JP2003212748A - Collagen production promoter, skin cosmetic and beauty food and drink - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a collagen production promoter containing, as the active ingredient, a natural product found out as a material having collagen production promoting effect, and to provide skin cosmetics and beauty foods and drinks comprising the promoter. <P>SOLUTION: The extract of one or more plant selected from a group consisting of Senecio scandens, Euphorbia chamaesyce (or Euphorbia prostrata), Polygalafallax, Rhodobryum roseum and Alternanthera ressilis is compounded into a collagen production promoter as the active ingredient and the promoter is compounded to skin cosmetics and beauty foods and drinks. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention provides a collagen production promoter having an action of activating the production of collagen by fibroblasts, and a skin cosmetic and a cosmetic action having an action of preventing and improving skin aging. The present invention relates to food and drink (food and drink for beauty use).

[0002]

2. Description of the Related Art The dermis and epidermis of skin are composed of epidermal cells, fibroblasts and extracellular matrix such as collagen that is outside these cells and supports the skin structure. In young skin, the interaction of these skin tissues maintains homeostasis to ensure water retention, flexibility, elasticity, etc., and the skin is kept moisturized and glossy in appearance. .

However, when there is an influence of some external factors such as ultraviolet rays, remarkable drying of air, excessive skin washing, etc., and aging progresses, collagen, which is a major constituent of the extracellular matrix, is produced in a small amount. As well as decreasing, the elasticity decreases due to crosslinking. The influence of external factors and a decrease in the proliferation rate of fibroblasts with aging also cause a decrease in collagen production. As a result, the skin loses its moisturizing function and elasticity,
The keratin begins to exfoliate abnormally, and the skin loses its tension and luster, and becomes aging, such as roughness, wrinkles, and dullness.

As described above, the changes associated with aging of the skin, that is, wrinkles, dullness, loss of texture, decrease in elasticity, etc.
Reduction / denaturation of dermal matrix components such as collagen,
It is associated with a decrease in the proliferation rate of fibroblasts.

In recent years, it has been pointed out that the matrix protease is particularly involved as a factor inducing the above changes. Among matrix-type proteases, collagenase, that is, MMP-1 (matrix metalloprotease) is known as an enzyme that decomposes type I and III collagens, which are the main constituents of the dermal matrix of the skin, and its expression is exposed to ultraviolet rays. It is considered that it may be a major factor such as the formation of wrinkles on the skin, as well as the decrease and degeneration of collagen due to ultraviolet rays.

[0006]

Therefore, the first object of the present invention is to find a substance which can promote the production of collagen by fibroblasts and prevent and / or improve the aging of the skin. To provide a collagen production promoter contained as.

A second object of the present invention is to provide a skin cosmetic which has a collagen production promoting action and is useful for preventing and / or improving skin aging.

A third object of the present invention is to provide a cosmetic food or drink which has a collagen production promoting action and is useful for preventing and / or improving skin aging.

[0009]

In order to solve the above-mentioned object, the collagen production promoter of the present invention is selected from the group consisting of Senrikou, Michijikusa, yellow flower inversion lotus, shinshinso and lotus grass. It is characterized by containing as an active ingredient an extract from one species or two or more species of plants, and the skin cosmetics and beauty foods and beverages of the present invention are Senrikou, aerial ground grass, yellow flower infested lotus, and reed heart plant. And an extract from one or more plants selected from the group consisting of lotus roots.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below. In the present invention, the “extract” is an extract obtained by using one or more plants selected from the group consisting of Senrikou, Michijikusa, Huanghua Inverted Lotus, Huaxia japonicum, and lotus root as an extraction raw material. A liquid, a dilute solution or a concentrated solution of the extract, a dried product obtained by drying the extract, or a crudely purified product or a purified product thereof is included.

