KR102168533B1 - Cosmetic composition for whitening or improving the facial color containing herb extracts - Google Patents

Abstract

The present invention relates to a composition for whitening or improving complexion. The composition comprising the extract of Angelicae, Cornus, antler, Sun thyme or a mixture thereof according to the present invention as an active ingredient can be used as a cosmetic composition for whitening or improving complexion by exhibiting a whitening effect by inhibiting melanin synthesis.

Description

Cosmetic composition for whitening or improving the facial color containing herb extracts}

The present invention relates to a cosmetic composition for whitening or improving complexion comprising an herbal extract, and more particularly, comprising one or more extracts selected from the group consisting of Angelicae, Cornus, antler and Sun thyme as an active ingredient. It relates to a cosmetic composition for whitening or improving complexion.

Melanin, which determines the color of human skin, is produced in melanocytes. Specifically, melanin is a dark brown pigment formed by polymerization and oxidation reactions by enzymes such as tyrosinase present in melanocytes using tyrosine, an amino acid present in vivo, as a substrate. The melanin formed in this way moves to epidermal cells called keratinocytes through dendritic processes of melanocytes. Melanin that has migrated to keratinocytes can be removed by releasing them together from the skin as the keratinocytes break away from the epidermis. However, since there is no enzyme that degrades melanin in the living body, melanin once formed is not degraded in vivo. Therefore, in order to brighten the skin, it can be said that suppressing the production of melanin is the key.

The need for research on whitening agents as cosmetics is gradually increasing as the living standards of the Eastern world, which emotionally prefer white skin, are improved, and skin blackening is recognized as skin aging due to ultraviolet rays. Accordingly, substances exhibiting tyrosinase inhibitory activity, such as ascorbic acid, hydroquinone, glutathione, and arbutin, have been formulated and used in cosmetics and pharmaceuticals (Patent Document 1 and 2) Most of these have insufficient effects or are unstable in their formulation, so their utilization is poor. In particular, compounds such as hydroquinone exhibit a strong bleaching action and have skin sensitization by themselves, which can cause skin allergies and other side effects, such as causing vitiligo by changing the normal skin function. Its use is limited in.

In addition, even if it is a substance that inhibits the activity of tyrosinase as described above, if these substances act on keratinocytes, which are keratinocytes, to increase the production of factors that promote the biosynthesis of melanin, as a result, the whitening effect is weakened or rather Since it can have an adverse effect, simply an inhibitor of tyrosinase activity cannot be considered as a good whitening agent. Accordingly, many tyrosinase inhibitors have been developed, but most of them are known to exhibit no excellent whitening effect.

Under this background, the present inventors have conducted research on substances that are not toxic from natural resources and have excellent skin condition improvement effect, and thus at least one selected from the group consisting of angelica, cornus, antler and thyme It was found that the herbal medicine extract exhibits the inhibitory effect of melanin synthesis, and a cosmetic composition for whitening or improving complexion using it has been completed.

Korean Registered Patent Publication No. 10-0923141 Korean Registered Patent Publication No. 10-0921947

It is an object of the present invention to provide a cosmetic composition for whitening or improving complexion, characterized in that it comprises one or more extracts selected from the group consisting of angelfish, cornus, deer antler and sun thyme as an active ingredient.

In order to solve the above problems, as an embodiment of the present invention, whitening or complexion improvement, characterized in that it comprises one or more extracts selected from the group consisting of angelfish, cornus, antler and sun thyme as an active ingredient. It provides a cosmetic composition for.

In another embodiment of the present invention, it provides a cosmetic composition for whitening or improving complexion, characterized in that it comprises a mixed extract or a fraction thereof of an angelfish, cornus milk, antler and sun thyme as an active ingredient.

In another embodiment of the present invention, the mixing ratio of the angelfish, cornus milk, antler and thyme is 10 to 40: 10 to 40: 10 to 40: 10 to 40, more preferably 20 to 30: 20 by weight. To 30: 20 to 30: it may be 20 to 30.

