JP2015027998A - Moisturizer, skin barrier function activator, tight junction formation accelerator, trpv4 expression enhancer, intracellular calcium concentration increaser, intracellular calcium concentration increaser, lipid synthesis accelerator, blood flow improver and periocular darkness ameliorator - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a moisturizer, skin barrier function activator, tight junction formation accelerator, TRPV4 expression enhancer, intracellular calcium concentration increaser, lipid synthesis accelerator, blood flow improver and periocular darkness ameliorator having excellent effects.SOLUTION: A black ginger processed product is contained as a moisturizer, skin barrier function activator, tight junction formation accelerator, TRPV4 expression enhancer, intracellular calcium concentration increaser, lipid synthesis accelerator, blood flow improver and periocular darkness ameliorator.

Description

  The present invention includes a moisturizer containing a processed black ginger product, a skin barrier function improving agent, a tight junction formation promoting agent, a TRPV4 expression increasing agent, an intracellular calcium concentration increasing agent, a lipid synthesis promoting agent, a blood flow improving agent and a bear improving agent. It relates to the agent.

Conventionally, it has been known that maintaining and improving the moisture retention of skin is effective for rough skin, dry skin, reduced elasticity, wrinkles, sagging and the like. Skin moisturizing involves various mechanisms, such as retention of moisture by the stratum corneum and suppression of transpiration of moisture by the lipid layer between the stratum corneum cells. The formation of tight junctions and promotion of lipid synthesis promotes skin moisturization. It is known to be effective in maintaining and improving sex.
One of the important roles of the skin is a barrier function that controls the permeation of substances, such as preventing moisture from evaporating excessively from the inside of the body and preventing foreign substances such as bacteria from entering the body. is there. It is known that the barrier function of the skin is destroyed by drying in the winter or exposure to excessive ultraviolet rays.

  Tight junctions exist in the form of a belt around cells and control the permeation of substances by bringing adjacent cells into close contact and sealing. Since the barrier function of the stratum corneum has developed in the skin, it has been considered that tight junctions do not exist. However, it is clear that there are tight junction constituent proteins such as occludin, claudin 1 and claudin 4 in the epidermis / granular layer, and the continuous tight junction consisting of these plays an important role in substance permeability and acts as a permeation barrier. (Non-Patent Document 1). Furthermore, due to the activation of low molecular weight G protein Rac1 and polar protein aPKC, tight junction constituent proteins are lined up on a continuous line (Non-patent Document 2), and the amount of claudin 4 decreases between cells. It has also been reported that substance permeability increases (Non-patent Document 3). In addition, it has been reported that the expression level of a protein that forms a tight junction increases when transient receptor potential channel subfamily V member 4 (TRPV4) is activated (Non-Patent Document 4).

In recent years, attempts have been made to search for materials that enhance the tight junction function and materials that promote the synthesis of intercellular lipids. For example, ganglioside promotes the formation of tight junctions and prevents invasion of allergens (Patent Document 1), gastrointestinal mucosa function maintaining agent containing gastrointestinal hormones as an active ingredient (Patent Document 2), nucleic acids and degradation products thereof Tight junction formation accelerators (Patent Document 3) as active ingredients, peptides and amino acids (Patent Document 4) having allergen absorption inhibitory activity, and the like are disclosed.
Also disclosed are intercellular lipid synthesis promoters (Patent Document 5) containing, as active ingredients, vitamin B3 derivatives such as nicotinic acid and nicotinic acid amide, eucalyptus, silvanal pinus, potato or extracts thereof.
However, it has not yet been satisfactory in terms of stability and economics in the preparation, and development of a material having no side effects has been desired.

  On the other hand, poor blood circulation in the skin is considered to be the cause of skin and skin troubles such as skin color dullness and unevenness, shimoyake, and coldness. As therapeutic agents for these diseases, plant-derived oils with little irritation to the skin, ointments and creams containing moisturizers, etc. are widely studied.

  As blood circulation promoting components used in these therapeutic agents, for example, fats and oils components obtained from shea seeds, plant extracts such as nicotinic acid derivatives, vitamin E derivatives, assembly extracts, etc. are known (for example, patent documents). 6 and 7).

  However, conventional external preparations for improving blood flow have a problem that the effect persistence is poor. Moreover, since the active ingredient of the plant-derived blood flow improving external preparation is not clear, the effect obtained by the same extract is likely to be different, and the use of chemical substances such as vitamin E is limited due to the properties of each substance. There was a problem.

