KR20180064185A - Process for producing fermented rice bran lactic acid bacteria fermentation liquid and The cosmetic composition containing the same - Google Patents

Abstract

According to an embodiment of the present invention, a cosmetic composition containing a lactobacillus fermentation solution is able to include a lactobacillus fermentation solution made by fermenting a mixture extract, containing a rice bran extract, a soybean extract, a scutellaria extract, a green tea extract, a dried mugwort extract, a Houttuynia cordata extract, an Aloe vera extract, a milk vetch extract, a forsythia fruit extract, a Rehmannia extract, an Acanthopanax extract, a Centella extract, a G. sinensis extract, a phellodendron bark extract, a Reynoutria extract, a hedge parsley extract, a dittany extract, a ginger extract, and an oat extract, with lactobacillus. The lactobacillus fermentation solution manufacturing method includes: a step (a) of obtaining precipitates of rice bran, soybean, Scutellaria, green tea, dried mugwort, Houttuynia cordata, aloe vera, milk vetch, forsythia fruit, Rehmannia, Acanthopanax, Centella, G. sinensis, phellodendron bark, Reynoutria, hedge parsley, dittany, ginger, and oats; a step (b) of mixing all the plants of the step (a) as 1-2 parts by weight and smashing the mixture; a step (c) of extracting the mixture of the step (b) at 40-80°C with a pressure of 300-500 bars by using a boiling machine; a step (d) of obtaining a mixture extract by concentrating the extract of the step (c); and a step (e) of obtaining a lactobacillus fermentation solution by treating the mixture of the step (d) with lactobacillus.

Description

TECHNICAL FIELD The present invention relates to a process for producing fermented rice bran fermented rice bran and a cosmetic composition containing the fermented bran fermented rice bran fermented by the above method,

The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition containing lactobacillus fermented with rice bran.

Lactic acid bacteria, lactic acid bacteria, also called lactic acid bacteria, are collectively referred to as bacteria on the back wall fungus which break down carbohydrates into lactic acid by metabolism.

Lactic acid bacteria ferment foods using yogurt, yogurt, cheese and other dairy products, and bacteriological products such as sake, soybean paste, and soy sauce, and kimchi, which are inhibited by growth of pathogenic bacteria and harmful bacteria by lactic acid. In the digestive system or vagina, it protects the body from other hospital microorganisms and helps to maintain homeostasis.

In addition, lactic acid bacteria have a function to kill or inhibit proliferation of the intestinal bacteria, improve immunity, improve nutritional value, improve cirrhosis, antitumor activity, and lower serum cholesterol level, and remove harmful bacteria and toxic substances, It inhibits the growth of folliculitis and has an effect on skin beauty.

The present invention provides a method for producing a lactic acid fermentation broth by fermenting a plant mixed extract with lactic acid bacteria and a cosmetic composition to which a lactic acid fermentation broth is added using the characteristics of the lactic acid bacterium as described above.

In order to achieve the above object, a cosmetic composition containing a lactic acid fermentation broth according to an embodiment of the present invention is characterized in that it comprises at least one selected from the group consisting of rice bran extract, soybean extract, golden extract, green tea extract, Extracts, Rhizome Extracts, Ogphi Extracts, Centella asiatica Extracts, Scratches Tree Spiny Extracts, Leaf Extracts, Leaf Extracts, Lamiaceae Extracts, Caspase Extracts, Flower Mint Leaf Extracts, Ginger Extracts and Oat Extracts were fermented with lactic acid bacteria A fermentation broth.

The lactic acid bacteria fermentation broth is characterized in that it is fermented in rice bran.

The lactic acid fermentation broth may be selected from the group consisting of basic cosmetic formulations, color cosmetic formulations, hair cosmetic formulations, detergent formulations, shampoo formulations and body cosmetic formulations.

The lactic acid fermentation broth is characterized by containing 0.01 to 10% by weight based on the total weight of the cosmetic composition.

