KR20170137552A

KR20170137552A - Composition for improving skin condition comprising herb extracts mixture

Info

Publication number: KR20170137552A
Application number: KR1020160069858A
Authority: KR
Inventors: 김병현; 진무현; 황승진; 노효선; 노석선; 최인화; 이명수
Original assignee: 주식회사 엘지생활건강
Priority date: 2016-06-03
Filing date: 2016-06-03
Publication date: 2017-12-13

Abstract

The present invention relates to a composition containing as an active ingredient a complex herbal extract which is safe when applied to skin and has excellent skin improving effect. More specifically, the composition according to the present invention can be used as a composition for preventing or treating cancer, such as angelica gigantis extract, gigyeong extract, fenugreek extract, hinoki extract, fenugreek extract, mungumundong extract, Skin composition, skin whitening effect, skin elasticity and wrinkle improvement effect, antioxidative effect and skin trouble improvement effect by the composition comprising the extract of Hwanggi, Jibusa extract, .

Description

[0001] The present invention relates to a composition for improving skin comprising a herbal extract,

The present invention relates to a composition containing as an active ingredient a complex herbal extract which is safe when applied to skin and has excellent skin improving effect.

The skin is an important body that is responsible for various physiological functions such as barrier function, body temperature control function, excretion function, which protects the human body from the external environment and prevents the internal moisture and useful components from flowing out. However, due to the following reasons, the activity of skin cells may deteriorate and the skin condition may deteriorate.

Collagen and elastin present in the dermis of the skin perform functions such as mechanical rigidity of the skin, resistance of the connective tissue, maintenance of the cohesion of the tissue, and support of the cell adhesion, and have a great influence on the constituents of the skin. It is known that such collagen is reduced by photoaging due to stress, aging, or ultraviolet irradiation, while elastin is known to be distorted in its three-dimensional structure by the degrading enzyme elastase, which is activated after exposure to ultraviolet light. As a result, the skin tissue is loosened and the elasticity is lost.

When the cell function of the epidermis is lowered, the metabolism is not smooth and the exfoliation does not occur well, and when the ultraviolet rays are received in a state where the keratin is overly accumulated, the elasticity is lowered and wrinkles are generated. If the skin is not formed on the epidermis, moisture and sebum secretion are reduced, and the skin becomes dry and wrinkles are formed.

When the skin receives ultraviolet rays, melanin is synthesized to protect the skin. The synthesized melanin is transferred to the keratinocytes of the skin through the melanoma. This keratinocyte turns over over a period of 28 days. Therefore, the melanin produced is generally lost by the keratinocyte periodically for 28 days. However, when the skin cell cycle is not properly controlled by the stress, skin aging, etc., keratinocytes do not fall out in the presentation period and the pigment such as spots, freckles, Calm occurs.

In the skin, sebum, sweat and cosmetic ingredients are decomposed into substances that are toxic to the skin by the fungus, which can cause skin irritation and inflammation. In addition, skin irritation caused by ultraviolet rays increases the production of nitrogen monoxide (NO), which is an inflammation mediator, to cause skin troubles. The production of nitrogen monoxide is mostly caused by iNOS, and it is known that iNOS is rapidly induced by stimulation such as LPS and cytokine to produce excessive NO.

A reactive oxygen species (ROS), also known as a toxic oxygen species, is a cell-generated toxic substance produced by physiological actions such as respiration and is constantly produced and extinguished, with 3-5% in normal conditions . These reactive oxygen species are free radicals such as superoxide radicals (O2-) and hydroxyl radicals (HO +), which are chemically bound to atoms or molecules that are not paired with the outermost electron orbits Highly unstable and highly reactive) or in the form of compounds with paired electrons such as hydrogen peroxide (H ₂ O ₂ ) or singlet radicals. Active oxygen species have the advantage of biologically protecting bacteria in the physiological system, but generally cause oxidation in vivo, causing harmful effects that cause disease. It has been reported that these reactive oxygen species attack biological molecules and damage cells and tissues and cause various diseases related to aging and various diseases.

Research has been continued to suppress the skin aging caused by various factors as described above, to improve whitening, wrinkles, elasticity or trouble, and to develop a substance having an antioxidative effect.

Materials having the above-mentioned effects are widely distributed in the natural world, and they have been mainly used as raw materials for foods, cosmetics, medicines and the like using materials derived from plants. However, the substances derived from such natural substances are not effective enough to be used in large quantities in order to obtain a meaningful effect, resulting in toxicity and price increase.

In order to solve the problems of such natural materials, the development of chemically synthesized materials has continued. Although they have a merit that they exert a superior effect even when used in a small amount compared to a natural substance, their use is limited due to a fatal problem that can cause large and small side effects to the human body.

Accordingly, it is inevitable to develop a substance derived from a natural product and having stability, while exhibiting an excellent effect on skin improvement.

KR 1020060092179 A

A problem to be solved by the present invention is to provide a complex herbal medicine extract which is derived from natural materials and has stability, while exhibiting excellent effects on skin, in particular whitening, wrinkle, elasticity, trouble improvement and antioxidation.