The plant to be used as the extraction raw material is one or more plants selected from the group consisting of Senrikou, Machijizo, Huanghua Inverted Water Lotus, Zhisinso and Lotus plant. When two or more kinds of plants are used as extraction raw materials, the above plants can be arbitrarily combined.

Senrikou (Scientific name: Senecio sc
andens) (also known as daisies) is a perennial creeping herb belonging to the family Asteraceae, distributed in Central South, Southwest, East China, etc., and is easily available from these areas. In addition, it is a field grass (scientific name: Euphorbia chamaesyc).
e, Euphorbia prostrata) (also known as dairy grass) is a first-year herb belonging to the Euphorbiaceae family, distributed in Guangdong, Guangxi, Fujian, Sichuan, Yunnan, Guizhou, etc. It is easily available from the area. In addition, the yellow-flowered water lotus (Science name: Polygalafallax) is a shrub that belongs to the family Hemelidae, and is distributed in Central and Southeast China, East China, and Sichuan, and is easily available from these areas. In addition, converted herbs (scientific name: Rhodobryum roseum) (also known as moss, warm-sea foliage moss)
Is a perennial moss plant belonging to the family Scorpionidae, which is widely distributed south of the Yangtze River and is easily available from these areas. Also, lotus root (scientific name: Alte
rnanthera sessilis) (also known as Periwinkle sessilis) is a first-year herb belonging to the family Amaranthaceae.
It is distributed in Central China, South China, Southwest, etc. and is easily available from these areas.

The constituent parts of the plant used as the extraction raw material are not particularly limited. For Senrikou, for example, the constituent parts of whole grass, leaves, stems, flowers, seeds, roots, etc. Is, for example, leaves, stems, flowers, seeds, roots, and other constituent parts, for yellow flower water lily, for example, leaves, branches, flowers, seeds, roots, and other constituent parts, for centrifuges For example, constituent parts such as whole grass can be used as an extraction raw material for lotus grass, for example, constituent parts such as whole grass, leaves, stems, flowers, seeds and roots. Among these constituents, the whole plant is especially for Senrikou, the leaf part is especially for the barren grass, the branch part is especially for the yellow-flowered water lotus, the whole plant is especially for the conversion plant, and the lotus plant is It is particularly preferable to use the leaf portion as an extraction raw material.

The details of the substance having a collagen production promoting action contained in the above-mentioned plant extract are unknown,
By the extraction method that is commonly used to extract plants,
An extract having a collagen production promoting action can be obtained from the above plant. For example, it can be obtained by drying the extraction raw material and then pulverizing it as it is or by using a coarse crusher and subjecting it to extraction with an extraction solvent. At this time, the extraction raw material may be dried in the sun or using a commonly used dryer. Further, the above plants may be subjected to pretreatment such as defatting with a non-polar solvent such as hexane or benzene and then used as an extraction raw material. By performing the pretreatment such as degreasing, the extraction treatment with the polar solvent can be efficiently performed.

As the extraction solvent, it is preferable to use water, a hydrophilic organic solvent or a mixture thereof at room temperature or at a temperature lower than the boiling point of the solvent.

The water that can be used as the extraction solvent includes pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those that have been subjected to various treatments.
Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering and the like.
Therefore, the water that can be used as the extraction solvent in the present invention also includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline and the like.

Examples of the hydrophilic organic solvent which can be used as the extraction solvent include, for example, methanol, ethanol, propyl alcohol, isopropyl alcohol and the like having 1 to 5 carbon atoms.
And lower aliphatic ketones such as acetone and methyl ethyl ketone; and polyhydric alcohols having 2 to 5 carbon atoms such as 1,3-butylene glycol, propylene glycol and glycerin.

When a mixed solution of two or more polar solvents is used as the extraction solvent, the mixing ratio can be adjusted appropriately. For example, when a mixed liquid of water and lower aliphatic alcohol is used, the mixing ratio of water and lower aliphatic alcohol can be 7: 3 to 2: 8 (weight ratio).

