KR100967018B1 - Cosmetic composition comprising a herbal extration - Google Patents

Abstract

The present invention relates to a skin whitening cosmetic containing a plant extract that is safe for the skin and inhibits tyrosinase activity and has an effect of inhibiting melanin production. The cosmetic containing the plant extract of the present invention is a mallow ( Malva Sylvestris) in a conventional cosmetic composition. ), Extracts from flowers and leaves of Primula Veris , Alchemilla Vulgaris , tail grass , Achillea Millefolium plants, and extracts from leaves of Menthe Herba and Melissa officinalis Yeast extract is characterized in that the composition is mixed at a certain ratio.

As described above, the extract of the present invention is a cosmetic containing each component of the extract is a natural extract and microbial fermentation high skin safety, has a skin color improvement effect at a concentration that does not affect the stability of the product and reduces pigmentation It can be used at effective concentration in skin improvement and whitening cosmetics. .

Tyrosinase, whitening

Description

Skin whitening cosmetics containing plant extracts {Cosmetic composition comprising a herbal extration}

The present invention relates to a skin whitening cosmetic containing a plant extract, and more particularly, to a skin whitening cosmetic containing a plant extract having a melanin production inhibitory effect by being safe for the skin and inhibiting tyrosinase activity.

The production of melanin pigment by ultraviolet rays is a major cause of skin pigmentation, and this melanin pigment is a phenolic polymer made by tyrosinase enzyme, and tyrosinase protects skin damage caused by UV rays evenly distributed to various organisms. Do the skin protection mechanism. Therefore, inhibition of tyrosinase, a major cause of melanogenesis and pigmentation, has become an important issue in the study of skin whitening.

Tyrosinase inhibitors developed to date include ascorbic acid, kojic acid, hydroquinone and peroxides such as hydrogen peroxide, zinc peroxide, and magnesium peroxide. However, these substances were often insufficient in stability and safety. Recently, many derivatives and vegetable whitening agents have been known which have a smaller whitening effect than the above materials but have improved stability and safety. Such vegetable whitening agents are representative examples of yeast acid and yeast acid derivatives disclosed in Japanese Patent Application Laid-Open No. 53-3538, and natural products such as Schizandrae disclosed in Korean Patent Registration No. 228741. However, even in the case of the substance has a disadvantage that the inhibitory activity of tyrosinase is weak.

The present inventors extract active ingredients from various plants that can help whitening, and also by constantly mixing fermented microorganisms effective in whitening, thereby increasing the inhibitory activity of tyrosinase, as well as safety. The present invention has been completed with the focus on maintaining good quality.

Therefore, an object of the present invention is to extract the active ingredient from the various plants exhibiting the above-described tyrosinase inhibitory effect, and also to the skin whitening cosmetics having high inhibitory activity of tyrosinase by constantly mixing fermented microorganisms which are also effective in whitening. It is to provide.

Furthermore, the present invention is to provide a cosmetic containing the above-mentioned extract that does not inhibit the stability of the existing cosmetics while showing a useful effect as a cosmetic on the skin whitening.

Cosmetics containing the plant extract of the present invention for achieving the above object is Malva Sylvestris , Primula Veris , Alchemilla Vulgaris , tail grass , Yarrow ( Achillea Millefolium ) in a conventional cosmetic composition Extracts and extracts from the leaves and peppermint ( Menthe Herba ), leaves of the plant ( Melissa officinalis ) and yeast extract of the plant is characterized in that the composition is mixed.

Each of the components may be in a certain ratio but preferably the same weight.

The extract according to the present invention described above is mallow, primrose, virginia, tail grass, Yarrow plant flower and leaf extract and peppermint, Meredith leaf extract is formed by pulverizing each of these flowers or leaves to powder and water or The organic solvent can be extracted by using each or a mixture thereof as a solvent.

The present invention is characterized in that the extract obtained as described above is added to and mixed with the yeast extract and a conventional cosmetic in a certain ratio to obtain a cosmetic.

