JP3987733B2 - Fibroblast activator and skin external preparation containing the same - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
  The present invention relates to a novel fibroblast activator effective for preventing skin aging and preventing or improving rough skin, and a skin external preparation containing the fibroblast activator.
[0002]
[Prior art]
  Skin aging is caused by internal factors such as inactivation of cell growth / differentiation associated with aging, decreased hormone secretion, and damage to cells / tissues or inflammation based on active oxygen induced by exposure to sunlight (ultraviolet rays). This is a phenomenon that occurs when external factors such as occurrence are intricately intertwined.
  Of these, skin aging based on internal factors is mainly due to a decrease in the proliferation ability and activity of dermal fibroblasts, and the extracellular matrix such as collagen, fibronectin, and mucopolysaccharide produced by the cells. The amount of ingredients decreases and skin tissue loses its flexibility and elasticity, and morphological and physiological changes appear as wrinkles, sagging and rough skin.
[0003]
  In order to prevent or improve skin aging based on this decrease in cell activity and maintain the skin in a youthful and firm state, α-hydroxycarboxylic acids, vitamins, hormones, placenta have been conventionally used as cell activation components. Although it has been proposed that an extract or the like is blended in an external preparation for skin, none of them is necessarily sufficient in terms of effectiveness or safety, and it contains a cell activation component with higher utility as well as this. There is a need for a topical skin preparation.
[0004]
[Problems to be solved by the invention]
  In view of the problems of the prior art as described above, the present inventors conducted extensive research to find a cell activation component that is excellent in both effectiveness and safety, and as a result, an extract derived from a plant belonging to the Brassica family Brassica. Has a strong activation effect on fibroblasts, and has a high safety based on being derived from a natural product, as well as a new cell activation component having both effectiveness and safety, and this component as an active ingredient It is possible to provide an external preparation for preventing skin aging, preventing or improving rough skin, and when using ascorbic acid or a derivative thereof in combination with the plant extract, surprisingly their synergistic effects The cell activation effect is remarkably enhanced by this, and in combination with the good safety of ascorbic acid and its derivatives, a cell activation component and an external preparation for skin that are more useful are provided. Become known the Rukoto, and have completed the present invention.
[Means for Solving the Problems]
[0005]
  That is, the present invention firstly comprises an extract of Brassica hirta of the Brassicaceae Brassica plant and 2-O-α-D-glucopyranosyl-L-ascorbic acid of ascorbic acids.In a solid content weight ratio of 2: 1 to 1: 100A fibroblast activator.
  The present invention secondly relates to a skin external preparation containing the fibroblast activator of the first invention.
[0006]
  Hereinafter, the present invention will be described in detail.
AExamples of the plant belonging to the genus Brassica are Brassica hirta, Brassica juncea, Blackica nigra, Brassica ropa, and the like.However, in the present invention,Among them, white lion from the viewpoint of the activation effect of the extract on fibroblasts( Brassica hirta ) Is used.
[0007]
  the abovePreparation of the extract of Brassica Brassica plant is an appropriate part such as seeds, leaves, stems, roots, whole plants of the plant, preferably using seeds as an extraction raw material, and if necessary, pre-washing, drying, It is performed by chopping or pulverizing and bringing into contact with an extraction solvent by an appropriate means such as dipping or countercurrent extraction.
[0008]
  As an extraction solvent, water; lower alcohols such as methanol, ethanol, and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol, and octyldodecanol; many solvents such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin Monohydric alcohols; esters such as ethyl acetate, butyl acetate and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene and chloroform; These may be used alone or in admixture of two or more.
  Among these extraction solvents, a kind selected from water, lower alcohols and polyhydric alcohols from the viewpoint of the cell activation effect of the extract obtained, and from the point of wide applicability to external preparations for skin Alternatively, it is particularly preferable to use two or more kinds of mixed solvents, in particular, water or a mixed solvent of water and any of ethyl alcohol, glycerin, and 1,3-butylene glycol.
[0009]
  The mixing ratio in the case of using a mixed solvent is, for example, 1: 1 to 25: 1 by volume ratio (hereinafter the same) if the mixed solvent is water and ethyl alcohol, and 1: if the mixed solvent is water and glycerin. In the case of a mixed solvent of 1 to 15: 1 or water and 1,3-butylene glycol, it is preferably in the range of 1: 1 to 15: 1.
