JP4734093B2 - Cosmetics - Google Patents

Description

  The present invention relates to a cosmetic effective for preventing skin aging and preventing or improving rough skin. More specifically, the present invention has both a dermal fibroblast activation action and a collagen synthesis promoting action, and the synergistic effect of these actions prevents skin aging. It has a high effectiveness in preventing and improving rough skin and contains natural ingredients with excellent biological safety, and is useful for maintaining or improving the skin in a healthy and youthful state. Related to cosmetics.

Skin aging is caused by internal factors such as inactivation of cell proliferation / differentiation with age, decreased hormone secretion, and damage of cells / tissues caused by active oxygen induced by sunlight (ultraviolet rays) or extracellular matrix components. This is a phenomenon that is caused by complex intertwining of external factors such as quantitative decrease in inflammation and inflammation. Of these, aging of the skin based on internal factors causes skin tissue flexibility due to decreased activity of dermal fibroblasts and decreased proliferation ability, as well as decreased extracellular matrix components such as collagen, elastin, and hyaluronic acid. Loss of elasticity and inactivation of the epidermal basal cells, resulting in a decrease in the skin's stratum corneum barrier function, sebum secretion function, and water retention function. Skin morphological and physiological changes include wrinkles and tarmi. Appears as symptoms such as rough skin.
In order to prevent this skin aging and keep the skin healthy and youthful, the use of various active ingredients has been proposed and cosmetics containing these ingredients have been put on the market. For example, antioxidants such as vitamin C and vitamin E, anti-inflammatory agents such as glycyrrhizic acid, various ultraviolet absorbers, α-hydroxycarboxylic acid, placental extract, and cell activation such as γ-amino-β-hydroxybutyric acid Ingredients, extracellular matrix components such as collagen, elastin and hyaluronic acid, humectants such as urea, and the like.
However, since these ingredients generally only serve to prevent or improve one of the above-mentioned skin aging factors, depending on the conventional cosmetics containing these ingredients, the skin aging that is truly satisfactory At present, the prevention effect cannot be obtained. In addition, depending on the type of component, there are problems such as having skin irritation and skin sensitization, and there are problems with safety against living bodies, or cell activation and proliferation are not sufficient.

  In view of the above-mentioned problems seen in conventional cosmetics, the present inventors have combined and multifaceted action against various factors such as skin aging and rough skin, thereby preventing skin aging and rough skin. As a result of diligent research to find new cosmetic ingredients that are highly effective for prevention and improvement, and that are also sufficiently safe for the skin, germinated brown rice koji extract and its enzymatic degradation The product or fermented product has both the dermal fibroblast activation effect and the collagen synthesis promotion effect in the dermis, and is also an ingredient derived from rice, so it has excellent biological safety and meets the above objectives. As a result, the present invention has been completed.

Various proposals have been made for using rice bran or rice bran-derived ingredients as cosmetic ingredients.
For example, Japanese Patent Application Laid-Open No. Sho 53-015434 discloses a skin cosmetic that has an effect of promoting metabolism of skin, including a culture obtained by inoculating and culturing koji mold on rice bran. The gazette includes a skin beautifying agent obtained by roasting rice bran and extracted with alcohol, and Japanese Patent Application Laid-Open No. 62-187404 contains a sphingoglycolipid obtained from rice bran and a steroid glycoside or triterpenoid glycoside. An effective cosmetic material is disclosed in JP-A-62-106008 and JP-A-01-093521 in which a bath agent from rice bran is incorporated, and in JP-A-01-066106, a polysaccharide contained in rice bran is incorporated. Japanese Patent Laid-Open No. 02-053705 discloses a cosmetic material effective for improving rough skin, which contains a water-soluble antioxidant obtained from rice bran raw material, and is used to prevent and improve skin aging and skin function damage due to oxidation. An effective cosmetic is disclosed in JP 04-368314 A which contains rice bran or an extract thereof. In addition, a patch exhibiting a skin improving action and a skin beautifying action is disclosed in Japanese Patent Laid-Open No. 05-221844, an anti-aging cosmetic containing an enzymatic degradation product of an alkaline extract of rice bran is disclosed in Japanese Patent Laid-Open No. 07-304648 and Japanese Laid-Open Patent Publication No. 2000-264834 discloses a whitening cosmetic containing a neutral rice medium extract and an acidic aqueous medium extract as active ingredients, and Japanese Laid-Open Patent Publication No. 2004-083594 discloses a neutral rice bran medium. Whitening cosmetics containing enzyme-decomposed degradation products of the extract are disclosed.
However, none of these known documents specifically disclose the use of rice bran obtained from germinated brown rice according to the present invention as rice bran.
Moreover, as shown in Test Examples 1 and 2 later, the fibroblast activation action and collagen synthesis promoting action of the koji extract are remarkably recognized in the koji of the germinated brown rice, especially in the rice koji. Of course, there is no description in the known literature, nor is there any suggestion of this.

On the other hand, several proposals have also been made regarding the use of germinated rice as a raw material for cosmetics, such as JP-A Nos. 07-002628, 07-10733, 07-41426, 07-187992, and 07. -252129 and 08-119870 disclose germinated rice extract, germinated rice or enzyme or koji-treated product of the extract, fermented product thereof, hair restorer, moisturizer, It is disclosed that they are used as active ingredients of anti-aging cosmetics, active oxygen scavengers, bathing agents, epidermal stratum corneum cleansing agents and turnover normalizing agents, respectively.
However, the cosmetic effect exhibited by these known inventions is not limited to bathing agents, epidermal stratum corneum cleansing agents, or turnover normalizing agents, but also to moisturizing and anti-aging cosmetics described in JP 07-010733 A. As it is clear from the fact that only the skin groove and skin mound health effects are mentioned, both are related to the health and health of the epidermis, or JP 07-41426 A Like active oxygen scavengers, it is related to the improvement of skin aging and rough skin based on external factors such as active oxygen induced by ultraviolet rays, and it activates dermal fibroblasts, which is indicated by the components derived from rice bran The action prevents aging of the dermis due to internal factors such as aging, cell and tissue activity decline, and further promotes the synthesis of collagen to maintain skin elasticity and reduce skin roughness and gloss. It sounds like preventing the occurrence of wrinkles, the skin from the inside to the cosmetic and the of the present invention to healthy ones, are different from the effect obtained clearly action mechanism and thereby.
In addition, in these known inventions, the effectiveness of the above-mentioned stratum corneum sounding action has been confirmed only for the endosperm portion of germinated rice, and what specifically is the effectiveness of the buttocks? Therefore, from these known inventions, the technical idea that the germinated brown rice bran is actively used as a cosmetic ingredient cannot be read at all.

  According to the current state of the above prior art, the germinated brown rice bran extract used as an active ingredient in the present invention and its enzymatic degradation product or fermented product have a remarkable dermal fibroblast activation action and collagen synthesis promoting action, It is clear that it cannot be predicted at all about the excellent effect of preventing skin aging, preventing rough skin, and improving the skin by making the dermis healthy and healthy by the synergistic effect of these two actions.

JP-A-53-015434 JP 59-154971 A JP 62-187404 A JP 62-106008 A Japanese Unexamined Patent Publication No. 01-093521 Japanese Unexamined Patent Publication No. 01-066106 Japanese Patent Laid-Open No. 02-053705 JP 04-368314 A Japanese Patent Laid-Open No. 05-221844 Japanese Unexamined Patent Publication No. 07-304648 JP 2000-264834 A JP 2004-083594 A JP 07-002628 A JP 07-010733 A JP 07-041426 A Japanese Unexamined Patent Publication No. 07-187992 JP 07-252129 A Japanese Unexamined Patent Publication No. 08-119870

That is, the present invention is bran of brown rice, a cosmetic which is characterized in that the extract was formulate one or more kinds selected from enzymatic degradation thereof bran or extract and fermentation thereof bran or extract Is .
Here, the germinated brown rice refers to a rice mainly composed of germ and endosperm obtained when the germinated brown rice is made into germinated rice, but it may contain some components of endosperm. Absent.
In the present invention, the term cosmetic is used in a broad sense including so-called cosmetics and quasi-drugs.