In order to solve the above-mentioned problems, the present invention provides a method for the production of a medicament for the treatment of angiogenesis, (For example, a cosmetic composition) for skin whitening, wrinkle improvement, elasticity improvement, trouble improvement, or antioxidation, wherein the composition contains, as an active ingredient, an extract of Hwanggi, a mushroom extract, a mulberry extract, a mulberry extract,

[extract]

Angelica Gigas belongs to the persimmon, and contains the roots of Angelica Gigas Nakai . It has the property to make the body and the body healthy. The body of Angelica Gigas regulates the blood and the upper part of the Angelica gigas Has the effect of making blood.

Gyungyeong (桔梗) Platycodon bellflower grandiflorum A. De Candolle (Campanulaceae), or as root.

Peony (迦药) Peony Paeonia It is the root of lactiflora Pallas or other related plants (Paeoniaceae and Paeoniaceae).

Rehmannia (地黄) is the Rehmannia glutinosa (Gaertner) Liboschitz ex Steudel (ginseng and Scrophulariaceae) fresh roots.

The moldings are made of Schizonepeta tenuifolia Briquet (Lamiaceae and Labiatae) is a flowering Isaac.

Macmun-dong is a perennial herbaceous plant belonging to the Liliaceae, Liriope platyphylla Wang et Tang or Ophiopogon japonicus Ker-Gawler) is the bulge of the root.

White tine bark (白鮮皮) is a white line Dictamnus It is the root shell of dasycarpus Turczaininov (rhubarb and Rutaceae).

The casualties (蛇床子) are the casualties Cnidium monieri (L). It is the fruit of Cussion or casualties Torilis japonica Decandolle (hermaphrodite and Umbelliferae).

Licorice (licorice, Glycyrrhizae Radix) is a perennial herb belonging to leguminosae, Glycyrrhiza uralensis Fischer, licorice and licorice Glycyrrhiza glabra Linne or licorice and licorice Glycyrrhiza inflata Batal's roots and rootstocks are as they are or have been removed.

Windbreaks are the roots of the windshield Saposhnikovia divaricata Schischkin (mountainous and Umbelliferae).

Grass (紫草) is a ditch Lithospermum erythrorhizon Siebold et Zuccarini, Xinjiang Zhejiang Arnebia euchroma Johnst. Naemong or self-inflicted (內蒙紫草) is the root of Arnebia guttata Bunge (not dental Boraginaceae)

The ginseng is the Latin name Scrophulariae Radix, Scrophularia buergeriana Miquel or Chinese ginseng Scrophularia ningpoensis refers to the roots of Hemsley ( hyacinth and Scrophulariaceae). There is a peculiar smell like sugar burning, a little taste and a little bit after. It is reported that it can be used as a fever reducing agent for sore throat, boils, and lymphatic arthritis. It grows in the mountains.

Gosam (苦) is the Gosam Sophora flavescens Solander ex Aiton (leguminosae) as a root or as is removed.

Hwanggi belongs to Leguminosae (Fabaceae), and it is the root of the scientific name Astragalus membranaceus Bunge either as it is or has been removed. It is a thin, long, circumferential, 30-100 cm long, 7-20 mm in diameter. It has sparse roots but does not branch off. It is slightly twisted near the head toes and remains stalks. Outer surface is pale yellowish yellow to pale yellowish brown, grayish brown cork layer sometimes remains, and irregular rough vertical wrinkles and horizontal like pimples are seen. The quality is dense and hard to break, and the folded side is fibrous.

Kochiae Fructus is a fruit of Kochia scoparia Schrader belonging to Chenopodiaceae.

It is the roots of Paeonia suffruticosa Andrews (peony and Paeoniaceae). It is tubular-semicircular peel, 5-8 cm long, 10-15 mm in diameter, 2-6 mm thick. Outer surface is dark brown-purple brown with horizontal long side and small elliptical side root marks and vertical wrinkles. On the inside side is light gray brown-dark purple colored, flattened surface is rough. White crystals are sometimes attached to the inner side and the folded side. It is well-known that the five-pills are comfortable and the blood vessels flow well.

Bokryeong (茯) is the sclerot of the scientific name Wolfiporia extensa (Porcine mushroom and Polyporaceae). Sclerotia is lumpy and usually consists of crushed or cut pieces. The whole is 10 to 30 cm in diameter and 0.1 to 2 kg in weight. The remaining crust is dark brown to dark reddish brown with rough and open gaps. The genus is white or light reddish white, the vagina is hard and easy to break. It is almost odorless and taste is mild and slightly mucous.

Takasago (澤泻) is a tuber of Alisma orientale Juzepzuk (Tag apple Alismataceae), which is a plant of Phytoplankton. It is said to be a deciduous herb. Root stems are short, forming roots, and have many beard roots. From the top of the stem, three or four branches are turned. It is used for ornamental and medicinal purposes. The rootstock is called a taxa and is used as a medicinal product.

The present inventors have found that all of the above extracts have biological activities beneficial to the skin, and that the extracts of two or more of the above extracts, preferably Angelica gigantosa extract, Gakyoung extract, Peony root extract, Rhizopus extract, Fusarium root extract, , A licorice extract, a windbreak extract, a herb extract, an acorn extract, a ginseng extract, a hwanggi extract, a zygote extract, a herbal extract, a ginseng extract and a taxa extract have synergistic biological activities.

In the present invention, the term "extract" is intended to mean an extract obtained by the above-mentioned extraction treatment, a diluted or concentrated liquid of the extracted liquid, a dried material obtained by drying the extracted liquid, a controlled preparation or a purified product of the extracted liquid, And extracts of all formulations which can be formed using extracts.