JP-A-8-109133 JP-A-8-268908 Japanese Patent Laid-Open No. 9-30978 JP 2002-257814 A JP 2004-107261 A Japanese Patent Publication No. 7-55888 Japanese Patent Laid-Open No. 3-190809

Experimental Dermatology, 16, p324, 2007 J. et al. Invest. Dermatol. , 127, p782, 2007 J. et al. Cell Biol. , 145, p195, 1999 Skin Pharmacology and Physiology, 26, p15, 2013

  The present invention was made in view of the above circumstances, and has a humectant having an excellent effect, a skin barrier function improving agent, a tight junction formation accelerator, a TRPV4 expression increasing agent, an intracellular calcium concentration increasing agent, a lipid synthesis promoting agent, The object is to provide a blood flow improving agent and a bear improving agent.

  As a result of various studies to solve the above problems, the present inventors have found that the processed black ginger is a moisturizer, a skin barrier function improving agent, a tight junction formation accelerator, a TRPV4 expression increasing agent, an intracellular calcium concentration. It has been found that it has excellent action as a raising agent, lipid synthesis promoter, blood flow improving agent, and bear improving agent, and has completed the present invention.

More specifically, the present invention is as follows. That is,
<1> A humectant containing a processed black ginger product.
<2> A skin barrier function improving agent containing a processed black ginger product.
<3> A tight junction formation accelerator containing a processed black ginger product.
<4> A TRPV4 expression increasing agent containing a processed black ginger product.
<5> An intracellular calcium concentration increasing agent containing a processed black ginger product.
<6> A lipid synthesis promoter containing a processed black ginger product.
<7> A blood flow improving agent containing a processed black ginger product.
<8> A bear improving agent containing a processed black ginger product.

  According to the present invention, the processed black ginger product has excellent moisturizing, skin barrier function improvement, tight junction formation promotion, TRPV4 expression increase, intracellular calcium concentration increase, fat synthesis promotion, blood flow improvement and bear improvement effect. Can do.

It is a figure which shows the gene expression level of TRPV4 by black ginger. It is a figure which shows the increase amount of intracellular calcium concentration by black ginger. It is a figure which shows the relative value of the human body temperature change by black ginger. It is a figure which shows the lipid synthesis amount by black ginger.

  Hereinafter, embodiments of the present invention will be described in detail. However, the present invention is not limited to the following embodiments, and can be implemented with appropriate modifications within the scope of the object of the present invention. .

<Moisturizing agent, skin barrier function improving agent, tight junction formation promoter, TRPV4 expression increasing agent, intracellular calcium concentration increasing agent, lipid synthesis promoter, blood flow improving agent and bear improving agent>
The moisturizing agent, skin barrier function improving agent, tight junction formation promoting agent, TRPV4 expression increasing agent, intracellular calcium concentration increasing agent, lipid synthesis promoting agent, blood flow improving agent and bear improving agent of the present invention are black ginger processed products. It contains, and further contains other components as necessary.

The black ginger in the present invention is a kind of plant belonging to the genus Zingiberaceae Kaempferia, and its scientific name is Kaempferia parviflora. Black ginger is distributed in Southeast Asia and has other names such as black turmeric or Krachaidam. In addition, black ginger is known as a health food in traditional medicine such as Thailand and Laos, and is said to have effects such as enhancement of energy and nutrition. Active ingredients contained in black ginger include selenium and amino acids, as well as, for example, curcumin and polyphenols, which have an antioxidant action to remove active oxygen.
Since black ginger is a natural plant that has been ingested by humans for a long time and has high safety, the water-soluble composition of the present invention has high practicality, as it is or by processing, It can be applied to various uses such as food and drink, cosmetics, pharmaceuticals, and quasi drugs.

  The use site of black ginger in the present invention is not particularly limited as long as it contains a component that contributes to a desired pharmacological action, and examples thereof include roots, leaves, stems, flowers, branches, and the like. A rhizome containing a large amount of polymethoxyflavonoid (PMF) such as dimethoxyflavone (57DMF) is preferred.

