KR102367436B1 - Composition comprising mixed fermented product of young trifoliate orange, young green tea and bitter orange flower, and its use for improving skin condition - Google Patents

Abstract

The present invention relates to a composition comprising a mixed fermented material of young Poncirus trifoliata, young Camellia sinensis, and Citrus × aurantium flower, and to a use thereof for improving the skin condition. The composition comprising a mixed fermented material of young Poncirus trifoliata, young Camellia sinensis, and Citrus × aurantium flower according to one aspect of the present invention can be effectively used for preventing, alleviating, or treating skin-related conditions, such as skin aging prevention, skin wrinkle improvement, skin elasticity enhancement, skin inflammation alleviation, skin soothing, and the like.

Description

Composition comprising mixed fermented product of young trifoliate orange, young green tea and bitter orange flower, and its use for improving skin condition

It relates to a cosmetic composition, a food composition, a pharmaceutical composition, and a use for improving skin condition, comprising a mixed fermented product of young taengjae, young green tea and bitter orange flower.

Functional cosmetics are cosmetics with enhanced specific functions, such as skin whitening, wrinkle improvement, UV protection, acne relief, atopy relief, and skin soothing. As not only products specialized for one function but also multifunctional cosmetics with various functions have been developed, the ease of use is increasing.

However, when chemical ingredients are added to give functionality to cosmetics, they have a rather adverse effect on the skin, and thus interest in the development of natural cosmetics is increasing recently. Natural cosmetics are cosmetics using raw materials derived from animals and plants, and have the advantage of being safe and non-irritating to the skin. As an example of natural cosmetics, cosmetics using various plant mixed extracts have been developed (Patent Document 1).

Therefore, there is a need for continuous development of ingredients derived from natural products that can be usefully used for skin-related conditions.

KR 10-0967018 B1

It provides a composition comprising a mixed fermented product of young tangja, young green tea, and bitter orange flower.

A method for preparing the fermented product is provided.

It provides a method for improving the skin condition of a subject, comprising administering to a subject in need thereof an effective amount of the fermented product or a composition comprising the same.

Provided is the use of the fermented product for use in preparing a composition for improving skin conditions.

Provided is the use of the fermented product for use in the manufacture of a medicament for the prevention or treatment of skin inflammatory diseases.

One aspect provides a composition comprising a mixed fermented product of young taengjae, young green tea and bitter orange flower.

The term "trifoliate orange" refers to the fruit of the tangerine tree ( Poncirus trifoliata ). "Young taengja" refers to when taengja is green before ripening to yellow, and is also called "jisil".

The term "green tea" generally refers to tea leaves or tea made from tea leaves. "Young green tea" refers to the young leaves of the tea tree.

The term " Citrus aurantium var. amara (Bitter Orange) Flower" means the flower of bitter orange.

The fermented product may be obtained by fermenting a mixture containing young taengjae, young green tea and bitter orange flowers.

The fermented product may be a mixed fermented product of young taengja extract, young green tea extract, and bitter orange flower extract. The extract may be extracted from each plant source using a conventional extraction solvent according to a known method. The extraction solvent may be water, alcohol, a combination thereof, or the like, but is not limited thereto. For example, the extract may be a hot water extract, but is not limited thereto.

The mixing weight ratio or volume ratio of the young taengja, young green tea and bitter orange flower is not limited to a specific weight ratio or volume ratio. In one embodiment, the fermented product is 1 to 100:1 to 100:1 to 100, 1 to 50:1 to 50:1 to 50, 1 to 20: 1 to 20:1 to 20, 1 to 10:1 to 10:1 to 10, or 1 to 5:1 to 5:1 to 5, for example, 3:1:1 by volume It may be obtained by

The fermented product can be obtained by fermenting young taengja, young green tea and bitter orange flowers with microorganisms.

The microorganism may be one or more microorganisms selected from the group consisting of Lactobacillus sp. and Bacillus sp. , but is not limited thereto.

The Lactobacillus genus ( Lactobacillus sp. ) The microorganism is not limited to a specific type, but, for example, Lactobacillus acidophilus ( L. acidophilus ), Lactobacillus casei ( L. casei ), Lactobacillus gasseri ( L gasseri ), Lactobacillus Delbruecki bulgaricus ( L. delbrueckii ssp. bulgaricus ), Lactobacillus helveticus ( L. helveticus ), Lactobacillus fermentum ( L. fermentum ), Lactobacillus paracasei ( L. paracasei ) ), Lactobacillus plantarum ( L. plantarumm ), Lactobacillus reuteri ( L. reuteri ), Lactobacillus rhamnosus ( L. rhamnosus ), and Lactobacillus salivarius ( L. salivarius ) It may be selected from the group consisting of there is.

