KR20170137405A - Composition for improving skin condition comprising herb extracts mixture - Google Patents

Abstract

The present invention relates to a composition which contains composite medicinal herb extracts as active ingredients, excellently improves skin condition, and is safe to be applied to skin. More specifically, the composition, according to the present invention, contains a Rehmanniae Radix Recens extract, a mume fruit extract, a Schisandra chinensis (Turcz.) Baill. extract, a liriope rhizome extract, a zedoaria extract, a Gleditsia sinensis Lamarck extract, and a Spatholobi Caulis extract as the active ingredients, thereby bringing outstanding skin condition improvement effects such as anti-saccharification effects, skin-whitening effects, skin elasticity and wrinkle amelioration effects, antioxidant effects, and alleviation effects for troubled skin. In addition, a composition containing a Ranunculus ternatus Thunb. extract in addition to the composite extract shows excellent effects regarding proliferation of fibroblast.

Description

[0001] The present invention relates to a composition for improving skin comprising a herbal extract,

The present invention relates to a composition containing as an active ingredient a complex herbal extract which is safe when applied to skin and has excellent skin improving effect.

The skin is an important body that is responsible for various physiological functions such as barrier function, body temperature control function, excretion function, which protects the human body from the external environment and prevents the internal moisture and useful components from flowing out. However, due to the following reasons, the activity of skin cells may deteriorate and the skin condition may deteriorate.

Collagen and elastin present in the dermis of the skin perform functions such as mechanical rigidity of the skin, resistance of the connective tissue, maintenance of the cohesion of the tissue, and support of the cell adhesion, and have a great influence on the constituents of the skin. It is known that such collagen is reduced by photoaging due to stress, aging, or ultraviolet irradiation, while elastin is known to be distorted in its three-dimensional structure by the degrading enzyme elastase, which is activated after exposure to ultraviolet light. As a result, the skin tissue is loosened and the elasticity is lost.

When the cell function of the epidermis is lowered, the metabolism is not smooth and the exfoliation does not occur well, and when the ultraviolet rays are received in a state where the keratin is overly accumulated, the elasticity is lowered and wrinkles are generated. If the skin is not formed on the epidermis, moisture and sebum secretion are reduced, and the skin becomes dry and wrinkles are formed.

When the skin receives ultraviolet rays, melanin is synthesized to protect the skin. The synthesized melanin is transferred to the keratinocytes of the skin through the melanoma. This keratinocyte turns over over a period of 28 days. Therefore, the melanin produced is generally lost by the keratinocyte periodically for 28 days. However, when the skin cell cycle is not properly controlled by the stress, skin aging, etc., keratinocytes do not fall out in the presentation period and the pigment such as spots, freckles, Calm occurs.

In the skin, sebum, sweat and cosmetic ingredients are decomposed into substances that are toxic to the skin by the fungus, which can cause skin irritation and inflammation. In addition, skin irritation caused by ultraviolet rays increases the production of nitrogen monoxide (NO), which is an inflammation mediator, to cause skin troubles. The production of nitrogen monoxide is mostly caused by iNOS, and it is known that iNOS is rapidly induced by stimulation such as LPS and cytokine to produce excessive NO.

A reactive oxygen species (ROS), also known as a toxic oxygen species, is a cell-generated toxic substance produced by physiological actions such as respiration and is constantly produced and extinguished, with 3-5% in normal conditions . These reactive oxygen species are free radicals such as superoxide radicals (O2-) and hydroxyl radicals (HO +), which are chemically bound to atoms or molecules that are not paired with the outermost electron orbits Highly unstable and highly reactive) or in the form of compounds with paired electrons such as hydrogen peroxide (H ₂ O ₂ ) or singlet radicals. Active oxygen species have the advantage of biologically protecting bacteria in the physiological system, but generally cause oxidation in vivo, causing harmful effects that cause disease. It has been reported that these reactive oxygen species attack biological molecules and damage cells and tissues and cause various diseases related to aging and various diseases.

Research has been continued to suppress the skin aging caused by various factors as described above, to improve whitening, wrinkles, elasticity or trouble, and to develop a substance having an antioxidative effect.

Materials having the above-mentioned effects are widely distributed in the natural world, and they have been mainly used as raw materials for foods, cosmetics, medicines and the like using materials derived from plants. However, the substances derived from such natural substances are not effective enough to be used in large quantities in order to obtain a meaningful effect, resulting in toxicity and price increase.

In order to solve the problems of such natural materials, the development of chemically synthesized materials has continued. Although they have a merit that they exert a superior effect even when used in a small amount compared to a natural substance, their use is limited due to a fatal problem that can cause large and small side effects to the human body.

Accordingly, it is inevitable to develop a substance derived from a natural product and having stability, while exhibiting an excellent effect on skin improvement.

A problem to be solved by the present invention is to provide a complex herbal medicine extract which is derived from natural materials and has stability, while exhibiting excellent effects on skin, in particular whitening, wrinkle, elasticity, trouble improvement and antioxidation.

