JP4769153B2 - Cosmetics - Google Patents

Description

  The present invention relates to a cosmetic that is effective for preventing skin aging and preventing or improving rough skin. More specifically, the present invention has both a collagen production promoting action in epidermal cells and a collagen production promoting action in dermal fibroblasts, and the synergistic effect of these two actions. Contains natural ingredients that are highly effective in preventing skin aging and preventing and improving rough skin, and is effective in maintaining or improving the skin in a healthy and youthful state. It is related with the cosmetics useful for use.

Skin aging is caused by internal factors such as inactivation of cell growth / differentiation associated with aging and a decrease in hormone secretion, cell / tissue damage by active oxygen induced by sunlight (ultraviolet rays), and extracellular matrix components. This is a phenomenon that is caused by complicated intertwining of external factors such as quantitative decrease in inflammation or inflammation. Among them, aging of the skin based on internal factors is due to inactivation of epidermal basal cells based on decreased activity of epidermal cells, particularly decreased synthesis of proteins constituting basement membranes such as type IV and type VII collagen and laminin, This is caused by a decrease in the barrier function, sebum secretion function, and water retention function of the skin stratum corneum. In addition, dermal fibroblast activity declines and proliferative ability, or dermal matrix components such as collagen, elastin, and hyaluronic acid decrease, and skin tissue loses flexibility and elasticity, which also causes skin aging. As morphological and physiological changes, symptoms such as wrinkles, tarmi and rough skin appear.
In order to prevent this skin aging and keep the skin healthy and youthful, the use of various active ingredients has been proposed and cosmetics containing these ingredients have been put on the market. For example, antioxidants such as vitamin C and vitamin E, anti-inflammatory agents such as glycyrrhizic acid, various ultraviolet absorbers, α-hydroxycarboxylic acid, placental extract, and cell activation such as γ-amino-β-hydroxybutyric acid Ingredients, extracellular matrix components such as collagen, elastin and hyaluronic acid, humectants such as urea, and the like.
However, since these ingredients generally only serve to prevent or improve some of the above-mentioned skin aging factors, depending on the conventional cosmetics containing these ingredients, the skin aging that is truly satisfactory At present, the prevention effect cannot be obtained. In addition, depending on the type of component, there are problems such as having skin irritation and skin sensitization, and there are problems with safety against living bodies, or cell activation and proliferation are not sufficient.

  In view of the above-mentioned problems seen in conventional cosmetics, the present inventors have combined and multifaceted action against various factors such as skin aging and rough skin, thereby preventing skin aging and rough skin. As a result of diligent research to find a new cosmetic compounding ingredient that shows high effectiveness in prevention and improvement, and is also sufficiently satisfactory in terms of safety to the skin, squeezed juice of water eggplant, and its extract, In addition to promoting the collagen production of epidermal cells and promoting collagen production of dermal fibroblasts, it is derived from vegetables that have been eaten for a long time, so it has excellent biological safety and meets the above objectives. As a result, the present invention has been completed.

Various proposals have heretofore been made for using plant components of the solanaceous genus (Solanum genus) as cosmetic ingredients.
For example, Japanese Patent Laid-Open No. 03-190809 discloses a skin external preparation containing an extract of eggplant that prevents or improves rough skin, and in particular, prevents or improves rough skin based on suppressing inflammation and excessive turnover by an active agent. JP-A-10-316530 discloses that an extract of a plant belonging to the genus Solanum belongs to the genus Solanum, which suppresses the production of melanin, and prevents and improves pigmentation, blotches, freckles, and liver spots after sunburn. JP 2000-290162 discloses that the skin is moist and moisturized by a cosmetic containing water derived from vegetables separated in the step of concentrating vegetable juice of vegetables such as eggplant. Japanese Patent Application Laid-Open No. 2001-131046 discloses a hydrogen peroxide scavenger composed of the essence of solanaceous plants and this. JP-A-2001-139420 has an effect of activating catalase in the essence of eggplant and is effective for use in eliminating hydrogen peroxide. JP-A-2001-226219 The publication discloses that a cosmetic composition containing eggplant steam distilled water has the effect of improving dry skin and imparting gloss and tension to the skin.

However, in these known documents, the use of water eggplant (Solanum Melongena; Egg plant cv. 'Mizu-nasu') according to the present invention as a plant of the genus Solanum (Solanum) is specifically described. There is no disclosure at all.
Further, as shown in Test Examples 1 and 2 later, the collagen production promoting effect of epidermis cells and the collagen production promoting effect of dermal fibroblasts possessed by eggplant squeezed juice or extract are especially water eggplants among eggplants (Solanum Melongena). (Solanum Melongena; Egg plant cv. Mizunasu), it is obvious that there is no description in the publicly known literature or suggesting this fact.
However, among those known documents, for example, as seen in Japanese Patent Application Laid-Open Nos. 2001-139420 and 2001-226219, the eggplant-derived component has a wrinkle-preventing effect based on hydrogen peroxide erasure and dry skin. There are those disclosed that there is an effect of preventing skin aging such as the effect of imparting gloss and tension by improvement, but as shown in the results of the monitor test described later, the general-purpose eggplant component used in the inventions of those known documents has an effect The anti-aging effect such as improvement of skin wrinkles and tension is far less than that of the component derived from water eggplant of the present invention. The reason why such a special effect can be obtained by the water eggplant according to the present invention is that, as described above, among the eggplants, the water eggplant particularly promotes the collagen production of both epidermal cells and dermal fibroblasts. It is speculated that the multifaceted anti-aging effect contributes greatly.
In any case, from the description of the above-mentioned publicly known literature, the squeezed juice of water eggplant and its extract used as an active ingredient in the present invention have the effect of remarkably accelerating collagen production of epidermal cells and collagen production of dermal fibroblasts. Through the synergistic effect of these two actions, the skin, basement membrane, and even the dermis are made healthy and healthy, which is far superior to the commonly used eggplant-derived ingredients, preventing skin aging and preventing rough skin. It is clear that it is impossible to predict the improvement effect.

