KR20170136912A - Composition for improving skin condition comprising herb extracts mixture - Google Patents

Abstract

The present invention relates to a composition containing as an active ingredient a complex herbal extract which is safe when applied to skin and has excellent skin improving effect. More specifically, the composition according to the present invention is a composition comprising, as an active ingredient, a medicinal herb extract, a corn oil extract, a medicinal extract, an angelica extract, a persimmon extract, a mungumodong extract, a herb extract, It has an excellent effect in skin improvement including effects, skin whitening effect, skin elasticity and wrinkle improving effect, antioxidative effect and skin trouble improving effect.

Description

[0001] The present invention relates to a composition for improving skin comprising a herbal extract,

The present invention relates to a composition containing as an active ingredient a complex herbal extract which is safe when applied to skin and has excellent skin improving effect.

The skin is an important body that is responsible for various physiological functions such as barrier function, body temperature control function, excretion function, which protects the human body from the external environment and prevents the internal moisture and useful components from flowing out. However, due to the following reasons, the activity of skin cells may deteriorate and the skin condition may deteriorate.

Collagen and elastin present in the dermis of the skin perform functions such as mechanical rigidity of the skin, resistance of the connective tissue, maintenance of the cohesion of the tissue, and support of the cell adhesion, and have a great influence on the constituents of the skin. It is known that such collagen is reduced by photoaging due to stress, aging, or ultraviolet irradiation, while elastin is known to be distorted in three-dimensional structure by the enzymatic degradation enzyme Elastase after exposure to ultraviolet light. As a result, the skin tissue is loosened and the elasticity is lost.

When the cell function of the epidermis is lowered, the metabolism is not smooth and the exfoliation does not occur well, and when the ultraviolet rays are received in a state where the keratin is overly accumulated, the elasticity is lowered and wrinkles are generated. If the skin is not formed on the epidermis, moisture and sebum secretion are reduced, and the skin becomes dry and wrinkles are formed.

When the skin receives ultraviolet rays, melanin is synthesized to protect the skin. The synthesized melanin is transferred to the keratinocytes of the skin through the melanoma. This keratinocyte turns over over a period of 28 days. Therefore, the melanin produced is generally lost by the keratinocyte periodically for 28 days. However, when the skin cell cycle is not properly controlled by the stress, skin aging, etc., keratinocytes do not fall out in the presentation period and the pigment such as spots, freckles, Calm occurs.

In the skin, sebum, sweat and cosmetic ingredients are decomposed into substances that are toxic to the skin by the fungus, which can cause skin irritation and inflammation. In addition, skin irritation caused by ultraviolet rays increases the production of nitrogen monoxide (NO), which is an inflammation mediator, to cause skin troubles. The production of nitrogen monoxide is mostly caused by iNOS, and it is known that iNOS is rapidly induced by stimulation such as LPS and cytokine to produce excessive NO.

A reactive oxygen species (ROS), also known as a toxic oxygen species, is a cell-generated toxic substance produced by physiological actions such as respiration and is constantly produced and extinguished, with 3-5% in normal conditions . These reactive oxygen species are free radicals such as superoxide radicals (O2-) and hydroxyl radicals (HO +), which are chemically bound to atoms or molecules that are not paired with the outermost electron orbits Highly unstable and highly reactive) or in the form of compounds with paired electrons such as hydrogen peroxide (H ₂ O ₂ ) or singlet radicals. Active oxygen species have the advantage of biologically protecting bacteria in the physiological system, but generally cause oxidation in vivo, causing harmful effects that cause disease. It has been reported that these reactive oxygen species attack biological molecules and damage cells and tissues and cause various diseases related to aging and various diseases.

Research has been continued to suppress the skin aging caused by various factors as described above, to improve whitening, wrinkles, elasticity or trouble, and to develop a substance having an antioxidative effect.

Materials having the above-mentioned effects are widely distributed in the natural world, and they have been mainly used as raw materials for foods, cosmetics, medicines and the like using materials derived from plants. However, the substances derived from such natural substances are not effective enough to be used in large quantities in order to obtain a meaningful effect, resulting in toxicity and price increase.

In order to solve the problems of such natural materials, the development of chemically synthesized materials has continued. Although they have a merit that they exert a superior effect even when used in a small amount compared to a natural substance, their use is limited due to a fatal problem that can cause large and small side effects to the human body.

Accordingly, it is inevitable to develop a substance derived from a natural product and having stability, while exhibiting an excellent effect on skin improvement.