In the cosmetic composition of the present invention, more preferably, one or more selected from lower alcohols such as glycerin is mixed at a predetermined ratio.

Yeast extract, another constitution of the present invention, can be obtained from a culture solution.

Furthermore, the formulation of the cosmetic composition containing the extract according to the present invention can be prepared in addition to the usual cosmetic formulations such as skin lotion (skin), lotion, essence, cream.

Extract according to the present invention is characterized in that it is contained in an amount of 0.001 to 50% by weight, preferably 0.01 to 5% by weight relative to the dry weight of the cosmetics attached.

If it is contained in an amount of 0.001% by weight or less, tyrosinase inhibitory activity, which is an action effect of the extract according to the present invention, is hardly exhibited, and when it exceeds 50% by weight, it is not only detrimental to the stability of the cosmetics, but also shows a synergistic effect of the effect of excess addition. Not desirable.

On the other hand, the cosmetic composition according to the present invention may contain other ingredients that can enhance or aid the main effect of the present invention in addition to the above-described extract, of course, for example whitening conventionally as such an auxiliary ingredient Kojic acid, arbutin, ascorbic acid derivatives and the like which are known to have an action.

As described above, the characteristics of each of the compositions constituting the extract according to the present invention are as follows.

Mallow is called Malva Sylvestris , derived from the Greek word "malakos" which means "soft" or "stabilizing", and the areas that are mostly used are pale red or purple flowers between June and September. It is a leaf and a flower. Rich in mucus and tannins, it is known to be effective in relieving bronchitis and cough and gastritis.

Primula Veris is a perennial herbaceous primrose, which has been used for a long time in Europe. It is used in Jinhae, expectorant, relieving, bronchitis in Korea and contains a large amount of saponin.

Peppermint ( Menthe Herba ) is a labiatae and a plant that is effective in analgesic, antiseptic, astringent and circulatory system strengthening. In particular, it has the effect of expanding the capillaries of the skin. The main ingredient is menthol, which contains about 77% to 78%, and there are menthol (menthone), menthol (menthol), camphene (limonene), isomentone (isomenthone) and pinene (pinene). In addition, there are various forms of flavonoids that have been tanned with methylnin and triterpene.

Our Lady of Alchemilla Vulgaris is derived from the Arabic alkemelych, meaning 'alchemy'. St. Mary's has been used to treat gynecological diseases for a long time, and leaf has been used as a cosmetic ingredient. In particular, it is known that calotannin and flavonoid components are present in the leaves.

Kkoripul is widely distributed in Korea in Scrophulariaceae (Scrophulariaceae) perennial plant. In the name of Chinese medicine, it is also called cefolipapa nap (細葉 婆婆 納), and is effective in treating emphysema. Tannins and flavonoids are present in large quantities.

Melissa officinalis is a perennial herb of Lamiaceae and is known in Europe as a herb. Sedation, blood pressure lowering through the nervous system, heart strengthening is known. Essential oils, composed of about 70 terpenes, contain 0.02-0.3%.

Yarrow is a perennial herb from the Asteraceae family and is known in Europe as the scientific name Achillea Millefolium . The scientific name stems from the Iliad hero Achilles' treatment of the wounded soldiers in the Trojan War. Hemostatic action, disinfection, tonic effect is known.

The present invention constituted as described above can be given not only a whitening effect by extracting these active ingredients into the composition of each plant constituting the extract of the present invention containing a large amount of tannin, flavonoid components as described above By constructing the extract at a maintenance rate as a cosmetic composition, it is possible to provide a cosmetic composition excellent in inhibitory activity and safety of tyrosinase.

In addition, the yeast extract of the yeast extract which is a constituent of the present invention is well known in the genetic system, it is known to be advantageous for polypeptide production by simple fermentation conditions than any kind of microorganism. Yeast is used for whitening, moisturizing and skin softening along with Lactococcus and Streptococcus.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited thereto.

