JP2006008571A - Moisturizer, antioxidant, antiaging agent, skin cosmetic and beauty drink and food - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a moisturizer, an atioxidant, a catalase production promoter, an agent for preventing or ameliorating cell injuries induced by hydrogen peroxide, an antiaging agent, a collagen production promoter, a skin cosmetic and beauty drink and food which are effective for preventing or ameliorating skin troubles, skin aging or various skin diseases associated with these. <P>SOLUTION: The moisturizer, the atioxidant, the catalase production promoter, the agent for preventing or ameliorating cell injuries induced by hydrogen peroxide, the antiaging agent, the collagen production promoter, the skin cosmetic and the beauty drink and food contain an extract of a flower of Averrhoa carambola L. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a moisturizing agent, an antioxidant, a catalase production promoter, a hydrogen peroxide cell disorder preventive / ameliorating agent, an anti-aging agent and a collagen production promoter, as well as an extraction from a plant. The present invention relates to skin cosmetics and beauty foods and drinks that contain products.

  There are various causes and onset mechanisms of various skin diseases associated with skin aging and rough skin, but the main causes are decreased skin moisturizing function, active oxygen generated in vivo, extracellular matrix components Reduction / denaturation, etc.

  When the moisture retention function of the skin is reduced due to external factors such as the environment and internal factors such as stress, the skin becomes dry and loses its elasticity and becomes bulky. Such dry skin may cause atopic dermatitis and various skin troubles, and is considered to cause a serious condition such as wrinkles. Therefore, it is considered that skin aging such as inflammation such as atopic dermatitis and rough skin, and formation of wrinkles can be prevented and improved by applying a moisturizing component to the skin tissue.

In recent years, active oxygen has attracted attention as a factor that oxidizes biological components, and adverse effects on living organisms have become a problem. Active oxygen is mainly generated in the process of energy metabolism in living cells, and is superoxide (that is, superoxide anion generated by one-electron reduction of oxygen molecules: .O ₂ ⁻ ), hydrogen peroxide (H ₂ O ₂ ). , Hydroxy radical (.OH) and singlet oxygen ( ¹ O ₂ ). These active oxygens are involved in the intracellular sterilization mechanism by phagocytic cells such as neutrophils and macrophages and play an important role in the removal of viruses and cancer cells, but excessive oxygen is generated Then, active oxygen may attack in vivo molecules constituting cell membranes and tissues and induce various diseases. For example, hydrogen peroxide is known to cause inflammation, production of lipid peroxide, inactivation of various enzymes, DNA damage, and the like.

  Normally, hydrogen peroxide is decomposed by hydrogen peroxide-degrading enzymes such as catalase, so that the concentration of hydrogen peroxide in the living body is kept low, and as a result, oxidative damage to cells and tissues caused by hydrogen peroxide is avoided. ing. However, since it is known that the production amount of catalase decreases with age, by promoting the production of catalase, excess hydrogen peroxide in the living body is decomposed to oxidize cells and tissues. It is thought that disability can be prevented and improved.

  Moreover, if cell damage caused by hydrogen peroxide can be prevented and improved to enhance cell homeostasis, it is considered that the turnover cycle of the skin tissue can be maintained normally. Therefore, it is considered that aging of the skin can be prevented or improved by promoting catalase production or preventing or improving cell damage caused by hydrogen peroxide.

  On the other hand, the epidermis and dermis of the skin are composed of epidermal cells, fibroblasts, and extracellular matrices such as elastin, hyaluronic acid, and collagen that are outside these cells and support the skin structure. In young skin, moisture retention, flexibility, elasticity and the like are ensured by maintaining the constant interaction of these skin tissues, and the skin is kept fresh and fresh with a firmness and gloss.

  However, the production of collagen, which is a major component of the extracellular matrix, is affected by certain external factors such as UV irradiation, drastic air drying, and excessive skin washing. Decreases and causes degradation and denaturation. As a result, the elasticity of the skin is lowered, the skin loses its tension, and the skin becomes rough and exhibits aging symptoms such as formation of spots and wrinkles.

