KR102484422B1 - Cosmetic composition comprising extract of Cactus and fermented product of Lactobacillus - Google Patents

Abstract

The present invention relates to a cosmetic composition which can be usefully used for improving skin conditions or preventing, alleviating or treating skin inflammatory diseases by comprising a desert cactus extract and a Lactobacillus strain culture solution. The cosmetic composition for improving skin conditions of the present invention includes: a desert cactus extract; and a Lactobacillus strain, a culture solution thereof, an extract of the culture solution, a lysate of the culture solution, or a mixture thereof, as active ingredients.

Description

Cosmetic composition comprising extract of Cactus and fermented product of Lactobacillus

It relates to a cosmetic composition.

Cactus is a plant that lives in the most barren environment on earth, and has the advantage of retaining water as it can survive for a long time without water.

Probiotics are live microorganisms that provide health benefits. Representative strains of probiotics include Lactobacillus sp. strains. Recently, studies to manufacture functional cosmetics by applying probiotics microorganisms to cosmetics have been actively conducted.

Accordingly, it is necessary to develop a new cosmetic product having a synergistic activity of improving skin condition of the mixture of the cactus and Lactobacillus strains.

One aspect is to provide a composition for improving skin condition comprising a desert cactus extract and a Lactobacillus strain, a culture medium thereof, an extract of the culture medium, a lysate or a mixture thereof as an active ingredient.

Another aspect is to provide a pharmaceutical composition for preventing or treating inflammatory skin diseases comprising a desert cactus extract and a Lactobacillus strain, a culture thereof, an extract of the culture solution, a lysate or a mixture thereof as an active ingredient.

One aspect provides a composition for improving skin conditions or preventing, improving or treating skin inflammatory diseases, comprising a desert cactus extract and a Lactobacillus strain, a culture medium thereof, an extract of the culture medium, a lysate or a mixture thereof as active ingredients.

The leaves, stems, flowers, roots, shells, fruits, or mixtures thereof of the desert cactus may be extracted. In one embodiment, the desert cactus may be a bogeom cactus (Opuntia ficus-indica), and may be used in combination with Baeknyeoncho.

The desert cactus extract may be obtained by extracting desert cactus with water, C1 to C4 lower alcohol, or a mixture thereof. The lower alcohol used for extraction of the desert cactus may be ethanol or methanol. During extraction, the dried desert cactus may be chopped, and then extracted by adding water, alcohol, or a mixture thereof in an amount of 2 to 20 times the weight of the cactus. The extraction temperature may be performed at 30 °C to 100 °C. Extraction time may be performed for 1 hour to 10 hours. As the extraction method, cooling, ultrasonic extraction, or reflux cooling extraction method may be used. The number of extractions may be repeated 1 to 5 times.

The extract may be included in an amount of 0.1 to 10% by weight based on the total weight of the composition. The extract is based on the total weight of the composition, for example, 0.1 to 10% by weight, 0.1 to 9% by weight, 0.1 to 7% by weight, 0.5 to 9% by weight, 0.5 to 5% by weight, 1 to 10% by weight, 1 to 8% by weight, 2 to 7% by weight or 3 to 6% by weight. At this time, when the content of the extract is less than the above range, there is a problem that skin aging, skin barrier strengthening, skin condition improvement such as skin wrinkles or skin elasticity, and / or inflammation prevention, improvement or treatment cannot be sufficiently exhibited. In addition, if it exceeds the above range, there is a problem that it is unsuitable for use as a raw material for cosmetics due to irritation to the skin, causing trouble and allergy, or due to the specific smell of the extract itself.

The Lactobacillus strain is a strain belonging to the genus Lactobacillus, for example, Lactobacillus plantarum , Lactobacillus acidophilus , Lactobacillus brevis , Lactobacillus casei ( Lactobacillus casei ), Lactobacillus curvatus ( Lactobacillus curvatus ), Lactobacillus delbrickii ( Lactobacillus delbrueckii ), Lactobacillus fermentum ( Lactobacillus fermentum ), Lactobacillus gasseri ( Lactobacillus gasseri ), Lactobacillus helveticus ), Lactobacillus paracasei , Lactobacillus pentosus , Lactobacillus reuteri , Lactobacillus rhamnosus , Lactobacillus sakei , Lactobacillus salivarius ( Lactobacillus salivarius ) and the like. In one embodiment, the Lactobacillus strain may be Lactobacillus plantarum.