The Bacillus genus ( Bacillus sp. ) Microorganisms include both aerobic or facultative anaerobic bacteria widely distributed in nature. The Bacillus genus microorganism is not limited to a specific type, but, for example, Bacillus subtilis ( B. subtilis ), Bacillus coagulans ( B. coagulans ), Bacillus velezensis ( B. velezensis ), Bacillus lichenifor Miss ( B. licheniformis ), Bacillus polyfermenticus ( B. polyfermenticus ), Bacillus methylotrophicus ( B. methylotrophicus ), and Bacillus amyloliquefaciens ( B. amyloliquefaciens ) It may be selected from the group consisting of.

As the fermentation conditions, an appropriate temperature and time may be selected according to the type of microorganism. For example, the fermentation may be carried out at 20 to 40 ℃, or 30 to 40 ℃, but is not particularly limited thereto. In addition, the fermentation may be performed for 1-200 hours, 1-180 hours, 24-200 hours, 24-180 hours, 24-120 hours, 48-200 hours, 48-180 hours, or 48-120 hours. , which is not particularly limited thereto.

The fermentation medium may be appropriately selected according to the type of microorganism. As the medium, a known medium or a conventional medium capable of culturing the microorganism may be used depending on the type of the microorganism. For example, the medium may include one or a mixture of two or more selected from glucose, yeast extract, malt extract, peptone, glutamic acid, K ₂ HPO ₄ , KH ₂ PO ₄ , NaCl, but is not limited thereto. .

In one embodiment, when the microorganism is a microorganism of the genus Lactobacillus, the medium may include glucose, peptone, and yeast extract.

In another embodiment, when the microorganism is a microorganism of the genus Bacillus, the medium may include glucose, peptone, yeast extract, glutamic acid, K ₂ HPO ₄ , and KH ₂ PO ₄ .

The fermented product may be a fermented broth obtained by fermenting young green tea and bitter orange flowers with microorganisms, a diluted or concentrated solution of the fermented broth, a dried product obtained by drying the fermented broth, or these prepared or purified products, and a fraction obtained by fractionation thereof. and the like.

The fermented product is 0.0001 to 99.9999% (v / v), 0.0001 to 90% (v / v), 0.0001 to 50% (v / v), 0.0001 to 10% (v / v), 0.001 to 99.9999 compared to the total composition % (v/v), 0.001 to 90% (v/v), 0.001 to 50% (v/v), 0.001 to 10% (v/v), 0.01 to 99.9999% (v/v), 0.01 to 90 % (v/v), 0.01 to 50% (v/v), 0.01 to 20% (v/v), 0.01 to 10% (v/v), 0.1 to 99.9999% (v/v), 0.1 to 90 % (v/v), 0.1 to 50% (v/v), 0.1 to 20% (v/v), or 0.1 to 10% (v/v) may be included, but is not particularly limited thereto.

The term "included as an active ingredient" means that the fermented product of the present specification is added to an extent capable of exhibiting the above-mentioned effects. In addition, this may include being formulated in various forms by adding various components as sub-components for drug delivery and stabilization.

The composition may be a composition for improving skin conditions, improving skin beauty, preventing, improving or treating skin diseases.

In one embodiment, the composition may be for improving skin condition.

The skin condition improvement may be skin aging prevention, skin wrinkle improvement, skin elasticity enhancement, skin inflammation improvement, skin soothing, or a combination of two or more thereof.

The term "skin aging" refers to the tangible and intangible changes that appear on the skin with age, such as a thinning of the epidermis, the number of cells or blood vessels in the dermis, DNA damage repair ability, cell replacement cycle, wound healing, It refers to a decrease in skin barrier function, epidermal moisture retention, sweat secretion, sebum secretion, vitamin D production, physical damage protection, chemical removal ability, immune response, sensory function, and thermoregulation.

