KR20090056522A - Cosmetic composition having free radical scavenging activity comprising the extract of citrus aurantium, hypericum sampsonii hance, taxillus chinensis, ligustrum lucidum, lycium chinense as active ingredient - Google Patents

Abstract

A cosmetic composition containing a plant extract for preventing skin aging is provided to remove free radical, inhibit the oxidation of biomass, prevent skin aging. A cosmetic composition for removing free radical comprises 0.0001-20.0 weight% of Citrus aurantium, 0.0001-20.0 weight% of Hypericum sampsonii Hance, 0.0001-20.0 weight% of Taxillus chinensis, 0.0001-20.0 weight% of Ligustrum lucidum and 0.0001-20.0 weight% of Lycium chinense extract. The plant extracts are obtained by using water, hydrous and anhydrous low alcohol of 1-4 carbon atoms(methanol, ethanol, propanol and butanol), acetone, ethyl acetate, chloroform, or 1,3-butyleneglycol. The composition is used in a form of solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, powder type foundation, emulsion type foundation, wax type foundation or spray type foundation.

Description

 Cosmetic composition having free radical scavenging activity comprising the extract of citrus aurantium, hypericum sampsonii hance, taxillus chinensis, ligustrum lucidum, Lycium chinense as Active Ingredient}

The present invention relates to a cosmetic composition for free radical scavenging comprising one or more extracts selected from the group consisting of crypt, primrose, lychee, spermatozoon and phalanges, specifically, free radical scavenging action to prevent skin aging or The present invention relates to a cosmetic agent for free radical scavenging which is a delayed and natural substance.

In general, cosmetics keep the skin beautiful and healthy, and delay the aging of the skin as it ages. Aging of the skin is due to oxidative damage of biological material by free radicals. In other words, highly reactive free radicals such as hydrogen peroxide, hydroxy radicals, superoxide radicals, and other active oxygen radically oxidize the lipids that make up cell membranes, destroy cell membranes, oxidize proteins, and attack nucleic acids, causing oxidative damage. It destroys matter and eventually kills cells. Thus, preventing or delaying aging of the skin is made possible by eliminating free radicals in the skin. Ascorbic acid, DL-alpha-tocopherol, beta-carotene, and dibutyl hydroxyltoluene (BHT) are known to have free radical scavenging effects. have. Because these substances are mainly used as synthetics, they can cause skin safety problems for long-term use or in large amounts. Therefore, there is an increasing demand for free radical scavenging cosmetics that are safer for skin application.

The present inventors studied various plant extracts to search for substances that prevent skin aging, and found that extracts obtained from the stems or leaves of Gisae, primrose, lychee, spermatozoon, and phalanges were highly free radical scavenging. Thus, the present invention has been completed.

An object of the present invention is to provide a free radical scavenging cosmetics of natural substances that have a free radical scavenging action to prevent or delay skin aging, the cosmetics characterized by applying the free radical scavenging cosmetic composition to human skin To provide a method.

Other objects and advantages of the present invention will become apparent from the following examples and claims.

The present invention relates to a cosmetic composition for free radical scavenging containing at least one plant extract selected from the group consisting of crypts, primula, lychee, sperm, and phalanges. The present invention also relates to a cosmetic method characterized in that the cosmetic composition is applied to human skin. Since the plant extracts of the present invention have an excellent free radical scavenging effect and do not have the toxicity of the plant extracts themselves, the cosmetics containing the plant extracts can remove various free radicals in the skin generated by ultraviolet rays. It can protect the skin from branch skin damage.

The present invention provides a cosmetic composition for free radical scavenging containing one or more plant extracts selected from the group consisting of crypts, primula, herbaceous, sperm and phalanx.

In another aspect, the present invention provides a cosmetic composition for preventing skin aging containing at least one plant extract selected from the group consisting of crypts, primrose, the above life, jeongjaja and phalanges.

Citrus aurantium ( 운 ) is a plant of the fortune family, another name is tangerine. Leaves are alternate, egg-shaped oval, with many spots and wavy teeth, thick, with petiole broad wings. Fruits are round but slightly flattened, 6-8 cm in diameter, ripening yellowish brown in October-November. Flowers blossom in May, white, fragrant, one or several on axillas, 5 sepals and petals, 20-24 rodml stamens and 1 pistil. In Korea, it is grown as an orchard on Jeju Island and grows to about 7m. In oriental medicine, it is used to split immature fruit into two and used as an active ingredient in scpoparone, 5,6,7,8-Tetrahydroxy-3`, 4`-dimethoxyflavone, caffeine, limonin, etc. Known for