As used herein, “culture medium” may be used interchangeably with “culture supernatant”, “conditioned medium” or “conditioned medium”, and refers to a medium capable of supplying nutrients so that Lactobacillus strains can grow and survive in vitro. It may refer to the entire medium including the strain obtained by culturing the strain for a certain period of time, its metabolites, extra nutrients, and the like. In addition, the culture solution may mean a culture solution obtained by removing the cells from the cell culture solution obtained by culturing the strain. On the other hand, the liquid from which the cells are removed from the culture solution is also called "supernatant", and the culture solution is left still for a certain period of time to take only the liquid of the upper layer except for the part sunk in the lower layer, remove the cells through filtration, or centrifuge the culture solution to It can be obtained by removing the precipitate and taking only the upper liquid. The "cell" refers to the strain itself of the present invention, and includes the strain itself isolated and selected from a skin sample or the like, or a strain isolated from a culture solution by culturing the strain. The cells can be obtained by centrifuging the culture solution and taking the part that has sunk to the lower layer, or it can be obtained by leaving it for a certain period of time and then removing the upper liquid because it sinks to the lower layer of the culture medium by gravity.

The culture solution may include a culture solution itself obtained by culturing the strain, a concentrate thereof, or a lyophilized product or a culture supernatant obtained by removing the strain from the culture solution, a concentrate thereof, or a lyophilisate.

The culture medium is obtained by culturing the Lactobacillus strain in a medium (eg, MRS medium, R2A medium or TSA medium) at a temperature above 10 ° C or below 40 ° C for a certain period of time, for example, 4 to 50 hours it could be

In one embodiment, the culture supernatant of the strain may be obtained by centrifuging or filtering the strain culture medium to remove the strain.

In another embodiment, the concentrate may be obtained by concentrating the supernatant obtained after filtering the strain culture medium itself, or the culture medium using a centrifugal separation or filter.

Culture medium and culture conditions for culturing the Lactobacillus strain can be appropriately selected or modified by those skilled in the art.

In this specification, the term "lysate" may mean a product obtained by disrupting the cell wall of the strain itself by chemical or physical force.

As used herein, the term "culture broth extract" refers to an extract obtained from the culture medium or a concentrate thereof, and may include an extract, a dilution or concentrate of the extract, a dried product obtained by drying the extract, or a crude or purified product thereof, or a fraction obtained by fractionating the same. can

The Lactobacillus strain, its culture medium, an extract of the culture medium, a lysate or a mixture thereof may be included in an amount of 0.1 to 12% by weight based on the total weight of the composition. The Lactobacillus strain, its culture medium, extract of the culture medium, lysate or a mixture thereof is, for example, 0.1 to 12% by weight, 0.1 to 10% by weight, 0.1 to 8% by weight, 0.5 to 9% by weight based on the total weight of the composition. %, 0.5 to 5% by weight, 1 to 10% by weight, 1 to 8% by weight, 2 to 7% by weight, 3 to 8% by weight or 4 to 12% by weight. At this time, when the content of the culture solution is less than the above range, there is a problem that skin aging, skin barrier strengthening, skin condition improvement such as skin wrinkles or skin elasticity, and / or inflammation prevention, improvement or treatment cannot be sufficiently exhibited. In addition, when the above range is exceeded, there is a problem that it is unsuitable for use as a raw material for cosmetics due to irritation to the skin, causing trouble and allergy, or due to the specific smell of the extract itself.

In one embodiment, the mixture of the desert cactus extract and the Lactobacillus plantarum strain culture medium increases collagen synthesis, expression of HAS3 and filaggrin, NO production and inflammatory activity compared to the desert cactus extract or the Lactobacillus plantarum strain culture medium alone. It was confirmed that the expression of cytokines was inhibited. Therefore, the composition according to one aspect includes a desert cactus extract and a Lactobacillus plantarum strain, a culture medium thereof, an extract of the culture medium, a lysate or a mixture thereof, thereby improving skin conditions or preventing, improving or treating inflammatory skin diseases synergistically. have an active

The skin condition improvement or skin beauty improvement may be suppression or improvement of skin aging, anti-wrinkle, skin barrier strengthening or skin moisturizing.