The extraction treatment is not particularly limited as long as the soluble component contained in the plant can be eluted in the extraction solvent, and can be carried out by a conventional method. It is not necessary to employ a special extraction method during the extraction treatment, and any device can be used at room temperature or under reflux heating.

Specifically, the extraction raw material is put into a treatment tank filled with an extraction solvent, and the mixture is usually left to stand for 1 to 3 hours while eluting the soluble components with occasional stirring, if necessary, and then filtered. The solid matter is removed, the extraction solvent is distilled off from the obtained extract, and the extract is obtained by drying. The extraction solvent amount is usually 5 to 15 times the extraction raw material amount (weight ratio), and the extraction temperature is usually room temperature to 95 ° C.

The obtained extract is diluted, concentrated, dried according to a conventional method to obtain a diluted solution or concentrated solution of the extracted solution, a dried product of the extracted solution, or a crudely purified product or a purified product thereof. You may perform processing, such as refinement | purification.

The obtained extract can be used as it is as a collagen production promoter, but a concentrate or a dried product is easier to use. The above-mentioned plant extract can be formulated according to a conventional method. In the case of formulation, in order to facilitate storage and handling, dextrin, a pharmaceutically acceptable carrier such as cyclodextrin and any other auxiliaries can be added, and the extract from the plant is powdered, It can be formulated into any dosage form such as a fruit shape and a tablet shape.

Since the extract from the above plant has a peculiar odor, it is possible to carry out purification for the purpose of decolorization, deodorization, etc. within a range that does not impair its physiological activity. Since it is not used in a large amount when added to foods, foods and drinks, there is no practical problem even if it is unrefined. Specifically, the purification can be performed by activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like.

The collagen production promoter of the present invention can prevent and / or improve skin aging by activating the production of collagen by fibroblasts and supplying sufficient collagen to the dermis layer.

[Skin cosmetics] Extracts from the above plants are
Since it has an effect of preventing and / or improving skin aging, and has excellent usability and safety when applied to the skin, it is suitable for incorporation into skin cosmetics. You may mix | blend the collagen production promoter of this invention with skin cosmetics.

The kind of skin cosmetics to which the extract from the above plant can be added is not particularly limited, and specific examples thereof include ointments, creams, emulsions, lotions, packs, lipsticks and bath salts.

The amount of the extract from the above plant in the skin cosmetic of the present invention can be appropriately adjusted depending on the kind of the skin cosmetic and the physiological activity of the extract. It is preferable that the content of the extract is about 0.0001 to 10% by weight in terms of the standard extract.

The skin cosmetic of the present invention contains various main agents and auxiliaries commonly used in the production of skin cosmetics, and other optional auxiliaries, as long as they do not interfere with the collagen production promoting action of the above-mentioned plant extract. Can be used. The skin cosmetic of the present invention is not limited to the above-mentioned extract from the plant as the main ingredient, for prevention and improvement of skin aging.

In the skin cosmetic of the present invention, examples of the skin cosmetic composition which can be used together with the above-mentioned plant extract include astringents, bactericides / antibacterial agents, whitening agents, ultraviolet absorbers and moisturizers. , Cell activating agents, anti-inflammatory / anti-allergic agents, antioxidants / active oxygen scavengers, etc., and when the above constituents are used in combination with the extract from the plant, the constituents used in combination with the extract from the plant The synergistic action between and may result in superior use effects that are normally expected.

In the case of producing a skin cosmetic containing an extract from the above-mentioned plant, there is almost no restriction on the selection of other raw materials, and oils and fats, waxes, hydrocarbons, fatty acids and alcohols. Any of general base materials and auxiliaries such as, esters, surfactants, and fragrances can be used.

[Beverage for Beauty] It has been confirmed that the extract from the above-mentioned plant has an action of preventing and / or improving skin aging, and is not something which is digested in the digestive tract. It is suitable for incorporation into any food or drink or dietary supplement. For food and drink and dietary supplements,
You may mix | blend the collagen production promoter of this invention.