Japanese Patent Laid-Open No. 03-190809 JP 10-316530 A JP 2000-290162 A JP 2001-131046 A JP 2001-139420 A JP 2001-226219 A

That is, this invention relates to the anti-aging cosmetic characterized by mix | blending the 1 type (s) or 2 or more types chosen from the squeezed juice and / or extract of water eggplant.
The present invention also relates to an agent for promoting collagen production of epidermal cells and dermal fibroblasts, which contains one or more selected from squeezed juice and / or extract of water eggplant as an active ingredient.
As used herein, “water eggplant” refers to varieties such as seven conventional varieties of eggplant varieties, such as coconut coconut jelly, Izumi coconut lion, and Quanzhou kinkawa mizuko (Osaka Agricultural Science Research Report 35: 34-39 (1999)). In particular, those cultivated in the Quanzhou area of Osaka are preferably used in the present invention.
In the present invention, the extract means a dry pulverized product of water eggplant, an extract or a solid component obtained by removing the solvent from the extract by concentration or the like.
In the present invention, the term cosmetic is used in a broad sense including so-called cosmetics and quasi-drugs.

Osaka Prefectural Agricultural and Forestry Research Center Research Report 35: 34-39 (1999)

Juice eggplant squeezed juice and / or extract have both collagen production promoting effects of both epidermal cells and dermal fibroblasts, and the cosmetic composition of the present invention has a synergistic anti-aging action based on a combination of these actions. It improves skin elasticity and firmness, prevents or ameliorates skin aging and unhealthy symptoms such as wrinkles and tarmi, and keeps the skin truly healthy and youthful.
Also, the squeezed juice or extract of water eggplant used as an active ingredient in the cosmetic composition of the present invention has no irritation to the skin, and therefore the cosmetic composition of the present invention is very excellent in biological safety.

Hereinafter, the present invention will be described in detail.
In the present invention, as the eggplant, seven types of conventional strains such as cocoon syrup, suizu mizuko, zumishu kinkawa coconut, and the like can be used.

In the cosmetic composition of the present invention, one or more of the above-described squeezed juice (fruit juice) and / or extract of the water eggplant is blended as an active ingredient.
As shown later in the test examples, the squeezed juice and extract of water eggplant have an action of remarkably accelerating their collagen production on both epidermal cells and dermal fibroblasts. It can be used as a collagen production promoter for fibroblasts.

The squeezed juice of water eggplant can be prepared by pressing the fruit as it is or by crushing the fruit and then pressing the crushed material.
The extract of water eggplant is obtained by drying and pulverizing the fruit of water eggplant to obtain a dried pulverized product, or by lyophilizing the juice or fruit of the water eggplant or, in some cases, the juice. Prepared by extracting dry powder with solvent.
Preparation of extract from water eggplant fruit is made by bringing the fruit into contact with an extraction solvent by appropriate means such as dipping method or countercurrent extraction method after pre-washing it with water, drying treatment etc. if necessary. Can be done by. A supercritical extraction method may also be used.

As an extraction solvent, water; lower alcohols such as methanol, ethanol, and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol, and octyldodecanol; many solvents such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin Monohydric alcohols; esters such as ethyl acetate, butyl acetate, methyl propionate and glyceryl trioctanoate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; carbonization such as n-hexane, toluene and chloroform Examples thereof include hydrogen-based solvents, which are used alone or in combination of two or more.
From the viewpoint of promoting collagen production in the epidermal cells and dermal fibroblasts of the extracted extract, in the present invention, among these solvents, those having high polarity are particularly preferably used. Furthermore, it is preferable to use one kind of single solvent or two or more kinds of mixed solvents selected from water, lower alcohols and polyhydric alcohols from the viewpoint that they can be widely applied to cosmetics. Is most preferably used alone.

  The mixing ratio in the case of using a mixed solvent is, for example, 1: 1 to 25: 1 by volume ratio (hereinafter the same) if the mixed solvent is water and ethyl alcohol, and 1: if the mixed solvent is water and glycerin. In the case of a mixed solvent of 1 to 20: 1, or water and 1,3-butylene glycol, it is preferably in the range of 1: 1 to 20: 1.

  In the preparation of the extract of the present invention, the pH of the extract is preferably maintained in the range of 5 to 9. In this sense, if necessary, the above extraction solvent may contain sodium hydroxide, sodium carbonate, potassium hydroxide. Alternatively, an alkaline adjusting agent such as arginine, an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid, sulfuric acid, or the like may be blended to adjust to a desired pH.

  Although extraction conditions such as extraction temperature and time vary depending on the type of solvent used, for example, in the case of the immersion method, the extraction temperature is generally in the range of 15 to 100 ° C., preferably 60 to 90 ° C., The extraction time is preferably about 0.1 to 24 hours, particularly about 0.5 to 3 hours.