The black ginger processed product of the present invention includes, for example, crushed products, pulverized products, shredded products, juices, extracts, fractions of components in black ginger, and these dried products. Here, the extract is not particularly limited as long as the components in black ginger are extracted. For example, an extract obtained by extracting black ginger or a processed product thereof with a solvent, a diluted solution or a concentrated solution thereof. Or a dry product thereof or a powder thereof.
The processed black ginger used in the present invention is preferably an extract such as black ginger extract or squeezed, considering the ease of application of foods and drinks, pharmaceuticals, etc., and the state thereof may be solid or liquid good.

Examples of the crushed and pulverized black ginger used in the present invention include, for example, after washing, drying the sliced black ginger using the sun or a dryer, and then chopping it as it is or into an appropriate shape or size. The processed product thus obtained can be obtained by pulverization using a pulverizer. The order of drying and grinding may be reversed. As the pulverizer, those commonly used can be widely used. For example, a pulverizer constituted by a raw material hopper, a pulverizer, a classifier and a product holder can be used.
Examples of the dried ginger extract or squeezed juice include, for example, the ginger extract or squeezed as it is or concentrated, and further dried. Drying is performed by a method commonly used by those skilled in the art, such as spray drying, freeze drying, reduced pressure drying, and fluidized drying.

The black ginger extract can be obtained by extracting black ginger or its crushed material, pulverized material, juice, etc. with an appropriate solvent. Solvents used for extraction include water, alcohols such as ethanol, methanol, isopropanol, butanol, 1,3-butanediol (butylene glycol), glycerin, lower esters such as ethyl acetate and methyl acetate, and acetone. Examples thereof include organic solvents and mixtures of these organic solvents and water. Among these, since the composition of the present invention is supposed to be ingested by humans, it is preferable to use water alone, ethanol alone or a mixed solvent of water and ethanol (so-called hydrous ethanol).
The concentration of the solvent in the case of using a mixture of an organic solvent and water as the extraction solvent is not particularly limited, but is, for example, 5 to 95 v / v%, preferably 20 to 80 v / v%, more preferably 40 to 70 v / v. v% can be selected.

The extraction method for obtaining the extract of black ginger or the fraction of the components in black ginger is not particularly limited, but it is preferably performed under as mild conditions as possible from the viewpoint of safety and convenience. For example, black ginger or a dried product thereof is pulverized, crushed or shredded, 2 to 20 times the mass of solvent is added thereto, and the mixture is allowed to stand, shake for 10 minutes to 48 hours in the range of 0 ° C. to the reflux temperature of the solvent, Examples include a method in which extraction is performed under arbitrary conditions such as stirring or reflux, and after the extraction operation, insoluble matters are separated by filtration or centrifugation to obtain an extract. The obtained extract may be diluted or concentrated as necessary.
In addition, when the extraction solvent is water, extraction with hot water at a temperature of 95 to 100 ° C., filtration of the extract reaching the maximum concentration, followed by spray drying, or shaking at room temperature (about 25 ° C.) It is also possible to obtain the extract by a method such as extraction and spray drying after filtering the extracted solution reaching the fine tail concentration.
Further, the same operation may be repeated for the insoluble matter (residue) remaining after extraction, and the extract of the insoluble matter (residue) may be used in combination with the previous extract, and this step may be repeated. Further, the extract obtained by these methods may be further purified by a method commonly used by those skilled in the art.

  Examples of components in the black ginger used in the present invention include curcumin, methoxyflavones, and anthocyanidins. Any one of these may be used as a fraction of the components in black ginger, or two or more may be mixed and used.

  Moreover, you may use what was shape | molded into powder, a granular form, granular form, etc. as a black ginger processed material. The molding method is not particularly limited, and when granulation is particularly necessary, a known granulation method such as wet or dry method can be used after adding an appropriate binder or excipient. .

  The particle diameter of the powdered or granulated particles of the black ginger processed product is not particularly limited and can be appropriately selected depending on the purpose.

  The moisturizing agent, skin barrier function improving agent, tight junction formation promoting agent, TRPV4 expression increasing agent, intracellular calcium concentration increasing agent, lipid synthesis promoting agent, blood flow improving agent and bear improving agent of the present invention include black ginger processed products. In addition, various compounds can be blended as long as the object of the present invention can be achieved. For example, another substance having a moisturizing effect, a skin barrier function improving effect, a TRPV4 expression increasing effect, a lipid synthesis promoting effect, a blood flow improving effect and a bear improving effect may be blended.