The fermented product may be for preventing skin aging caused by extrinsic factors or intrinsic factors, improving skin aging, or for anti-aging. The extrinsic factor refers to various external factors, such as ultraviolet light (light). The intrinsic factor is also referred to as a chronological factor, and refers to a factor mainly caused by the passage of time. That is, the skin aging specifically refers to not only the premature aging symptoms induced by external stimuli such as ultraviolet rays, pollution, cigarette smoke, chemical substances, etc., but also a natural aging phenomenon that occurs as the proliferation of skin cells decreases with aging. , that is, it is a concept that includes skin aging due to age, and includes all of wrinkles, loss of elasticity, skin sagging and dryness. In addition, wrinkles include those that cause wrinkles by stimulation by changes in internal and external factors by changing the components constituting the skin tissue.

The aging may be photoaging. The term “photoaging” is a phenomenon induced by external environmental factors, and the most representative factor is ultraviolet rays. Ultraviolet rays cause damage to biological components such as activation of proteolytic enzymes, chain cleavage of matrix proteins, and abnormal cross-linking, and repetition of these mechanisms leads to apparent skin aging. Accordingly, the composition may be for preventing, improving, or treating photoaging caused by ultraviolet B (UVB).

The term "skin wrinkle improvement" is interpreted to include not only an action of reducing the number or depth of skin wrinkles, but also inhibiting skin aging through wrinkle improvement.

The term “enhancement of skin elasticity” may refer to any action that increases skin elasticity, which is reduced due to aging, or prevents deterioration of skin elasticity.

The term “skin inflammation” refers to an inflammation of the skin.

The term “improving skin inflammation” may be used interchangeably with “anti-inflammatory” and refers to any action that reduces or ameliorates skin inflammation, or prevents or inhibits the occurrence of skin inflammation.

The term "skin soothing" refers to any action that relieves and stabilizes the heat or pain of irritated skin. The skin soothing may include soothing the skin sensitized by inflammation.

The term “skin disease” may be a disease caused by skin aging or an inflammatory disease of the skin. The term “prevention” includes inhibiting the development of a disease. The term “treatment” includes inhibiting, alleviating, or eliminating the development of a disease.

Details of the skin aging are the same as described above. Accordingly, the skin aging may be photoaging or skin aging due to age.

The skin inflammatory disease may be any one selected from the group consisting of skin wounds, dermatitis, atopic dermatitis, pruritus, eczematous skin disease, dry eczema, erythema, urticaria, psoriasis, weak rash, and acne.

The composition may increase the expression of procollagen. In general, it is known that collagen related to wrinkles of skin cells is synthesized in the form of a precursor called procollagen. Therefore, by measuring the amount of the procollagen peptide, it is possible to grasp the degree of collagen synthesis in the cell. The composition exhibits an effect of improving skin wrinkles and enhancing skin elasticity by increasing the expression of procollagen, and thus may exhibit an anti-aging effect on the skin.

The composition may inhibit nitric oxide production or inhibit the expression of TNF-α. Accordingly, the composition may exhibit a skin inflammation improvement and skin soothing effect.

Accordingly, the composition may increase the expression of procollagen, inhibit nitric oxide production, or inhibit the expression of TNF-α.

In one embodiment, it was confirmed that the fermented product has excellent skin wrinkle improvement effect, skin elasticity enhancing effect, skin aging prevention effect, anti-inflammatory effect and skin soothing effect. Specifically, the mixed fermented product of Young Taengja, young green tea, and bitter orange flower extracts, respectively, or extracts containing all of them, improves skin wrinkle, improves skin elasticity, and improves skin aging. It was confirmed that the prevention effect, anti-inflammatory effect and skin soothing effect were excellent. In addition, when the microorganisms of the genus Lactobacillus and the genus Bacillus were used as fermenting microorganisms, the effects were excellent, but especially, when the microorganisms of the genus Lactobacillus were used, the most excellent effect was exhibited.

The composition may be a cosmetic composition.

The cosmetic composition may be prepared in any conventionally prepared formulation. For example, the cosmetic composition may be a lotion, cream, essence, cleansing foam, cleansing water, pack, ampoule, body lotion, body oil, body gel, shampoo, conditioner, hair conditioner, hair gel, foundation, lipstick, mascara, makeup It may have a cosmetic formulation of a base or skin adhesion type. The skin adhesion type cosmetic formulation may be, for example, a mask pack, but is not limited thereto.

