KR101326556B1 - Cosmetic composition for ultraviolet-proof containing the extract of sanguisorba officinalis and v. linariaefolia - Google Patents

Abstract

The present invention relates to a cosmetic composition which has UV protection effects and, more specifically, to a cosmetic composition containing, as an active ingredient, a mixture extract of Sanguisorba officinalis and Veronica linariaefolia with safety to human body and antioxidation and UV protection effects.

Description

Cosmetic composition for sunscreen containing cucumber and tailweed mixed extract {Cosmetic composition for ultraviolet-proof containing the extract of Sanguisorba officinalis and V. linariaefolia}

The present invention relates to a sunscreen cosmetic composition containing a cucumber and tail grass extract, specifically, to a cosmetic composition containing a mixture of cucumber grass and cinnabar paste having an excellent sunscreen activity by the synergistic action of cucumber and tail grass extract as an active ingredient It is about.

Most people respond easily to daylight during growth, but as age increases, skin tissue gradually changes, causing wrinkles caused by sunlight, thickening and sagging of the skin, loss of skin elasticity, roughness and dryness of the skin. Phenomenon, skin stains, etc. will occur. Photo-aging is a term that collectively refers to the result of the accumulation of these effects of sunlight. Such photo-aging of the skin is a phenomenon in which the skin's ability to cope with light weakens wrinkles as the epidermis and the dermal tissue change due to sunlight, and the elasticity gradually decreases as the skin becomes yellowish and thicker.

In particular, with the recent acceleration of the industrial society, the level of culture has been rapidly improved, and the seriousness of indoor and outdoor air pollution due to the increase in the number of automobiles, heating, and air-conditioning has become more and more serious. Air pollution due to environmental factors has increased. Occasionally, the ozone advisory is often issued because of this phenomenon. In addition, in the spring and autumn, contact dermatitis due to the spread of allergens such as pollen is increasing day by day. In addition, water pollution by industrial wastewater, household sewage, etc. is also spreading, and soil pollution is also increasing day by day.

Meanwhile, as the overall level of culture improves, consumers' desire for beauty is also increasing. In other words, as the awareness of the effects of skin photoaging and pollution on the skin is spreading, it is possible to reduce and improve skin wrinkle formation, whitening effect, UV protection effect, and defense against pollutants beyond the moisturizing, nourishment, or skin beauty in cosmetics. There is a demand for a functional product having an activity of, and the development and release of such a functional product is gradually being activated.

The mechanisms by which UV radiation can be harmful to the skin are: That is, keratinocytes in the skin cells secrete cytokines and the like, and oxidative stress is induced to activate various proteins in the cells. These activated enzymes hydrolyze the triglyceride lipids and thus secrete arachidonic acid in the cells. In addition, secreted arachidonic acid is converted to prostaglandin E2 (PGE2) by the action of cyclooxygenase (cyclooxygenase). The arachidonic acid and PGE2 thus formed in cooperation with cytokines cause various inflammations including erythema in the skin and further promote aging of the skin. In addition, connective tissue forming cells of the skin cells are also activated by a process similar to the activation of keratinocytes by irradiation of ultraviolet rays and can be killed as the amount of ultraviolet rays is increased. Moreover, skin aging is also promoted by DNA damage and the like, which may cause skin cancer. In short, since ultraviolet rays cause redness of the skin, immune suppression, wrinkle generation, and skin aging and skin cancer, blocking of ultraviolet rays is very important, and thus, development of functional cosmetics having such ultraviolet blocking activity is further required.

Korean Patent Laid-Open Publication No. 2008-0103283 "Ultraviolet blocking cosmetic composition" discloses a sunscreen cosmetic composition containing a sunscreen component comprising at least one selected from the group consisting of vanilla extract and chicory root extract, Korea Patent No. 0992088 "Cosmetic composition containing a natural plant extract that provides UV-blocking effect" includes "fermented lotus meat (seeds of lotus), Gaza (fruits) and Goyanggang (Alpinia officinarum Hance) ), A natural plant extract providing a sunscreen effect, characterized in that it contains a natural plant extract obtained by ultra-sonic extraction of Abies koreana.

The inventors of the present invention completed the present invention by showing excellent UV-blocking activity in a mixed extract of cucumber and tail grasses while continuing research on a natural material having excellent sunscreen effect while being safe for skin.

