JP2014114235A - Melanogenesis inhibitor and collagen production promoter - Google Patents
Melanogenesis inhibitor and collagen production promoter Download PDFInfo
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- JP2014114235A JP2014114235A JP2012268933A JP2012268933A JP2014114235A JP 2014114235 A JP2014114235 A JP 2014114235A JP 2012268933 A JP2012268933 A JP 2012268933A JP 2012268933 A JP2012268933 A JP 2012268933A JP 2014114235 A JP2014114235 A JP 2014114235A
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- extract
- pineapple
- collagen production
- collagen
- skin
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Abstract
Description
本発明は、メラニン産生抑制剤、コラーゲン産生促進剤、皮膚化粧料及び飲食品に関するものである。 The present invention relates to a melanin production inhibitor, a collagen production promoter, a skin cosmetic, and a food and drink.
皮膚においてメラニンは、紫外線から生体を保護する役目も果たしているが、過剰生成や不均一な蓄積は、皮膚の黒化やシミの原因となる。一般にメラニンは、色素細胞の中で生合成される酵素チロシナーゼの働きによって、チロシンからドーパ、ドーパからドーパキノンに変化し、ついで５，６−ジヒドロキシインドフェノール等の中間体を経て形成されるものとされている。したがって、皮膚の色黒（皮膚色素沈着症）、シミ、ソバカス等を予防、治療又は改善するためには、メラニンの産生を抑制することが考えられる。 Melanin also plays a role in protecting the body from ultraviolet rays in the skin, but overproduction and uneven accumulation cause skin darkening and spots. In general, melanin is converted from tyrosine to dopa and from dopa to dopaquinone by the action of the enzyme tyrosinase biosynthesized in pigment cells, and then formed through intermediates such as 5,6-dihydroxyindophenol. ing. Therefore, in order to prevent, treat, or improve skin darkness (skin pigmentation), spots, buckwheat, etc., it is conceivable to suppress the production of melanin.
このような観点から、従来、メラニン産生抑制作用を有するものとして、例えば、トウゴマ根部からの抽出物（特許文献１参照）、サウスウレア（Saussurea）属に属する植物からの抽出物（特許文献２参照）等が知られている。 From such a point of view, conventionally, as one having an inhibitory action on melanin production, for example, an extract from roots of castor bean (see Patent Document 1), an extract from a plant belonging to the genus Southus (Saussurea) (see Patent Document 2) Etc. are known.
皮膚は、表皮、基底膜及び真皮から構成されている。真皮は、線維芽細胞と該細胞から分泌されるコラーゲン、エラスチン及びヒアルロン酸等の細胞外マトリックスとにより構成されている。若い皮膚においてはこれらの皮膚組織が恒常性を維持することにより水分保持力、柔軟性、弾力性等が確保され、肌は外見的にも張りや艶があってみずみずしい状態に維持される。 The skin is composed of the epidermis, basement membrane and dermis. The dermis is composed of fibroblasts and an extracellular matrix such as collagen, elastin and hyaluronic acid secreted from the cells. In young skin, these skin tissues maintain their homeostasis, thereby ensuring moisture retention, flexibility, elasticity, and the like, and the skin is kept fresh and fresh with a firmness and gloss.
ところが、皮膚が紫外線、著しい空気の乾燥、過度の皮膚洗浄、ストレス、喫煙等の外的因子の影響を受けたり、加齢が進んだりすると、細胞外マトリックスの主要構成成分であるコラーゲン等の産生量が減少するとともに、分解や変質を引き起こす。その結果、皮膚の保湿機能や弾力性が低下し、角質の異常剥離が起こり、皮膚の老化に伴う変化、すなわち、シワ、くすみ、きめの消失、弾力性の低下等が生じる。 However, when the skin is affected by external factors such as ultraviolet rays, significant air drying, excessive skin washing, stress, smoking, etc., or when aging progresses, production of collagen, which is a major component of the extracellular matrix, is produced. As the amount decreases, it causes degradation and alteration. As a result, the moisturizing function and elasticity of the skin are reduced, abnormal exfoliation of the keratin occurs, and changes accompanying aging of the skin, that is, wrinkles, dullness, disappearance of texture, reduced elasticity, and the like occur.