Components included in the cosmetic composition may include components commonly used in cosmetic compositions in addition to the composition as an active ingredient, for example, conventional adjuvants and carriers such as stabilizers, solubilizers, vitamins, pigments and fragrances. may include

In addition, the composition may be a composition for external application to the skin.

The external preparation for skin may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal patch, drug-containing bandage, lotion, or a combination thereof. The external preparation for skin is a component usually used in external preparations for skin such as cosmetics or pharmaceuticals, for example, an aqueous component, an oily component, a powder component, alcohol, a moisturizer, a thickener, an ultraviolet absorber, a whitening agent, a preservative, an antioxidant, a surfactant, a fragrance , colorant, various skin nutrients, or a combination thereof may be appropriately formulated as needed. The external preparation for skin includes metal-blocking agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid, caffeine, tannin, belapamil, licorice extract, glablidine, and kaline. Fruit hot water extracts, various herbal medicines, tocopherol acetate, glitylittic acid, tranexamic acid and derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, Sugars, such as trehalose, etc. can be mix|blended suitably.

The composition may be a food composition. The composition may be a health functional food composition.

The food composition may be used alone or in combination with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use (prophylactic, health or therapeutic treatment). There is no particular limitation on the type of the health functional food. Among the types of health functional food, the beverage composition may contain various flavoring agents or natural carbohydrates as an additional component like a conventional beverage. The natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as taumartin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like can be used. The health food composition can also be added to nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonated beverages carbonation agent used, or a combination thereof. The health functional food composition may also contain natural fruit juice, fruit juice beverage, fruit flesh for the production of vegetable beverage, or a combination thereof.

The composition may be a pharmaceutical composition.

In one embodiment, the pharmaceutical composition may be for preventing or treating skin inflammatory diseases.

The pharmaceutical composition may further include a pharmaceutically acceptable diluent or carrier. The diluent may be lactose, corn starch, soybean oil, microcrystalline cellulose, or mannitol, and the lubricant may be magnesium stearate, talc, or a combination thereof. The carrier may be an excipient, a disintegrant, a binder, a lubricant, or a combination thereof. The excipient may be microcrystalline cellulose, lactose, low-substituted hydroxycellulose, or a combination thereof. The disintegrant may be carboxymethyl cellulose calcium, sodium starch glycolate, anhydrous calcium monohydrogen phosphate, or a combination thereof. The binder may be polyvinylpyrrolidone, low-substituted hydroxypropylcellulose, hydroxypropylcellulose, or a combination thereof. The lubricant may be magnesium stearate, silicon dioxide, talc, or a combination thereof.

The pharmaceutical composition may be formulated as an oral or parenteral dosage form. Oral dosage forms may be granules, powders, solutions, tablets, capsules, dry syrups, or a combination thereof. The parenteral dosage form may be an injection.

Another aspect provides a method for producing a complex fermented product of young tangja, young green tea and bitter orange flower.

The method includes the step of incubating a combination of young dandelion seeds, young green tea and bitter orange flowers with microorganisms to obtain a fermented product.

The step of obtaining the fermented product includes: adding young taengja, young green tea and bitter orange flowers to the medium and sterilizing; and inoculating and incubating the medium with microorganisms to obtain a fermented product.

Details of the young taengja, young green tea, bitter orange flower, medium, microorganism, and fermented product are as described above.

The young taengja, young green tea, and bitter orange flower may be added in an amount of 0.01 to 20% (v/v), or 0.1 to 10% (v/v), respectively, based on the total volume of the medium, but is not limited thereto.

The sterilization may be performed using a known conventional method.

The microorganism may be used in an amount sufficient to allow fermentation to occur, which may be appropriately selected by those skilled in the art in consideration of the amount of fermentation raw materials.

The incubation temperature and time may be selected as sufficient temperature and time for fermentation to occur, which may be appropriately selected by those skilled in the art in consideration of the amount of fermentation raw material, the type of microorganism, and the like. For example, the incubation may be performed at 20 to 40° C., but is not particularly limited thereto. In addition, the incubation may be performed for 1 to 200 hours, 1 to 180 hours, 24 to 200 hours, 24 to 180 hours, 24 to 120 hours, 48 to 200 hours, 48 to 180 hours, or 48 to 120 hours. , which is not particularly limited thereto.