KR 1020060092179 A KR 1020090002682 A

A problem to be solved by the present invention is to provide a complex herbal medicine extract which is derived from natural materials and has stability, while exhibiting excellent effects on skin, in particular whitening, wrinkle, elasticity, trouble improvement and antioxidation.

In order to solve the above problems, the present invention provides a skin whitening and wrinkle-improving agent which comprises, as an active ingredient, a medicinal extract, a marine oil extract, a moxibustion extract, a mossy moss extract, a mossy moss extract, a mussel moss extract, a mossy moss extract, , Elasticity improvement, trouble-improvement, or antioxidation (for example, a cosmetic composition).

[extract]

熟地 黄 (熟地黄) is the root of the scrophulariaceae (Rehmannia glutinosa (Gaertner) Liboschitz ex Steudel) is steamed and dried, darkens the hair color and is said to be effective in the recovery of fatigue.

Cornus (山茱萸) is a tree of Cornus cornaceae (Cornaceae) (Cornus officinalis Siebold et Zuccarini). It is known that it has the effects of strengthening (阴 阴), strengthening of the kidney (肾精) and 肾气 (肾气), and convergence.

Yamagishi is steamed and dried as a rootstock (dipseye ) from which the jam of Dioscoreaceae ( Dioscorea japonica ) or horse (Disocorea batata s) is removed.

Hwanggi (皇 耆) is the legume of the leguminosae ( Astragalus) membranaceus Bunge) or Mongolian shaggy (蒙古 黄耆, Astragalus There are membranaceus Bunge. mongholicus Hsiao), or as a root. Hwanggi is mild and mild.

Rehmanniae Radix Recens is a scrophulariaceae and is a fresh root of Rehmannia glutinosa Liboschitz ex Steudel. Its taste is slightly worn, its quality is very cold.

Macmun-dong is a perennial herbaceous plant belonging to the Liliaceae. It is the bulge of the Liriope platyphylla Wang et Tang or Ophiopogon japonicus Ker-Gawler root.

Dendrobium nobile Lindley, Orchidaceae, Dendrobium nobile Lindley, Dendrobium, loddigesii Rolfe, Dendrobium fimbriatum Hook var oculatum Hook, Dendrobium chrysanthum Wall ex Lindley or Dendrobium candidum Wall ex Lindley stem to be. Grain is almost odorless, flavor is sweet and quality is slightly cold.

The gymnosperm is an anemarrhena belonging to the Liliaceae asphodeloides Bunge) is the roots of. There is a slight peculiar odor, and the taste is worn and temperate.

Hwangwa (黄柏) is a rudaceae (Rutaceae) belonging to the primate ( Phellodendron amurense Ruprecht) or yellow flower (黄皮 樹, Phellodendron chinense Schneider). Hwang Baek wears a flavor and the quality is cold.

It is the beginning of the first year of the Labiatae and is the outpost of the genus Schizonepeta tenuifolia Briquet.

The present inventors have found that all of the above extracts have a biological activity beneficial to the skin, and the complex extracts of two or more of the above extracts, preferably a medicinal extract, a mushroom extract, a mushroom extract, a mushroom extract, a mushroom extract, It was found that the combined extracts of Huang Baek extract and mold extract had synergistic biological activity.

In the present invention, the term "extract" is intended to mean an extract obtained by the above-mentioned extraction treatment, a diluted or concentrated liquid of the extracted liquid, a dried material obtained by drying the extracted liquid, a controlled preparation or a purified product of the extracted liquid, And extracts of all formulations which can be formed using extracts.

Each of the above-mentioned extracts describes each plant and may include leaves, stems, bark, roots, flowers or flowers, fruit, seeds, sap, and whole plants in addition to the specified parts.

In order to prepare the above extract, one of ordinary skill in the art can use any suitable method known in the art. For example, a solvent extraction method can be used. The entire plant or any portion thereof may be ground (e.g., a blender) and then the extraction solvent may be treated to obtain a solvent extract. It may be subjected to a drying process (for example, drying at 40 to 70 DEG C for 15 to 50 hours) before pulverization and then pulverization. In addition, the solvent extract may be prepared in powder form by an additional process such as vacuum distillation and freeze-drying or spray-drying.

The kind of the extraction solvent used is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or mixtures thereof. Water and lower alcohols may be used alone or in combination of two or more. The solvent extract may be prepared by extracting the extract at least one time using the solvent, and the dry extract obtained by vacuum distillation or spray drying the solvent extract may be prepared.