このように、皮膚の老化に伴う変化、すなわち、シワの形成、張りの消失、弾力性の低下等には、コラーゲン等の細胞外マトリックス成分の減少、変性が関与している。したがって、コラーゲンの産生を促進することは、皮膚の老化を防止又は改善する上で重要である。 As described above, changes associated with aging of the skin, that is, formation of wrinkles, loss of tension, decrease in elasticity, and the like are associated with reduction and degeneration of extracellular matrix components such as collagen. Therefore, promoting the production of collagen is important in preventing or improving skin aging.
また、コラーゲンは、骨、腱、靱帯、角膜、血管等にも多く存在し、加齢等によるコラーゲン産生の低下が、骨粗鬆症等の原因になることが知られている。さらに、創傷の治癒過程において、コラーゲンの産生量が亢進し、線維芽細胞等の足場となることで、創傷の治癒を促進することが知られている。そのため、コラーゲンの産生を促進することは、骨粗鬆症等の予防又は治療、創傷治癒の促進といった観点からも重要である。 Collagen is also present in a large amount in bones, tendons, ligaments, corneas, blood vessels and the like, and it is known that a decrease in collagen production due to aging or the like causes osteoporosis and the like. Furthermore, it is known that in the wound healing process, the amount of collagen produced is increased and becomes a scaffold for fibroblasts to promote wound healing. Therefore, promoting the production of collagen is important from the viewpoint of preventing or treating osteoporosis and the like, and promoting wound healing.
従来、コラーゲン産生促進作用を有するものとしては、例えば、五斂子の葉部からの抽出物（特許文献３参照）、クスノハガシワからの抽出物（特許文献４参照）等が知られている。 Conventionally, what has a collagen production promotion effect is known, for example, an extract from a leaf part of a quince (see Patent Document 3), an extract from Kusunohagashi (see Patent Document 4), and the like.
本発明は、安全性の高い天然物の中からメラニン産生抑制作用又はコラーゲン産生促進作用を有するものを見出し、それを有効成分とするメラニン産生抑制剤及びコラーゲン産生促進剤、並びに当該天然物を配合した皮膚化粧料及び飲食品を提供することを目的とする。 The present invention finds a highly safe natural product having a melanin production inhibitory effect or a collagen production promoting effect, and contains a melanin production inhibitor and a collagen production promoter containing the natural product as an active ingredient. It aims at providing the skin cosmetics and food / beverage products which were made.
上記課題を解決するために、本発明のメラニン産生抑制剤及びコラーゲン産生促進剤は、パイナップルからの抽出物とキンモクセイからの抽出物とを有効成分として含有することを特徴とする。また、本発明の皮膚化粧料及び飲食品は、パイナップルからの抽出物とキンモクセイからの抽出物とを配合したことを特徴とする。 In order to solve the above-mentioned problems, the melanin production inhibitor and the collagen production promoter of the present invention are characterized by containing an extract from pineapple and an extract from betel grass as active ingredients. Moreover, the skin cosmetics and food / beverage products of this invention mix | blended the extract from a pineapple and the extract from a quince mushroom.
本発明によれば、優れたメラニン産生抑制作用を有し、かつ安全性の高いメラニン産生抑制剤、及び優れたコラーゲン産生促進作用を有し、かつ安全性の高いコラーゲン産生促進剤を提供することができる。 According to the present invention, there are provided a highly safe melanin production inhibitor having an excellent melanin production inhibitory action, and a highly safe collagen production promoter having an excellent collagen production promotion action and high safety. Can do.
以下、本発明の実施の形態について説明する。
本実施形態のメラニン産生抑制剤及びコラーゲン産生促進剤は、パイナップルからの抽出物とキンモクセイからの抽出物とを有効成分として含有するものである。また、本実施形態の皮膚化粧料及び飲食品は、パイナップルからの抽出物とキンモクセイからの抽出物とが配合されるものである。
Embodiments of the present invention will be described below.
