JP2003212781A - Hyaluronic acid production promoter, skin cosmetic and food and beverage for beautification - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a hyaluronic acid production promoter which is prepared by finding a substance having promoting action on hyaluronic acid production from natural products and comprises the substance as an active ingredient, to provide a skin care preparation and a food and beverage mixed with the promoter. <P>SOLUTION: This hyaluronic acid production promoter comprises extracts from one or more kinds of plants selected from the group consisting of Salononia cantoniensis, Euphorbia chamaesyce and Sterculia lychnophera as an active ingredient. The skin care preparation and the food and beverage for beautification are mixed with the hyaluronic acid production promoter. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a hyaluronic acid production promoter having an action of activating the production of hyaluronic acid by fibroblasts, as well as a skin cosmetic and a cosmetic action having a skin aging preventing / ameliorating action. The present invention relates to the given food and drink (food and drink for beauty).

[0002]

2. Description of the Related Art The dermis and epidermis of skin are composed of epidermal cells, fibroblasts and extracellular matrix such as hyaluronic acid, which is outside these cells and supports the skin structure. In young skin, the interaction of these skin tissues maintains homeostasis to ensure water retention, flexibility, elasticity, etc., and the skin is kept moisturized and glossy in appearance. .

However, due to the influence of certain external factors such as ultraviolet rays, drastic drying of air, excessive skin washing, etc. and the progress of aging, the influence of external factors and the fibers associated with aging A decrease in the growth rate of blast cells causes a decrease in the production of hyaluronic acid, which is a natural moisturizing factor.
As a result, the skin loses its moisturizing function and elasticity, and the keratin begins to exfoliate abnormally, causing the skin to lose its tension and luster, and to exhibit aging symptoms such as roughness, wrinkles, and dullness.

As described above, the changes associated with aging of the skin, that is, wrinkles, dullness, loss of texture, decrease in elasticity, etc.
It is involved in the decrease / denaturation of dermal matrix components such as hyaluronic acid and the decrease in the proliferation rate of fibroblasts.

[0005]

Therefore, the first object of the present invention is to find a substance capable of promoting the production of hyaluronic acid by fibroblasts to prevent and / or improve the aging of the skin. It is to provide a hyaluronic acid production promoter contained as a component.

A second object of the present invention is to provide a skin cosmetic having a hyaluronic acid production promoting action and useful for preventing and / or improving skin aging.

A third object of the present invention is to provide a cosmetic food or drink which has a hyaluronic acid production promoting action and is useful for preventing and / or improving skin aging.

[0008]

In order to solve the above-mentioned object, the hyaluronic acid production promoter of the present invention comprises one or more plants selected from the group consisting of Fume cloud, Mushroom grass and Tsutsumi sea. Is contained as an active ingredient, and the skin cosmetic and the food and drink for cosmetics of the present invention are one or more plants selected from the group consisting of Fukimokusa, Michijikusa and Tsutsumi sea. It is characterized by containing the extract from

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below. In the present invention, the "extract" is an extract obtained by using one or two or more plants selected from the group consisting of fukuromogusa, abandoned grass and Tsutsumi sea as an extraction raw material, a diluted solution of the extract or A concentrated solution, a dried product obtained by drying the extract, or a crudely purified product or a purified product thereof is included.

The plant used as the extraction raw material is one or two or more plants selected from the group consisting of cloud-bloom, grassland, and open sea. When two or more kinds of plants are used as extraction raw materials, the above plants can be arbitrarily combined.

Fukimokusa (scientific name: Salononi)
a cantoniensis) (also known as salamou, Ichiwanho), Koujisou, overland snake (Kasanda),
Zanjaken is a first-year herb belonging to the family Hemelidae, distributed in Guangdong, Guangxi, Yunnan, Guizhou, Hunan, Jiangxi, Fujian, etc., and is easily available from these areas. In addition, it is a field grass (scientific name: Euph)
orbia chamaesyce, Euphorbia prostrata (also known as milkweed grass) is an annual herb belonging to the Euphorbiaceae family, Cantonese, Guangxi,
It is distributed in Fujian, Sichuan, Yunnan, Guizhou, etc. and is easily available from these areas. In addition, Tsukaikai (scientific name: Sterculia lychnophera) is a deciduous tree that belongs to the family Aequiliidae and is distributed in tropical regions such as Vietnam, India, Malaysia, Thailand, and Indonesia. It is available.

