CN110248641A - Skin regeneration, skin comprising silk gum, common cnidium fruit P.E and Herba Visci extract are releived or wound healing composition - Google Patents
Skin regeneration, skin comprising silk gum, common cnidium fruit P.E and Herba Visci extract are releived or wound healing composition Download PDFInfo
- Publication number
- CN110248641A CN110248641A CN201980000271.8A CN201980000271A CN110248641A CN 110248641 A CN110248641 A CN 110248641A CN 201980000271 A CN201980000271 A CN 201980000271A CN 110248641 A CN110248641 A CN 110248641A
- Authority
- CN
- China
- Prior art keywords
- skin
- wound healing
- silk gum
- releived
- cnidium fruit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 93
- 239000000284 extract Substances 0.000 title claims abstract description 81
- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 69
- 241000212948 Cnidium Species 0.000 title claims abstract description 67
- 230000029663 wound healing Effects 0.000 title claims abstract description 49
- 230000036560 skin regeneration Effects 0.000 title claims abstract description 47
- 210000003491 skin Anatomy 0.000 claims abstract description 81
- 239000003814 drug Substances 0.000 claims abstract description 26
- 238000002360 preparation method Methods 0.000 claims abstract description 21
- 230000036541 health Effects 0.000 claims abstract description 19
- 108010014258 Elastin Proteins 0.000 claims abstract description 18
- 235000013376 functional food Nutrition 0.000 claims abstract description 16
- 102000016942 Elastin Human genes 0.000 claims abstract description 15
- 229920002549 elastin Polymers 0.000 claims abstract description 15
- 230000035755 proliferation Effects 0.000 claims abstract description 14
- 230000014509 gene expression Effects 0.000 claims abstract description 13
- 230000003110 anti-inflammatory effect Effects 0.000 claims abstract description 11
- 210000002510 keratinocyte Anatomy 0.000 claims abstract description 11
- 230000005012 migration Effects 0.000 claims abstract description 9
- 238000013508 migration Methods 0.000 claims abstract description 9
- 239000002552 dosage form Substances 0.000 claims description 19
- 239000002537 cosmetic Substances 0.000 claims description 15
- -1 frost Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000003795 chemical substances by application Substances 0.000 claims description 10
- 239000000839 emulsion Substances 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 9
- 230000001815 facial effect Effects 0.000 claims description 7
- 239000003921 oil Substances 0.000 claims description 6
- 239000002674 ointment Substances 0.000 claims description 6
- 239000006210 lotion Substances 0.000 claims description 5
- 210000002540 macrophage Anatomy 0.000 claims description 5
- 239000000344 soap Substances 0.000 claims description 5
- 239000000725 suspension Substances 0.000 claims description 5
- 241001480055 Quercus mongolica Species 0.000 claims description 4
- 239000006071 cream Substances 0.000 claims description 4
- 239000000516 sunscreening agent Substances 0.000 claims description 4
- 239000004094 surface-active agent Substances 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 230000002401 inhibitory effect Effects 0.000 claims description 3
- 239000000686 essence Substances 0.000 claims description 2
- 239000006260 foam Substances 0.000 claims description 2
- 239000011344 liquid material Substances 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 claims description 2
- 230000035764 nutrition Effects 0.000 claims description 2
- 239000006072 paste Substances 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- 230000000475 sunscreen effect Effects 0.000 claims description 2
- 230000002500 effect on skin Effects 0.000 abstract description 10
- 108090000623 proteins and genes Proteins 0.000 abstract description 10
- 239000011159 matrix material Substances 0.000 abstract description 9
- 102000004169 proteins and genes Human genes 0.000 abstract description 6
- 230000001737 promoting effect Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 description 40
- MWUXSHHQAYIFBG-UHFFFAOYSA-N Nitric oxide Chemical compound O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 22
- 238000000034 method Methods 0.000 description 21
- 239000000243 solution Substances 0.000 description 19
- 206010052428 Wound Diseases 0.000 description 17
- 208000027418 Wounds and injury Diseases 0.000 description 17
- 235000014066 European mistletoe Nutrition 0.000 description 15
- 235000012300 Rhipsalis cassutha Nutrition 0.000 description 15
- 241000221012 Viscum Species 0.000 description 15
- 210000004027 cell Anatomy 0.000 description 15
- 238000012790 confirmation Methods 0.000 description 13
- 239000008194 pharmaceutical composition Substances 0.000 description 12
- 239000000047 product Substances 0.000 description 12
- 102000007547 Laminin Human genes 0.000 description 10
- 108010085895 Laminin Proteins 0.000 description 10
- 230000004663 cell proliferation Effects 0.000 description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- 235000013305 food Nutrition 0.000 description 8
- 238000012545 processing Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 206010061218 Inflammation Diseases 0.000 description 6
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 6
- 230000012292 cell migration Effects 0.000 description 6
- 230000006378 damage Effects 0.000 description 6
- 235000019441 ethanol Nutrition 0.000 description 6
- 230000004054 inflammatory process Effects 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 239000002775 capsule Substances 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 235000019198 oils Nutrition 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- 230000000638 stimulation Effects 0.000 description 5
- 230000002195 synergetic effect Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000003085 diluting agent Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 4
- 230000000670 limiting effect Effects 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 239000007921 spray Substances 0.000 description 4
- 239000000375 suspending agent Substances 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 239000000454 talc Substances 0.000 description 4
- 235000012222 talc Nutrition 0.000 description 4
- 229910052623 talc Inorganic materials 0.000 description 4
- 101100447432 Danio rerio gapdh-2 gene Proteins 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 101150112014 Gapdh gene Proteins 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 239000000443 aerosol Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 229920000159 gelatin Polymers 0.000 description 3
- 235000019322 gelatine Nutrition 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 235000011187 glycerol Nutrition 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000003973 paint Substances 0.000 description 3
- 239000000546 pharmaceutical excipient Substances 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 239000003826 tablet Substances 0.000 description 3
- 229940124597 therapeutic agent Drugs 0.000 description 3
- 229910052720 vanadium Inorganic materials 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 201000004624 Dermatitis Diseases 0.000 description 2
- LCGLNKUTAGEVQW-UHFFFAOYSA-N Dimethyl ether Chemical compound COC LCGLNKUTAGEVQW-UHFFFAOYSA-N 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 2
- 229930182566 Gentamicin Natural products 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 229920000168 Microcrystalline cellulose Polymers 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 240000003803 Torilis japonica Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- WNROFYMDJYEPJX-UHFFFAOYSA-K aluminium hydroxide Chemical compound [OH-].[OH-].[OH-].[Al+3] WNROFYMDJYEPJX-UHFFFAOYSA-K 0.000 description 2
- 229910021502 aluminium hydroxide Inorganic materials 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000000440 bentonite Substances 0.000 description 2
- 229910000278 bentonite Inorganic materials 0.000 description 2
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 2
- 235000012241 calcium silicate Nutrition 0.000 description 2
- 239000012930 cell culture fluid Substances 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 235000010980 cellulose Nutrition 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 210000002808 connective tissue Anatomy 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 239000000287 crude extract Substances 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 230000003328 fibroblastic effect Effects 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 229960002518 gentamicin Drugs 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 229940040145 liniment Drugs 0.000 description 2
- 239000000865 liniment Substances 0.000 description 2
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 239000008108 microcrystalline cellulose Substances 0.000 description 2
- 229940016286 microcrystalline cellulose Drugs 0.000 description 2