Primula ( Hypericum sampsonii Hance or Hypericum ascyron ) is a perennial herb with a dicotyledonous mucosa condylar musculaceae with a water lily. It is distributed in Korea, Siberia, China, and Japan, and lives in both grasses. The size grows up to 0.5 ~ 1.0m in height. Stems stand straight, square, branched, upper part green, lower part light brown, woody. Leaves are opposite each other, 5-10 cm in length, pointed, pointed, bottom wrapped around stem, with flat and transparent edges. Flowers bloom in June-August, 4-6cm in diameter, reddish on yellowish background, and run upward one by one on the end of the branch. In oriental medicine, the entire plant, except for the root, is used as a medicine called Hong Hannyeon (紅 旱 蓮), which is effective for headaches and hypertension due to liver dysfunction, hemostasis, and crushed on boils and spears and applied to the affected area. The pistil is 1cm long and splits about one-third from its tip, called var. Longistylum .

This is a medicinal herb (Korea) dried on leaves, stems, and branches of Mistletoe mulberry ( Taxillus chinensis, Loranthus parasiticus ) , a semi-parasitic plant that lives on other trees. . The ancestors were called parasitic, locust, and arboreal because they looked similar to the birds standing on them. The main components were beta-Amyrin and Ino. Inositol, Quercitrin, Viscotoxin and Oleanolic acid are known. Taken between winter and spring, cut off branches, dry or steam and dry.

Liguustrum lucidum refers to the fruit of the ash tree Ligustrum japonicum. Mine grows in the lower foothills of the beach. Branches of 3 ~ 5mfh height are gray and have clear trees. Leaves are 3 ~ 10cm, butterfly is 2.5 ~ 4.5cm, it is tough like leather, broad egg-shaped or egg-shaped long oval with pointed end and not sawtooth. In July-August, white flowers bloom in double inflorescences, 5-12 cm in length and butterfly at the end of the new branch. Fruits are long, rounded, with 8-10mm long, ripen with 10-11dnjfdp purple-black. In oriental medicine, dried fruit is used as a tonic medicine, and in folk medicine, the leaves are boiled and applied to boils. The main ingredients are rutinosides, oleanolic acid, quercetin and ursolic acid.

Phalanx is a dried herb of Lycium chinense Miller or other plant of the same genus (Galaceae and Solanaceae). In Japan, it refers to Lycium chinense Miller and Lycium barbarum L. In China, only the Ningxia journalists are used as herbal medicine.

Kishi, primrose, herbaceous, sperm and phalanx extract contained in the cosmetic of the present invention are extracted by the conventional extraction method from the branches, leaves, roots, fruits, rhizomes or outposts of these plants. According to a preferred embodiment of the present invention, Gisil is used to dry the young fruit of the tangerine, primula is used for all parts except the root, the life is using the branch, the woman uses the fruit, the phalanges of the root Extract using roots.

The plant extract used in the cosmetic composition of the present invention may be obtained through various methods known in the art, and preferably comprises (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (e.g., Methanol, ethanol, propanol, butanol and the like), (c) acetone, (d) ethyl acetate, (e) chloroform or (f) 1,3-butylene glycol. More preferably, the extraction solvent is ethanol, most preferably about 70% ethanol. The plant extract, which is a material for extraction, may be used by drying the branch or leaves of the plant or as it is, and preferably by drying. As a drying method, natural drying or a hot air drying method can be used.

On the other hand, it will be apparent to those skilled in the art that the extract of the present invention can be obtained not only the above-described extraction solvent, but also plant extracts having substantially the same effect using other extraction solvents.

In addition, the extract of the present invention includes not only the extract by the above-mentioned extraction solvent, but also the extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the plant extract of the present invention. The plant extracts of the present invention can be prepared in powder form by additional processes such as distillation under reduced pressure, freeze drying or spray drying.

The plant extract of the present invention has the activity of protecting the skin from skin damage caused by free radicals caused by external factors and the like.

The external factors include ultraviolet rays, stress, and external harmful chemicals.These factors generate free radicals (eg, hydroxyl radicals, superoxide radicals, free radicals, and hydrogen peroxide) to increase oxidative stress in the skin, causing inflammation, Skin cell death and skin cell DNA damage occur and as a result promote skin cancer development or aging of the skin.

The term "skin damage" of the present invention means that the skin does not perform its normal function and includes damage to skin components (such as collagen and elastin), impaired function of skin cells and death of skin cells.

The skin cells of the present invention include all cells present in the skin, preferably keratinocytes and fibroblasts.

The term "anti-aging of the skin" of the present invention includes the action of preventing skin damage and improving the damaged skin, thereby improving the skin wrinkles, improve skin elasticity, improve skin texture and the like.

The content of each plant extract included in the cosmetic composition of the present invention is 0.0001-20.0% by weight based on the total composition, preferably 0.01-10.0% by weight.