The constituent parts of the plant used as the extraction raw material are not particularly limited, and as for the Fukurokumo, for example, the constituent parts of whole grass, leaves, stems, flowers, seeds, roots, etc. Is, for example, whole grass, leaves, stems, flowers, seeds,
Constituent parts such as the root, for the open sea, for example, the leaves,
Constituent parts such as branches, bark, flowers, seeds and roots can be used as the extraction raw material. Of these constituent parts, it is preferable to use leaf and / or branch as the extraction material, especially for the Fukurokumo, the leaf for the ground grass, and the seed for the open sea.

The details of the substance having a hyaluronic acid production-promoting effect contained in the extract from the plant are unknown, but the hyaluronic acid production-promoting action from the plant is carried out by an extraction method generally used for plant extraction. It is possible to obtain an extract having For example, it can be obtained by drying the extraction raw material and then pulverizing it as it is or by using a coarse crusher and subjecting it to extraction with an extraction solvent. At this time, the extraction raw material may be dried in the sun or using a commonly used dryer. Further, the above plants may be subjected to pretreatment such as defatting with a non-polar solvent such as hexane or benzene and then used as an extraction raw material.
By performing the pretreatment such as degreasing, the extraction treatment with the polar solvent can be efficiently performed.

As the extraction solvent, it is preferable to use water, a hydrophilic organic solvent or a mixture thereof at room temperature or at a temperature lower than the boiling point of the solvent.

The water that can be used as the extraction solvent includes pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water and the like, as well as those that have been subjected to various treatments.
Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering and the like.
Therefore, the water that can be used as the extraction solvent in the present invention also includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline and the like.

Examples of the hydrophilic organic solvent that can be used as the extraction solvent include, for example, methanol, ethanol, propyl alcohol, isopropyl alcohol and the like having 1 to 5 carbon atoms.
And lower aliphatic ketones such as acetone and methyl ethyl ketone; and polyhydric alcohols having 2 to 5 carbon atoms such as 1,3-butylene glycol, propylene glycol and glycerin.

When a mixed solution of two or more polar solvents is used as the extraction solvent, the mixing ratio can be adjusted appropriately. For example, when a mixed liquid of water and lower aliphatic alcohol is used, the mixing ratio of water and lower aliphatic alcohol can be 7: 3 to 2: 8 (weight ratio).

The extraction treatment is not particularly limited as long as the soluble component contained in the plant can be eluted in the extraction solvent, and can be carried out by a conventional method. It is not necessary to employ a special extraction method during the extraction treatment, and any device can be used at room temperature or under reflux heating.

Specifically, the extraction raw material is put into a treatment tank filled with an extraction solvent, and the mixture is usually left standing for 1 to 3 hours with stirring occasionally to elute soluble components and then filtered. The solid matter is removed, the extraction solvent is distilled off from the obtained extract, and the extract is obtained by drying. The extraction solvent amount is usually 5 to 15 times the extraction raw material amount (weight ratio), and the extraction temperature is usually room temperature to 95 ° C.

The obtained extract is diluted, concentrated, dried according to a conventional method in order to obtain a diluted solution or concentrated solution of the extracted solution, a dried product of the extracted solution, or a crudely purified product or a purified product thereof. You may perform processing, such as refinement | purification.

The obtained extract can be used as it is as a hyaluronic acid production promoter, but a concentrated solution or a dried product is easier to use. The above-mentioned plant extract can be formulated according to a conventional method. In the case of formulation, in order to facilitate storage and handling, dextrin, a pharmaceutically acceptable carrier such as cyclodextrin and any other auxiliaries can be added,
The extract from the above plant can be formulated into any dosage form such as powder, fruit granules and tablets.

Since the extract from the above plant has a peculiar odor, it is possible to carry out purification for the purpose of decolorization, deodorization, etc. within a range that does not impair its physiological activity. Since it is not used in a large amount when added to foods, foods and drinks, there is no practical problem even if it is unrefined. Specifically, the purification can be performed by activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like.

The hyaluronic acid production promoter of the present invention can activate the production of hyaluronic acid by fibroblasts, and prevent and / or improve the aging of the skin caused by the decrease or degeneration of hyaluronic acid.