The fermentation may be performed in a medium capable of culturing microorganisms. As the medium, a known medium or a conventional medium capable of culturing the microorganism may be used depending on the type of the microorganism. Exemplary medium components according to the type of microorganism are as described above.

The method may further include isolating the cells from the fermented product. For the isolation of the cells, a known conventional method may be used, for example, stationary, centrifugation, a combination thereof, and the like may be used.

The method may further include filtering the fermented product. The filtration may use a known conventional method.

Another aspect provides a method for improving a skin condition in a subject comprising administering to a subject in need thereof an effective amount of the fermented product or a composition comprising the same.

Details of the fermented product, composition, and skin condition improvement are the same as described above.

The term "effective amount" means an amount effective enough to produce the above-mentioned effects.

The terms "administering", "introducing", and "implanting" are used interchangeably and in one embodiment into a subject by a method or route that results in at least partial localization of the composition according to one embodiment to a desired site. It may mean the arrangement of the composition according to

Administration may be administered by methods known in the art. Administration can be administered directly to a subject by any means, for example, intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal or subcutaneous administration. can The administration may be systemically or locally. The administration may be applied to the skin.

The subject may be a mammal, such as a human, cow, horse, pig, dog, sheep, goat, or cat. The subject may be an individual in need of improving skin condition, for example, preventing skin aging, improving skin wrinkles, enhancing skin elasticity, improving skin inflammation, or soothing skin.

The dosage may be prescribed in various ways depending on factors such as formulation method, administration mode, age, weight, sex, pathological condition, food, administration time, administration route, excretion rate, and response sensitivity of the patient, and those skilled in the art will appreciate these factors The dosage can be appropriately adjusted taking these factors into account. The number of administration may be once a day or twice or more within the range of clinically acceptable side effects, and may be administered to one or two or more sites for the administration site, and total daily or at intervals of 2 to 5 days The number of days of administration may range from 1 to 30 days per treatment. If necessary, the same treatment can be repeated after a titration period. For animals other than humans, the dose is the same as that of a human per kg, or the above dose is converted, for example, by the volume ratio (for example, average value) of the target animal and the organ (heart, etc.) of the human One dose can be administered.

Another aspect provides the use of the fermented product for use in preparing a composition for improving skin conditions.

Another aspect provides the use of the fermented product for use in the manufacture of a medicament for preventing or treating a skin inflammatory disease.

Details regarding the improvement of the skin condition, the composition, the fermented product, and the skin inflammatory disease are as described above.

According to an aspect, a composition comprising a mixed fermented product of young tangja, young green tea and bitter orange flower prevents, improves, or improves skin-related conditions such as skin aging, skin wrinkle improvement, skin elasticity improvement, skin inflammation improvement, skin soothing, or It can be usefully used for treatment.

1 is a result of analyzing the production rate of procollagen in human skin fibroblasts.
2 is a NO assay result confirming the inhibitory effect of nitric oxide production in macrophages.
3 is an ELISA result confirming the level of TNF-α in macrophages.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

Examples 1-2. Preparation of fermented young taengja, young green tea and bitter orange flower

A complex fermentation broth was prepared by fermenting young taengja, young green tea and bitter orange flowers with microorganisms. A specific manufacturing method is as follows.

First, young taengja extract, young green tea extract and bitter orange flower extract were added to the prepared medium and sterilized at 121°C for 15 minutes. For each extract, hot water extract was used. The medium is preferably selected appropriately according to the type of microorganism to be inoculated. For example, a medium prepared from one or a mixture of two or more selected from glucose, yeast extract, malt extract, peptone, glutamic acid, K ₂ HPO ₄ , KH ₂ PO ₄ , NaCl may be used. Young taengja, young green tea, and bitter orange flower added to the medium may be added in an amount of 0.1% (v/v) to 10% (v/v), respectively, but in this embodiment, 3% (v/v) of young taengja extract ), young green tea extract 1% (v/v) and bitter orange flower extract 1% (v/v) were added. Next, after inoculating the microorganisms in the sterilized medium, fermentation was carried out for 72 hours while stirring at 150rpm at 30 to 40 ℃ condition. Next, a step of filtering and obtaining the fermented product was performed. After completion of fermentation, the cells and fermentation residues were separated by centrifugation after completion of fermentation, and a mixed fermented product of young tangja, young green tea and bitter orange flower was prepared through 0.45 μm filtration.