The melanin production inhibitor and the collagen production promoter of this embodiment contain an extract from pineapple and an extract from betel grass as active ingredients. Moreover, the skin cosmetics and food / beverage products of this embodiment mix | blend the extract from a pineapple and the extract from a quince mushroom.
ここで、本実施形態における「抽出物」には、上記植物を抽出原料として得られる抽出液、当該抽出液の希釈液若しくは濃縮液、当該抽出液を乾燥して得られる乾燥物、又はこれらの粗精製物若しくは精製物のいずれもが含まれる。 Here, the “extract” in the present embodiment includes an extract obtained using the above plant as an extraction raw material, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, or these Either a crude product or a purified product is included.
本実施形態において、パイナップルからの抽出物を製造するために使用する抽出原料は、パイナップル（学名：Ananas comosus (L.) Merr.又はAnanas sativus Schult）である。 In this embodiment, the extraction raw material used in order to manufacture the extract from a pineapple is a pineapple (scientific name: Ananas comosus (L.) Merr. Or Ananas sativus Schult).
パイナップル（Ananas comosus (L.) Merr.又はAnanas sativus Schult）は、熱帯アメリカ原産のパイナップル科アナナス属に属する多年生の植物である。パイナップルは、タイ、フィリピン、ブラジルなどの熱帯から亜熱帯で広く栽培されており、これらの地域から容易に入手することができる。抽出原料として使用し得るキンモクセイの構成部位としては、例えば、葉部、茎部、果実部、花部等の地上部、根部、地下茎部等の地下部、又はこれらの部位の混合物等が挙げられるが、好ましくは果実部である。 Pineapple (Ananas comosus (L.) Merr. Or Ananas sativus Schult) is a perennial plant belonging to the genus Ananas of the pineapple family native to tropical America. Pineapples are widely cultivated from tropical to subtropical regions such as Thailand, the Philippines, and Brazil, and can be easily obtained from these regions. Examples of the constituent parts of the umbilical cane that can be used as the raw material for extraction include the above-ground parts such as leaf parts, stem parts, fruit parts, flower parts, underground parts such as root parts, underground stem parts, or a mixture of these parts. However, it is preferably a fruit part.
また、本実施形態において、キンモクセイからの抽出物を製造するために使用する抽出原料は、キンモクセイ（学名：Osmanthus fragrans var. aurantiacus）である。 Moreover, in this embodiment, the extraction raw material used in order to manufacture the extract from Buttercups is a Butterflies (Scientific name: Osmanthus fragrans var. Aurantiacus).
キンモクセイ（Osmanthus fragrans var. aurantiacus）は、モクセイ科モクセイ属に属する常緑小高木であり、別名、桂花とも呼ばれ、中国南部が原産地であり、このような地域から容易に入手することができる。抽出原料として使用し得るキンモクセイの構成部位としては、例えば、葉部、枝部、樹皮部、幹部、茎部、果実部、花部等の地上部、根部又はこれらの部位の混合物等が挙げられるが、好ましくは花部である。 Buttercup (Osmanthus fragrans var. Aurantiacus) is an evergreen small tree belonging to the genus Moxae, also known as Katsura, which is native to southern China and can be easily obtained from such areas. Examples of the constituent parts of the cypress that can be used as the raw material for extraction include the aerial parts such as leaves, branches, bark parts, trunk parts, stem parts, fruit parts, flower parts, root parts, or a mixture of these parts. However, it is preferably a flower part.