As used herein, the term "skin aging" refers to both tangible and intangible changes that appear in the skin with age, such as thinning of the epidermis, number of cells or blood vessels in the dermis, DNA damage repair ability, cell replacement cycle, It refers to reduction in wound healing, skin barrier function, epidermal moisture retention, sweat secretion, sebum secretion, vitamin D production, physical damage defense, chemical substance elimination ability, immune response, sensory function, and thermoregulation.

The composition may be used to improve skin aging caused by exogenous factors or endogenous factors. The extrinsic factor refers to various external factors, such as ultraviolet rays (light), and the endogenous factor is also referred to as a chronological factor and refers to a factor mainly caused by the passage of time. That is, the skin aging is not only premature aging symptoms induced by external stimuli such as ultraviolet rays, pollution, cigarette smoke, chemicals, etc., but also a natural aging phenomenon that occurs as the proliferation of skin cells decreases with age. It is a concept that includes wrinkles, loss of elasticity, skin sagging and dryness. In addition, wrinkles include stimulation caused by changes in internal and external factors that cause wrinkles by changing components constituting skin tissue.

The aging may be photoaging. The term "photoaging" is a phenomenon caused by external environmental factors, and the most representative factor is ultraviolet rays. Ultraviolet rays cause damage to biocomponents such as activation of proteolytic enzymes, chain scission of matrix proteins, and abnormal cross-linking, and repetition of these mechanisms results in apparent skin aging.

In this specification, the term "wrinkle" refers to a state in which elasticity of the skin is lost and loosened, and for example, the skin may be folded. The "prevention or improvement of skin wrinkles" may mean any action of preventing or improving wrinkles by suppressing the expression of wrinkle-related factors or increasing the total amount of collagen.

The "strengthen the skin barrier" may refer to any action that enhances the function of the skin barrier, which is located at the outermost layer of the skin and prevents loss of moisture and nutrients.

The "moisturizing skin" may refer to any action that maintains skin moisture or prevents moisture loss.

The "skin disease" may be a disease caused by impaired skin barrier function, skin aging, skin wound, skin scar, or skin inflammation. The term "prevention" includes inhibiting the development of a disease. The term "treatment" includes the inhibition, alleviation, or elimination of the development of a disease.

As used herein, the term "anti-inflammatory" may be used interchangeably with "inhibiting or improving inflammation", and may refer to any action that suppresses NO production by alleviating an immune response.

The skin barrier function impairment may refer to any change that occurs in the skin due to a decrease or damage of the skin barrier function. Examples include increased skin wrinkling, dryness, dermatitis, atopic dermatitis, allergic dermatitis, acne, and the like.

The skin inflammatory disease may be any one selected from the group consisting of skin wound, dermatitis, atopic dermatitis, pruritus, eczematous skin disease, dry eczema, erythema , urticaria , psoriasis, drug rash , and acne .

The desert cactus extract and the Lactobacillus strain, its culture solution, culture solution extract, lysate or a mixture thereof may be mixed in a weight ratio of 1:0.01 to 5. The desert cactus extract and Lactobacillus strain, its culture, the extract of the culture, the lysate or a mixture thereof, for example, 1:0.01 to 5, 1:0.01 to 0.05, 1:0.01 to 1, 1:0.1 to 5 , 1:0.1 to 3, 1:0.5 to 5, 1:0.5 to 3, 1:1 to 5 or 1:1.5 to 3 may be mixed in a weight ratio. At this time, when the mixing ratio of the desert cactus extract and the Lactobacillus strain, its culture medium, culture medium extract, lysate or a mixture thereof is less than the above range, skin aging, skin barrier strengthening, skin wrinkles or skin elasticity, etc. are improved. and/or inflammation prevention, amelioration, or treatment effects cannot be sufficiently exhibited. In addition, when the above range is exceeded, there is a problem that it is unsuitable for use as a raw material for cosmetics due to irritation to the skin, causing trouble and allergy, or due to the specific smell of the extract itself.