The germinated brown rice bran or extract thereof has both the dermal fibroblast activation effect and the collagen production promoting effect. By the synergistic anti-aging effect based on the combination of these effects, the cosmetic of the present invention Improves the elasticity and firmness of the skin, prevents or ameliorates skin aging and unhealthy symptoms such as wrinkles and tarmi, and maintains the skin in a truly healthy and youthful state. In addition, the enzyme decomposition product or fermented product of the above koji or its extract not only has the strong dermal fibroblast activation action and collagen synthesis promoting action similar to koji or the extract itself, but also an enzyme treatment or fermentation treatment. As a result, the characteristics of the extract, for example, the flow characteristics of an extract solution are improved. The solid-liquid separation operation such as the above becomes easy, and there is an advantage that the working efficiency in the manufacturing process is greatly improved.
Further, the above-mentioned active ingredient used in the cosmetic of the present invention has no irritation to the skin, and therefore the cosmetic of the present invention is very excellent in biological safety.

Hereinafter, the present invention will be described in detail.
In the present invention, germinated brown rice, such as Koshihikari, Sasanishiki, Akitakomachi, human mebore, etc. The resulting soot can be used.
Although there is no particular limitation on the range of the milling ratio in germination brown rice, the milling ratio is generally 80% or more, particularly 90% or more, from the viewpoint of the effectiveness of the rice bran or the extract. It is preferable to use rice bran obtained as such a rice milling ratio in the present invention.

The cosmetics of the present invention include the above germinated brown rice koji itself or an extract obtained by extracting this with an extraction solvent, and further, one or more of these koji or an enzymatic degradation product or fermented product of the extract. Is blended as an active ingredient.
As described above, the enzyme-decomposed product or fermented product not only has excellent fibroblast activation and collagen synthesis promoting effects similar to those of koji or extract, but also has been subjected to enzyme treatment or fermentation treatment. In addition, the odor peculiar to rice bran is reduced, or the flow characteristics of the extract are improved, and the production efficiency is improved.

  The extract of germinated brown rice bran is obtained by subjecting germinated brown rice bran to an extraction solvent by an appropriate method such as a dipping method or a countercurrent extraction method after subjecting it beforehand to a pulverization treatment and a degreasing treatment. It can be prepared by contacting. A supercritical extraction method may also be used.

As an extraction solvent, water; lower alcohols such as methanol, ethanol, and propanol; higher alcohols such as oleyl alcohol, stearyl alcohol, and octyldodecanol; many solvents such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin Monohydric alcohols; esters such as ethyl acetate, butyl acetate, methyl propionate and glyceryl trioctanoate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; carbonization such as n-hexane, toluene and chloroform Examples thereof include hydrogen-based solvents, which are used alone or in combination of two or more.
In addition, from the viewpoint of the fibroblast activation action or collagen production promotion action of the obtained extract, in the present invention, among these solvents, those having a particularly high polarity are preferably used. Furthermore, it is preferable to use one kind of single solvent or two or more kinds of mixed solvents selected from water, lower alcohols and polyhydric alcohols from the viewpoint that they can be widely applied to cosmetics. Is most preferably used alone.

  The mixing ratio in the case of using a mixed solvent is, for example, 1: 1 to 25: 1 by volume ratio (hereinafter the same) if the mixed solvent is water and ethyl alcohol, and 1: if the mixed solvent is water and glycerin. In the case of a mixed solvent of 1 to 20: 1, or water and 1,3-butylene glycol, it is preferably in the range of 1: 1 to 20: 1.

  In the preparation of the extract of the present invention, the pH of the extract is preferably maintained in the range of 5 to 9. In this sense, if necessary, the above extraction solvent may contain sodium hydroxide, sodium carbonate, potassium hydroxide. Alternatively, an alkaline adjusting agent such as arginine, an acidic adjusting agent such as citric acid, hydrochloric acid, phosphoric acid, sulfuric acid, or the like may be blended to adjust to a desired pH.

  Extraction conditions such as extraction temperature and time vary depending on the type of solvent used, the extraction method, and the like. For example, in the case of the immersion method, the extraction temperature is generally 1 to 100 ° C., preferably 30 to 80 ° C. In addition, the extraction time is preferably about 0.1 to 24 hours, particularly about 0.5 to 3 hours.

  When the above extraction process is completed, the liquid phase is separated by applying solid-liquid separation means such as filtration and centrifugation, and the pH is generally adjusted to 4 to 8 which is a preferable range as a cosmetic ingredient. In addition, it is blended in cosmetics as it is or after adjusting to an appropriate concentration by dilution or vacuum concentration. If necessary, this liquid phase may be pulverized according to a conventional method such as a spray drying method or a freeze drying method and then blended into a cosmetic. Further, depending on the case, an extraction stock solution before applying the above-described solid-liquid separation means can be used.

  In the case of preparing an enzymatic degradation product by subjecting germinated brown rice bran or its extract to enzymatic degradation, enzymes include proteolytic enzymes such as actinase and papain, starch degrading enzymes such as glucoamylase and α-amylase, cellulase, hemi One or more selected from fibrinolytic enzymes such as cellulase and pectinase and lipolytic enzymes such as lipase are used, and in particular one or more enzymes selected from these four enzyme groups, respectively. Are preferably used in combination.

Enzymatic degradation treatment of germinated brown rice bran or its extract with those enzymes, if the bran itself or extract powder is used as the object to be treated, such as water or a mixture of water and lower alcohol or polyhydric alcohol, etc. Medium, preferably suspended or dissolved in water, and if an extract solution is used, it is used as it is, or after adjusting the concentration if necessary, an enzyme is added thereto, It is carried out by keeping the temperature around the optimum temperature of the enzyme for 1 to 24 hours.
In this case, if the extraction solvent used to prepare the extract solution may interfere with the enzyme reaction, the solvent is once distilled off, and the extract powder obtained here is preferably redissolved or dispersed in water. Enzyme treatment.
The amount of the enzyme added is in the range of 0.01 to 10% by weight in total, preferably 0.1 to 5% by weight, based on the solid content, when cocoon itself is used as an object to be treated. Moreover, when using the extract of persimmon, it is similarly 0.001-5 weight% in total with respect to solid content, Preferably it is the range of 0.01-2 weight%.

  When the above enzymatic decomposition treatment is completed, the treatment liquid is subjected to a heat treatment to deactivate the enzyme, and then the liquid obtained here is collected as it is or by filtration, centrifugation, etc. If necessary, after pH adjustment, concentration adjustment, or powdering treatment, etc., it is used as a raw material for cosmetics.

On the other hand, the fermentation treatment of germinated brown rice bran or its extract is performed, for example, as follows.
That is, firstly, a fermented solution is prepared by dissolving or suspending the fermented material in the medium. In this case, if the persimmon itself or the extract powder is used as the fermented product, as in the case of the enzymatic decomposition treatment, They are suspended or dissolved in a medium such as water, a water / lower alcohol mixture or a water / polyhydric alcohol mixture, preferably water, to obtain a fermentation treatment solution. On the other hand, if the extract solution is to be fermented, it may be used as it is, or if necessary, by adjusting the concentration, replacing the solvent, etc. to obtain a fermentation treatment solution.

Next, this fermentation treatment liquid is subjected to a sterilization treatment prior to fermentation to remove various germs that hinder fermentation.
As a method for removing this germ, it is common to use a heat sterilization method in which the fermentation treatment liquid is heat-treated, but instead, a method of preparing the fermentation treatment liquid itself as originally sterile, for example, germination If the brown rice bran itself is to be fermented, a method may be used in which the bran is sterilized in advance with sterilized ethanol and then suspended in a sterile medium such as sterile water.
As the heat sterilization treatment, the autoclave sterilization method in which the fermentation treatment solution is heated at 120 to 130 ° C. for 10 to 20 minutes, or the intermittent sterilization of holding at 80 to 90 ° C. for 60 to 120 minutes is repeated once a day for 2 to 3 days. A method such as the method is generally used.

