KR20200009195A - A cosmetic composition comprising ligustrum lucidum extract - Google Patents

Abstract

The present invention provides a cosmetic composition comprising a Ligustrum lucidum extract as an active ingredient. The cosmetic composition is excellent in antioxidant, anti-inflammatory and anti-wrinkle effects.

Description

A cosmetic composition containing the extract of Jeju Orchard Tree as an active ingredient {A COSMETIC COMPOSITION COMPRISING LIGUSTRUM LUCIDUM EXTRACT}

The present invention relates to a cosmetic composition containing the extract jeju tree as an active ingredient.

Skin is an important organ that protects the body from physical, chemical or biological stimuli. In addition, the skin plays an important role in maintaining the homeostasis of the living body, such as controlling the moisture and body temperature inside the skin.

However, as the skin ages, the skin changes due to various internal and external factors. When the secretion of various hormones that regulate metabolism inside the skin is reduced, and the function of immune cells and the activity of skin cells is reduced, various problems such as inflammation and atopy occur in the skin. In addition, free radicals and active harmful oxygen increase due to deterioration of the skin, and melanin is deposited on the cells, and the skin is damaged by blemishes, freckles, wrinkles, and the like.

In order to improve such a skin, various cosmetic external preparations have been developed. In particular, the functional cosmetic composition includes an antioxidant that removes free radicals and free radicals that are the cause of oxidative stress. In general, the antioxidant of cosmetics has the ability to significantly reduce or prevent the phenomenon that the skin function and structure damaged and burned out by oxidative action is significantly reduced, and the purpose is to restore the skin damaged by UV exposure.

The cosmetics industry is developing a number of products using natural products to reduce skin irritation caused by various chemicals. Natural ingredients have less side effects on the skin, and as the consumer's response to cosmetics using natural ingredients has recently increased, the development value of cosmetic ingredients has gradually increased. In particular, medicinal plants are attracting attention as raw materials of cosmetics because they contain a variety of functionalities, including anti-cancer effect.

However, cosmetic products containing extracts derived from natural methods obtained by conventional methods are not fully implemented such as antioxidant, anti-inflammatory and whitening, there was a problem that the skin improvement activity is not maintained continuously. Therefore, the necessity of developing a natural product-derived cosmetic product with excellent functionality and excellent skin stability and skin improvement is steadily emerging.

On the other hand, about 45 species of Ligustrum plants are distributed all over the world, ranging from Asia and the northwestern coast from Europe and Southeast Asia to the continent of Australia. Mainly distributed in East Asia, there are about 29 species in China, one subspecies, nine varieties, and one variant. The drug used in this genus is six species, two variants are also used for medicinal purposes. It is mainly distributed in southern China, Shaanxi and Gansu.

In particular, among the campfire plants are Jeju trees, which are mineral trees distributed in Jeju Island. The fruit of Jeju tree is known to have the function of enhancing immunity, antioxidant, anti-aging, liver protection, anti-tumor.

Therefore, in a situation in which the necessity of developing a natural product-derived cosmetic product having excellent bio stability and skin improvement effect along with excellent functionality is steadily emerging, the present inventors have tried to develop an excellent functional cosmetic composition using Jeju tree.

The present invention is to solve the above-mentioned problems of the prior art, an object of the present invention is to provide a cosmetic composition excellent in functionality comprising the extract jeju tree.

According to one aspect of the invention, there is provided a cosmetic composition comprising the extract jeju tree as an active ingredient.

In one embodiment, the cosmetic composition may further include one or more selected from the group consisting of gyoyoeum extract, pear fennel extract, baekwook extract, buckwheat extract, gobbi fern extract, tansy extract and turmeric extract.

In one embodiment, the cosmetic composition may be for antioxidant and anti-inflammatory.

In one embodiment, the cosmetic composition may be for wrinkle improvement.

In one embodiment, the extract of the cosmetic composition is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3 It can be extracted with one or more solvents selected from the group consisting of butylene glycol.

In one embodiment, the cosmetic composition is supple cosmetics, nourishing cosmetics, moisture cream, nutrition cream, massage cream, nutrition lotion, essence, ampoule, gel, eye cream, oil, foundation, cleansing cream, cleansing foam, cleansing water, It may be formulated into one or more selected from the group consisting of shampoos, rinses, packs, sprays and powders.

Cosmetic composition according to an aspect of the present invention by containing the extract jeju tree, it is excellent in antioxidant, anti-inflammatory and anti-wrinkle effect.