The composition is 0.001% to 80% by weight, for example, 0.01% to 60% by weight, 0.01% to 40% by weight, 0.01% to 30% by weight, 0.01% to 20% by weight, based on the total weight of the composition. %, 0.01% to 10%, 0.01% to 5%, 0.05% to 60%, 0.05% to 40%, 0.05% to 30%, 0.05% to 20%, 0.05% to 10% by weight, 0.05% to 5% by weight, 0.1% to 60% by weight, 0.1% to 40% by weight, 0.1% to 30% by weight, 0.1% to 20% by weight, 0.1% by weight % to 10% by weight, or 0.1% to 5% by weight of a desert cactus extract; And it may include a mixture of a Lactobacillus strain, its culture medium, an extract of the culture medium, a lysate or a mixture thereof. At this time, when the content of the mixture is less than the above range, there is a problem in that the skin condition improvement effect, for example, skin aging inhibition, wound improvement, skin barrier strengthening, skin wrinkle inhibition, or skin moisturizing effect is not sufficiently exhibited.

As used herein, the term "included as an active ingredient" refers to the desert cactus extract of the present specification to the extent that it can exhibit the above-mentioned effects; And means that a Lactobacillus strain, its culture medium, culture medium extract, lysate or a mixture thereof is added, and various ingredients are added as subcomponents for drug delivery and stabilization, etc. to be formulated in various forms. it means to

In another embodiment, the composition may be a cosmetic composition.

The cosmetic composition may have, for example, softening lotion, nutrient lotion, massage cream, nutrient cream, essence, pack, gel, ampoule, or skin-adhesive cosmetic formulation.

Ingredients included in the cosmetic composition may include ingredients commonly used in cosmetic compositions other than the composition as active ingredients, for example, conventional adjuvants and carriers such as stabilizers, solubilizers, vitamins, pigments and flavors. can include

In another embodiment, the composition may be a composition for external application for skin.

In the present specification, the external skin preparation may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal delivery patch, drug-containing bandage, lotion, or a combination thereof. The external skin preparation is a component usually used in external preparations for skin such as cosmetics or pharmaceuticals, for example, water-based components, oil-based components, powder components, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, and fragrances. , colorants, various skin nutrients, or combinations thereof and may be suitably blended as needed. The external skin preparations include metal sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, bellapamil, licorice extract, glabridin, and calin. Hot-water extracts of fruits, various herbal medicines, tocopherol acetate, glycyrrhizic acid, tranexamic acid and its derivatives or salts and other drugs, vitamin C, magnesium ascorbate phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, Sugars, such as trehalose, etc. can also be mix|blended suitably.

In another embodiment, the composition may be a pharmaceutical composition.

The pharmaceutical composition may additionally include a pharmaceutically acceptable diluent or carrier. The diluent may be lactose, corn starch, soybean oil, microcrystalline cellulose, or mannitol, and magnesium stearate, talc, or a combination thereof as a lubricant. The carrier may be an excipient, a disintegrant, a binder, a lubricant, or a combination thereof. The excipient may be microcrystalline cellulose, lactose, low-substituted hydroxycellulose, or a combination thereof. The disintegrant may be calcium carboxymethylcellulose, sodium starch glycolate, calcium monohydrogen phosphate, or a combination thereof. The binder may be polyvinylpyrrolidone, low-substituted hydroxypropylcellulose, hydroxypropylcellulose, or a combination thereof. The lubricant may be magnesium stearate, silicon dioxide, talc, or a combination thereof.

The pharmaceutical composition may be formulated for oral or parenteral administration. Oral dosage forms may be granules, powders, solutions, tablets, capsules, dry syrups, or combinations thereof. Parenteral dosage forms may be injections.

In another embodiment, the composition may be a health functional food composition.

The health functional food composition may be used alone or in combination with the strain or its culture medium or other food or food component, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, when preparing food or beverage, the composition of the present specification may be added in an amount of 15 parts by weight or less based on the raw material. There is no particular limitation on the type of health functional food. Among the types of health functional foods, beverage compositions may contain various flavoring agents or natural carbohydrates as additional components, like conventional beverages. The natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as thaumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used. The health food composition may also contain nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, and carbonated beverages. carbonation agent used, or a combination thereof. The health functional food composition may also contain natural fruit juice, fruit juice beverages, fruit flesh for preparing vegetable beverages, or a combination thereof.

Another aspect also provides a method of preventing, ameliorating, or treating a condition in a subject comprising treating or administering to a subject in need thereof an effective amount of a composition described above.

The condition of the subject may be a skin-related condition or an inflammation-related condition.

As used herein, the terms "administering," "introducing," and "implanting" are used interchangeably and according to one embodiment into a subject by a method or route that results in at least partial localization of a composition to a desired site. It may mean the arrangement of the composition according to one embodiment of.