  As described above, changes in the skin tissue accompanying aging, that is, formation of spots and wrinkles, disappearance of texture, decrease in elasticity, and the like involve reduction / denaturation of dermal matrix components such as collagen. Therefore, it is considered that aging of the skin can be prevented / improved by promoting the production of collagen.

Based on this idea, we have previously obtained substances that have moisturizing action, catalase production promoting action, prevention / amelioration of cell damage caused by hydrogen peroxide, or collagen production promoting action from natural products that are advantageous in terms of safety. Attempts have been made to extract, for example, an extract from the leaves of a quince (Patent Document 1), an extract from the fruit of a star fruit (Patent Document 2), an extract of Japanese maple (Patent Document 3), and a Kusunohagashi extract Effectiveness has been confirmed in products (Patent Document 4) and the like.
JP 2002-226323 A Japanese Patent Laid-Open No. 2003-300893 JP 2003-1113068 A JP 2003-146837 A

  The present invention has found a component having moisturizing action, catalase production promoting action, hydrogen peroxide cell disorder preventing / ameliorating action, collagen production promoting action from natural products, and a moisturizing agent containing the ingredient as an active ingredient, An object of the present invention is to provide an oxidant, a catalase production promoter, a hydrogen peroxide cell disorder preventive / improving agent, an anti-aging agent and a collagen production promoter, as well as skin cosmetics and cosmetic foods and drinks containing the above ingredients.

  In order to solve the above-mentioned problems, the moisturizing agent, antioxidant, catalase production promoter, hydrogen peroxide cell disorder preventing / ameliorating agent, anti-aging agent and collagen production promoter of the present invention are the following: Averrhoa carambola L .) It contains an extract from a flower part as an active ingredient.

  As used herein, “hydrogen peroxide cell damage” refers to cell damage caused by hydrogen peroxide, particularly oxidative damage such as inflammation, lipid peroxide production, inactivation of various enzymes, DNA damage, and the like. Meaning tissue disorders such as skin tissue disorders. Further, the cells are not particularly limited as long as they are living cells that are damaged by hydrogen peroxide.

  Moreover, the skin cosmetics and cosmetics and foods of the present invention are characterized in that an extract from the flower part of Averrhoa carambola L. is blended.

  It should be noted that the extract from the flower part of Averrhoa carambola L. has moisturizing action, catalase production promoting action, hydrogen peroxide cell damage prevention / improvement action and collagen production promoting action. It is not known at all to be effective in prevention and improvement, and this is a new finding of the present invention.

  According to the present invention, the moisturizing action of the extract from the quince flower part, the catalase production promoting action, the hydrogen peroxide cell damage preventing / ameliorating action and the collagen production promoting action based on the moisturizing effect, antioxidant effect and Excellent anti-aging effect, high safety moisturizer, antioxidant, catalase production promoter, hydrogen peroxide cell damage prevention / amelioration agent, anti-aging agent, collagen production promoter, skin cosmetics and cosmetics food and drink Can be provided.

[Humectant, antioxidant, catalase production promoter, hydrogen peroxide cell disorder preventive / ameliorating agent, anti-aging agent and collagen production promoter]
The moisturizer, antioxidant, catalase production promoter, hydrogen peroxide cell disorder preventive / improving agent, anti-aging agent and collagen production promoter of the present invention are the flower parts of Goshiko (scientific name: Averrhoa carambola L.). The extract from this is contained as an active ingredient.

  Here, the “extract from the flower part of the quince” includes the extract obtained from the flower part of the quince by the extraction process, the diluted or concentrated solution of the extract, and the extract. The obtained dried product, or any of these roughly purified products or purified products is included.

  The extraction raw material used in the present invention is the flower part of the quince. Here, the "flower" generally refers to the whole organ involved in sexual reproduction of a seed plant, and is composed of a flower leaf that is a leaf deformation and a flower axis that is a stem deformation. This includes organs such as pupae, petals, stamens, and heart skin. The “floral part” used as an extraction raw material in the present invention includes all of the organs involved in sexual reproduction of seed plants, as well as parts thereof, such as flower leaves, flower coats (buds and corolla), corolla, petals, etc. Is also included.