- 235000019813 microcrystalline cellulose Nutrition 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000006187 pill Substances 0.000 description 2
- 239000010773 plant oil Substances 0.000 description 2
- 239000011505 plaster Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 239000001294 propane Substances 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000037390 scarring Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000037380 skin damage Effects 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 229940032147 starch Drugs 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000001993 wax Substances 0.000 description 2
- 230000037303 wrinkles Effects 0.000 description 2
- 239000011787 zinc oxide Substances 0.000 description 2
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 1
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 1
- AXPZIVKEZRHGAS-UHFFFAOYSA-N 3-benzyl-5-[(2-nitrophenoxy)methyl]oxolan-2-one Chemical compound [O-][N+](=O)C1=CC=CC=C1OCC1OC(=O)C(CC=2C=CC=CC=2)C1 AXPZIVKEZRHGAS-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 241000790917 Dioxys <bee> Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010015150 Erythema Diseases 0.000 description 1
- 239000004386 Erythritol Substances 0.000 description 1
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 description 1
- 208000001034 Frostbite Diseases 0.000 description 1
- 239000001828 Gelatine Substances 0.000 description 1
- 108060003393 Granulin Proteins 0.000 description 1
- 244000130592 Hibiscus syriacus Species 0.000 description 1
- 235000018081 Hibiscus syriacus Nutrition 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 239000004166 Lanolin Substances 0.000 description 1
- 238000000134 MTT assay Methods 0.000 description 1
- 231100000002 MTT assay Toxicity 0.000 description 1
- 229920002774 Maltodextrin Polymers 0.000 description 1
- 239000005913 Maltodextrin Substances 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 235000019483 Peanut oil Nutrition 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Natural products OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- 108010013296 Sericins Proteins 0.000 description 1
- 229910003978 SiClx Inorganic materials 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241001530746 Viscaceae Species 0.000 description 1
- 241000221013 Viscum album Species 0.000 description 1
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000001741 anti-phlogistic effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 239000007767 bonding agent Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 239000001273 butane Substances 0.000 description 1
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000001465 calcium Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000009028 cell transition Effects 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000015218 chewing gum Nutrition 0.000 description 1
- 229940112822 chewing gum Drugs 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- KYKAJFCTULSVSH-UHFFFAOYSA-N chloro(fluoro)methane Chemical compound F[C]Cl KYKAJFCTULSVSH-UHFFFAOYSA-N 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 235000008504 concentrate Nutrition 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 239000002385 cottonseed oil Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 231100000429 cutaneous necrosis Toxicity 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003405 delayed action preparation Substances 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000006196 drop Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 description 1
- 235000019414 erythritol Nutrition 0.000 description 1
- 229940009714 erythritol Drugs 0.000 description 1
- 150000002194 fatty esters Chemical class 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 239000007903 gelatin capsule Substances 0.000 description 1
- 238000001879 gelation Methods 0.000 description 1
- 239000003349 gelling agent Substances 0.000 description 1
- CEAZRRDELHUEMR-UHFFFAOYSA-N gentamicin Chemical compound O1C(C(C)NC)CCC(N)C1OC1C(O)C(OC2C(C(NC)C(C)(O)CO2)O)C(N)CC1N CEAZRRDELHUEMR-UHFFFAOYSA-N 0.000 description 1
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 1
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000003862 health status Effects 0.000 description 1
- 210000001320 hippocampus Anatomy 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000027866 inflammatory disease Diseases 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- SUMDYPCJJOFFON-UHFFFAOYSA-N isethionic acid Chemical compound OCCS(O)(=O)=O SUMDYPCJJOFFON-UHFFFAOYSA-N 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 229940039717 lanolin Drugs 0.000 description 1
- 235000019388 lanolin Nutrition 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000005461 lubrication Methods 0.000 description 1
- 235000019359 magnesium stearate Nutrition 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 239000000845 maltitol Substances 0.000 description 1
- VQHSOMBJVWLPSR-WUJBLJFYSA-N maltitol Chemical compound OC[C@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O VQHSOMBJVWLPSR-WUJBLJFYSA-N 0.000 description 1
- 235000010449 maltitol Nutrition 0.000 description 1
- 229940035436 maltitol Drugs 0.000 description 1
- 229940035034 maltodextrin Drugs 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 229960001855 mannitol Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 235000010981 methylcellulose Nutrition 0.000 description 1
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 239000012046 mixed solvent Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- CQDGTJPVBWZJAZ-UHFFFAOYSA-N monoethyl carbonate Chemical compound CCOC(O)=O CQDGTJPVBWZJAZ-UHFFFAOYSA-N 0.000 description 1
- DILRJUIACXKSQE-UHFFFAOYSA-N n',n'-dimethylethane-1,2-diamine Chemical compound CN(C)CCN DILRJUIACXKSQE-UHFFFAOYSA-N 0.000 description 1
- IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 description 1
- OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 239000000312 peanut oil Substances 0.000 description 1
- WVDDGKGOMKODPV-ZQBYOMGUSA-N phenyl(114C)methanol Chemical compound O[14CH2]C1=CC=CC=C1 WVDDGKGOMKODPV-ZQBYOMGUSA-N 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 235000014786 phosphorus Nutrition 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 235000019422 polyvinyl alcohol Nutrition 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 239000003380 propellant Substances 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001603 reducing effect Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 210000004706 scrotum Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000008159 sesame oil Substances 0.000 description 1
- 235000011803 sesame oil Nutrition 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 230000037394 skin elasticity Effects 0.000 description 1
- 239000008234 soft water Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 235000010215 titanium dioxide Nutrition 0.000 description 1
- 208000004371 toothache Diseases 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 210000003905 vulva Anatomy 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 235000014692 zinc oxide Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/318—Foods, ingredients or supplements having a functional effect on health having an effect on skin health and hair or coat
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Mycology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Nutrition Science (AREA)
- Birds (AREA)
- Botany (AREA)
- Epidemiology (AREA)
- Food Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention provide a kind of skin regeneration comprising silk gum, common cnidium fruit P.E and Herba Visci extract with, wound healing with or skin releive with composition, it releives in particular to a kind of skin regeneration comprising silk gum, common cnidium fruit P.E and Herba Visci extract, skin or wound healing applies some make up composition, skin preparations for extenal use, the outer product composition of medicine and health functional food composition.The proliferation and migration of silk gum, common cnidium fruit P.E and Herba Visci extract of the invention due to promoting keratinocyte, promote the expression of the elastin laminin as the protein for constituting dermal matrix, and show inhibit NO generate anti-inflammatory activity, therefore skin regeneration can be effectively applied to, skin is releived and wound healing in.
Description
Technical field
The present invention relates to it is a kind of comprising silk gum (sericin), common cnidium fruit P.E and Herba Visci extract skin regeneration,
Skin is releived or wound healing composition, extracts in particular to one kind comprising silk gum, common cnidium fruit P.E and mistletoe
Skin regeneration, the skin of object are releived or wound healing applies some make up composition, skin preparations for extenal use, the outer product composition of medicine and guarantor
Health-care function food compositions.