Further, the mixed extract is characterized in that it is extracted at a temperature of 40 to 80 캜 at a pressure of 300 to 500 bar using a stewer.

Further, the lactic acid bacterium is characterized by being Lactobacillus sp.

Next, in the production method for producing the lactic acid fermentation broth,

a) Rice bran, bean, golden, green tea, ladybug, lily of the valley, Aroebera, Hwanggi, allium, persimmon, Obtaining by precipitation; b) mixing and pulverizing 1 to 2 parts by weight of the plants obtained in step a); c) extracting the mixture of step b) with a boiler at a temperature of 40 to 80 DEG C at a pressure of 300 to 500 bar; d) concentrating the extract extracted in step c) to obtain a mixed extract; e) treating the mixed extract of step d) with a lactic acid bacterium to obtain a lactic acid fermentation broth.

The lactic acid bacterium of step e) is characterized by being Lactobacillus sp.

The lactic acid bacterium of step e) is inoculated into an MRS medium, cultured in a shaking incubator, and subcultured.

Also, the lactic acid bacteria of step e) is characterized in that the initial number of bacteria is adjusted to 107 to 109 CFU / ml.

The lactic acid fermentation broth of step (e) is characterized by being subjected to primary to tertiary filtration.

The fermented lactic acid fermentation broth according to one embodiment of the present invention has the effect of improving sulfation, improving whitening, improving skin trouble, and improving wrinkles.

Brief Description of the Drawings Fig. 1 is a flow chart showing a flow of a production method for producing a lactic acid fermentation broth.

Hereinafter, the description of the present invention with reference to the drawings is not limited to a specific embodiment, and various transformations can be applied and various embodiments can be made. It is to be understood that the following description covers all changes, equivalents, and alternatives falling within the spirit and scope of the present invention.

In the following description, the terms first, second, and the like are used to describe various components and are not limited to their own meaning, and are used only for the purpose of distinguishing one component from another component.

Like reference numerals used throughout the specification denote like elements.

As used herein, the singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. It is also to be understood that the terms " comprising, "" comprising, "or" having ", and the like are intended to designate the presence of stated features, integers, And should not be construed to preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Hereinafter, the cosmetic composition to which the lactic acid fermentation broth according to one embodiment of the present invention is added will be described in detail.

The cosmetic composition to which the lactic acid fermentation broth according to an embodiment of the present invention is added may comprise a lactic acid fermentation broth.

In detail, the lactic acid fermentation broth can be produced by fermenting a mixed extract containing various plant extracts with lactic acid bacteria. In this case, various kinds of plants are selected from the group consisting of rice bran extract, soybean extract, golden extract, green tea extract, liana extract, aloe vera extract, aloe vera extract, hwanggi extract, 0.01 to 10 parts by weight of rice bran extract, 0.01 to 10 parts by weight of soybean extract, 0.01 to 10 parts by weight of golden extract, green tea extract, green tea extract, 0.01 to 10 parts by weight of a leaf extract, 0.01 to 10 parts by weight of a leaf extract, 0.01 to 10 parts by weight of a herb extract, 0.01 to 10 parts by weight of an aloe vera extract, 0.01 to 10 parts by weight of an extract of Angelica keiskei, 0.01 to 10 parts by weight of an extract of Ogaki extract, 0.01 to 10 parts by weight of an extract of Kishinev family, 0.01 to 10 parts by weight of an extract of Scutellaria baicalensis, 0.01 to 10 parts by weight of a yellowish white extract, 0.01 to 10 weight 0.01 to 10 parts by weight of an extract of casualties, 0.01 to 10 parts by weight of a flower mint leaf extract, 0.01 to 10 parts by weight of a ginger extract, and 0.01 to 10 parts by weight of an oat extract to form a mixed extract.