Next, this fermentation treatment liquid is put into a fermentation tank, inoculated with bacteria and fermented. As the fungus, for example, one or two or more fungi selected from lactic acid bacteria, koji molds, yeasts, natto fungi, tempeh fungi and the like are used. The amount of inoculation is generally in the range of 10 ⁷ to 10 ⁸ / mL with respect to the fermentation treatment solution.

  Examples of lactic acid bacteria used for fermentation include Lactobacillus plantarum, L. brevis, Lactobacillus Lactobacillus lactic acid bacteria such as Lactobacillus casei; Carnobacterium divergens ), Lactic acid bacteria of the genus Carnobacterium such as C. piscicola; Leuconostoc genus such as Leuconostoc mesenteroides and L. citreum Lactic acid bacteria; Streptococcus faecalis, Streptococcus pyogenes, and other Streptococcus lactic acid bacteria; Enterococcus caseliflavus, Enterococcus caseliflavus, Ento Lactococcus genus Enterococcus such as E. sulfreus; Lactococcus plantarum Lactococcus lactic acid bacteria such as Lactococcus plantarum Lactococcus lactic acid bacteria Weissella confusa, lactic acid bacteria of the genus Weissella (W. kandleri), etc .; lactic acid bacteria of the genus Atopobium (Atopobium minutum), Atopobium (A. parvulus), etc .; Vagocovia cc ), Lactic acid bacteria of the genus Vagococcus (V. salmoninarum); Pediococcus damnosus, pediococcus Pedosaceus (P. pentosaceus), etc. Among these lactic acid bacteria, the use of Lactobacillus plantarum is preferable from the viewpoint of the action effect of the obtained fermented product and the ease of handling. .

  As the koji mold used for fermentation, for example, Aspergillus oryzae, Aspergillus flavus, Aspergillus polyoxogenes, Aspergillus sojae, Aspergillus sojae, Aspergillus sperm awamori), Aspergillus kawauchii, Aspergillus usami, Aspergillus niger and other black koji molds, Monascus anka, Monascus pilosus, etc. Any Aspergillus oryzae can be used, but among them, Aspergillus oryzae is most preferable because the color of the fermentation broth is light and the fermentation odor is relatively small.

Examples of yeast used for fermentation include Saccharomyces cerevisiae (Saccharomy
ces cerevisiae), Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, Saccharomyces baorus, Saccharomyces baorus, etc. delbruekii), Torulaspora fermentati, Torulaspora rosei, and other yeasts of the genus Torlaspora; Zygosaccharomyces rouxii; , Yeasts belonging to the genus Digosaccharomyces such as Zygosaccharomyces miso and Zygosaccharomyces lactis Any yeast, including Candida versatilis, Candida etchellsii, Candida kefyr, Candida sake, Candida scottii, and other Candida scottii yeasts However, Saccharomyces cerevisiae is most preferable because it is most widely used for foods and has a strong fermenting power.

  As Bacillus natto used for fermentation, for example, bacteria of the genus Bacillus such as Bacillus natto, Bacillus subtilis, Bacillus circulans, etc. can be used. Bacillus natto is most preferable because it is used and has high safety.

  Examples of tempeh bacteria used for fermentation include Rhizopus azygosporus, Rhizopus microsporus chinensis, Rhizopus microsporus oligosporus, Rhizopus microsporus oligosporus, Rhizopus microsporus oligosporus, (Rhizopus oryzae) and other fungi of the genus Rhizopus can be used. Among them, Rhizopus microsporus is widely used in fermented foods in Indonesia and other Southeast Asian regions. oligosporus) and Rhizopus oryzae are most preferred.

  The fermentation temperature is generally in the range of 5 to 50 ° C., preferably in the range of 35 to 40 ° C., which is the optimal temperature for lactic acid bacteria, koji molds, natto and tempe bacteria, and in the case of yeast, It is the range of 25-35 degreeC which is suitable temperature. The fermentation period is generally in the range of 3 hours to 10 days, preferably 10 hours to 3 days, at any optimum temperature.

In addition, in performing the above fermentation treatment, pre-enzyme is added before or simultaneously with the inoculation of each fungus so that the germinated brown rice bran component can be used more effectively as an assimilation source of each fungus. In addition, it is preferable to subject the germinated brown rice bran or its extract to an enzymatic hydrolysis treatment, and in particular, when the germinated brown rice bran itself is to be fermented, it is effective to use such enzyme treatment in combination. .
The enzyme species and treatment conditions used for this treatment are the same as in the case of the above-described enzymatic decomposition treatment, and there is no problem.

  If the above fermentation treatment is completed, if the sterilization of each bacterium and the enzyme treatment are used in combination, the enzyme is deactivated and the fermentation broth is subjected to a heat sterilization treatment at 70 to 100 ° C. for about 10 to 120 minutes. After application, the liquid phase is separated as it is, or generally and preferably by solid-liquid separation means such as filtration or centrifugation, and if necessary, the pH is adjusted to pH 6-8, which is the pH range of ordinary cosmetics. It is adjusted and, if necessary, adjusted to an appropriate concentration by dilution or concentration, and then used as a cosmetic raw material. In some cases, the liquid phase after solid-liquid separation may be blended into a cosmetic after being powdered according to a conventional method such as spray drying or freeze drying.

  Examples of cosmetics comprising the germinated brown rice bran of the present invention, its extract, or their enzymatic degradation product or fermented product include basic cosmetics such as emulsions, creams, lotions, essences, packs, facial cleansers, lipsticks, Makeup cosmetics such as foundations, liquid foundations, makeup press powders, hair shampoos, hair rinses, hair treatments, conditioners, hair dyes, hair conditioners, and other hair cosmetics, facial cleansers, body shampoos, soaps, etc. And bath additives. It can also be in the form of a patch.

  The blended amount of germinated brown rice bran, its extract or enzymatic degradation product or fermented product thereof in the cosmetic composition of the present invention is generally 0.001 to 5% by weight, for example, for basic cosmetics, Preferably in the range of 0.01 to 3% by weight, for makeup cosmetics generally in the range of 0.001 to 5% by weight, preferably in the range of 0.01 to 2% by weight, for hair cosmetics in general 0.001 to In the range of 10% by weight, preferably 0.01-5% by weight, and for cosmetics for cleaning, it is generally in the range of 0.001-10% by weight, preferably 0.01-5% by weight.

In addition to the above essential components, the cosmetics of the present invention include components usually used in cosmetics, such as oily components, surfactants, moisturizers, thickeners, antiseptic / disinfectants, powder components, ultraviolet absorption. Agents, antioxidants, pigments, fragrances and the like can be appropriately blended as necessary.
Moreover, as long as the effectiveness and the features of the essential components of the present invention are not impaired, other physiologically active components may be blended together.

  Here, as the oil component, for example, olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadow foam oil, sheer butter, tea tree oil, avocado oil, Oils derived from plants such as macadamia nut oil and plant-derived squalane; Fats derived from animals such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, lanolin; liquid paraffin, petrolatum, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, palmitic acid Isopropyl, me Butyl phosphate, 2-ethylhexyl glycerides, higher fatty acid octyldodecyl (octyl stearate dodecyl and the like), and the synthetic esters and synthetic triglycerides such like.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, polyoxyethylene Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty acid amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, Polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates, etc. ON surfactant: quaternary ammonium salt, primary to tertiary fatty acid amine salt, trialkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-, N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine Amphoteric surfactants such as alkylene ammoniocarboxybetaine and N-acylamidopropyl-N ′, N′-dimethyl-N′-β-hydroxypropylammoniosulfobetaine can be used.
In addition, as emulsifiers or emulsifiers, stevia derivatives such as enzyme-treated stevia, lecithin and derivatives thereof, lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented cereals (wheat, beans, millet, etc.), juiziro (Rhamnaceae ) An extract or the like can also be blended.