Background technique
Skin and external environment undertake the function that our body is protected from a variety of injurious factors while directly contact.
But skin is damaged because of the increase at age, ultraviolet light, external contamination substance, pressure, wound etc., start in this case by
Damage the regenerative process of skin.Skin regeneration is organized renewing process, via including cell Proliferation, differentiation and duplication, matrix egg
Complex biological process and structure including the synthesis of white (matrix protein), the reconstructing etc. of the new life of blood vessel and wound
At.
Specifically, generating inflammatory reaction first if skin damage.Because by preying on the bacterium entered through wound
The macrophage of activation starts inflammatory reaction, in order to make such as NO, NO2、HNO2、ONOO-Deng active nitrogen species (reactive
Ntrogen species, RNS) attack foreign matter, largely generate inflammatory reaction.But since excessive inflammatory reaction is given birth to too much
At above-mentioned active nitrogen species and cause cutaneous necrosis, tubercle, tumour etc., therefore needs to adjust scorching to carry out treatment appropriate
Disease reaction.
Then, cell Proliferation and transition process are carried out, the process is lasting from after skin damage two days to be carried out to three weeks.
Cell after the period fibroblast active proliferation, proliferation is mobile towards damaged skin and correction of the defect, or accumulation glue
The stromatins such as former albumen, elastin laminin and assign elastic force, moisturizing, the flexibility of skin etc..Therefore, for skin regeneration, wound
Mouthful healing and skin stimulation alleviation and suitable control inflammatory reaction, and by promote it is fibroblastic proliferation and migration etc.
And it is critically important for reconstructing skin.
Related to thisly, it carrying out the regeneration for skin, releiving or the exploitation of many kinds of substance of wound healing.Tool
For body, it is known to the use for containing the Hippocampus extract for showing effect for promoting collagen to generate etc. as effective component
In cosmetic composition (the Korean Patent Publication No. 10- that moisturizing, wrinkle improvement, whitening, antiallergy or skin are releived
No. 1788537), comprising the rose of Sharon tree from the effect etc. for showing the proliferation and migration that promote keratinocyte spend middle extraction
The external preparation and cosmetic composition (Korean Patent Publication No. 10-1762750) for skin regeneration of essential oil etc..
Summary of the invention
Technical problem
The present inventor a kind of make skin regeneration or releives and show the substance of wound healing effect and carry out to develop
Further investigation, result confirm the mixture of silk gum, common cnidium fruit P.E and Herba Visci extract promote keratinocyte/
Fibroblastic proliferation and migration, and promote to constitute the expression of the gene of dermal matrix, and then show excellent anti-inflammatory
Disease effect, so as to complete the present invention.
Technical solution
A purpose of the invention is to provide a kind of skin comprising silk gum, common cnidium fruit P.E and Herba Visci extract again
Raw, skin is releived or wound healing applies some make up composition.
It is a further object of the present invention to provide a kind of skins comprising silk gum, common cnidium fruit P.E and Herba Visci extract again
Life, skin is releived or wound healing skin preparations for extenal use.
It is yet another object of the invention to provide a kind of skins comprising silk gum, common cnidium fruit P.E and Herba Visci extract again
Raw, skin is releived or the outer product composition of wound healing medicine.
It is yet another object of the invention to provide a kind of skins comprising silk gum, common cnidium fruit P.E and Herba Visci extract again
Life, skin is releived or wound healing health functional food composition.
Beneficial effect
Silk gum, common cnidium fruit P.E and Herba Visci extract of the invention is due to promoting the proliferation of keratinocyte and moving
It moves, promotes the expression of the elastin laminin as the protein for constituting dermal matrix, and show the anti-inflammatory work for inhibiting NO to generate
Property, thus the regeneration of skin can be effectively applied to, releive and wound healing in.
Detailed description of the invention
Fig. 1 is the cell Proliferation facilitation effect for showing the mixture of silk gum, common cnidium fruit P.E and Herba Visci extract
Chart.Wherein, * indicates that p < 0.05, * * indicate P < 0.01.
Fig. 2 is the cell migration facilitation effect for showing the mixture of silk gum, common cnidium fruit P.E and Herba Visci extract
Chart (A) and image (B).Wherein, * indicates that p < 0.05, * * indicate P < 0.01.
Fig. 3 is the dermal matrix gene for showing the mixture of silk gum, common cnidium fruit P.E and Herba Visci extract
(elastin；Elastin laminin) expression facilitation effect chart.Wherein, * indicates p < 0.05.
Fig. 4 be show the mixture of silk gum, common cnidium fruit P.E and Herba Visci extract NO (Nitric Oxide,
Nitric oxide) generate the chart for reducing effect.Wherein, * indicates p < 0.05.
Specific embodiment
A mode of the invention provide a kind of skin regeneration comprising silk gum, common cnidium fruit P.E and Herba Visci extract,
Skin is releived or wound healing applies some make up composition.
Silk gum, common cnidium fruit P.E and Herba Visci extract of the invention is due to promoting the proliferation of keratinocyte and moving
It moves, the anti-inflammatory excellent effect for promoting to constitute the expression of the elastin gene of dermal matrix, and NO being inhibited to generate, therefore energy
Show excellent skin regeneration, skin is releived or wound healing effect.
Term " silk gum " of the invention is the protein made of silkworm, shows the characteristic similar with gelatin.If will
The silk gum is dissolved in cooling then gelation after hot water, since dissolubility of the silk gum to water is higher and the lower and easy use of molecular weight
Make extract.
Term " frutus cnidii " (Erect hedge parsley) of the invention, which refers to, to be belonged to Torilis japonica
The umbelliferous biennial herbaceous plant of scientific name.The frutus cnidii is used as medicinal material in Chinese medicine, it is known that chronic eczema or vulva
The pruritus in portion, eczema of scrotum are effective.
Term " mistletoe " (mistletoe) of the invention refers to posting for the Viscaceae with Viscum album scientific name
Raw shrub.The stem and leaf of the mistletoe are used as medicinal material in Chinese medicine, it is known that in toothache, pain in the loins, frostbite, artery sclerosis etc.
Use the mistletoe.
It is posted in addition, term " common cnidium fruit P.E " of the invention and " Herba Visci extract " refer to from the frutus cnidii and Mongolian oak
The extract extracted in life.
Specifically, the term " extract " includes to be obtained and extracting processing to the frutus cnidii or mistletoe
To extracting solution, the extracting solution dilution or concentrate, the dried object as obtained from being dried the extracting solution,
Thick purified or purified or their mixture of the extracting solution etc., extracting solution itself and can using extracting solution come
The extract of all dosage forms formed.
The method for extracting the frutus cnidii or mistletoe is not particularly limited, can be according to usually used in the art
Method extracts the frutus cnidii or mistletoe.As the non-limiting example of the extracting method, hot water extraction method can be enumerated, surpassed
Sound wave extraction method, filtration method, reflux extraction etc., these methods can be individually performed or and be held with two or more methods
Row.