Rice bran, which is a crushed mixture of peel, seed coat, and acupuncture that occurs during the process of turning from brown rice to white rice, contains various nutrients such as vitamin A, vitamin B1, vitamin B6, vitamin E, iron, phosphorus and minerals And it is effective to prevent constipation and prevent aging by activating intestinal action by plant fiber.

Soy is not only rich in protein, vitamin B group, iron and calcium, but also isoflavone, nucleic acid, and lacitin, and has an excellent effect for preventing and treating cerebral hemorrhage and cerebral ischemic stroke. And it has the effect of improving the hair.

In addition, gold does not have poison and is used as fever, diuretic, branch, ejaculatory and anti-inflammatory drugs, bitter taste into the heart and lungs, and strength to cool the liver and lungs.

Green tea is a catechin that gives off a tasty flavor. It inhibits the rise of blood pressure and stops activity by attaching to the surface of cold virus. It acts to delay digestion and absorption of saccharide, preventing blood pressure rise and blood glucose level rise, Can be prevented.

The lashes contain calcium, fiber, vitamin A, vitamin B1, vitamin C, a lot of chlorophyll and fiber, and it strengthens stomach and intestines, so it is good for constipation and increases bacterial resistance.

Hwasungcho is rich in components such as flavonoids, quercitrin, myrcens, and lauric aldehyde, and is effective for antibacterial action, diuretic action and skin care.

Aloe Vera has a gel containing polysaccharides such as glucomannans and pectic acid, as well as many other organic and inorganic compounds, and is effective in wound healing.

Hwanggi contains calcium, phosphorus, sodium, magnesium, potassium, iron, zinc, protein, crude fat, dietary fiber, ash and various amino acids and has effect of antibacterial effect and adipocyte differentiation.

The allium contains matairesinolglucoside, arctiin, arctigenin, matalrecinodide and vitamin C to prevent oxidation, diuretic action, antibacterial action, antihistaminic action, etc. It is effective.

It contains chalalpol, mannitol, lehmanin, stachyose, glucose and various amino acids. It is effective for skin diseases because it contains ingredients such as alkaloids, flavonoids and carotenoids.

Ogaki is rich in sugars such as Eureuteroside A to G, glucose and galactose, a large amount of carotene, vitamins B1, B2, C and minerals, and contains glycosides such as sterols, coumarins, liginins, flavones, It stimulates sedation and hormone secretion.

As a result, it is possible to control the collagen synthesis and promote skin elasticity and health by containing asiaticoside, asiatic acid, and madecassic acid.

It is effective to treat skin diseases such as sculptor tree wisteria glycoside, phenol, amino acid, tannin and so on.

It contains berberine, palmatine, magnofloline, phellodendrein, yeteorizine, guanidine, candidi, menisperin, pelamide, amaresin, etc., And so on.

It contains antimicrobial agents such as polygonine, emodine, emodine monomethyl ether, chrysopanol, reonotrine, isoquercitrin, oxyanthraquinone derivative, vitamin C, tannin, chlorogenic acid, Action and anti-inflammatory action.

Lamiaceae, also called candles, contains triterpenoids, oleanolic acid, ursolic acid, rutin, hyperoside, caffeic acid, and the like.

In addition, casualties have a therapeutic effect on skin diseases. Flower mint leaves are sterilizing and astringent, and oats are good for nails and skin health.

Thus, the extract may be isolated from plants having the above-mentioned effects and then concentrated to form a mixed extract.

Extraction of each extract constituting the mixed extract can be carried out at a temperature of from 40 캜 to 80 캜 using a boiling pot, and can be extracted under a pressure of 300 bar to 500 bar.

The mixed extract can also be fermented with the added lactobacillus (Lactobacillus sp.) Lactic acid bacteria. At this time, a lactic acid fermentation broth is obtained by lactic acid bacterium treatment. The cosmetic composition to which the lactic acid fermentation broth according to one embodiment of the present invention is added can be prepared by containing the lactic acid fermentation broth obtained by the above-mentioned process. Hereinafter, a detailed description of the method for producing lactic acid bacteria fermentation broth will be given later.