In order to solve the above-mentioned problems, the present invention provides a method for skin whitening, wrinkle, elasticity, trouble, or antioxidation, which comprises extracts of raw persimmon extract, oatsuchu extract, omija extract, mukmun dong extract, To provide a composition (e. G., A cosmetic composition).

The present invention also provides skin whitening, wrinkle, elasticity, trouble, antioxidant, or fibroblast proliferation, which comprises as an active ingredient an extract of raw persimmon extract, oatsuchu extract, omiza extract, mukmun dong extract, The present invention provides a cosmetic composition.

[extract]

It is a fresh name of Rehmanniae Radix Recens and is a fresh root of Rehmannia glutinosa Liboschitz ex Steudel (ginseng and Scrophulariaceae). Its flavor is slightly worn and its quality is very cold. It is known that it acts to promote coagulation (coagulation), strong heart action, diuretic action, hypoglycemic action, and hypertension.

Ohmu (梅 梅) is a plum tree Prunus mume Siebold et Zuccarini (rosaceae Rosaceae) as the less ripe fruit will be seized. Its appearance is uneven, spherical or lumpy, and its outer surface is black or blackish brown with shriveling and no gloss. In the flesh, there is seed in the hard, flat oval nucleus. The drug should be large, thick and thick, and black. There is a peculiar smell and the taste is good. Immune enhancement, antimicrobial activity, and cervical cancer suppression have been reported as pharmacological actions.

Schisandra (五味子, Schisandra Fruit) trees are Schisandra Schisandra Chinensis is the fruit of Baillon (Schizandraceae). It is a ball shaped with a diameter of about 1cm and a dark red color. It contains 1-2 drops of red juice and reddish brown seeds. It has five flavors: sweet, sour, bitter, salty, and spicy. There are Omija (Northern Omija), South Omija, and Black Omija. Omiza grows mainly in the area of Taebaek Mountain, Namimiza grows in the southern island region, and black omija grows in Jejudo. It is produced in Korea, Japan, Sakhalin Island and China.

Liriope Tuber is a Liriope platyphylla Wang et Tang, or Ophiopogon japonicas Ker-Gawler (lily and Liliaceae), the bulge of the roots. The roots of the root of the gumboongdong are square or round square, 12 ~ 40mm in length and 4 ~ 9mm in diameter. The outer surface is yellowish white and has vertical wrinkles. The vagina is soft and chewy. The folded surface is yellowish white, slightly transparent, and the center is thin, strong and strong. The root bulge of the lobulus is a long quadrangular prism or fusiform, 10 to 25 mm in length and 3 to 5 mm in diameter. One end is pointed and the other a little round. Outer surface is light yellow to light tan and has large and small vertical wrinkles. It is easy to break, and the folded side is yellowish white, slightly transparent, and the center is narrow, sturdy and sturdy. Flowers bloom in May-August, purplish and 3 ~ 5 on the nodes of inflorescence. Flower ears are 8 ~ 12cm long and have nodes on small flower branches. It is top of the ovary and the fruit is round and early in the capsule, and the peel is peeled off early, so the seed is exposed and it is purplish black. The roots are used as medicines in oriental medicine, and they are used as anti-inflammatory, tonic, shinhae, expectorant, and cardiovascular agents. It is widely distributed not only in Korea, Japan, China, Taiwan and Northeast Asia but also worldwide.

Zedoary is a curcuma (蓬 莪 朮) Curcuma phaeocaulis Val., Kwangseon-eup (廣西 莪 朮) Curcuma kwangsiensis SG Lee et cf Liang or 금 金Curcuma wenyujin YH Chen et C. Ling (ginger and Zingiberaceae) roots are steamed or dried by steam. It has the effect of eliminating hemorrhage, eliminating blood, and helping the circulation of ki. This is a rootstock, ovate, long circular, conical or long spindle, 2 ~ 8 cm long, 15 ~ 40 mm in diameter. Outer surface is yellowish gray to grayish brown, the upper part is mostly pointed, but the lower part is dull circular. There is a slight peculiar smell and the taste is a bit spicy. Kwangseongjak (廣西 莪 朮) is a root stem with a slight protrusion of the node, and the cut surface is yellowish brown to brown, usually light yellow powder is attached. The ring pattern of the endothelial layer is yellowish white. Onurugum is a yellowish-brown to dark brown surface cut with rootstock and usually has light yellow to yellowish brown powder. There is a slight peculiar smell.

The sculptor (Gleditsia Spine) is the main tree Gleditsia japonica Miquel var. koraiensis Nakai or carved wood Gleditsia sinensis Lamark is visible in (beans and Leguminosae). Its appearance is visible, divided into several pieces, and divided into small pieces. The outer surface is reddish purple or reddish brown, and the tip of the spine is reddish brown with glossy and thin wrinkles. The vagina is hard and not easily broken, and it is good that it is large and hard. Pharmacological action of acute tonsillitis, fever and inflammation has been reported. There is no smell, the taste is spicy and the quality is warm.