[0010]
  In preparing the extract of the present invention, the pH of the extract is preferably maintained in the range of 5 to 9. In this sense, if necessary, the extraction solvent may be sodium hydroxide, sodium carbonate, water. You may mix | blend alkaline adjusters, such as potassium oxide, acidic adjusters, such as a citric acid, hydrochloric acid, phosphoric acid, and a sulfuric acid, and you may adjust so that it may become desired pH.
[0011]
  Extraction conditions such as extraction temperature and time vary depending on the type of solvent used, the extraction site of the plant and the degree of shredding, etc. In the case of room temperature extraction, the extraction time is preferably about 10 minutes to 1 day, particularly about 30 minutes to 2 hours.
[0012]
  The extract solution obtained here is generally adjusted to a pH of 4 to 8, and may be added to the skin external preparation as it is or as an appropriate concentration by vacuum concentration or the like. The powder may be blended by pulverization according to a conventional method such as a method or a freeze-drying method.
[0013]
  The extract of Brassica genus plant of the present invention prepared as described above has a strong activation action on fibroblasts as shown in test examples later, and is biosafety such as low skin irritation. It is excellent and useful as a fibroblast activator.
  Therefore, even if the extract is used alone, a skin external preparation that is effective and effective in preventing skin aging and preventing / improving rough skin is provided. When using ascorbic acids belonging to a highly safe group among the existing skin external preparation ingredients in combination with plant extracts, the safety of Brassica plant extracts is not impaired by such combinations. Of course, surprisingly, the synergistic action of the combined components significantly enhances the fibroblast activation action, which is inferior to that of a single system in terms of safety, and is even more effective. It has become clear that it is possible to provide an improved fibroblast activator and a skin external preparation containing the same.
[0014]
  Here, as ascorbic acid effective in combination with Brassica genus plant extract, as L-ascorbic acid; and L-ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium salt, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium phosphate phosphate, sodium L-ascorbate-2-sulfate, magnesium L-ascorbate-2-sulfate; L-ascorbic acid-2-glucoside (2-O-α-D Ascorbic acid sugar derivatives such as glucopyranosyl-L-ascorbic acid) and L-ascorbic acid-5-glucoside (5-O-α-D-glucopyranosyl-L-ascorbic acid); The groups are hexanoyl, octanoyl, decano L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate and the like, and L-ascorbic acid tetrafatty acid esters, etc.ButAmong these ascorbic acids, from the viewpoint of the effect of enhancing cell activation when used in combination with Brassica plant extracts,In the present inventionL-ascorbic acid-2-glucosideIs used.
[0015]
  When the extract of Brassica plant and ascorbic acid are used in combination as a fibroblast activator, the amount ratio of these two components is the solid weight ratio2: Within the range of 1-1: 100.
[0016]
  Brassica plant extractionThings andThe external preparation for skin of the present invention containing ascorbic acids can be used as any of cosmetics, quasi drugs, medicines, etc., and the application site covers all skin including the scalp, and there is no particular limitation . Therefore, the dosage forms include, for example, emulsions, creams, lotions, essences, ointments, packs, haps, skin cleansers (soaps, etc.), facial cleansers, hair tonics, shampoos, rinses, treatments, and various makeup cosmetics. It can be various, such as bath salt.
[0017]
  The blending amount of Brassica plant extract and ascorbic acid in the external preparation for skin of the present invention varies depending on the use and application site of the external preparation for skin.,Within the above-mentioned range of the ratio of both components, the Brassica plant extract is 0.001 to 0.8% by weight, particularly 0.01 to 0.5% by weight, and the ascorbic acid is 0.01 to 10% by weight, particularly 0.1 to 5% by weight. It is better to mix to the extent.
[0018]
  In the external preparation for skin of the present invention, the above-mentioned Brassica plant extractionThings andIn addition to ascorbic acids, components commonly used in external preparations for skin, such as oily components, surfactants, moisturizers, thickeners, antiseptics / bactericides, powder components, UV absorbers, antioxidants, pigments, fragrances In addition, physiologically active ingredients and the like can be appropriately blended as necessary.