  The moisturizing agent, skin barrier function improving agent, tight junction formation promoting agent, TRPV4 expression increasing agent, intracellular calcium concentration increasing agent, lipid synthesis promoting agent, blood flow improving agent and bear improving agent of the present invention, As a parenteral composition or an oral composition, it can be used as it is or mixed with other components.

  The moisturizer, skin barrier function improving agent, tight junction formation promoting agent, TRPV4 expression increasing agent, intracellular calcium concentration increasing agent, lipid synthesis promoting agent, blood flow improving agent and bear improving agent of the present invention are parenteral compositions. For example, it can be used as a form suitable for cosmetics. For example, it can be processed into various forms such as lotions, emulsions, gels, creams, ointments and the like. Specifically, it can be used as a lotion, cosmetic cream, milky lotion, cream, pack, hair tonic, hair cream, shampoo, hair rinse, treatment, facial cleanser, foundation, hair restorer, aqueous ointment, spray and the like.

  The moisturizing agent, skin barrier function improving agent, tight junction formation promoting agent, TRPV4 expression increasing agent, intracellular calcium concentration increasing agent, lipid synthesis promoting agent, blood flow improving agent and bear improving agent of the present invention are added to the oral composition. It can be used as a suitable form. For example, the moisturizing agent, skin barrier function improving agent, tight junction formation promoting agent, TRPV4 expression increasing agent, intracellular calcium concentration increasing agent, lipid synthesis promoting agent, blood flow improving agent and bear improving agent of the present invention, Or mixed with excipients such as dextrin, starch, sugar, calcium phosphate, additives, extenders, binders, thickeners, emulsifiers, colorants, fragrances, perfume oils and other additives usually used for processing food and drink Or an oral composition. For example, royal jelly, vitamins, proteins, calcium, chitosan, lecithin and the like may be included to provide a function as nutritional supplement. Moreover, for the purpose of adjusting the taste of the water-soluble composition of the present invention, a sugar solution, a seasoning or the like may be contained. The amount of the additive used is not particularly limited as long as it does not hinder the solution of the problems of the present invention, and is appropriately adjusted.

  The oral composition can take various forms such as liquid, paste, cream, capsule, gel, jelly, gummy, and syrup. Further, the moisturizing agent, skin barrier function improving agent, tight junction formation promoting agent, TRPV4 expression increasing agent, intracellular calcium concentration increasing agent, lipid synthesis promoting agent, blood flow improving agent and bear improving agent of the present invention are used as additives and carriers. Powdered, granular, granular, tablet, rod, plate, block, solid, round, caplet, tablet, wafer, biscuit, cookie, etc. , Chewable, stick, etc. The moisturizing agent, skin barrier function improving agent, tight junction formation promoting agent, TRPV4 expression increasing agent, intracellular calcium concentration increasing agent, lipid synthesis promoting agent, blood flow improving agent and bear improving agent of the present invention depend on their forms. It may be taken orally as it is, or may be taken orally after dissolving in water.

  Black ginger processing contained in the moisturizing agent, skin barrier function improving agent, tight junction formation promoting agent, TRPV4 expression increasing agent, intracellular calcium concentration increasing agent, lipid synthesis promoting agent, blood flow improving agent and bear improving agent of the present invention The amount of the product is not particularly limited, but for oral administration, for example, 0.0001 wt% or more, preferably 0.001 wt% or more, and for parenteral administration such as cosmetics, for example, 0. It can be blended at 00001 vol% or more, preferably 0.0001 vol% or more.

  Next, the present invention will be described in more detail based on examples, but the present invention is not limited thereto.

Example 1
<Measurement of gene expression level of TRPV4>
First, a dried powder of black ginger processed product (hydrous ethanol extract of rhizome) is dissolved in a dimethyl sulfoxide (DMSO) solution to a concentration of 0.4 and 2.0 μg / mL, and a DMSO solution containing black ginger extract is added. Was prepared.
Next, the prepared black ginger extract-containing DMSO solution was diluted with a keratinocyte growth medium (manufactured by Takara Bio Inc.) so that the final DMSO concentration was 0.1%, respectively, to obtain a test substance-containing medium.
Next, normal human epidermal keratinocytes (manufactured by Takara Bio Inc.) are seeded on a 24 well plate so as to be 5.0 × 10 4 cells / well, and 24 using a keratinocyte growth medium (manufactured by Takara Bio Inc.). After pre-culture for an hour, the culture supernatant was removed and replaced with a test substance-containing medium.
After changing to a test substance-containing medium and culturing for 24 hours, the culture supernatant is removed, washed with Dulbecco's phosphate buffered saline (PBS), and RNA is collected from the cells using RNeasy Mini Kit (QIAGEN). did. RNA was synthesized into cDNA using QuantitTect Reverse Transcription kit (manufactured by QIAGEN). The gene expression level of TRPV4 was measured using the obtained cDNA. The results are shown in FIG.