ここで、「花」とは、一般に、種子植物の有性生殖にかかわる器官の総体をいい、葉の変形である花葉と茎の変形である花軸とから構成され、花葉には、萼、花弁、雄しべ、心皮等の器官が含まれる。本発明において抽出原料として使用する「花部」には、種子植物の有性生殖にかかわる器官の総体の他、その一部、例えば、花葉、花被（萼と花冠）、花冠、花弁等も含まれる。 Here, the "flower" generally refers to the whole organ involved in sexual reproduction of a seed plant, and is composed of a flower leaf that is a leaf deformation and a flower axis that is a stem deformation. This includes organs such as pupae, petals, stamens, and heart skin. The “floral part” used as an extraction raw material in the present invention includes all of the organs involved in sexual reproduction of seed plants, as well as parts thereof, such as flower leaves, flower coats (buds and corolla), corolla, petals, etc. Is also included.
パイナップルからの抽出物及びキンモクセイからの抽出物は、抽出原料を乾燥した後、そのまま又は粗砕機を用いて粉砕し、抽出溶媒による抽出に供することにより得ることができる。乾燥は天日で行ってもよいし、通常使用される乾燥機を用いて行ってもよい。また、ヘキサン等の非極性溶媒によって脱脂等の前処理を施してから抽出原料として使用してもよい。脱脂等の前処理を行うことにより、上記抽出原料の極性溶媒による抽出処理を効率よく行うことができる。 The extract from pineapple and the extract from cinnamon can be obtained by drying the raw material for extraction and pulverizing it as it is or using a crusher, and subjecting it to extraction with an extraction solvent. Drying may be performed in the sun or using a commonly used dryer. Moreover, after performing pretreatment, such as degreasing, with a nonpolar solvent such as hexane, it may be used as an extraction raw material. By performing a pretreatment such as degreasing, the extraction material can be efficiently extracted with a polar solvent.
抽出溶媒としては、極性溶媒を使用することが好ましく、例えば、水、親水性有機溶媒等が挙げられ、これらを単独で又は２種以上を組み合わせて、室温又は溶媒の沸点以下の温度で使用することが好ましい。 As the extraction solvent, it is preferable to use a polar solvent, and examples thereof include water and hydrophilic organic solvents. These are used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. It is preferable.
抽出溶媒として使用し得る水としては、純水、水道水、井戸水、鉱泉水、鉱水、温泉水、湧水、淡水等のほか、これらに各種処理を施したものが含まれる。水に施す処理としては、例えば、精製、加熱、殺菌、濾過、イオン交換、浸透圧調整、緩衝化等が含まれる。したがって、本実施形態において抽出溶媒として使用し得る水には、精製水、熱水、イオン交換水、生理食塩水、リン酸緩衝液、リン酸緩衝生理食塩水等も含まれる。 Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, and those subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, and the like. Therefore, the water that can be used as the extraction solvent in this embodiment includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.
抽出溶媒として使用し得る親水性有機溶媒としては、メタノール、エタノール、プロピルアルコール、イソプロピルアルコール等の炭素数１〜５の低級脂肪族アルコール；アセトン、メチルエチルケトン等の低級脂肪族ケトン；１，３−ブチレングリコール、プロピレングリコール、グリセリン等の炭素数２〜５の多価アルコール等が挙げられる。 Examples of hydrophilic organic solvents that can be used as extraction solvents include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.
２種以上の極性溶媒の混合液を抽出溶媒として使用する場合、その混合比は適宜調整することができる。例えば、水と低級脂肪族アルコールとの混合液を抽出溶媒として使用する場合には、水と低級脂肪族アルコールとの混合比が９：１〜１：９（容量比）であることが好ましく、７：３〜２：８（容量比）であることがさらに好ましい。また、水と低級脂肪族ケトンとの混合液を使用する場合には、水と低級脂肪族ケトンとの混合比が９：１〜２：８（容量比）であることが好ましく、水と多価アルコールとの混合液を使用する場合には、水と多価アルコールとの混合比が５：５〜１：９（容量比）であることが好ましい。 When using the liquid mixture of 2 or more types of polar solvents as an extraction solvent, the mixing ratio can be adjusted suitably. For example, when a mixed liquid of water and lower aliphatic alcohol is used as an extraction solvent, the mixing ratio of water and lower aliphatic alcohol is preferably 9: 1 to 1: 9 (volume ratio), More preferably, it is 7: 3 to 2: 8 (capacity ratio). In addition, when a mixed liquid of water and a lower aliphatic ketone is used, the mixing ratio of water and the lower aliphatic ketone is preferably 9: 1 to 2: 8 (volume ratio). When using the liquid mixture with a monohydric alcohol, it is preferable that the mixing ratio of water and a polyhydric alcohol is 5: 5 to 1: 9 (volume ratio).