Spatholobi Caulis is a cinnabar stalk of Spatholobus suberectus Dunn (leguminosae). It is a cuneiform stalk, flat cylindrical, slightly bent, 30 ~ 40cm long, 3 ~ 5cm in diameter. Outer surface is grayish brown, has vertical grooves and horizontal skin, has slightly protruding nodes, and has marks on branches. The cork layer is reddish brown. On the folded side, there is a slanting number (髓), the neck is light reddish brown, and the serpent is a ring of reddish brown to blackish brown. On the outside of the temple there are three or more concentric rings arranged alternately in the neck and the temple. There is a peculiar smell and there is a taste.

The toadstool (猫 爪 草) is a frog fresh Ranunculus It is ternatus Thunb. In Korea, it grows in wetlands near Mt. Seorak and Mt. Halla. It is 10 ~ 25cm in height. There are rounded roots and beard roots with sharp ends at both ends. The leaves on the roots are long petiole, palmate, and three deep or fully cracked. The small leaf is egg shaped in inverted shape, there are sawtooths, and there are 3 pieces again. There are 1 to 4 leaves on the stem, no petiole, and completely divide into 3 pieces. Yellow flowers bloom in April-May, and small peduncles are 1.5-3cm long. Calyx leaves are 5, wide elliptical, slightly concave and hairy on the back. There are 5 petals in the shape of inverted egg and there is honey gland at the bottom. The operating table is about 1.8mm long and has no hairs. Fruits are spherical and round shaped with a diameter of about 1.2mm. Used for the treatment of pulmonary tuberculosis and tuberculous lymphadenitis. It is distributed in Korea, Japan, and Taiwan.

The present inventors have found that all of the above extracts have a biological activity beneficial to the skin and extracts of two or more of the above extracts, preferably extracts of Oriental persimmon, Omega extract, Omija extract, Mamunmundo extract, Extract extract, Sculpture extract, Or extracts of Oriental japonica, Ormea extract, Omija extract, Mukmun dong extract, Arun extract, Sculpture extract, Grape blood extract and Mycorrhizae extract have synergistic biological activities.

In the present invention, the term "extract" is intended to mean an extract obtained by the above-mentioned extraction treatment, a diluted or concentrated liquid of the extracted liquid, a dried material obtained by drying the extracted liquid, a controlled preparation or a purified product of the extracted liquid, And extracts of all formulations which can be formed using extracts.

Each of the above-mentioned extracts describes each plant and may include leaves, stems, bark, roots, flowers or flowers, fruit, seeds, sap, and whole plants in addition to the specified parts.

In order to prepare the above extract, one of ordinary skill in the art can use any suitable method known in the art. For example, a solvent extraction method can be used. The entire plant or any portion thereof may be ground (e.g., a blender) and then the extraction solvent may be treated to obtain a solvent extract. It may be subjected to a drying process (for example, drying at 40 to 70 DEG C for 15 to 50 hours) before pulverization and then pulverization. In addition, the solvent extract may be prepared in powder form by an additional process such as vacuum distillation and freeze-drying or spray-drying.

The kind of the extraction solvent used is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or mixtures thereof. Water and lower alcohols may be used alone or in combination of two or more. The solvent extract may be prepared by extracting the extract at least one time using the solvent, and the dry extract obtained by vacuum distillation or spray drying the solvent extract may be prepared.

The amount of the extraction solvent may vary depending on the kind of the extraction solvent used, but may be, for example, 1 to 20 times, or 5 to 20 times the dry weight of the target plant.

In addition, various extraction processes known in the art such as, for example, maceration, infusion, percolation, digestion, decoction, hot continuous extraction, aqueous- (For example, hydrofluoro-carbon solvent), etc.), which may be used alone or in combination with one another, may be used, for example, May be carried out by using two or more methods in combination.

[Composition]

The composition according to the present invention can be used as an extract of Oriental jelly, Omega extract, Omiza extract, Mukmun dong extract, Asuka extract, Sculpture extract, Or extracts such as raw persimmon extracts, omega extracts, omija extracts, mukmun dong extracts, oblong extracts, sculptor extracts, blood extracts, and tobacco extracts.

In the present invention, the term "included as an active ingredient" means that the extract is added to the skin composition of the present invention to such an extent that it can exhibit a skin improving effect, and various components are added as a sub ingredient And it is possible to formulate in various forms.

Each plant extract may be included in the composition according to the present invention in the following proportions. For example, the weight ratio of the extracts such as the raw persimmon extract, the omega extract, the omija extract, the mungum dong extract, the oblong extract, the sculptor extract and the blood extract is 1: 0.01-10: 0.01-10: 0.01-10: 0.01-10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10, 1: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 To 5: 1 to 5 or 1: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3.