[0019]
  Here, examples of the oil component include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, plant-derived squalane. Plant-derived oils and fats; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax and lanolin; hydrocarbons such as liquid paraffin, petrolatum, paraffin wax and squalane; myristin Fatty acids such as acid, palmitic acid, stearic acid, oleic acid and isostearic acid; higher alcohols such as lauryl alcohol, cetanol and stearyl alcohol; isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, higher fatty acids Ok Rudodeshiru synthetic esters such as (stearic acid octyldodecyl) and synthetic triglycerides, and the like.
[0020]
  Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, Quaternary ammonium salt, primary to tertiary fatty amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.
  Moreover, lecithin and its derivatives, lactic acid bacteria fermented rice, etc. can also be mix | blended as an emulsifier thru | or an emulsification adjuvant.
[0021]
  Examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and sugars such as trehalose, lactic acid bacteria fermented rice, hyaluronic acid and Examples thereof include lactic acid, urea, higher fatty acid octyldodecyl, various amino acids, and derivatives thereof.
[0022]
  Examples of thickeners include, for example, brown algae such as alginic acid, agar, carrageenan and fucoidan, green algae or red algae-derived components, polysaccharides such as pectin, locust bean gum and aloe polysaccharide, gums such as xanthan gum, tragacanth gum and guar gum, carboxy Examples include cellulose derivatives such as methyl cellulose and hydroxyethyl cellulose, synthetic polymers such as polyvinyl alcohol, polyvinyl pyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and derivatives thereof, polyglutamic acid and derivatives thereof, and the like.
[0023]
  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzal chloride Luconium, salicylic acid, ethanol, undecylenic acid, phenols, jamal (imidazodenyl urea), 1,2-pentanediol, and the like.
[0024]
  Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, silk powder, cereals (rice, Wheat, corn, millet, etc.) and beans (soybeans, red beans, etc.) powder.
[0025]
  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate and the like.
[0026]
  Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and derivatives thereof.
[0027]
  Examples of physiologically active ingredients include whitening ingredients such as kojic acid and its derivatives, arbutin and its derivatives, ellagic acid and its derivatives, resorcinol derivatives, Sakuhakuhi extract, yukinoshita extract, rice bran extract, rice bran extract hydrolysate, Examples include seaweed extracts such as Anaaaosa, seaweed extracts such as sea cucumber, linoleic acid and derivatives or processed products thereof (for example, liposomal linoleic acid), 2,5-dihydroxybenzoic acid derivatives, and the like.
  In the present invention, when a Brassica plant extract and ascorbic acid are used in combination, it can be said that the ascorbic acid used here functions effectively as a whitening component in addition to the cell activation component. Not too long.
[0028]
  Here, examples of the kojic acid derivative include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, kojic acid ethers, and kojic acid glucoside. Examples of sugar derivatives include resorcinol derivatives such as 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid and 2-acetoxy-5. -Hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid and the like.
[0029]
  Moreover, as long as the effectiveness of the fibroblast activator of the present invention is not impaired, other components for preventing skin aging and improving rough skin (beautifying skin) may be used in combination, such as collagen, nicotinic acid and its components. Derivatives (nicotinic acid amide, benzyl nicotinate, etc.), glycyrrhizic acid and its derivatives (dipotassium salt, etc.), vitamin A and its derivatives, vitamin E and its derivatives (vitamin E nicotinate, vitamin E linoleate, etc.), allantoin, lactic acid, citrate Herbal extracts such as acids, α-hydroxy acids such as α-hydroxyoctanoic acid, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, gentian extract, licorice extract, pearl barley extract, chamomile extract, carrot extract, aloe extract , Rice extract hydrolyzate, rice fermentation , There is a Mitsuishikonbu extract or the like.
[0030]
  Next, the example(Preparation example of white coconut extract),Production example (preparation example of fibroblast activator), Formulation example (skin external preparationPrescriptionExample) and test examplesAs well as reference examples and reference formulation examplesHowever, the present invention is not limited to these examples. In the following, all parts are parts by weight, and all% are% by weight.
[0031]
  Example 1.