Example 1

The flowers and leaves of the mallow, primrose, virginia, tail grass and yarrow were washed with purified water, dried and then powdered. In the case of mint and Meritz, only leaves were powdered. Process of pulverizing the powdered plant mixture with 85% lower alcohol for 1 day and then ultra-high frequency treatment for 2 hours, ultra-high frequency treatment of the supernatant with 85% lower alcohol for 2 hours, and filtering the layer solution Was repeated two more times. Concentrated under reduced pressure at 50 ℃ or less to obtain a powdery plant extract, which was mixed 1: 1 with yeast extract to obtain the extract of the present invention.

Example 2-8

Except for each plant constituting the extract of the present invention was extracted in the same manner as in Example 1 to obtain each extract in the final yield as shown in Table 1 below.                     

Botanical name yield(%) Example 2 Mallow 6.5 Example 3 Primrose 10.2 Example 4 Our Lady 7.9 Example 5 Tail grass 24.4 Example 6 Yarrow 8.4 Example 7 mint 13.4 Example 8 Meritz 16.2

Experimental Example 1 : Tyrosinase activity inhibition experiment

The extracts obtained according to Examples 1 to 8 were diluted in ethanol at each concentration. 5 µl of 1.5 mM L-tyrosine and 40 µl of 25 mM L-DOPA were added to a 96-well microplate, followed by 40 µl of 10, 100, 500, 1000 µl / ml samples. . 80 μl of 67 mM phosphate buffer (pH 6.8) was added to bring the total volume with the sample to 165 μl. 80 μl of buffer was added to the blank, and 80 μl of mushroom tyrosinase (60 U / ml) was added to the remaining sample solution, followed by incubation at 37 ° C. for 30 minutes. The amount of dopa chrome produced using an ELISA reader was measured for absorbance at 490 nm, and the inhibition rate was calculated as follows.

Inhibition rate = [(A490 of the control A490-sample solution added) / control A490] × 100

Kozinic acid was used as a positive control, and from the above method, the tyrosinase activity inhibition rate of the extract obtained according to each example was measured and shown in Table 2 below.                     

Concentration (μl / ml) % Inhibition Example 2 10 2.2 Example 3 10 4.0 Example 4 10 1.0 Example 5 10 1.9 Example 6 10 3.0 Example 7 10 4.9 Example 8 10 3.9 Example 1 10 9.4 Control group (kozinic acid) 10 1.9

As shown in Table 2, Examples 1 to 8 according to the present invention showed very high melanin production inhibitory effect at a low concentration of 10 μl / ml. In addition, it can be seen that in Example 1 which is a mixture rather than the effect of each extract, the whitening effect is excellent.

Experimental Example 2 Cytotoxicity and Inhibition of Intracellular Melanin Production

B16 mouse melanoma cells (mouse melanoma) cells were incubated in DMEM medium conditions containing 10% FBS and inoculated with cell lines at a concentration of 1 × 10 ⁴ cells / 24 wells. After 24 hours of incubation at 37 ° C. in a 5% CO ₂ environment, the extract obtained in Example 1 was treated with 10 μl of 1, 10, 100 μl / ml concentration for 3 days after changing to 1000 μl of fresh medium per well every day. Incubated for more days. The measurement of cell proliferation was calculated after measuring the cell concentration using the dimethylazole-diphenyltetrazolium bromide (MTT) reduction method.

[Equation 1]

         Cell proliferation time of control group

* Cell growth rate = ----------------------- × 100

         Cell growth time of the experimental group                     

Type of sample Cell growth rate (%) Control 100 1 μl / ml 99 ± 0.5 10 μl / ml 99.2 ± 0.4 100 μl / ml 98.9 ± 0.7

As a result of the above experiment, it was found that the composition of the present invention according to Example 1 was not cytotoxic because it did not affect the cell viability.