The food and drink containing the extract from the above-mentioned plant has an effect of preventing and / or improving skin aging and can be used as a cosmetic food and drink.
Here, the "food and drink for beauty" means a food and drink for the purpose of preventing and / or improving beautiful skin or aging of the skin.

The cosmetic food or drink of the present invention may be one in which the extract from the plant is mixed with any food or drink that does not interfere with its activity, or the extract from the plant is a main component. May be a dietary supplement.

When producing the cosmetic food or drink of the present invention,
For example, sugars such as dextrin and starch; gelatin,
Proteins such as soybean protein and corn protein;
Amino acids such as alanine, glutamine and isoleucine;
Polysaccharides such as cellulose and gum arabic; soybean oil, fats and oils such as medium-chain fatty acid triglyceride and the like can be added to prepare any dosage form.

The amount of the extract extracted from the above-mentioned plant in the cosmetic food or drink of the present invention is such that the total intake of the extract per day for an adult is about 1 in consideration of the general intake of the food or drink to be added.
It is suitable to make it about 1000 mg.

There are no particular restrictions on the type of food or drink that can contain the extract from the above-mentioned plant, but specific examples thereof include drinks such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks and lactic acid drinks (these drinks). Concentrated stock solution and powder for adjustment); frozen desserts such as ice cream, ice sorbet, shaved ice; noodles such as buckwheat, udon, harusame, gyoza rind, maimai rind, Chinese noodles, instant noodles; candy, chewing gum, candy , Sweets such as gum, chocolate, tablets, snacks, biscuits, jellies, jams, creams, baked goods; processed fish and livestock products such as kamaboko, ham, sausages; dairy products such as processed milk and fermented milk; salad oil, Oils and fats and processed foods such as tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing; sauces, Seasonings such as is; soups, stews, salads, prepared foods, pickles, and the like.

The above-described collagen production promoter, skin cosmetics and cosmetic foods and drinks of the present invention are preferably applied to humans. However, as long as each action and effect is exhibited, it is not human. It can also be applied to other animals.

[0038]

EXAMPLES The present invention will be specifically described below with reference to production examples, test examples and formulation examples, but the present invention is not limited to the following examples.

[Production Example 1] 300 g of the coarsely crushed whole plant of Senrikou, the leaves of the ground, the branches of the yellow-flowered water lotus, the whole plant of the reed plant, and the leaves of the lotus root extract were extracted with 3000 mL of the extraction solvent. And maintained at 80 ° C. for 2 hours with gentle stirring. Water, 50
After performing the above-mentioned extraction treatment using three kinds of extraction solvents of% ethanol or ethanol, the mixture was filtered, the filtrate was concentrated under reduced pressure at 40 ° C, and further dried by a reduced pressure dryer. Table 1 shows each extraction rate when water, 50% ethanol, and ethanol were used as the extraction solvent. When the extraction solvent is a mixture, the mixing ratio is based on weight.

[0040] [Table 1] Extraction rate (wt%) Extraction raw material of the whole plant 19.4 16.8 12.4 Marts land grass of water 50% ethanol ethanol Chisato light leaves 26.7 26.4 7 .5 Branches of yellow-flowered water lotus 25.4 18.0 12.1 Whole plant of 1st time plant 18.4 21.8 7.6 Leaf part of lotus root 28.9 17.2 13.8

[Test Example 1] Collagen production accelerating action test Whole grass of Senrikou, leaf part of ground grass, branch of yellow-flowered water lotus, whole grass of spinach and lotus root plant obtained in Production Example 1 50% of leaves
About the ethanol extract (hereinafter referred to as "sample"),
The collagen production promoting action was tested by the following test method.