The composition of the medium used in Examples 1 and 2, the amount of extract added, and the type of microorganism are shown in Table 1 below.

Content (%(v/v)) ingredient Example 1 Example 2 glucose 2 2 peptone One 0.5 yeast extract 4 0.5 glutamic acid - 0.5 K ₂ HPO ₄ - 0.1 KH ₂ PO ₄ - 0.05 NaCl - - young tassel extract 3 3 young green tea extract One One Bitter Orange Flower Extract One One water To 100 To 100 microbe genus Lactobacillus
( Lactobacillus sp. ) bacillus genus
( Bacillus sp. )

Examples 3-4 and Comparative Examples 1-4. Preparation of a composition comprising a fermented broth or extract

A composition was prepared with the composition and content (% (v/v)) shown in Table 2 below.

ingredient Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 3 Example 4 division water Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Up to 100 Distilled water butylene glycol 10 10 10 10 10 10 moisturizer 1,2-Hexanediol 2 2 2 2 2 2 antiseptic young tassel extract 3 3 young green tea extract One One Bitter Orange Flower Extract One One Fermentation broth of Example 1 10 Fermentation broth of Example 2 10

In Example 3, 3% (v/v) of young taengja hot water extract, 1% (v/v) of young green tea hot water extract and 1% (v/v) of bitter orange flower hot water extract were added to the medium, and the microorganisms of the genus Lactobacillus were added to the medium. It contains the fermentation broth of Example 1 obtained by fermentation by 10% (v/v).

In Example 4, 3% (v/v) of young taengja hot water extract, 1% (v/v) of young green tea hot water extract and 1% (v/v) of bitter orange flower hot water extract were added to the medium, and The fermentation broth of Example 2 obtained by fermentation was included at 10% (v/v).

Comparative Example 1 contained 3% (v/v) of the hot water extract of Young taengjae.

Comparative Example 2 contained 1% (v/v) of young green tea hot water extract.

Comparative Example 3 contains 1% (v/v) of bitter orange flower hot water extract.

Comparative Example 4 contained 3% (v/v) hot water extract of Young taengjae, 1% (v/v) hot water extract of young green tea, and 1% (v/v) hot water extract of bitter orange flower.

That is, in the case of Examples 3 and 4, since the fermentation broth containing the total of three extracts 5% (v/v) is included in 10% (v/v) of the total composition, the total of the three extracts is 5% (v/v) ) compared to the composition of Comparative Example 4 containing the amount of the active ingredient is reduced to 1/10 level.

Experimental Example 1. Confirmation of skin wrinkle improvement, skin elasticity enhancement and skin aging prevention effect

In order to confirm the skin wrinkle improvement and skin elasticity enhancement effects of the mixed fermented fermented young taengja, young green tea and bitter orange flowers, procollagen production analysis was performed.

Specifically, the compositions of Comparative Examples 1 to 4, Examples 3 and 4 were treated in human dermal fibroblasts (Human Dermal Fibroblast), and the relative production rate of procollagen was confirmed. The treatment concentration of the composition in each experimental group can be selected in the range of 0.1 to 5.0% (v/v), but in this experiment, 1% (v/v) was used. A group not treated with the composition (MO: media only) was used as a negative control group, and a group treated with TGF-β was used as a positive control group.

1 is a result of analyzing the production rate of procollagen in human skin fibroblasts.

As a result, as shown in FIG. 1 , Comparative Examples 1 to 3 containing extracts of each extract of Young Taengja, young green tea, and bitter orange flower, and Comparative Example 4 containing all of Young Taengja extract, young green tea extract, and bitter orange flower extract had little effect on procollagen synthesis. On the other hand, it was confirmed that Examples 3 and 4 containing the mixed fermented young taengja, young green tea and bitter orange flower increased the synthesis of procollagen compared to the negative control group. Examples 3 and 4 showed superior efficacy despite the fact that the amount of the active ingredient was at 1/10 level compared to Comparative Example 4, and thus, it can be seen that an excellent effect was exhibited with a smaller content. In particular, Example 3 including a fermented product using a microorganism of the genus Lactobacillus had the best effect of increasing procollagen synthesis.

From the above results, it can be confirmed that the mixed fermented product of young taengjae, young green tea and bitter orange flower increases procollagen synthesis, thereby exhibiting a remarkably excellent skin wrinkle improvement effect and skin elasticity enhancement effect, and thus has an anti-aging effect. there is.