It is to be understood that the effects of the present invention are not limited to the above effects, and include all effects deduced from the configuration of the invention described in the detailed description or claims of the present invention.

1 is a graph evaluating the antioxidant capacity of the composition prepared according to an embodiment of the present invention by the DPPH method.
Figure 2 is a graph evaluating the antioxidant capacity of the composition prepared according to an embodiment of the present invention by the ABTS method.
Figure 3 is a graph showing the wrinkle improvement effect of the composition prepared according to an embodiment of the present invention.
Figure 4 is a graph showing the anti-inflammatory effect of the composition prepared according to an embodiment of the present invention.

The terminology used herein is to select general terms that are currently widely used as possible in consideration of the functions in the present invention, but may vary according to the intention or precedent of the person skilled in the art, the emergence of new technologies and the like. In addition, in certain cases, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the invention. Therefore, the terms used in the present invention should be defined based on the meanings of the terms and the general contents of the present invention, rather than simply the names of the terms.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art. Terms such as those defined in the commonly used dictionaries should be construed as having meanings consistent with the meanings in the context of the related art, and shall not be construed in ideal or excessively formal meanings unless expressly defined in this application. Do not.

The numerical range includes the numerical values defined in the range. All maximum numerical limits given throughout this specification include all lower numerical limits as if the lower numerical limits were clearly written. All minimum numerical limits given throughout this specification include all higher numerical limits as if the higher numerical limits were clearly written. All numerical limitations given throughout this specification will include all better numerical ranges within the broader numerical range, as the narrower numerical limitations are clearly written.

Hereinafter, examples of the present invention will be described in detail, but the present invention is not limited by the following examples.

According to one aspect of the invention, there is provided a cosmetic composition comprising the extract jeju tree as an active ingredient.

The cosmetic composition may include an extract derived from nature as an active ingredient, as well as excellent skin improvement effect and biosafety, and may minimize skin irritation.

The "comprising as an active ingredient" contains an effective amount that can exhibit a skin improving effect, for example, collagen synthesis associated with wrinkle improvement, elasticity or skin whitening, skin irritation, skin soothing effect, etc. Can mean to do.

“ Ligustrum, Jeju Island” lucidum ) ”is also known as dangwang tree and is a kind of wild tree native to Jeju Island. Leaves are large, leathery, broad ovate, broad oval or ovate long oval, acute or obtuse, original or basal, 3-10cm long, 2.5-4.5cm wide, with insignificant residues on the back and flat edges. 5-12mm and reddish brown with leaf veins. Flowers bloom in July-August, flowers are white, abdominal flowers hang at the end of a new branch, 5-12cm in length and width, calyx is flat or wavy at the edge, corolla is 5-6mm long, and tube is slightly longer or equal to lobe. It flips backwards and there are two surgeries. The fruit matures in October, and the nucleus is ovate round, 7-10mm long, ripened in purple, and remains in winter. Branches have fine hairs, dense leaves, egg-shaped oval or slightly circular, short in leaf disease, inflorescences with short hairs on the axial axis.

The cosmetic composition may further include one or more selected from the group consisting of gyoyo lamp extract, pear fennel extract, dolomite extract, buckwheat extract, sesame seed fern extract, cucurbita extract and turmeric extract.

“ Paederia ” scandens ) ”is a vine perennial plant of dicotyledonous plants. The gorilla grows in sunny places at the foot of the mountain or in the grass of the sea. Keilyo lamp is 5 ~ 7m long, with young hairs on it and has a peculiar smell. The gorilla is a species that characterizes the forest edge of the warm temperate evergreen broad-leaved forest, the forest garden, or the rural roadside. If you live in the warm southern regions of the four seasons, part of the ground will stay blue even in winter. Take a long stem and wind up another plant near you to the left. In Korea, where the bitter cold sometimes occurs even in the southern warm zone, it does not rise to the height. Most live low near the surface.

" Agastache " rugosa ) ”is a perennial herb that grows in mountains and fields all over Korea. The growing environment is rich in soil and grows in sunny or half shades. The height is 40-100cm, the leaves are opposite, the length is 5-10cm, the butterfly is 3-7cm, and the tip is pointed and heart-shaped. Flowers bloom in purple, 5-15cm long, 2cm long, and hang like an umbrella at the end of branches and stems. Fruits ripen in October-November. The seeds turn dark brown and contain a lot of seeds in fine form. It is used for ornamental purposes, and young leaves are used for food, and the ground part including flowers is for medicinal purposes.