From the results shown in FIG. 1, it was confirmed that the processed black ginger, which is a gene involved in the formation or promotion of tight junction, has an action of increasing the expression of TRPV4.
It is known that when TRPV4 is activated, the expression level of proteins that form tight junctions increases, the formation of tight junctions contributes to the improvement of skin barrier function, and the skin barrier function suppresses transpiration of moisture. From this, it was suggested that the processed black ginger product has an excellent moisturizing effect, skin barrier function improving effect, tight junction formation promoting effect, and TRPV4 expression increasing effect.

(Example 2)
<Measurement of intracellular calcium (Ca ²⁺ ) concentration>
First, a dry powder of processed black ginger (hydrous ethanol extract of rhizome) was dissolved in a dimethyl sulfoxide (DMSO) solution so as to have concentrations of 0.4, 2.0 and 10 μg / mL, and a test substance-containing medium was obtained. Adjusted.
Next, normal human epidermal keratinocytes were seeded on a 24-well plate so as to be 2.0 × 10 4 cells / well, and cultured in a 37 ° C. incubator for 72 hours using a test substance-containing medium.
Next, the supernatant was removed, a DMSO solution having a Fura2-AM concentration of 1 mM / L was added, and the mixture was allowed to stand in an incubator at 28 ° C. for 1 hour. Thereafter, 20 μMRN-1734 (TRPV4 inactivating agent) was added and left in an incubator at 28 ° C. for 30 minutes, and further 10 μM 4α-PDD (TRPV4 activator) was added.
Thereafter, the fluorescence wavelengths F (340) and F (380) at two wavelengths were measured under conditions of excitation wavelengths of 340 nm and 380 nm and a fluorescence wavelength of 540 nm. The fluorescence intensity ratio R (R = F (340) / F (380)) was calculated from the obtained two-wavelength measurement results.

5 μM ionomycin is further added to the sample after the fluorescence intensity measurement, all intracellular calcium probes in the sample are converted into complexes, and the calcium ions are made parallel, and then the fluorescence intensity (F _max (F _max ( 340) and F _max (380)) were measured. From the obtained measurement results, the fluorescence intensity ratio R _max (R _max = F _max (340) / F _max (380)) was calculated.
A 10-m glycol ether diamine tetraacetic acid solution is further added to the sample after the calcium complex is formed, all the calcium complex is removed to make it calcium-free, and the fluorescence intensity (F _min (340)) is obtained under the same conditions as the above measurement conditions. And F _min (380)). From the obtained measurement results, the fluorescence intensity ratio R _min (R _min = F _min (340) / F _min (380)) was calculated.
As a control, the same operation was performed on a DMSO solution containing no black ginger processed product, and the amount of change in calcium concentration was measured.

From these results, the intracellular calcium concentration [Ca ²⁺ ] was calculated using the following formula. At this time, the dissociation constant Kd is the dissociation constant of Fura-2 and is 224 nm / L. The calculation results of the obtained intracellular calcium concentration are shown in Table 1 and FIG.
[Ca ²⁺ ] = Kd × (R−R _min ) / (R _max −R) × F _min (380) / F _max (380)

From Table 1 and FIG. 2, it was confirmed that when the black ginger processed product was blended, the intracellular calcium concentration was higher than when the black ginger processed product was not blended.
Since it is known that the intracellular calcium concentration is increased by the gene expression of TRPV4, it was demonstrated from this result that the processed black ginger product has an effect of increasing the expression of TRPV4.

Example 3
<Confirmation of blood flow improvement effect>
A black ginger processed product similar to that used in Example 1 was dissolved in 1,3-butylene glycol (BG) to give a 50% black ginger suspension. To this suspension, 1000 mL of purified water was added, and a test substance solution was prepared so that the final concentration was 1% black ginger processed product in a 5% BG solution. As a control, 1000 mL of 5% BG aqueous solution was prepared.