Therefore, an object of the present invention is to provide a sunscreen cosmetic composition containing a cucumber and tail grass mixed extract, which is safe for the skin and has excellent UV blocking activity as an active ingredient.

According to the present invention to achieve the above object, there is provided a sunscreen cosmetic composition containing a mixture of cucumber and tail grass.

At this time, the mixture of Cucumber and Cucumber is extracted with at least one solvent selected from the group consisting of water, anhydrous alcohol having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethylene glycol, propylene glycol and butylene glycol, preferably Preferably it is extracted with anhydrous alcohol or hydrous alcohol having 1 to 4 carbon atoms.

Preferably, the mixed extract is extracted by mixing the cucumber grass and the tail grass in a weight ratio of 1 to 4: 4 to 1, more preferably in a ratio of 1: 1. The cucumber and tail grass mixture extract is contained 0.001 ~ 10% in the total weight of the cosmetic composition. The sunscreen cosmetic composition may be prepared in the form of softening cosmetics, nourishing cosmetics, nutrition cream, massage cream, nutrition serum, essence or pack.

Cosmetic composition containing the cucumber and tail grass mixture extract of the present invention is not only safe for the skin but also excellent in the sunscreen effect.

Hereinafter, the present invention will be described in detail.

Cucumber (Sanguisorba officinalis) is a perennial herb belonging to the genus Rosaceae. Root stems grow long sideways, stems grow straight, up to 1.5m tall. The oval leaves are rounded at the end and the bottom with 5-11 leaflets, and run like feathers, and the edges are evenly triangular. Dark red flowers bloom in inflorescences on long flower beds in July to September. Flowers are about 2.5cm long, 4 calyxes, 4 stamens. The four-cornered fruit is a winged aster and ripens in October.

It grows in hills and plains all over the country, especially in wet soils on mountain slopes. It is called watermelon, namul sprouts, thin cucumber, etc., and each province has many names. Cutting the leaves gives a fresh smell of cucumber, so it is called cucumber. Root is known as fat milk, used in hemostasis, detoxification, and is used for the treatment of keratinemia, excessive menstruation, postpartum abdominal pain and frostbite. The main ingredients are sanguisorbienin and tannin. Breeding is done by digging roots in the autumn, washing the soil well, drying it in the sun, and then breeding by giving up. Similar plants in Korea include fine cucumber (S. tenuifolia var. Alba), large cucumber (S. stipulata), wild cucumber (S. hakusanensis), and long cucumber (S. longifolia).

V. linariaefolia is a particularly small plant in the genus Scrophulariaceae. Thin-leaved tail grass, often called tail grass. It grows in the grass of mountains and fields. The height is 40-80cm. Stems stand straight and branches slightly split. The leaves are opposite or displaced, and are lanceolate or string lanceolate, with sharp ends and jagged teeth. Leaves are 4-8cm long, 5-8mm butterfly, hairs bent over veins on the back. No petioles; In July-August, blue-colored purple flowers bloom in gunshot inflorescences. Inflorescences are 10-30cm long, with curved hairs. Po is row-shaped, corolla is 4 prongs, spreads almost horizontally. Stamens are 2, calyx is 4 pieces, blunt end. Fruits are flat, rounded, ripened in September to October. White flowers are called white tail grasses (for. Alba), broad-leaved lobules, or oval-shaped long ovals, called large tail grasses (var. Dilatata). Young leaves are eaten and planted a lot for ornamental purposes. In the private sector, the entire grass is used as a medicine for the treatment of stroke and cystitis. Distributed throughout Korea.

Cucumber and tail grass used in the present invention can be purchased in Korea or Japan. In the present invention, the cucumber extract may be an extract of an outpost, root or leaf, preferably the extract of the root. The herbaceous extract may be an extract of the outpost or leaf, preferably an extract of the leaf. According to one embodiment of the present invention, the roots and leaves of the tail and tail grasses of cucumber plants are washed clean with purified water, and used according to the extraction method or used as it is crushed. In the present invention, the extraction solvent is water, anhydrous or hydrous alcohol of 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethylene glycol, propylene glycol, butylene glycol and mixed solvents thereof (e.g., hydrous alcohol, hydrous glycerin, etc.) Prepared by extraction with one or more solvents selected from two solvent mixtures or two or more solvent mixtures), and preferably extracted with a solvent selected from water, alcohols having 1 to 4 carbon atoms or hydrous alcohols. Examples of the alcohol having 1 to 4 carbon atoms include methanol, ethanol, n-propanol, iso-propanol, n-butanol and tert-butanol. Of these extractants, hydrous ethanol is preferred, of which 70% hydrous ethanol is most preferred.

