KR20150056314A - Composition for promoting collagen synthesis - Google Patents

Abstract

The present invention relates to a collagen synthesis accelerating composition including the extract of one or more plant varieties selected from a group including Fraxinus rhynchophylla, Marrubium vulgare, and Starchys officinalis or a fermented fraction thereof. The present invention relates to a method for preventing, alleviating, or treating skin wrinkles using the collagen synthesis accelerating composition. The present invention also relates to a cosmetic composition, a food composition, a quasi-composition, and pharmaceutical composition containing the collagen synthesis accelerating composition.

Description

[Composition for promoting collagen synthesis]

The present invention relates to a composition for accelerating collagen synthesis, which comprises a fermented product of an extract of at least one plant selected from the group consisting of Aspergillus oryzae, bitter pepper, and fenugreek mulberry or a fraction thereof. The present invention also relates to a method for preventing, improving or treating skin wrinkles using the composition for promoting collagen synthesis. The present invention also relates to a cosmetic composition, a food composition, a quasi-drug composition and a pharmaceutical composition containing the composition for promoting collagen synthesis.

Collagen, a major component of the extracellular matrix, is a major substrate protein produced by the fibroblasts of the skin. In addition, it is an important protein occupying about 30% of the total weight of the bioprotein, and has a solid triple helix structure. Collagen forms most of the organic matter in the skin, tendons, bones and teeth, especially in bone and skin (jaw). In most other body structures, it exists as a fibrous inclusion.

Collagen is a relatively weak immunogen, partly due to the blocking of the potential antigenic determinant by the helical structure of the collagen, which also causes the collagen to be resistant to proteolysis. The major functions of collagen are known to be mechanical rigidity of skin, resistance of connective tissues and binding force of tissues, support of cell adhesion, induction of cell division and differentiation (when organism grows or heals the wound) (Van der Rest et al. Ann NY Acad Sci, 1990).

Such collagen is reduced by aging and photoaging by ultraviolet irradiation, which is known to be closely related to the wrinkling of the skin (Arthur K. Balin et al., Aging and the skin, 1989). Collagen also plays an important role in wound healing and can accelerate the synthesis of collagen in the damaged epithelium, allowing quick, scarless wound healing.

Conventionally, products using collagen in a composition for external application for skin such as cosmetics or ointment have been marketed in order to utilize the skin moisturizing effect and wound healing effect of collagen. However, these products apply collagen itself to the skin surface, It is impossible to expect a moisturizing action or a wound healing effect, so that it can not be said that it exhibits essential skin function improvement and wound healing function.

In order to solve this problem, there has been a growing interest in collagen synthesis promoting substances. Vitamin C, retinoic acid, transforming growth factor (TGF) (Cardinale G. et al. (Japanese Patent Application Laid-open No. Hei 8-231370), betulinic acid (Japanese Patent Application Laid-Open No. 8-208424) Chlorella extract (JP-A-9-40526, JP-A-10-36283, fibroblast proliferation promoting action), and the like.

However, these materials have limited use due to safety problems such as irritation and reddening when applied to the skin, or the effect is insignificant and virtually no effect of skin function improvement or wound healing can be expected. Therefore, there is a desperate need to develop a novel composition for external skin application that is safer and more effective in living body than existing skin external composition compositions.

On the other hand, fermentation is a process of decomposing organic matter using enzymes possessed by microorganisms. Fermentation and corruption proceed by a similar process, but decomposition is called fermentation when substances that are useful for our lives are made, and when they make odorous or harmful substances, it is called corruption. Therefore, when the medicinal plant is fermented using microorganisms, the toxicity of the medicinal plant is reduced, the medicinal plant is reduced in molecular weight to facilitate digestion, and the transdermal absorption upon application to the skin is facilitated and the bioavailability is improved.

In addition, medicinal plants themselves can exhibit pharmacological action, but the pharmacological effect can be further enhanced through fermentation, thereby exhibiting a new pharmacological effect which is not present in the medicinal plant before fermentation.

Under these circumstances, the inventors of the present invention conducted studies on substances having excellent collagen synthesis promoting effect, skin wrinkle improving effect, wound healing effect and persistence of effect thereof, and thus, It has been confirmed that the fermented product of at least one selected plant extract or fraction thereof has an effect of promoting collagen synthesis, and the composition for accelerating collagen synthesis utilizing the same is completed.

The present invention aims at promoting collagen synthesis by using a composition containing as an active ingredient a fermented product of at least one plant extract or a fraction thereof of a plant selected from the group consisting of Aspergillus oryzae, bitter pepper, and fenugreek.

Specifically, it is an object of the present invention to provide a cosmetic, a food, a quasi-drug or a pharmaceutical composition for preventing, improving or treating skin wrinkles using the composition for promoting collagen synthesis.

It is still another object of the present invention to provide a method for preventing, improving or treating wrinkles of skin using the composition for promoting collagen synthesis.

In order to solve the above problems, there is provided, as one embodiment of the present invention, a method for promoting collagen synthesis comprising an extract of at least one plant selected from the group consisting of asparagus, bitter pepper, ≪ / RTI >

In another embodiment of the present invention, the composition for promoting collagen synthesis may further contain a fermentation product of at least one plant extract or a fraction thereof selected from the group consisting of black cumin, have.

The Fraxinus of the present invention Excelsior ) is a deciduous broad-leaved arboreous tree belonging to the Fraxinus, which is native to Europe and distributed in mountains of Korea. It is used for antioxidant, antiviral and antimicrobial effects. Caffeic acid, which is isolated from the leaves, is known as a compound isolated from the gooseberry.

Marrubium Vulgare of the present invention is a perennial herb belonging to Lamiaceae and has anti-inflammatory and antihistaminic effects. In addition, bitter peppermint stimulates the secretion of gastric fluid and bile, and acts as a goddess. Therefore, it can be used for digestive disorders such as abdominal bloating and gas, and can be used for relieving inflammation of bronchial tubes. Compounds isolated from bitter peppermint are known to be anionic and tannin.