The amount of the extraction solvent may vary depending on the kind of the extraction solvent used, but may be, for example, 1 to 20 times, or 5 to 20 times the dry weight of the target plant.

In addition, various extraction processes known in the art such as, for example, maceration, infusion, percolation, digestion, decoction, hot continuous extraction, aqueous- (For example, hydrofluoro-carbon solvent), etc.), which may be used alone or in combination with one another, may be used, for example, May be carried out by using two or more methods in combination.

[Composition]

The composition according to the present invention contains, as an active ingredient, a medicinal extract, a corn oil extract, a medicinal extract, a medicinal herb extract, a persimmon extract, a mungmun dong extract, a herb extract, a gum extract, a yellowish white extract and a mold extract.

In the present invention, the term "included as an active ingredient" means that the extract is added to the skin composition of the present invention to such an extent that it can exhibit a skin improving effect, and various components are added as a sub ingredient And it is possible to formulate in various forms.

Each plant extract may be included in the composition according to the present invention in the following proportions. For example, the weight ratio of extract of Sukjwanghwang Extract: Sangwoohwang Extract: Chrysanthemum Extract: 1: 0.1 ~ 10: 0.1 ~ 10: 0.1 ~ 10: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 5: 0.1 to 5: 0.1 to 5: 0.1 to 5, 1: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 3: 0.1 to 2: 0.1 to 2: 0.1 to 2: 0.1 to 2, 1: 0.5 to 1.5: 0.5 to 2: 0.1 to 2: 0.1 to 2: 0.1 to 2: 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5: 0.5 to 1.5.

The compositions according to the present invention may contain at least about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005%, 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010%, 0.0011%, 0.0012% , 0.0014%, 0.0015%, 0.0016%, 0.0017%, 0.0018%, 0.0019%, 0.0020%, 0.0021%, 0.0022%, 0.0023%, 0.0024%, 0.0025%, 0.0026% %, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0038%, 0.0039%, 0.0040%, 0.0041%, 0.0042% 0.005%, 0.0057%, 0.0058%, 0.0059%, 0.0060%, 0.0061%, 0.0062%, 0.0063%, 0.0058% , 0.0064%, 0.0065%, 0.0066%, 0.0067%, 0.0068%, 0.0069%, 0.0070%, 0.0071%, 0.0072%, 0.0073%, 0.0074%, 0.0075%, 0.0076%, 0.0077% %, 0.0081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.0088%, 0.0089%, 0.0090%, 0.0091%, 0.0092%, 0.0093%, 0.0094%, 0.0095% 0.0097%, 0.0098%, 0.0099%, 0.0100%, 0.0200%, 0.0250%, 0.0275 %, 0.0300%, 0.0325%, 0.0350%, 0.0375%, 0.0400%, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.0575%, 0.0600%, 0.0625%, 0.0650% 0.0725%, 0.0725%, 0.0750%, 0.0775%, 0.0800%, 0.0825%, 0.0850%, 0.0875%, 0.0900%, 0.0925%, 0.0950%, 0.0975%, 0.1000%, 0.1250%, 0.1500%, 0.1750%, 0.2000% , 0.2250, 0.2500, 0.2750, 0.3000, 0.3250, 0.3500, 0.3750, 0.4000, 0.4250, 0.4500, 0.4750, 0.5000, 0.5250, 0.550, 0.5750, 0.6000, 0.6250 %, 0.6500%, 0.6750%, 0.7000%, 0.7250%, 0.7500%, 0.7750%, 0.8000%, 0.8250%, 0.8500%, 0.8750%, 0.9000%, 0.9250%, 0.9500%, 0.9750%, 1.0% 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9% , 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0%, 4.1%, 4.2% 5.4%, 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2% 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7.0%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7% , 7.9%, 8. 9.2%, 9.4%, 9.4%, 9.5%, 9.6%, 8.3%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 9.0% , 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21% %, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 60%, 65%, 70%, 75% 85%, 90%, 95%, or 99% or more of the extract of the present invention. The% can be calculated on the basis of the total weight of the composition or the volume relative to the total volume, and the concentration can be adjusted according to the desired effect of the composition or the product into which the composition is incorporated.

The compositions of the present invention may be formulated into any type of vehicle. Examples of suitable vehicles include, but are not limited to, emulsions (e.g., oil, water, water in silicone, water in silicone, water in heavy oil, water in oil, water in heavy water, But are not limited to, a hydro-alcoholic solution), a dry basis (e.g., lipstick and powder), a gel, ointment, paste, milk, liquid, aerosol, solid form or eye jelly.