  The test was conducted with 6 healthy adult men and women as subjects. In the measurement chamber, the air conditioner was set to 20 ° C. and kept constant. The lights were turned off except when necessary. In addition, the test substance aqueous solution was covered except for the part where the subject put his hand, so that the subject could not be distinguished.

  The test subject washed the forearm portion to be measured, and then entered the measurement room and acclimated for 10 minutes. Next, the body temperature of the forearm to be measured was measured using thermography. Next, the forearm to be measured was dipped in water at about 10 ° C. for 1 minute, and then lightly wiped, and then immersed in an aqueous test substance solution for 1 minute. The body temperature was measured using thermography after immersion in the aqueous test substance solution and 30, 60, 90, and 120 minutes later. The results are shown in Table 2 and FIG. In addition, a graph is a relative value on the basis of the body temperature after immersion in a test substance solution.

From Table 2 and FIG. 3, when the black ginger processed product was blended, the forearm temperature after 30 minutes immersion in the aqueous test substance solution was 1.46 ° C. higher than when the black ginger processed product was not blended. . In addition, when the black ginger processed product was not blended, the temperature dropped after a peak of 60 minutes after the measurement, but was retained even after 120 minutes by blending the black ginger processed product.
From this result, it was confirmed that the blood flow improving effect was obtained by blending the processed black ginger product. That is, it was suggested that ingestion of the processed black ginger product improves blood flow in the lower part of the eye and can eliminate or improve bears.

Example 4
<Confirmation of lipid synthesis promoting effect>
First, a black ginger processed product was dissolved in DMSO so as to have concentrations of 0.4, 2.0, and 10.0 μg / mL, respectively, in the same manner as in Example 1 to prepare a test substance-containing medium.
Next, 0.4% trypan blue solution (manufactured by Sigma Aldrich Co., Ltd.) was used for normal hamster sebaceous gland cells (manufactured by Kurashiki Textile Co., Ltd.) grown in a culture medium for sebaceous gland cells (manufactured by Kurashiki Co., Ltd.). Cell number was measured. From the measurement results, the normal hamster sebaceous gland cells were suspended using a sebaceous gland cell differentiation-inducing medium (manufactured by Kurashiki Boseki Co., Ltd.) such that the number of cells of normal hamster sebaceous gland cells was 4.5 × 10 ⁴ cells / 500 μL, and seeded on a 24-well plate. The cells were cultured for 24 hours in a CO ₂ incubator.
After culturing, the culture supernatant of 24 well plate was removed, replaced with the adjusted test substance-containing medium, and cultured in a CO ₂ incubator. The culture was carried out for 12 days while changing the test substance-containing medium every 2-3 days.
After the culture, the culture supernatant is removed from the 24 well plate, 500 μL of a cell number measurement solution (WST-8 solution) diluted 25-fold with a medium for induction of sebaceous gland cell differentiation is added, and left to stand for 30 minutes in an incubator. Was collected in a 96-well plate, and the cell number was measured from the absorbance (450 nm).
Next, the supernatant remaining on the 24 well plate is removed, washed and stained using PBS (−) buffer, 10% formalin solution, 60% isopropanol solution, oil red O solution, and then 100% isopropanol solution is added. 300 μL was added and shaken (450 rpm, 5 min). After shaking, the supernatant was collected in a 96-well plate, and the amount of lipid was measured from the absorbance (530 nm). The cell number measurement solution, PBS (−) buffer, 10% formalin solution, 60% isopropanol solution, oil red O solution, and 100% isopropanol solution were included in the lipid synthesis measurement kit (manufactured by Kurashiki Boseki Co., Ltd.). I used something.
The amount of lipid synthesis per cell was calculated from the results of the number of cells measured and the amount of lipid. As a control, the same test was performed on a sample to which no test solution was added, and the number of cells and the amount of lipid were calculated. The results are shown in FIG.

From FIG. 4, when the black ginger processed product was blended, the amount of lipid synthesis increased compared to the case where the black ginger processed product was not blended. In addition, when the black ginger processed product was not blended, no change was observed in the amount of lipid synthesis, but by adding the black ginger processed product, an increase in the amount of lipid synthesis was confirmed. From this result, it was confirmed that the processed black ginger has a lipid synthesis promoting action.
Moreover, if the amount of keratin intercellular lipid synthesis increases, the moisture transpiration of the skin can be suppressed, suggesting that the processed black ginger has a moisturizing action.

Below, the formulation example of the product using the black ginger processed material used by this invention is shown.