In addition, the weight ratio of the raw persimmon extract, the omega extract, the omija extract, the mungmundong extract, the extract, the extract of the sculptor, the extract of the grapevine and the extract of the tobacco extract is 1: 0.01-10: 0.01-10: 0.01-10: 0.01-10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10, or 1: 1 to 5: 1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 5: 1 to 5: 1 to 5: 3 < / RTI >

The composition according to the present invention may contain at least about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005%, 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010% 0.0021%, 0.002%, 0.002%, 0.002%, 0.0024%, 0.0025%, 0.002%, 0.002% , 0.0028%, 0.0029%, 0.0030%, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0038%, 0.0039%, 0.0040%, 0.0041%, 0.0042% %, 0.0045%, 0.0046%, 0.0047%, 0.0048%, 0.0049%, 0.0050%, 0.0051%, 0.0052%, 0.0053%, 0.0054%, 0.0055%, 0.0056%, 0.0057%, 0.0058%, 0.0059% 0.0071%, 0.007%, 0.007%, 0.007%, 0.007%, 0.005%, 0.006%, 0.007% , 0.0078%, 0.0079%, 0.0080%, 0.0081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.0088%, 0.0089%, 0.0090%, 0.0091%, 0.0092% %, 0.0095%, 0.0096%, 0.0097%, 0.0 0.025%, 0.0350%, 0.0375%, 0.0400%, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.005%, 0.08%, 0.0099%, 0.0100%, 0.0200%, 0.0250%, 0.0275%, 0.0300% , 0.075%, 0.0600%, 0.0625%, 0.0650%, 0.0675%, 0.0700%, 0.0725%, 0.0750%, 0.0775%, 0.0800%, 0.0825%, 0.0850%, 0.0875%, 0.0900%, 0.0925%, 0.0950% %, 0.1000, 0.1250, 0.1500, 0.1750, 0.2000, 0.2250, 0.2500, 0.2750, 0.3000, 0.3250, 0.3500, 0.3750, 0.4000, 0.4250, 0.4500, 0.4750, 0.5000, 0.5250, 0.550, 0.5750, 0.6000, 0.6250, 0.6500, 0.6750, 0.7000, 0.7250, 0.7500, 0.7750, 0.8000, 0.8250, 0.8500, 0.8750, , 0.9200%, 0.9500%, 0.9750%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2% , 2.4%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7% 4.5%, 4.1%, 4.2%, 4.3%, 4.4%, 4.5%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5% , 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7.0%, 7.1% 8.0%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 7%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.8%, 7.9% , 8.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14% %, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29% The composition may comprise one or more extracts of the present invention in the range of 40%, 45%, 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% or 99%

The composition according to the present invention may also contain at least about 0.0001% to 90%, 0.001% to 90%, 0.01% to 90%, 0.1% to 90%, 0.1% to 90% %, 0.1 to 80%, 0.1 to 70%, 0.1 to 60%, 0.1 to 50%, 0.1 to 40%, 0.1 to 30%, 0.1 to 20% or 0.1 to 10% . ≪ / RTI >

The% can be calculated on the basis of the total weight of the composition or the volume relative to the total volume, and the concentration can be adjusted according to the desired effect of the composition or the product into which the composition is incorporated.

The compositions of the present invention may be formulated into any type of vehicle. Examples of suitable vehicles include, but are not limited to, emulsions (e.g., oil, water, water in silicone, water in silicone, water in heavy oil, water in oil, water in heavy water, But are not limited to, a hydro-alcoholic solution), a dry basis (e.g., lipstick and powder), a gel, ointment, paste, milk, liquid, aerosol, solid form or eye jelly.

The compositions of the present invention may also be encapsulated for delivery to a target area, such as the skin. Encapsulation techniques include, for example, the use of liposomes, follicles, and / or nanoparticles (e. G., Components that are trapped, encapsulated, and / or absorbed polymeric materials that can be used as a delivery vehicle But are not limited to, the use of biodegradable and non-biodegradable colloidal particles, including, for example, nanospheres and nanocapsules.

The composition according to the present invention can be variously commercialized. For example, cosmetic compositions, food compositions, and the like.

The cosmetic composition may be prepared, for example, in the form of a general emulsified formulation and a solubilized formulation. For example, creams, essences, serums, cosmetic ointments, sprays, oil gels, gels such as lotions such as lotion, facial lotion, body lotion and the like such as flexible lotion or nutrition lotion, nutrition cream, A lotion, a cleansing lotion, a makeup remover such as a cleansing oil, a cleansing foam, a soap, a body wash and the like such as a foundation, a pack, a sunscreen, a makeup base, a liquid type, a solid type or a spray type, But may be formulated into various forms known in the art without limitation.