Hedyotis, supra diffusa ) ”is an annual herb that grows in mountainous wetlands. It is 10-30cm in height. Branches split from the bottom and grow sideways or stand upright. The leaves are opposite each other, have a line shape, no sawtooth, but the edges are clear and only the vein appears. It is 1-3.5 cm in length and 1.5-3 mm in butterfly. Flowers bloom in August-September, white or slightly reddish, 2mm in diameter. Petioles come from axils, 1 ~ 3mm long. The calyx and corolla are divided into 4 pieces each. Corolla fragments are similar in length to tube and have 4 stamens. Fruits are capsules, round, with calyxes remaining at the end of 5mm in diameter.

Houttuynia, supra cordata ) ”is called Eoseongcho and grows in shady forests. Underground stem long, side thin, white. Stems stand straight, 20-50cm high, with several vertical lines, and smell. Leaves are alternate, 3 ~ 8cm long, pointed end, flat edge, chin leaves attached to the bottom of petiole. Plums are capsules, seeds are light brown. Use the plants before flowering as a diuretic and insect repellent, apply them when the leaves are crushed and bitten by boils and poisons. In the private sector, it is used for swelling, purulent and hemorrhoids, and in oriental medicine, plants are used for gonorrhea, enteritis, urinary tract infections, pneumonia and bronchitis. Because the leaves are similar to the leaves of buckwheat and are medicinal plants, they are called weak buckwheat.

Cyrtomium falcatum ) ”is a perennial plant that grows in the crevices of beaches and islands. Root stock is thick and short, stretched sideways, leaves come together at the end. The petiole is 15-40cm long and has scale pieces, especially at the bottom. The scales are egg-shaped or lanceolate, membranous (translucent like thin paper), brown or blackish brown with sharp tips.

In oriental medicine, the root stem and petiole of devil's fern are called total involvement and are used for the antipyretic effect of the epidemic cold, the hemostatic effect of uterine bleeding, tapeworm, and roundworm relief. In the private sector, it was mainly used for hemostasis and roundworm remedies. In recent years, it is widely used as a subsidy along with stone gardens in the garden.

“ Artemisia ” scoparia ) ”is an annual or biennial plant that grows on sandy beaches. The stem is 40-100cm high and grows straight. Has many branches and vertically deep wrinkles. It is usually red in color and initially hairy but gradually disappears. The root leaves and the leaves below the stems wither early. Stem leaves alternate and egg-shaped 2-3 times. Branches are thin like threads. Flowers bloom in August-September, with head-shaped flowers forming conical inflorescences. The flower wrap is spherical and the flower wrap is attached with 2-3 lines. Fruits are achene and ovate. It grows wild in some parts of Korea. It is distributed in Russia, Mongolia, India (India), China, Central Asia, and Europe.

Recalling “ Curcuma longa Rhizoma ”is a perennial plant belonging to the Ginger tree, which is mainly cultivated in tropical and subtropical regions mainly in India, and uses stems and roots for food and medicinal purposes. It is cultivated in India, China, and Southeast Asia. Korea is cultivated in Jeonnam, Jindo, Jeonnam, Haenam, Jeonbuk, Buan, Gyeonggi Siheung, and Chungnam, Chungyang.

The spikes come before the leaves and are enclosed in a broad egg-shaped, pale green cloth. Yellow flowers bloom in the axilla from April to June. The upper paws have a slightly narrow butterfly, the end is pale red, and there are no flowers on the axilla. The outside of the rhizome is light yellow, and the inside is scarlet and smells like camphor. Turmeric uses root stem as a herbal medicine. A spicy, bitter, yellowish medicinal herb that is effective in relieving pain and dysmenorrhea. In India, it is used as a medicine for bruises and sprains, or as a curry powder.

The “extract” may include all materials irrespective of the extraction method or the type of the component, as long as the active ingredient contained in the extract is separated from the natural ingredient by contacting the solvent and the extract raw material under specific conditions. .

For example, extracting a component dissolved in a solvent from a natural product using water or an organic solvent, may include both a specific component of the natural product, such as obtained by extracting only a specific component such as oil. The extract may be powdered after drying, or may be obtained by various conventional extraction methods such as hot water extraction and ethanol extraction.

The extract is selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3-butylene glycol Which may be extracted with one or more solvents, and specifically with ethanol at a concentration of 60 to 80% (v / v).