Stachys officinalis of the present invention is a perennial herb belonging to Lamiaceae, originating in Europe and distributed in western Asia, North America, and the like. It is good for arthritis and gout, and it is effective for relieving hangovers.

Nigella sativa of the present invention is an annual herb that belongs to Ranunculaceae and is used for immunity enhancement, allergy and chemotherapy. Compounds isolated from the bulk cocaine include thymoquinone, thymohydroquinone, dithymoquinone, thymol, carvacrol, and the like. It is also known that the extracts and active ingredients of the extracts and their active ingredients have a pharmacological effect including anti-inflammatory, anti-hyperglycemic, anti-hypertensive, anti-asthmatic, antibacterial, antiparasitic, antioxidant and anti-cancer effects.

Valeriana Officinalis is a perennial herb belonging to Valerianaceae and originates in Europe and northern Asia, and is distributed in Korea, Japan, Sakhalin, Taiwan, and northeastern China. In Europe, it has been used for diuretics, painkillers, bruises and the like, and now it is used for hysteria and neurosis. In addition, the roots of Oriental herbaceous plants are used as medicines for mental anxiety, nervous breakdown, myocarditis, and stomach cramps.

Rheum Palmatum of the present invention is a perennial upright herb belonging to Polygonaceae and has antibacterial and hemostatic action. In addition, pharmacological actions of Jangbyeol Rhubarb are known to promote colonic motility, secretory action, antipyretic effect, body temperature drop, promotion of bile secretion, shortening of blood coagulation time, antibacterial action, diuretic and liver function protection. Emodin is known as a compound isolated from Changdyeon rhubarb.

The term "extract" used in the present invention refers to an extract obtained by an extraction treatment of each plant, a diluted solution or a concentrate of the extract, a dried product obtained by drying the extract, a controlled preparation or a purified product of the extract, Etc., the extract itself and extracts of all the formulations which can be formed using the extract. The extract or fraction of the present invention can be prepared and used in the form of a dry powder after extraction.

The extract of the present invention can be extracted from various organs of natural, hybrid, or variant plants of each of the above plants. For example, the extracts of roots, upper parts, stems, leaves, flowers, It can be extracted not only from shells, seeds, but also from plant tissue cultures.

The extract of Aspergillus oryzae according to the present invention may be an extract of leaves of an aspergillus oryzae.

The bitter pepper extract of the present invention may preferably be an extract of the ground part of bitter peppermint.

The extract of the present invention may be an extract of the above-ground part of the ferns.

The black cumin extract of the present invention may preferably be an extract of the above ground part of blackcumin.

The Agaricus vulgaris extract of the present invention may preferably be an extract of the above-ground part of the Agaricus vulgaris.

The long-leaf rhubarb extract of the present invention may be an extract of the above-ground rhubarb root.

In each of the above-mentioned plant extracts of the present invention, the method for extracting the respective plants is not particularly limited, and extraction can be carried out according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration, and reflux extraction. These may be performed alone or in combination with two or more methods.

In the present invention, the kind of the extraction solvent used for extracting the respective plants is not particularly limited, and any solvent known in the art can be used. Nonlimiting examples of the extraction solvent include water, alcohols or mixed solvents thereof. When alcohol is used as a solvent, C ₁ to C ₄ alcohols may be more preferably used. Each of the plant extracts of the present invention may preferably be water or a methanol extract.

More specifically, in one embodiment of the present invention, at least one plant selected from the group consisting of migratory as well as bitter mint, bitter mint, broad bean mallow, black cumin, , Heating in an extractor equipped with a reflux condenser at 50 to 100 ° C for 1 to 5 hours using 5 to 20 parts by volume of water or an organic solvent such as anhydrous C ₁ to C ₄ or lower alcohol at a dry weight of the pulverized material And the extract of each plant is extracted. After filtration with a filter cloth, the residue is further extracted one or more times in the same manner as described above, and the extracts are combined, concentrated under reduced pressure, and lyophilized or spray-dried to obtain a dried extract.

The term "fraction " as used herein means a product obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.

The fractionation method for obtaining the fraction in the present invention is not particularly limited and may be carried out according to a method commonly used in the art. As a non-limiting example of the above-described fractionation method, there can be mentioned a method in which a fraction obtained from the above extract is obtained by treating a predetermined solvent with an extract obtained by extracting each plant.

The kind of the fraction solvent used for obtaining the fraction in the present invention is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the fraction solvent include polar solvents such as water and alcohol; And non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more. When alcohols are used in the fraction solvent, C ₁ to C ₄ alcohols can be preferably used.

The term "fermentation" used in the present invention means that the microorganism is not a corruption reaction during the process of decomposing an organic substance by using an enzyme contained in the microorganism. The fermentation reaction and the corruption reaction proceed by a similar process, but when the useful substance is made from the decomposition, it is called fermentation. If it is made bad smell or harmful substance, it is called corruption.

The term "fermentation product" as used in the present invention means a useful substance produced through the fermentation process.

In another embodiment of the present invention, the fermented product is at least one selected from the group consisting of yeast fermented product, lactic acid fermented product and bifidobacterial fermented product of the extract of the plant or a fraction thereof.

The method for obtaining the fermentation product from the yeast, lactic acid bacterium and bifidobacteria in the present invention is not particularly limited, and a fermentation product can be obtained according to a method commonly used in the technical field or the like.

In the present invention, the method for obtaining a fermentation product from the yeast, lactic acid bacteria or bifidus bacteria is characterized in that the extract of each of the above plants is added to a basic medium for yeast, lactic acid bacteria or bifidus bacteria and each corresponding fermentation strain, yeast, Treating the bifidobacteria and fermenting them. The temperature, fermentation time, etc. of the fermentation process are not particularly limited and may be variously selected depending on fermentation precursor materials and the like.