In addition to the extract according to the present invention, the above-mentioned cosmetic composition may further include optional ingredients commonly known as ingredients of the cosmetic composition in the art, so long as the object of the present invention is not impaired. Emulsifying agents, stabilizers, lubricants, solvents, moisturizers (e.g. emollients, humectants, film forming agents, occlusive agents, emulsifiers, water-repellant, a UV absorber (including physical and chemical absorbents such as para-aminobenzoic acid ("PABA") and corresponding (Eg, PABA derivatives, titanium dioxide, zinc oxide, etc.), essential oils, vitamins such as A, B, C, D, E and K, trace metals such as zinc, calcium and selenium, Antioxidants (such as BHT and tocopherol), chelating agents (such as disodium EDTA and tetrasodium EDTA), antioxidants (for example, antimicrobial agents) , Preservatives (such as methylparaben and propylparaben), pH adjusting agents (such as sodium hydroxide and citric acid) (E.g., aluminum starch octenyl succinate, kaolin, corn starch, oat starch, cyclodextrin, talc, and zeolites), skin bleaching and lightening agents (e.g., Hydroquinone and beta-hydroxynaphthoic acid (e.g., hydroquinone and niacinamide lactate), wetting agents such as glycerin, propylene glycol, butylene glycol, pentylene glycol, sorbitol, urea and mannitol, exfoliants (E.g., magnesium / aluminum hydroxide stearate), skin conditioning agents (e.g., aloe extracts), and the like, , Allantoin, bisabolol, ceramide, dimethicone, hyaluronic acid, and dipotassium glycyrrhizate), and thickening agents (e.g., (E.g., silicone oils and polyorganosiloxanes), and the like, which are capable of increasing viscosity, such as carboxylic acid polymers, crosslinked polyacrylate polymers, polyacrylamide polymers, polysaccharides and gums, .

The food composition may be formulated into, for example, beverages, fortified water, energy drinks, nutritional drinks, solid foods, vitamins, supplements, etc., But is not limited thereto. Such adjuvants may include vitamins, minerals, herbs or other plants, amino acids, enzymes and metabolites. Such adjuvants are suitable for oral consumption and may be administered orally.

May be provided as a kit comprising the composition according to the present invention. The composition according to the invention is contained in a container which can contain a bottle, a metal tube, a laminate tube, a plastic tube, a dispenser, a pressure vessel, a barrier container, a package, a compartment, a lipstick container, a compact container, A cosmetic pan, or other type of container, including, but not limited to, a plastic container that is injected or blow-molded into a suitable bottle, dispenser, or package to be maintained or dispersed, . The kit may include an instruction for using the kit or composition, the instruction sheet may be described on a separate sheet, or may be described on the surface of the container surface, the surface of the container wrapper. Instructions include, but are not limited to, letters, phrases, abbreviations, pictures or symbols. The instructions may include, for example, instructions on how to use, apply and maintain the kit or composition. The container can be dispensed according to a predetermined amount.

The composition according to the present invention may be provided as a topical skin composition.

In addition, a method of topically applying the composition according to the present invention to the skin can be provided.

In the present invention, the term "topical application" means applying or applying the composition on the surface of keratinous tissue, and "topical skin composition" includes compositions suitable for topical application or application on keratinocytes. Such compositions are typically dermatologically acceptable in that they do not have excessive toxicity, incompatibility, instability, allergic response, etc. when applied to the skin. The topical skin care compositions of the present invention may have a selected viscosity to avoid significant dripping or pooling after application to the skin.

[Skin improvement effect]

The composition according to the present invention has biological activity and exerts excellent effects on skin improvement.

More specifically, the composition according to the present invention has a skin whitening effect.

The skin whitening refers to inhibiting melanin formation and inhibiting melanin formation.

As a result of measuring the amount of melanin produced by treating the melanoma cells according to the present invention, the inventors of the present invention confirmed that the melanin production inhibitory effect was enhanced compared with the case of treating each extract, It was found that the effect exerted an effect corresponding to known arbutin. It was confirmed that the composition according to the present invention was excellent in the effect of inhibiting melanin formation and exhibited excellent whitening effect.

In addition, the composition according to the present invention has an effect of improving wrinkles and elasticity.

The skin wrinkles are caused by the skin of the skin and can be caused by the cause of the gene, collagen and elastin present in the skin dermis, and the external environment.

The wrinkle improvement refers to suppressing or inhibiting the generation of wrinkles, or alleviating the already generated wrinkles.

The skin elasticity refers to elastic fibers composed of elastin existing in the dermal layer and collagen fibers called collagen. The elasticity improvement refers to suppressing or inhibiting elasticity reduction or maintaining or improving elasticity .

As a result of treating the complex extract according to the present invention, the present inventors confirmed that they exhibited an enhanced collagen synthesis promoting effect and an elastase activity inhibiting effect, respectively, as compared with the case of treating each extract, And the effect of inhibiting the activity of skin elastase was shown to exert an effect corresponding to the known quercetin. The composition according to the present invention is excellent in collagen synthesis promoting effect and elastase activity inhibiting effect and shows excellent wrinkles and elasticity improving effect.

Further, the composition according to the present invention has an effect of improving the trouble.

The above-mentioned troubles are symptoms such as skin irritation and inflammation. The inflammatory reaction is characterized by pain, fever, redness, swelling and malfunction due to external stimuli. Histologically, the permeability of the arterioles, capillaries, , Complicated symptoms including enlargement accompanied by increase, excretion of plasma containing plasma protein, migration to inflammation site of leukocyte, and skin irritation reaction are characterized by erythema, itching, fever and the like.