  Purified water (1000 g) was mixed with 50 g of white coconut seed pulverized product, followed by extraction at 40 ° C. for 1 hour, followed by filtration to obtain 820 g of a pale yellow transparent white coconut extract solution (solid content concentration 0.96%).
[0032]
  Example 2.
  50 g of white coconut seed pulverized product was mixed with 1000 g of a 30% 1,3-butylene glycol aqueous solution, extracted at 40 ° C. for 1 hour, and then filtered to obtain 800 g of a pale yellow transparent white coconut extract solution ( Solid concentration 0.94%).
[0033]
  Example 3.
  50 g of white coconut seed pulverized product was mixed with 1000 g of a 20% ethanol aqueous solution, extracted at 40 ° C. for 1 hour and filtered to obtain 880 g of a pale yellow transparent white coconut extract solution (solid content concentration 0.91%) ).
[0034]
  referenceExample1.
  Purified water (1000 g) was mixed with 50 g of crushed coconut seeds, followed by extraction at 40 ° C. for 1 hour, followed by filtration to obtain 850 g of a pale yellow transparent coconut extract solution (solid content concentration: 0.94%).
[0035]
  referenceExample2.
  Purified water (1000 g) was mixed with 50 g of black coconut seed pulverized product, followed by extraction at 40 ° C. for 1 hour, followed by filtration to obtain 820 g of a pale yellow transparent black coconut extract solution (solid content concentration: 1.01%).
[0036]
  Example4).
  500 g of the white coconut extract solution prepared in the same manner as in Example 1 was freeze-dried and then pulverized to obtain 4.7 g of a slightly yellow white coconut extract powder.
[0037]
  ManufacturingExample1. Preparation of fibroblast activator (1)
  20 g of L-ascorbic acid-2-glucoside was dissolved in 80 g of the white coconut extract solution of Example 1 to obtain a mixed solution containing the white coconut extract and L-ascorbic acid-2-glucoside.
[0038]
  ManufacturingExample2. Preparation of fibroblast activator (2)
  Purified water 30 g was diluted with 50 g of the white coconut extract solution of Example 1, and 20 g of L-ascorbic acid-2-glucoside was dissolved therein to contain the white coconut extract and L-ascorbic acid-2-glucoside. A mixed solution was obtained.
[0039]
  Test Example 1 Fibroblast activation
  The white coconut extract solution obtained in Example 1 as a Brassica genus plant extract was also obtained from ascorbic acid.LikeThen, using each of L-ascorbic acid-2-glucoside, the fibroblast activation action was examined for the case of Brassica genus plant extract alone and in the case of the combination of Brassica genus plant extract and ascorbic acids.
[0040]
  [Test method]
  Human dermis-derived fibroblast NB1RGB [Lot.011201 (9)] was placed in a 96-well microplate containing 1.0% FCS-containing Eagle's minimum essential medium at 1 × 10⁴ Individual / hole seeding, 5.0% CO₂After pre-culturing at 37 ° C. for 1 day in the atmosphere of the above, the extract solution of Example 1 is added to the medium to a concentration of 2.5% or 5.0%, or the extract solution of Example 1 and L Add ascorbic acid-2-glucoside to a concentration of 2.5% and 0.5 mM or 5.0% and 0.5 mM, respectively, and add 5.0% CO₂And further cultured at 37 ° C. for 3 days. Next, after removing the medium and adding 0.03% MTT and holding at 37 ° C. for 1 hour, the produced formazan was extracted with acidic isopropanol and wavelength was measured using a microplate reader (Model 450, manufactured by Bio-Rad). MTT values were measured at 570-630 nm.
  For comparison, the same test was performed for the case where L-ascorbic acid-2-glucoside was added alone and for the case where no sample was added (blank).
[0041]
  [result]
  The results are shown in FIG. In FIG. 1, AsG means L-ascorbic acid-2-glucoside.
  As shown in FIG. 1, the extract of the white cocoon of Brassicaceae Brassica plant (the extract solution of Example 1) has an action of enhancing the MTT uptake activity of fibroblasts and activating the cells. Moreover, when L-ascorbic acid-2-glucoside is used in combination with the white coconut extract, the fibroblast activation effect is further enhanced by the synergistic action of these two components.
[0042]
  Test Example 2 Skin irritation
[sample]
  Each of the following components was kneaded with JP NP hydrophilic petrolatum to a concentration of 5% and used as a sample.