The active ingredient contained in the raw material may be different in the extraction ratio according to the polarity of the solvent, the ethanol has excellent selectivity in the extraction of physiologically active substances of natural raw materials, the optimal skin improvement effect is realized by the ethanol extraction Can

Since water and ethanol are different in polarity and the active ingredient extracted according to each polarity may be different, the concentration of the ethanol may be appropriately controlled to realize an optimal skin improvement effect. At this time, if the concentration of the ethanol is less than 60% may not be extracted an effective ingredient showing a skin improvement effect, if it is more than 80% may not implement the proper yield.

The extract is washed with water, washed with water, dried and pulverized, and extracted with conventional methods such as reflux circulation extraction, pressure extraction, and ultrasonic extraction for about 1 to 24 hours with a solvent having a volume of 8 to 12 times the weight of the raw material. It can be prepared by filtration. In addition, the extract may be obtained in a powder state by an additional process such as distillation under reduced pressure or freeze drying.

The extract may also include an extract that has undergone conventional purification. For example, the extract may be subjected to various purification methods additionally performed, such as separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity, or affinity). It may comprise a fraction obtained through.

The extract may be extracted for 12 to 18 hours at a temperature of 50 ℃ to 90 ℃ using the ethanol as a solvent.

On the other hand, if the extraction temperature is less than 50 ℃ extraction efficiency is lowered and the active ingredient may not be eluted properly, if it is more than 60 ℃ active ingredient contained in the natural raw material is deformed or lost at a high temperature may reduce the skin improvement effect Therefore, it is preferable to maintain the extraction temperature within an appropriate range. In addition, if the extraction time is less than 12 hours, the active ingredient contained in the natural raw material may not be extracted completely, and if the extraction time is more than 18 hours, the amount of the remaining active ingredient contained in the natural raw material is insufficient, which is inefficient in terms of time and cost. Therefore, it is preferable to appropriately control the extraction time.

The cosmetic composition may be for antioxidant, anti-inflammatory and wrinkle improvement.

The term "antioxidant" refers to the action of inhibiting oxidation, the human body is in the balance between the antioxidant and antioxidant inhibitors, but due to a number of factors to lose this balance state and inclined in the direction of promoting oxidation Oxidative stress may be induced in vivo, resulting in cell damage and pathological diseases.

Reactive oxygen species (ROS), which are a direct cause of oxidative stress, are chemically unstable and highly reactive, making it easy to react with many biomaterials such as DNA, proteins, lipids, and carbohydrates. This can cause irreversible damage to cells and tissues, mutations, cytotoxicity, and cancer, and it can also be a direct cause of aging. By removing or reducing the reactive oxygen species, an antioxidant effect can be obtained to prevent aging and maintain health.

The term "anti-inflammatory" means an inflammation improving effect. The "inflammation" is a mechanism for repairing and repairing the damaged area when an invasion causing a physical action, chemical substance, or bacterial infection to the living body or tissue is applied. Vascularly active substances such as serotonin, bradykinin, prostaglandin, hydroxyeicosatetraenoic acid (HETE), and leukotriene are released, which may lead to inflammation as vascular permeability increases.

The “wrinkle improvement” refers to a phenomenon in which elastic fibers composed of elastin are present together with collagen, and skin elasticity is maintained or improved in a state where the elastin and collagen are sufficiently present. In addition, the "wrinkle improvement" refers to a phenomenon that inhibits or inhibits the formation of wrinkles on the skin or alleviates the wrinkles already generated.

The cosmetic composition is flexible cosmetics, nourishing cosmetics, moisture cream, nutrition cream, massage cream, nutrition lotion, essence, ampoule, gel, eye cream, oil, foundation, cleansing cream, cleansing foam, cleansing water, shampoo, rinse, pack, It may be formulated into one or more selected from the group consisting of spray and powder.

The cosmetic composition may be formulated by conventional methods. Reference may be made to the formulations disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA for the formulation of external skin preparations, and in International Cosmetic Ingredient Dictionary, 6th ed (The cosmetic, Toiletry and Fragrance Association). , Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared in a general emulsion formulation and solubilized formulation. For example, lotion, such as a flexible lotion or a nourishing lotion; Latex, such as facial lotion and body lotion; Creams such as nutrition creams, moisture creams, and eye creams; essence; Cosmetic ointments; spray; Gels; pack; Sunscreen; Makeup base; Foundations such as liquid type, solid type or spray type; powder; Makeup removers such as cleansing cream, cleansing lotion and cleansing oil; Or it may be formulated as a cleaning agent such as cleansing foam, soap, body wash, but is not limited thereto. In addition, the topical skin preparation may be formulated as an ointment, patch, gel, cream or spray, but is not limited thereto.