The improvement of the trouble refers to inhibiting or inhibiting the skin irritation reaction and the inflammation reaction, or alleviating the skin irritation reaction or the inflammation reaction already in progress.

As a result of measuring the inhibitory effect on NO production by treating the compound extract according to the present invention, the present inventors confirmed that the inhibitory effect on NO production was enhanced compared to the case of treating each extract, and NO inhibitory effect was known And exhibited an effect corresponding to that of L-NMMA. It was confirmed that the composition according to the present invention exhibits excellent skin trouble improving effect through inhibition of NO production.

In addition, the composition according to the present invention has an antioxidative effect.

The antioxidant refers to inhibition of cellular oxidation by free radicals or reactive oxygen species (ROS), which are highly reactive according to oxidative stress caused by intracellular metabolism or ultraviolet rays, and free radicals Or removal of reactive oxygen species, thereby reducing damage to the cells.

As a result of measuring the free radical scavenging ability of the complex extract according to the present invention, the inventors of the present invention confirmed that they exhibited an increased free radical scavenging ability as compared with the case of treating each extract, and found that the free radical scavenging ability of vitamin C And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits an excellent antioxidative effect.

In addition, the composition according to the present invention has an antiherating effect.

The glycation refers to a phenomenon in which a sugar and a protein are bound to each other due to the presence of excessive sugars and the protein is destroyed. As glycosylation progresses, it affects collagen and elastin, resulting in skin elasticity deterioration and other skin damage.

As a result of measuring the antagonism effect by treating the complex extract according to the present invention, the inventors of the present invention have confirmed that they exhibit an elevated antihyperglycosylation effect as compared with the case of treating the respective extracts, and the aminoguanidine And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits excellent anticarcinogenic effect.

In addition, the composition according to the present invention has a fibroblast proliferation effect.

Among the compositions according to the present invention, it is an effect which occurs in a composition including both 'persimmon extract, omega extract, omija extract, mukmun dong extract, oblong extract, sculpture extract, blood extract, and toad extract.

The composition according to the present invention may be used for fibroblast proliferation in vitro and / or in vivo.

As a result of measuring the cell proliferation rate by treating the complex extract according to the present invention, the present inventors have confirmed that the extract according to the present invention exhibits an increased cell proliferation effect as compared with the case of treating each extract, Proliferative effect was confirmed.

The complex extract according to the present invention is safe for the skin and has an excellent effect for improving the skin including anti-glycation effect, skin whitening effect, skin elasticity and wrinkle improving effect, antioxidative effect and skin trouble improving effect. It also has a fibroblast proliferation effect.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the embodiments and experimental examples according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the above-described embodiments and experiments. The embodiments and experimental examples of the present invention are provided to enable those skilled in the art to more fully understand the present invention.

≪ Example 1 > Preparation of fresh persimmon extract

After the fresh persimmon was cut three times, 100 g of the dry weight was put into a flask, and the mixture was extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The frozen extract was filtered with a filter having a pore size of 0.2 탆 to prepare a raw persimmon extract.

Example 2: Preparation of oats extract

The oats were well dried and cut into small pieces. 100 g of dry weight was placed in a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare an oat extract.

Example 3: Preparation of Omija extract

The omigas were well dried, and then placed in a flask with a dry weight of 100 g and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare an Omija extract.

<Example 4> Preparation of extract

The outlets were well dried and cut into three pieces. 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare an extract.

<Example 5> Preparation of extract

After drying thoroughly, 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a mulberry extract.

Example 6 Preparation of a Sculptor Extract

The sculptor was dried well, and the dried weight of 100 g was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a sculptor extract.

<Example 7> Preparation of extracts

Briefly, the blood was removed by drying and then 100 g of dry weight was added to the flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare an extract of a blood-brain extract.

Example 8 Preparation of Toyocho extract

The tobacco was dried well and three times. The dried weight of 100 g was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a tobacco extract.

<Example 9> Preparation of mixed extracts of raw persimmon, osseum, omija, mangmundong, oboe, sculptor,

As in the above-mentioned examples, the same extractives were prepared by injecting the same amount of raw materials such as raw leaves, oats, omija, mokmundong, broth, sculptor, and blood, which were prepared in the form of extracts, respectively, to produce mixed extracts such as scallions, omies, .

<Example 10> Preparation of mixed extract of Toyocho, Oume, Omija, Maekmundong, Arup, Sculptor,

In the same manner as in the above-mentioned embodiments, the same amount of the bean jam, which is prepared in the form of an extract, is injected into the bean jam, such as Jijiji, Omachi, Omija, Maekmundong, Mixed extracts were prepared.

&Lt; Experimental Example 1 >

In order to confirm anti-glycation efficacy, L-arginine and glucose were used to measure glycosylation-inhibiting activity.