Angelica acutiloba of the present invention is a dried root of Daehwa Angelica, a perennial herb belonging to the Parsley family, and is also called Japanese Angelica, and its taste is sweet and very, and its nature is warm. Ildangwi was first introduced and cultivated for export to Japan, but in recent years, it has been in the spotlight as a functional ssam vegetable with its unique fragrance and rich minerals and vitamins on its leaves. In terms of efficacy, useful pharmacological effects such as neuroprotective effects, analgesics, and anti-inflammatory effects are known.

Corni Fructus of the present invention belongs to the dogwood family, and when the fruit matures in October to November, it is collected, removed branches and stems, dried over low heat, cooled, and discarded, the seeds are discarded, and the pulp is dried again in sunlight or low heat, and shade Keep in. In oriental medicine, it is used as a nutrient, tonic, and astringent medicine, and the main ingredients are Moroniside, Loganin, Cornin (Verbenalin), Saponin, Tannin, and ursolic acid. (Ursolic acid), gallic acid (Gallic acid), mariic acid (Malic acid), tartaric acid (Tartaric acid) and vitamin A, etc. are contained.

The antler (cervi parvum cornu) of the present invention is a herbal medicine that has been widely used as a blood tonic for a long time as a dried herbal medicine obtained by collecting and processing freshly grown horns of a stag. As a result of animal and clinical trials, deer antler was found to have an effect of promoting growth and development in children, an anti-aging effect, an effect of hematopoietic function, an effect of improving heart function, an anti-muscular fatigue effect, and an anti-pain effect.

The thyme of the present invention (Thymus vulgaris) is a perennial evergreen tree that grows to about 45cm and has many branches. The leaves are grayish green and have an aromatic scent, and the flowers are pale purple or white. It is mainly used for respiratory and digestive problems, and the British Herbal Pharmacopoeia says that it can be used for indigestion, chronic gastritis, and bronchitis.

As used in the present invention, the term "extract" refers to an extract obtained by extraction treatment of the Angelica Angelica, Cornus deer antler, Sun thyme or a mixture thereof, a dilution or concentrate of the extract, a dried product obtained by drying the extract, and It includes the extract of all formulations that can be formed using the extract itself and the extract, such as a crude product, a purified product, or a mixture thereof.

The extract of the present invention may be extracted from natural, hybrid or variant plants of the respective plants, and may be extracted from plant tissue culture.

In the extraction of the angelfish, cornus, antler, thyme or a mixture thereof of the present invention, the extraction method is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, which may be performed alone or in combination of two or more methods.

In the present invention, the kind of the extraction solvent used to extract the mono angelica, cornus oil, antler, thyme or a mixture thereof is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; C ₁ to C ₄ lower alcohols such as methanol, ethanol, propyl alcohol, and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof may be used, and preferably, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in combination of two or more. In the present invention, more preferably, water (or distilled water), 1,3-butylene glycol, or a mixed solvent thereof may be used as a solvent for extracting herbal medicinal herbs such as Angelicae, Cornus oil, Deer antler, and Sun thyme. Using the solvent, a solvent extract can be prepared by extracting medicinal herbs such as Angelicae, Cornus, antler, and Seon thyme at least once, and a dry extract obtained by distilling the solvent extract under reduced pressure and then freeze drying or spray drying can be prepared. have. In a specific embodiment of the present invention, a monosaccharide extract using an extraction solvent (distilled water: 1,3-butylene glycol = 7:3); Cornus extract; Antler extract; Sun thyme extract and mixed extracts of Japanese angelica, cornus, antler and sun thyme were prepared (Examples 1 to 5).

In the present invention, the extract extracted with hot water or cold needle is filtered to remove floating solid particles, for example, nylon, etc. to filter the particles, or after filtration using a freeze filtration method, etc., or use as such, or freeze drying, hot air drying, It can be used after drying by spray drying or the like.

As used herein, the term "fraction" means a result obtained by performing fractionation in order to separate a specific component or a specific component group from a mixture containing several different constituents.