  Goshiko (scientific name: Averrhoa carambola L.) belongs to the family Oxalis, and fresh fruits are edible. Goshiko has been described in literature in China since the BC, and its fruit is also called a star fruit because of its star-shaped cross section. Gosho is cultivated in Okinawa, southeastern China, Yunnan and other tropical regions, and can be easily obtained from these regions.

  The flower part of the quince used as the raw material for extraction is suitably dried and pulverized immediately after collection. Drying may be performed in the sun or using a commonly used dryer. The flower part of pentagon may be used as an extraction raw material after pretreatment such as degreasing with a nonpolar solvent such as hexane. By performing a pretreatment such as degreasing, the extraction treatment with the polar solvent of the flower part of the quince can be performed efficiently.

  Details of the substance having moisturizing action, catalase production promoting action, hydrogen peroxide cell damage prevention / ameliorating action or collagen production promoting action contained in the extract from the flower part of the quince are unknown. By an extraction method generally used for extraction, an extract having a moisturizing action, a catalase production promoting action, a hydrogen peroxide cell disorder preventing / ameliorating action or a collagen production promoting action can be obtained.

  As the extraction solvent, it is preferable to use a polar solvent, and it is particularly preferable to use water, a hydrophilic organic solvent, or a mixture thereof at room temperature or a temperature not higher than the boiling point of the solvent. Substances with moisturizing action, catalase production promoting action, hydrogen peroxide cell damage prevention / ameliorating action or collagen production promoting action contained in the flower part of the quince are made of water, hydrophilic organic solvent or a mixture thereof. It can be easily extracted by an extraction process using an extraction solvent.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, and fresh water, and those that have been subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  Examples of the hydrophilic organic solvent that can be used as the extraction solvent include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, and propanol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene glycol, glycerin, Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as propylene glycol.

  When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when a mixed liquid of water and lower aliphatic alcohol is used, the mixing ratio of water and lower aliphatic alcohol can be set to 7: 3 to 2: 8 (volume ratio).

  An extraction process is not specifically limited as long as the soluble component contained in the flower part of a quince can be eluted in an extraction solvent, and can be performed according to a conventional method. In the extraction process, it is not necessary to adopt a special extraction method, and any apparatus can be used at room temperature or under reflux heating.

  For example, an extraction raw material (a quince flower part) is put into a treatment tank filled with an extraction solvent, and a soluble component is eluted with occasional stirring. At this time, the amount of the extraction solvent is usually 5 to 15 times (mass ratio) of the extraction raw material (the flower part of the quince), the extraction time is usually 1 to 3 hours, and the extraction temperature is usually from room temperature to It is preferable that it is 95 degreeC.

  An eluate can be obtained by eluting soluble components by extraction and then filtering to remove the extraction residue. In order to obtain a diluted or concentrated solution of the extract, a dried product of the extract, or a crude purified product or purified product thereof, the obtained extract is diluted, concentrated, dried, purified, etc. according to a conventional method. Processing may be performed.

  In addition, since the flower part of the quince has a unique odor and taste, it can be purified for the purpose of decolorization, deodorization, etc. within the range that does not cause a decrease in its physiological activity. When it is added to cosmetics or cosmetic foods and drinks, it is not used in large quantities, so there is no practical problem even if it is not purified. Specifically, purification can be performed by activated carbon treatment, adsorption resin treatment, ion exchange resin treatment, or the like.

  The extract obtained as described above has a moisturizing action, a catalase production promoting action, a hydrogen peroxide cell disorder preventing / ameliorating action or a collagen production promoting action, and using these actions, a moisturizing agent, It can be used as an antioxidant, a catalase production promoter, a hydrogen peroxide cell disorder preventive / ameliorating agent, an anti-aging agent or a collagen production promoter.

  The moisturizer, antioxidant, catalase production promoter, hydrogen peroxide cell disorder preventive / ameliorating agent, anti-aging agent or collagen production promoter may be composed only of the extract from the flower part of the above five lions. In addition, a product obtained by formulating an extract from the flower part of the above-mentioned quince may be used.