Each of the above-mentioned extracts describes each plant and may include leaves, stems, bark, roots, flowers or flowers, fruit, seeds, sap, and whole plants in addition to the specified parts.

In order to prepare the above extract, one of ordinary skill in the art can use any suitable method known in the art. For example, a solvent extraction method can be used. The entire plant or any portion thereof may be ground (e.g., a blender) and then the extraction solvent may be treated to obtain a solvent extract. It may be pulverized after the drying process (for example, drying at 40 to 70 for 15 to 50 hours) before pulverization. In addition, the solvent extract may be prepared in powder form by an additional process such as vacuum distillation and freeze-drying or spray-drying.

The kind of the extraction solvent used is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or mixtures thereof. Water and lower alcohols may be used alone or in combination of two or more. The solvent extract may be prepared by extracting the extract at least one time using the solvent, and the dry extract obtained by vacuum distillation or spray drying the solvent extract may be prepared.

The amount of the extraction solvent may vary depending on the kind of the extraction solvent used, but may be, for example, 1 to 20 times, or 5 to 20 times the dry weight of the target plant.

In addition, various extraction processes known in the art such as, for example, maceration, infusion, percolation, digestion, decoction, hot continuous extraction, aqueous- (For example, hydrofluoro-carbon solvent), etc.), which may be used alone or in combination with one another, may be used, for example, May be carried out by using two or more methods in combination.

[Composition]

The composition according to the present invention can be used as an antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, antioxidant, Extract, mulberry extract, bamboo extract, and phytase extract as active ingredients.

In the present invention, the term "included as an active ingredient" means that the extract is added to the skin composition of the present invention to such an extent that it can exhibit a skin improving effect, and various components are added as a sub ingredient And it is possible to formulate in various forms.

Each plant extract may be included in the composition according to the present invention in the following proportions. For example, Ginseng Extract: Gwangyang Extract: Peony Extract: Chrysanthemum Extract: Chrysanthemum Extract: Chrysanthemum Extract: Chrysanthemum Extract: Chrysanthemum Extract: Chrysanthemum Extract: 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 1, 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10, 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10: 0.1 to 10 or 1: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10: 1 to 10, or 1: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 5: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 5: 1 to 5: 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3: 1 to 3.

The compositions according to the present invention may contain at least about 0.0001%, 0.0002%, 0.0003%, 0.0004%, 0.0005%, 0.0006%, 0.0007%, 0.0008%, 0.0009%, 0.0010%, 0.0011%, 0.0012% , 0.0014%, 0.0015%, 0.0016%, 0.0017%, 0.0018%, 0.0019%, 0.0020%, 0.0021%, 0.0022%, 0.0023%, 0.0024%, 0.0025%, 0.0026% %, 0.0031%, 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0037%, 0.0038%, 0.0039%, 0.0040%, 0.0041%, 0.0042% 0.005%, 0.0057%, 0.0058%, 0.0059%, 0.0060%, 0.0061%, 0.0062%, 0.0063%, 0.0058% , 0.0064%, 0.0065%, 0.0066%, 0.0067%, 0.0068%, 0.0069%, 0.0070%, 0.0071%, 0.0072%, 0.0073%, 0.0074%, 0.0075%, 0.0076%, 0.0077% %, 0.0081%, 0.0082%, 0.0083%, 0.0084%, 0.0085%, 0.0086%, 0.0087%, 0.0088%, 0.0089%, 0.0090%, 0.0091%, 0.0092%, 0.0093%, 0.0094%, 0.0095% 0.0097%, 0.0098%, 0.0099%, 0.0100%, 0.0200%, 0.0250%, 0.0275 %, 0.0300%, 0.0325%, 0.0350%, 0.0375%, 0.0400%, 0.0425%, 0.0450%, 0.0475%, 0.0500%, 0.0525%, 0.0550%, 0.0575%, 0.0600%, 0.0625%, 0.0650% 0.0725%, 0.0725%, 0.0750%, 0.0775%, 0.0800%, 0.0825%, 0.0850%, 0.0875%, 0.0900%, 0.0925%, 0.0950%, 0.0975%, 0.1000%, 0.1250%, 0.1500%, 0.1750%, 0.2000% , 0.2250, 0.2500, 0.2750, 0.3000, 0.3250, 0.3500, 0.3750, 0.4000, 0.4250, 0.4500, 0.4750, 0.5000, 0.5250, 0.550, 0.5750, 0.6000, 0.6250 %, 0.6500%, 0.6750%, 0.7000%, 0.7250%, 0.7500%, 0.7750%, 0.8000%, 0.8250%, 0.8500%, 0.8750%, 0.9000%, 0.9250%, 0.9500%, 0.9750%, 1.0% 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9% , 2.9%, 3.0%, 3.1%, 3.2%, 3.3%, 3.4%, 3.5%, 3.6%, 3.7%, 3.8%, 3.9%, 4.0%, 4.1%, 4.2% 5.4%, 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 4.6%, 4.7%, 4.8%, 4.9%, 5.0%, 5.1%, 5.2% 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7.0%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7% , 7.9%, 8. 9.2%, 9.4%, 9.4%, 9.5%, 9.6%, 8.3%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 9.0% , 9.7%, 9.8%, 9.9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21% %, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 35%, 40%, 45%, 50%, 60%, 65%, 70%, 75% 85%, 90%, 95%, or 99% or more of the extract of the present invention. The% can be calculated on the basis of the total weight of the composition or the volume relative to the total volume, and the concentration can be adjusted according to the desired effect of the composition or the product into which the composition is incorporated.