The cosmetic composition may be appropriately blended with other ingredients within the range of not impairing the purpose according to the present invention, depending on the kind or purpose of use of the formulation in addition to the essential components in each formulation.

The cosmetic composition may generally include an acceptable carrier, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners, chelating agents, pigments, preservatives, fragrances, etc. may be appropriately blended, but is not limited thereto. no.

The acceptable carrier may vary depending on the formulation. For example, when formulated into ointments, pastes, creams or gels, carrier oils, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silica, talc, zinc oxide or theirs Mixtures can be used

When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or mixtures thereof may be used as the carrier component, and in the case of a spray, chlorofluorohydro It may further comprise propellants such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated as a solution or emulsion, a solvent, solubilizer, or emulsion agent may be used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-butylglycol oil may be used, and in particular, cottonseed oil, peanut oil, corn seed oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, fatty acid esters of polyethylene glycol or sorbitan may be used.

The cosmetic composition, when formulated as a suspension, includes a liquid diluent such as water, ethanol or propylene glycol as a carrier component, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters, and polyoxyethylene sorbitan esters, Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the cosmetic composition is formulated as a soap, as a carrier component, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars and the like Can be used.

The cosmetic composition may be a fatty material, an organic solvent, a dissolving agent, a thickening agent, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizing agent, a foaming agent, and a fragrance which are commonly used in the industry, depending on the quality or function of the final product. Commonly used in surfactants, water, ionic or nonionic emulsifiers, fillers, metal ion rod blockers, chelating agents, preservatives, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, cosmetics It may additionally contain adjuvants commonly used in the cosmetic or dermatological arts, such as any other ingredient.

However, the adjuvant and its mixing ratio may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

Through the following examples, the present invention will be described in more detail, but the present invention is not limited by the following examples.

Production Example  : Preparation of Natural Extracts

After washing with Jeju trees, gyoyo lamp, pear scent, dolomite, buckwheat buckwheat, goblin birch, tangerine and turmeric, completely dried at room temperature and pulverized to obtain a 100g pulverized product. For 100 g of each milled product, 20-fold volume of 70% ethanol was used as the solvent for 7 days at room temperature.

The extract was filtered through a 250 mesh and 3 ㎛ filter, concentrated under reduced pressure at 40 ℃ and lyophilized to obtain a solid of the extract.

Example  And Comparative example

In order to verify the skin improvement effect of the obtained sample, the samples were mixed as in Tables 1 and 2 to set Examples and Comparative Examples.

[Contents (parts by weight)] division Example One 2 3 4 5 6 7 8 9 Jeju Tree Extract 30 20 20 20 20 20 20 20 16 Gorilla lamp extract - 10 - - - - - - 2 Pear Herb Extract - - 10 - - - - - 2 Dolomite Extract - - - 10 - - - - 2 Buckwheat extract - - - - 10 - - - 2 Gobibigobi Extract - - - - - 10 - - 2 Wormwood Extract - - - - - - 10 - 2 Turmeric extract - - - - - - - 10 2

[Contents (parts by weight)] division Comparative example One 2 3 4 5 6 7 8 Gorilla lamp extract 30 - - - - - - 5 Pear Herb Extract - 30 - - - - - 5 Dolomite Extract - - 30 - - - - 4 Buckwheat extract - - - 30 - - - 4 Gobibigobi Extract - - - - 30 - - 4 Wormwood Extract - - - - - 30 - 4 Turmeric extract - - - - - - 30 4

Experimental Example  1: skin safety test

In order to verify the skin safety for the samples of the Examples and Comparative Examples, MTT assay experiments on fibroblasts (HDF), macrophages (RAW264.7), keratinocytes (HaCaT) was performed.

Samples of Examples and Comparative Examples were suspended in purified water for each concentration, and then cell viability was measured.

Cytotoxicity was performed by modifying Mosmann's method of measuring cell viability using MTT {3- (4,5-dimethylthiazol-2-yl) -2-5-diphenyltetrazolium bromide} reagent.

Fibroblasts (HDF), macrophages (RAW264.7), and keratinocytes (HaCaT) were each dispensed in 96-well plates at a concentration of 1 × 10 ⁴ cells / well for 48 hours at 37 ° C., 5% CO ₂ . Incubated.

After removing the culture medium and incubating the sample for 48 hours in a concentration-treated medium was removed the medium and washed repeatedly with PBS (Phosphate buffered saline).