When the above extraction process is completed, the liquid phase is separated by applying solid-liquid separation means such as filtration and centrifugation, and the pH is generally adjusted to 4 to 8 which is a preferable range as a cosmetic ingredient. In addition, it is blended in cosmetics as it is or after adjusting to an appropriate concentration by dilution or vacuum concentration. If necessary, this liquid phase may be powdered according to conventional methods such as spray-drying and freeze-drying and blended into cosmetics. In some cases, the extraction stock solution before applying solid-liquid separation means may be used as a cosmetic. It can also be blended.
Moreover, you may give this to the squeezed juice of water eggplant in said powdering process.

  It should be noted that the collagen production promoting action in the epidermis cells and dermal fibroblasts of the squeezed juice or extract of the water eggplant of the present invention is maintained as it is even if the fruit, juice or extract of mizusaki is subjected to a hydrolysis treatment. In addition, when the hydrolysis treatment is performed, there is an advantage that the operability at the time of extraction and the storage stability of the obtained extract are improved. Therefore, the hydrolysis treatment may be performed as necessary. As conditions for the hydrolysis treatment, conventional methods such as acid hydrolysis, alkali hydrolysis, enzyme hydrolysis and the like can be used.

  When enzyme hydrolysis is applied to the fruit, juice or extract of water eggplant, the enzymes include proteolytic enzymes such as actinase and papain, starch-degrading enzymes such as glucoamylase and α-amylase, cellulase, hemicellulase, pectinase, etc. 1 type or 2 or more types selected from lipolytic enzymes such as fibrinolytic enzymes and lipases are used, and in particular, 1 type or 2 or more types of enzymes selected from these 4 types of enzymes are used in combination. Is preferred.

In the case of using fruit or extract powder, the hydrolyzing treatment of water eggplant (fruit), juice or extract with those enzymes is preferably pre-crushed and fragmented, and then water or If a mixed solution of water and lower alcohol or polyhydric alcohol, preferably suspended or dissolved in water, and then using fruit juice or extract solution, adjust the concentration of the solution as it is or as necessary. After the treatment, the enzyme is added thereto, and the mixture is kept at around the optimum temperature of the enzyme for 1 to 24 hours.
In this case, when the solvent composition of the extract solution may hinder the enzyme reaction, the solvent is once distilled off, and the extract powder obtained here is preferably re-dissolved or dispersed in water to carry out the enzyme treatment. To do.
The amount of enzyme added is 0.01 to 10% by weight, preferably 0.1 to 1.0% in total, based on the solid content, when fruits are used as the object to be treated. Moreover, when using a squeezed liquid, an extract solution, or extract powder, it is 0.001-5 weight in total with respect to solid content similarly, Preferably it is the range of 0.01-0.5 weight%.

  When the above enzymatic hydrolysis treatment is completed, the treatment liquid is subjected to a heat treatment to deactivate the enzyme, and the liquid obtained here is collected as it is or by filtration, centrifugation, etc. Further, if necessary, it is subjected to pH adjustment, concentration adjustment, powdering treatment, etc. and then used as a cosmetic raw material.

  The squeezed juice of the eggplant (Solanum Melongena; Egg plant cv Mizu-nasu) of the present invention prepared as described above or an extract thereof, as shown in the test examples later, is used for epidermal cells and dermal fibroblasts. It has an effect of remarkably promoting collagen production, and is excellent in biological safety, such as being less irritating to the skin, and is useful as a formulation for cosmetics.

  Examples of cosmetics obtained by blending the squeezed juice and / or extract of the present invention or their hydrolyzed products thereof include, for example, basic cosmetics such as emulsions, creams, lotions, essences, packs, facial cleansers, and lipsticks. Makeup cosmetics such as foundations, liquid foundations, makeup press powders, hair shampoos, hair rinses, hair treatments, conditioners, hair dyes, hair conditioners and other hair cosmetics, facial cleansers, body shampoos, soaps, etc. Although cosmetics, a bath agent, etc. are mentioned, of course, it is not limited to these.

  The blended amount of the squeezed squeezed liquid and / or extract or enzyme hydrolyzed product thereof in the cosmetic of the present invention is generally 0.001 to 5% by weight as a solid content, for example, for basic cosmetics. , Preferably in the range of 0.01 to 3% by weight, for makeup cosmetics in general 0.001 to 5% by weight, preferably in the range of 0.01 to 2% by weight, and for hair cosmetics in general 0.001. It is generally in the range of 0.001 to 10% by weight, preferably in the range of 0.01 to 5% by weight.

The cosmetics of the present invention include components that are usually used in cosmetics, such as oily ingredients, surfactants, moisturizers, thickeners, antiseptics / sterilizers, in addition to the squeezed juice and / or extract of essential eggplant water. Add appropriate ingredients such as powders, powder components, UV absorbers, physiologically active ingredients (whitening ingredients, anti-aging / skin-care / wrinkle-preventing ingredients, etc.), anti-inflammatory agents, antioxidants, pigments, fragrances, etc. Can do.
In addition, a known collagen synthesis promoter can also be blended as long as the effectiveness and features of the squeezed squeezed juice and / or extract thereof of the present invention are not impaired.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty acid amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, etc. ON surfactant: quaternary ammonium salt, primary to tertiary fatty acid amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.
In addition, as emulsifiers or emulsifiers, stevia derivatives such as enzyme-treated stevia, lecithin and derivatives thereof, lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented cereals (wheat, beans, millet, etc.), juiziro (Rhamnaceae ) An extract or the like can also be blended.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, N- (3,4,5,6-tetrahydroxy-2- Oxohexyl) pyroglutamic acid, saccharides such as trehalose, lactic acid bacteria fermented rice, mucopolysaccharides (eg, hyaluronic acid and derivatives thereof, chondroitin and derivatives thereof, heparin and derivatives thereof), elastin and derivatives thereof, collagen and Derivatives, hydrolyzed silk protein, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, phytosterol, soybean phospholipid, cholesteryl isostearate, seaweed extract, seafood-derived collagen and its derivatives Various amino acids and their derivatives (such as trimethyl glycine, etc.), sphingolipids, Byakkyuu extract, soybean milk fermented liquor, natto extract, rice-derived extract and fermented, and the like.