  (1) Extract solution of Example 1
  (2)ManufacturingExample1Mixed solution
[0043]
[Test method]
  Five adult males aged 20 to 50 years were used as subjects, and the inner surface of each upper arm was wiped with ethanol to remove sebum. A coating applied to 0.2 g was attached. After 24 hours, the fin chamber was removed, and the degree of skin irritation was determined by the method and criteria described below.
[Judgment]
  After 1 hour, 24 hours and 48 hours after removing the patch, the state of erythema and edema at the applied site was visually determined based on the following “skin irritation criteria by dreze method”, and the average value of 5 subjects was calculated. Asked.
    (Erythema)
    Score     Skin condition
      0: No erythema
      1: Extremely mild erythema
      2: Clear erythema
      3: Moderate to strong erythema
      4: Light crust formation on deep crimson erythema
    (edema)
    Score     Skin condition
      0: No edema
      1: Extremely mild edema
      2: Clear edema (distinguishable from surroundings)
      3: Moderate edema (swelling of 1 mm or more)
      4: Strong edema (further spread around)
[0044]
  [result]
  The results are shown in Table 1.
[Table 1]
[0045]
  As is clear from the results in Table 1, the white coconut extract or the fibroblast activator comprising the extract and L-ascorbic acid-2-glucoside has almost no skin irritation and is extremely excellent in safety.
[0046]
    referenceFormulation Example 1 cream
  [Component A] part
  Liquid paraffin 5.0
  Hexalan (Note 1) 4.0
  Paraffin 5.0
  Glyceryl monostearate 2.0
  Polyoxyethylene (20) sorbitan monostearate 6.0
  Butylparaben 0.1
       (Note 1) Technoble Co., Ltd. glyceryl trioctanoate
[B component]
  Extract solution of Example 1 10.0
  Glycerin 5.0
  Methylparaben 0.1
  Moiston C (Note 2) 1.0
  Amount of purified water totaling 100 parts
       (Note 2) NMF component manufactured by Technoble Co., Ltd.
[C component]
  Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After this was cooled to 50 ° C., component C was added and further stirred and mixed to obtain a cream.
[0047]
  referenceFormulation Example 2 Latex
[Component A] part
  Liquid paraffin 6.0
  Hexalan 4.0
  Jojoba oil 1.0
  Polyoxyethylene (20) sorbitan monostearate 2.0
  Soy lecithin 1.5
  Methylparaben 0.15
  Ethylparaben 0.03
[B component]
  Extract solution of Example 1 10.0
  Glycerin 3.0
  1,3-butylene glycol 2.0
  Carboxymethylcellulose 0.3
  Sodium hyaluronate 0.01
  Amount of purified water totaling 100 parts
[C component]
  Perfume
  The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. This
After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.
[0048]
  Formulation example1. lotion
[Ingredient] part
  ManufacturingExample1A mixed solution of 10.0
  Ethanol 10.0
  Glycerin 3.0
  1,3-butylene glycol 2.0
  Methylparaben 0.2
  Citric acid 0.1
  Sodium citrate 0.3
  Carboxyvinyl polymer 0.1
  Perfume
  Potassium hydroxide appropriate amount
  Amount of purified water totaling 100 parts
  The above ingredients were mixed to obtain a lotion.
[0049]
  Formulation example2. Lotion
[Component A] part
  Olive oil 1.0
  Polyoxyethylene (5.5) cetyl ether 0.5
  Butylparaben 0.1
[B component]
  ManufacturingExample2A mixed solution of 10.0
  Ethanol 5.0
  Glycerin 5.0
  1,3-butylene glycol 5.0
  Methylparaben 0.1
  Potassium hydroxide appropriate amount
  Amount of purified water totaling 100 parts
[C component]
  Perfume
  After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes.
  After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.
[0050]
  Formulation example3. cream
[Component A] part
  Liquid paraffin 5.0
  Hexalan 4.0
  Paraffin 5.0
  Glyceryl monostearate 2.0
  Polyoxyethylene (20) sorbitan monostearate 6.0
  Butylparaben 0.1
[B component]
  Extract solution of Example 1 10.0
  L-ascorbic acid-2-glucoside 2.0
  Glycerin 5.0
  Methylparaben 0.1
  Moiston C 1.0
  Potassium hydroxide appropriate amount
  Amount of purified water totaling 100 parts
[C component]
  Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After this was cooled to 50 ° C., component C was added and further stirred and mixed to obtain a cream.