Preparation Example 1

Preparation Example of the flexible longevity containing the extract according to Example 1 is shown in Table 3.

ingredient Content (% by weight) Salicylic acid 0.5 1,3-butylene glycol 5.0 Monolauric acid polyoxyethylene sorbitan 1.3 Polyoxyethylene lauryl ether 0.7 ethanol 15.0 Spices 0.2 antiseptic Quantity Extract of Example 1 0.05 Purified water Remainder

Production Example 2

Preparation of the lotion containing the extract according to Example 1 is shown in Table 4.                     

ingredient Content (% by weight) Stearic acid 0.5 ethanol 10.0 Vaseline 1.0 Squalane 2.5 Liquid paraffin 6.0 Monooleic acid polyoxyethylene sorbitan 1.5 1,3-butylene glycol 8.0 Ethylenediaminetetraacetic acid sodium 0.05 Triethanol amine 0.8 Spices 0.2 antiseptic Quantity Antioxidant Quantity Extract of Example 1 0.05 Purified water Remainder

Production Example 3

Preparation of the essence containing the extract according to Example 1 is shown in Table 5.

ingredient Content (% by weight) glycerin 5.0 1,3-butylene glycol 5.0 Sesquioleate sorbitan 0.4 Polylauric acid polyoxyethylene sorbitan 1.0 Paraoxybenzoic Acid Methyl 0.2 ethanol 2.0 Purified water Remainder Vaseline 5.0 Cetanol 0.2 Extract of Example 1 0.5

Preparation Example 4

Preparation of the cream containing the extract according to Example 1 is shown in Table 6.                     

ingredient Content (% by weight) Stearic acid 0.5 ethanol 10.0 Vaseline 1.0 Squalane 2.5 Liquid paraffin 6.0 Monooleic acid polyoxyethylene sorbitan 1.5 1,3-butylene glycol 8.0 Ethylenediaminetetraacetic acid sodium 0.05 Triethanol amine 0.8 Spices 0.2 antiseptic Quantity Antioxidant Quantity Extract of Example 1 0.05 Purified water Remainder

Comparative Example 1: Lotion

ingredient Content (% by weight) Stearic acid 0.5 ethanol 10.0 Vaseline 1.0 Squalane 2.5 Liquid paraffin 6.0 Monooleic acid polyoxyethylene sorbitan 1.5 1,3-butylene glycol 8.0 Ethylenediaminetetraacetic acid sodium 0.05 Triethanol amine 0.8 Spices 0.2 antiseptic Quantity Antioxidant Quantity Purified water Remainder

Comparative Example 2: Essence

ingredient Content (% by weight) glycerin 5.0 1,3-butylene glycol 5.0 Sesquioleate sorbitan 0.4 Polylauric acid polyoxyethylene sorbitan 1.0 Paraoxybenzoic Acid Methyl 0.2 ethanol 2.0 Vaseline 5.0 Cetanol 0.2 Purified water Remainder

Comparative Example 3: Cream

ingredient Content (% by weight) Stearic acid 0.5 ethanol 10.0 Vaseline 1.0 Squalane 2.5 Liquid paraffin 6.0 Monooleic acid polyoxyethylene sorbitan 1.5 1,3-butylene glycol 8.0 Ethylenediaminetetraacetic acid sodium 0.05 Triethanol amine 0.8 Spices 0.2 antiseptic Quantity Antioxidant Quantity Purified water Remainder

Experimental Example 3: Skin Safety Test

In order to examine the skin irritation properties of the products prepared according to the conventional methods in Preparation Examples 1 to 4 and Comparative Examples 1 to 3, an appropriate amount (2.0 g) of each sample was applied to the lower arm and hip for 30 healthy adult men and women. Buried in a test hazel chamber for 48 hours, the hazel chamber was removed, and after 30 minutes to 1 hour, the skin condition was visually read and the first plate was determined and then removed. After 48 hours, it was read again as a second judgment. The results are shown in Table 11 below.