  Examples of the humectant include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidonecarboxylate, N- (3,4,5,6-tetrahydroxy-2- Oxohexyl) pyroglutamic acid, etc., sugars such as trehalose, lactic acid bacteria fermented rice, mucopolysaccharides (for example, hyaluronic acid and its derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and Its derivatives, hydrolyzed silk protein, NMF related substances, lactic acid, urea, higher fatty acid octyldodecyl, phytosterol, soybean phospholipid, cholesteryl isostearate, seaweed extract, seafood-derived collagen and Derivatives, various amino acids and their derivatives (such as trimethyl glycine, etc.), Byakkyuu extract, soybean milk fermented liquor, natto extract, rice-derived extract and fermented, and the like.

  Examples of thickeners include, for example, brown algae such as alginic acid, agar, carrageenan, fucoidan, green algae or red algae-derived components, beech extract, pectin, locust bean gum, polysaccharides such as aloe polysaccharide, xanthan gum, tragacanth gum, guar gum, etc. Synthetic polymers such as gums, cellulose derivatives such as carboxymethylcellulose, hydroxyethylcellulose, polyvinyl alcohol, polyvinylpyrrolidone, carboxyvinyl polymer, acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives, polyglutamic acid and its derivatives, Examples thereof include sugar compounds mainly composed of glucosyl trehalose and hydrolyzed hydrogenated starch.

  Examples of the antiseptic / bactericidal agent include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzaza chloride Examples thereof include Luconium, Salicylic acid, Ethanol, Undecylenic acid, Phenols, Jamal (Imidazodenyl urea), 1,2-pentanediol, various essential oils, bark dry product, propolis extract, methylisothiazolinone.

  Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, 6- or 12-nylon powder, polyethylene powder. , Silk powder, cellulosic powder, grains (rice, wheat, corn, millet, etc.) powder, beans (soybean, red beans, etc.) powder, etc.

  Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4 -Methoxybenzophenone-5-sulfonate, 4-tertiarybutyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. .

  Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and its derivatives, ubidecaquinone (ubiquinone), rutin, rutin glucoside, white coconut extract, rice extract, murasakikibu extract, birch extract Products, hamamelis extract, oolong tea extract, black bean hydrolyzed extract, hagoromogusa extract and the like.

  Examples of physiologically active ingredients include whitening ingredients such as t-cycloamino acid derivatives, kojic acid and derivatives thereof, ascorbic acid and derivatives thereof, hydroquinone derivatives, ellagic acid and derivatives thereof, resorcinol derivatives, sucha akuhi extract, yukinoshita extract, rice bran Extract, rice bran extract hydrolyzate, lactic acid bacteria fermented rice, lactic acid bacteria fermented germinated rice, lactic acid bacteria fermented cereals (wheat, legumes, minor grains), white coconut hydrolyzed extract, murasakixikibu extract, lotus seed fermented product, party participation Extract, Pandanus amaryllifolius Roxb extract, Arcangelicia flava Merrilli extract, chamomile extract (trade name: Chamomile ET), ginger extract Japanese knotweed extract, Sahby foliage extract, licorice extract, dandelion extract, artea extract, genus shochu extract, yukinoshita extract, jujube extract, peonies extract, suzuki extract, peach extract, citrus (e.g. mandarin orange, citrus) , Daidai, pomelo, etc.) and its isolated substances (eg, nariltin, hesperidin, etc.), green algae, red algae or brown algae seaweed extracts, seaweed extracts such as sea eels, linoleic acid and its derivatives or processing Products (for example, liposomal linoleic acid), 2,5-dihydroxybenzoic acid derivatives, etc., and skin aging preventing / beautifying components such as collagen derived from animals or fish and derivatives thereof, elastin and derivatives thereof, nicotinic acid and derivatives thereof Derivatives, glycyrrhizic acid and its derivatives (dipotassium salts, etc.), t-cycloa Mino acid derivatives, vitamin A and derivatives thereof, vitamin E and derivatives thereof, allantoin, α-hydroxy acids, diisopropylamine dichloroacetate, γ-amino-β-hydroxybutyric acid, coenzyme Q-10, α-lipoic acid, gentian extract, Herbal extracts such as licorice extract, pearl barley extract, chamomile extract, carrot extract, aloe extract, rice extract hydrolyzate, low allergen rice extract hydrolysate, rice bran extract hydrolyzate, rice fermented extract, green algae, red Seaweed extract of algae or brown algae, seagrass extract such as sea cucumber, Sakuhakuhi extract, Zizyhus juazeiro extract, beech extract, Kidachi aloe extract, mannenrou extract, ginkgo extract, horsetail extract, safflower Extract, ginseng extract, sei Elderberry extract, Chrysalis extract, Astragalus extract, Mango extract, Cherimoya extract, Mangosteen extract, Tabebuia impetiginosa extract, citrus (eg, mandarin orange, yuzu, Daidai, pomelo etc.) extract and its isolated substance (E.g., nariltin, hesperidin, etc.), yeast extract, eggshell membrane extract protein, potassium deoxyribonucleic acid, lotus fermentation broth, purple orchid root extract, murasakikibu extract, rice extract, coral grass extract, pollen extract, lily whole There are herbal extracts.

  Examples of the kojic acid derivative include kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid esters such as kojic acid dibutyrate, kojic acid sugar derivatives such as kojic acid ethers, and kojic acid glucoside. However, as the ascorbic acid derivatives, for example, L-ascorbic acid-2-phosphate sodium, L-ascorbic acid-2-phosphate magnesium, L-ascorbic acid-2-sulfate sodium, L-ascorbic acid-2 -Ascorbic acid ester salts such as magnesium sulfate, L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-O-α) -D-glucopyranosyl-L-ascorbine Acid) ascorbic acid sugar derivatives, acylated 6-positions of these ascorbic acid sugar derivatives (acyl groups are hexanoyl, octanoyl, decanoyl, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetra L-ascorbic acid tetrafatty acid esters such as lauric acid ester, 3-O-ethylascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium and the like are hydroquinone derivatives such as arbutin (hydroquinone). -Β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside) and the like, and as resorcinol derivatives, for example, 4-n-butylresorcinol, 4-isoamylresorcinol and the like are 2,5-dihydroxybenzoic acid. Derivatives and For example, 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, etc., and nicotinic acid derivatives include, for example, nicotinic acid amide, nicotinic acid benzyl, etc. However, examples of vitamin E derivatives include vitamin E nicotinate, vitamin E linoleate, and sodium salt of vitamin E phosphate. Examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctane. There are acids.

  Next, although a manufacture example, a test example, and an Example (formulation example of cosmetics) are given and this invention is demonstrated further more concretely, this invention is not limited to them. In the following, all parts are parts by weight, and all% are% by weight.

Production Example 1 Preparation of germinated brown rice bran extract 100 g of germinated brown rice bran was mixed with 1000 g of purified water, extracted at 50 ° C. for 3 hours, and then filtered to obtain 323 g of a light brown transparent germinated brown rice bran extract solution ( Solid content concentration 1.52%).

Production Example 2 Preparation of Sprouted Brown Rice Bran Extract 1000 g of purified water was mixed with 330 g of germinated brown rice bran, extracted at 30 ° C. for 24 hours, and then filtered to obtain 245 g of a light brown transparent extract solution (solid content 4) .21%). This was diluted with purified water so that the solid content concentration would be 1.50% to obtain an extract solution of germinated brown rice bran.

Production Example 3 Preparation of germinated brown rice bran extract 100 g of germinated brown rice bran mixed with 1000 g of 8: 2 (weight ratio) mixture of purified water and ethanol, extracted at 4 ° C. for 72 hours, filtered, and germinated in a light brown transparent 650 g of brown rice bran extract solution was obtained (solids concentration 1.44%).

Production Example 4 Preparation of germinated brown rice koji extract 200 g of germinated brown rice koji is mixed with 1200 g of a 7: 3 (weight ratio) mixture of purified water and 1,3-butylene glycol, filtered at 80 ° C. for 2 hours, filtered. 820 g of a light brown transparent germinated brown rice koji extract solution was obtained (solid content concentration 1.25%).