In the present invention, the plant extract or fraction thereof may be added to the medium for culturing yeast, lactic acid bacteria or bifidobacteria, preferably 1 to 20 wt%, more preferably 1 to 10 wt% By weight, and more preferably 3% by weight to 7% by weight. There is an advantage that a fermentation product having excellent collagen synthesis promoting ability can be obtained within the above range.

In a further embodiment of the invention, the yeast is in my process to the three Levy Jia Saccharomyces (Saccharomyces cerevisiae), ski irradiation Caro My process ellipsometer Id (Schizosaccharomyces ellipsoids) and be at least one selected from the group consisting of saccharose as MY bowl radio access (Saccharomyces boulardii) and, without being limited thereto.

In another embodiment of the invention, the lactic acid bacterium is selected from the group consisting of Lactobacillus < RTI ID = 0.0 > acidophilus , Lactobacillus Casei (Lactobacillus casei ), Lactobacillus Bulgaria kusu (Lactobacillus bulgaricus), Lactobacillus Caucasus ramno (Lactobacillus rhamnosus ), Lactobacillus Planta Room (Lactobacillus plantarum), ruteri Lactobacillus bacteria (Lactobacillus reuteri), Lactobacillus konpu Seuss (Lactobacillus confusus ), Lactobacillus Fur momentum (Lactobacillus fermentum) and Lactobacillus brevis (may be at least one selected from the group consisting of Lactobacillus brevis), but is not limited thereto.

In another embodiment of the present invention, the Bifidobacteria is Bifidobacterium breve (Bifidobacterium breve ), Bifidobacterium BP Doom (Bifidobacterium bifidum ), bifidobacterium Ronggeom (Bifidobacterium longum) and Bifidobacterium Infante tooth (may be more than one type of compound selected from the group consisting of Bifidobacterium infantis), but is not limited thereto.

Specifically, when a yeast is used as a microorganism, it is preferable to use a basic medium containing an extract of one or more plants selected from the group consisting of black cumin, red pepper, bitter pepper, YM medium), preferably about 10 < ⁵ > to 10 < ⁷ > cells / mL and incubated for 30 to 120 hours at a pH of ^5.5 to 6.5, a temperature of 25 to 35 DEG C, Lt; / RTI > Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the yeast body, and the filtrate is concentrated under reduced pressure in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, whereby each fermentation product can be obtained.

On the other hand, when a lactic acid bacterium is used as a microorganism, a basic medium (MRS) containing an extract of one or more plants selected from the group consisting of black cumin, red pepper, bitter pepper, Preferably 10 ⁵ to 10 ⁷ cells / mL, and cultured for 24 to 120 hours under the conditions of pH 6.5 to 7.5, temperature 35 to 38 ° C, aeration amount 1 VVM. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the lactic acid bacterium, and the filtrate is concentrated under reduced pressure in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, whereby each fermentation product can be obtained.

When bifidobacteria are used as the microorganisms, the extracts or fractions thereof of the plant selected from the group consisting of black cumin, red pepper, bitter pepper, BL medium) is inoculated with bifidobacteria preferably at about 10 ⁵ to 10 ⁷ cells / mL, followed by culturing at pH 6.5 to 7.5, at a temperature of 35 to 38 ° C for 24 to 120 hours while maintaining anaerobic culture conditions. Thereafter, the fermentation broth is centrifuged to remove the polymer precipitate and the bifidus microbial cells, followed by concentration under reduced pressure in a distillation apparatus equipped with a cooling condenser, followed by lyophilization, whereby each fermentation product can be obtained.

In the present invention, the fermented product obtained from each of the fermentation strains is not only the fermented substance itself but also a fermentation strain culture medium in which a fermentation strain and a fermentation product coexist, a fermentation product obtained by centrifuging the fermentation strain from the culture medium, A fermentation product obtained by filtering a fermentation strain from a culture medium, a fermentation product sterilized and filtered from the culture medium, an extract obtained by extracting the fermentation product or a culture medium containing the same, a fermentation product obtained by diluting the fermentation product or its extract , A fermented product obtained by drying the fermented product or an extract thereof, and a fermentation product derived from the fermentation product.

In the present invention, the method for obtaining the above fermented product can be preferably obtained by centrifuging and removing the solid material including the fermentation strain after completion of fermentation.

In addition, the fermented product of the present invention can be produced in a powder state by an additional process such as concentration under reduced pressure and lyophilization or spray drying.

In the present invention, the above-mentioned fermented product is preferably a yeast fermentation product of an extract or fraction thereof of at least one plant selected from the group consisting of Siberian lettuce, bitter pepper, Prussian japonica, black cumin, A fermented product and a fermented product of bifidobacteria. Specifically, the mixed fermented product may be an extract of at least one plant selected from the group consisting of Siberian lettuce, bitter pepper, Prussian japonica, black cumin, Fermented lactic acid bacteria fermented with lactic acid bacteria, and fermented bifidobacteria fermented with bifidobacteria may be mixed together. In the case of the fermented yeast fermented product, lactic acid fermented product and bifidobacterial fermented product, there is an advantage of further promoting collagen synthesis promotion effect.

In the composition for accelerating collagen synthesis according to the present invention, the total amount of the fermentation product may be 0.0001 to 20% by weight, more preferably 0.01 to 20% by weight, based on the total weight of the composition for promoting collagen synthesis, % To 10% by weight. Within the above range, there is an advantage that the collagen synthesis promoting ability is excellent without cytotoxicity. Particularly, when it is less than 0.0001% by weight, a remarkable effect can not be expected.

The composition for promoting collagen synthesis containing the fermented product of the extract of one or more kinds of plants selected from the group consisting of Aspergillus oryzae, bitter pepper and fenugreek extract of the present invention or a fraction thereof as an active ingredient has been confirmed to have collagen synthesis promoting ability , Which was first identified in the present invention. Further, since the composition of the present invention is a substance derived from a natural material, it is not toxic, and even if it is applied to human body, it does not cause side effects and can be utilized as various cosmetic composition, food composition, pharmaceutical composition and quasi-drug composition.