[Skin cosmetics] The extract from the above plant is
Since it has an effect of preventing and / or improving skin aging, and has excellent usability and safety when applied to the skin, it is suitable for incorporation into skin cosmetics. The hyaluronic acid production promoter of the present invention may be added to the skin cosmetics.

There are no particular restrictions on the type of skin cosmetics to which the extracts from the above plants can be added, and specific examples thereof include ointments, creams, emulsions, lotions, packs, lipsticks, bath salts and the like.

The amount of the extract from the above plant in the skin cosmetic of the present invention can be appropriately adjusted depending on the kind of the skin cosmetic and the physiological activity of the extract. It is preferable that the content of the compound is about 0.00001 to 10% by weight in terms of the standard extract.

The skin cosmetic of the present invention contains various main agents and auxiliaries usually used in the production of skin cosmetics, and other optional aids, as long as they do not interfere with the hyaluronic acid production promoting action of the extract from the above plant. Agents can be used. The skin cosmetic of the present invention is not limited to the above-mentioned extract from the plant as the main ingredient, for prevention and improvement of skin aging.

[0028] In the skin cosmetic of the present invention, those which can be used as a skin cosmetic constituent together with the above-mentioned plant extract include, for example, astringents, bactericidal / antibacterial agents, whitening agents, UV absorbers, moisturizers. , Cell activating agents, anti-inflammatory / anti-allergic agents, antioxidants / active oxygen scavengers, etc., and when the above constituents are used in combination with the extract from the plant, the constituents used in combination with the extract from the plant The synergistic action between and may result in superior use effects that are normally expected.

In the case of producing a skin cosmetic containing the extract from the above plant, there is almost no restriction on the selection of other raw materials, and oils and fats, waxes, hydrocarbons, fatty acids, alcohols Any of general base materials and auxiliaries such as, esters, surfactants, and fragrances can be used.

[Beverage for Beauty] It has been confirmed that the extract from the above-mentioned plant has an action of preventing and / or improving skin aging and is not digested in the digestive tract. It is suitable for incorporation into any food or drink or dietary supplement. For food and drink and dietary supplements,
You may mix | blend the hyaluronic acid production promoter of this invention.

The food or drink containing the extract from the plant has an effect of preventing and / or improving skin aging and can be used as a cosmetic food or drink.
Here, the "food and drink for beauty" means a food and drink for the purpose of preventing and / or improving beautiful skin or aging of the skin.

The cosmetic food or drink of the present invention may be one in which the extract from the plant is mixed with any food or drink that does not interfere with its activity, or the extract from the plant is a main component. May be a dietary supplement.

When producing the cosmetic food or drink of the present invention,
For example, sugars such as dextrin and starch; gelatin,
Proteins such as soybean protein and corn protein;
Amino acids such as alanine, glutamine and isoleucine;
Polysaccharides such as cellulose and gum arabic; soybean oil, fats and oils such as medium-chain fatty acid triglyceride and the like can be added to prepare any dosage form.

The amount of the extract extracted from the above-mentioned plant in the cosmetic food or drink of the present invention is about 1 to 1 in total of the extract per adult in consideration of the general intake of the food or drink to be added.
It is suitable to make it about 1000 mg.

There are no particular restrictions on the type of food or drink that can contain the extract from the above-mentioned plant, but specific examples thereof include drinks such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks, lactic acid drinks (these drinks). Concentrated stock solution and powder for adjustment); frozen desserts such as ice cream, ice sorbet, shaved ice; noodles such as buckwheat, udon, harusame, gyoza rind, maimai rind, Chinese noodles, instant noodles; candy, chewing gum, candy , Sweets such as gum, chocolate, tablets, snacks, biscuits, jellies, jams, creams, baked goods; processed fish and livestock products such as kamaboko, ham, sausages; dairy products such as processed milk and fermented milk; salad oil, Oils and fats and processed foods such as tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing; sauces, Seasonings such as is; soups, stews, salads, prepared foods, pickles, and the like.

The hyaluronic acid production promoter, skin cosmetics and cosmetic food and drink of the present invention described above are suitably applied to humans, but as long as the respective effects are exhibited, It can also be applied to animals other than.

[0037]

EXAMPLES The present invention will be specifically described below with reference to production examples, test examples and formulation examples, but the present invention is not limited to the following examples.