Production Example 5 Preparation of germinated brown rice bran extract 500 g of a germinated brown rice bran extract solution prepared in the same manner as in Production Example 1 was lyophilized and pulverized to obtain 7.0 g of yellow brown germinated brown rice bran extract powder.

Production Example 6 Preparation of Enzymatic Decomposition Solution of Germinated Brown Rice Koji Extract 100 g of Germinated Brown Rice Koji was mixed with 1000 g of purified water, extracted at 50 ° C. for 3 hours and then filtered to obtain 340 g of a germinated brown rice koji extract solution. . To this solution, 0.5 g of lipase was added, hydrolyzed at 50 ° C. for 3 hours, then heated at 80 ° C. for 1 hour to inactivate the enzyme, filtered, and light brown transparent germination 301 g of enzyme degradation product solution of brown rice bran extract was obtained (solid content concentration 2.11%).

Production Example 7 Preparation of Enzymatic Decomposition Solution of Germinated Brown Rice Koji Extract 100 g of Germinated Brown Rice Koji was mixed with 1000 g of purified water, extracted at 50 ° C. for 3 hours and then filtered to obtain 340 g of a germinated brown rice koji extract solution. . After adding 0.1 g each of lipase, papain, glucoamylase and cellulase to this solution, hydrolyzing at 50 ° C. for 3 hours, and then heating at 80 ° C. for 1 hour to deactivate the enzyme As a result, 311 g of an enzyme degradation product solution of a light brown transparent germinated brown rice bran extract was obtained (solid content concentration 1.90%).

Production Example 8 Preparation of fermented lactic acid bacteria solution of germinated brown rice bran extract Mix 100 g of germinated brown rice bran with 900 g of purified water, extract at 80 ° C. for 2 hours, and filter to obtain about 500 g of a pale yellow transparent extract solution. (Solid content concentration 3.87%). The obtained extract solution was sterilized by heating. After adding 0.2 g of lipase, 0.2 g of papain, 0.2 g of glucoamylase and 0.2 g of pectinase to this extract solution, inoculate 10 ⁸ cells / mL of lactic acid bacteria (Lactobacillus plantarum) under nitrogen stream. The culture was allowed to stand at 37 ° C. for 18 hours. After culturing, the mixture was sterilized by heating, and the culture solution was filtered and decolorized to obtain 322 g (solid content concentration: 3.67%) of a fermented lactic acid bacterium solution of germinated brown rice bran extract.

Production Example 9 Preparation of a koji mold fermented solution of germinated brown rice koji extract A koji mold fermented solution of germinated brown rice koji extract as in Production Example 8 except that koji mold (Aspergillus oryzae) is used instead of lactic acid bacteria as the fungus used for fermentation 330 g (solid content concentration 3.69%) was obtained.

Production Example 10 Preparation of fermented yeast solution of germinated brown rice koji extract Same as in Production Example 8 except that lactic acid bacteria are used instead of lactic acid bacteria as a bacterium used for fermentation (however, the culture temperature is 30 ° C.) 380 g (solid content concentration 3.22%) of yeast fermented product solution of koji extract was obtained.

Production Example 11 Preparation of fermented natto fermented solution of germinated brown rice koji extract Natto fungus of germinated brown rice koji extract in the same manner as in Production Example 8, except that lactic acid bacteria were used as the fungus used for fermentation. 312 g of fermented product solution (solid content concentration 3.59%) was obtained.

Production Example 12. Preparation of Tempe Bacteria Fermented Product Solution of Germinated Brown Rice Bran Extract Similar to Production Example 8, except for using Tempe Bacteria (Rhizopus microsporus oligospora) instead of Aspergillus as the fungus used for fermentation, 392 g (solid content concentration 3.18%) of a Tempe fungus fermentation product solution was obtained.

Production Example 13 Preparation of fermented lactic acid bacteria solution of germinated brown rice bran extract 332g of fermented lactic acid bacteria solution of germinated brown rice bran extract in the same manner as in Production Example 8 except that no enzyme was added and the fermentation period was 3 days. (Solid content concentration 3.44%) was obtained.

Production Example 14 Preparation of a koji mold fermented solution of germinated brown rice koji extract 339 g of a koji mold fermented solution of sprouted brown rice koji extract in the same manner as in Production Example 9 except that no enzyme was added and the fermentation period was 3 days. (Solid content concentration 3.22%) was obtained.

Production Example 15. Preparation of yeast fermented solution of germinated brown rice koji extract 360 g of yeast fermented solution of germinated brown rice koji extract in the same manner as in Production Example 10 except that no enzyme was added and the fermentation period was 3 days. (Solid content concentration 3.36%) was obtained.

Production Example 16. Preparation of fermented natto bacteria fermented solution of germinated brown rice bran extract In the same manner as in Production Example 11, except that no enzyme was added and the fermentation period was 3 days, fermented natto fungus of germinated brown rice bran extract 382 g of solution (solid content concentration 3.45%) was obtained.

Production Example 17 Preparation of fermented tempeh fungus solution of germinated brown rice pod extract 322 g of fermented solution of germinated brown rice bud extract in the same manner as in Production Example 12 except that no enzyme was added and the fermentation period was 3 days. (Solid content 3.39%) was obtained.

Production Example 18. Preparation of lactic acid bacteria fermented product solution of germinated brown rice pod extract Lactobacillus fermented product of germinated brown rice bud extract in the same manner as in Production Example 8 except that Lactobacillus plantarum is used as lactic acid bacteria instead of Lactobacillus plantarum 304 g of solution (solid content concentration 3.82%) was obtained.

Production Example 19. Preparation of lactic acid bacteria fermented product solution of germinated brown rice pod extract 310 g of lactic acid bacteria fermented solution of germinated brown rice bud extract in the same manner as in Production Example 8 except that Leuconostoc mesenteroids is used instead of Lactobacillus plantarum as lactic acid bacteria Solid content concentration 3.61%) was obtained.

Production Example 20. Preparation of fermented lactic acid bacteria solution of germinated brown rice pods A suspension was prepared by mixing 900 g of purified water with 100 g of germinated brown rice buds, and sterilized by heating. After adding 1.0 g of lipase, 1.0 g of papain, 1.0 g of glucoamylase and 1.0 g of pectinase to this suspension, inoculate 10 ⁸ cells / mL of lactic acid bacteria (Lactobacillus plantarum) under nitrogen stream. The culture was stationary at 37 ° C. for 3 days. After completion of the culture, the mixture was sterilized by heating, the culture solution was filtered, and decolorization treatment was performed to obtain 632 g (solid content concentration: 4.12%) of a lactic acid bacteria fermentation product of germinated brown rice bran.

Production Example 21. Preparation of yeast fermented solution of germinated brown rice bran (2)
A suspension was prepared by mixing 900 g of purified water with 100 g of germinated brown rice straw and sterilized by heating. To this suspension, 1.0 g of lipase, 1.0 g of papain, 1.0 g of glucoamylase and 1.0 g of pectinase were added, followed by inoculation of 10 ⁸ cells / mL of yeast (Saccharomyces cerevisiae), and the mixture was allowed to stand at 30 ° C. for 3 days. Incubated. After culturing, the mixture was sterilized by heating, the culture solution was filtered, and decolorization treatment was performed to obtain 522 g (solid content concentration 3.99%) of a fermented brown rice bran yeast fermentation product.

Production Example 22. Preparation of fermented lactic acid bacteria powder of germinated brown rice bran extract 300 g of lactic acid bacteria fermented solution of germinated brown rice bran extract obtained in the same manner as in Production Example 8 was freeze-dried and ground to extract germinated brown rice bran extract 12 g of lactic acid bacteria fermented powder was obtained.

Production Example 23. Preparation of germinated brown rice koji extract powder of germinated brown rice koji extract 300 g of a germinated brown rice koji extract solution obtained in the same manner as in Production Example 9 was freeze-dried and pulverized to extract germinated brown rice koji extract 11 g of a koji mold powder was obtained.