Cucumber and tail grass extract of the present invention can be extracted by the following method. According to one embodiment of the present invention, after crushing the dried cucumber and tail grasses, the extraction solvent selected from 1 to 50 times the volume of water, C 1-4 anhydrous alcohol or hydrous alcohol is added to 3 at 4 ~ 30 ℃ After immersion for 20 days to extract the active ingredient, the extraction solvent is obtained by concentrating with a vacuum condenser. According to another embodiment of the present invention, the cucumber and tail grass dry matters are pulverized, using one or more solvents selected from the group consisting of 1 to 30 times the volume of water, anhydrous alcohols having 1 to 4 carbon atoms or hydrous alcohol. Then, in order to prevent the solvent from evaporating, an extract having a cooling condenser is heated and extracted for 1 to 48 hours at 30 to 100 ° C. or deposited for 1 to 10 days at 5 to 30 ° C. to extract the active ingredient. Obtained by concentration under reduced pressure concentrator. Moreover, it can manufacture by using together the well-known ultrasonic extraction method etc. to the extraction solvent enumerated above.

Cucumber and tail grass extract of the present invention is more preferably extracted with water, anhydrous or hydrous alcohol of 1 to 4 carbon atoms, the extraction temperature is preferably 50 ~ 100 ℃. In the present invention, the mixing ratio of the cucumber grass and the tail grass is 1-4: 4-1, and more preferably, having a mixing ratio of 1: 1 shows excellent properties in yield and UV blocking effect. Cucumber and tail grass mixture extract in the present invention may be prepared by mixing after extracting cucumber grass and tail grass, and may also be prepared by mixing after each extract of cucumber grass and tail grass. Cucumber and tail grass mixture extract of the present invention prepared as described above is excellent in antioxidant and UV blocking effect, UV-containing cosmetic composition for blocking was excellent formulation stability.

Cucumber and tailweed extract of the present invention may be used in the form of a powder dried by extracting the extract extracted with the extraction solvent under reduced pressure, concentrated freeze-dried powder of the concentrate or spray dried concentrate. Cucumber and tail grass mixture extract in the sunscreen cosmetic composition of the present invention may be added in an amount of 0.001% to 10% by weight relative to the total weight of the cosmetic composition in powder form or liquid form, preferably to the total weight of the cosmetic composition It is added in an amount of 0.01 to 5% by weight in powder form or liquid form.

The cosmetic composition of the present invention is not particularly limited in its formulation. For example, it may be formulated such as lotion, nutritional lotion, nutritional cream, massage cream, essence, pack, emulsion type foundation, solid type foundation, Lt; / RTI > It may also be a form of bath cleanser, shampoo, soap, and the like.

In addition, in the cosmetic composition of each formulation, other components other than the above-mentioned cucumber and tail grass extract may be arbitrarily selected and blended according to the formulation or purpose of use of other cosmetics. For example, a coloring agent (pigment), a flavoring agent (flavor), a suspending agent, an emulsifier, a dissolution aid, a stabilizer, an isotonic agent, a pH adjuster, a viscosity adjuster, a solvent and the like can be included. The cosmetic composition of the present invention may include at least one other active ingredient exhibiting a self-sunscreen effect in addition to the cucumber and tail grass extract.

[Example]

Hereinafter, the configuration and effects of the present invention will be described in detail with reference to Examples and Test Examples, but the present invention is not limited thereto.

Example  1: Preparation of Cucumber Extract Powder

After washing the roots of cucumbers purchased in Korea with purified water, add 1 kg of distilled water to 50 g of dried cucumbers, and extract them by boiling at 80 ℃ ~ 100 ℃ for 2 hours in an extractor equipped with a cooling condenser. Thereafter, the filtrate was filtered through a 300 mesh filter paper, and the precipitate was left to stand at room temperature for 1 week, and the precipitate was filtered twice with an Edbentech No. 5 filter paper and a Whatman GFC 150 mm filter paper. After concentrating at a temperature of 40 ° C. to 50 ° C. using a vacuum concentrator, 10 g of the titled extract powder was dried using a spray dryer (model name: B ?? 290, manufactured by BUCHI) under the following conditions.