Administration may be administered by a method known in the art. Administration can be administered directly to a subject by any means, for example, by routes such as intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal or subcutaneous administration. can The administration may be administered systemically or locally.

The subject may be a mammal, such as a human, cow, horse, pig, dog, sheep, goat, or cat. The subject may be a subject in need of improving skin beauty, for example, moisturizing the skin, strengthening the skin barrier, inhibiting skin inflammation, and improving skin wrinkles.

The administration is 0.1 mg to 1,000 mg per day of the composition according to one embodiment, for example, 0.1 mg to 500 mg, 0.1 mg to 100 mg, 0.1 mg to 50 mg, 0.1 mg to 25 mg, 1 mg to 1,000 mg, 1 mg to 500 mg, 1 mg to 100 mg, 1 mg to 50 mg, 1 mg to 25 mg, 5 mg to 1,000 mg, 5 mg to 500 mg, 5 mg to 100 mg, 5 mg to 50 mg , 5 mg to 25 mg, 10 mg to 1,000 mg, 10 mg to 500 mg, 10 mg to 100 mg, 10 mg to 50 mg, or 10 mg to 25 mg may be administered. However, the dosage may be prescribed in various ways depending on factors such as formulation method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration route, excretion rate and reaction sensitivity, and those skilled in the art can Dosage can be appropriately adjusted in consideration of these factors. The number of administrations can be once a day or two or more times within the range of clinically acceptable side effects, and administration can be performed at one or two or more sites, daily or at intervals of 2 to 5 days. The number of administration days may be administered from 1 day to 30 days per treatment. If necessary, the same treatment can be repeated after a titration period. For non-human animals, the same dosage per kg as for humans is used, or the above dosage is converted by the volume ratio (eg, average value) of the organ (heart, etc.) between the target animal and the human. A single dose can be administered.

The composition according to one aspect may be usefully used for preventing, improving, or treating skin conditions or skin inflammatory diseases by including a desert cactus extract and a Lactobacillus strain culture medium.

1 is a result showing the effect of a mixture of a desert cactus extract and a Lactobacillus strain culture solution according to one embodiment on the production of collagen.
Figure 2 is a result showing the effect of the mixture of the desert cactus extract and the Lactobacillus strain culture medium on the expression of HAS3 according to one embodiment.
Figure 3 is a result showing the effect of the mixture of the desert cactus extract and the Lactobacillus strain culture solution according to one embodiment on the expression of filaggrin.
Figure 4 is a result showing the effect of the mixture of the desert cactus extract and the Lactobacillus strain culture solution according to one embodiment on the production of NO.
5 is a result showing the effect of a mixture of a desert cactus extract and a Lactobacillus strain culture medium on the production of TNF-α according to one embodiment.
6 is a result showing the effect of a mixture of a desert cactus extract and a Lactobacillus strain culture medium on the expression of IL-6 according to one embodiment.

Hereinafter, a preferred embodiment is presented to aid understanding of the present invention. However, the following examples are provided to more easily understand the present invention, and the content of the present invention is not limited by the following examples.

[Production Example]

Preparation Example 1. Preparation of desert cactus extract

1,000 mL of purified water was added to 20 g of Opuntia ficus-indica, one of the desert cacti, and extracted at 60 °C for 12 hours. Thereafter, the raw material was removed using an 8 μm filter and filtered through a 0.45 μm membrane filter to prepare a desert cactus extract.

Preparation Example 2. Preparation of Lactobacillus culture medium

1,000 mL of purified water was added to 55 g of the MRS medium, autoclaved at 121 °C for 15 minutes, and then cooled to room temperature. Thereafter, the Lactobacillus plantarum strain isolated from kimchi was inoculated to 2% of the volume of the culture bag, and then cultured for 48 hours at 180 rpm and 37 °C. The culture solution was centrifuged at 4,500 rpm for 20 minutes to remove cells, and the supernatant was obtained and filtered through a 0.45 μm membrane filter to prepare a lactobacillus fermentation filtrate.

[Example]

Examples 1-3. Preparation of cosmetic composition

A cosmetic composition containing a desert cactus extract and a Lactobacillus culture medium was prepared. Specifically, a cosmetic composition containing the ingredients and contents of Table 1 below was prepared. In this specification, unless otherwise indicated, content is % by weight.