Experimental Example 2. Confirmation of anti-inflammatory and skin soothing effect

In order to confirm the anti-inflammatory effect of the mixed fermented fermented young Taengja, young green tea and bitter orange flowers, NO assay (Nitric oxide Reduction Assay) and TNF-α level analysis were performed.

Specifically, macrophages were treated with LPS (liposaccharides), and then the compositions of Comparative Examples 1 to 4 and Examples 3 and 4 were treated. The treatment concentration of the composition in each experimental group can be selected in the range of 0.1 to 5.0% (v/v), but in this experiment, 1% (v/v) was used. The nitric oxide production inhibitory effect was confirmed by NO assay, and the level of pro-inflammatory cytokine TNF-α was confirmed by ELISA (enzyme-linked immunosorbent assay). A group not treated with the composition (MO: media only) was used as a negative control group, and a group treated only with LPS was used as a positive control group.

2 is a NO assay result confirming the inhibitory effect of nitric oxide production in macrophages.

3 is an ELISA result confirming the level of TNF-α in macrophages.

As a result, as shown in FIGS. 2 and 3, Comparative Examples 1 to 3 containing extracts of each extract of young taengjae, young green tea, and bitter orange flower, and young tangja extract, young green tea extract and bitter orange flower extract were all included. Compared to Comparative Example 4, Examples 3 and 4 containing a mixed fermented fermented young taengja, young green tea, and bitter orange flower were excellent in the nitric oxide production inhibitory effect and the TNF-α reduction effect. Examples 3 and 4 showed superior efficacy despite the fact that the amount of the active ingredient was at 1/10 level compared to Comparative Example 4, and thus, it can be seen that an excellent effect was exhibited with a smaller content. In particular, Example 3 including a fermented product using a microorganism of the genus Lactobacillus was the most excellent in the nitric oxide production inhibitory effect and the TNF-α reduction effect.

From the above results, it can be confirmed that the mixed fermented product of young taengjae, young green tea and bitter orange flower has a remarkably excellent anti-inflammatory effect and a skin soothing effect.

In summary, it was found that the mixed fermented product of young tangja, young green tea and bitter orange flower had excellent skin wrinkle improvement effect, skin elasticity enhancement effect, skin aging prevention effect, anti-inflammatory effect and skin soothing effect.

Claims (9)

Contains a mixed fermented product of young tangja, young green tea and bitter orange flowers,
The fermented product is obtained by fermentation by microorganisms of the genus Lactobacillus ( Lactobacillus sp. ) or the genus Bacillus ( Bacillus sp. ),
A cosmetic composition that increases the expression of procollagen. The cosmetic composition according to claim 1, wherein the fermented product is a mixed fermented product of young taengja extract, young green tea extract, and bitter orange flower extract. The cosmetic composition according to claim 1, wherein the fermented product is obtained by fermenting a mixture comprising young taengja extract, young green tea extract, and bitter orange flower extract in a volume ratio of 1-10:1-10:1-10. The cosmetic composition of claim 1, wherein the cosmetic composition is for skin aging prevention, skin wrinkle improvement, or skin elasticity enhancement. Contains a mixed fermented product of young tangja, young green tea and bitter orange flowers,
The fermented product is obtained by fermentation by microorganisms of the genus Lactobacillus ( Lactobacillus sp. ) or the genus Bacillus ( Bacillus sp. ),
Inhibiting nitric oxide production, or inhibiting the expression of TNF-α, a cosmetic composition. The cosmetic composition of claim 5, wherein the cosmetic composition is for improving skin inflammation or soothing the skin. delete Contains a mixed fermented product of young tangja, young green tea and bitter orange flowers,
The fermented product is obtained by fermentation by microorganisms of the genus Lactobacillus ( Lactobacillus sp. ) or the genus Bacillus ( Bacillus sp. ) A pharmaceutical composition for preventing or treating skin inflammatory diseases. Young taengja, young green tea and bitter, comprising the step of incubating a combination of young dandelion, young green tea and bitter orange flowers with microorganisms of the genus Lactobacillus or Bacillus sp. to obtain a fermented product. A method for producing a complex fermented product of orange flowers,
The fermented product increases the expression of procollagen, inhibits nitric oxide production, or inhibits the expression of TNF-α, the method.

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