  The above-mentioned extract from the quince flower part is prepared by using a pharmaceutically acceptable carrier such as dextrin and cyclodextrin and other optional auxiliaries, tablets, capsules, powders, granules, syrups and troches. , Liquids, injections, suppositories, patches, coatings, eye drops, nasal drops, and the like. In this case, as an auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring / flavoring agent, and the like can be used.

  The moisturizing agent, antioxidant, catalase production promoter, hydrogen peroxide cell disorder preventive / ameliorating agent, anti-aging agent or collagen production promoter of the present invention is optionally moisturizing, catalase production promoting. In addition, other natural extracts having an effect of preventing or improving hydrogen peroxide cell damage or an effect of promoting collagen production can be blended and used as active ingredients.

  The moisturizing agent of the present invention can prevent and improve skin aging, rough skin, etc. through a moisturizing action on the skin of an extract from the flower part of the quince which is an active ingredient. However, the moisturizing agent of the present invention can be used for all uses other than these uses, which are meaningful for exhibiting a moisturizing action.

  In addition, the antioxidant of the present invention is produced by hydrogen peroxide through a catalase production promoting action and / or a hydrogen peroxide cell damage preventing / ameliorating action possessed by an extract from the flower part of the quince as an active ingredient. It can prevent or improve cell damage, tissue damage or skin aging. However, in addition to these uses, the antioxidant of the present invention can be used for all uses that are meaningful for exerting a catalase production promoting action and / or a hydrogen peroxide cell disorder preventing / ameliorating action.

  Furthermore, the catalase production promoter of the present invention can promote the production of catalase in vivo through the catalase production promoting action of the extract from the flower part of the quince which is an active ingredient. Thereby, catalase can decompose hydrogen peroxide generated in the living body, and oxidative damage of cells and tissues, for example, aging and canceration of cells can be prevented and improved. However, the catalase production promoter of the present invention can be used for all uses other than these uses, which are meaningful for exerting a catalase production promoting action.

  Furthermore, the preventive / ameliorating agent for hydrogen peroxide cell damage of the present invention is effective for the prevention of cell or tissue through the action of preventing or improving hydrogen peroxide cell damage possessed by the extract from the flower part of the quince as an active ingredient. It can prevent and improve oxidative damage and skin aging. Here, the effect of preventing or improving hydrogen peroxide cell damage may be exhibited based on the catalase production promoting effect, or may be exhibited based on other effects. . However, the preventive / ameliorating agent for hydrogen peroxide cell damage of the present invention can be used for all uses other than these uses, which are meaningful to exhibit the action of preventing and improving hydrogen peroxide cell damage. .

  The anti-aging agent of the present invention can prevent and improve skin aging through the collagen production promoting action of the extract from the flower part of the quince which is an active ingredient. However, the anti-aging agent of the present invention can be used for all uses other than these uses, which are meaningful for exerting a collagen production promoting action.

  Moreover, the collagen production promoter of this invention can accelerate | stimulate collagen production by the collagen production promotion effect | action which the extract from the flower part of the quince which is an active ingredient has. Thereby, the reduction | decrease and degeneration | denaturation of collagen which is a main structural component of a dermal matrix can be suppressed, and skin aging can be prevented and improved. However, the collagen production promoter of the present invention can be used for all uses other than these uses, which are meaningful for exerting a collagen production promoting effect.

[Skin cosmetic]
Since the extract from the flower part of the above-mentioned quince has excellent usability and safety when applied to the skin, it is suitable for blending into skin cosmetics. In this case, an extract from the flower part of the quince may be added as it is, or a moisturizer, an antioxidant, a catalase production promoter, a hydrogen peroxide cell disorder prevention / improvement formulated from the extract. You may mix | blend an agent, an anti-aging agent, or a collagen production promoter. In addition, when a moisturizer, an antioxidant, a catalase production promoter, a hydrogen peroxide cell disorder preventive / improving agent, an anti-aging agent or a collagen production promoter is added to skin cosmetics, one of those agents. You may mix | blend and may mix | blend 2 or more types in combination.

  Skin cosmetics that can be blended with the extract from the flower part of the quince are not particularly limited. Specific examples thereof include ointments, creams, emulsions, lotions, packs, jellies, lip balms, lipsticks, bathing agents, etc. Is possible.