On the other hand, the lactic acid bacteria fermentation broth can be formed by rice bran fermentation.

In detail, the fermentation yeast is constituted by rice bran, and the fermented yeast is added to the mixed extract to proceed fermentation, whereby the lactic acid fermentation broth can be formed.

The lactic acid fermentation broth may contain 0.01 to 10% by weight based on the total weight of the cosmetic composition. The fermentation broth of the lactic acid bacteria produced in such a weight ratio may be selected from any one of basic cosmetic formulations, color cosmetic formulations, hair cosmetic formulations, detergent formulations, shampoo formulations and body cosmetic formulations.

1, the lactic acid fermentation broth includes a precipitation step (S100), a plant mixing and pulverizing step (S110), a pressure extraction step (S120), an extract concentration step (S130), and a mixed extract lactic acid bacterium treatment step (S140) can do.

In detail, the settling step (S100) includes a step of forming a rice cake, a bean, a golden tea, a green tea, a lobster, a lobster, an aerobera, an emperor, It may be a step of obtaining peppermint leaves, ginger, and oats by precipitation. At this time, the precipitation can be performed at room temperature, and the temperature at room temperature can be 1 to 35 캜.

The plant mixed pulverization step (S110) may be a step of pulverizing two or more plants obtained by the precipitation. At this time, the weight of each plant may be 1 to 2, and the mixed plant may be pulverized into a powder form using a pulverizer.

The step of extracting pressure (S120) may be a step of obtaining the extract by using the ground powder in the pulverized powder. At this time, the temperature of the hot water tank may be 40 ° C to 80 ° C, and a pressure of 300 to 500 bar may be applied to separate the extract from the plant.

The extract concentrate step (S130) may be a step of concentrating the separated extract to obtain a mixed extract. The mixed extract prepared through the step of concentrating the extract (S130) may contain lactobacillus (Lactobacillus sp.) Lactic acid bacteria And the fermentation can be performed through the mixed extract lactic acid bacteria treatment step (S140).

The lactic acid fermentation broth produced by fermentation can be finally formed by filtering the impurities by primary to tertiary filtration.

On the other hand, the cultivation of the lactic acid bacteria contained in the mixed extract lactic acid bacteria treatment step (S140) can be carried out through subculture in an MRS medium, after initial culture in a shaking incubator, and through subculture. As a result, the optimum initial number of lactic acid bacteria constituting the fermentation broth of lactic acid bacteria according to an embodiment of the present invention can be adjusted to 107 ~ 109 CFU / ml.

The cosmetic composition containing the fermentation broth of lactic acid bacterium prepared by the above method is unexpected in the effects of improving sulfation, improving whitening, improving skin troubles and improving wrinkles, and thus contains basic cosmetics, color cosmetics, hair cosmetics, detergents, shampoos and body cosmetics It can be added in any form of cosmetics.

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the scope of the present invention is not limited to the following examples.

[Example 1] Preparation of lactic acid fermentation broth fermented with rice bran

White rice, green bean, green tea, ladybug, aloe vera, hwanggi, allium, rhubarb, oggie, bilberry, Followed by pulverization. Then, the plant extract was extracted at a pressure of 400 bar at a temperature of 70 캜 using a hot water bath. The mixture was concentrated to prepare a mixed extract.

Lactobacillus sp. Lactobacillus was inoculated into MRS medium, cultured in a shaking incubator, subcultured, and initial bacterial counts were adjusted to 107 ~ 109 CFU / ml.

Lactobacillus sp. Lactobacillus cultured on the mixed extract was treated and then subjected to tertiary filtration on a filter paper to prepare a lactic acid fermentation broth.