MTT was dissolved in PBS at 5 mg / mL, 50 μL was added, and incubated for 48 hours at 37 ° C. and 5% CO ₂ . 100 L of DMSO (dimethyl sulfoxide) was added per well, stirred for 10 minutes, and the absorbance was measured at 540 nm.

As a result of the measurement, no cytotoxicity was confirmed at all concentrations of each sample. The results suggest that the sample is not only harmless to the human body but also has excellent human safety.

Experimental Example  2 : DPPH  Evaluation of free radical scavenging activity through measurement

The extracts of Examples and Comparative Examples were suspended in purified water to evaluate free radical scavenging acticity.

DPPH measurement is a method of measuring the absorbance at 540nm the degree of decolorization by scavenging the stable radical DPPH (2,2-Diphenyl-1-picrylhydrazyl radical).

Vitamin C was used as a control, and the concentration of 50% scavenging of radical DPPH (SC ₅₀ ) was measured and compared with the control (Table 3).

In particular, Example 1 measured the free radical scavenging activity for each concentration (Fig. 1).

[Concentration of 50% Scavenging of Radical DPPH] division SC ₅₀ ( μg / mL) Example 1 103.1 Example 2 96.6 Example 3 98.1 Example 4 91.5 Example 5 94.8 Example 6 98.6 Example 7 99.5 Example 8 96.1 Example 9 89.5 Comparative Example 1 158.4 Comparative Example 2 151.6 Comparative Example 3 156.8 Comparative Example 4 158.1 Comparative Example 5 152.6 Comparative Example 6 154.4 Comparative Example 7 158.3 Comparative Example 8 145.3 Control group (vitamin C) 8.7

Referring to Table 3, the lower the value of SC ₅₀ means that the scavenging activity of DPPH free radicals is higher. The higher the free radical scavenging activity is, the better the antioxidant effect can be evaluated. Samples 9 to 9 were significantly better in free radical scavenging activity than Comparative Examples 1 to 8.

In addition, Examples 2 to 9, including at least one extract of Jeju light tree, and gyyo lamp, baecho fragrance, baekwoon, buckwheat, Gobibibi, wormwood and turmeric extracts further improved the antioxidant activity compared to Example 1.

Experimental Example  3: ABTS  Evaluation of free radical scavenging activity through measurement

The extracts of Examples and Comparative Examples were suspended in purified water to evaluate free radical scavenging acticity.

ABTS is a measure of the conversion of antioxidants to colorless ABTS at 732 nm by eliminating the blue-green radical ABTS (2,2'-azinodi [3-ethlbenzthiazolne-6-sulfonic acid]).

Vitamin C was used as a control, and the concentration of 50% scavenging of the radical ABTS (SC ₅₀ ) was measured and compared with the control (Table 4).

In particular, Example 1 measured the free radical scavenging activity by concentration (Fig. 2).

[50% scavenging concentration of radical ABTS] division SC ₅₀ ( μg / mL) Example 1 74.4 Example 2 71.2 Example 3 72.1 Example 4 73.1 Example 5 72.5 Example 6 72.6 Example 7 72.4 Example 8 72.3 Example 9 75.6 Comparative Example 1 176.6 Comparative Example 2 181.2 Comparative Example 3 188.1 Comparative Example 4 184.4 Comparative Example 5 182.9 Comparative Example 6 184.3 Comparative Example 7 180.1 Comparative Example 8 170.1 Control group (vitamin C) 8.7

Referring to Table 4, the lower the value of SC ₅₀ means the higher scavenging activity of free radicals. The higher the free radical scavenging activity, the better the antioxidant effect. The sample of 9 had significantly better free radical scavenging activity than Comparative Examples 1-8.

In addition, Examples 2 to 9, including at least one extract of Jeju light tree, and gyyo lamp, baecho fragrance, baekwoon, buckwheat, Gobibibi, wormwood and turmeric extracts further improved the antioxidant activity compared to Example 1.

Experimental Example  4 : Elastase  Evaluation of anti-aging effect through inhibitory activity

The extracts of Examples and Comparative Examples were suspended in purified water at different concentrations to evaluate elastase inhibition activity.

Elastin, along with collagen, is a protein that maintains skin elasticity in skin connective tissue. Elastase is a protein-soluble enzyme that breaks down elastin, and inhibiting allasease inhibits the breakdown of elastin, thereby maintaining skin elasticity.

