown. Control groups 1-3 were used as comparative examples of the experimental group.

As a result of confirming the melanin synthesis inhibitory effect of each sample, a simple butanol fraction (control group 1), a water layer generated when butanol fraction (control group 2), HPLC after butanol fraction (developing solvent: H ₂ 0: Acetonitrile = 68: 32 ~ 72 : 28) fraction (control 3) did not appear to effectively inhibit melanin pigment production. On the other hand, after the butanol fraction fraction of the present invention HPLC _{(eluent: H 2 0: Acetonitrile = 10:90} ) the purified fractions in (experiment 1) or column fractions after purification HPLC (eluent: H ₂ 0: Acetonitrile = 10:90) fraction (Experimental Group 2) significantly inhibited the production of melanin pigment compared to other fractions, and when treated with 0.063mg / ml concentration, the positive control p-coumaric acid was 0.250mg / ml concentration. It showed almost the same inhibitory effect on melanin production as treated. Experimental groups 1 and 2 also showed no significant difference in melanin inhibitory effect.

From the above results, the fraction of the present invention inhibits the production of melanin pigments compared to the simple fraction, and inhibits the production of the same melanin pigment at a lower concentration than the positive control group, and thus can be used as an active ingredient in a cosmetic composition requiring a whitening effect. It can be seen that.

Example 1 and Example 2:

HPLC fractions (Experimental group 1, 2) of Preparation Example 1 of the present invention shows a high antibacterial effect against acne bacteria, and also excellent whitening effect can be usefully used as a raw material of cosmetics, household goods.

Preparation Example 2 Preparation of Cosmetic Composition

<2-1> Preparation of Essence

Using the HPLC fraction (Experimental Group 1) of Preparation Example <1-3>, an essence was prepared according to the content (parts by weight) shown in Table 2 below.

Furtherance Content (parts by weight) Triethanolamine 0.25 Carboxy Vinyl Polymer 0.22 glycerin 4 Butylene glycol 2 HPLC fractions of Preparation Example <1-3> (Experimental Group 1) 1.5 Beeswax 0.5 Cetostearyl alcohol One Glyceryl Monostearate One Squalene 4 Purified water Quantity

<2-2> Preparation of Flexible Cosmetic Water

Softening water containing the HPLC fraction (Experimental group 2) of Preparation Example <1-3> as an active ingredient was prepared as shown in Table 3 below.

Raw material Content (parts by weight) 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 PEG-40
Hydrogenated Castor Oil 1.00 ethanol 30.00 HPLC fractions of Preparation Example <1-3> (Experimental Group 2) 0.5 coloring agent a very small amount Flavor a very small amount Purified water Remaining amount

<2-3> Preparation of nutrition cream

Nutritional cream containing the HPLC fraction of Experimental Example <1-3> (Experimental Group 1) as an active ingredient was prepared as shown in Table 4 below.

Raw material Content (parts by weight) 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Magnesium Aluminum Silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Lipophilic glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalene 3.8 HPLC fractions of Preparation Example <1-3> (Experimental Group 1) 1.5 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl Acetate Quantity Triethanolamine Quantity Flavor Quantity Purified water Remaining amount

<2-4> Preparation of Lotion

A lotion containing the HPLC fraction of Experimental Example <1-3> (Experimental Group 2) as an active ingredient was prepared as shown in Table 5 below.

Raw material Content (parts by weight) Cetostearyl alcohol 1.6 Stearic acid 1.4 Lipophilic Monostearic Acid Glycerin 1.8 PEG-100 Stearate 2.6 Sesquioleate Sorbate 0.6 Squalene 4.8 Macadamia oil 2 Jojoba oil 2 Tocopherol Acetate 0.4 Methylpolysiloxane 0.2 Tocopherol Acetate 0.4 1,3-butylene glycol 4 Xanthan Gum 0.1 glycerin 4 d-pandenol 0.15 HPLC fractions of Preparation Example <1-3> (Experimental Group 2) 1.0 Allantoin 0.1 Carbomer (2% aq.Sol) 4 Triethanolamine 0.15 ethanol 3 Purified water Quantity

Claims (12)

Purifying the column purified fraction obtained by fractionating red ginseng water extract with butanol and separating only butanol layer, or column purified fraction obtained by column purification fraction of red ginseng water extract;
As a method for preparing an HPLC fraction of red ginseng extract comprising,
As the HPLC proceeds, it develops sequentially while giving a concentration gradient with a developing solvent (water: acetonitrile = 5 to 35:65 to 95) mixed with water and acetonitrile.
The HPLC fraction is a method for producing an HPLC fraction of the red ginseng extract that recovers the peak portion that appears when using a mixed solvent of water and acetonitrile (water: acetonitrile = 10 ~ 20: 80 ~ 90).
The method according to claim 1,
When the red ginseng water extract is fractionated into butanol, the butanol is used in an amount of 0.1 to 25 parts by weight based on 10 parts by weight of the red ginseng water extract.
The method according to claim 1,
The butanol is a manufacturing method of the butanol aqueous solution.
The method according to claim 3,
The butanol aqueous solution is a saturated butanol.
The method according to claim 1,
The column is a silica gel or an aromatic synthetic resin.
delete delete delete The method according to claim 1,
The HPLC fractionation step is to recover the peak portion appearing when using a developing solvent (water: acetonitrile = 10:90) mixed with water and acetonitrile.
HPLC fractions of red ginseng extracts prepared by the process of any one of claims 1-5 and 9.
Antimicrobial cosmetic composition comprising an HPLC fraction of the red ginseng extract of claim 10.
Whitening cosmetic composition comprising an HPLC fraction of the red ginseng extract of claim 10.

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