The compositions of the present invention may be formulated into any type of vehicle. Examples of suitable vehicles include, but are not limited to, emulsions (e.g., oil, water, water in silicone, water in silicone, water in heavy oil, water in oil, water in heavy water, But are not limited to, a hydro-alcoholic solution), a dry basis (e.g., lipstick and powder), a gel, ointment, paste, milk, liquid, aerosol, solid form or eye jelly.

The compositions of the present invention may also be encapsulated for delivery to a target area, such as the skin. Encapsulation techniques include, for example, the use of liposomes, follicles, and / or nanoparticles (e. G., Components that are trapped, encapsulated, and / or absorbed polymeric materials that can be used as a delivery vehicle But are not limited to, the use of biodegradable and non-biodegradable colloidal particles, including, for example, nanospheres and nanocapsules.

The composition according to the present invention can be variously commercialized. For example, cosmetic compositions, food compositions, and the like.

The cosmetic composition may be prepared, for example, in the form of a general emulsified formulation and a solubilized formulation. For example, creams, essences, serums, cosmetic ointments, sprays, oil gels, gels such as lotions such as lotion, facial lotion, body lotion and the like such as flexible lotion or nutrition lotion, nutrition cream, A lotion, a cleansing lotion, a makeup remover such as a cleansing oil, a cleansing foam, a soap, a body wash and the like such as a foundation, a pack, a sunscreen, a makeup base, a liquid type, a solid type or a spray type, But may be formulated into various forms known in the art without limitation.

In addition to the extract according to the present invention, the above-mentioned cosmetic composition may further include optional ingredients commonly known as ingredients of the cosmetic composition in the art, so long as the object of the present invention is not impaired. Emulsifying agents, stabilizers, lubricants, solvents, moisturizers (e.g. emollients, humectants, film forming agents, occlusive agents, emulsifiers, water-repellant, a UV absorber (including physical and chemical absorbents such as para-aminobenzoic acid ("PABA") and corresponding (Eg, PABA derivatives, titanium dioxide, zinc oxide, etc.), essential oils, vitamins such as A, B, C, D, E and K, trace metals such as zinc, calcium and selenium, Antioxidants (such as BHT and tocopherol), chelating agents (such as disodium EDTA and tetrasodium EDTA), antioxidants (for example, antimicrobial agents) , Preservatives (such as methylparaben and propylparaben), pH adjusting agents (such as sodium hydroxide and citric acid) (E.g., aluminum starch octenyl succinate, kaolin, corn starch, oat starch, cyclodextrin, talc, and zeolites), skin bleaching and lightening agents (e.g., Hydroquinone and beta-hydroxynaphthoic acid (e.g., hydroquinone and niacinamide lactate), wetting agents such as glycerin, propylene glycol, butylene glycol, pentylene glycol, sorbitol, urea and mannitol, exfoliants (E.g., magnesium / aluminum hydroxide stearate), skin conditioning agents (e.g., aloe extracts), and the like, , Allantoin, bisabolol, ceramide, dimethicone, hyaluronic acid, and dipotassium glycyrrhizate), and thickening agents (e.g., (E.g., silicone oils and polyorganosiloxanes), and the like, which are capable of increasing viscosity, such as carboxylic acid polymers, crosslinked polyacrylate polymers, polyacrylamide polymers, polysaccharides and gums, .

The food composition may be formulated into, for example, beverages, fortified water, energy drinks, nutritional drinks, solid foods, vitamins, supplements, etc., But is not limited thereto. Such adjuvants may include vitamins, minerals, herbs or other plants, amino acids, enzymes and metabolites. Such adjuvants are suitable for oral consumption and may be administered orally.

May be provided as a kit comprising the composition according to the present invention. The composition according to the invention is contained in a container which can contain a bottle, a metal tube, a laminate tube, a plastic tube, a dispenser, a pressure vessel, a barrier container, a package, a compartment, a lipstick container, a compact container, A cosmetic pan, or other type of container, including, but not limited to, a plastic container that is injected or blow-molded into a suitable bottle, dispenser, or package to be maintained or dispersed, . The kit may include an instruction for using the kit or composition, the instruction sheet may be described on a separate sheet, or may be described on the surface of the container surface, the surface of the container wrapper. Instructions include, but are not limited to, letters, phrases, abbreviations, pictures or symbols. The instructions may include, for example, instructions on how to use, apply and maintain the kit or composition. The container can be dispensed according to a predetermined amount.

The composition according to the present invention may be provided as a topical skin composition.

In addition, a method of topically applying the composition according to the present invention to the skin can be provided.

In the present invention, the term "topical application" means applying or applying the composition on the surface of keratinous tissue, and "topical skin composition" includes compositions suitable for topical application or application on keratinocytes. Such compositions are typically dermatologically acceptable in that they do not have excessive toxicity, incompatibility, instability, allergic response, etc. when applied to the skin. The topical skin care compositions of the present invention may have a selected viscosity to avoid significant dripping or pooling after application to the skin.