In addition, the type for extracting the Extraction solvent used when the frutus cnidii or mistletoe is not particularly limited, can be used
Any solvent well known in the art.As the non-limiting example of the Extraction solvent, water, carbon atom number 1 can be enumerated
To 4 alcohol or their mixed solvent etc., they can be used alone or mix more than one come using.
Term " skin regeneration " of the invention refers to damage for corresponding to that whole skin aging and skin induce etc. and again structure
At the series reaction of skin histology, skin is mainly executed by the proliferation and migration of the Skin Cell of keratinocyte etc.
Regeneration.Skin elasticity increase and effect improving wrinkles can be showed with skin regeneration.In addition, the damage can be because of purple
Caused by outside line, external contamination substance, wound, wound, burn, pressure etc., but it is not limited to this.
Term " wound " of the invention is the state that living body is damaged, also referred to as wound.It can be because of ultraviolet light, external contaminants
The internal causes such as the external causes such as matter, wound, wound, burn or pressure and cause the damage.
In addition, term " wound healing " of the invention refers to the recovery of wound as described above.The agglutination is drawn
It is divided into acute stage, reparation phase and scarring step.Acute stage, is also referred to as the exudation phase, with inflammatory reaction and blood coagulation reaction.
The reparation phase is also referred to as proliferation period, the proliferation and migration active progression of cell, and passes through one of actively synthesis as connective tissue
Kind the protein such as collagen, elastin laminin and regenerate epidermal tissue.Scarring step be active cell Proliferation it is slack-off and
The step of physical strength of damage location increases while crosslinking constitutes the protein of connective tissue, not with the normal tissue of surrounding
Its same hetero-organization is located at damage location.It is cicatrized a wound and step as described above repeatedly.
In the present invention, the skin regeneration or the reason of wound healing, are not limited, but can specifically pass through promotion
The skin regeneration or wound healing are realized in proliferation, migration or the expression of elastin gene of keratinocyte.
Term " skin is releived " of the invention refers to alleviation and subside erythema or stimulated skin part etc., can be packet
Concept including alleviating containing skin irritatin.For example, the moisture loss reduction and/or blush reduction of skin can be enumerated, but and unlimited
In this.
In the present invention, skin reason of releiving is not limited, and specifically can realize that skin is releived by anti-inflammatory activity,
And can the anti-inflammatory activity of composition through the invention soothed the skin to improve scytitis.
In a specific embodiment of the invention, the mixture of confirmation silk gum, common cnidium fruit P.E and Herba Visci extract
Promote the proliferation and migration (Fig. 1 and Fig. 2) of keratinocyte compared with each monomeric substance, and promotes as dermal matrix
The gene expression (Fig. 3) of the elastin laminin of albumen shows the antiphlogistic effects (Fig. 4) for inhibiting LPS induction NO synthesis.
These give the compositions of the invention comprising the silk gum, common cnidium fruit P.E and Herba Visci extract can be effective
Ground is used as skin regeneration, wound healing or skin and releives the enlightenment of purposes.
Cosmetic composition of the invention relative to the total weight of composition may include 0.0001 to 80 weight %, it is specific and
Say that 0.0005 to 70 weight %, more specifically the silk gum, common cnidium fruit P.E and the mistletoe of 0.001 to 60 weight % mention
Take object.If the content less than 0.0001 weight %, is unable to fully realize that desired activity is without preferred originally, if
The content is greater than 80 weight %, then can not correspondingly expect the enhancing of apparent effect with increased content and noneconomic,
And it is possible that problem occurs in terms of the stability of cosmetic composition.
Cosmetic composition according to the present invention can be prepared as selecting free solution, externally-applied ointment, frost, foam, nutrient laden
Adornment water, soft toner, facial mask, soft water, lotion, sun screen, essence, perfumed soap, cleaning liquid material, balneation agent, suncream,
Sunblock lotion, suspension, emulsion, paste, gel, dew, powder, perfumed soap, the facial cleanser containing surfactant, oil, powder foundation, cream
Turbid foundation cream, wax powder bottom, pad pasting and the group formed by spraying dosage form, but it is not limited to this.
In addition, cosmetic composition of the invention can further include the cosmeceutical being formulated in common skin cosmetics
More than one upper receivable carrier, can suitably deploy as such as oil of usual ingredient, water, surfactant, guarantor
Humectant, lower alcohol, thickener, chelating agent, pigment, preservative, fragrance etc., but it is not limited to this.
Include on the cosmeceutical in cosmetic composition of the invention receivable carrier it is a variety of more according to dosage form
Sample.
In the case where dosage form of the invention is ointment, paste, frost or gel, animality can be utilized as carrier components
Oil, plant oil, wax, paraffin, starch, bassora gum, cellulose derivative, polyethylene glycol, organosilicon, bentonite, silica,
Talcum, zinc oxide or their mixture.
In the case where dosage form of the invention is pulvis or spray, lactose, talcum, dioxy can be utilized as carrier components
SiClx, aluminium hydroxide, calcium silicates, Silon or their mixture, especially in the feelings that dosage form of the invention is spray
Under condition, the propellant of such as hydrogen chlorofluorocarbon, propane/butane or dimethyl ether can further include.
Dosage form of the invention be solution or emulsion in the case where, as carrier components can using solvent, solubilizer or
Emulsifier, for example, using water, ethyl alcohol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, Ergol, propylene glycol and
1,3-BDO oil, especially using cottonseed oil, peanut oil, maize germ oil, olive oil, castor oil and sesame oil, glycerol
The aliphatic ester of fatty ester, polyethylene glycol or sorbitan.
In the case where dosage form of the invention is suspension, liquid diluent (such as water, ethyl alcohol can be utilized as carrier components
Or propylene glycol), suspending agent (such as ethoxylated isostearyl alcohols, polyoxyethylene sorbitan ester and polyoxyethylene sorbitan
Ester), microcrystalline cellulose, inclined aluminium hydroxide, bentonite, agar or bassora gum etc..
In the case where dosage form of the invention is perfumed soap, alkali metal salt, the fat of fatty acid can be utilized as carrier components
Sour half ester salt, fatty acid protein hydrolysate, isethionate, lanolin derivative, aliphatic alcohol, plant oil, glycerol, sugar
Deng.
In the case where dosage form of the invention is facial mask (removing facial mask, cleaning (wash off) facial mask or mask sheet), make
Polyvinyl alcohol, kaolin, talcum, zinc oxide or titanium dioxide etc. can be utilized for carrier components.
Another way of the invention provides a kind of mixture comprising silk gum, common cnidium fruit P.E and Herba Visci extract
Skin regeneration, skin are releived or wound healing skin preparations for extenal use.
At this point, about " silk gum ", " common cnidium fruit P.E ", " Herba Visci extract ", " skin regeneration ", " wound is cured
Conjunction " and the explanation of " skin is releived " are as previously described.
Term " skin preparations for extenal use " of the invention is comprising generally the one of entire substance encompassing used in external preparation for skin
Concept there is plaster (plaster), paint (lotion), liniment as the non-limiting example of skin preparations for extenal use dosage form
(liniment), liquid preparation (liquid and solution), aerosol (aerosol), extract (extracts), soft
Paste (ointment), liquid extract (fluid extracts), emulsion (emulsion), suspending agent (suspesions), glue
Wafer (capsules), creme (creams), soft gelatin capsule, hard gelatine capsule, patch or sustained release preparation.