[0051]
  referenceFormulation example3. cream
  Formulation example3In addition to using 2.0 parts of L-ascorbic acid-2-glucoside in place of 2.0 parts of L-ascorbic acid-2-glucoside, a prescription example is used.3In the same manner, a cream was obtained.
[0052]
  referenceFormulation example4. cream
  Formulation example3In addition to using 2.0 parts of L-ascorbic acid-2-phosphate in place of 2.0 parts of L-ascorbic acid-2-glucoside, a prescription example is used.3In the same manner, a cream was obtained.
[0053]
  Formulation example4. Latex
[Component A] part
  Liquid paraffin 6.0
  Hexalan 4.0
  Jojoba oil 1.0
  Polyoxyethylene (20) sorbitan monostearate 2.0
  Soy lecithin 1.5
  Methylparaben 0.15
  Ethylparaben 0.03
[B component]
  Extract solution of Example 2 10.0
  L-ascorbic acid-2-glucoside 2.0
  Glycerin 3.0
  1,3-butylene glycol 2.0
  Carboxymethylcellulose 0.3
  Sodium hyaluronate 0.01
  Potassium hydroxide appropriate amount
  Amount of purified water totaling 100 parts
[C component]
  Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.
[0054]
  Formulation example5. lotion
[Ingredient] part
  Extract solution of Example 1 10.0
  L-ascorbic acid-2-glucoside 2.0
  Ethanol 10.0
  Glycerin 3.0
  1,3-butylene glycol 2.0
  Methylparaben 0.2
  Citric acid 0.1
  Sodium citrate 0.3
  Sodium alginate 0.1
  Potassium hydroxide appropriate amount
  Perfume
  Amount of purified water totaling 100 parts
  The above ingredients were mixed to obtain a lotion.
[0055]
  referenceFormulation example5. Liquid foundation
  [Component A] part
  Stearic acid 2.4
  Propylene glycol monostearate 2.0
  Cetostearyl alcohol 0.2
  Liquid lanolin 2.0
  Liquid paraffin 3.0
  Isopropyl myristate 8.5
  Propylparaben 0.05
  [B component]
  Extract solution of Example 2 5.0
  Sodium carboxymethylcellulose 0.2
  Bentonite 0.5
  Propylene glycol 4.0
  Potassium hydroxide 1.1
  Methylparaben 0.1
  Amount of purified water totaling 100 parts
  [C component]
  Titanium oxide 8.0
  Talc 4.0
  Coloring pigment appropriate amount
  The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.
[0056]
  PrescriptionExample6. Cream foundation
[Component A] part
    Stearic acid 5.0
    Cetanol 2.0
    Glyceryl monostearate 3.0
    Liquid paraffin 5.0
    Squalane 3.0
    Isopropyl myristate 8.0
    Polyoxyethylene (20) glyceryl monostearate 2.0
    Propylparaben 0.1
[B component]
    Extract solution of Example 1 5.0
    L-ascorbic acid-2-glucoside 2.0
    Sorbitol 3.0
    1,3-butylene glycol 5.0
    Potassium hydroxide 1.5
    Methylparaben 0.1
    Amount of purified water totaling 100 parts
[C component]
    Titanium oxide 8.0
    Talc 2.0
    Kaolin 5.0
    Bentonite 1.0
    Coloring pigment appropriate amount
[D component]
    Fragrance 0.3
  Component C was mixed and pulverized with a pulverizer. The component B was mixed, and the pulverized component C was added thereto and uniformly dispersed in a colloid mill. The components A and B and C dispersed uniformly were each heated to 80 ° C., and then the components A were added to the components B and C while stirring, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, D component was added and stirred and mixed, and also it cooled to 30 degrees C or less, stirring, and obtained the cream foundation.