Human fibroblasts were seeded on a 96-well plate, and sample-added medium (sample concentration: 100 ppm or 50 p at 37 ° C., 5% CO ₂ -95% air).
pm) (ppm = μg / mL) for several days, 90 μL of the supernatant was transferred to an ELISA plate and allowed to adsorb on the plate at 4 ° C. overnight, then the solution was discarded and 0.05%
Phosphate physiological buffer solution containing Tween-20 (PBS-
In T), washing was performed. After that, a blocking operation was performed with a physiological phosphate buffer containing 1% bovine serum albumin. The solution is discarded and washed with phosphate physiological buffer (PBS-T) containing 0.05% Tween-20,
An anti-human collagen type I antibody (rabbit IgG; manufactured by Chemicon) was reacted. Discard the solution, 0.05% Tw
After washing with a phosphate physiological buffer solution (PBS-T) containing een-20 and reacting with an HRP-labeled anti-rabbit IgG antibody, the same washing operation was performed and a color reaction was performed.

The collagen production accelerating rate was calculated by performing the above-mentioned ELISA using a standard product to prepare a calibration curve, and setting the collagen production amount when the sample was not added as 100%. Table 2 shows the collagen production promotion rate (%) of each sample.

[Table 2] Collagen production promotion rate (%) Extraction raw material 100 ppm 50 ppm Senrikou whole plant 508.5 306.1 Leaf of grassland 154.3 166.7 Branch of yellow flower injured lotus 187.4 134.5 Whole plant of reed heart 165.3 113.8 Leaf part of lotus root 221.0 152.9

From the results shown in Table 2, the whole grass of Senrikou,
50% ethanol extract of the leaves of alluvial grass, the branch of yellow-flowered water lotus, the whole plant of converted herb and the leaves of lotus root grass all have a collagen production promoting action, 50 of the whole plant
It was confirmed that the% ethanol extract has a large collagen production promoting action.

[Formulation Example 1] An emulsion having the following composition was produced by a conventional method. Senrikou 50% Ethanol extract 0.1 g Paponica herb water extract 0.1 g Yellow flower inversion Lotus lotus ethanol extract 0.1 g Spinach plant water extract 0.1 g Lotus root plant ethanol extract 0.1 g Jojoba oil 4 g Olive Oil 2g Squalane 2g Cetanol 2g Glyceryl monostearate 2g Polyoxyethylene cetyl ether (20E.0) 2.5g Polyoxyethylene sorbitan oleate (20E.0) 2g 1,3-butylene glycol 3g Methyl paraoxybenzoate 0.15g Magnesium ascorbyl phosphate 0.1 g Yellow mucous extract 0.1 g Ginkgo biloba extract 0.1 g Conchiolin 0.1 g Oat extract 0.1 g Chamomile extract 0.1 g Dipotassium glycyrrhizinate 0.1 g Perfume 0.05 g Purified water Remainder (total amount 100g)

[Formulation Example 2] A lotion having the following composition was produced by a conventional method. Senriko Water extract 0.1 g Mushroom grass ethanol extract 0.1 g Yellow flower inversion lotus 50% ethanol extract 0.1 g Spinach plant 50% ethanol extract 0.1 g Lotus flower extract 0.1 g Glycerin 3 g 1,3-butylene glycol 3 g Polyoxyethylene sorbitan oleate (20E.0) 0.5 g Methyl paraoxybenzoate 0.15 g Citric acid 0.1 g Sodium citrate 0.1 g Oil-soluble licorice extract 0.1 g Seaweed extract 0. 1 g Xylobiose mixture 0.5 g Kujin extract 0.1 g Perfume 0.05 g Purified water balance (total amount is 100 g)