Example 1 Example 2 Example 3 Purified water 73.0 73.0 73.0 Preparation Example 1 7.0 5.0 3.0 Preparation Example 2 3.0 5.0 7.0 Butylene Glycol 10.0 10.0 10.0 Dipropylene Glycol 5.0 5.0 5.0 1,2-Hexanediol 2.0 2.0 2.0 Sum 100 100 100

[Comparative Example]

Comparative Examples 1-2. Preparation of cosmetic composition

A cosmetic composition containing a desert cactus extract or a Lactobacillus culture medium was prepared. Specifically, a cosmetic composition containing the components and contents of Table 2 below was prepared.

Comparative Example 1 Comparative Example 2 Purified water 73.0 73.0 Preparation Example 1 10.0 - Preparation Example 2 - 10.0 Butylene Glycol 10.0 10.0 Dipropylene Glycol 5.0 5.0 1,2-Hexanediol 2.0 2.0 Sum 100 100

[Experimental example]

Check the effect of improving skin wrinkles and elasticity

The effect of improving skin wrinkles and elasticity of the cosmetic composition according to one aspect was confirmed. Specifically, CCD-98sk cells, a human fibroblast, were inoculated into a 24-well plate at a concentration of 2×10 ⁵ cells/well and cultured for 24 hours. Thereafter, the cells were washed twice with PBS, and the Examples 1-3 and Comparative Examples 1-2 were treated at each concentration (0.3% (w/w), 1.0% (w/w)) for 48 hours. during further incubation. TGF-β1 ng/ml was used as a positive control. Then, after collecting the culture medium from each sample, the collagen content was measured using a procollagen type I C-peptide assay kit (Takara Bio Inc.).

1 is a result showing the effect of a mixture of a desert cactus extract and a Lactobacillus strain culture solution according to one embodiment on the production of collagen.

As a result, as shown in Figure 1, Examples 1 to 3 was confirmed that the production of procollagen increased compared to Comparative Examples 1 to 2. In particular, it was confirmed that the production of procollagen significantly increased in Example 1.

Therefore, the composition according to one aspect has a synergistic activity of collagen production by including the desert cactus extract and the Lactobacillus strain culture medium, and thus can be usefully used for anti-aging, such as improving skin wrinkles and elasticity.

Confirmation of skin moisturizing and barrier improvement effect

The skin moisturizing and barrier improving effects of the cosmetic composition according to one aspect were confirmed. Specifically, HaCaT cells, a human keratinocyte, were inoculated into a 6-well plate at a concentration of 2×10 ⁵ cells/well and cultured for 24 hours. Thereafter, the cells were washed twice with PBS, and the Examples 1-3 and Comparative Examples 1-2 were treated at each concentration (0.3% (w/w), 1.0% (w/w)) for 24 hours. during further incubation. As a positive control, 5 µM of retinoic acid or 2 mM of CaCl ₂ was used. After that, RNA was isolated from the cells of each sample using Trizol (RNA iso, DAKARA, Japan), RNA was quantified at 260 nm by nanodrop, and cDNA was synthesized in an amplifier using 2 μg of RNA each. (C1000 Thermal Cycler, Bio-Rad, USA). Using the synthesized cDNA as a template, cybergreen (SYBR Green supermix, Applied Biosystems, USA) was added together with primers and cDNA for HAS3 (hyaluronic acid synthase) and filagrin, which are factors related to skin barrier strengthening and moisturizing. Real-time polymerase chain reaction was performed in a (real-time) PCR machine (Step One Plus, Applied Biosystems, USA).

2 is a result showing the effect of a mixture of a desert cactus extract and a Lactobacillus strain culture medium on the expression of HAS3 according to one embodiment.

Figure 3 is a result showing the effect of the mixture of the desert cactus extract and the Lactobacillus strain culture solution according to one embodiment on the expression of filaggrin.

As a result, as shown in FIGS. 2 and 3, it was confirmed that Examples 1-3 significantly increased the expression of HAS3 and filaggrin compared to Comparative Examples 1-2. Specifically, it was confirmed that the expression of HAS3 and filaggrin significantly increased in Example 1.

Therefore, the composition according to one aspect has a synergistic activity of HAS3 and filaggrin expression by including a desert cactus extract and a Lactobacillus strain culture medium, and thus can be usefully used for skin moisturizing and barrier improvement.