[Skin improvement effect]

The composition according to the present invention has biological activity and exerts excellent effects on skin improvement.

More specifically, the composition according to the present invention has a skin whitening effect.

The skin whitening refers to inhibiting melanin formation and inhibiting melanin formation.

As a result of measuring the amount of melanin produced by treating the melanoma cells according to the present invention, the inventors of the present invention confirmed that the melanin production inhibitory effect was enhanced compared with the case of treating each extract, It was found that the effect exerted an effect corresponding to known arbutin. It was confirmed that the composition according to the present invention was excellent in the effect of inhibiting melanin formation and exhibited excellent whitening effect.

In addition, the composition according to the present invention has an effect of improving wrinkles and elasticity.

The skin wrinkles are caused by the skin of the skin and can be caused by the cause of the gene, collagen and elastin present in the skin dermis, and the external environment.

The wrinkle improvement refers to suppressing or inhibiting the generation of wrinkles, or alleviating the already generated wrinkles.

The skin elasticity refers to elastic fibers composed of elastin existing in the dermal layer and collagen fibers called collagen. The elasticity improvement refers to suppressing or inhibiting elasticity reduction or maintaining or improving elasticity .

As a result of treating the complex extract according to the present invention, the present inventors confirmed that they exhibited an enhanced collagen synthesis promoting effect and an elastase activity inhibiting effect, respectively, as compared with the case of treating each extract, And the effect of inhibiting the activity of skin elastase was shown to exert an effect corresponding to the known quercetin. The composition according to the present invention is excellent in collagen synthesis promoting effect and elastase activity inhibiting effect and shows excellent wrinkles and elasticity improving effect.

Further, the composition according to the present invention has an effect of improving the trouble.

The above-mentioned troubles are symptoms such as skin irritation and inflammation. The inflammatory reaction is characterized by pain, fever, redness, swelling and malfunction due to external stimuli. Histologically, the permeability of the arterioles, capillaries, , Complicated symptoms including enlargement accompanied by increase, excretion of plasma containing plasma protein, migration to inflammation site of leukocyte, and skin irritation reaction are characterized by erythema, itching, fever and the like.

The improvement of the trouble refers to inhibiting or inhibiting the skin irritation reaction and the inflammation reaction, or alleviating the skin irritation reaction or the inflammation reaction already in progress.

As a result of measuring the inhibitory effect on NO production by treating the compound extract according to the present invention, the present inventors confirmed that the inhibitory effect on NO production was enhanced compared to the case of treating each extract, and NO inhibitory effect was known And exhibited an effect corresponding to that of L-NMMA. It was confirmed that the composition according to the present invention exhibits excellent skin trouble improving effect through inhibition of NO production.

In addition, the composition according to the present invention has an antioxidative effect.

The antioxidant refers to inhibition of cellular oxidation by free radicals or reactive oxygen species (ROS), which are highly reactive according to oxidative stress caused by intracellular metabolism or ultraviolet rays, and free radicals Or removal of reactive oxygen species, thereby reducing damage to the cells.

As a result of measuring the free radical scavenging ability of the complex extract according to the present invention, the inventors of the present invention confirmed that they exhibited an increased free radical scavenging ability as compared with the case of treating each extract, and found that the free radical scavenging ability of vitamin C And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits an excellent antioxidative effect.

In addition, the composition according to the present invention has an antiherating effect.

The glycation refers to a phenomenon in which a sugar and a protein are bound to each other due to the presence of excessive sugars and the protein is destroyed. As glycosylation progresses, it affects collagen and elastin, resulting in skin elasticity deterioration and other skin damage.

As a result of measuring the antagonism effect by treating the complex extract according to the present invention, the inventors of the present invention have confirmed that they exhibit an elevated antihyperglycosylation effect as compared with the case of treating the respective extracts, and the aminoguanidine And the effect is comparable to that of the conventional method. Thus, it was confirmed that the composition according to the present invention exhibits excellent anticarcinogenic effect.

The complex extract according to the present invention is safe for the skin and has an excellent effect for improving the skin including anti-glycation effect, skin whitening effect, skin elasticity and wrinkle improving effect, antioxidative effect and skin trouble improving effect.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the embodiments and experimental examples according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the above-described embodiments and experiments. The embodiments and experimental examples of the present invention are provided to enable those skilled in the art to more fully understand the present invention.

&Lt; Example 1 >

The Angelica gigas was dried well and cut into three pieces. 100 g of dry weight was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered through a filter having a pore size of 0.2 탆 to prepare Angelica giganta Radix extract.

Example 2: Preparation of Ganoderma lucidum extract

Gakugei was well dried and cut into three pieces. 100 g of dry weight was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a Gwangyang extract.

Example 3: Preparation of peony root extract

After drying the pellets well, the pellets were placed in a flask with a dry weight of 100 g, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a peony root extract.

<Example 4> Preparation of a ginger extract

After the slices were well dried, the slices were placed in a flask with a dry weight of 100 g and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a rhizome extract.

Example 5: Preparation of mold extracts

The mold was well dried and cut into three pieces. 100 g of dry weight was put into a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a mold extract.

&Lt; Example 6 > Preparation of Macromolecular Extracts

After drying thoroughly, 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a mulberry extract.