As used herein, the term "collagen" is a protein which occupies the largest quantitative and functionally in the human body. It is an extracellular matrix that forms the skeleton of all cells in the human body, more specifically connective tissue). Human tissues containing large amounts of collagen include bone tissues such as skin, bones and cartilage, tendons, and ligaments. It is known that aging of the tissues causes human aging diseases such as skin wrinkles and sagging and osteoporosis, which is caused by a decrease in collagen amount and function due to decrease of collagen synthesis of tissues.

On the other hand, the main function of dermal fibroblasts is extracellular matrix synthesis and regeneration of injured skin tissue through proliferation. The extrinsic aging factors such as photoaging, accumulation of damage due to free oxygen, and endogenous Aging factors reduce the physiological activity of dermal fibroblasts in the dermal layer. Type I collagen, which accounts for more than 95% of the extracellular matrix and plays a major role in the physiological activity of fibroblasts through interactions and signal interactions with adhesion molecules and cytoskeletons of cells, Is reduced, the amount of collagen in the skin tissue is reduced, thereby decreasing the surface tension to form a skin wrinkle, and causing a vicious circle in which the physiological activity including cell proliferation and regeneration function is reduced do. Therefore, collagen of dermal fibroblasts may be directly related to restoration or regeneration of tissue in skin aging, skin wrinkle formation and skin damage.

That is, when the synthesis of collagen or procollagen of dermal fibroblast is promoted, it can prevent and improve wrinkles of skin, heal wounds, repair and regenerate damaged skin tissue, and prevent and improve skin aging.

Specifically, in an experimental example of the present invention, in the case of using the black cumin, even the mushroom, and the mushroom of the mushroom, and the mushroom of the mushroom, including bitter peppermint and bitter mushroom, respectively, In the case of no significant purification, it was confirmed that the fibroblasts had a very strong collagen synthesis promoting ability equal to or higher than that of Vitamin C, which is a known promoter of collagen synthesis (Experimental Example 1). Therefore, it can be seen that the composition for promoting collagen synthesis of the present invention comprising the fermented product of each of the plant extracts as an active ingredient has a strong collagen synthesis promoting ability, and thus can be very useful as a skin wrinkle remover or a wound healing agent .

According to another embodiment of the present invention, there is provided a method for producing a composition for promoting collagen synthesis comprising as an active ingredient a fermented product of an extract of at least one plant selected from the group consisting of Aspergillus oryzae, bitter pepper, .

Specifically, the method comprises: extracting at least one plant selected from the group consisting of migratory aspen, bitter pepper, and prickly clover; And fermenting the extract obtained in the extracting step with at least one strain selected from the group consisting of yeast, lactic acid bacteria and bifidobacteria.

In another embodiment of the present invention, the fermentation step comprises fermenting the extract with a yeast, a lactic acid bacterium or a bifidus bacterium, respectively, to obtain a yeast fermented product, a lactic acid fermented product and a bifidobacterial fermented product; And mixing the yeast fermented product, the lactic acid bacterium fermented product and the bifidobacterial fermented product to produce a mixed fermented product.

In the above production method of the present invention, the description of each of the above steps is the same as described above in connection with the composition of the present invention.

As another embodiment of the present invention, there is provided a cosmetic composition for preventing or improving skin wrinkles containing the composition for promoting collagen synthesis as an active ingredient.

The term "prevention" as used in the present invention means any action that suppresses or delays the occurrence of skin wrinkles by administering the composition of the present invention to an individual.

As used herein, the term "improvement" means any action that at least reduces the degree of symptom associated with the condition being treated.

The cosmetic composition according to the present invention can be used as a cosmetic composition in the form of a solution, an external ointment, a cream, a foam, a nutritional lotion, a soft lotion, a perfume, a pack, a soft water, The composition may be formulated as a suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, But is not limited thereto.

The cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier to be incorporated in a cosmetic composition for general skin. Examples of the cosmetic composition include ordinary ingredients such as oil, water, a surfactant, a moisturizer, a lower alcohol, , A chelating agent, a coloring agent, a preservative, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier to be contained in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulations of the present invention are ointments, pastes, creams or gels, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicon, bentonite, silica, talc, zinc oxide May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder and the like may be used as a carrier component. In particular, But are not limited to, propellants such as rocaborn, propane / butane or dimethyl ether. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent may be used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like can be used, and particularly fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a soap, use is made of an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolizate, isethionate, a lanolin derivative, an aliphatic alcohol, a vegetable oil, glycerol, But is not limited thereto. These may be used alone or in combination of two or more.

As another embodiment of the present invention, there is provided a food composition for preventing or improving skin wrinkles containing the composition for promoting collagen synthesis as an active ingredient.

The food composition of the present invention is not particularly limited and includes a health functional food composition.

When the health functional food composition of the present invention is used as a food additive, the composition may be added as it is or may be used together with other food or food ingredients, and may be appropriately used according to a conventional method.

The kind of the food is not particularly limited, and includes food in a conventional sense. Non-limiting examples of the food to which the substance can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice creams, , A drink, an alcoholic beverage, and a vitamin complex.

When the health functional food composition of the present invention is a beverage composition, various flavoring agents, natural carbohydrates, and the like may be contained as additional components such as ordinary beverages. Non-limiting examples of such natural carbohydrates include monosaccharides such as glucose and fructose; Disaccharides such as maltose and sucrose; Natural sweetening agents such as dextrin, cyclodextrin; Synthetic sweetening agents such as saccharin and aspartame, and the like. The proportion of the additional component added may be appropriately determined by a person skilled in the art.

In addition to the above, the health functional food composition of the present invention may contain various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloid thickening agents, pH adjusting agents, stabilizers, , Alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the health functional food composition of the present invention may contain pulp for the production of natural fruit juice, fruit drink or vegetable drink. These components may be used independently or in combination of two or more. The ratios of these additives can also be suitably selected by those skilled in the art.