抽出処理は、抽出原料に含まれる可溶性成分を抽出溶媒に溶出させ得る限り特に限定はされず、常法に従って行うことができる。例えば、抽出原料の５〜１５倍量（質量比）の抽出溶媒に、抽出原料を浸漬し、常温又は還流加熱下で可溶性成分を抽出させた後、濾過して抽出残渣を除去することにより抽出液を得ることができる。得られた抽出液から溶媒を留去するとペースト状の濃縮物が得られ、この濃縮物をさらに乾燥すると乾燥物が得られる。 The extraction treatment is not particularly limited as long as the soluble component contained in the extraction raw material can be eluted in the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in an extraction solvent 5 to 15 times (mass ratio) of the extraction raw material, the soluble components are extracted at room temperature or under reflux, and then filtered to remove the extraction residue. A liquid can be obtained. When the solvent is distilled off from the obtained extract, a paste-like concentrate is obtained, and when this concentrate is further dried, a dried product is obtained.
なお、上述のようにして得られた抽出液はそのままでもメラニン産生抑制剤又はコラーゲン産生促進剤の有効成分として使用することができるが、濃縮液又は乾燥物としたものの方が使用しやすい。 In addition, although the extract obtained as mentioned above can be used as an active ingredient of a melanin production inhibitor or a collagen production promoter as it is, a concentrated solution or a dried product is easier to use.
また、パイナップルからの抽出物やキンモクセイからの抽出物は特有の匂いを有しているため、その生理活性の低下を招かない範囲で脱色、脱臭等を目的とする精製を行うことも可能であるが、皮膚化粧料等に配合する場合には大量に使用するものではないから、未精製のままでも実用上支障はない。 In addition, since the extract from pineapple and the extract from cinnamon have a unique odor, it is possible to perform purification for the purpose of decolorization, deodorization, etc. within a range that does not cause a decrease in the physiological activity. However, since it is not used in a large amount when blended into skin cosmetics, there is no practical problem even if it is not purified.
ここで、本実施形態において用いられるパイナップルからの抽出物は、その構成成分としてグルコシルセラミドを２質量％以上含有することが好ましく、３〜２５質量％含有することがさらに好ましい。かかる含有量でグルコシルセラミドを含有することで、キンモクセイ抽出物との相乗効果がより顕著になり、より優れたメラニン産生抑制作用及びコラーゲン産生促進作用を発揮することができる。 Here, the extract from pineapple used in the present embodiment preferably contains 2% by mass or more, more preferably 3 to 25% by mass, of glucosylceramide as a constituent component. By containing glucosylceramide at such a content, the synergistic effect with the cinnamon extract is more prominent, and more excellent melanin production inhibitory action and collagen production promoting action can be exhibited.
〔メラニン産生抑制剤，コラーゲン産生促進剤〕
以上のようにして得られるパイナップルからの抽出物及びキンモクセイからの抽出物は、それぞれ単独で使用するよりも、これらを併せて使用することで相乗効果を発揮し、優れたメラニン産生抑制作用及びコラーゲン産生促進作用を示す。そのため、パイナップルからの抽出物とキンモクセイからの抽出物とを併用することで、これらをメラニン産生抑制剤及びコラーゲン産生促進剤の有効成分として用いることができる。本実施形態のメラニン産生抑制剤又はコラーゲン産生促進剤は、医薬品、医薬部外品、化粧品等の幅広い用途に使用することができる。
[Melanin production inhibitor, collagen production promoter]
The extract from pineapple and the extract from Buttercup that are obtained as described above exhibit a synergistic effect by using them together rather than using them alone, and have excellent melanin production inhibitory action and collagen Shows production promotion effect. Therefore, the combined use of the extract from pineapple and the extract from cinnamon can be used as active ingredients of a melanin production inhibitor and a collagen production promoter. The melanin production inhibitor or collagen production promoter of this embodiment can be used for a wide range of applications such as pharmaceuticals, quasi drugs, and cosmetics.