First, 1M L-arginine and 1M glucose were dissolved by using 1M phosphate buffer (pH 7.4), and prepared by diluting the sample to 50ppm with 1M phosphate buffer solution. 1M L-arginine and 1M phosphate buffer solution were mixed at a ratio of 1: 4, and then 80 [mu] l each was added to a 96-well plate. To each sample, 100 μl of 0.01 M aminoguanidine was added to each sample to be diluted to 50 ppm and used as a positive control. These samples were mixed well and finally glucose diluted with 1M phosphate buffer solution was added to the final concentration of glucose to 0.1 M and reacted at 70 ° C for 4 hours. The degree of saccharification was measured by measuring the absorbance of the 96-well plate at 420 nm using a spectrophotometer.

Glycation group of the following formula was an experimental group in which 1M L-arginine and 1M glucose were added to induce glycation. Absorbance was measured at 420 nm by adding 1M L-arginine and sample alone without glucose to measure the absorbance of the sample itself. The saccharide inhibitory activity can be obtained by the following formula.

 [Equation 1]

('Glycation test group' in the above equation (1) means 'Absorbance of Glycation test group').

As shown in the results of Table 1 above, it can be seen that the mixed extract is superior to the aminoguanidine, which is known as an anticarious substance, in the anticarcinogenic effect.

<Experimental Example 2> Effect of inhibiting melanin formation

In order to confirm the whitening effect through inhibition of melanin formation, a culture solution of B-16 mouse melanoma cells in rat was cultured according to the method described in Lotan R. et al. (Cancer Res. 40: 3345-3350, 1980) The total amount of melanin was measured by adding an extract. In the experiment, first the toxicity of melanoma cells in rats was evaluated and the whitening evaluation was carried out at a concentration that is not toxic. DMSO was used as a negative control group, and albutin was used as a positive control group.

Specifically, the sample was added to the medium to a final concentration of 100 ppm, arbutin was added to the medium to be 100 ppm, and melanoma cells were cultured for 3 days. Cells were then trypsinized, detached from the culture, centrifuged, and then melanin was extracted. The removed cells were incubated with 1 ml of sodium hydroxide solution (1N concentration), boiled for 10 minutes to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.

The amount of melanin was measured by an absorbance of 1 × 10 ⁶ cells per unit cell, and the total amount of melanin relative to the control group was calculated as the inhibition rate (%). The results are shown in Table 2 below. As shown in FIG.

As can be seen from the results of Table 2 above, the mixed extract showed excellent melanin total amount reduction effect and was found to be useful for whitening purposes.

<Experimental Example 3> Anti-inflammatory effect

In order to confirm the anti-inflammatory effect and the improvement of skin trouble, nitric oxide (NO) production inhibition experiment was performed by GRIESS method using RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, macrophages of mice, were subcultured several times, placed in 24-well plates so as to enter 3x10 ⁵ wells into each well, and cultured for 24 hours. Subsequently, the cell culture medium containing the sample was replaced with a final concentration of 10 ppm. At this time, L-NMMA (L-NG-Monomethylarginine), which is an inhibitor of NO production, was treated together as a positive control and cultured for 30 minutes. Lipopolysaccharide (LPS) 100 μl of the supernatant was transferred to a 96-well plate, 100 μl of GRIESS solution was added thereto, and the reaction was allowed to proceed at room temperature for 10 minutes. The absorbance at 540 nm was measured to determine the NO inhibitory effect. Calculated using Equation (2) and shown in Table 3 below. Experiments were performed three times each and expressed as average values.

&Quot; (2) &quot;

NO production inhibition rate (%) = {(absorbance of negative control - absorbance of each extract) / absorbance of negative control} x 100

As can be seen from the results of Table 3, it was found that the mixed extracts exhibited excellent activity as a natural substance when compared with the representative anti-inflammatory drug L-NMMA, although the relative activity was somewhat low.

<Experimental Example 4> Promoting collagen synthesis

The sample was added to the culture medium of human - derived fibroblasts to confirm the promoting effect of type I collagen synthesis at the cellular level. The synthesized collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay Kit). In order to measure the amount of collagen synthesis, the sample was added to a fibroblast culture medium (DMEM medium) at a final concentration of 10 ppm and cultured for 48 hours. The culture broth was taken and the degree of type 1 collagen synthesis was measured at each concentration using a PICP EIA kit And measured at 450 nm using a spectrophotometer.

For the comparison of the effects, the degree of collagen synthesis was measured in the same manner for the samples in which the culture medium of the untreated fibroblasts (negative control) and vitamin C (positive control) were added to a final concentration of 52.85 / / ml. The increase rate of collagen production was calculated by the ratio of relative collagen production to the negative control, and the results are shown in Table 4 below.

As can be seen from the results in Table 4, the collagen synthesis was increased when the mixed extract was treated, and the collagen synthesis effect was comparable to that of vitamin C, which is generally known to induce collagen synthesis.

<Experimental Example 5> Elastase activity inhibitory effect

The activity inhibitory effect of Elastase, an enzyme that degrades elastin, was confirmed as follows.