[Comparative Example 1] Production of lactic acid fermentation broth fermented with mushroom

Mixed with the same amount of rice bran, bean, golden, green tea, ladybug, aloe vera, aloe vera, hwanggi, allium, rhubarb, ogapi, And the plant extract was extracted at a pressure of 400 bar at a temperature of 70 캜 using a hot water bath. The mixture was concentrated to prepare a mixed extract.

Lactobacillus sp. Lactobacillus was inoculated into MRS medium, cultured in a shaking incubator, subcultured, and initial bacterial counts were adjusted to 107 ~ 109 CFU / ml.

Lactobacillus sp. Lactobacillus cultured on the mixed extract was treated, followed by fermentation with mushroom, followed by tertiary filtration on a filter paper to prepare a lactic acid fermentation broth.

[Comparative Example 2] Production of lactic acid fermentation broth fermented with pomegranate

Mixed with the same amount of rice bran, bean, golden, green tea, ladybug, aloe vera, aloe vera, hwanggi, allium, rhubarb, ogapi, And the plant extract was extracted at a pressure of 400 bar at a temperature of 70 캜 using a hot water bath. The mixture was concentrated to prepare a mixed extract.

Lactobacillus sp. Lactobacillus was inoculated into MRS medium, cultured in a shaking incubator, subcultured, and initial bacterial counts were adjusted to 107 ~ 109 CFU / ml.

Lactobacillus sp. Lactobacillus cultured on the mixed extract was treated, followed by fermentation with pomegranate, followed by tertiary filtration on a filter paper to prepare a lactic acid fermentation broth.

[Comparative Example 3] Production of fermentation broth without lactic acid bacteria

Mixed with the same amount of rice bran, bean, golden, green tea, ladybug, aloe vera, aloe vera, hwanggi, allium, rhubarb, ogapi, And the plant extract was extracted at a pressure of 400 bar at a temperature of 70 캜 using a hot water bath. The mixture was concentrated to prepare a mixed extract.

The mixed extracts were fermented by rice bran, and then subjected to tertiary filtration to produce a fermentation broth.

[Experimental Example 1] Effect of improving sulfation

The following experiments were carried out to investigate the effect of fermentation of rice bran fermentation broth on the improvement of sulfation.

The cosmetics containing 5% by weight of the fermentation liquid of Example 1 and Comparative Examples 1 to 3 were exposed to water park personnel 12 persons (3 persons in each case) for 6 months from May to October, I was right on my back and my foot once a day.

As a result, the skin cell concentration was lowered as shown in Table 1 below, indicating that the sulfation improvement effect was exhibited.

Skin concentration (average value), pH concentration Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 existing 7.7 6.9 7.1 7. 4 6 months later 4.9 6.1 6. 2 5.7

[Experimental Example 2] Whitening improvement effect

The following experiment was conducted to investigate the effect of improving the whitening of fermented rice bran fermentation broth.

The cosmetics containing 5% by weight of the fermentation broth of Example 1 and Comparative Examples 1 to 3 were divided into 12 persons (3 persons for each case) who were normally dark skin, Respectively.

One year later, the color was measured in comparison with the existing skin. As a result, the whitening improvement effect was shown in Table 2 below.

Skin Brightness, Color Contrast Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 existing mum mum mum mum 1 year later Brown Brown Brown Brown

[Experimental Example 3] Skin Trouble Improvement Effect

The following experiment was carried out to investigate the effect of improving the skin troubles of fermented lactic acid bacteria fermented with rice bran.

Cosmetics containing 5% by weight of the fermentation broth of Example 1 and Comparative Examples 1 to 3 were applied to the face and back for 12 times (three persons for each case) with a large number of acne, once a day for one year.

As a result of measuring the distribution of acne after one year, it was found that the skin troubles were improved as shown in Table 3 below.