When the composition of the present invention is formulated as a cosmetic, the components included in the cosmetic composition of the present invention include components conventionally used in cosmetic compositions in addition to the plant extract as an active ingredient, such as antioxidants, stabilizers, solubilizers, vitamins Conventional adjuvants such as pigments and perfumes, and carriers.

The cosmetic composition for protecting the skin of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactants- It may be formulated as containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Soluble cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In another aspect, the present invention provides a cosmetic method comprising applying a cosmetic composition containing the plant extract to the human skin.

The cosmetic method of the present invention refers to all cosmetic methods for applying the cosmetic composition of the present invention to human skin. That is, all the methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in duplicate, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition with excellent skin protection effect according to the present invention can be used according to a conventional method of use, the number of times of use can be varied according to the user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be wiped off, peeled off or washed with water after application to the skin. As a specific example, the soap is liquid soap, powdered soap, solid soap and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towels and cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , Cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

When the cosmetic method comprising the plant extract of the present invention is applied to a human skin, a cosmetic method can be obtained, thereby providing an excellent anti-aging effect due to an excellent radical scavenging effect.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those of ordinary skill in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident.

Preparation Example 1 Preparation of Plant Extract

As a raw material of the present invention, as shown in Table 1 below, Ki-sil is prepared by washing and cutting 1,000 g of roots of young fruit of the tangerine, the outpost except the root of the primula, the branches of the above life, the fruit of the woman, and the root of the phalanx. Thereafter, 5,000 ml of 70% ethanol, in which the ratio of water and ethanol was adjusted to 7: 3, was added thereto and leached at room temperature for 3 days. It was filtered and the filtrate was concentrated in vacuo to give a dry extract. The dried extract was dissolved in 30% 1,3-butylene glycol solution to a concentration of 1% (w / v) and filtered and then applied to the experiment of the present invention.

Plant extracts Extraction site air lock Young fruit Circular guard Outpost (excluding root) Reminder Branch Journeyman Fruit Phalanges Root Root

Experimental Example 1: Free radical scavenging action

The free radical scavenging activity of the plant extracts prepared in Preparation Example 1 was measured as follows. That is, each plant extract was added to 100 ug / ml of each 60 ml of ethanol solution of 1,1-diphenyl-2-picryl-2-hydrazyl (DPPH). After reacting at room temperature for 30 minutes, absorbance was measured at 520 nm. Vitamin C was used as a comparative group. The free radical scavenging action was calculated by the following formula (1). The results are shown in Table 2 below.

Sample concentration (100ug / ml) Free radical scavenging effect (%) air lock 93.85 Circular guard 91.91 Reminder 90.63 Journeyman 93.20 Phalanges 92.38 Vitamin C (Control) 95.95

Through the results in Table 2, SC ₅₀ value of the concentration of the sample to eliminate 50% free radicals for each plant extract was obtained and the results are shown in Table 3 below.

Sample concentration (100ug / ml) Free radical cancellation effect (SC ₅₀ ) air lock 0.023% Circular guard 0.008% Reminder 0.0016% Journeyman 0.033% Phalanges 0.015% Vitamin C (Control) > 0.02%

As can be seen from the results of Table 3, the value of SC ₅₀ corresponding to the free radical scavenging activity of each of the plant extracts of Kishil, Woncho, the above life, the sperm, and the phalanges of the active ingredient of the present invention is similar to the vitamin C of the positive control. By confirming that the plant extracts of the present invention are high or free radical scavenging activity can be seen that significant.

 Experimental Example 2: Inhibitory effect on hydrogen peroxide-induced cytotoxicity

The inhibitory effect on the hydrogen peroxide-induced cytotoxicity of the plant extract prepared in Preparation Example 1 was measured using mouse-derived fibroblasts.

Hydrogen peroxide generates hydroxyl radicals in cells, causing lipid peroxidation, leading to cell membrane damage and cytotoxicity. The free radical scavenging activity of each plant extract was measured as follows to test at the skin cell level. Human skin fibroblasts of the logarithmic growth stage were inoculated in a 96-well plate at 10,000 cells per well, incubated in 80 µl of DMEM medium for 24 hours, and then diluted in the medium so that the final concentration in the medium was 10 ^-3 M. 10 μl of hydrogen peroxide was added, and at the same time, the final concentration of the plant extract of Preparation Example 1 was added to 100 ug / ml. After 24 hours, 10 μl of 5 mg / ml MTT solution (Sigma, United States of America) was added, and after 4 hours, dimethyl sulfoxide was added. The solution was added and dissolved in 200 μl per well, and then the absorbance was measured at 570 nm with a microplate reader.