The compositions of the present invention may also be encapsulated for delivery to a target area, such as the skin. Encapsulation techniques include, for example, the use of liposomes, follicles, and / or nanoparticles (e. G., Components that are trapped, encapsulated, and / or absorbed polymeric materials that can be used as a delivery vehicle But are not limited to, the use of biodegradable and non-biodegradable colloidal particles, including, for example, nanospheres and nanocapsules.

The composition according to the present invention can be variously commercialized. For example, cosmetic compositions, food compositions, and the like.

The cosmetic composition may be prepared, for example, in the form of a general emulsified formulation and a solubilized formulation. For example, creams, essences, serums, cosmetic ointments, sprays, oil gels, gels such as lotions such as lotion, facial lotion, body lotion and the like such as flexible lotion or nutrition lotion, nutrition cream, A lotion, a cleansing lotion, a makeup remover such as a cleansing oil, a cleansing foam, a soap, a body wash and the like such as a foundation, a pack, a sunscreen, a makeup base, a liquid type, a solid type or a spray type, But may be formulated into various forms known in the art without limitation.

In addition to the extract according to the present invention, the above-mentioned cosmetic composition may further include optional ingredients commonly known as ingredients of the cosmetic composition in the art, so long as the object of the present invention is not impaired. Emulsifying agents, stabilizers, lubricants, solvents, moisturizers (e.g. emollients, humectants, film forming agents, occlusive agents, emulsifiers, water-repellant, a UV absorber (including physical and chemical absorbents such as para-aminobenzoic acid ("PABA") and corresponding (Eg, PABA derivatives, titanium dioxide, zinc oxide, etc.), essential oils, vitamins such as A, B, C, D, E and K, trace metals such as zinc, calcium and selenium, Antioxidants (such as BHT and tocopherol), chelating agents (such as disodium EDTA and tetrasodium EDTA), antioxidants (for example, antimicrobial agents) , Preservatives (such as methylparaben and propylparaben), pH adjusting agents (such as sodium hydroxide and citric acid) (E.g., aluminum starch octenyl succinate, kaolin, corn starch, oat starch, cyclodextrin, talc, and zeolites), skin bleaching and lightening agents (e.g., Hydroquinone and beta-hydroxynaphthoic acid (e.g., hydroquinone and niacinamide lactate), wetting agents such as glycerin, propylene glycol, butylene glycol, pentylene glycol, sorbitol, urea and mannitol, exfoliants (E.g., magnesium / aluminum hydroxide stearate), skin conditioning agents (e.g., aloe extracts), and the like, , Allantoin, bisabolol, ceramide, dimethicone, hyaluronic acid, and dipotassium glycyrrhizate), and thickening agents (e.g., (E.g., silicone oils and polyorganosiloxanes), and the like, which are capable of increasing viscosity, such as carboxylic acid polymers, crosslinked polyacrylate polymers, polyacrylamide polymers, polysaccharides and gums, .

The food composition may be formulated into, for example, beverages, fortified water, energy drinks, nutritional drinks, solid foods, vitamins, supplements, etc., But is not limited thereto. Such adjuvants may include vitamins, minerals, herbs or other plants, amino acids, enzymes and metabolites. Such adjuvants are suitable for oral consumption and may be administered orally.

May be provided as a kit comprising the composition according to the present invention. The composition according to the invention is contained in a container which can contain a bottle, a metal tube, a laminate tube, a plastic tube, a dispenser, a pressure vessel, a barrier container, a package, a compartment, a lipstick container, a compact container, A cosmetic pan, or other type of container, including, but not limited to, a plastic container that is injected or blow-molded into a suitable bottle, dispenser, or package to be maintained or dispersed, . The kit may include an instruction for using the kit or composition, the instruction sheet may be described on a separate sheet, or may be described on the surface of the container surface, the surface of the container wrapper. Instructions include, but are not limited to, letters, phrases, abbreviations, pictures or symbols. The instructions may include, for example, instructions on how to use, apply and maintain the kit or composition. The container can be dispensed according to a predetermined amount.

The composition according to the present invention may be provided as a topical skin composition.

In addition, a method of topically applying the composition according to the present invention to the skin can be provided.

In the present invention, the term "topical application" means applying or applying the composition on the surface of keratinous tissue, and "topical skin composition" includes compositions suitable for topical application or application on keratinocytes. Such compositions are typically dermatologically acceptable in that they do not have excessive toxicity, incompatibility, instability, allergic response, etc. when applied to the skin. The topical skin care compositions of the present invention may have a selected viscosity to avoid significant dripping or pooling after application to the skin.