As another embodiment of the present invention, there is provided a quasi-drug composition for preventing or improving skin wrinkles containing the composition for promoting collagen synthesis as an active ingredient.

The quasi-drug composition of the present invention can be used as a body cleanser, a disinfectant cleaner, a detergent, a kitchen detergent, a cleaning detergent, a toothpaste, a gauze agent, a wet tissue, a detergent, a soap, a hand wash, a hair cleanser, a hair softener, a humidifier filler, But are not limited thereto.

According to still another embodiment of the present invention, there is provided a pharmaceutical composition for preventing or treating skin aging comprising the composition for promoting collagen synthesis as an active ingredient.

In another embodiment of the present invention, the prevention or treatment of skin aging is performed by preventing or improving wrinkles of the skin.

The term "treatment" as used in the present invention means all the actions to improve or improve symptoms of skin wrinkles by administering the composition of the present invention to an individual.

The pharmaceutical composition of the present invention may further comprise a pharmaceutically acceptable carrier in addition to the above fermented product as an active ingredient.

In the present invention, the above-mentioned "pharmaceutically acceptable" means that it is commonly used in the pharmaceutical field, which does not disturb the biological activity and properties of the compound administered, without irritating the organism upon administration thereof.

The pharmaceutical composition of the present invention may be formulated together with the carrier to be used as medicines, feed additives, drinking water additives, and the like.

In the present invention, the type of the carrier is not particularly limited and any carrier conventionally used in the art can be used. Examples of the carrier include, but are not limited to, saline, sterilized water, Ringer's solution, buffered saline, albumin injection solution, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, maltodextrin, glycerol, . These may be used alone or in combination of two or more.

In addition, the pharmaceutical composition of the present invention may be supplemented with other pharmaceutically acceptable additives such as excipients, diluents, antioxidants, buffers or bacteriostats, and may be used as fillers, extenders, wetting agents, disintegrants, , A binder, a lubricant, and the like may be additionally used.

The pharmaceutical composition of the present invention may be formulated into various formulations suitable for oral administration or parenteral administration.

Non-limiting examples of such oral dosage forms include troches, lozenges, tablets, aqueous suspensions, oily suspensions, prepared powders, granules, emulsions, hard capsules, soft capsules, syrups or elixirs .

In order to formulate the pharmaceutical composition of the present invention for oral administration, a binder such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, cellulose, or gelatin; Excipients such as dicalcium phosphate and the like; Disintegrating agents such as corn starch or sweet potato starch; Lubricants such as magnesium stearate, calcium stearate, sodium stearyl fumarate or polyethylene glycol wax may be used, and sweeteners, fragrances, syrups and the like may also be used. .

Furthermore, in the case of capsules, in addition to the above-mentioned substances, liquid carriers such as fatty oils can be further used.

Non-limiting examples of the parenteral preparation include injections, suppositories, respiratory inhalation powders, aerosol preparations for spraying, ointments, powder for application, oils, creams and the like.

In order to formulate the pharmaceutical composition of the present invention for parenteral administration, sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried preparations and external preparations may be used. Examples of the non-aqueous solutions and suspensions include propylene glycol, polyethylene Glycerol, vegetable oils such as olive oil, injectable esters such as ethyl oleate, and the like.

More specifically, when the pharmaceutical composition of the present invention is formulated into an injection, the composition of the present invention is mixed with water or a stabilizer or buffer in water to prepare a solution or suspension, which is then mixed with an ampoule or a vial Unit dosage form. In addition, when the pharmaceutical composition of the present invention is formulated into an aerosol formulation, a propellant or the like may be added together with the additive such that the water-dispersed concentrate or the wet powder is dispersed.

When the pharmaceutical composition of the present invention is formulated into an ointment, cream, or the like, it is possible to use an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, a polyethylene glycol, a silicone, a bentonite, Or the like can be used as a carrier.

The pharmaceutically effective amount and the effective dose of the pharmaceutical composition of the present invention may be varied depending on the formulation method, administration method, administration time and / or route of administration of the pharmaceutical composition, Including, but not limited to, the type and severity of the disease, the type of subject being treated, the age, body weight, general health, severity or severity of the disease, sex, diet, excretion, Can be varied according to various factors and similar factors well known in the medical field, and those skilled in the art can easily determine and prescribe effective dosages for the desired treatment.

The dosage for the more preferable effect of the pharmaceutical composition of the present invention may be preferably 1 mg / kg to 1,000 mg / kg per day, more preferably 10 mg / kg to 500 mg / kg per day. The pharmaceutical composition of the present invention may be administered once a day or divided into several doses. Accordingly, the dosage is not limited in any way to the scope of the present invention.

The route of administration and the mode of administration of the pharmaceutical composition of the present invention may be independent of each other, and the method is not particularly limited, and any route of administration and administration method may be used as long as the pharmaceutical composition can reach the desired site You can follow. The pharmaceutical composition may be administered orally or parenterally.

The parenteral administration may be, for example, intravenous administration, intraperitoneal administration, intramuscular administration, transdermal administration or subcutaneous administration, and a method of applying, spraying or inhalation the composition to a diseased site may also be used But are not limited to.

The pharmaceutical composition of the present invention can preferably be administered orally or by injection.

As another embodiment of the present invention, there is provided a method for preventing or improving skin wrinkles comprising the step of administering the composition for promoting collagen synthesis to a subject.

The term "individual" as used herein refers to all animals, including mammals including rats, livestock, humans, and the like.

In the skin wrinkle preventing or improving method of the present invention, the dose, route of administration, administration mode, etc. of the collagen synthesis promoting composition are the same as those described above in connection with the pharmaceutical composition of the present invention.

In another embodiment of the present invention, there is provided a method for preventing or treating skin aging comprising the step of administering the pharmaceutical composition to an individual other than a human.

As used herein, the term "an individual excluding a human being" means all animals including mammals including rats, livestock and the like except humans.