Production Example 24. Preparation of fermented yeast rice powder of germinated brown rice pod extract 300 g yeast fermented yeast solution of germinated brown rice bud extract obtained in the same manner as in Production Example 10 was freeze-dried and pulverized to extract germinated brown rice potato extract 10 g of yeast fermented product powder was obtained.

Production Example 25. Preparation of fermented natto bacteria fermented powder of germinated brown rice koji extract 300 g of fermented natto fungus solution of germinated brown rice koji extract obtained in the same manner as in Production Example 11 was freeze-dried, pulverized, and germinated brown rice koji 11 g of fermented natto fermented powder was obtained.

Production Example 26. Preparation of tempeh bacteria fermented powder of germinated brown rice koji extract 300 g of sprouting brown rice koji extract solution of sprouting brown rice koji extract obtained in the same manner as in Production Example 12 was freeze-dried and pulverized to give germinated brown rice koji 11 g of a tempe fungus fermentation powder was obtained.

Comparative Production Example 1 Preparation of rice bran extract In Production Example 1, a rice bran extract solution was obtained in the same manner as in Production Example 1 except that rice germ derived from non-germinated ordinary rice was used instead of germinated brown rice. 60%).

Comparative Production Example 2 Preparation of fermented lactic acid bacteria solution of rice bran extract.
In Production Example 8, a lactic acid bacteria fermented solution of rice bran extract was obtained in the same manner as in Production Example 8, except that instead of germinated brown rice koji, rice germ derived from non-germinated ordinary rice was used. (Solid content 3.96%).

Example 1. Cream [Component A] Liquid paraffin 5.0
Hexalan (Note 1) 4.0
Paraffin 5.0
Glyceryl monostearate 2.0
Polyoxyethylene (20) sorbitan monostearate 6.0
Butylparaben 0.1
(Note 1) Technoble Co., Ltd. glyceryl trioctanoate
[B component]
Germination brown rice bran extract solution of Production Example 1 10.0
Glycerin 5.0
Methylparaben 0.1
Moiston C (Note 2) 1.0
Amount of purified water totaling 100 parts
(Note 2) NMF component manufactured by Technoble Co., Ltd.
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After this was cooled to 50 ° C., component C was added and further stirred and mixed to obtain a cream.

Example 2 Cream A cream was obtained in the same manner as in Example 1 except that the germinated brown rice bran extract solution of Production Example 1 was used instead of the germinated brown rice bran extract solution of Production Example 1 in the component B of Example 1.

Example 3 Cream A cream was obtained in the same manner as in Example 1 except that the germinated brown rice bran extract solution of Production Example 1 was used instead of the germinated brown rice bran extract solution of Production Example 1 in the component B of Example 1.

Example 4 Cream In the same manner as in Example 1, except that the lactic acid bacteria fermented solution of the germinated brown rice bran extract of Production Example 8 is used instead of the germinated brown rice bran extract solution of Production Example 1 in the component B of Example 1 Got.

Example 5 FIG. Cream In the same manner as in Example 1, except that the B component of Example 1 was replaced with the germinated brown rice bran extract solution of Production Example 1 and the fermented natto fermentation solution of germinated brown rice bran extract of Production Example 11 was used. A cream was obtained.

Example 6 Cream In the same manner as in Example 1, except that the fermented brown rice bran extract solution of Production Example 10 was used instead of the germinated brown rice bran extract solution of Production Example 1 in the component B of Example 1. Got.

Example 7 Cream In the same manner as in Example 1, except that the tempered fungus solution of the germinated brown rice bran extract of Production Example 12 was used in the component B of Example 1 instead of the germinated brown rice bran extract solution of Production Example 1. A cream was obtained.

Example 8 FIG. Cream In the same manner as in Example 1, except that in the component B of Example 1, the germinated brown rice bran extract solution of Production Example 1 was used instead of the germinated brown rice bran extract of Production Example 18 A cream was obtained.

Example 9 Cream In the same manner as in Example 1, except that the enzyme decomposed product solution of the germinated brown rice bran extract of Production Example 6 was used in the component B of Example 1 instead of the germinated brown rice bran extract solution of Production Example 1 Got.

Example 10 Cream In the same manner as in Example 1 except that the enzyme decomposed product solution of germinated brown rice bran extract of Production Example 7 was used in the component B of Example 1 instead of the germinated brown rice bran extract solution of Production Example 1 Got.

Example 11 Cream In the component B of Example 1, a cream was obtained in the same manner as in Example 1 except that the germinated brown rice bran lactic acid bacteria fermented solution of Production Example 20 was used instead of the germinated brown rice bran extract solution of Production Example 1. It was.

Example 12 Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monostearate 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Germination brown rice bran extract solution of Production Example 1 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Amount of purified water totaling 100 parts
[C component]
Perfume
The components A and B were each heated to 80 ° C. or higher and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 13 Lotion [Ingredients] part Bacteria fermented fermented solution of germinated brown rice bran extract of Production Example 10.0 10.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Perfume
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total
The above ingredients were mixed to obtain a lotion.

Example 14 Lotion [component A] part olive oil 1.0
Polyoxyethylene (5.5) cetyl ether 0.5
Butylparaben 0.1
[B component]
Tempered Bacteria Fermentation Solution of Germinated Brown Rice Bran Extract of Production Example 12 10.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Methylparaben 0.1
Potassium hydroxide appropriate amount Purified water Amount that makes 100 parts in total
[C component]
Perfume
After each component A and component B was heated to 80 ° C. or higher, the component B was added to the component A and stirred, and further homogenized with Hiscotron (5000 rpm) for 2 minutes.
After cooling this to 50 degreeC, C component was added and stirred and mixed, and also it cooled to 30 degrees C or less, and the lotion was obtained.

Example 15. Emulsion Emulsion is obtained in the same manner as in Example 12 except that the fermented brown rice bran extract solution of Production Example 21 is used in the component B of Example 12 instead of the germinated brown rice bran extract solution of Production Example 1. It was.

Example 16 Emulsion In the same manner as in Example 12, except that the germinated brown rice bran extract solution of Production Example 13 was used in the component B of Example 12 instead of the germinated brown rice bran extract solution of Production Example 13. Got.

Example 17. Emulsion In the same manner as in Example 12, except that the germinated brown rice koji extract solution of Production Example 14 was used instead of the germinated brown rice koji extract solution of Production Example 1 in the component B of Example 12. Got.

Example 18 Emulsion Emulsion in the same manner as in Example 12 except that the fermented brown rice bran extract solution of Production Example 15 was used instead of the fermented brown rice bran extract solution of Production Example 1 in the component B of Example 12. Got.

Example 19. Emulsion In the component B of Example 12, the milk solution was prepared in the same manner as in Example 12, except that the germinated brown rice bran natto fermentation solution of Production Example 16 was used instead of the germinated brown rice bran extract solution of Production Example 1. Obtained.

Example 20. Emulsion [component A] part liquid paraffin 6.0
Hexalan 4.0
Jojoba oil 1.0
Polyoxyethylene (20) sorbitan monosteare 2.0
Soy lecithin 1.5
Methylparaben 0.15
Ethylparaben 0.03
[B component]
Germination brown rice bran extract solution of Production Example 1 10.0
L-ascorbic acid-2-glucoside 2.0
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethylcellulose 0.3
Sodium hyaluronate 0.01
Potassium hydroxide Appropriate amount Purified water Amount that makes the total amount 100 parts [Component C]
Fragrance Appropriate amount Each of the above components A and B was heated to 80 ° C. or higher, and then mixed by stirring. After cooling this to 50 ° C., component C was added and further stirred and mixed to obtain an emulsion.

Example 21. Emulsion In addition to using 2.0 parts of L-ascorbic acid-2-phosphoside magnesium in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide in the component B of Example 20 Obtained an emulsion in the same manner as in Example 20.

Example 22. Emulsion In addition to using 2.0 parts of L-ascorbic acid-2-phosphate sodium salt in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the component B of Example 20 Obtained an emulsion in the same manner as in Example 20.