[Skin improvement effect]

The composition according to the present invention has biological activity and exerts excellent effects on skin improvement.

More specifically, the composition according to the present invention has a skin whitening effect.

The skin whitening refers to inhibiting melanin formation and inhibiting melanin formation.

As a result of measuring the amount of melanin produced by treating the melanoma cells according to the present invention, the inventors of the present invention confirmed that the melanin production inhibitory effect was enhanced compared with the case of treating each extract, It was found that the effect exerted an effect corresponding to known arbutin. It was confirmed that the composition according to the present invention was excellent in the effect of inhibiting melanin formation and exhibited excellent whitening effect.

In addition, the composition according to the present invention has an effect of improving wrinkles and elasticity.

The skin wrinkles are caused by the skin of the skin and can be caused by the cause of the gene, collagen and elastin present in the skin dermis, and the external environment.

The wrinkle improvement refers to suppressing or inhibiting the generation of wrinkles, or alleviating the already generated wrinkles.

The skin elasticity refers to elastic fibers composed of elastin existing in the dermal layer and collagen fibers called collagen. The elasticity improvement refers to suppressing or inhibiting elasticity reduction or maintaining or improving elasticity .

As a result of treating the complex extract according to the present invention, the inventors of the present invention confirmed that they exhibited an enhanced collagen synthesis promoting effect and an elastase activity inhibiting effect, respectively, as compared with the case of treating each extract, And vitamin C, and the effect of quercetin, which is known to inhibit the activity of the skin elastase, is exerted. The composition according to the present invention is excellent in collagen synthesis promoting effect and elastase activity inhibiting effect and shows excellent wrinkle and elasticity improvement effect.

Further, the composition according to the present invention has an effect of improving the trouble.

The above-mentioned troubles are symptoms such as skin irritation and inflammation. The inflammatory reaction is characterized by pain, fever, redness, swelling and malfunction due to external stimuli. Histologically, the permeability of the arterioles, capillaries, , Complicated symptoms including enlargement accompanied by increase, excretion of plasma containing plasma protein, migration to inflammation site of leukocyte, and skin irritation reaction are characterized by erythema, itching, fever and the like.

The improvement of the trouble refers to inhibiting or inhibiting the skin irritation reaction and the inflammation reaction, or alleviating the skin irritation reaction or the inflammation reaction already in progress.

As a result of measuring the inhibitory effect on NO production by treating the compound extract according to the present invention, the present inventors confirmed that the inhibitory effect on NO production was enhanced compared to the case of treating each extract, and NO inhibitory effect was known And exhibited an effect corresponding to that of L-NMMA. It was confirmed that the composition according to the present invention exhibits excellent skin trouble improving effect through inhibition of NO production.

In addition, the composition according to the present invention has an antioxidative effect.

The antioxidant refers to inhibition of cellular oxidation by free radicals or reactive oxygen species (ROS), which are highly reactive according to oxidative stress caused by intracellular metabolism or ultraviolet rays, and free radicals Or removal of reactive oxygen species, thereby reducing damage to the cells.

As a result of measuring the free radical scavenging ability of the complex extract according to the present invention, the inventors of the present invention confirmed that they exhibited an increased free radical scavenging ability as compared with the case of treating each extract, and found that the free radical scavenging ability of vitamin C And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits an excellent antioxidative effect.

In addition, the composition according to the present invention has an antiherating effect.

The glycation refers to a phenomenon in which a sugar and a protein are bound to each other due to the presence of excessive sugars and the protein is destroyed. As glycosylation progresses, it affects collagen and elastin, resulting in skin elasticity deterioration and other skin damage.

As a result of measuring the antagonism effect by treating the complex extract according to the present invention, the inventors of the present invention have confirmed that they exhibit an elevated antihyperglycosylation effect as compared with the case of treating the respective extracts, and the aminoguanidine And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits excellent anticarcinogenic effect.

The complex extract according to the present invention is safe for the skin and has an excellent effect for improving the skin including anti-glycation effect, skin whitening effect, skin elasticity and wrinkle improving effect, antioxidative effect and skin trouble improving effect.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the embodiments and experimental examples according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the above-described embodiments and experiments. The embodiments and experimental examples of the present invention are provided to enable those skilled in the art to more fully understand the present invention.