[0057]
  referenceFormulation example6. Hair nick
[Component A] part
    Ethanol 60.0
    l-Menthol 0.5
    Fragrance 0.1
    Methylparaben 0.1
[B component]
    Glycerin 2.0
    1,3-butylene glycol 2.0
    Extract solution of Example 3 10.0
    Amount of purified water totaling 100 parts
  The above A component and B component were each dissolved at room temperature, and then the B component was added to the A component with stirring and dissolved to obtain a hair technic.
[0058]
  Reference prescriptionExample7. Hair Treatment
  [Ingredient] part
  Stearyltrimethylammonium chloride 6.0
  Polyvinylpyrrolidone 4.0
  Glycerin 1.0
  Ethylparaben 0.1
  Extract solution of Example 2 5.0
  Amount of purified water totaling 100 parts
  The above ingredients were heated to 80 ° C. and then mixed and stirred to obtain a hair treatment.
  This product was also suitable as a hair pack.
[0059]
  referenceFormulation example8. Hair shampoo
[Component A] part
    N-coconut oil fatty acid methyl taurine sodium 10.0
    Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
    Lauryldimethylaminoacetic acid betaine 10.0
    Palm oil fatty acid diethanolamide 4.0
    Methylparaben 0.1
[B component]
    Citric acid 0.1
    Extract solution of Example 2 5.0
    1,3-butylene glycol 2.0
    Amount of purified water totaling 100 parts
  After each component A and component B was heated to 80 ° C. and dissolved uniformly, component B was added to component A, and stirring was continued to cool to room temperature to obtain a hair shampoo.
[0060]
  referenceFormulation example9. Hair rinse
[Component A] part
    Polyoxyethylene (10) hydrogenated castor oil 1.0
    Distearyldimethylammonium chloride 1.5
    Stearyltrimethylammonium chloride 2.0
    Glyceryl 2-ethylhexanoate 1.0
    Cetanol 3.2
    Stearyl alcohol 1.0
    Methylparaben 0.1
[B component]
    Extract solution of Example 2 5.0
    1,3-butylene glycol 5.0
    Amount of purified water totaling 100 parts
  After each component A and component B was heated to 80 ° C. and uniformly dissolved, component B was added to component A, and stirring was continued to cool to room temperature to obtain a hair rinse.
[0061]
  referenceFormulation exampleTen. Body shampoo
[Component A] part
    N-lauroylmethylalanine sodium 25.0
    Palm oil fatty acid potassium liquid (40%) 26.0
    Palm oil fatty acid diethanolamide 3.0
    Methylparaben 0.1
[B component]
    Extract solution of Example 2 10.0
    1,3-butylene glycol 2.0
    Amount of purified water totaling 100 parts
  After each component A and component B was heated to 80 ° C. and uniformly dissolved, component B was added to component A, and stirring was continued to cool to room temperature to obtain a body shampoo.
[0062]
  Reference prescriptionExample11. Soap
[Component A] part
    Hardened castor oil 26.0
    Coconut oil 10.0
    Olive oil 4.0
[B component]
    Sodium hydroxide 6.0
    Sugar 10.0
    Glycerin 5.0
    Example4Extract powder of 0.5
    Amount of purified water totaling 100 parts
[C component]
    Ethanol 20.0
    Perfume
  The components A and B were each heated to 80 ° C. and dissolved uniformly, and then the component B was added to the component A to saponify. This was cooled to 50 ° C. with stirring, and component C was added. This was poured into a mold and cooled, and then dried at room temperature for several days. A sufficiently dried product was taken out from the mold to obtain soap.
[0063]
  referenceFormulation example12. Latex
  referenceIn the B component of Formulation Example 2, instead of the extract solution of Example 1referenceExample1Except using the extract solutionreferenceAn emulsion was obtained in the same manner as in Formulation Example 2.
[0064]
  referenceFormulation example13. Latex
  referenceIn the B component of Formulation Example 2, instead of the extract solution of Example 1referenceExample2Except using the extract solutionreferenceAn emulsion was obtained in the same manner as in Formulation Example 2.
[0065]
  referenceFormulation example14. Latex
[Component A] part
  Liquid paraffin 6.0
  Hexalan 4.0
  Jojoba oil 1.0
  Polyoxyethylene (20) sorbitan monostearate 2.0
  Soy lecithin 1.5
  Methylparaben 0.15
  Ethylparaben 0.03
[B component]
  Extract solution of Example 1 10.0
  Kojic acid 1.0
  Glycerin 3.0
  1,3-butylene glycol 2.0
  Carboxymethylcellulose 0.3
  Sodium hyaluronate 0.01
  Amount of purified water totaling 100 parts
[C component]
  Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.