Example 23. Emulsion In the component B of Example 20, the emulsion was prepared in the same manner as in Example 20 except that 2.0 parts of arbutin was used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide. Obtained.

Example 24. Latex In the component B of Example 20, in place of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide, a rice bran extract hydrolyzate (trade name “Grace Snow” manufactured by Technoble Co., Ltd.) An emulsion was obtained in the same manner as in Example 20 except that 5.0 parts of * Snow * AE ", solid concentration 3.5%) were used.

Example 25. Latex In the component B of Example 20, instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide, white seed (Brassica Alba) seed extract (trade name, manufactured by Technoble Co., Ltd.) An emulsion was obtained in the same manner as in Example 20 except that 5.0 parts of “Sinablanca-WH” (solid content concentration 1.0%) were used.

Example 26. Emulsion Example 20 except for using 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide in the component B of Example 20 except that 1.0 part of γ-amino-β-hydroxybutyric acid is used. In the same manner as in No. 20, an emulsion was obtained.

Example 27. Latex In the component B of Example 20, instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide, a rice extract hydrolyzate (trade name “Orisenoble, manufactured by Technoble Co., Ltd.) A solid emulsion of 1.5% was obtained in the same manner as in Example 20 except that 5.0 parts were used.

Example 28. Lotion [Ingredient] part Germinated brown rice bran extract solution of Production Example 1 10.0
L-ascorbic acid-2-glucoside 2.0
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Citric acid 0.1
Sodium citrate 0.3
Sodium alginate 0.1
Potassium hydroxide Appropriate amount Perfume Appropriate amount Purified water An amount that makes the total amount 100 parts The above ingredients were mixed to obtain a lotion.

Example 29. Pressed powder [A component part Bengala 0.5
Yellow iron oxide 1.5
Black iron oxide 0.1
Titanium oxide 10.0
6-Nylon powder 4.0
Amount that makes 100 parts of sericite Mica 23.0
Talc 25.0
Germinated brown rice bran extract powder of Production Example 5 0.2
[B component]
Squalane 1.0
Methylpolysiloxane 4.0
Propylparaben 0.1
Dehydroacetic acid 0.1
Liquid paraffin 2.0
Perfume
The above A component and B component were mixed and agitated, mixed, then passed through a 200 mesh Tyler mesh sieve, and the resulting mixed powder was cast into a mold to obtain a pressed powder.

Example 30. FIG. Liquid foundation [component A] part Stearic acid 2.4
Propylene glycol monostearate 2.0
Cetostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
[B component]
Tempe Fungus Fermented Solution of Germinated Brown Rice Bran Extract of Production Example 12 5.0
Sodium carboxymethylcellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Purified water Amount that makes the total amount 100 parts [C component]
Titanium oxide 8.0
Talc 4.0
Coloring pigment appropriate amount The components A and B were heated and mixed and stirred. This was reheated, the above C component was added, poured into a mold, and stirred until it reached room temperature to obtain a liquid foundation.

Example 31. Cream foundation [component A] part Stearic acid 5.0
Cetanol 2.0
Glyceryl monostearate 3.0
Liquid paraffin 5.0
Squalane 3.0
Isopropyl myristate 8.0
Polyoxyethylene (20) glyceryl monostearate 2.0
Propylparaben 0.1
[B component]
Germinated brown rice bran extract solution of Production Example 1 5.0
Sorbitol 3.0
1,3-butylene glycol 5.0
Triethanolamine 1.5
Methylparaben 0.1
Purified water Amount of 100 parts [component C]
Titanium oxide 8.0
Talc 2.0
Kaolin 5.0
Bentonite 1.0
Coloring pigment appropriate amount [component D]
Fragrance 0.3
Component C was mixed and pulverized with a pulverizer. The component B was mixed, and the pulverized component C was added thereto and uniformly dispersed in a colloid mill. The components A and B and C dispersed uniformly were each heated to 80 ° C., and then the components A were added to the components B and C while stirring, and further homogenized with Hiscotron (5000 rpm) for 2 minutes. After cooling this to 50 degreeC, D component was added and stirred and mixed, and also it cooled to 30 degrees C or less, stirring, and obtained the cream foundation.

Example 32. Hair artic [component A] part ethanol 60.0
O-methylcephalanoline 0.002
l-Menthol 0.5
Fragrance 0.1
Methylparaben 0.1
[B component]
Glycerin 2.0
1,3-butylene glycol 2.0
Tempered Bacteria Fermented Solution of Germinated Brown Rice Bran Extract of Production Example 17 10.0
Purified water in an amount of 100 parts The above A component and B component were each dissolved at room temperature, then the B component was added to the A component with stirring and dissolved to obtain a hair-tonic.

Example 33. Hair treatment [ingredient] part stearyltrimethylammonium chloride 6.0
Polyvinylpyrrolidone 4.0
Glycerin 1.0
Ethylparaben 0.1
Lactic acid bacteria fermented solution of germinated brown rice bran extract of Production Example 8 5.0
Purified water in a total amount of 100 parts The above ingredients were heated to 80 ° C and then mixed and stirred to obtain a hair treatment. This product was also suitable as a hair pack.

Example 34. Hair shampoo [component A] part N-coconut oil fatty acid methyl taurine sodium 10.0
Polyoxyethylene (3) sodium alkyl ether sulfate 20.0
Lauryldimethylaminoacetic acid betaine 10.0
Palm oil fatty acid diethanolamide 4.0
Methylparaben 0.1
[B component]
Citric acid 0.1
Germinated brown rice bran extract solution of Production Example 1 5.0
1,3-butylene glycol 2.0
Purified water Amounts of 100 parts in total A and B components were each heated to 80 ° C and dissolved uniformly, then B component was added to A component and stirring continued to cool to room temperature to obtain a hair shampoo It was.

Example 35. Hair rinse [component A] Part Polyoxyethylene (10) hydrogenated castor oil 1.0
Distearyldimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
[B component]
Lactic acid bacteria fermented product solution of germinated brown rice bran extract of Production Example 19 5.0
1,3-butylene glycol 5.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain hair rinse It was.

Example 36. Body shampoo [component A] part N-lauroylmethylalanine sodium 25.0
Palm oil fatty acid potassium liquid (40%) 26.0
Palm oil fatty acid diethanolamide 3.0
Methylparaben 0.1
[B component]
Germination brown rice bran extract solution of Production Example 1 10.0
1,3-butylene glycol 2.0
Purified water Amount to be 100 parts A component and B component are each heated to 80 ° C and dissolved uniformly, then B component is added to A component and stirring is continued to cool to room temperature to obtain a body shampoo It was.

Example 37. Soap [component A] part hardened castor oil 26.0
Coconut oil 10.0
Olive oil 4.0
[B component]
Sodium hydroxide 6.0
Sugar 10.0
Glycerin 5.0
Fermented lactic acid bacteria powder of germinated brown rice bran extract of Production Example 22 0.5
Purified water Amount of 100 parts [component C]
Ethanol 20.0
Fragrance Appropriate amounts of A component and B component were each heated to 80 ° C. and dissolved uniformly, and then B component was added to A component to saponify. This was cooled to 50 ° C. with stirring, and component C was added. This was poured into a mold and cooled, and then dried at room temperature for several days. A sufficiently dried product was taken out from the mold to obtain soap.

Comparative Example 1 Cream In the component B of Example 1, a cream was obtained in the same manner as in Example 1 except that instead of the germinated brown rice koji extract solution of Production Example 1, the koji extract solution derived from plain rice of Comparative Production Example 1 was used. It was.

Comparative Example 2 Cream In the same manner as in Example 1 except that, in the component B of Example 1, the sprouting brown rice koji extract solution of Production Example 1 was used instead of the common rice-derived koji extract of Comparative Production Example 2. A cream was obtained.