[Manufacturing Example 1] 300 g of coarsely crushed leaves and branches of fumogusa, leaves of aerial grass, and seeds of Tsutsumi sea were added to 3000 mL of extraction solvent, and gently stirred for 2 hours. It was kept at 80 ° C. After performing the above extraction treatment using three kinds of extraction solvents of water, 50% ethanol or ethanol, the mixture was filtered, the filtrate was concentrated under reduced pressure at 40 ° C., and further dried by a reduced pressure dryer. Water as the extraction solvent, 50
Table 1 shows% ethanol and each extraction rate when ethanol was used. When the extraction solvent is a mixture, the mixing ratio is based on weight.

[0039] [Table 1] Extraction rate (wt%) Extraction leaves of leaves and branches 21.3 18.5 13.6 Marts land grass of raw materials water 50% ethanol ethanol吹雲grass 26.7 26 .4 7.5 Transoceanic Seeds 28.8 23.1 14.2

[Test Example 1] Hyaluronic acid production promoting action test 50% ethanol extract of the leaves and branches of the fume cloud obtained in Production Example 1, the leaves of the grassland grass, and the seeds of the ocean (hereinafter referred to as " The “sample”) was tested for hyaluronic acid production promoting action by the following test method.

Human normal neonatal fibroblast (NB1RGB) 1 ×
10 ^{6 of} 10% FBS in a 75 cm ² flask
In α-MEM medium (pH 7.2) containing 37 ° C, 5% C
O ₂ at under -95% air was cultured for 7 days. Cells were collected by trypsin treatment and α-MEM containing 1% FBS was used.
The medium was adjusted to 2.2 × 10 ⁴ cells / mL, 100 μL of each was seeded on a 96-well plate, and the mixture was incubated at 37 ° C., 5% CO
And cultured overnight under ₂ -95% air. Next day, sample (sample concentration: 50ppm or 200ppm) (ppm = μg
α-MEM medium containing 1% FBS in which 100 μL was added to each well at 37 ° C. and 5% CO
And cultured for 3 days under the ₂ -95% air.

10 μL of the culture supernatant was added to 90 μL of PBS (-)
Was diluted 10 times with 50 μL, and 50 μL thereof was added to an ELISA plate previously coated with hyaluronic acid, and ELISA was performed using various antibodies. Hyaluronic acid was quantified using a calibration curve. The hyaluronic acid production promoting rate was determined with the value when no sample was added as 100%.
The results are shown in Table 2.

[Table 2] Hyaluronic acid production promotion rate (%) Extraction raw material 200 ppm 50 ppm Foliar grass leaves and branches-316.1 Perennial grass leaves 188.8 239.2 Transoceanic seeds 155 .1 130.5

From the results shown in Table 2, all the 50% ethanol extracts of the leaves and branches of the fume cloud, the leaves of the upland grass, and the seeds of the large seaweed have a hyaluronic acid production promoting action, In particular, it was confirmed that the 50% ethanol extract of the leaves and branches of the cloud cloud had a large hyaluronic acid production promoting action. In addition, the hyaluronic acid production-promoting action of the 50% ethanol extraction part of the leaves and branches of the cloud cloud was 50 p
Even a very small amount of pm was large, so the addition amount was 200
The effect of promoting hyaluronic acid production in the case of ppm was not measured.

[Formulation Example 1] An emulsion having the following composition was produced by a conventional method. Fukuromogusa 50% ethanol extract 0.1 g Tabuchi grass 50% ethanol extract 0.1 g Tsutsumi 50% ethanol extract 0.1 g Jojoba oil 4 g Olive oil 2 g Squalane 2 g Cetanol 2 g Glyceryl monostearate 2 g Polyoxyethylene cetyl Ether (20E.0) 2.5g Polyoxyethylene sorbitan oleate (20E.0) 2g 1,3-Butylene glycol 3g Methyl paraoxybenzoate 0.15g Magnesium ascorbate phosphate 0.1g Dusk extract 0.1g Ginkgo biloba Leaf extract 0.1 g Conchiolin 0.1 g Oat extract 0.1 g Chamomile extract 0.1 g Dipotassium glycyrrhizinate 0.1 g Fragrance 0.05 g Purified water balance (total amount is 100 g)