<Cream>
A cream containing a dry powder of water-containing ethanol (5 v / v%) extract as a processed black ginger product was prepared according to the formulation shown in Table 3. That is, the components (1) to (4) were mixed and heated to 80 ° C. (A). On the other hand, the components (5) to (18) were mixed and heated to 80 ° C. (B). The mixture of A was gradually added to the mixture of A with stirring to emulsify, and then cooled to 35 ° C. to obtain the desired cream.

<Lotion>
As a black ginger processed product, a lotion containing a dry powder of 1,3-butanediol extract was prepared according to the formulation shown in Table 4. That is, the components (1) to (6) were mixed, heated to 80 ° C. to make the mixture uniform, and then cooled. The components (7) to (12) were sequentially added thereto at 35 ° C., mixed and homogenized to obtain the intended lotion.

<Serum>
As a black ginger processed product, a cosmetic solution containing a dry powder of hot water extract was prepared according to the formulation shown in Table 5. That is, (1) to (10) were uniformly mixed and dissolved at 70 ° C. (A). On the other hand, (11) to (20) were uniformly mixed and dissolved at 70 ° C. (B). The mixture was emulsified with a homomixer while A was gradually added to B maintained at 70 ° C. When the emulsification was completed, the product was rapidly cooled to 40 ° C. or lower to obtain the desired cosmetic liquid.

<Emulsion>
As a processed black ginger product, an emulsion containing a dry powder of 1,3-butanediol extract was prepared according to the formulation shown in Table 6. That is, the components (1) to (7) were mixed (A) and heated to 80 ° C. On the other hand, each component of (8)-(13) was heated at 80 degreeC (B). The mixture of B was added to the mixture of A and stirred to emulsify, and (14) and (15) were further added to neutralize, and then the mixture was stirred and cooled to 35 ° C. to obtain the desired emulsion.

<Packing agent>
As a black ginger processed product, a pack containing dry powder of glycerin extract was prepared according to the formulation shown in Table 7. That is, (1) to (3) were mixed and uniformly dissolved at 70 ° C. (A). On the other hand, (4) to (8) were mixed (B). B was added to A to obtain the desired pack agent.

<Hair shampoo>
A shampoo containing a dry powder of water-containing ethanol (40 v / v%) extract as a black ginger processed product was prepared according to the formulation shown in Table 8. That is, (1) to (9) were mixed and dissolved uniformly to obtain the desired shampoo.

<Tablet 1>
Using the dried powder of the pulverized product as a processed black ginger product, tablets that were tableted according to a conventional method with the formulation shown in Table 9 were produced.

<Tablet 2>
Using dry powder of water-containing ethanol (95 v / v%) extract as a processed black ginger product, tablets tableted according to a conventional method with the formulation shown in Table 10 were produced.

<Soft capsule>
A hot water extract was used as a processed black ginger, and the internal solution blended at the blending ratio shown in Table 11 was prepared to produce soft capsules.

<Hard capsule>
As the processed black ginger product, a dry powder of squeezed juice was used to prepare a composition having the formulation shown in Table 12, and a hard capsule was produced by filling the capsule film.

<Granule>
Using dry powder of hydrous ethanol (70v / v%) extract as black ginger processed product, in accordance with the prescription in Table 13, each component is put into a fluidized bed granulator and granulated by spraying water. went. The obtained granulated product was sieved with a 30 mesh sieve to produce granules.

<Liquid drink>
An extract was used as a processed black ginger product, and each component was blended at a blending ratio shown in Table 14 to produce a liquid beverage.

  The processed black ginger used in the present invention has an excellent moisturizing action, skin barrier function improving action, TRPV4 expression increasing action, lipid synthesis promoting action, blood flow improving action and bear improving action. Therefore, it can be preferably used for products such as lotion, milky lotion, cosmetic liquid, cream, pack, shampoo, conditioner, tablet, soft capsule, hard capsule, granule, liquid beverage.

Claims (8)

  A moisturizer containing black ginger processed product.   Skin barrier function improver containing black ginger processed product.   Tight junction formation accelerator containing black ginger processed product.   A TRPV4 expression increasing agent containing a black ginger processed product.   An intracellular calcium concentration increasing agent containing a processed black ginger product.   Lipid synthesis promoter containing black ginger processed product.   Blood flow improving agent containing black ginger processed product.   Bear improver containing black ginger processed product.