Example 7: Preparation of white roots extract

The white blood was well-dried, and after three-fold drying, 100 g of dry weight was added to the flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a white line extract.

Example 8: Preparation of caspase extract

After the casualties were well dried and cut into three pieces, 100g of dry weight was put into a flask and extracted with 1000g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a caspase extract.

Example 9 Preparation of licorice extract

The licorice was dried well, and the dried weight of 100 g was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a licorice extract.

Example 10: Preparation of windshield extract

After drying thoroughly with a windshield, the dried weight of 100 g was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a windshield extract.

Example 11: Preparation of a herb extract

The pellet was dried well, and then 100 g of the dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a herb extract.

<Example 12> Preparation of ginseng extract

The dried ginseng was thoroughly dried and finely divided. The dried weight of 100 g was placed in a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered through a filter having a pore size of 0.2 탆 to prepare an extract of ginseng.

&Lt; Example 13 > Preparation of Gosam Extract

The ginseng was thoroughly dried and finely divided. The dried weight of 100 g was placed in a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a ginseng extract.

Example 14: Preparation of Hwanggi extract

The dried yellow oak was thoroughly dried, and 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered through a filter having a pore size of 0.2 mu m to prepare an extract of hwanggi.

&Lt; Example 15 > Production of extract of leaf extract

The papers were dried and cut three times. 100 g of dry weight was placed in a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a leaf extract.

<Example 16> Preparation of extract of Mulberry

After thoroughly drying the slices, the dried weight of 100 g was placed in a flask, and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a herringbone extract.

<Example 17> Preparation of extract

After thoroughly drying the bamboo shoots, the dried weight of 100 g was put into a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 탆 to prepare a bamboo shoot extract.

Example 18 Preparation of Taxa extract

The dried tablets were thoroughly dried, and then 100 g of dry weight was placed in a flask and extracted with 1000 g of an extraction solvent (distilled water) for 3 days by cooling. The frozen extract was filtered with a filter having a pore size of 0.2 mu m to prepare a taxa extract.

<Example 19> Preparation of mixed extracts of Angelica gigas Nakai, Gyungyang, Peony root, Rhizopus, Chrysanthemum, Maekmoongdong, White roe, Casualties, Licorice, Windblown,

In the same manner as in the above-mentioned examples, the same amounts of the extracts were injected in the form of extracts such as Angelica gigas, Gyungyang, Gyungsang, Gyuwang, Kyeonggi, Maekmundong, White pine bark, Casualties, Licorice, Windblown, Mixed extracts were prepared.

&Lt; Experimental Example 1 >

In order to confirm anti-glycation efficacy, L-arginine and glucose were used to measure glycosylation-inhibiting activity.

First, 1M L-arginine and 1M glucose were dissolved by using 1M phosphate buffer (pH 7.4), and prepared by diluting the sample to 50ppm with 1M phosphate buffer solution. 1M L-arginine and 1M phosphate buffer solution were mixed at a ratio of 1: 4, and then 80 [mu] l of each was added to a 96-well plate. To each sample, 100 μl of 0.01 M aminoguanidine to be used as a positive control and a sample diluted to 50 ppm were added. These samples were mixed well, and finally glucose was added to 1 M phosphate buffer solution so that the final concentration of glucose was 0.1 M, followed by reaction at 70 for 4 hours. The degree of saccharification was measured by measuring the absorbance of the 96-well plate at 420 nm using a spectrophotometer.

Glycation group of the following formula was an experimental group in which 1M L-arginine and 1M glucose were added to induce glycation. Absorbance was measured at 420 nm by adding 1M L-arginine and sample alone without glucose to measure the absorbance of the sample itself. The saccharide inhibitory activity can be obtained by the following formula. Experiments were performed three times each and expressed as a mean value.

[Equation 1]

('Glycation test group' in the above equation (1) means 'Absorbance of Glycation test group').

sample Inhibition rate (%) The control (DMSO, 50 ppm) - The positive control (Aminoguanidine, 55 ppm) 54.89 Example 1 21.99 Example 2 30.61 Example 3 31.50 Example 4 25.72 Example 5 28.95 Example 6 28.17 Example 7 35.82 Example 8 36.11 Example 9 38.15 Example 10 28.61 Example 11 29.56 Example 12 23.47 Example 13 32.73 Example 14 34.43 Example 15 39.09 Example 16 43.98 Example 17 34.77 Example 18 33.23 Example 19
(50 ppm) 55.77

As shown in the results of Table 1 above, it can be seen that the mixed extract is superior to the aminoguanidine, which is known as an anticarious substance, in the anticarcinogenic effect.

<Experimental Example 2> Effect of inhibiting melanin formation

In order to confirm the whitening effect through inhibition of melanin formation, a culture solution of B-16 mouse melanoma cells in rat was cultured according to the method described in Lotan R. et al. (Cancer Res. 40: 3345-3350, 1980) The total amount of melanin was measured by adding an extract. In the experiment, first the toxicity of melanoma cells in rats was evaluated and the whitening evaluation was carried out at a concentration that is not toxic. DMSO was used as a negative control group, and albutin was used as a positive control group.