The fractionation method for obtaining the fraction in the present invention is not particularly limited, and may be performed according to a method commonly used in the art. Non-limiting examples of the fractionation method may include a method of obtaining a fraction from the extract by treating an extract obtained by extracting Angelicae, Cornus, antler, Sun thyme, or a mixture thereof with a predetermined solvent.

In the present invention, the type of solvent used to obtain the fraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the fractionation solvent include polar solvents such as water and alcohol; Non-polar solvents, such as hexane (Hexane), ethyl acetate (Ethyl acetate), chloroform (Chloroform), and dichloromethane (Dichloromethane), etc. are mentioned. These may be used alone or in combination of two or more. In the case of using an alcohol in the fractionation solvent, preferably a C ₁ to C ₄ alcohol may be used.

Angelicae extract, cornus oil extract, deer antler extract, thyme extract or a mixed extract of antler, cornus deer antler and sun thyme of the present invention has the effect of effectively inhibiting the production of melanin, and specifically the effect of reducing the total amount of melanin synthesized Represents. In addition, since the composition of the present invention is a material derived from a natural product, it is not toxic, and even when applied to the human body, side effects are not caused, so it can be used as a cosmetic composition.

Melanin is a dark brown pigment produced from melanocytes, which determines the color of human skin, and can be expected to brighten skin by inhibiting melanin pigment.

In relation to the above efficacy, according to a specific embodiment of the present invention, in mouse melanoma cells (B-16 mouse melanoma cells), a mixture of an angelfish extract, cornus milk extract, antler extract, sun thyme extract or an angelfish, cornus milk, antler and sun thyme When the extract was treated by concentration, it was confirmed that the total amount of melanin in melanoma cells was reduced (Example 6).

In particular, the extract of the mixture containing all of the Angelicae, Cornus, deer antler and Sun thyme of the present invention or a fraction thereof is a single extract of each plant by synergistic or cooperative action between the extracts and/or the active ingredients in the extract Compared to that, it can exhibit a more excellent skin whitening and complexion improvement effect, which was first identified in the present invention.

Specifically, the extract of the mixture containing all of the Angelicae, Cornus, deer antler and Seon thyme of the present invention, or a fraction thereof, further reduces the total amount of melanin in melanoma cells compared to the sole extract of Angelicae, Cornus, Deer antler or Seon Thyme. It may exhibit more excellent skin whitening and complexion improvement effects.

In the present invention, the mixing ratio of the angelica, cornus, antler and thyme is preferably 10 to 40: 10 to 40: 10 to 40: 10 to 40, more preferably 20 to 30: 20 to 30 by weight. : 20 to 30: It may be 20 to 30.

According to a specific embodiment of the present invention, as a result of investigating the degree of reduction in the total amount of melanin in the melanoma cells by preparing a mixed extract having a blending ratio of the angel angelfish, cornus milk, antler and sun thyme being 25:25:25:25, melanoma It was confirmed that the degree of reduction in the total amount of melanin in norma cells was greater than that of the single extracts of Angelica, Cornus, antler, or Seon thyme (Example 6).

The term "whitening" used in the present invention refers to any action of inhibiting or preventing melanin deposition by inhibiting the synthesis of melanin.

As used herein, the term "improving complexion" means that skin blemishes such as spots, freckles, and age spots on the face are removed or produced is suppressed, and the skin tone is brightened, so that the overall color of the face is improved aesthetically.

The cosmetic composition of the present invention is a solution, external ointment, cream, foam, nutrient lotion, flexible lotion, pack, softening water, emulsion, makeup base, essence, soap, liquid detergent, bathing agent, sunscreen cream, sun oil, suspension, emulsion It can be prepared in a formulation selected from the group consisting of liquid, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch, and spray, but is limited thereto. no.

The cosmetic composition of the present invention may additionally include one or more cosmetically acceptable carriers blended in general skin cosmetics, and as common ingredients, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners , A chelating agent, a colorant, a preservative, a fragrance, and the like may be appropriately blended, but are not limited thereto.