In the method for preventing or treating skin aging according to the present invention, the dose, route of administration, mode of administration, etc. of the pharmaceutical composition are the same as those described above in connection with the pharmaceutical composition of the present invention.

The composition for accelerating collagen synthesis according to the present invention enhances the pharmacological effect of each plant through fermentation while safely protecting the skin, thereby effectively promoting collagen synthesis, thereby improving the wrinkle of the skin, the wound healing effect and the persistence of the effect.

Hereinafter, the present invention will be described in more detail by way of examples. It should be understood, however, that these examples are for illustrative purposes only and are not to be construed as limiting the scope of the invention.

Production Example 1: Preparation of plant extract

200 g of each ground product obtained by purchasing commercially available black cumin, red peppermint, bitter peppermint, primrose liquorice, persimmon grass and long-leaf lemon juice were put into purified water or 3 L of 80% methanol, It was boiled and extracted for 3 hours. After filtration through a 100-mesh filter cloth, the residue was extracted one more time by the same method. The extracts were combined and filtered through a filter paper of Whatman No. 2 at room temperature to remove insoluble materials. The extracts were concentrated under reduced pressure at 60 ° C in a distillation apparatus equipped with a condenser, and then lyophilized to obtain the respective dry weights ≪ / RTI > Table 1 below shows the dry weight of the extract per 100 g of each plant according to the extraction solvent.

plant 80% methanol Purified water Black cumin 8.0 g 7.5 g Gourd ashore 8.5 g 7.7 g Bitter mint 7.9 g 7.5 g About 8.4 g 7.6 g Even if you have a gullible pool 8.2 g 7.6 g Changdeok Rhyu 8.2 g 7.5 g

Example 1: Yeast fermentation of plant extracts

Each of the plant extracts prepared in Preparation Example 1 was added to YM medium as a basic medium for saccharomyces cerevisiae, which was a yeast strain, in an amount of 5% by weight and fermented.

Specifically, my process as Saccharomyces Was inoculated in three Levy Jia about 10 ⁵ ~10 ⁷ cells / mL yeast basal medium (YM medium) is fermented at 5% by weight of a concentration of the to, pH 5.5~6.5, temperature 25~35 ℃, barrel And cultured for 48 hours while maintaining the condition of 0.05-0.5 VVM. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the yeast strain, and the suspension was concentrated under reduced pressure at 60 DEG C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization to finally obtain a yeast fermentation product of each plant . The dry weight of the yeast fermentation product of each plant is shown in Table 2 below.

plant Dry weight (g) of yeast fermentation Black cumin 5.11 g Gourd ashore 5.42 g Bitter mint 5.38 g About 5.34 g Even if you have a gullible pool 5.22 g Changdeok Rhyu 5.19 g

Example 2: Production of fermented lactic acid bacteria from plant extracts

Each of the plant extracts prepared in Preparation Example 1 was dissolved in lactobacillus Was added to the MRS medium as the basic medium for the Acidophilus at 5% by weight and fermented.

Specifically, Lactobacillus Also know after inoculation the filler's at about 10 ⁵ ~10 ⁷ cells / mL of Lactobacillus basal medium (MRS medium) containing a concentration of each plant extract of Preparation Example 1 in 5 wt.%, PH 6.5~7.5, And cultured for 48 hours at a temperature of 35 to 38 DEG C while maintaining a ventilation amount of 1 VVM. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and the lactic acid bacteria, followed by concentration under reduced pressure at 60 DEG C in a distilling apparatus equipped with a cooling condenser, followed by lyophilization, finally obtaining a lactic acid fermentation product of each plant . The dry weight of the lactic acid fermented product of each plant is shown in Table 3 below.

plant Dry weight (g) of lactic acid fermentation Black cumin 5.20 g Gourd ashore 5.39 g Bitter mint 5.29 g About 5.46 g Even if you have a gullible pool 5.47 g Changdeok Rhyu 5.42 g

Example 3: Preparation of bifidobacterial fermentation product of plant extract

Each of the plant extracts prepared in Preparation Example 1 was added to BL medium as a basic medium for Bifidobacterium bifidobacterium long sword at 5 wt% and fermented.

Specifically, Bifidobacterium longidum was inoculated into a bifidobacterial basic medium (BL medium) containing 5% by weight of each plant extract of Preparation Example 1 at a concentration of about 10 ⁵ to 10 ⁷ cells / mL, pH 6.5-7.5, temperature 35-38 ° C, and anaerobic culture conditions were maintained for 48 hours. Thereafter, 100 g of each fermentation broth was centrifuged to remove the polymer precipitate and bifidus bacterium, followed by concentration under reduced pressure at 60 DEG C in a distillation apparatus equipped with a cooling condenser, followed by lyophilization to finally obtain the bifidobacterial fermentation product of each plant Respectively. The dry weight of each bifidus fermented product is shown in Table 4 below.

plant Dry weight (g) of bifidobacterial fermentation Black cumin 5.33 g Gourd ashore 5.45 g Bitter mint 5.27 g About 5.44 g Even if you have a gullible pool 5.51 g Changdeok Rhyu 5.54 g

Experimental Example 1: Promotion of collagen synthesis promoting effect of fermented product of plant extracts

Each of the black cumin, aspartic acid, bitter mint, broad-leaved mung bean, Safflower, or long-stemmed rhubarb yeast, lactic acid bacterium or bifidobacterium fermentation product prepared in Examples 1 to 3 was added to a culture solution of human-derived fibroblasts, Collagen synthesis effect was examined. The synthesized collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immunoassay KIT).

Specifically, the fermented products of black cumin, red pepper, bitter pepper, fenugreek mugwort, pseudomonas sp. Or rhododendron rhizoma prepared in Examples 1 to 3 were dissolved in a final concentration of 0.0005% (w / w) After culturing for one day, the culture broth was taken and the degree of collagen synthesis was measured at 450 ㎚ using a PICP EIA Kit using a spectrophotometer at each concentration. For the comparison of the effects, the samples were added to the culture medium (control group) of the fibroblasts to which nothing had been added (non-fermented extract) and the vitamin C to the final concentration of 0.0005% (w / w) Were measured.