Confirmation of anti-inflammatory activity

The anti-inflammatory activity of the composition according to one aspect was confirmed. Specifically, Raw264.7 cells, a macrophage cell line, were inoculated in a 6-well plate at a concentration of 2×10 ⁵ cells/well to attach the cells, and then cultured for 24 hours. Thereafter, Examples 1 to 3 and Comparative Examples 1 to 2 were treated with concentrations (1.0% (w/w), 2.0% (w/w)) and further cultured for 24 hours. As a positive control, 100 ng/ml of LPS was used. Then, after collecting the supernatant from each sample, the NO expression level was measured with a microplate reader using Griess reagent. In addition, the expression levels of inflammatory cytokines were measured using TNF-α ELISA kit and IL-6 ELISA kit (R&D).

Figure 4 is a result showing the effect of the mixture of the desert cactus extract and the Lactobacillus strain culture medium on the production of NO according to one embodiment.

5 is a result showing the effect of a mixture of a desert cactus extract and a Lactobacillus strain culture medium on the production of TNF-α according to one embodiment.

6 is a result showing the effect of a mixture of a desert cactus extract and a Lactobacillus strain culture medium on the expression of IL-6 according to one embodiment.

As a result, as shown in FIG. 1 , it was confirmed that the production of NO in Example 1 was remarkably low compared to Comparative Examples 1 and 2. In addition, as shown in Figures 2 and 3, Example 1 was confirmed to significantly reduce the expression of inflammatory cytokines TNF-α and IL-6 compared to Comparative Examples 1 and 2.

Therefore, the composition according to one aspect has a synergistic activity of inhibiting the production of NO and the expression of inflammatory cytokines by including the desert cactus extract and the Lactobacillus strain culture, and thus can be usefully used for preventing, improving or treating inflammatory skin diseases. .

The above description of the present invention is for illustrative purposes, and those skilled in the art can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting.

Claims (9)

desert cactus extract; And a cosmetic composition for improving skin conditions comprising a Lactobacillus strain, its culture medium, an extract of the culture medium, a lysate or a mixture thereof as an active ingredient. The cosmetic composition according to claim 1, wherein the desert cactus extract is obtained by extracting desert cactus with water, C1 to C4 lower alcohol, or a mixture thereof. The method according to claim 1, wherein the Lactobacillus strain is Lactobacillus plantarum , Lactobacillus acidophilus , Lactobacillus brevis , Lactobacillus casei , Lactobacillus curba Tooth ( Lactobacillus curvatus ), Lactobacillus delbrickii ( Lactobacillus delbrueckii ), Lactobacillus fermentum ( Lactobacillus fermentum ), Lactobacillus gasseri ( Lactobacillus gasseri ), Lactobacillus helveticus ( Lactobacillus helveticus ), Lactobacillus paracasei ( Lactobacillus paracasei ), Lactobacillus pentosus , Lactobacillus reuteri , Lactobacillus rhamnosus , Lactobacillus sakei , and Lactobacillus salivarius A cosmetic composition that is selected from. The cosmetic composition according to claim 1, wherein the desert cactus extract is included in an amount of 0.1 to 10% by weight based on the total weight of the composition. The cosmetic composition according to claim 1, wherein the Lactobacillus strain, its culture medium, an extract of the culture medium, a lysate or a mixture thereof is contained in an amount of 0.1 to 12% by weight based on the total weight of the composition. The cosmetic composition according to claim 1, wherein the desert cactus extract and the Lactobacillus strain, its culture medium, culture medium extract, lysate or a mixture thereof are mixed in a weight ratio of 1:0.01 to 5. The cosmetic composition according to claim 1, wherein the skin condition improvement is skin aging, skin barrier, skin moisturizing, wrinkle improvement or skin elasticity. desert cactus extract; And a pharmaceutical composition for preventing or treating inflammatory skin diseases comprising a Lactobacillus strain, its culture medium, an extract of the culture medium, a lysate or a mixture thereof as an active ingredient. The method according to claim 8, wherein the skin inflammatory disease is any one selected from the group consisting of skin wound, dermatitis, atopic dermatitis, pruritus, eczematous skin disease, dry eczema, erythema, urticaria, psoriasis, drug rash, and acne. enemy composition.

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