  Examples of thickeners include, for example, brown algae such as alginic acid, agar, carrageenan, fucoidan, green algae or red algae-derived components, beech extract, pectin, locust bean gum, polysaccharides such as aloe polysaccharide, xanthan gum, tragacanth gum, guar gum, etc. Synthetic polymers such as gums, cellulose derivatives such as carboxymethylcellulose, hydroxyethylcellulose, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives, polyglutamic acid and its derivatives, Examples thereof include sugar compounds mainly composed of glucosyl trehalose and hydrolyzed hydrogenated starch.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Examples thereof include Luconium, Salicylic acid, Ethanol, Undecylenic acid, Phenols, Jamal (Imidazodenyl urea), 1,2-pentanediol, various essential oils, bark dry product, propolis extract, methylisothiazolinone.

  Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, 6- or 12-nylon powder, polyethylene powder. Silk powder, cellulosic powder, grains (rice, wheat, corn, millet, etc.) powder, beans (soybean, red beans, etc.) powder, and the like.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Examples of physiologically active ingredients include whitening ingredients such as t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone derivatives, ellagic acid and derivatives thereof, resorcinol derivatives, sucha akuhi extract, yukinoshita extract, rice bran Extract, rice bran extract hydrolyzate, lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented cereals (wheat, legumes, minor grains), white coconut hydrolyzed extract, murasakixikibu extract, lotus seed fermented product, party participation Extract, Pandanus amaryllifolius Roxb extract, Arcangelicia flava Merrilli extract, chamomile extract (trade name: Chamomile ET), ginger extract Japanese knotweed extract, Sahweed extract, licorice extract, dandelion extract, artea extract, genus shochu extract, saxifrage extract, jujube extract, peonies extract, suzuki extract, peach extract, citrus (mandarin orange, citron, Extract, etc.), green algae, red algae or brown algae seaweed extract, seaweed extract such as sea eel, linoleic acid and its derivatives or processed products (eg liposomal linoleic acid), 2,5-dihydroxy Benzoic acid derivatives, etc., as skin aging prevention, skin beautification and wrinkle prevention ingredients, animal and fish-derived collagen and derivatives thereof, elastin and derivatives thereof, nicotinic acid and derivatives thereof, glycyrrhizic acid and derivatives thereof (dipotassium salt, etc.) , T-cycloamino acid derivatives, vitamin A and its derivatives, vitamin E and its Derivatives, allantoin, α-hydroxy acids, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, coenzyme Q-10, α-lipoic acid, gentian extract, licorice extract, pearl barley extract, chamomile extract, carrot extract, aloe extract Herbal extract, rice extract hydrolyzate, low allergen rice extract hydrolyzate, rice bran extract hydrolyzate, rice fermented extract, green algae, red algae or brown algae seaweed extract, seaweed etc. Extract, Sahakuhi extract, Zizyphus juazeiro extract, Beech extract, Kidachi aloe extract, Mannenrou extract, Ginkgo biloba extract, Horsetail extract, Safflower extract, Panax ginseng extract, Elderberry extract, Hagoromogusa extract , Lotus extract, ma Go extract, cherimoya extract, mangosteen extract, tabebuia impetiginosa extract, citrus (citrus, yuzu, Daidai, etc.) extract, yeast extract, eggshell membrane extract protein, deoxyribonucleic acid potassium salt, sphingolipid, lotus fermentation Liquid, purple orchid root extract, murasakixikib extract, rice extract, coral grass extract, pollen extract, lily whole plant extract and the like.

  Examples of the kojic acid derivative include kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid esters such as kojic acid dibutyrate, kojic acid sugar derivatives such as kojic acid ethers, and kojic acid glucoside. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-O-α) -D-glucopyranosyl-L-ascorbine Acid) ascorbic acid sugar derivatives, acylated 6-positions of these ascorbic acid sugar derivatives (acyl groups are hexanoyl, octanoyl, decanoyl, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetra L-ascorbic acid tetrafatty acid esters such as lauric acid ester, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium and the like are hydroquinone derivatives such as arbutin (hydroquinone). -Β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like, and as resorcinol derivatives, for example, 4-n-butylresorcinol, 4-isoamylresorcinol and the like are 2,5-dihydroxybenzoic acid. Derivatives and For example, 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, etc., and nicotinic acid derivatives include, for example, nicotinic acid amide, nicotinic acid benzyl, etc. However, examples of vitamin E derivatives include vitamin E nicotinate, vitamin E linoleate, and sodium salt of vitamin E phosphate. Examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctane. There are acids.

  Examples of the anti-inflammatory agent include glycyrrhizic acid, allantoin, gentian root extract, and Tabebuia impetiginosa extract.

  Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives, ubidecaquinone (ubiquinone), rutin, rutin glucoside, white coconut extract, rice extract, murasakikibu extract, birch extract Products, hamamelis extract, oolong tea extract, black bean hydrolyzed extract, hagoromogusa extract and the like.

  Examples of the collagen synthesis promoter include ascorbic acid and derivatives thereof (such as L-ascorbic acid-2-phosphate magnesium and L-ascorbic acid-2-glucoside), rice extract hydrolyzate, carrot extract, carrot fermentation Products, eel extracts and the like.