Add 140 μL of Tris buffer (pH 8.0), 20 μL of sample (500 μg / mL), and 20 μL of 5 mM N-Succinyl-Ala-Ala-p-nitroanilide (Sigma, S4760), then add 10 μL substrate solution. After mixing well with 20 μL of elastase (Sigma, E7885) of μL / mL and reacting at room temperature for 15 minutes, the amount of p-nitroaniline produced at 410 nm was measured.

In particular, Example 1 measured the elastase inhibitory activity for each concentration (Fig. 3).

division Elastase  Inhibitory activity ( % ) Example 1 38.9 Example 2 44.6 Example 3 43.3 Example 4 41.5 Example 5 45.1 Example 6 41.2 Example 7 44.4 Example 8 46.6 Example 9 48.7 Comparative Example 1 26.8 Comparative Example 2 25.6 Comparative Example 3 21.1 Comparative Example 4 26.6 Comparative Example 5 28.7 Comparative Example 6 21.5 Comparative Example 7 27.6 Comparative Example 8 23.5 Comparative Example 9 33.4 Kojic acid 45.2

The sample of the Example effectively inhibited the activity of the elastase as compared with the comparative example. Referring to Table 5, the samples of Examples 1 to 9 containing the extract of Jeju tree were significantly superior to the elastase inhibitory activity compared to Comparative Examples 1 to 9.

In addition, Examples 2 to 9, which include at least one extract of Jeju light tree and gyno lamp, baecho fragrance, baekwoon, buckwheat, Gobibibi, wormwood and turmeric extracts were further improved elastase inhibitory activity compared to Example 1.

Experimental Example  5: NO production inhibition evaluation

In order to confirm the anti-inflammatory effects of the Examples and Comparative Examples, nitric oxide (NO) formation inhibitory experiments were performed by the GRIESS method using RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, which are macrophages of mice, were passaged several times, placed in a 24-well plate for 3 × 10 ^{5 in} one well, and then cultured for 24 hours.

Example and Comparative Example Extracts were replaced with cell media containing diluted to a concentration of 200 μg / mL. At this time, the positive control group was incubated for 30 minutes by treating 2-amino-4-picoline, a NO production inhibitor, with 5 μM, and treated with 1 μg of LPS (Lipopolysaccharide) as a stimulator and incubated for 24 hours.

100 μL of the supernatant was taken and transferred to a 96-well plate, 100 μL of GRIESS solution was added thereto, and reacted at room temperature for 10 minutes. The absorbance at 540 nm was measured to determine the effect of inhibiting NO production of the extract. 6 is shown. In addition, the experiment was repeated three times.

In particular, Example 1 measured the anti-inflammatory activity for each concentration (Fig. 4).

division NO Generation rate ( % ) Example 1 46.2 Example 2 46.6 Example 3 41.3 Example 4 45.5 Example 5 44.1 Example 6 43.4 Example 7 41.2 Example 8 45.4 Example 9 33.6 Comparative Example 1 82.1 Comparative Example 2 84.5 Comparative Example 3 86.4 Comparative Example 4 81.6 Comparative Example 5 82.7 Comparative Example 6 83.5 Comparative Example 7 85.9 Comparative Example 8 79.6 Positive control 30.1 Negative Control (No Treatment) 100.0

Inhibition of NO production can be evaluated as having an excellent anti-inflammatory effect. The sample of the Example effectively suppressed NO generation as compared with the comparative example. Referring to Table 6, the samples of Examples 1 to 9 including the extract of Jeju tree was significantly superior to the NO production inhibitory effect compared to Comparative Examples 1 to 8.

In addition, Examples 2 to 9, which include at least one extract of Jeju light tree and gyno lamp, baecho fragrance, baekwoon, buckwheat, Gobibibi, wormwood and turmeric extract further improved the NO production inhibitory effect compared to Example 1.

Experimental Example  6: skin elasticity improvement evaluation

The skin elasticity improvement effect of the cosmetic composition containing the sample obtained by the Example and the comparative example was evaluated. Each sample was formulated with a cream of the same composition, and only the samples of Examples and Comparative Examples were different.

The creams of Examples and Comparative Examples, which were divided into groups and were divided into groups of healthy women 20 years or older (mean age 37 years) at a temperature of 24 to 26 ° C and a humidity of 75%, were applied.