Elastase used Elastase from human leukocyte cells and MeOSuc-Ala-Ala-Pro-Val-pNA as synthetic substrate for Elastase. The buffer solution used was 100 mM Tris (pH 7.5) solution. Finally, 0.2 mU was used for the ELASTASES using a buffer solution. In addition, the synthetic substrate of Elastase was diluted with buffer solution to make a final concentration of 0.5 mM by making a 100 mM solution using DMSO. At this time, the positive control group was set to contain 10 ppm of quercetin, which is known as an inhibitor of Elastinase. Ellazease inhibition candidates were added to give a final concentration of 10 ppm. The reaction was carried out in a 96-well plate and allowed to react at room temperature for 20 minutes. Absorbance was measured at 405 nm using a spectrophotometer at intervals of 1 minute, and the slope of the absorbance versus time was determined as the activity of the enzyme. Ella stasis inhibition rates were calculated as follows.

&Quot; (3) &quot;

The inhibition rate of elastase was calculated using Equation (3) and shown in Table 5 below.

As can be seen from the results in Table 5, when the mixed extract was treated, it was found that it exhibited a better inhibitory effect on elastase activity, though the effect was somewhat weaker than that of the positive control. Therefore, it was found that the mixed extract can be used for skin regeneration and wrinkle improvement.

<Experimental Example 6> Antioxidative effect

The free radical scavenging ability of the mixed extract was measured by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) method (Blois, Nature 181, 1190, 1958). DPPH is a relatively stable free radical, which shows maximum absorption at 517 nm in the presence of radicals and loses its absorbance when radicals are eliminated. DPPH was purchased from Sigma, and dissolved in methyl alcohol at a concentration of 0.15 mM.

First, 100 μl of mixed extract or vitamin C, a positive control, was added to each well of a 96-well plate. 100 μl of DPPH solution was added thereto, and the mixture was allowed to stand at room temperature for 30 minutes, and the absorbance at 517 nm was measured using a microplate reader (BioTek EL-340).

IC ₅₀ represents the concentration of the extract when the absorbance of the sample is half the absorbance of the control group. The results are shown in Table 6 below. This experiment was repeated 3 times.

As shown in Table 6, the mixed extracts showed a strong free radical scavenging ability as compared with vitamin C, confirming that the antioxidative effect was excellent.

<Experimental Example 7> Cell proliferation effect

The cell proliferation effect was examined by the following method. Human fibroblast cells were inoculated into a 96-well plate (Corning) and incubated for 2 days at a concentration of 1% in Examples 1 to 8, followed by MTT assay Cell proliferation was evaluated.

More specifically, human fibroblasts were counted by a hemocytometer in a 24-well plate at a density of 5 × 10 ³ cells / well, and then divided. After culturing in DMEM containing 10% FBS for 48 hours and culturing for 40-50% of the culture vessel surface area, the sample was replaced with FBS-free DMEM and further cultured for 24 hours. After incubation, 50 μl of a solution of 2.5 mg / ml 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT, Sigma M5655, USA) was added and cultured for additional 3 hours. Then, 200 μl of dimethyl sulfoxide (DMSO, Sigma D2650, USA) was added to each well and the mixture was treated with 200 μl of each solution. 100 μl of each well was taken in 96 wells and subjected to Enzyme-Linked Immunosorbent Assay (ELISA) Absorbance was measured at 570 nm. As a control group, serum-free medium was used, and 1% serum was added as a comparative group. The experiment was repeated three times each, and the results are shown in Table 7.

As shown in the results of Table 7, it was found that the mixed extract according to the present invention is excellent in cell activation effect through cell proliferation. In addition, it was found that Example 10 in which the mixed extract of Example 9 was mixed with the extract of Mycorrhizal fungus showed an additional synergistic effect in terms of cell proliferation.

Claims (6)

A cosmetic composition for skin whitening, wrinkle, elasticity, trouble, or antioxidation, which comprises an extract of Sejungburo extract, Omega extract, Omija extract, Mukmun dong extract, Arun extract, The method according to claim 1, wherein the weight ratio of the raw persimmon extract, the omega extract, the omija extract, the mungmundong extract, the oblong extract, the scarlet extract and the blood extract is 1: 0.01-10: 0.01-10: 0.01-10: 0.01-10: 10: 0.01 ~ 10. A cosmetic composition for the proliferation of fibroblasts, which comprises as an active ingredient an extract of Rhodiola citrius, an extract of Omega, an extract of Omija, a extract of Macmundan, an extract, an extract of Sculptor, 4. The method according to claim 3, wherein the weight ratio of the raw persimmon extract, the omega extract, the omija extract, the mungum dong extract, the oblong extract, the sculpture extract, the blood extract and the tobacco extract is 1: 0.01-10: 0.01-10: 0.01-10: 10: 0.01-10: 0.01-10: 0.01-10. The cosmetic composition according to claim 1 or 3, wherein the extract is extracted with a solvent selected from the group consisting of water, C1 to C4 lower alcohols, and mixtures thereof. The cosmetic composition according to claim 1, wherein the cosmetic composition is antialceration, inhibition of melanin formation, promotion of collagen synthesis, inhibition of elastase activity or free radical scavenging.