-Inlet temperature: 180 ℃

-Aspirator efficiency: 100% (35cm3 / hr)

-Pump efficiency: 25% (7.5mL / min)

-Nozzle Cleaner 4

- Rotameter: 30 mm (357 Liter / hr)

Examples 2-5: Preparation of Cucumber Extract Powder According to Extraction Solvent

Cucumber extract powder was prepared in the same manner as in Example 1 by using different extraction solvents. Extraction solvents and the results are shown in Table 1 below.

division Extraction solvent Powder (g) Example 2 30% ethanol 10.0 Example 3 50% ethanol 10.5 Example 4 70% ethanol 11.0 Example 5 100% ethanol 9.5

Example  6: Tail  Preparation of Extract Powder

After washing the tail grass leaf purchased in Korea with purified water, 1 kg of distilled water was added to 50 g of dried tail grass leaf and extracted by boiling at 80 ° C. to 100 ° C. for 2 hours in an extractor equipped with a cooling condenser. do). Thereafter, the mixture was filtered through a 300-mesh filter paper, left at room temperature for 1 week, and the precipitate was filtered twice with a filter paper of Edbentek No. 5 and a Wattman GFC 150 mm filter. After concentrating at a temperature of 40 to 50 ° C. using a vacuum concentrator, 10 g of the title extract powder was dried using a spray dryer (model name: B ?? 290, manufactured by BUCHI) under the following conditions.

-Inlet temperature: 180 ℃

-Aspirator efficiency: 100% (35cm3 / hr)

-Pump efficiency: 25% (7.5mL / min)

-Nozzle Cleaner 4

- Rotameter: 30 mm (357 Liter / hr)

Examples 7 to 10 Preparation of the tail grass extract powder according to the extraction solvent

Different extract solvents were prepared in the same method as in Example 6 tailwood extract powder. Extraction solvents and the results are shown in Table 2 below.

division Extraction solvent Powder (g) Example 7 30% ethanol 10.0 Example 8 50% ethanol 10.5 Example 9 70% ethanol 10.5 Example 10 100% ethanol 9.5

Examples 11 to 17: powder preparation of cucumber and tail grass extract according to the mixing ratio

The powder ratio of Example 11 to 17 was prepared in the same manner as in Example 6, using the same extraction solvent as Example 9 by adjusting the weight ratio of cucumber and tail grass as shown in Table 3.

division Mixing ratio
(Cucumber: tail grass) Yield (%) Example 11 1: 1 (25.0 g: 25.0 g) 15 Example 12 2: 1 (33.3 g: 16.7 g) 12 Example 13 3: 1 (37.5 g: 12.5 g) 11 Example 14 4: 1 (40.0g: 10.0g) 11 Example 15 1: 2 (16.7 g: 33.3 g) 12 Example 16 1: 3 (12.5 g: 37.5 g) 12 Example 17 1: 4 (10.0g: 40.0g) 10

Examples 18 to 29: powder preparation of cucumber and tailweed extract according to the extraction solvent

Extracted in the same manner as in Example 11, except that 25.0 g of dried cucumber grass and 25.0 g of tail grass (used in the same weight ratio as in Example 11) were used and washed with purified water, and 2 kg of the extraction solvent described in Table 4 below was used. The title powders of Examples 18-29 were prepared. The results are shown in Table 4 below.

division Extraction solvent Yield (g) Example 18 30% functional ethanol 8g Example 19 50% functional ethanol 8.5 g Example 20 70% functional ethanol 13g Example 21 30% functional ethanol 9g Example 22 50% functional ethanol 10g Example 23 70% functional ethanol 12.5 g Example 24 30% water butylene glycol 7g Example 25 50% water butylene glycol 8g Example 26 30% water propylene glycol 8g Example 27 50% water propylene glycol 7g Example 28 30% water glycerine 6g Example 29 50% water glycerine 7g

As confirmed in the above tables, the yield of the mixed extract was higher than that of the extract of cucumber and caudal grass alone, and especially when mixed with a weight ratio of 1: 1 and extracted with 70% hydrous ethanol, it showed the best efficiency.