The amount of collagen production was measured by UV absorbance, and the rate of increase of collagen production was calculated as a ratio of the amount of collagen production relative to the control. The results are summarized in Table 5 below.

sample Growth rate (%) Control group (no addition) - YM medium (final concentration 0.0005%) 105.12 MRS medium (final concentration 0.0005%) 104.5 BL medium (final concentration 0.0005%) 103.2 Black cumin extract 122.35 Yeast fermentation product (final concentration 0.0005%) 143.25 Fermented product of lactic acid bacteria (final concentration 0.0005%) 148.12 Bifidobacterial fermentation product (final concentration 0.0005%) 145.21 Gourd ashore extract 115.22 Yeast fermentation product (final concentration 0.0005%) 139.58 Fermented product of lactic acid bacteria (final concentration 0.0005%) 135.77 Bifidobacterial fermentation product (final concentration 0.0005%) 141.24 Bitter mint extract 115.27 Yeast fermentation product (final concentration 0.0005%) 142.76 Fermented product of lactic acid bacteria (final concentration 0.0005%) 141.31 Bifidobacterial fermentation product (final concentration 0.0005%) 144.22 About extract 119.14 Yeast fermentation product (final concentration 0.0005%) 143.91 Fermented product of lactic acid bacteria (final concentration 0.0005%) 148.18 Bifidobacterial fermentation product (final concentration 0.0005%) 143.55 Even if you have a gullible pool extract 120.30 Yeast fermentation product (final concentration 0.0005%) 141.48 Fermented product of lactic acid bacteria (final concentration 0.0005%) 140.93 Bifidobacterial fermentation product (final concentration 0.0005%) 141.98 Changdeok Rhyu extract 118.27 Yeast fermentation product (final concentration 0.0005%) 142.33 Fermented product of lactic acid bacteria (final concentration 0.0005%) 145.88 Bifidobacterial fermentation product (final concentration 0.0005%) 140.53 Vitamin C (final concentration 0.0005%) 134.76

As shown in Table 5 above, the fermented products of black cumin, goose ash, bitter pepper, fenugreek mugwort, fenugreek, or long-stemmed rhubarb prepared in Examples 1 to 3 of the present invention were prepared using the basic medium or non- fermented Considering the fact that the amount of collagen produced in human fibroblasts was significantly increased and that the cells were not purified at all, it was found that fibroblasts had the ability to promote very strong collagen synthesis more than or equal to that of vitamin C, which is a known promoter of collagen synthesis I could.

Therefore, the composition for promoting collagen synthesis of the present invention has a strong collagen synthesis promoting ability without exhibiting cytotoxicity at a concentration of 0.0005% (w / w) or higher, and thus can be very useful as a skin wrinkle remover or a wound healing agent Could know.

Example  4 to 9: of the plant extract Fermented  Preparation of mixture

The yeast, lactic acid bacteria or bifidobacterial fermented product and purified water of the black cumin, the aspartame, the bitter pepper, the fenugreek, the fenugreek or the fenugreek rhizoma prepared in Examples 1 to 3 were mixed as shown in Table 6 below being).

Example 4 Example 5 Example 6 Example 7 Example 8 Example 9 Black cumin leaven 20 Lactobacillus 20 Bifidus 20 Gourd ashore leaven 20 Lactobacillus 20 Bifidus 20 Bitter mint leaven 20 Lactobacillus 20 Bifidus 20 About leaven 20 Lactobacillus 20 Bifidus 20 Even if you have a gullible pool leaven 20 Lactobacillus 20 Bifidus 20 Changdeok Rhyu leaven 20 Lactobacillus 20 Bifidus 20 Purified water 40 40 40 40 40 40

Examples 10 to 15 and Comparative Example 1: Production of a cream using a fermentation mixture of the plant extract of the present invention

Creams were prepared according to the prescriptions of the following Table 7 using the fermentation mixtures of black cumin, goup, bitter pepper, ginger, and the like prepared in Examples 4 to 9.

Example 10 Example 11 Example 12 Example 13 Example 14 Example 15 Comparative Example 1 The component of Example 4 2 - - - - - - The component of Example 5 - 2 - - - - - The component of Example 6 - - 2 - - - - The component of Example 7 - - - 2 - - - The component of Example 8 - - - - 2 - - The component of Example 9 - - - - - 2 - Purified water Balance Balance Balance Balance Balance Balance Balance Potassium hydroxide 0.7 0.7 0.7 0.7 0.7 0.7 0.7 glycerin 10 10 10 10 10 10 10 Propylene glycol 5 5 5 5 5 5 5 Cetearyl alcohol 2 2 2 2 2 2 2 Stearic acid 15 15 15 15 15 15 15 Spices Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount antiseptic Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Examples 16 to 21 and Comparative Example 2: Production of lotion using a fermentation mixture of the plant extract of the present invention

Using the fermentation mixtures of black cumin, goup, bitter pepper, fenugreek mugwort, mulberry leaf mulberry or rhododendron mulberry produced in Examples 4 to 9, a lotion was prepared according to the prescription of the following Table 8 (unit: wt%) .

Example 16 Example 17 Example 18 Example 19 Example 20 Example 21 Comparative Example 2 The component of Example 4 0.2 - - - - - - The component of Example 5 - 0.2 - - - - - The component of Example 6 - - 0.2 - - - - The component of Example 7 - - - 0.2 - - - The component of Example 8 - - - - 0.2 - - The component of Example 9 - - - - - 0.2 - Purified water Balance Balance Balance Balance Balance Balance Balance ethanol 10 10 10 10 10 10 10 Polylauric acid
Polyoxyethylene sorbitan One One One One One One One glycerin 5 5 5 5 5 5 5 1,3-butylene glycol 6 6 6 6 6 6 6 Spices Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount antiseptic Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Examples 22 to 27 and Comparative Example 3: Preparation of an emulsion using a fermentation mixture of the plant extract of the present invention

The emulsions were prepared in the formulas shown in Table 9 below using the fermentation mixtures of black cumin, goup, bitter pepper, fenugreek, ganoderma, or rhododendron prepared in Examples 4 to 9 (unit: wt%).