Skin preparations for extenal use according to the present invention can be by adding common inorganic or organic carrier, excipient and dilute
Release agent and preparation turns to the non-oral administration agent of solid, semisolid or liquid form.It can for the para-oral preparation
To be the percutaneous dosing in the group being made of drops, ointment, paint, gel, frost, patch, spray, suspending agent and emulsion
Type dosage form, but it is not limited to this.
As may include carrier, excipient and diluent in the external preparation, can enumerate lactose, glucose, sucrose,
Oligosaccharide, D-sorbite, mannitol, xylitol, erythritol, maltitol, starch, Arabic gum, alginates, gelatin, phosphorus
Sour calcium, calcium silicates, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methyl hydroxybenzoate, oxybenzene propane
Ester, talcum, magnesium stearate and mineral oil.
For the Dermatologic preparation composition using each dosage form, those skilled in the art can be according to other external preparation for skin
The dosage form of agent or suitably selected without difficulty using purpose and cooperate in addition to the composition of aforementioned present invention other at
Point, it is possible to generate the effect that is multiplied when applying simultaneously with other raw materials in this case.
In addition, skin preparations for extenal use of the invention can also be used as pharmaceutical compositions use.
Pharmaceutical compositions of the invention can further include and usually used in the preparation of pharmaceutical compositions can pharmaceutically connect
Carrier, excipient or the diluent of receipts, the carrier may include non-natural carrier (non-naturally occuring
carrier)。
The pharmaceutical compositions can turn to powder, granule, tablet, capsule, suspension by each usual method dosage form
The oral type dosage form of liquid, emulsion agent, syrup or aerosol etc., external preparation, suppository and sterilizing injecting solution form and use.
" pharmaceutical acceptable ", which refers to, to be showed and will not have to the cell being exposed in the composition or the mankind
Virose characteristic.
Specifically, the type of the carrier is not particularly limited, as long as usually used in the technical field and pharmacy
Any carrier may be used in upper receivable carrier.As the non-limiting example of the carrier, salt water, aqua sterilisa, woods can be enumerated
Grignard solution, buffered saline, albumin injection solution, glucose solution, maltodextrin solution, glycerol, ethyl alcohol etc..They can be independent
Using or mixing it is two or more and use.In addition, addition antioxidant, buffer and/or bacteriostat etc. can be passed through when necessary
Other common additives and use, and can be by further adding diluent, dispersing agent, surfactant, bonding agent, profit
Lubrication prescription etc. come preparation turn to such as aqueous solution, suspension, the injectable dosage forms of emulsion, pill, capsule, particle or tablet etc. and
It uses.
At this point, include the content of silk gum in the pharmaceutical compositions, common cnidium fruit P.E and Herba Visci extract not
It is particularly limited, relative to the total weight of pharmaceutical compositions, may include 0.01 to 100 weight %, specifically may include 1 to 80
Weight %.
Another mode of the invention provides that a kind of skin regeneration, skin is releived or wound healing method, and this method includes will
The pharmaceutical compositions are administered into the step in the individual for needing the pharmaceutical compositions.
At this point, about " silk gum ", " common cnidium fruit P.E ", " Herba Visci extract ", " skin regeneration ", " wound is cured
Conjunction " and the explanation of " skin is releived " are as previously described.
Term " individual " of the invention refer to comprising need skin regeneration, skin releive or the mankind of wound healing including
All animals.
Term " administration " of the invention, which refers to, imports defined substance to individual using method appropriate.It can be by that can arrive
Composition of the invention is administered up to any general path of intravital target.The administration routes of the composition of the application not by
Especially limitation, can carry out oral or non-oral administration.Specifically, non-oral administration can be carried out, it more specifically can be using painting
Spread on the mode (that is, percutaneous dosing) of skin.
More specifically, skin regeneration of the invention, skin are releived or wound healing method may include to individual with pharmacy
The step of pharmaceutical compositions are administered in upper effective amount.
Term of the invention " pharmaceutically effective amount " refers to be enough to treat by medicine and pharmacology in it is applicable it is reasonable be benefited/
Risk-benefit risks treats illness and will not cause the amount of the degree of side effect, and those skilled in the art can be according to the property comprising patient
Not, when age, weight, health status, kinds of Diseases, severe degree, pharmaceutical activity, the susceptibility to drug, medication, administration
Between, administration routes and discharge ratio, the element during treatment, including allotment or the drug that uses simultaneously and other medical domains
In well-known element be easy to determine that effective dose is horizontal.
Pharmaceutical compositions of the invention can be administered with independent therapeutic agents, be perhaps administered together with other therapeutic agents or
Pharmaceutical compositions and existing therapeutic agent of the invention can sequentially or simultaneously be administered.Furthermore, it is possible to which singly one or more sheets are administered
The pharmaceutical compositions of invention.Consider above-mentioned all elements and being administered while will not inducing side effect can be obtained most with minimum
The amount of big effect be it is critically important, this can be easy to determine by those skilled in the art.
Another mode of the invention provides a kind of skin comprising silk gum, common cnidium fruit P.E and Herba Visci extract again
Raw, skin is releived or the outer product composition of wound healing medicine.
At this point, about " silk gum ", " common cnidium fruit P.E ", " Herba Visci extract ", " skin regeneration ", " wound is cured
Conjunction " and the explanation of " skin is releived " are as previously described.
Term of the invention " the outer product of medicine " refers to and with diagnosis, treatment, improvement, mitigation, disposition or the prevention mankind or moves
The pharmaceuticals in article that the purpose of the disease of object the uses article small compared to effect.For example, medicine involved in medicine thing method
Outer product are the productions other than the article that the purposes with pharmaceuticals uses, comprising the disease for treating or preventing humans/animals
Product, to small or product that will not directly act on of effect of human body etc..
Silk gum according to the present invention, common cnidium fruit P.E and Herba Visci extract are being used as the outer product additive of medicine
In the case of, it can directly add the composition or can be used together with product outside other medicine or the outer product ingredient of medicine described
Composition can suitably use the composition according to usual method.Can according to use purpose (prevention, health or therapeutic treatment) come
Suitably determine the combined amount of effective component.
The outer product composition of medicine is not particularly limited, specifically, can be by being prepared as ointment, paint, being sprayed
Agent, patch agent, creme, powder, suspending agent, gelling agent or gel form come using.
Another mode of the invention provides a kind of mixture comprising silk gum, common cnidium fruit P.E and Herba Visci extract
Skin regeneration, skin are releived or wound healing health functional food composition.
At this point, about " silk gum ", " common cnidium fruit P.E ", " Herba Visci extract ", " skin regeneration ", " wound is cured
Conjunction " and the explanation of " skin is releived " are as previously described.
Term " health functional food " (functional food) of the invention be and specific health food (food
For special health use, FoSHU) identical term, refer to be processed as efficiently presenting in addition to supplying nutrition and live
The high food of the medicine and medical effect of body regulative mechanism.In the present invention, the health functional food and health food or guarantor
Strong accesary foods are mixed.