  Next, the present invention will be described more specifically with reference to production examples, test examples, and examples (formulation examples of cosmetics), but the present invention is not limited thereto. In the following, all parts are parts by weight, and all% are% by weight.

Production Example 1 Preparation of water eggplant juice (1)
2800 g of water eggplant (from Quanzhou, the same applies hereinafter) was made into a paste with a food processor, and the resulting liquid was further filtered to obtain 1980 g of a tan clear water eggplant juice (solid concentration) 3.70%).

Production Example 2 Preparation of water eggplant juice (2)
2800 g of water eggplant fruit was made into a paste using a food processor, and the resulting liquid was decolorized with activated carbon to obtain 1700 g of a colorless and transparent water eggplant juice (solid content: 3.51%).

Production Example 3 Preparation of water eggplant fruit extract (1)
Purified water (1000 g) was mixed with 100 g of dried eggplant fruit and extracted at 40 ° C. for 4 hours, followed by filtration to obtain 820 g of a clear yellow eggplant fruit extract (solid content concentration: 1.31%).

Production Example 4 Preparation of water eggplant fruit extract (2)
100 g of dried eggplant fruit was mixed with 1200 g of a 7: 3 (weight ratio) mixture of purified water and 1,3-butylene glycol, followed by extraction at 80 ° C. for 2 hours, followed by filtration, 910 g of a fruit extract was obtained (solid content concentration 1.01%).

Production Example 5 Preparation of water eggplant fruit extract (3)
100 g of dried eggplant fruit was mixed with 800 g of a 1: 1 (weight ratio) mixture of purified water and ethanol, extracted at 4 ° C. for 72 hours, filtered, and 650 g of a light yellow clear water eggplant fruit extract was obtained. Obtained (solid content concentration 0.77%).

Production Example 6 Preparation of water eggplant fruit extract (4)
100 g of dried eggplant fruit was pulverized with a pulverizer and sieved to obtain 75 g of dried eggplant fruit powder having an average particle size of 20 μm.

Production Example 7 Preparation of water eggplant juice liquid powder 500 g of water eggplant juice liquid obtained in the same manner as in Production Example 1 was freeze-dried and then pulverized to obtain 16.8 g of tan brown water eggplant juice liquid powder.

Comparative Production Example 1 Preparation of Long Eggplant Juice Liquid In Production Example 1, 1020 g of long eggplant juice was obtained in the same manner as in Production Example 1 except that 2800 g of long eggplant was used instead of water eggplant (solid content 3.15%).

Comparative Production Example 2 Preparation of rice eggplant juice.
In Production Example 1, 980 g of rice eggplant juice was obtained in the same manner as in Production Example 1 except that 2800 g of rice eggplant was used instead of water eggplant (solid content 3.20%).

Example 1. Cream [component A] part Liquid paraffin 5.0
Hexalan (Note 1) 4.0
Paraffin 5.0
Glyceryl monostearate 2.0
Polyoxyethylene (20) sorbitan monostearate 6.0
Butylparaben 0.1
(Note 1) Technoble Co., Ltd. glyceryl trioctanoate [component B]
Juice eggplant juice from Production Example 10.0
Glycerin 5.0
Methylparaben 0.1
Moiston C (Note 2) 1.0
Amount of purified water totaling 100 parts
(Note 2) NMF component manufactured by Technoble Co., Ltd.
[C component]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After this was cooled to 50 ° C., component C was added and further stirred and mixed to obtain a cream.

Example 2 Cream A cream was obtained in the same manner as in Example 1 except that the water eggplant juice of Production Example 2 was used instead of the water eggplant juice of Production Example 1 in the component B of Example 1.

Example 3 Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Juice eggplant juice from Production Example 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Purified water Amount of 100 parts [component C]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 4 Lotion [Ingredient]
Part Juice eggplant juice of Production Example 2 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume Appropriate amount Potassium hydroxide Appropriate amount Purified water An amount that makes the total amount 100 parts The above ingredients were mixed to obtain a lotion.

Example 5 FIG. Lotion [component A] part olive oil 1.0
Polyoxyethylene (5.5) cetyl ether 0.5
Butylparaben 0.1
[B component]
Juice eggplant juice of Production Example 2 10.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Potassium hydroxide Appropriate amount Purified water Amount that makes the total amount 100 parts [Component C]
Perfume Appropriate A component and B component were each heated to 80 ° C. or higher, then B component was added to A component and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Example 6 Emulsion An emulsion was obtained in the same manner as in Example 3 except that the water eggplant juice extract of Production Example 3 was used instead of the water eggplant juice of Production Example 1 in the component B of Example 3.

Example 7 Emulsion An emulsion was obtained in the same manner as in Example 3 except that the water eggplant fruit extract of Production Example 4 was used instead of the water eggplant juice of Production Example 1 in the component B of Example 3.

Example 8 FIG. Emulsion An emulsion was obtained in the same manner as in Example 3 except that the water eggplant fruit extract of Production Example 5 was used instead of the water eggplant juice of Production Example 1 in the component B of Example 3.

Example 9 Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Juice eggplant juice from Production Example 10.0
L-ascorbic acid-2-glucoside 2.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total [Component C]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 10 Emulsion Other than using 2.0 parts of L-ascorbic acid-2-phosphate magnesium in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Example 9 Obtained an emulsion in the same manner as in Example 9.

Example 11 Emulsion In addition to using 2.0 parts of L-ascorbic acid-2-phosphate and 2.0 parts of sodium L-ascorbic acid-2-phosphate in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Example 9 Obtained an emulsion in the same manner as in Example 9.