Example 22 Example 23 Example 24 Example 25 Example 26 Example 27 Comparative Example 3 The component of Example 4 1.0 - - - - - - The component of Example 5 - 1.0 - - - - - The component of Example 6 - - 1.0 - - - - The component of Example 7 - - - 1.0 - - - The component of Example 8 - - - - 1.0 - - The component of Example 9 - - - - - 1.0 - Purified water Balance Balance Balance Balance Balance Balance Balance Vaseline 2 2 2 2 2 2 2 Sesquioleic acid
Sorbitan One One One One One One One Polyoxyethylene
Oleyl ethyl One One One One One One One glycerin 6 6 6 6 6 6 6 Carboxyvinyl polymer 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Tromethamine 0.16 0.16 0.16 0.16 0.16 0.16 0.16 Spices Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount antiseptic Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount

Experimental Example 2: Investigation of wrinkle reducing effect

The creams of Examples 10 to 15 and Comparative Example 1 were subjected to a panel test on the effect of improving skin wrinkles.

First, 140 healthy women from 35 to 50 years old were divided into 7 groups by 20 persons. The cream of Comparative Example 1 was applied to Group 1, the creams of Examples 2 to 7 were applied to the face of Group 2 to 7, Once a day for three months. After 3 months, the improvement of skin wrinkles was evaluated by the questionnaire survey. As to the improvement of the skin wrinkles and the elasticity of the subject, the three stages of no improvement, slight improvement and considerable improvement as compared with before use were determined, and the results are shown in Table 10 below.

division No improvement Slight improvement A significant improvement Example 10 One 4 15 Example 11 One 3 16 Example 12 One 3 16 Example 13 2 4 14 Example 14 One 4 15 Example 15 One 3 16 Comparative Example 1 10 8 2

As shown in Table 10, when the creams of Examples 10 to 15 according to the present invention were used, it was found that the effect of improving skin wrinkles and improving elasticity was excellent.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the invention as defined in the appended claims. It is obvious to those who have.

Claims (15)

A composition for promoting collagen synthesis, comprising as an active ingredient, a fermented product of at least one plant extract or a fraction thereof selected from the group consisting of Aspergillus oryzae, bitter pepper and prime minerals.
The composition for accelerating collagen synthesis according to claim 1, which further comprises a fermentation product of at least one plant selected from the group consisting of black cumin, and the like, and a fraction thereof.
The composition for accelerating collagen synthesis according to claim 1, wherein the fermented product is at least one selected from the group consisting of yeast fermented product, lactic acid fermented product and bifidobacterial fermented product of the plant extract or fraction thereof.
The composition for promoting collagen synthesis according to claim 1, wherein the total amount of the fermented product is 0.0001 to 20% by weight based on the total weight of the collagen synthesis promoting composition.
4. The method of claim 3 wherein the yeast is selected from the group consisting of a My process three Levy Jia as Saccharomyces (Saccharomyces cerevisiae), ski irradiation Caro My process ellipsometer Id (Schizosaccharomyces ellipsoids) and Saccharomyces My process bowl radio (Saccharomyces boulardii) in Wherein the composition is at least one selected from the group consisting of collagen,
4. The method of claim 3 wherein the Lactobacillus is Lactobacillus know also the filler's (Lactobacillus acidophilus), Lactobacillus casei (Lactobacillus casei), Lactobacillus Bulgaria kusu (Lactobacillus bulgaricus), Lactobacillus ramno suspension (Lactobacillus rhamnosus), Lactobacillus Flags lanthanide room (Lactobacillus plantarum), Lactobacillus ruteri (Lactobacillus reuteri), Lactobacillus konpu Versus (Lactobacillus confusus), Lactobacillus buffer momentum (Lactobacillus fermentum) and Lactobacillus brevis at least one member collagen is selected from the group consisting of (Lactobacillus brevis) Composition for promoting synthesis.
4. The method of claim 3 wherein the Bifidobacteria is Bifidobacterium breve (Bifidobacterium breve), Bifidobacterium bipyridinium Doom (Bifidobacterium bifidum), Bifidobacterium ronggeom (Bifidobacterium longum) and Bifidobacterium Infante tooth (Bifidobacterium infantis ). < / RTI >
Extracting at least one plant selected from the group consisting of Aspergillus oryzae; And
And fermenting the extract obtained in the extracting step into at least one strain selected from the group consisting of yeast, lactic acid bacteria and bifidus bacteria.
[9] The method of claim 8, wherein the fermentation step comprises fermenting the extract with yeast, lactic acid bacteria, and bifidobacteria, respectively, to obtain yeast fermented product, lactic acid fermented product and bifidobacterial fermented product; And a step of mixing the yeast fermented product, the lactic acid bacterium fermented product and the bifidobacterial fermented product to prepare a mixed fermented product.
A cosmetic composition for preventing or improving skin wrinkles containing the composition according to any one of claims 1 to 7 as an active ingredient.
A food composition for preventing or improving skin wrinkles containing the composition according to any one of claims 1 to 7 as an active ingredient.
A quasi-drug composition for preventing or improving skin wrinkles containing the composition according to any one of claims 1 to 7 as an active ingredient.
A pharmaceutical composition for preventing or treating skin aging comprising the composition according to any one of claims 1 to 7 as an active ingredient.
The pharmaceutical composition for preventing or treating skin aging according to claim 13, wherein the prevention or treatment of skin aging is carried out by preventing or improving skin wrinkles.
8. A method of preventing or improving skin wrinkles comprising the step of administering the composition of any one of claims 1 to 7 to a subject.