[Formulation Example 2] A lotion having the following composition was produced by a conventional method. Mushroom herb water extract 0.2 g Fume cloud water extract 0.05 g Glycerin 3 g 1,3-butylene glycol 3 g Polyoxyethylene sorbitan oleate (20E.0) 0.5 g Methyl paraoxybenzoate 0.15 g Citric acid 0 .1 g sodium citrate 0.1 g oil-soluble licorice extract 0.1 g seaweed extract 0.1 g xylobiose mixture 0.5 g kujin extract 0.1 g fragrance 0.05 g purified water balance (total amount is 100 g)

[Formulation Example 3] A cream having the following composition was produced by a conventional method. Tsukai Taikai ethanol extract 0.1 g Tabizo grass ethanol extract 0.05 g Liquid paraffin 5 g Sarah beeswax 4 g Cetanol 3 g Squalane 10 g Lanolin 2 g Stearic acid 1 g Polyoxyethylene sorbitan oleate (20E.0) 1.5 g Glyceryl monostearate 3 g 1,3-butylene glycol 6 g Methyl paraoxybenzoate 1.5 g Yeast extract 0.1 g Perilla extract 0.1 g Linden extract 0.1 g Jiuyu extract 0.1 g Perfume 0.1 g Purified water balance (total amount 100 g And)

[Formulation Example 4] A pack having the following composition was produced by a conventional method. Fume cloud grass 50% ethanol extract 0.2 g Mushroom grass 50% ethanol extract 0.05 g Tsukai Taikai 50% ethanol extract 0.05 g Polyvinyl alcohol 15 g Polyethylene glycol 3 g Propylene glycol 7 g Ethanol 10 g Ethyl paraoxybenzoate 0.05 g Acetic acid Tocopherol 0.1 g Sage extract 0.1 g Touki extract 0.1 g Carrot extract 0.1 g Perfume 0.05 g Purified water Balance (total amount is 100 g)

[Formulation Example 5] The following mixture was tableted to produce a tablet-type dietary supplement. Fukimokusa water extract 1 part by weight Machiji grass water extract 1 part by weight Seawater extract 1 part by weight Powdered sugar (sucrose) 188 parts by weight Glycerin fatty acid ester 12 parts by weight

[Formulation Example 6] The following mixture was formed into granules to prepare a dietary supplement. Fukimo grass 50% ethanol extract 1 part by weight Ground grass 50% ethanol extract 1 part by weight Tsukai Taikai 50% ethanol extract 1 part by weight Beet oligosaccharide 1000 parts by weight Vitamin C 167 parts by weight Stevia extract 10 parts by weight

[0051]

The present invention provides a hyaluronic acid production promoter. The hyaluronic acid production promoter of the present invention is useful for preventing and / or improving skin aging. The present invention also provides a skin cosmetic having a hyaluronic acid production promoting action. The skin cosmetic of the present invention is
It has excellent usability and safety when applied to the skin, and is useful for preventing and / or improving aging of the skin. Further, according to the present invention, there is provided a food and drink product for beauty treatment to which a hyaluronic acid production promoting action is imparted. The cosmetic food and drink of the present invention is useful for preventing and / or improving skin aging.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A23L 2/52 A61K 7/00 K A61K 7/00 MN A61P 17/16 A61P 17/16 43/00 111 43/00 111 A23L 2/00 F (72) Inventor Masakumi Miyakoshi 1089-8 Shingata-cho, Ashina-gun, Hiroshima Prefecture Maruzen Pharmaceutical Co., Ltd. Research Institute F-term (reference) 4B017 LC03 LG15 LL09 LP01 4B018 LB08 LE01 LE02 MD07 MD61 ME14 MF01 4C083 AA032 AA082 AA111 AA112 AA122 AC022 AC072 AC102 AC122 AC182 AC242 AC302 AC422 AC442 AC482 AD042 AD112 AD212 AD412 AD512 AD532 AD642 AD662 4C088 AB12 AB46 AB54 AC01 AC03 AC04 AC05 AC11 BA08 BA10 CA06 CA63 MA07 NA07

Claims (3)

[Claims] 1. A hyaluronic acid production promoter comprising as an active ingredient an extract from one or two or more plants selected from the group consisting of Fukurokumo, Michijikusa and Tokaidaikai. 2. A skin cosmetic composition comprising an extract from one or two or more plants selected from the group consisting of Fukimogusa, Mushroom grass and Tsutsumi sea. 3. A food and drink product for beauty treatment, which comprises an extract from one or more plants selected from the group consisting of Fukimogusa, Mushokuji grass and Tsutsumi sea.