[0066]
  referenceFormulation example15. Latex
  referenceFormulation example14In addition to using 1.0 part of arbutin in place of 1.0 part of kojic acid,referenceFormulation example14In the same manner, an emulsion was obtained.
[0067]
  referenceFormulation example16. Latex
  referenceFormulation example14In addition to using 1.0 part of kojic acid, 5.0 parts of rice extract hydrolyzate (Technoble Co., Ltd., trade name “Orizeno Noble”, solid content concentration 1.5%)referenceFormulation example14In the same manner, an emulsion was obtained.
[0068]
  referenceFormulation example17. Latex
  referenceFormulation example14Except that 1.0 part of γ-amino-β-hydroxybutyric acid is used in place of 1.0 part of kojic acidreferenceFormulation example14In the same manner, an emulsion was obtained.
[0069]
Test Example 3 Panel test
  About the cream containing the fibroblast activator of this invention, the effect with respect to skin was investigated by the panel test.
  [sample]
  (1)referenceCream of Formulation Example 1
  (2) Formulation example3Cream
  (3)referenceIn the B component of Formulation Example 1, except that purified water is used instead of the extract solution of Example 1.referenceCream obtained in the same manner as in Formulation Example 1 (control)
[0070]
  [Test method]
  Randomly extracted 40 women aged 18-50 were divided into two groups of 20 subjects each, and one groupreferenceFormulation example 1 cream and control cream, and the other group3Allocate cream and control cream, and apply the prescription example or control cream to the left and right cheeks twice a day (morning and evening) for 1 month, then improve wrinkles and improve skin The improvement effect on paste and gloss was evaluated based on the following evaluation criteria.
[0071]
  [Evaluation criteria]
    (Improvement effect on small wrinkles)
        A: almost unnoticeable
        B: It became pretty inconspicuous
        C: Slightly inconspicuous
        D: Unchanged
        E: On the contrary increased
    (Improvement effect on skin elasticity and gloss)
        A: Clearly improved
        B: Remarkably improved
        C: Slightly improved
        D: unchanged
        E: It got worse
[0072]
  [result]
  The results are shown in Table 2. In addition, the number of each evaluation column of AE of Table 2 shows the ratio (%) of the test subject who performed the said evaluation among 20 test subjects.
[0073]
[Table 2]
[0074]
  From the results of Table 2, a cream containing a Brassicaceae Brassica plant extract or a fibroblast activator comprising the extract and ascorbic acid (L-ascorbic acid-2-glucoside) has an excellent skin aging preventing effect, Having a rough skin improving effect, and those effects are more prominent particularly in creams containing a brassica family Brassica plant extract and a fibroblast activator comprising ascorbic acid (L-ascorbic acid-2-glucoside). It turns out that it is recognized.
【The invention's effect】
AThe extracts of Brassicaceae Brassica plants have a strong stimulating effect on dermal fibroblasts, and because they are derived from natural products, they have excellent biological safety, preventing skin aging and preventing / improving rough skin. It is useful as a fibroblast activator for a topical skin preparation. orAccording to the present inventionBy combining ascorbic acids with an extract of Brassica Brassica plant, a fibroblast activator that maintains high biological safety as it is and that has further improved effectiveness is provided.
  Therefore, according to the present invention,The above, wherein ascorbic acids are combined with an extract of Brassica BrassicaContains fibroblast activatorIsAn external preparation for skin that is effective for preventing skin aging and preventing or improving rough skin is provided.
[Brief description of the drawings]
FIG. 1 is a graph showing the MTT value of each sample of Test Example 1 (vertical axis: MTT value).

Claims (2)

The extract of Brassica hirta from the Brassicaceae family and the ascorbic acid 2-O-α-D-glucopyranosyl-L-ascorbic acid in a solid content weight ratio range of 2: 1 to 1: 100. A fibroblast activator comprising: A skin external preparation containing the fibroblast activator according to claim 1.