< Example  1> Preparation of Sukjipu Extract

After finely drying, 3 g of dried ginger was added to a flask and 100 g of dry weight was extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a watery extract.

< Example  2> Preparation of corn oil extract

Dried corn oil was well dried, and then 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a crude oil extract.

< Example  3> Light  Preparation of extract

The dried tablets were well dried, and then placed in a flask with a dry weight of 100 g and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a crude extract.

< Example  4> Preparation of Hwanggi Extract

The dried yellow oak was thoroughly dried, and 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered through a filter having a pore size of 0.2 mu m to prepare an extract of hwanggi.

< Example  5> Preparation of raw persimmon extract

After the raw persimmon was cut three times, 100 g of the weight was placed in a flask, and the mixture was extracted by cooling with 1000 g of an extraction solvent (distilled water) for 3 days. The frozen extract was filtered with a filter having a pore size of 0.2 탆 to prepare a raw persimmon extract.

< Example  6> Preparation of extract

After drying thoroughly, 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a mulberry extract.

< Example  7> Bamboo  Preparation of extract

The scallop was dried well and cut into small pieces. 100 g of dry weight was placed in a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a bark extract.

< Example  8> Production of Gamma Extract

The hair was thoroughly dried, and 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a glutinous extract.

< Example  9> Yellowish white  Preparation of extract

The yellowish white was well dried and finely divided, and 100 g of dry weight was added to the flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a yellowish white extract.

< Example  10> Mold  Preparation of extract

The mold was well dried and cut into three pieces. 100 g of dry weight was put into a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a mold extract.

< Example  11> Sukjihwang, corn oil, Light , Hwanggi, Jijiji, Maekmundong, Bamboo However, Yellowish white  And Mold (10 kinds) Preparation of mixed extract

In the same manner as in the above-mentioned examples, the same amount of extracts of Sukjipu, Sansui, Sanjak, Hwanggi, Sejuk, Mangmundong, Seokgok, Yellow, white and moldy mixed extracts were prepared.

< Experimental Example  1> Antialcification  effect

In order to confirm anti-glycation efficacy, the present inventors measured glycosylation inhibitory activity using L-arginine and glucose.

First, 1M L-arginine and 1M glucose were dissolved by using 1M phosphate buffer (pH 7.4), and prepared by diluting the sample to 50ppm with 1M phosphate buffer solution. 1M L-arginine and 1M phosphate buffer solution were mixed at a ratio of 1: 4, and then 80 [mu] l each was added to a 96-well plate. To each sample, 100 μl of 0.01 M aminoguanidine was added to each sample to be diluted to 50 ppm and used as a positive control. These samples were mixed well and finally glucose diluted with 1M phosphate buffer solution was added to the final concentration of glucose to 0.1 M and reacted at 70 ° C for 4 hours. The degree of saccharification was measured by measuring the absorbance of the 96-well plate at 420 nm using a spectrophotometer.

Glycation group of the following formula was an experimental group in which 1M L-arginine and 1M glucose were added to induce glycation. Absorbance was measured at 420 nm by adding 1M L-arginine and sample alone without glucose to measure the absorbance of the sample itself. The saccharide inhibitory activity can be obtained by the following formula. Experiments were performed three times each and expressed as a mean value.

[Equation 1]

('Glycation test group' in the above equation (1) means 'Absorbance of Glycation test group')

sample Inhibition rate (%) The control (DMSO, 50 ppm) - The positive control (Aminoguanidine, 55 ppm) 54.89 Example 1 42.12 Example 2 45.33 Example 3 39.72 Example 4 36.91 Example 5 30.38 Example 6 41.32 Example 7 49.73 Example 8 38.74 Example 9 35.76 Example 10 44.41 Example 11 (50 ppm) 70.05

As can be seen from the results shown in Table 1, the mixed extract showed better anticarcinogenic effect than aminoguanidine, which is known as an anticarcinogenic substance.

< Experimental Example  2> Melanin formation inhibitory effect

In order to confirm the whitening effect through inhibition of melanin formation, a culture solution of B-16 mouse melanoma cells in rat was cultured according to the method described in Lotan R. et al. (Cancer Res. 40: 3345-3350, 1980) The total amount of melanin was measured by adding an extract. In the experiment, first the toxicity of melanoma cells in rats was evaluated and the whitening evaluation was carried out at a concentration that is not toxic. DMSO was used as a negative control group, and albutin was used as a positive control group.