  The blending amount of the extract from the quince flower part in the skin cosmetic of the present invention can be adjusted as appropriate depending on the type of skin cosmetic and the physiological activity of the extract. It is about 0.001 to 10% by mass in terms of an extract from a typical flowering part of a quince.

  The skin cosmetic of the present invention is a skin cosmetic as long as it does not interfere with the moisturizing action, catalase production promoting action, hydrogen peroxide cell disorder preventing / ameliorating action or collagen production promoting action possessed by the extract from the quince flower part. Main agents or auxiliaries used in the normal production of the additives, such as astringents, bactericides / antibacterial agents, whitening agents, ultraviolet absorbers, humectants, cell activators, etc. can be used in combination. By using in combination, it becomes a more general product, and thereby a synergistic effect with other active ingredients used in combination may lead to a superior effect than would normally be expected.

[Beauty food and drink]
The above-mentioned extract from the flower part of the quince has moisturizing action, catalase production promoting action, hydrogen peroxide cell damage preventing / ameliorating action or collagen production promoting action, and is digested in the digestive tract. Since it has been confirmed that it is not a thing, and it is excellent in safety, it is suitable for blending into cosmetic foods and drinks. In this case, an extract from the quince flower part may be blended as it is, or a moisturizer, antioxidant, catalase production promoter, excess An agent for preventing / ameliorating hydrogen oxide cell damage, an anti-aging agent, or a collagen production promoter may be added.

  Here, “beauty food and drink” refers to food and drink intended for beautifying the skin, or food and drink intended to prevent and improve rough skin, aging of the skin and various skin diseases associated therewith. Means.

  Extracts from the flower parts of the above five lions, or moisturizers, antioxidants, catalase production promoters, hydrogen peroxide cell damage preventive / improving agents, anti-aging agents or collagen production promoters into cosmetic foods and drinks When blended, the compounding amount of the active ingredient in them can be appropriately adjusted in consideration of the purpose of use, sex, symptoms, etc., but in terms of the extract from the standard quince flower part, It is preferable to be about 0.01 to 10% by mass with respect to the mass of the food or drink to be added.

  The beauty food and drink of the present invention may be an extract obtained from the flower part of the quince in any food or drink that does not impede its activity, and the extract is the main component. It may be a dietary supplement.

  When producing the cosmetic food and drink of the present invention, for example, sugars such as dextrin and starch; proteins such as gelatin, soybean protein and corn protein; amino acids such as alanine, glutamine and isoleucine; cellulose and gum arabic Polysaccharides: Foods and drinks of any shape can be made by adding arbitrary auxiliaries such as oils and fats such as soybean oil and medium chain fatty acid triglycerides.

  The foods and drinks that can be blended with the extract from the flower part of the quince are not particularly limited. Concentrated undiluted solution and preparation powder); Ice cream, ice sherbet, shaved ice and other frozen desserts; buckwheat, udon, harusame, gyoza skin, cucumber skin, Chinese noodles, instant noodles and other noodles; rice cake, chewing gum, candy, Sweets such as gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery; processed fishery and livestock products such as kamaboko, ham, sausage; dairy products such as processed milk, fermented milk; salad oil, tempura Oils and fats such as oil, margarine, mayonnaise, shortening, whipped cream, dressing and processed foods; seasonings such as sauces and sauces Soups, stews, salads, prepared dishes, pickles; various other forms of health and nutritional supplements; tablets, capsules, drinks, etc. When blending, commonly used auxiliary raw materials and additives can be used in combination.

  The humectant, antioxidant, catalase production promoter, hydrogen peroxide cell disorder preventive / improving agent, anti-aging agent, collagen production promoter, skin cosmetics and cosmetic foods and beverages of the present invention described above are for humans. However, as long as each effect is exhibited, it can be applied to animals other than humans.

  Hereinafter, although a manufacture example, a test example, and a compounding example are shown and this invention is demonstrated concretely, this invention is not limited to each following example at all.