Health functional food composition of the invention can be absorbed due to daily, can expect that skin regeneration, skin relax
Slow or wound healing effect, and can be combined highly usefully using health functional food of the invention with health enhancing purpose
Object.
At this point, including silk gum, common cnidium fruit P.E and the Herba Visci extract in the health functional food composition
Content be not particularly limited, may include 0.01 to 100 weight % relative to the total weight of health functional food composition, tool
It may include 1 to 80 weight % for body.
The health functional food can be turned to by dosage form selected from by pill, powder, particle, electuary, tablet, capsule and drink
The group of material form composition and prepare, releived to skin regeneration, skin or effect that wound healing is useful with that can obtain.In addition,
To the type of the health functional food without other limitations, such as to can be various beverages, chewing gum, tea, vitamin compound
Agent, health care accesary foods class etc..
The prevention or improvement that will not interfere health functional food can be added in the health functional food composition
Other compositions, type is not particularly limited.For example, being used as adding ingredient that can contain various Chinese medicines as common food
Receivable food auxiliary additive or natural carbohydrate etc. on extract, bromatology.
In addition, the health functional food composition can further include receivable carrier in bromatology or can lead to
The ingredient for being commonly used for food compositions and improving smell, taste, vision etc., this can suitably be selected by those skilled in the art
To use.
Another mode of the invention provides a kind of composition comprising silk gum, common cnidium fruit P.E and Herba Visci extract
It releives for skin regeneration, skin or the purposes of wound healing.Another mode of the invention provides a kind of comprising silk gum, frutus cnidii
The composition of extract and Herba Visci extract be used to prepare skin regeneration, skin is releived or wound healing applies some make up, is eaten
The purposes of product, pharmaceuticals or medicament.
It is as previously described about composition comprising the silk gum, common cnidium fruit P.E and Herba Visci extract and application thereof.
Embodiment
In the following, by following examples, the present invention is described in detail.But following examples are only intended to illustrate this hair
It is bright, it is limited the scope of the invention not by following examples.
Preparation example 1: the acquisition methods of silk gum, common cnidium fruit P.E and Herba Visci extract
Firstly, obtaining silk gum using method as described below.Firstly, using the silk cocoon after cutting as raw material come using.
7~8 times of water (H is added relative to quantitative raw material weight2O), and utilize steam to improve temperature and be mentioned at 95 DEG C
It takes six hours and obtains silk gum crude extract.Later, the crude extract is filtered using 20 μm of filters, and to the mistake
Protein decomposition enzyme is added in filtrate and is reacted 24 hours at 55 DEG C, heats 30 points at 95 DEG C to inactivate enzyme reaction
Clock.Then, concentrated filtrate and powdered is carried out using spray dryer after being filtered using 10 μm of filters.
In addition, obtaining Herba Visci extract using method as described below.After washing and dry mistletoe, to Mongolian oak
In parasitic 50g be added butanediol (Butylene Glycol) 400g and water 550g and at 50 DEG C using ultrasonic extractor come
It extracts 72 hours.Then, maturation in 24 hours is carried out, and utilizes 0.3 μm of filter after being filtered using 0.5 μm of filter
It is filtered to obtain each extract.
In addition, obtaining common cnidium fruit P.E using method as described below.Frutus cnidii 1kg after quantitative drying is simultaneously washed
After washing, distilled water 49kg is added and is extracted 2 hours at 60 DEG C.After maturation extracts at room temperature liquid phase 48 hours,
It is filtered using filter-press (Filter press).Filtered extracting solution is removed using sterilizing filter
Bacterium processing, and anti-corrosion raw material is added to prepare common cnidium fruit P.E.
Embodiment 1: the cell Proliferation facilitation effect of the mixture of confirmation silk gum, common cnidium fruit P.E and Herba Visci extract
In order to confirm that the mixture of silk gum, common cnidium fruit P.E and Herba Visci extract is releived to skin regeneration, skin, pierced
The effect for swashing alleviation, wound healing etc. confirms that the cell Proliferation of the mixture promotes by MTT analysis (MTT assay)
Effect.
Specifically, DMEN (high sugar) culture medium of the gentamicin (gentamicine) in addition 10%FBS and 1%
In with 37 DEG C, 5%CO2HaCat of the CMC model as Human keratinocytes (Human keratinocyte cell).
Then, by HaCat cell in 96 orifice plates plant division at 5 × 103The quantity of cells/well, and with individual silk gum (0.001%,
V/v), individual common cnidium fruit P.E (0.002%, v/v), individual Herba Visci extract (0.001%, 0.002%,
0.005%, 0.01%, v/v) or their mixture handled after cultivate 24 hours.It then, is 100 by the processing of MTT solution
The concentration of μ g/mL, and in 37 DEG C, 5%CO2Culture solution is removed after reacting in culture medium one hour, in each hole respectively
Acidity-isopropanol (acid-isopropanol) 100 μ l is added and measures absorbance using ELISA reader at 570nm.
[table 1]
As a result, confirmation as shown in above-mentioned table 1 and Fig. 1, is extracted with individual silk gum, common cnidium fruit P.E or mistletoe
The case where object processing, is compared, in the case where being handled with their mixture ability of cell proliferation increase to about 1.3 to 1.5 times with
On.In particular, know do not have ability of cell proliferation when although common cnidium fruit P.E and Herba Visci extract are used alone,
Excellent synergistic effect can be played in the case where being used in mixed way with silk gum.
By the above results it is found that silk gum, common cnidium fruit P.E and Herba Visci extract mixture and they individually make
Promote activity significant brilliant with compared to cell Proliferation, thus releive to skin regeneration, skin, stimulation is alleviated, wound healing etc. is in
Reveal excellent effect.
In addition, confirmation Herba Visci extract all has excellent ability of cell proliferation at various concentrations, even if concentration is not
It is same also to show synergistic effect.
Embodiment 2: the cell migration facilitation effect of the mixture of confirmation silk gum, common cnidium fruit P.E and Herba Visci extract
In order to confirm that the mixture of silk gum, common cnidium fruit P.E and Herba Visci extract is releived to skin regeneration, skin, pierced
The effect for swashing alleviation, wound healing etc., confirms the mixture by wound healing analysis (Wound healing assay)
Cell migration facilitation effect.
Specifically, by HaCat cell in 96 orifice plates plant division at 1 × 103The quantity of cells/well, and at 24 hours
Replace with later addition 5%FBS DMEM culture medium, then the individual silk gum of each Kong Zhongyong (0.0005%, 0.002%, v/
V), individual common cnidium fruit P.E (0.002%, 0.01%, v/v), individual Herba Visci extract (0.0005%,
0.005%, v/v) or their mixture handled.Wound is supplied to using the yellow tip (yellow tip) after 48 hours
Mouthful, and the degree for wound healing of taking pictures at 24 hours later and by image J (image J) program come measurement area.
[table 2]
As a result, confirmation as shown in above-mentioned table 2 and Fig. 2, is extracted with individual silk gum, common cnidium fruit P.E or mistletoe
The case where object processing, is compared, in the case where being handled with their mixture cell migration ability increase to about 1.3 to 1.8 times with
On.