Specifically, the sample was added to the medium to a final concentration of 100 ppm, arbutin was added to the medium to be 100 ppm, and melanoma cells were cultured for 3 days. Cells were then trypsinized, detached from the culture, centrifuged, and then melanin was extracted. The removed cells were incubated with 1 ml of sodium hydroxide solution (1N concentration), boiled for 10 minutes to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.

The amount of melanin was measured by an absorbance of 1 × 10 ⁶ cells per unit cell, and the total amount of melanin relative to the control group was calculated as the inhibition rate (%). The results are shown in Table 2 below. As shown in FIG.

sample Melanin production
(abs) Inhibition rate (%) Control group (DMSO, 10 ppm) 0.33 - Positive control (arbutin, 100 ppm) 0.228 30.91 Example 1 0.292 11.52 Example 2 0.311 5.76 Example 3 0.288 12.73 Example 4 0.259 21.52 Example 5 0.299 9.39 Example 6 0.273 17.27 Example 7 0.290 12.12 Example 8 0.301 8.79 Example 9 0.285 13.64 Example 10 0.297 10.00 Example 11 (100 ppm) 0.232 29.70

As can be seen from the results of Table 2 above, the mixed extract showed excellent melanin total amount reduction effect, which is useful for whitening purposes.

< Experimental Example  3> Anti-inflammatory effect

In order to confirm the anti-inflammatory effect and the improvement of skin trouble, nitric oxide (NO) production inhibition experiment was performed by GRIESS method using RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, macrophages of mice, were subcultured several times, placed in 24-well plates so as to enter 3x10 ⁵ wells into each well, and cultured for 24 hours. Subsequently, the cell culture medium containing the sample was replaced with a final concentration of 10 ppm. At this time, L-NMMA (L-NG-Monomethylarginine), which is an inhibitor of NO production, was treated together as a positive control and cultured for 30 minutes. Lipopolysaccharide (LPS) 100 μl of the supernatant was transferred to a 96-well plate, 100 μl of GRIESS solution was added thereto, and the reaction was allowed to proceed at room temperature for 10 minutes. The absorbance at 540 nm was measured to determine the NO inhibitory effect. Calculated using Equation (2) and shown in Table 3 below. Experiments were performed three times each and expressed as average values.

&Quot; (2) &quot;

NO production inhibition rate (%) = {(absorbance of negative control - absorbance of each extract) / absorbance of negative control} x 100

sample NO production inhibition rate (%) Control group (DMSO, 10 ppm) - L-NMMA (positive control, 10 ppm) 41.02 Example 1 39.46 Example 2 29.53 Example 3 31.26 Example 4 21.49 Example 5 39.67 Example 6 24.81 Example 7 22.61 Example 8 27.97 Example 9 34.16 Example 10 33.87 Example 11 (10 ppm) 40.10

As can be seen from the results in Table 3, the mixed extracts showed a relatively low relative activity but excellent activity as a natural substance when compared with L-NMMA, a representative anti-inflammatory drug.

< Experimental Example  4> Promoting collagen synthesis

The sample was added to the culture medium of human - derived fibroblasts to confirm the promoting effect of type I collagen synthesis at the cellular level. The synthesized collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay Kit). In order to measure the amount of collagen synthesis, the sample was added to a fibroblast culture medium (DMEM medium) at a final concentration of 10 ppm and cultured for 48 hours. The culture broth was taken and the degree of type 1 collagen synthesis was measured at each concentration using a PICP EIA kit And measured at 450 nm using a spectrophotometer.

For the comparison of the effects, the degree of collagen synthesis was measured in the same manner for the samples in which the culture medium of the untreated fibroblasts (negative control) and vitamin C (positive control) were added to a final concentration of 52.85 / / ml. The increase rate of collagen production was calculated by the ratio of relative collagen production to the negative control, and the results are shown in Table 4 below.

sample Type 1 collagen production (ng / ml) Growth rate (%) Negative control group 150.2 - Positive control (vitamin C) 246.8 64.3 Example 1 215.2 43.3 Example 2 168.3 12.1 Example 3 189.8 26.4 Example 4 176.9 17.8 Example 5 194.3 29.4 Example 6 205.6 36.9 Example 7 188.0 25.2 Example 8 213.7 42.3 Example 9 200.9 33.8 Example 10 221.1 47.2 Example 11
(10 ppm) 246.3 64.0

As can be seen from the results in Table 4, the collagen synthesis was increased when the mixed extract was treated, and the collagen synthesis effect was similar to that of vitamin C, which is generally known to induce collagen synthesis.