[Production Example 1]
100 g of the coarsely pulverized flower of the quince flower part was put into 1000 mL of an extraction solvent, and heat extraction was performed for 3 hours at a temperature of 80 ° C. Thereafter, the mixture was filtered, and the filtrate was concentrated under reduced pressure and further dried to obtain an extract from the flower part of the quince. The yield of each extract when water, 50% ethanol (volume ratio of water to ethanol = 1: 1) and 80% ethanol (volume ratio of water to ethanol = 1: 4) were used as extraction solvents. Table 1 shows.

[Table 1]
Sample extraction solvent extract yield (%)
1 Water 13.4
2 50% ethanol 18.9
3 80% ethanol 14.2

[Test Example 1] Moisturizing Action Test Samples 1 to 3 obtained in Production Example 1 were tested for moisturizing action by the following test method.

A 0.05% by mass aqueous solution of the extract of samples 1 to 3 (sample solutions 1 to 3), 1% glycerin (sample solution 4), and purified water (sample solution 5) were each a paper disk having a diameter of 8 mm (manufactured by Toyo Seisakusho). , Mass: about 0.017 g). These paper discs were left in the test chamber, and the mass of the paper discs was measured every minute until 8 minutes passed. The water residual ratio (%) of each sample solution was determined with the mass at 0 minute being 100%. The test was performed under conditions of a room temperature of 23 ° C. and a humidity of 55%.
Table 2 shows the results of the above test.

[Table 2] Moisture residual rate (%)
Hours (minutes)
Sample solution 0 1 2 3 4 5 6 7 8
1 100.0 91.8 86.6 81.4 76.3 71.1 66.0 60.8 55.7
2 100.0 92.3 87.5 82.7 76.9 72.1 67.3 62.5 57.7
3 100.0 94.2 89.3 83.5 78.6 73.8 68.9 64.1 59.2
4 100.0 95.6 90.1 84.6 79.1 73.6 68.1 62.6 57.1
5 100.0 93.8 87.7 81.5 75.3 69.1 63.0 56.8 50.6

  As shown in Table 2, all of the sample solutions (sample solutions 1 to 3) containing the extract from the flower part of the quince have a high water residual ratio compared to purified water (sample solution 5), and glycerin. The moisture remaining rate was almost the same as that of the sample solution containing (humectant) (sample solution 4). From this result, it was confirmed that the extract from the flower part of the quince has an excellent moisturizing action.

[Test Example 2] Catalase production promoting action test Samples 1 to 3 obtained in Production Example 1 were tested for catalase production promoting action by the following test method.

Human normal skin fibroblasts (NB1RGB) were cultured using α-MEM medium containing 10% FBS, and then cells were collected by trypsin treatment. The collected cells are diluted with α-MEM culture solution containing 5% FBS so that the cell concentration becomes 4.0 × 10 ⁵ cells / mL, and then seeded at 200 μL per well in a 48-well plate. Cultured overnight.

  After completion of the culture, the culture solution was removed, and 200 μL of a sample solution (sample concentration: 100 μg / mL) in which the sample was dissolved in α-MEM culture solution containing 1% FBS was added to each well and cultured for 24 hours. After culturing, ultrasonic disruption was performed in PBS (−), and the amount of catalase present in the cell disruption solution was measured by an indirect ELISA method.

  At the same time, the amount of total protein in normal human skin fibroblasts was quantified, and the amount of catalase per amount of total protein was determined. The catalase production promotion rate per total protein amount at the time of sample addition was calculated by the following formula.

Catalase production promotion rate (%) = A / B × 100
In the above formula, “A” indicates “a catalase amount per total protein amount when a sample is added”, and “B” indicates “a catalase amount per total protein amount when no sample is added”.
The results of the above test are shown in Table 3.

[Table 3]
Sample catalase production promotion rate (%)
1 101.0
2 106.8
3 173.1

  As shown in Table 3, it was confirmed that the extract from the flower part of the quince has an excellent catalase production promoting action.

[Test Example 3] Hydrogen peroxide cell damage prevention / amelioration action test Samples 1 to 3 obtained in Production Example 1 were tested for the hydrogen peroxide cell damage prevention / amelioration action by the following test method.