Example 12 Emulsion In the component B of Example 9, the emulsion was prepared in the same manner as in Example 9, except that 2.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. Obtained.

Example 13 Latex In the component B of Example 9, instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide, a rice bran extract hydrolyzate (trade name “Grace Snow” manufactured by Technoble Co., Ltd.) An emulsion was obtained in the same manner as in Example 9 except that 5.0 parts of * Snow * HP ", solid content concentration 3.5%) were used.

Example 14 Latex In the component B of Example 9, in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide, white seed (Brassica Alba) seed extract (trade name, manufactured by Technoble Co., Ltd.) An emulsion was obtained in the same manner as in Example 9 except that 5.0 parts of “Sinablanca-WH” (solid content concentration 1.0%) were used.

Example 15. Emulsion Example 9 except for using 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the component B of Example 9 except that 1.0 part of γ-amino-β-hydroxybutyric acid is used. In the same manner as in No. 9, an emulsion was obtained.

Example 16 Latex In the component B of Example 9, instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide, a rice extract hydrolyzate (trade name “Oryzenoble, manufactured by Technoble Co., Ltd.) A solid emulsion was obtained in the same manner as in Example 9 except that 5.0 parts of a solid content concentration of 1.5% was used.

Example 17. Lotion [component] part water eggplant juice of production example 2 3.0
L-ascorbic acid-2-glucoside 2.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Sodium alginate 0.1
Potassium hydroxide Appropriate amount Perfume Appropriate amount Purified water An amount that makes the total amount 100 parts The above ingredients were mixed to obtain a lotion.

Example 18 Pressed powder [A component] Part Bengala 0.5
Yellow iron oxide 1.5
Black iron oxide 0.1
Titanium oxide 10.0
6-Nylon powder 4.0
Amount that makes 100 parts of sericite Mica 23.0
Talc 25.0
Mizu eggplant fruit dried product powder of Production Example 6 0.1
[B component]
Squalane 1.0
Methylpolysiloxane 4.0
Propylparaben 0.1
Dehydroacetic acid 0.1
Liquid paraffin 2.0
Fragrance Appropriate Amounts The above A component and B component were mixed and stirred, mixed, then passed through a 200 mesh Tyler mesh sieve, and the resulting mixed powder was cast into a mold to obtain a pressed powder.

Example 19. Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B component]
Water eggplant juice from Production Example 1 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [C component]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Example 20. Cream foundation [component A] part Stearic acid 5.0
Cetanol 2.0
Glyceryl monostearate 3.0
Liquid paraffin 5.0
Squalane 3.0
Isopropyl myristate 8.0
Polyoxyethylene (20) glyceryl monostearate 2.0
Propylparaben 0.1
[B component]
Mizu eggplant juice of Production Example 2 5.0
Sorbitol 3.0
1,3-butylene glycol 5.0
Triethanolamine 1.5
Methylparaben 0.1
Purified water Amount of 100 parts [component C]
Titanium oxide 8.0
Talc 2.0
Kaolin 5.0
Bentonite 1.0
Coloring pigment appropriate amount [component D]
Fragrance 0.3
Component C was mixed and pulverized with a pulverizer. The component B was mixed, and the pulverized component C was added thereto and uniformly dispersed in a colloid mill. The components A and B and C dispersed uniformly were each heated to 80 ° C., and then the components A were added to the components B and C while stirring, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, D component was added and stirred and mixed, and also it cooled to 30 degrees C or less, stirring, and obtained the cream foundation.

Example 21. Hair artic [component A] part ethanol 60.0
l-Menthol 0.5
Fragrance 0.1
Methylparaben 0.1
[B component]
Glycerin 2.0
1,3-butylene glycol 2.0
Juice eggplant fruit extract of Production Example 5 10.0
Purified water An amount that makes the total amount 100 parts The above A component and B component were each dissolved at room temperature, and then the B component was added to the A component with stirring and dissolved to obtain a hair artic.

Example 22. Hair treatment [ingredient] part stearyltrimethylammonium chloride 6.0
Polyvinylpyrrolidone 4.0
Glycerin 1.0
Ethylparaben 0.1
Water eggplant fruit extract of Production Example 3 5.0
Purified water in a total amount of 100 parts The above ingredients were heated to 80 ° C and then mixed and stirred to obtain a hair treatment.
This product was also suitable as a hair pack.

Example 23. Hair shampoo [component A] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[B component]
Citric acid 0.1
Mizu eggplant fruit extract of Production Example 4 5.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component, and stirring is continued to cool to room temperature to obtain a hair shampoo It was.

Example 24. Hair rinse [component A] Part Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[B component]
Water eggplant juice from Production Example 1 5.0
1,3-butylene glycol 5.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain hair rinse It was.

Example 25. Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B component]
Juice eggplant juice of Production Example 2 10.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Example 26. Soap [component A] part hardened castor oil 26.0
Coconut oil 10.0
Olive oil 4.0
[B component]
Sodium hydroxide 6.0
Sugar 10.0
Glycerin 5.0
Mizu eggplant juice liquid powder of Production Example 7 0.5
Purified water Amount of 100 parts [component C]
Ethanol 20.0
Perfume Appropriate A component and B component were each heated to 80 ° C. and dissolved uniformly, and then B component was added to A component to saponify. This was cooled to 50 ° C. with stirring, and component C was added. This was poured into a mold and cooled, and then dried at room temperature for several days. A sufficiently dried product was taken out from the mold to obtain soap.