[Formulation Example 3] A cream having the following composition was produced by a conventional method. Senriko Ethanol Extract 0.1 g Beef Grass 50% Ethanol Extract 0.1 g Yellow Flower Inverted Lotus Water Extract 0.1 g Hydrangea Ethanol Extract 0.1 g Lotus Root 50% Ethanol Extract 0.1 g Liquid Paraffin 5 g salix beeswax 4 g cetanol 3 g squalane 10 g lanolin 2 g stearic acid 1 g polyoxyethylene sorbitan oleate (20E.0) 1.5 g glyceryl monostearate 3 g 1,3-butylene glycol 6 g methyl paraoxybenzoate 1.5 g yeast extract 0 .1 g Perilla extract 0.1 g Linden extract 0.1 g Chinese cabbage extract 0.1 g Perfume 0.1 g Purified water balance (total amount is 100 g)

[Formulation Example 4] A pack having the following composition was produced by a conventional method. Senrikou Ethanol Extract 0.1 g Mushroom Grass Ethanol Extract 0.1 g Yellow Flower Inverted Lotus Lotus Water Extract 0.1 g Hydrangea 50% Ethanol Extract 0.1 g Lotus Root Water Extract 0.1 g Polyvinyl Alcohol 15 g Polyethylene Glycol 3 g Propylene glycol 7 g Ethanol 10 g Ethyl paraoxybenzoate 0.05 g Tocopherol acetate 0.1 g Sage extract 0.1 g Toki extract 0.1 g Carrot extract 0.1 g Perfume 0.05 g Purified water balance (total amount to 100 g) )

[Formulation Example 5] The following mixture was tabletted to produce a tablet nutritional supplement. Senri Kosui Water Extract 1 part by weight Mushokushiso Water Extract 1 part by weight Yellow Flower Inverted Lotus Water Extract 1 part by weight Converted Plant Water Extract 1 part by weight Lotus Flower Extract 1 part by weight Powdered sugar (sucrose) 188 parts by weight Glycerin fatty acid ester 12 parts by weight

[Formulation Example 6] The following mixture was formed into granules to prepare a dietary supplement. Senrikou 50% ethanol extract 1 part by weight Mushroom grass 50% ethanol extract 1 part by weight Yellow flower inversion lotus 50% ethanol extract 1 part by weight Spinach 50% ethanol extract 1 part by weight Lotus flower 50% ethanol extract 1 part by weight Beet oligosaccharides 1000 parts by weight Vitamin C 167 parts by weight Stevia extract 10 parts by weight

[0052]

INDUSTRIAL APPLICABILITY The present invention provides a collagen production promoter. The collagen production promoter of the present invention is useful for preventing and / or improving skin aging. Also,
The present invention provides a skin cosmetic having a collagen production promoting action. The skin cosmetic of the present invention has excellent usability and safety when applied to the skin,
It is useful for preventing and / or improving skin aging.
Further, according to the present invention, there is provided a cosmetic food or drink product having an action of promoting collagen production. The cosmetic food and drink of the present invention is useful for preventing and / or improving skin aging.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 35/78 A61K 35/78 CLT A61P 17/16 A61P 17/16 43/00 107 43/00 107 A23L 2/00 F (72) Inventor Masazumi Miyakoshi 1089-8 Aikata, Shinichi-machi, Ashina-gun, Hiroshima Prefecture Maruzen Pharmaceutical Co., Ltd. Research Institute (72) Zenhito Kawashima 1089-8, Aikata-machi, Ashina-gun, Hiroshima Prefecture Maruzen Pharmaceutical F-Term (Reference) in Research Institute Co., Ltd. AC04 AC05 AC11 CA04 CA05 NA14 ZA89 ZB21

Claims (3)

[Claims] 1. An extract as an active ingredient from one or more plants selected from the group consisting of Senrikou, aerial ground grass, yellow flower inversion lotus, revolving grass and lotus root grass. Collagen production promoter. 2. A skin characterized by being blended with an extract from one or more plants selected from the group consisting of Senrikou, Michijikusa, yellow-flowered green lotus, revolving grass and lotus root grass. Cosmetics. 3. A beauty comprising an extract from one or two or more plants selected from the group consisting of Senrikou, Michijikusa, yellow-flowered green lotus, revolving grass and lotus root grass. Food and drink.