Formulation Example 1 Preparation of Cosmetic Water Containing Cucumber and Tailweed Extract

1 kg of the lotion (skin lotion) containing the cucumber extract and the tail extract mixture extract (Example 11) was prepared using the following preparation method in the following Table 5.

number Raw material Content (% by weight) One Cucumber and Cinderella Extract Powder (Example 11) 1.0 2  glycerin 3.0 3  Butylene glycol 2.0 4  Propylene glycol 2.0 5  Polyoxyethylene hardened castor oil 1.0 6  ethanol 10.0 7  Triethanolamine 0.1 8  Phenoxyethanol a very small amount 9  Pigment a very small amount 10  Spices a very small amount 11  Purified water Balance

In Table 5, materials 2, 3, 4, and 8 were added to the reaction container in order, and dissolved by stirring. Subsequently, materials 5 were heated to about 60 ° C. to dissolve. Subsequently, the substance 10 was added to the substance 11 after dissolution. Finally, 1, 6, 7, and 9 were added to the material and sufficiently stirred, followed by aging at 25 ° C. for 3 days to prepare the title lotion.

Formulation Example 2: Preparation of Nutritional Lotion Containing Cucumber and Tailweed Extract

1 kg of the hydrosome nutrient lotion containing the cucumber extract and the tail extract mixture extract (Example 11) was prepared using the following preparation method.

number Raw material Content (% by weight) One Cucumber and Cinderella Extract Powder (Example 11) 1.0 2  Wax 1.0 3  Polysorbate 60 1.5 4  Sorbitan sesquioleate 0.5 5  Liquid paraffin 10.0 6  Sorbitan stearate 1.0 7  Pro-type glycerin monostearate 0.5 8  Stearic acid 1.5 9 Glyceryl Stearate / Pig ?? 400 Stearate 1.0 10 Propylene glycol 3.0 11 Carboxy polymer 0.1 12 Triethanolamine 0.2 13 Phenoxyethanol a very small amount 14 Pigment a very small amount 15 Spices a very small amount 16  Purified water Balance

In Table 6, 10, 11, 13, and 16 materials were mixed and stirred and heated to 80 to 85 ° C., and then put into the manufacturing unit, followed by actuating an emulsifier. Material 12 was dissolved by heating to 80 ~ 85 ℃. After emulsification, cool down to 50 ℃ while stirring using a stirrer, add material 15, cool to 45 ℃, add material 14, add material 1 at 35 ℃, cool to 25 ℃, and 25 ℃. Aged 3 days in to prepare the title nutrition lotion.

Formulation Example 3: Preparation of Nutritional Serum Containing Cucumber and Tailweed Extract

1 kg of hydrosome nutrient serum containing cucumber and tailweed mixed extract powders (Example 11) was prepared using the following preparation method.

number Raw material Content (% by weight) One Cucumber and Cinderella Extract Powder (Example 11) 1.0 2 Xanthan gum 0.02 3 Butylene glycol 10.0 4 Phospholipid 10.0 5 Hydroxyethyl cellulose 0.1 6 Sodium hyaluronate 5.0 7 Phenoxyethanol a very small amount 8 Pigment a very small amount 9 Spices a very small amount 10 Purified water Balance

In Table 7, the materials 2, 3, and 5 were mixed and stirred and heated to 40 to 45 ° C., and then introduced into the production unit, followed by the operation of an emulsifier. After emulsification, the mixture was cooled to 35 ° C. while stirring using a stirrer, and 1 substance was added thereto, cooled to 25 ° C., and aged at 25 ° C. for 3 days to prepare the title nutrition serum.

Formulation Example 4 Preparation of Essence Containing Cucumber and Tailweed Extract

1 kg of a hydrosome essence containing a mixture of cucumber and tail grass extract extracts (Example 11) was prepared using the following preparation method.

number        Raw material Content (% by weight) One Cucumber and Cinderella Extract Powder (Example 11) 1.0 2 Sitosterol 1.7 3  Polyglyceryl 2 Olate 1.5 4  Ceramide 0.7 5 Steares 1.2 6 cholesterol 1.5 7 Dicetyl phosphate 0.4 8 glycerin 5.0 9 Macadamia oil 15.0 10 Carboxyvinyl polymer 0.2 11  Xanthan gum 0.2 12  Phenoxyethanol a very small amount 13  Spices a very small amount 14  Purified water Balance

In Table 8, materials 2, 3, 4, 5, and 6 were homogenized at a constant temperature to prepare nonionic amphiphilic lipids. After mixing the nonionic amphiphilic lipids with materials 1, 7, 8 and 14 and homogenizing it at a constant temperature, it is passed through a microfluidizer, and then the material is heated to 50 to 60 ° C and slowly added to homogenize it. The microfluidizer was passed again. Thereafter, materials 10, 11, 12, and 13 were added to disperse, stabilize, and ripen to prepare the title essence.