Test Example 1 Fibroblast activation test Germinated brown rice bran extract obtained in Production Examples 1 to 3, 6 and 8 to 12, its enzyme-treated product solution and its fermented product solution (invention example) and Comparative Production Example 1 and The solution of the ordinary rice bran extract obtained in 2 and its fermented product solution (hereinafter referred to as Comparative Example) as a sample and diluted with purified water to adjust the solid content to 1%. The enhancing effect on blast activity was examined.

[Test method]
Human skin dermis-derived fibroblasts NB1RGB are seeded at 1 × 10 ⁴ cells / well in a 96-well microplate containing 0.5% NCS-containing minimum essential medium, pre-cultured at 37 ° C. for 1 day, and then sampled in the medium The solution was added to a concentration of 2.5 or 5.0% (as a solution concentration) and cultured at 37 ° C. for another 3 days. Next, 0.2% of MTT was added to the cell treatment solution to which the medium was removed and the surfactant (TRITON X-100) was added and maintained at 37 ° C., and then MTT was reduced at a wavelength of 370 to 630 nm. The formazan produced was measured. Moreover, in order to confirm whether the test system is functioning normally, the same test was performed when 100 mM of glucose was added instead of the test sample and when no sample was added (control).
The fibroblast activation effect of each sample was shown by absorbance (MTT activity) at a wavelength of 370 to 630 nm.

[result]
The results are shown in Table 1. In Table 1, the MTT activity rate represents the relative value of the MTT activity when each sample was added, where the MTT activity when no sample was added (control) was 100.
As shown in Table 1, in the germinated brown rice bran extract of the present invention (Production Examples 1 to 3), an effect of enhancing MTT activity (cell activity) with respect to normal human-derived fibroblasts was confirmed. . In addition, the enzyme degradation product (Production Example 6) and fermented product (Production Examples 8 to 12) of germinated brown rice koji extract showed a strong cell activity enhancing effect similar to that of the extract.
In contrast, the extract of common rice bran extracted as a comparative example (Comparative Production Example 1) and its fermented product (Comparative Production Example 2) also showed a slight activation of fibroblasts. The action was much weaker than that of the sample of the present invention.

Test Example 2 Collagen production promotion test Collagen production promotion action was examined for the same sample as in Test Example 1 (solid content concentration 1.0%).
[Test method]
Human skin dermis-derived fibroblasts NB1RGB are seeded at 1 × 10 ⁴ cells / well in a 96-well microplate containing 0.5% NCS-containing minimum essential medium, pre-cultured at 37 ° C. for 1 day, and then sampled in the medium The solution was added to a concentration of 2.5 or 5.0% (as a solution concentration) and cultured at 37 ° C. for another 5 days.
Next, the culture solution was removed, 0.04% fast green, 0.05% sirius red solution was added, and intracellular collagen and non-collagen were stained, and then washed thoroughly. Next, extraction was performed with 0.1% NaOH-containing methanol, and the amount of collagen was measured at a wavelength of 540 nm. Moreover, in order to confirm whether the test system is functioning normally, when 2 mM of L-ascorbic acid-2-phosphate magnesium (APM) is added instead of the test sample and when no sample is added (control) A similar test was conducted.
The results are shown as relative values (collagen synthesis rate) of the absorbance when each sample was added when the absorbance at a wavelength of 520 nm in the case where no sample was added (control) was taken as 100.

[result]
The results are shown in Table 2.

As shown in Table 2, the effect of significantly enhancing collagen production in normal human-derived fibroblasts was confirmed in the germinated brown rice koji extract of the present invention (Production Examples 1 to 3). In addition, the enzyme degradation product (Production Example 6) and fermented product (Production Examples 8 to 12) of germinated brown rice koji extract showed the same collagen synthesis promoting action as the above extract.
On the other hand, the collagen synthesis promoting action of ordinary rice bran extract (Comparative Production Example 1) and fermented product (Comparative Production Example 2) was far from that of the sample of the present invention.

Test Example 3 Skin irritation [sample]
(1) The kneaded extract of Production Example 1 in JP Hydrophilic Petrolatum to a concentration of 5% (Invention Sample 1)
(2) Kneaded extract of Production Example 2 to JP Hydrophilic Vaseline to a concentration of 5% (Invention Sample 2)
(3) A product obtained by kneading the enzyme degradation product solution of Production Example 6 with JP Vaseline to a concentration of 5% (Invention Sample 3)
(4) Kneaded fermented product solution of Production Example 8 in JP Hydrophilic Petrolatum to a concentration of 5% (Invention Sample 4)
(5) Kneaded fermented product solution of Production Example 12 in JP Hydrophilic Petrolatum to a concentration of 5% (Invention Sample 5)
(6) JP Hydrophilic Petrolatum (control)

[Test method]
Five adult males aged 20 to 50 years were used as subjects, and the inner side of each upper arm was wiped with ethanol to remove sebum. What applied each 0.2g of hydrophilic petrolatum was stuck. After 24 hours, the fin chamber was removed, and the degree of skin irritation was determined by the method and criteria described below.
[Judgment]
After 1 hour, 24 hours and 48 hours after removing the patch, the state of erythema and edema at the applied site was visually determined based on the following “skin irritation criteria by dreze method”, and the average value of 5 subjects Asked.
(Erythema)
Score Skin condition 0: No erythema 1: Extremely mild erythema 2: Clear erythema 3: Moderate to strong erythema 4: Light crust formation in strong crimson erythema (edema)
Score Skin condition 0: No edema 1: Extremely mild edema 2: Clear edema (clearly distinguishable from surroundings)
3: Moderate edema (swelling of 1 mm or more)
4: Strong edema (further spread around)

The results are shown in Table 3.

  As apparent from the results in Table 3, the germinated brown rice bran extract of the present invention, its enzymatic degradation product and its fermented product have no skin irritation and are extremely safe.

Test Example 1 Monitor Test The effects of the creams of Examples 1 and 4 and the creams of Comparative Examples 1 and 2 on the skin were examined by a monitor test.
[Test method]
Randomly extracted 40 women aged 18-50 were divided into two groups (A, B) of 20 subjects as subjects, and each group was divided into Example 1 and Comparative Example 1 cream or Example 4. One of the two combinations of the cream of Comparative Example 2 was allocated, and the cream of the Example or Comparative Example was applied to the left and right cheeks twice a day (morning and evening) for one month. Then, the improvement effect with respect to fine wrinkles and the improvement effect with respect to skin tension and gloss were evaluated based on the following evaluation criteria.

[Evaluation criteria]
(Improvement effect on small wrinkles)
A: Almost inconspicuous B: Almost inconspicuous C: Slightly inconspicuous D: Unchanged E: On the contrary (Improvement effect on skin elasticity and gloss)
A: Clearly improved B: Much improved C: Slightly improved D: No change E: On the contrary

[result]
The results are shown in Table 4. In addition, the number of each evaluation column of AE in a table | surface shows the number of the test subjects who performed the said evaluation in 20 test subjects.

As shown in Table 4, the cream of the present invention comprising an extract of germinated brown rice bran extract or a fermented product thereof as an active ingredient prevents wrinkles, sagging, etc., which are typical symptoms of aging or unhealthy skin. Excellent improvement effect.
On the other hand, in the cream of the comparative example which mix | blended the extract of the common rice bran, or its fermented product, the improvement effect was hardly seen but it resulted in a difference in effectiveness clearly.

Claims (3)

A cosmetic comprising a germ obtained by milling germinated brown rice into germinated rice, and a koji extract mainly composed of an envelope of endosperm . A rice bran or its extract, which is mainly composed of germ and endosperm shell obtained by milling germinated brown rice into a germinated rice, is obtained from lipase, a proteolytic enzyme, starch degrading enzyme, fibrinolytic enzyme, and lipolytic enzyme. A cosmetic comprising an enzyme degradation product obtained by treatment with at least one selected enzyme. A rice bran or extract thereof mainly composed of germ and endosperm shell obtained by polishing germinated brown rice into a germinated rice is at least one selected from lactic acid bacteria, koji molds, yeast, natto bacteria, and tempeh bacteria. A cosmetic comprising a fermented product obtained by fermentation.