By the above results it is found that silk gum, common cnidium fruit P.E and Herba Visci extract mixture and they individually make
Promote activity significant brilliant with compared to cell migration, thus releive to skin regeneration, skin, stimulation is alleviated, wound healing etc. is in
Reveal excellent effect.
In addition, confirmation common cnidium fruit P.E or Herba Visci extract all have excellent cell migration energy at various concentrations
Power shows synergistic effect concentration difference.
Embodiment 3: the table of the dermal matrix gene of the mixture of confirmation silk gum, common cnidium fruit P.E and Herba Visci extract
Up to facilitation effect
In order to confirm that the mixture of silk gum, common cnidium fruit P.E and Herba Visci extract is releived to skin regeneration, skin, pierced
The effect for swashing alleviation, wound healing etc., confirms the mixture to the elastic egg as dermal matrix gene by RT-PCR
The expression facilitation effect of white (elastin).
Specifically, with 37 DEG C, 5%CO in FGM-2Bullet kit (Bullet kit) culture medium2CMC model
NHDF as human dermal fibroblasts (normal human dermal fibroblast).Then, by NHDF cell
Plant division is at 1 × 10 in 6 orifice plates5The quantity of cells/well, and the individual silk gum of each Kong Zhongyong (0.002%, 0.005%,
V/v), individual common cnidium fruit P.E (0.005%, v/v), individual Herba Visci extract (0.002%, 0.01%, v/v) or
Their mixture is handled.It is dissolved at 24 hours later using Trizol and extracts total serum IgE after cell, and should
Total serum IgE is converted to the expression quantity that cDNA confirms elastin gene by qPCR later.At this point, the used following institute of primer
It states.Elastin laminin (F:5'-TCG CAA CAC GTA ACG CAA T-3', sequence number 1；R:5'-ACT TCT CTT TTT
CCA CCC CAT TT-3', sequence number 2), gapdh (glyceraldehyde-3-phosphate dehydrogenase) (F:5'-CCT CAA GAT CAT
CAG CAA T-3', sequence number 3；R:5'-CCA TCC ACA GTC TTC TGG GT-3', sequence number 4).
[table 3]
As a result, confirmation as shown in above-mentioned table 3 and Fig. 3, is extracted with individual silk gum, common cnidium fruit P.E or mistletoe
The case where object processing, is compared, and in the case where being handled with their mixture, the elastin laminin expression quantity of cell increases to about 1.3
To 2 times or more.
By the above results it is found that silk gum, common cnidium fruit P.E and Herba Visci extract mixture and they individually make
Promote activity significant brilliant with the elastin gene expression compared to cell, thus releive to skin regeneration, skin, stimulation is delayed
Solution, wound healing etc. show excellent effect.
In addition, confirmation silk gum or Herba Visci extract all have excellent elastin laminin expression at various concentrations promotes effect
Fruit shows synergistic effect concentration difference.
Embodiment 4: the anti-inflammatory effect of the mixture of confirmation silk gum, common cnidium fruit P.E and Herba Visci extract
In order to confirm that the mixture of silk gum, common cnidium fruit P.E and Herba Visci extract is releived to skin regeneration, skin, pierced
The effect for swashing alleviation, wound healing etc., the anti-inflammatory of the mixture is confirmed by NO (Nitric Oxide) synthesis degree
Effect.
Specifically, with 37 in DMEM (High glucose) culture medium of the gentamicin of addition 10%FBS and 1%
DEG C, 5%CO2Raw264.7 of the CMC model as mouse macrophage (murine macrophage).Then, by Raw264.7
Cell in 12 orifice plates plant division at 5 × 105The quantity of cells/well, and with 1 μ g/mL of LPS and individual silk gum (0.002%,
V/v), individual common cnidium fruit P.E (0.001%, 0.002%, v/v), individual Herba Visci extract (0.0005%,
0.001%, v/v) or their mixture handled after cultivate 24 hours.Then, aggregation cell culture fluid and carry out from
React 100 μ l of cell culture fluid 100 μ l and Ge Lisi (Griess) reagent ten minutes after heart separation, and at 540nm
Absorbance is measured using ELISA reader.
[table 4]
As a result, confirmation as shown in above-mentioned table 4 and Fig. 4, is extracted with individual silk gum, common cnidium fruit P.E or mistletoe
Object processing the case where compare, with their mixture handle in the case where cell because of LPS caused by NO synthetic quantity reduce to
About 0.76 to 0.66 times or less.
By the above results it is found that silk gum, common cnidium fruit P.E and Herba Visci extract mixture and they individually make
Substantially reduced with compared to the NO output of macrophage, thus releive to skin regeneration, skin, stimulation is alleviated, wound healing etc.
Show excellent effect.
In addition, confirmation common cnidium fruit P.E or Herba Visci extract all have excellent anti-inflammatory effect at various concentrations
Fruit shows synergistic effect concentration difference.
The application person of ordinary skill in the field is can be regarded as from above explanation in the skill for not changing the application
It can implement the application in the case where art thought or essential feature in other specific ways.Related to thisly, it is thus understood that with
Upper the embodiment described is only exemplary rather than limited in all respects.Scope of the present application should be interpreted that from than upper
State the meaning that aftermentioned claims are described in detail and range and have altered derived from its equivalents or modification after
Mode include within the scope of application.
<110>(strain) natural person
<120>it releives comprising silk gum, the skin regeneration of common cnidium fruit P.E and Herba Visci extract, skin or wound healing is used
Composition
<130> OPA19017-PCT
<150> KR 10-2018-0003989
<151> 2018-01-11
<160> 4
<170> KoPatentIn 3.0
<210> 1
<211> 19
<212> DNA
<213>artificial sequence
<220>
<223>elastin laminin F primer
<400> 1
tcgcaacacg taacgcaat 19
<210> 2
<211> 23
<212> DNA
<213>artificial sequence
<220>
<223>elastin laminin R primer
<400> 2
acttctcttt ttccacccca ttt 23
<210> 3
<211> 19
<212> DNA
<213>artificial sequence
<220>
<223>gapdh F primer
<400> 3
cctcaagatc atcagcaat 19
<210> 4
<211> 20
<212> DNA
<213>artificial sequence
<220>
<223>gapdh R primer
<400> 4
ccatccacag tcttctgggt 20
Claims (10)
1. a kind of skin regeneration, skin are releived or wound healing applies some make up composition includes silk gum, common cnidium fruit P.E and Mongolian oak
Herba Visci extract.
2. cosmetic composition according to claim 1, wherein
The skin regeneration or the wound healing pass through the proliferation of promotion keratinocyte, migration or elastin gene
Expression is to realize.
3. cosmetic composition according to claim 1, wherein
The skin is releived through anti-inflammatory activity and is realized.
4. cosmetic composition according to claim 3, wherein
The anti-inflammatory NO by inhibiting macrophage synthesizes to realize.