< Experimental Example  5> Ella Stays  Active inhibitory effect

The activity inhibitory effect of Elastase, an enzyme that degrades elastin, was confirmed as follows.

Elastase used Elastase from human leukocyte cells and MeOSuc-Ala-Ala-Pro-Val-pNA as synthetic substrate for Elastase. The buffer solution used was 100 mM Tris (pH 7.5) solution. Finally, 0.2 mU was used for the ELASTASES using a buffer solution. In addition, the synthetic substrate of Elastinase was diluted with a buffer solution to make a final concentration of 0.5 mM after making a 100 mM solution using DMSO. At this time, the positive control group was set to contain 10 ppm of quercetin, which is known as an inhibitor of Elastinase. Ellazease inhibition candidates were added to give a final concentration of 10 ppm. The reaction was carried out in a 96-well plate and allowed to react at room temperature for 20 minutes. Absorbance was measured at 405 nm using a spectrophotometer at intervals of 1 minute, and the slope of the absorbance versus time was determined as the activity of the enzyme. Ella stasis inhibition rates were calculated as follows.

&Quot; (3) &quot;

The inhibition rate of the ELA stase was calculated using the above equation (3) and shown in the following Table 5, and the experiment was performed three times each and expressed as an average value.

sample Enzyme activity Inhibition rate (%) Example 1 7.9 24.04 Example 2 6.2 40.38 Example 3 4.3 58.65 Example 4 8.1 22.12 Example 5 5.2 50.00 Example 6 5.7 45.19 Example 7 6.5 37.50 Example 8 4.7 54.80 Example 9 4.2 59.62 Example 10 6.5 37.50 Example 11
(10 ppm) 3.8 63.46 The positive control (Quercetin, 10 ppm) 3.5 66.35 The control (DMSO, 20 ppm) 10.4 -

As can be seen from the results of Table 5, when the mixed extract was treated, it was found that Elastase activity inhibitory effect excelling even in comparison with the positive control group, quercetin, was exerted. Therefore, it was found that the mixed extract can be used for skin regeneration and wrinkle improvement.

< Experimental Example  6> Antioxidant effect

The free radical scavenging ability of the mixed extract was measured by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) method (Blois, Nature 181, 1190, 1958). DPPH is a relatively stable free radical, which shows maximum absorption at 517 nm in the presence of radicals and loses its absorbance when radicals are eliminated. DPPH was purchased from Sigma, and dissolved in methyl alcohol at a concentration of 0.15 mM.

First, 100 μl of mixed extract or vitamin C, a positive control, was added to each well of a 96-well plate. 100 μl of DPPH solution was added thereto, and the mixture was allowed to stand at room temperature for 30 minutes, and the absorbance at 517 nm was measured using a microplate reader (BioTek EL-340).

IC ₅₀ represents the concentration of the extract when the absorbance of the sample is half the absorbance of the control group. The results are shown in Table 6 below. This experiment was repeated 3 times.

sample IC50 (% w / v) Control group 0.0025 Positive control (vitamin C) concentration 0.0005 Example 1 Concentration 0.0020 Example 2 Concentration 0.0012 Example 3 Concentration 0.0018 Example 4 Concentration 0.0024 Example 5 Concentration 0.0017 Example 6 Concentration 0.0019 Example 7 Concentration 0.0010 Example 8 Concentration 0.0023 Example 9 Concentration 0.0011 Example 10 Concentration 0.0015 Example 11 Concentration 0.0007

As shown in Table 6, the mixed extracts showed a strong free radical scavenging ability as compared with vitamin C, confirming that the antioxidative effect was excellent.

Claims (4)

Skin whitening, wrinkle improvement, elasticity improvement, trouble-improving or antioxidant cosmetic composition containing Sukjipu extract, Sansori oil extract, Acanthopanax extract, Huangjiekisu extract, Seiji extract, Mukmun dong extract, Gigok extract, Gamma extract, Composition. The method according to claim 1, wherein the weight ratio of the extract is from 1: 0.1 to 10: 0.1 to 10: 0.1 to 0.1: 1, and the weight ratio of the extract to the extract is from 1: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10. 3. The cosmetic composition according to claim 1 or 2, wherein the extract is extracted with a solvent selected from the group consisting of water, C1 to C4 lower alcohols or a mixture thereof. The cosmetic composition according to claim 1, wherein the cosmetic composition is antialcification, inhibition of melanin formation, promotion of collagen synthesis, inhibition of elastase activity or free radical scavenging.