Test Example 1 Check the Antioxidant Effect

Nitro Blue Tetrazolium (NBT) reduction method was used to measure the scavenging activity of superoxide radicals produced by Xanthine oxidase. After mixing 1 ml of 0.1 M potassium phosphate buffer (pH 7.5) containing 0.4 mM xanthine and 0.24 mM NBT and 0.6 ml of 0.1 M potassium phosphate buffer (pH 7.5) and 0.1 ml of the sample, xanthine oxidase (0.05 U / ml ) And the mixture is reacted at 37 ° C for 20 minutes. After the reaction was terminated by adding 1 ml of 1 M HCl, the absorbance at 520 nm was measured.

NBT reduction inhibition was calculated by the following formula, the results are shown in Table 9 below.

B (Blank): Absorbance of the reaction solution containing only 0.1M potassium phosphate buffer (pH7.5) containing 0.4mM xanthine and 1M HCl

C (Control): Absorbance of the reaction solution in which 0.24 mM NBT was added instead of the sample.

S (Sample): absorbance of the reaction solution added sample

Name of sample Treatment concentration (wt / v%) Antioxidative effect(%) Cucumber and Cinderella Extract (Example 11) 0.1 96 Cucumber Extract (Example 1) 0.1 91 Tailweed Extract (Example 6) 0.1 91.6 Retinol (SIGMA, USA) 0.1 92 Butylated hydroxyanisole (DUKSAN KOREA) 0.1 90

As shown in Table 9, all of the samples tested at a concentration of 0.1 (wt / v)% was able to confirm the excellent antioxidant effect. In particular, Cucumber and Cucumber mixture extracts showed superior antioxidant effects as compared to Cucumber or Cucumber extract alone, and had better antioxidant effects than retinol and BHT.

Test Example 2 Experiment for Measuring Antioxidant Effect Using DPPH Method

In order to determine the antioxidant effects of the cucumber and tail grass extract obtained in Example 11, antioxidant activity was measured using a DPPH method using a comparative sample of antioxidants such as green tea extract and vitamin E under laboratory conditions.

The DPPH method measures the antioxidant activity by reducing power by using a free group called 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH). In comparison, the free radical scavenging rate is measured at a wavelength of 560 nm. The reagent used was 0.1Mm solution of 2,2-diphenyl-1-picrylhydrazyl free radical (Aldrich Chem. Co., MW = 394.32g / mol) as 61.88mg dissolved in methanol to make 100ml. .

① Add 0.15ml of the sample solution dissolved in 0.15ml of 0.1mM DPPH solution in purified water at 0.01 (wt / v)% concentration to the 96-well plate and stir rapidly. Incubate for 10 minutes at 25 ℃.

② Measure the absorbance St at 560 nm thereafter.

③ In the blank test, measure the absorbance Bt by using distilled water instead of the sample solution as above.

④ Again, the flank of the sample solution was measured in the same manner using methanol instead of the 0.1 mM DPPH solution to measure the absorbance Bo. It was calculated by the following formula, and the results are shown in Table 10.

% Inhibition = [1- (St-So) / (Bt-Bo)] × 100

St: absorbance at 560 nm after free radical scavenging of the sample solution

Bt: absorbance at 560 nm after free radical scavenging of the blank test solution

So: the absorbance at 560 nm before the reaction in the absence of free radicals in the sample solution

Bo: absorbance at 560 nm before the reaction in the absence of the free radical of the blank test solution

Name of sample Treatment concentration (%) Antioxidative effect(%) Cucumber and Cinderella Extract (Example 11) 0.01 88 Cucumber Extract (Example 1) 0.01 60 Tailweed Extract (Example 6) 0.01 45 Vitamin E (SIGMA, USA) 0.01 40

Cucumber and tail grass extract as shown in Table 10 had an excellent antioxidant effect than the extract of cucumber grass, tail grass extract, vitamin E at the same concentration at 0.01% concentration.