5. cosmetic composition according to claim 1, wherein
The composition, which has, selects free solution, externally-applied ointment, frost, foam, nutrition toner, soft toner, facial mask, softness
Water, lotion, sun screen, essence, perfumed soap, cleaning liquid material, balneation agent, suncream, sunblock lotion, suspension, emulsion, paste
Agent, gel, dew, powder, perfumed soap, the facial cleanser containing surfactant, oil, powder foundation, emulsion foundation cream, wax powder bottom, pad pasting and
The dosage form in group formed by spraying.
6. a kind of skin regeneration, skin are releived or wound healing skin preparations for extenal use, posted comprising silk gum, common cnidium fruit P.E and Mongolian oak
Raw extract.
7. a kind of skin regeneration, skin are releived or the outer product composition of wound healing medicine, include silk gum, common cnidium fruit P.E and
Herba Visci extract.
8. a kind of skin regeneration, skin are releived or wound healing health functional food composition, extracted comprising silk gum, frutus cnidii
Object and Herba Visci extract.
9. a kind of composition comprising silk gum, common cnidium fruit P.E and Herba Visci extract is releived for skin regeneration, skin
Or the purposes of wound healing.
10. a kind of composition comprising silk gum, common cnidium fruit P.E and Herba Visci extract be used to prepare for skin regeneration,
Skin is releived or the purposes of the cosmetics of wound healing.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2018-0003989 | 2018-01-11 | ||
| KR1020180003989A KR102033073B1 (en) | 2018-01-11 | 2018-01-11 | Compositions for skin regeneration, skin soothing or wound healing comprising sericin, extracts of erect hedge parsley extract and extracts of mistletoe |
| PCT/KR2019/000454 WO2019139403A1 (en) | 2018-01-11 | 2019-01-11 | Composition containing sericin, torilis japonica extract, and viscum album extract for skin generation, skin soothing, or wound healing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110248641A true CN110248641A (en) | 2019-09-17 |
Family
ID=67219754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201980000271.8A Pending CN110248641A (en) | 2018-01-11 | 2019-01-11 | Skin regeneration, skin comprising silk gum, common cnidium fruit P.E and Herba Visci extract are releived or wound healing composition |
Country Status (3)
| Country | Link |
|---|---|
| KR (1) | KR102033073B1 (en) |
| CN (1) | CN110248641A (en) |
| WO (1) | WO2019139403A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112515980A (en) | 2019-09-18 | 2021-03-19 | 株式会社Lg生活健康 | Cosmetic composition comprising high molecular weight sericin |
| KR102409404B1 (en) | 2019-09-18 | 2022-06-15 | 주식회사 엘지생활건강 | Cosmetic Composition Comprising High Molecular Weight Sericin |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20000056836A (en) * | 1999-02-26 | 2000-09-15 | 정찬복 | The extracting method of sericin and use thereof |
| JP2003252744A (en) * | 2002-02-28 | 2003-09-10 | Seiren Co Ltd | Multifunctional protective agent against ultraviolet hazard |
| KR20170137552A (en) * | 2016-06-03 | 2017-12-13 | 주식회사 엘지생활건강 | Composition for improving skin condition comprising herb extracts mixture |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101303295B1 (en) * | 2011-07-22 | 2013-09-03 | 이상록 | The cosmetic composition for promoting cell growth and recovery of distrupted skin containing the extracts of fermented Mistletoe, fermented Imperata cylindrica and fermented soybean |
-
2018
- 2018-01-11 KR KR1020180003989A patent/KR102033073B1/en active IP Right Grant
-
2019
- 2019-01-11 CN CN201980000271.8A patent/CN110248641A/en active Pending
- 2019-01-11 WO PCT/KR2019/000454 patent/WO2019139403A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20000056836A (en) * | 1999-02-26 | 2000-09-15 | 정찬복 | The extracting method of sericin and use thereof |
| JP2003252744A (en) * | 2002-02-28 | 2003-09-10 | Seiren Co Ltd | Multifunctional protective agent against ultraviolet hazard |
| KR20170137552A (en) * | 2016-06-03 | 2017-12-13 | 주식회사 엘지생활건강 | Composition for improving skin condition comprising herb extracts mixture |
Also Published As
| Publication number | Publication date |
|---|---|
| KR102033073B1 (en) | 2019-10-16 |
| KR20190085748A (en) | 2019-07-19 |
| WO2019139403A1 (en) | 2019-07-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6606085B2 (en) | Method for producing human cells having increased colony generating ability and proliferation ability | |
| TW201206494A (en) | Use of Tiliacora triandra in cosmetics and compositions thereof | |
| KR20040068986A (en) | Drugs for ameliorating itch, rough skin or hypersensitive skin or for whitening via inhibition of the production and release of stem cell factor | |
| CA2780225A1 (en) | Aloe vera sprout concentrate or extract having superior skin cell promotion, antioxidant, and anti-allergy effects | |
| JP2021176855A (en) | Composition for protein l-isoaspartate methyltransferase activation | |
| JP4969849B2 (en) | NF-κB activation inhibitor and external preparation composition II containing the same | |
| CN110248641A (en) | Skin regeneration, skin comprising silk gum, common cnidium fruit P.E and Herba Visci extract are releived or wound healing composition | |
| JP6513468B2 (en) | A skin external preparation or an internal preparation containing an extract of red beet grown by irradiation with light having a specific wavelength range. | |
| JP2015027998A (en) | Moisturizer, skin barrier function activator, tight junction formation accelerator, trpv4 expression enhancer, intracellular calcium concentration increaser, intracellular calcium concentration increaser, lipid synthesis accelerator, blood flow improver and periocular darkness ameliorator | |
| CN106999527A (en) | For preventing hair loss or stimulating the composition for including umbel parthenium extract of natural on-off cycles of hair growth | |
| JP2003171225A (en) | COMPOSITION FOR INTEGRINalpha6beta4 PRODUCTION PROMOTION | |
| JP2019147824A (en) | Kaempferia parviflora-containing compositions | |
| JP2021521264A (en) | Composition for hair loss treatment and hair growth promotion | |
| CN106999528A (en) | For preventing hair loss or stimulating the composition for including high mountain radix scutellariae extract of natural on-off cycles of hair growth | |
| KR20130040664A (en) | A cosmetic composition comprising an extract of rhus verniciflua stokes for trichogenousness | |
| KR102386625B1 (en) | A composition for stimulating growth of hairs contaning peptide complex | |
| CN111447937A (en) | Synergistic composition as autophagy promoting agent | |
| JP2016188180A (en) | Composition containing specific component | |
| JP2010024211A (en) | Cell proliferation promoting agent | |
| JP2007176878A (en) | NF-kappaB ACTIVATION INHIBITOR AND EXTERNAL PREPARATION COMPOSITION I CONTAINING THE SAME | |
| JP2012025777A (en) | Vascularization inhibitor and external preparation | |
| JP2021514965A (en) | Wrinkle improving composition containing skunk cabbage extract or its fraction as an active ingredient | |
| JP2014114251A (en) | Ceramide production promoter | |
| JP5866139B2 (en) | Pleurotus extract used in pharmaceutical and cosmetic compositions | |
| JP6646534B2 (en) | Basic fibroblast growth factor production promoter |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| AD01 | Patent right deemed abandoned |
Effective date of abandoning: 20230825 |
|
| AD01 | Patent right deemed abandoned |