The cosmetically acceptable carrier included in the cosmetic composition of the present invention varies depending on the formulation of the cosmetic composition.

When the formulation of the present invention is an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohard It may include a propellant such as locarbon, propane/butane or dimethyl ether, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, and the like may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan May be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, micro Crystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used, but are not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a soap, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, an aliphatic alcohol, vegetable oil, glycerol, sugar, etc. are used as a carrier component. It may be, but is not limited thereto. These may be used alone or in combination of two or more.

In the cosmetic composition of the present invention, the herbal extracts of Angelicae, Cornus, Deer antler, Sun thyme or a mixture thereof may be included in a dry weight, 0.0001 to 50% by weight of the total weight of the cosmetic composition, and more preferably 0.0005 It is preferably included in to 10% by weight. There is an advantage of showing excellent whitening and complexion improvement efficacy within the above range, and there is an advantage of stabilizing the formulation of the composition.

The composition containing the extract or a fraction thereof of the Angelicae, Cornus, antler, Sun thyme or a mixture thereof of the present invention is excellent in skin whitening effect. Specifically, the composition of the present invention is excellent in the efficacy of inhibiting the increase in the total amount of melanin.

In addition, since the composition of the present invention is a material derived from a natural product, it is not toxic, and even when applied to the human body, it does not cause side effects, so its utilization as a cosmetic composition is high.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are for illustrative purposes only, and the scope of the present invention is not limited by these examples.

Example One: Of the daily angelica extract Produce

After drying and slicing, 100 g of dry weight was added to a flask and extracted by cold soaking for 3 days with 1000 g of an extraction solvent (distilled water: 1,3-butylene glycol = 7:3). The cold-precipitated extract was filtered through a filter having a pore size of 0.2 µm to prepare a daily angelica extract.

Example 2: Preparation of Cornus oil extract

After drying and mincing the cornus oil, 100 g of dry weight was put into a flask and extracted by cold soaking for 3 days with 1000 g of an extraction solvent (distilled water: 1,3-butylene glycol = 7:3). The cold-precipitated extract was filtered through a filter having a pore size of 0.2 µm to prepare a cornus oil extract.

Example 3: Preparation of antler extract

After the antler was dried and minced, 100 g of dry weight was put into a flask, and extracted by cold soaking with 1000 g of an extraction solvent (distilled water: 1,3-butylene glycol = 7:3) for 3 days. The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare an antler extract.

Example 4: Sun thyme extract Produce

After drying and mincing the sun thyme, 100 g of dry weight was put into a flask, and extracted by cold soaking for 3 days with 1000 g of an extraction solvent (distilled water: 1,3-butylene glycol = 7:3). The chilled extract was filtered through a filter having a pore size of 0.2 μm to prepare a sun thyme extract.

Example 5: An angel , Cornus, antler and Thyme Preparation of mixed extract

After drying and slicing the angelica, cornus oil, antler and thyme, 100g of dry weight (25g of cornflower, 25g of cornus, 25g of deer antler, 25g of sun thyme) was added to the flask and extraction solvent (distilled water: 1,3-butylene glycol = 7 :3) 1000g was extracted by cold soaking for 3 days. The cold-precipitated extract was filtered through a filter having a pore size of 0.2 µm to prepare a mixed extract of Japanese angelfish, cornus milk, antler and sun thyme.

Example 6: Whitening effect-Investigation of the effect of reducing the total amount of melanin

The extracts of Examples 1 to 5 were added to the culture medium of mouse melanoma cells (B-16 mouse melanoma cells) to test the whitening effect at the cellular level. For comparison, no separate treatment was applied to the negative control group, and arbutin was added to the positive control group.

Specifically, the final concentration (% by weight) of the herbal medicine extracts of Examples 1 to 5 was tested to be 0.1% or 1%, and arbutin, a positive control, was added to the medium so that it became 200 μg/ml, respectively, and B-16 The melanoma cells were treated and cultured for 3 days.