Comparative Example 1 Cream A cream was obtained in the same manner as in Example 1 except that the long eggplant juice of Comparative Production Example 1 was used instead of the water eggplant juice of Production Example 1 in the component B of Example 1.

Comparative Example 2 Cream A cream was obtained in the same manner as in Example 1 except that the rice eggplant juice of Comparative Production Example 2 was used instead of the water eggplant juice of Production Example 1 in the component B of Example 1.

Test Example 1 Collagen production promotion test in epidermal cells Using water eggplant juice and water eggplant fruit extract obtained in Production Examples 1-3 and long eggplant and rice eggplant juice obtained in Comparative Production Examples 1 and 2 as samples, Each of these solutions was diluted with purified water to adjust the solid content to 1%, and the collagen production promoting action in epidermal cells was examined.

[Test method]
Human skin-derived epidermal cells PHK16-0b (Lot. 060325) were seeded at 1 × 10 ⁴ cells / hole in a 96-well microplate containing MCDB153 (manufactured by Sigma) and epidercel HKGS (manufactured by Kurabo Industries) at 37 ° C. After pre-culturing for 1 day, the sample solution was added to the medium so as to have a concentration of 1.0 or 5.0% (as a solution concentration), and further cultured at 37 ° C. for 5 days.
Next, the culture solution was removed, 0.04% fast green, 0.05% sirius red solution was added, and intracellular collagen and non-collagen were stained, and then washed thoroughly. Next, extraction was performed with methanol containing 0.1% NaOH, and the amount of collagen was measured at a wavelength of 540 nm. Moreover, the same test was done when no sample was added (control).
The results are shown as the relative values (collagen production rate) of the absorbance when each sample was added, assuming that the absorbance at a wavelength of 540 nm when no sample was added (control) was 100.

[result]
The results are shown in FIG. In FIG. 1, the symbols A to K mean the following: A: Control B: 1% addition of the juice of Production Example 1 C: Add 5% of the juice of Production 1 D: Production 1% addition zone E of the juice of Example 2: 5% addition zone of the juice of Production Example 2 F: 1% addition zone of the juice of Production Example 3 G: 5% addition zone H of the juice of Production Example 3 : 1% added section of the squeezed liquid of Comparative Production Example 1 I: added 5% of the squeezed liquid of Comparative Production Example 1 J: 1% added section of the squeezed liquid of Comparative Production Example 2 K: squeezed liquid of the Comparative Production Example 2 5% added zone

  As shown in FIG. 1, the water eggplant juice (Production Examples 1 and 2) or the water eggplant fruit extract (Production Example 3) of the present invention has an effect of significantly promoting collagen production in human skin-derived epidermal cells. Was confirmed. On the other hand, for the eggplant squeezed juice (Comparative Production Example 1) and rice eggplant squeezed juice (Comparative Production Example 2), a slight collagen production promoting effect is observed with the addition of 5%. It was not far away.

Test Example 2 Collagen production promotion test in fibroblasts For the same sample as in Test Example 1, the collagen production promotion effect in dermal fibroblasts was examined.
[Test method]
Human skin dermis-derived fibroblasts NB1RGB (Lot. 051203 (12)) were seeded at 1 × 10 ⁴ cells / hole in a 96-well microplate containing 0.5% NCS-containing minimum essential medium, and at 37 ° C. for 1 day. After pre-culture, the sample solution was added to the medium so as to have a concentration of 1.0 or 5.0% (as a solution concentration), and further cultured at 37 ° C. for 5 days.
Next, the culture solution was removed, 0.04% fast green, 0.05% sirius red solution was added, and intracellular collagen and non-collagen were stained, and then washed thoroughly. Next, extraction was performed with methanol containing 0.1% NaOH, and the amount of collagen was measured at a wavelength of 540 nm. In addition, L-ascorbic acid-2-phosphate magnesium (APM) was used instead of the sample solution as a test for confirming whether the test system was functioning normally when the sample was not added (control). The same test was performed for each case where 2 mM was added.
The results are shown as the relative values (collagen production rate) of the absorbance when each sample was added, assuming that the absorbance at a wavelength of 540 nm when no sample was added (control) was 100.

[result]
The results are shown in FIG. In FIG. 2, the symbols L to V mean the following: L: Control M: 1% addition zone of the juice of Production Example 1 N: 5% addition zone of the juice of Production Example 1 O: Production 1% addition zone P of the juice of Example 2 5% addition zone of the juice of Production Example 2 Q: 1% addition zone of the juice of Production Example 3 R: 5% addition zone S of the juice of Production Example 3 : 1% added section of the juice of Comparative Production Example 1 T: 5% added section of the juice of Comparative Production Example 1 U: 1% added section of the juice of Comparative Production Example 2 V: Juice of the Comparative Production Example 2 5% added zone

  As shown in FIG. 2, the water eggplant juice (Production Examples 1 and 2) and the water eggplant fruit extract (Production Example 3) of the present invention have the effect of significantly promoting collagen production in normal human-derived fibroblasts. Was confirmed. On the other hand, the collagen production promoting action exhibited by the squeezed eggplant juice (Comparative Production Example 1) and the rice eggplant juice (Comparative Production Example 2) was far from the sample of the present invention.