1 × 10 ⁶ human normal dermal fibroblasts (NB1RGB) in an 80 cm ² flask using an α-MEM culture solution (pH: 7.2) containing 10% FBS at 37 ° C., 5% CO _2. Pre-cultured for 5 days under -95% air conditions. Pre-cultured cells were collected by trypsin treatment, adjusted to a cell concentration of 2.5 × 10 ⁵ cells / mL using α-MEM culture solution containing 5% FBS, and 200 μL per well in a 48-well microplate. The aliquots were aliquoted and cultured overnight at 37 ° C. and 5% CO ₂ -95% air.

After the culture, the culture solution is removed, and 200 μL of each sample solution (sample concentration: 400 μg / mL) dissolved in α-MEM culture solution containing 1% FBS is added to each well at 37 ° C., 5 ° C. They were cultured for 24 hours under the conditions of% _CO 2 -95% air. After culturing, the plate was washed with 400 μL of PBS (−), 200 μL of Hank's buffer solution (hydrogen peroxide final concentration: 1 mM) dissolved in hydrogen peroxide was added, and 37 ° C., 5% CO ₂ -95% air. It was cultured for 2 hours under the conditions.

  In addition, 200 μL of Hank's buffer solution in which hydrogen peroxide was not dissolved was added and cultured under the same conditions. Furthermore, it culture | cultivated without adding a sample solution, and after culture | cultivation, 200 microliters of Hank's buffer solution which melt | dissolved hydrogen peroxide was added, and it culture | cultivated on the same conditions.

After culturing, 200 μL of 0.05 mg / mL neutral red solution dissolved in α-MEM culture solution containing 1% FBS and washed with 400 μL of PBS (−) was added at 37 ° C., 5% CO _2. The cells were cultured for 2.5 hours under -95% air conditions. Thereafter, the neutral red solution was removed, and 300 μL of an ethanol / acetic acid solution (ethanol: acetic acid: water = 50: 1: 49) was added to each well to extract the dye. Thereafter, the absorbance at 540 nm was measured using a microplate reader to determine the hydrogen peroxide disorder inhibition rate (%). In addition, the hydrogen peroxide disorder inhibition rate was calculated by the following formula.

Hydrogen peroxide damage inhibition rate (%) = {(Nt−C) − (Nt−Sa)} / (Nt−C) × 100
In the above formula, “Nt” is the absorbance of “no sample solution added / hydrogen peroxide solution added”, “C” is the absorbance of “no sample solution added / hydrogen peroxide solution added”, and “Sa” is “ The absorbance of “sample solution addition / hydrogen peroxide solution addition” is shown.
Table 4 shows the results of the above test.

[Table 4]
Sample Hydrogen peroxide damage inhibition rate (%)
1 9.4
2 17.7
3 16.8
No sample added 0

  As shown in Table 4, all the extracts (samples 1 to 3) from the flower part of the quince showed a high hydrogen peroxide damage inhibition rate. From this result, it was confirmed that the extract from the flower part of the quince has an excellent effect of preventing and improving hydrogen peroxide cell damage.

[Test Example 4] Collagen production promoting action The samples 1 to 3 obtained in Production Example 1 were tested for collagen production promoting action by the following test method.

Human fibroblasts are seeded in a 96-well plate and cultured in a sample-added medium (sample concentration: 200 μg / mL) for several days under conditions of 37 ° C. and 5% CO ₂ -95% air. 90 μL of the supernatant was transferred to an ELISA plate and adsorbed on the plate overnight at a temperature of 4 ° C. Then, the solution was removed, and a phosphate physiological buffer solution containing 0.05% Tween-20 ( Washing was performed with PBS-T).

  After washing, blocking operation was performed with phosphate physiological buffer containing 1% bovine serum albumin, the solution was removed, and washed with phosphate physiological buffer (PBS-T) containing 0.05% Tween-20. And reacted with an anti-human collagen type I antibody (rabbit IgG antibody, manufactured by Chemicon). The solution was removed, washed with phosphate physiological buffer (PBS-T) containing 0.05% Tween-20, reacted with HRP-labeled anti-rabbit IgG antibody, the same washing operation was performed, and color development Reaction was performed.