The acne index (the average number of acne spreads on the back and face) Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 existing 86 71 126 78 1 year later 24 55 93 52

[Experimental Example 4] Wrinkle improving effect

The following experiment was carried out to investigate the effect of improving the wrinkles of lactic acid fermentation broth fermented with rice bran.

Cosmetics containing 5% by weight of the fermentation broth of Example 1 and Comparative Examples 1 to 3 were applied to 12 persons (40 persons in each age group, 50 persons in each case) for one month for 6 months from May to October I did it right.

As a result of measuring the degree of wrinkling after one year in the eyes, it can be seen that the wrinkle-improving effect is shown in Table 4 below.

Wrinkle index (in snow) Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 existing Severe Universal Universal Severe 1 year later A noticeable improvement Improving Improving Universal

As described above, the cosmetic prepared from the fermented broth of lactic acid bacteria containing other fermented lactic acid bacteria and the fermented broth containing no lactic acid bacteria showed some improvement in sulfation, whitening, skin troubles and wrinkles. However, It can be confirmed that the cosmetic prepared from the lactic acid fermentation broth containing lactose exhibits a remarkable effect.

While the present invention has been described in connection with what is presently considered to be practical exemplary embodiments, it is to be understood that the invention is not limited to the disclosed embodiments, but, on the contrary, You will understand. The embodiments described above are therefore to be considered in all respects as illustrative and not restrictive.

Claims (11)

Rice bran extract, soybean extract, golden extract, green tea extract, ladybug extract, Aloe vera extract, Aloe vera extract, Hwanggi extract, Kangyo extract, Chrysanthemum extract, Ogphi extract, Centella asiatica extract, A cosmetic composition comprising a lactic acid fermentation broth comprising a fermentation broth of a lactic acid bacterium obtained by fermenting a mixed extract containing an extract, a caspase extract, a flower peppermint leaf extract, a ginger extract, and an oat extract with a lactic acid bacterium.
The method according to claim 1,
The lactic acid bacteria fermentation broth may contain,
Wherein the fermented milk is fermented by a fermentation process.
The method according to claim 1,
The lactic acid bacteria fermentation broth may contain,
Wherein the cosmetic composition is selected from the group consisting of an essential cosmetic preparation, a color cosmetic composition, a hair cosmetic composition, a detergent composition, a shampoo composition, and a body cosmetic composition.
The method according to claim 1,
Wherein the lactic acid fermentation broth contains 0.01 to 10% by weight based on the total weight of the cosmetic composition.
The method according to claim 1,
The above-
Wherein the extract is extracted at a temperature of 40 to 80 캜 at a pressure of 300 to 500 bar by using a hot water bath.
The method according to claim 1,
The lactic acid bacteria,
A cosmetic composition comprising a lactic acid fermentation broth characterized by being Lactobacillus sp.
a) Rice bran, bean, golden, green tea, ladybug, lily of the valley, Aroebera, Hwanggi, allium, persimmon, Obtaining by precipitation;
b) mixing and pulverizing 1 to 2 parts by weight of the plants obtained in step a);
c) extracting the mixture of step b) with a boiler at a temperature of 40 to 80 DEG C at a pressure of 300 to 500 bar;
d) concentrating the extract extracted in step c) to obtain a mixed extract;
e) treating the mixed extract of step d) with lactic acid bacteria to obtain a lactic acid fermentation broth.
8. The method of claim 7,
The lactic acid bacteria of step e)
Wherein the lactic acid bacteria is Lactobacillus sp.
8. The method of claim 7,
The lactic acid bacteria of step e)
A method for producing a lactic acid fermentation broth comprising the steps of: inoculating an MRS medium, culturing in a shaking incubator, and subculturing.
8. The method of claim 7,
The lactic acid bacteria of step e)
Wherein the initial number of bacteria is adjusted to 107 ~ 109 CFU / ml.
8. The method of claim 7,
The lactic acid fermentation broth of step e)
Wherein the fermentation broth is subjected to primary or tertiary filtration.

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