Specifically, the sample was added to the medium to a final concentration of 100 ppm, arbutin was added to the medium to be 100 ppm, and melanoma cells were cultured for 3 days. Cells were then trypsinized, detached from the culture, centrifuged, and then melanin was extracted. The removed cells were incubated with 1 ml of sodium hydroxide solution (1N concentration), boiled for 10 minutes to dissolve melanin, and the absorbance was measured at 400 nm using a spectrophotometer to measure the amount of melanin produced.

The amount of melanin was measured by an absorbance of 1 × 10 ⁶ cells per unit cell, and the total amount of melanin relative to the control group was calculated as inhibition rate (%). The results are shown in Table 2 below. And the average value.

sample Melanin production
(abs) Inhibition rate (%) Control group (DMSO, 10 ppm) 0.33 - Positive control (arbutin, 100 ppm) 0.228 30.91 Example 1 0.301 8.79 Example 2 0.298 9.70 Example 3 0.272 17.58 Example 4 0.299 9.39 Example 5 0.289 12.42 Example 6 0.276 16.36 Example 7 0.301 8.79 Example 8 0.302 8.48 Example 9 0.299 9.39 Example 10 0.291 11.82 Example 11 0.288 12.73 Example 12 0.282 14.55 Example 13 0.311 5.76 Example 14 0.309 6.36 Example 15 0.293 11.21 Example 16 0.285 13.64 Example 17 0.271 17.88 Example 18 0.295 10.61 Example 19 (100 ppm) 0.234 29.09

As can be seen from the results of Table 2 above, the mixed extract showed excellent melanin total amount reduction effect and was found to be useful for whitening purposes.

<Experimental Example 3> Anti-inflammatory effect

In order to confirm the anti-inflammatory effect and the improvement of skin trouble, nitric oxide (NO) production inhibition experiment was performed by GRIESS method using RAW264.7 cell line (ATCC number: CRL-2278).

Specifically, RAW264.7 cells, macrophages of mice, were subcultured several times, placed in 24-well plates so as to enter 3x10 ⁵ wells into one well, and cultured for 24 hours. Subsequently, the cell culture medium containing the sample was replaced with a final concentration of 10 ppm. At this time, L-NMMA (L-NG-Monomethylarginine), which is an inhibitor of NO production, was treated together as a positive control and cultured for 30 minutes. Lipopolysaccharide (LPS) 100 μl of the supernatant was transferred to a 96-well plate, 100 μl of GRIESS solution was added thereto, and the reaction was allowed to proceed at room temperature for 10 minutes. The absorbance at 540 nm was measured to determine the NO inhibitory effect. Calculated using Equation (2) and shown in Table 3 below. Experiments were performed three times each and expressed as average values.

&Quot; (2) "

NO production inhibition rate (%) = {(absorbance of negative control - absorbance of each extract) / absorbance of negative control} x 100

sample NO production inhibition rate (%) Control group (DMSO, 10 ppm) - L-NMMA (positive control, 10 ppm) 41.02 Example 1 35.22 Example 2 35.19 Example 3 35.31 Example 4 29.49 Example 5 27.55 Example 6 25.71 Example 7 26.28 Example 8 22.39 Example 9 30.11 Example 10 31.09 Example 11 18.19 Example 12 19.91 Example 13 21.35 Example 14 25.14 Example 15 28.44 Example 16 29.72 Example 17 30.12 Example 18 28.30 Example 19
(10 ppm) 35.67

As can be seen from the results of Table 3, it was found that the mixed extracts exhibited excellent activity as a natural substance when compared with the representative anti-inflammatory drug L-NMMA, although the relative activity was somewhat low.

<Experimental Example 4> Promoting collagen synthesis

The sample was added to the culture medium of human - derived fibroblasts to confirm the promoting effect of type I collagen synthesis at the cellular level. The synthesized collagen was quantitated using a PICP EIA kit (Procollagen Type I C-Peptide Enzyme Immuno Assay Kit). In order to measure the amount of collagen synthesis, the sample was added to a fibroblast culture medium (DMEM medium) at a final concentration of 10 ppm and cultured for 48 hours. The culture broth was taken and the degree of type 1 collagen synthesis was measured at each concentration using a PICP EIA kit And measured at 450 nm using a spectrophotometer.

For the comparison of the effects, the degree of collagen synthesis was measured in the same manner for the samples in which the culture medium of the untreated fibroblasts (negative control) and vitamin C (positive control) were added to a final concentration of 52.85 / / ml. The increase rate of collagen production was calculated by the ratio of relative collagen production to the negative control, and the results are shown in Table 4 below.

sample Type 1 collagen production (ng / ml) Growth rate (%) Negative control group 150.2 - Positive control (vitamin C) 246.8 64.3 Example 1 201.1 33.89 Example 2 204.5 36.15 Example 3 199.4 32.76 Example 4 191.7 27.63 Example 5 177.3 18.04 Example 6 216.5 44.14 Example 7 193.4 28.76 Example 8 179.9 19.77 Example 9 180.9 20.44 Example 10 189.6 26.23 Example 11 210.1 39.88 Example 12 209.6 39.55 Example 13 213.1 41.88 Example 14 209.9 39.75 Example 15 200.7 33.62 Example 16 215.1 43.21 Example 17 179.5 19.51 Example 18 180.3 20.04 Example 19
(10 ppm) 240.9 60.39

As can be seen from the results in Table 4, the collagen synthesis was increased when the mixed extract was treated, and the collagen synthesis effect was comparable to that of vitamin C, which is generally known to induce collagen synthesis.