Collagen production acceleration rate is determined by performing the above ELISA method using a standard product, creating a calibration curve from the results, obtaining the collagen production amount when the sample is added and the collagen production amount when no sample is added, The amount of collagen production without addition was calculated as 100%.
The results of the above test are shown in Table 5.

[Table 5]
Sample collagen production promotion rate (%)
1 230.9
2 322.5
3 224.1

  As shown in Table 5, it was confirmed that the extract from the flower part of the quince has an excellent collagen production promoting effect.

[Formulation Example 1]
A lotion having the following composition was produced by a conventional method.
Goshiko flower part water extract (Production Example 1) 1.0 g
1,3-butylene glycol 4.0 g
Glycerin 4.0g
Dipropylene glycol 2.0g
Five ginseng leaves 50% ethanol extract 1.0 g
Chamomile extract 0.5g
Gennoshoco extract 0.1g
Polyoxyethylene polyoxypropylene
Decyl tetradecyl ether 0.7g
Ethanol 3.0g
Perfume appropriate amount methyl paraoxybenzoate 0.15g
Purified water remainder (total amount is 100 g)

[Formulation Example 2]
A serum having the following composition was produced by a conventional method.
Goshiko flower part 80% ethanol extract (Production Example 1) 1.0 g
Carboxyvinyl polymer 0.2g
Xanthan gum 0.2g
Sodium hyaluronate 0.1g
Polyethylene glycol 3.0g
Jaundice extract 0.5g
Carrot extract 1.0g
Glycerin 6.0g
1,3-butylene glycol 3.0 g
Methyl paraoxybenzoate 0.15g
L-Arginine 0.15g
Purified water remainder (total amount is 100 g)

[Composition Example 3]
A cream having the following composition was produced by a conventional method.
Penta flower 50% ethanol extract (Production Example 1) 1.0 g
Polyethylene glycol monostearate 2.0g
Self-emulsifying glyceryl monostearate 5.0 g
Stearic acid 2.0g
Cetanol 2.0g
Perilla extract 1.0g
Rosemary extract 1.0g
Squalane 12.0g
Macadamia nut oil 3.0g
0.2g of methylpolysiloxane
Perfume appropriate amount methyl paraoxybenzoate 0.15g
1,3-butylene glycol 7.0 g
Xanthan gum 0.2g
Purified water remainder (total amount is 100 g)

[Formulation Example 4]
The following raw materials were uniformly mixed, granulated by a conventional method, and then tableted to produce a tablet-shaped dietary supplement.
50.0 grams of water extract from Goshiko flower (Production Example 1)
Maltitol 47.0g
15.0 g of crystalline cellulose
Sucrose fatty acid ester 8.0g

[Formulation Example 5]
The following ingredients were mixed uniformly and formed into granules to produce a dietary supplement.
Penta flower part 50% ethanol extract (Production Example 1) 34 parts by weight Beet oligosaccharide 1000 parts by weight Vitamin C 167 parts by weight Stevia extract 10 parts by weight

The moisturizing agent, antioxidant, catalase production promoter, preventive / improving agent for tissue damage due to hydrogen peroxide, anti-aging agent and collagen production promoter, skin cosmetics and cosmetics for food and drink of the present invention are rough skin, It is useful for preventing and improving skin aging such as wrinkle formation.

Claims (8)

  A moisturizing agent characterized by containing an extract from the flower part of a gooseberry (Averrhoa carambola L.) as an active ingredient.   An antioxidant comprising an extract from the flower part of Averrhoa carambola L. as an active ingredient.   A catalase production promoter characterized by containing, as an active ingredient, an extract from the flower part of Averrhoa carambola L ..   A prophylactic / ameliorating agent for hydrogen peroxide cell damage, characterized by containing an extract from the flower part of Averrhoa carambola L. as an active ingredient.   An anti-aging agent characterized by containing an extract from the flower part of a gourd (Averrhoa carambola L.) as an active ingredient.   Collagen production promoter characterized by containing an extract from the flower part of a gourd (Averrhoa carambola L.) as an active ingredient.   Skin cosmetic characterized by containing an extract from the flower part of Averrhoa carambola L. A beauty food and drink characterized by containing an extract from the flower part of Averrhoa carambola L.