Stimulus product Number of test subjects Judgment result Stimulus - ± + ++ +++ Primary Secondary Primary Secondary Primary Secondary Primary Secondary Primary Secondary Preparation Example 1 30 29 30 0.27 Preparation Example 2 30 30 30 0 Preparation Example 3 30 29 30 0.27 Production Example 4 30 30 30 0 Comparative Example 1 30 30 30 0 Comparative Example 2 30 30 30 0 Comparative Example 3 30 29 30 0.27

* Judgment Table

  -: No reaction (Irritation level: 0)

  ± suspected positive reaction. Slight erythema reactions. (Irritation degree: 0.5)

  +: Slight positive reaction. Erythema, papules, positive pullback reaction (irritation degree: 1.0)

  ++: Severe positive reaction. Vesicular reactions: erythema, papules, infiltrating lesions, vesicles (irritation degree: 2.0)

  +++: Severe positive reaction. (Irritation degree: 3.0)

[Equation 2]

         (± responders x 0.5) + (+ responders x 1) + (++ responders x 2) + (+++ responders x 3)

* Stimulus = ---------------------------------------------- ------------ x100x1 / 2

                      Total number of investigators x response rate (3)                     

From the skin irritation test results described above, irritation was hardly recognized in the products of the examples.

Experimental Example 4 Measurement of Melanin Index

After applying the cosmetics prepared according to Preparation Examples 1 to 4, the melanin index was measured by using a melanin-erythema index meter and shown in Table 12.

Melanin index Before use after use Experimental group 110.35 ± 30.41 93.40 ± 31.45 Control 102.47 ± 23.49 111.83 ± 33.03

From the above results, the experimental group using the cosmetics according to Preparation Examples 1 to 4 shows that the melanin index was reduced compared to the control group. Therefore, it can be seen that the cosmetic composition containing the extract of Example 1 according to the present invention has a whitening effect.

Experimental Example 5 : Stability Experiment

In order to test the stability of the cosmetics according to the present invention, the cosmetics prepared according to Preparation Examples 1 to 4 were subjected to stepwise 5 ° C, 37 ° C, 43 ° C, 50 ° C and 50 ° C at -5 ° C to maintain a constant temperature at room temperature. The degree of dissociation and the degree of discoloration were measured by storing in a constant temperature bath for a period of time. The results are shown in Table 13 below. At this time, the degree of product separation and discoloration were evaluated by classifying into the following four grades.

* Standards for product separation and discoloration

0: No change 1: Separated slightly (discoloration)

2: severely separated (discolored) 3: extremely severely separated (discolored)

Temperature Duration (months) Separation and discoloration Preparation Example 1 Production Example 2 Production Example 3 Preparation Example 4 5 ℃ One 0 0 0 0 Room temperature 6 0 0 0 0 37 ℃ 3 0 0 0 0 43 ℃ 2 0 0 0 0 50 ℃ One 0 0 0 0 -5 ℃ ~ 50 ℃ One 0 0 0 0

As can be seen in Table 13, it can be seen that the cosmetics prepared according to Preparation Examples 1 to 4 are not separated and discolored and are stable.

As described above, the cosmetic composition containing the extract of the present invention is not only high in skin safety as each component of the extract is a natural extract and a microbial fermentation, but moreover, the extract according to the present invention has a concentration that does not affect the stability of the product. It has the effect of improving skin color and reduces pigmentation, so it can be used at effective concentration in skin improvement and whitening cosmetics.

Claims (3)

Extracts from flowers and leaves of Malva Sylvestris , Primula Veris , Alchemilla Vulgaris , tail grass , Yarrow ( Achillea Millefolium ) plants using water or organic solvents; Extract extracted from the leaves of Menthe Herba and Melissa officinalis using water or an organic solvent; And Skin whitening cosmetics containing a plant extract, characterized in that it comprises a yeast extract. The method of claim 1, Skin whitening cosmetics containing a plant extract, characterized in that the total content of all the extracts contained in the whitening cosmetics is 0.01 to 5% by weight relative to the dry weight of the whitening cosmetics.  The method according to claim 1 or 2, Skin whitening cosmetics containing a plant extract, characterized in that the formulation of the whitening cosmetic is any one selected from flexible lotion, lotion, essence, cream.