<Experimental Example 5> Elastase activity inhibitory effect

The activity inhibitory effect of Elastase, an enzyme that degrades elastin, was confirmed as follows.

Elastase used Elastase from human leukocyte cells and MeOSuc-Ala-Ala-Pro-Val-pNA as synthetic substrate for Elastase. The buffer solution used was 100 mM Tris (pH 7.5) solution. Finally, 0.2 mU was used for the ELASTASES using a buffer solution. In addition, the synthetic substrate of Elastase was diluted with buffer solution to make a final concentration of 0.5 mM by making a 100 mM solution using DMSO. At this time, the positive control group was set to contain 10 ppm of quercetin, which is known as an inhibitor of Elastinase. Ellazease inhibition candidates were added to give a final concentration of 10 ppm. The reaction was carried out in a 96-well plate and allowed to react at room temperature for 20 minutes. Absorbance was measured at 405 nm using a spectrophotometer at intervals of 1 minute, and the slope of the absorbance versus time was determined as the activity of the enzyme. Ella stasis inhibition rates were calculated as follows.

&Quot; (3) "

The inhibition rate of elastase was calculated using Equation (3) and shown in Table 5 below.

sample Enzyme activity Inhibition rate (%) Example 1 5.5 47.12 Example 2 5.9 43.27 Example 3 5.4 48.08 Example 4 5.2 50.00 Example 5 6.6 36.54 Example 6 7.6 26.92 Example 7 6.1 41.35 Example 8 6.4 38.46 Example 9 5.5 47.12 Example 10 5.9 43.27 Example 11 7.9 24.04 Example 12 4.7 54.81 Example 13 7.1 31.73 Example 14 6.6 36.54 Example 15 6.9 33.65 Example 16 4.3 58.65 Example 17 5.1 50.96 Example 18 6.8 34.62 Example 19
(10 ppm) 3.9 62.50 The positive control (Quercetin, 10 ppm) 3.5 66.35 The control (DMSO, 20 ppm) 10.4 -

As can be seen from the results in Table 5, when the mixed extract was treated, it was found that it exhibited a better inhibitory effect on elastase activity, though the effect was somewhat weaker than that of the positive control. Therefore, it was found that the mixed extract can be used for skin regeneration and wrinkle improvement.

<Experimental Example 6> Antioxidant effect

The free radical scavenging ability of the mixed extract was measured by the 1,1-diphenyl-2-picryl hydrazyl (DPPH) method (Blois, Nature 181, 1190, 1958). DPPH is a relatively stable free radical, which shows maximum absorption at 517 nm in the presence of radicals and loses its absorbance when radicals are eliminated. DPPH was purchased from Sigma, and dissolved in methyl alcohol at a concentration of 0.15 mM.

First, 100 쨉 l of the mixed extract or the positive control, vitamin C, was added to each well of a 96-well plate. 100 쨉 l of DPPH solution was added thereto, and the mixture was allowed to stand at room temperature for 30 minutes, and the absorbance at 517 nm was measured using a microplate reader (BioTek EL-340).

IC ₅₀ represents the concentration of the extract when the absorbance of the sample is half the absorbance of the control group. The results are shown in Table 6 below. This experiment was repeated 3 times.

sample IC ₅₀ (% w / v) Control group 0.0025 Positive control (vitamin C) 0.0005 Example 1 0.0022 Example 2 0.0022 Example 3 0.0018 Example 4 0.0016 Example 5 0.0019 Example 6 0.0016 Example 7 0.0019 Example 8 0.0021 Example 9 0.0018 Example 10 0.0018 Example 11 0.0022 Example 12 0.0017 Example 13 0.0022 Example 14 0.0024 Example 15 0.0020 Example 16 00016 Example 17 0.0018 Example 18 0.0021 Example 19 0.0006

As shown in Table 6, the mixed extracts showed a strong free radical scavenging ability as compared with vitamin C, confirming that the antioxidative effect was excellent.

Claims

Extract of Ganoderma lucidum, Extract of Ganoderma lucidum, Extract of Ganoderma lucidum, Extract of Ganoderma lucidum, Extract of Ganoderma lucidum, Extract of Ganoderma lucidum, Extract of Ganoderma lucidum, Extract of Ganoderma lucidum Cosmetic composition for skin whitening, wrinkle improvement, elasticity improvement, trouble improvement, or antioxidation containing an extract and an extract of Phytase as an active ingredient.

The method according to claim 1, wherein the extract of Angelica keiskei: Ginkgo extract: Ganoderma extract: Ganoderma extract: Ganoderma extract: Ganoderma extract: Ganoderma extract: Ganoderma lucidum Extract: 10: 0.01-10: 0.01-10: 0.01-10: 0.01-10: 0.01-10: 0.01-10: 0.01-10: 0.01-10: 0.01-10: Wherein the cosmetic composition is characterized in that the cosmetic composition is 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10: 0.01 to 10.

The cosmetic composition according to claim 1, wherein the extract is extracted with a solvent selected from the group consisting of water, C1 to C4 lower alcohols, and mixtures thereof.

The cosmetic composition according to claim 1, wherein the cosmetic composition is antialceration, inhibition of melanin formation, promotion of collagen synthesis, inhibition of elastase activity or free radical scavenging.