Thereafter, the cells were treated with trypsin, removed from the culture vessel, centrifuged, and melanin was extracted. The detached cells were boiled for 10 minutes by adding 1 ml of sodium hydroxide solution (1N concentration) to dissolve melanin, and the absorbance was measured at 400 nanometers (nm) using a spectrophotometer to measure the amount of melanin produced.

The amount of melanin was measured by a method of expressing the absorbance per unit cell count (10 ⁶ cells), and was calculated as an inhibition rate (%) for the total amount of melanin relative to the negative control, and the results are shown in Table 1 below. Also, the experiment was repeated 3 times.

sample(%) Total amount of melanin ( Abs ) Inhibition rate (%) Control (no addition) 0.082 - Arbutin (200ug/ml) 0.042 48.78 Example 1 (0.1%) 0.071 13.4 Example 1 (1.0%) 0.069 15.9 Example 2 (0.1%) 0.068 17.1 Example 2 (1.0%) 0.067 18.3 Example 3 (0.1%) 0.075 8.5 Example 3 (1.0%) 0.070 14.6 Example 4 (0.1%) 0.069 15.9 Example 4 (1.0%) 0.058 29.3 Example 5 (0.1%) 0.051 37.8 Example 5 (1.0%) 0.045 45.1

As can be seen from the results of Table 1, the daily angelica root extract, cornus oil extract, antler extract, sun thyme extract, and mixed extracts of these herbal medicines have the effect of reducing the total amount of melanin by inhibiting melanin synthesis, and by mixing four herbal medicines In the case of the extracted Example 5, it was found that there is an effect of further significantly reducing the total amount of melanin compared to each of the herbal extracts alone, and it was confirmed that the total amount of melanin reduction effect was similar to that of arbutin, a conventional whitening substance.

Manufacturing example 1: Preparation of essence containing the mixed extract of the present invention

A cosmetic composition of an essence formulation was prepared as shown in Table 2 below (unit: wt%).

Raw material name Content (% by weight) Cetearyl alcohol
Cetearyl glucoside
Jojoba seed oil
Silica
glycerin
Example 5 extract
Purified water 2
One
5
0.5
10
5
To 100

Manufacturing example 2: Preparation of a cream containing the mixed extract of the present invention

As shown in Table 3 below, a cream-formed cosmetic composition was prepared (unit: wt%).

Composition Content (% by weight) Example 5 extract
Stearic acid
ethanol
Potassium hydroxide
glycerin
Propylene glycol
antiseptic
incense
Purified water 5
15.0
1.0
0.7
5.0
3.0
Appropriate amount
Appropriate amount
to 100

Claims (7)

A cosmetic composition for whitening or improving complexion, characterized in that it comprises an extract of sun thyme or a fraction thereof as an active ingredient.
The cosmetic composition for whitening or improving complexion according to claim 1, comprising a mixed extract of Japanese angelica, cornus milk, antler and sun thyme, or a fraction thereof as an active ingredient.
The cosmetic composition for whitening or improving complexion according to claim 2, wherein the mixing ratio of the angelica branch, cornus milk, antler and sun thyme is 10 to 40:10 to 40:10 to 40:10 to 40 based on weight.
The cosmetic composition for whitening or improving complexion according to claim 3, wherein the mixing ratio of the angelfish, cornus milk, antler and sun thyme is 25:25:25:25 based on weight.
The method of claim 1, wherein the extract is obtained by extracting sun thyme with at least one solvent selected from the group consisting of water, C ₁ to C ₄ lower alcohol, 1,3-butylene glycol, and ethyl acetate. Cosmetic composition for whitening or improving complexion, characterized in that.
The cosmetic composition for whitening or improving complexion according to claim 5, wherein the solvent is a mixture of water and 1,3-butylene glycol in a weight ratio of 7:3.
The cosmetic composition of claim 1, wherein the whitening or complexion improvement is due to a decrease in the total amount of melanin in melanoma cells.