In the same way, the survival rate of the cells treated only with plant extracts was also determined to determine whether the plants were cytotoxic.

Cell viability was calculated using Equation 2 below, and the results are shown in Table 4 below.

Sample concentration (100ug / ml) Cell survival rate (%) after hydrogen peroxide treatment Cell viability of the plant extract itself (%) Hydrogen peroxide treatment 0.0 - air lock 66.3 101.5 Circular guard 65.8 100.3 Reminder 67.3 99.8 Journeyman 68.8 99.9 Phalanges 69.7 99.3 Vitamin C (Control) 70.5 99.3

In the results of Table 4, it was confirmed that each of the plant extracts of Kishi, Woncho, said life, sperm, phalanges of the active ingredient of the present invention has an excellent effect of inhibiting cytotoxicity caused by hydrogen peroxide. In addition, the cell viability of the plant extract itself also showed a survival rate close to 100% including the error range to confirm that there is no toxicity of the plant extract itself. Through the above results, it can be seen that the plant extracts of the present invention effectively eliminate free radicals in the skin caused by ultraviolet rays, environmental pollution, and the like.

Formulation examples are shown below, but the present patent is not limited to the following formulation examples. In addition, the following plant extract was used in the extract of Preparation Example 1. Although only the plant extract is indicated in the following formulation example, it is a formulation example for each extract.

Formulation Example 1 Flexible Lotion (Skin)

Formulation examples of the flexible lotion (skin) in the cosmetic containing the plant extract obtained above is as follows.

ingredient Content (% by weight) Plant extracts 0.5 1,3-butylene glycol 5.2 Oleyl alcohol 1.5 ethanol 3.2 Polysorbate 20 3.2 Benzophenone-9 2.0 Carboxyl Vinyl Polymer 1.0 glycerin 3.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 2: Milk Lotion

Formulation examples of the milk lotion in the cosmetic containing the plant extract obtained above are as follows.

ingredient Content (% by weight) Plant extracts 0.6 glycerin 5.1 Propylene glycol 4.2 Tocopheryl Acetate 3.0 Liquid paraffin 4.6 Triethanolamine 1.0 Squalane 3.1 Macadamia Nut Oil 2.5 Polysorbate 60 1.6 Sorbitan sesquirrolate 1.6 Propylparaben 0.6 Carboxyl Vinyl Polymer 1.5 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 3: Nutrition Cream

Formulation example of the nutrient cream in the cosmetic composition containing the plant extract obtained above is as follows.

ingredient Content (% by weight) Plant extracts 1.0 glycerin 4.0 vaseline 3.5 Triethanolamine 2.1 Liquid paraffin 5.3 Squalane 3.0 Beeswax 2.6 Tocopheryl Acetate 5.4 Polysorbate 60 3.2 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 3.1 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 4: Massage Cream

An example of the formulation of the massage cream in the cosmetic containing the plant extract of the present invention is as follows:

ingredient Content (% by weight) Plant extracts 0.5 glycerin 4.0 vaseline 3.5 Triethanolamine 0.5 Liquid paraffin 24.0 Squalane 3.0 Beeswax 2.1 Tocopheryl Acetate 0.1 Polysorbate 60 2.4 Carboxyl Vinyl Polymer 1.0 Sorbitan sesquioleate 2.3 incense a very small amount antiseptic a very small amount Purified water Remaining amount system 100

Formulation Example 5: Pack

Formulation examples of the pack in the cosmetic containing the plant extract obtained above are as follows:

ingredient Content (% by weight)  Plant extracts 1.0 Ethyl alcohol 3.0 EDTA-2Na 0.02 Propylene glycol 5.1 Glyceline 4.5 Cabopol 1.0 Polyoxide 0.1 antiseptic a very small amount incense a very small amount Purified water Remaining amount system 100

Claims (6)

Free radical scavenging cosmetics containing one or more plant extracts selected from the group consisting of Citrus aurantium , Hypericum sampsonii Hance , Taxillus chinensis , Ligustrum lucidum and Lycium chinense Composition. A cosmetic composition for preventing skin aging containing at least one plant extract selected from the group consisting of crypts, primroses, the above-mentioned, sperm and phalanges. The cosmetic composition according to claim 1 or 2, wherein the plant extract prevents skin aging by eliminating free radicals in the skin generated by ultraviolet rays. 3. The cosmetic composition according to claim 1 or 2, wherein the content of the plant extract is 0.0001-20.0 wt% based on the total weight of the cosmetic composition. The composition of claim 1 or 2, wherein the composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation and Cosmetic composition, characterized in that the formulation is selected from the group consisting of a spray. The cosmetic composition according to any one of claims 1 to 5, wherein the cosmetic composition is applied to human skin to prevent skin aging.