The formulated cream was applied twice a day (morning and evening) around the eyes for 12 weeks, and skin elasticity was measured using a skin elasticity measuring instrument (Cutometer MPA580, Conrage + Khazaka, Germany). The test results are described as R8 (R8 (12 weeks) -R8 (0 weeks)) values of the cutometer MPA580, and R8 values indicate the properties of skin viscoelasticity, and the results are shown in Table 7 below.

division Skin elasticity improvement effect Example 1 0.65 Example 2 0.77 Example 3 0.72 Example 4 0.73 Example 5 0.72 Example 6 0.71 Example 7 0.73 Example 8 0.72 Example 9 0.78 Comparative Example 1 0.44 Comparative Example 2 0.42 Comparative Example 3 0.44 Comparative Example 4 0.44 Comparative Example 5 0.44 Comparative Example 6 0.45 Comparative Example 7 0.42 Comparative Example 8 0.52 Positive Control (Vitamin C) 0.92 Negative Control (No Treatment) 0.31

Referring to Table 7, the samples of Examples 1 to 9 containing the Jeju tree extract were remarkably superior to the skin elasticity improving effect compared to Comparative Examples 1 to 8. Further, the Jeju tree extract and gyno lamp, pear scent, baiyun grass, weak hair, Examples 2 to 9 comprising at least one of Gobi, wormwood and turmeric extract have improved skin elasticity improvement compared to Example 1.

Experimental Example  7: Evaluation of dermatitis improvement effect

The dermatitis improvement effect of the cosmetic composition containing the sample obtained by the Example and the comparative example was evaluated. Each sample was formulated with a cream of the same composition, and only the samples of Examples and Comparative Examples were different.

The creams of Examples and Comparative Formulations formulated for 340 selected women aged 20 to 60 years were evaluated and the extent of dermatitis improvement was evaluated.

For 340 women with similar dermatitis symptoms, the group was divided into 17 groups of 20 people and the formulated cream was applied to the entire face twice daily for 12 weeks at a temperature of 24 to 26 ° C. and 75% humidity.

At the end of the study, subjects were asked to complete a questionnaire and subjective efficacy evaluations were conducted. The questionnaire used to identify symptoms of dermatitis as "pruritus", "dry", "keratinosis", "dandruff", "erythema", "swelling", "skin crack", "tear and eczema", and "chondritis" After dividing and evaluating the degree of improvement for each symptom, the average of the symptom and the effect of improving the dermatitis symptom were evaluated for each individual.

division Significantly improved (persons) Minor improvements (persons) No improvement (persons) Example 1 3 2 5 Example 2 6 3 One Example 3 4 2 4 Example 4 4 2 4 Example 5 5 3 2 Example 6 4 2 4 Example 7 5 2 3 Example 8 4 3 3 Example 9 7 2 One Comparative Example 1 2 One 7 Comparative Example 2 2 2 6 Comparative Example 3 2 One 7 Comparative Example 4 3 One 6 Comparative Example 5 2 One 7 Comparative Example 6 One One 8 Comparative Example 7 One 2 7 Comparative Example 8 2 3 5

Samples of Examples 1 to 9 including Jeju tree extract were remarkably superior to the dermatitis effect compared to Comparative Examples 1 to 8. In addition, the extract of Jeju tree and the guinea lamp, pear scent, baiyun grass, buckwheat buckwheat, fern, wormwood and turmeric extract Examples 2 to 9 containing at least one has further improved the dermatitis improvement effect compared to Example 1.

The above description of the present invention is intended for illustration, and it will be understood by those skilled in the art that the present invention may be easily modified in other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the invention is indicated by the following claims, and it should be construed that all changes or modifications derived from the meaning and scope of the claims and their equivalents are included in the scope of the invention.

Claims (6)

Cosmetic composition comprising the extract of Jeju tree as an active ingredient. The method of claim 1,
Cosmetic composition further comprises one or more selected from the group consisting of gyoyo lantern extract, pear fennel extract, dolomite extract, buckwheat extract, goblin fern extract, cucurbita extract and turmeric extract. The method according to claim 1 or 2,
Cosmetic composition for antioxidant and anti-inflammatory. The method according to claim 1 or 2,
Cosmetic composition for wrinkle improvement. The method according to claim 1 or 2,
The extract is selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, acetone, ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3-butylene glycol Cosmetic composition extracted with one or more solvents. The method according to any one of claims 1 and 2,
Softening Cream, Nourishing Cream, Moisture Cream, Nourishing Cream, Massage Cream, Nourishing Lotion, Essence, Ampoule, Gel, Eye Cream, Oil, Foundation, Cleansing Cream, Cleansing Foam, Cleansing Water, Shampoo, Rinse, Pack, Spray and Powder Cosmetic composition formulated with one or more selected from the group consisting of.

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