Test Example 3 Skin irritation [sample]
(1) Kneaded eggplant juice from Production Example 1 kneaded with JP Vaseous Petroleum to a concentration of 5% (Invention Sample 1)
(2) Kneaded eggplant juice from Production Example 2 kneaded with JP Petroleum Vaseline to a concentration of 5% (Invention Sample 2)
(3) Water eggplant fruit extract of Production Example 3 kneaded to JP Hydrophilic Vaseline to a concentration of 5% (Invention Sample 3)
(4) Kneaded eggplant fruit extract of Production Example 4 kneaded in JP Hydrophilic Petrolatum to a concentration of 5% (Invention Sample 4)
(5) JP Hydrophilic Petrolatum (control)

[Test method]
Five adult males aged 20 to 50 years were used as subjects, and the inner side of each upper arm was wiped with ethanol to remove sebum, and the samples 1 to 4 of the present invention and the control bureau were placed on the aluminum plate of the fin chamber. What applied each 0.2g of hydrophilic petrolatum was stuck. After 24 hours, the fin chamber was removed, and the degree of skin irritation was determined by the method and criteria described below.
[Judgment]
After 1 hour, 24 hours and 48 hours after removing the patch, the state of erythema and edema at the applied site was visually determined based on the following “skin irritation criteria by dreze method”, and the average value of 5 subjects Asked.
(Erythema)
Score Skin condition 0: No erythema 1: Extremely mild erythema 2: Clear erythema 3: Moderate to strong erythema 4: Light crust formation in strong crimson erythema (edema)
Score Skin condition 0: No edema 1: Extremely mild edema 2: Clear edema (clearly distinguishable from surroundings)
3: Moderate edema (swelling of 1 mm or more)
4: Strong edema (further spread around)

The results are shown in Table 1.

  As is apparent from the results in Table 1, the water eggplant juice and the water eggplant fruit extract of the present invention have no skin irritation and are extremely safe.

Test Example 1 Monitor Test The effects of the creams of Examples 1 and 2 and Comparative Example 1 and 2 on the skin were examined by a monitor test.
[Test method]
Randomly extracted 40 women aged 18-50 were divided into two groups (A, B) of 20 subjects as subjects, and each group was divided into Example 1 and Comparative Example 1 cream or Example 2. One of the two combinations of the cream of Comparative Example 2 was allocated, and the cream of the Example or Comparative Example was applied to the left and right cheeks twice a day (morning and evening) for one month. Then, the improvement effect with respect to fine wrinkles and the improvement effect with respect to skin tension and gloss were evaluated based on the following evaluation criteria.

[Evaluation criteria]
(Improvement effect on small wrinkles)
A: Almost inconspicuous B: Almost inconspicuous C: Slightly inconspicuous D: Unchanged E: On the contrary (Improvement effect on skin elasticity and gloss)
A: Clearly improved B: Much improved C: Slightly improved D: No change E: On the contrary

[result]
The results are shown in Table 2. In addition, the number of each evaluation column of AE in a table | surface shows the number of the test subjects who performed the said evaluation in 20 test subjects.

As shown in Table 2, the cream of the present invention, which is prepared by blending squeezed water eggplant juice as an active ingredient, is excellent in improving wrinkles and sagging, which are typical symptoms of skin aging or unhealing. Indicates.
On the other hand, in the cream of the comparative example which mix | blended the juice of long eggplant or rice eggplant, the improvement effect was hardly seen but it resulted in a difference in effectiveness clearly.

These are graphs showing the collagen production rate of epidermal cells in each test section of Test Example 1. (Vertical axis: Collagen production rate (%)) These are the graphs which show the collagen production rate of the dermal fibroblast in each test section of Test Example 2. (Vertical axis: Vertical axis: Collagen production rate (%))

Explanation of symbols

A: Control B in Test Example 1 B: 1% addition of squeezed liquid of Production Example 1 in Test Example 1 C: 5% addition of squeezed liquid of Production Example 1 in Test Example 1 D: Test 1% of the squeezed liquid of Production Example 2 in Example 1 E: 5% of the squeezed liquid of Production Example 2 in Test Example 1 F: Production of Test Example 1 in Test Example 1 1% addition section G of the juice of Example 3: 5% addition section of the juice of Production Example 3 in Test Example 1 H: 1% addition section I of the juice of Comparative Production Example 1 in Test Example 1 : 5% addition zone of juice of Comparative Production Example 1 in Test Example 1 J: 1% addition zone of juice of Comparative Production Example 2 in Test Example K: Comparative Production Example in Test Example 1 5% added section of the squeezed liquid of No. 2: control M in Test Example 2 M: added 1% of the squeezed liquid of Production Example 1 in Test Example 2 N: squeezed of Production Example 1 in Test Example 2 5% of juice Ka: O: 1% of the juice of the squeezed liquid of Production Example 2 in Test Example 2: P: 5% of the squeezed liquid of Production Example 2 of Test Example 2: Q: Test Example 2: 1% of the squeezed liquid of Production Example 3 in Production Example R: 5% of the squeezed liquid of Production Example 3 in Experimental Example 2 S: The squeezed liquid of Comparative Production Example 1 in Experimental Example 2 1% addition zone T: 5% addition zone of squeezed liquid of Comparative Production Example 1 in Test Example 2 U: 1% addition zone of squeezed liquid of Comparative Production Example 2 in Test Example 2 V: In Test Example 2 5% of the juice of Comparative Production Example 2

Claims (3)

An anti-aging cosmetic comprising one or more selected from squeezed liquid and / or an extract of water eggplant (Solanum Melongena; Egg plant cv 'Mizu-nasu'). The anti-aging cosmetic composition according to claim 1, wherein the squeezed juice and extract of mizu eggplant have a collagen production promoting action of epidermal cells and a collagen production promoting action of dermal fibroblasts. Collagen production promoter of epidermal cells and dermal fibroblasts containing one or more selected from squeezed squeezed liquid and / or extract as active ingredients.