Test Example 3: Confirmation of formulation stability

For formulations prepared in Examples 1 to 4, including Cucumber and Cucurbita japonica extracts in opaque glass containers in indoors, refrigerators, and incubators, which were kept constant at room temperature (25 ° C.), refrigerated (4 ° C.) and constant temperature (50 ° C.). It was stored and observed for 12 weeks (color change, discoloration and separation), and the stability was confirmed.

Temperature condition Check stability (discoloration, odor and separation) Formulation Example 1 Formulation Example 2 Formulation Example 3 Formulation Example 4 Indoor (25 ℃) 0 0 0 0 Refrigerated (4 ℃) 0 0 0 0 Constant temperature (50 ℃) 0 0 0 0

<Formulation stability level>

0: No change, 1: Minute change, 2: Change, 3: Extreme change

As shown in Table 11, all of Formulation Examples 1 to 4 were stable, without discoloration, odor and separation phenomenon under 25 ℃, 4 ℃ and 50 ℃ temperature conditions.

Test Example 4 UV protection effect experiment

All samples were diluted using ethanol as a solvent, and the absorbance for each wavelength was measured using a UV-Visible Spectrophotometer (CARY 100 Conc). The concentration of the sample was diluted to about 1000 ppm, and the UV-blocking effect of the composition of the present invention was tested by measuring the absorption ability of each composition in the wavelength range of 200 to 400 nm.

Cucumber extract prepared in Example 1 and the tail grass extract prepared in Example 6 was mixed in a weight ratio of 1: 1 and the result of measuring the UV protection effect of the composition diluted to 100 ppm with ethanol is shown in Table 12 and same. As shown in Table 12, the absorption ratio of the mixed composition of the Cucumber extract and the tailweed extract is 0.716 in the 350 nm region, 1.41 in the 203 nm region, and the like. Although similar, the absorption in the 203 nm region was increased. Therefore, it can be seen that when the tail grass extract is mixed with the cucumber grass extract, the blocking effect against UVA is enhanced. In addition, the mixed composition remained similar to the UVB blocking function. Therefore, it can be seen that the mixed composition of the cucumber and cinnabar extract synergistically improves the overall sunscreen function than when used alone.

Wavelength (nm) 203 250 300 350 Absorption rate 1.41 0.82 0.771 0.716

Experimental Example 5: UV-blocking effect experiment of cosmetics containing cucumber and tail grass mixed extract

The sunscreen effect on the cosmetic formulation of Formulation Example 4 containing a cucumber and tail grass mixed extract was confirmed. Comparative Formulation Example 1 was prepared by containing only the Cucumber extract, Comparative Formulation Example 2 was prepared only by the tailweed extract, and Comparative Formulation Example 3 was prepared by comparing the cosmetics that do not contain both.

The experiment of the sunscreen effect was performed by the in vitro method and the instrument used for the measurement was SPF 290 (Optometrics Corporation, UK).

Test Items Formulation Example 4 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 SPF value 25.5 23.7 24.4 15.3 UVA / UVB Ratio 0.38 0.48 0.41 0.55

As confirmed in Table 13, it can be seen that the cosmetic containing a mixture of cucumber and tail grass extract exhibits excellent UV protection effect compared to cosmetics containing only a cucumber or tail grass extract, or not at all. This indicates that the extract of cucumber and tail grass synergistically increased the UV blocking effect.

Claims (5)

Cosmetic composition for sunscreen containing cucumber and tail grass mixed extract. The method of claim 1, wherein the mixture of cucumber and tailweed is at least one solvent selected from the group consisting of water, anhydrous alcohols having 1 to 4 carbon atoms, ethyl acetate, acetone, glycerin, ethylene glycol, propylene glycol and butylene glycol Cosmetic composition for sun protection, characterized in that to be extracted. According to claim 1, The mixed extract is a cosmetic composition for UV protection, characterized in that the extract is mixed in the weight ratio of cucumber grass and tail grass 1-4: 4-1. According to claim 1, wherein the mixture of cucumber and tail grass extract UV protection cosmetic composition, characterized in that it contains 0.001 ~ 10% in the total weight of the cosmetic composition. According to claim 1, wherein the sunscreen cosmetic composition is a sunscreen cosmetic composition, characterized in that it is prepared in the form of softening water, nourishing cosmetics, nutrition cream, massage cream, nutrition serum, essence or pack.