なお、本実施形態において、パイナップルからの抽出物とキンモクセイからの抽出物との含有量比は、質量換算で１：１〜１：１００であることが好ましく、１：３〜１：２０であることがさらに好ましい。また、上記パイナップルからの抽出物がグルコシルセラミドを含有する場合、当該パイナップル抽出物に含まれるグルコシルセラミドとキンモクセイからの抽出物との含有量比は、質量換算で１：１〜１：２５０であることが好ましく、１：１５〜１：１００であることがより好ましい。かかる含有量比にてパイナップル抽出物とキンモクセイ抽出物とを含有させることで、その相乗効果がより顕著になり、より優れたメラニン産生抑制作用及びコラーゲン産生促進作用を発揮することができる。 In addition, in this embodiment, it is preferable that the content ratio of the extract from a pineapple and the extract from a cinnamon is 1: 1-1: 100 by mass conversion, and is 1: 3-1: 20. More preferably. Moreover, when the extract from the pineapple contains glucosylceramide, the content ratio of the glucosylceramide contained in the pineapple extract and the extract from cinnamon is 1: 1 to 1: 250 in terms of mass. Preferably, it is 1:15 to 1: 100. By including the pineapple extract and the cinnamon extract at such a content ratio, the synergistic effect becomes more prominent, and a more excellent melanin production suppressing action and collagen production promoting action can be exhibited.
本実施形態のメラニン産生抑制剤又はコラーゲン産生促進剤は、パイナップルからの抽出物及びキンモクセイからの抽出物のみからなるものでもよいし、これらの抽出物を配合・製剤化したものでもよい。 The melanin production inhibitor or collagen production promoter of the present embodiment may be composed only of an extract from pineapple and an extract from cinnamon mushroom, or may be a mixture / formulation of these extracts.
本実施形態のメラニン産生抑制剤又はコラーゲン産生促進剤は、デキストリン、シクロデキストリン等の薬学的に許容し得るキャリアーその他任意の助剤を用いて、常法に従い、粉末状、顆粒状、錠剤状、液状等の任意の剤形に製剤化することができる。この際、助剤としては、例えば、賦形剤、結合剤、崩壊剤、滑沢剤、安定剤、矯味・矯臭剤等を用いることができる。メラニン産生抑制剤又はコラーゲン産生促進剤は、他の組成物（例えば、皮膚外用剤、美容用飲食品等）に配合して使用することができるほか、軟膏剤、外用液剤、貼付剤等として使用することができる。 The melanin production inhibitor or collagen production promoter of this embodiment is a powder, granule, tablet, or the like according to a conventional method using a pharmaceutically acceptable carrier such as dextrin or cyclodextrin or any other auxiliary agent. It can be formulated into any dosage form such as liquid. In this case, as an auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring / flavoring agent, and the like can be used. The melanin production inhibitor or collagen production promoter can be used by blending with other compositions (for example, external preparations for skin, foods and beverages for cosmetics, etc.), and also used as an ointment, a liquid for external use, a patch, etc. can do.
本実施形態のメラニン産生抑制剤又はコラーゲン産生促進剤を製剤化した場合、パイナップルからの抽出物及びキンモクセイからの抽出物の合計含有量は、特に限定されるものではなく、目的に応じて適宜設定することができる。 When the melanin production inhibitor or collagen production promoter of the present embodiment is formulated, the total content of the extract from pineapple and the extract from cinnamon is not particularly limited, and is appropriately set according to the purpose. can do.
なお、本実施形態のメラニン産生抑制剤又はコラーゲン産生促進剤は、必要に応じて、メラニン産生抑制作用又はコラーゲン産生促進作用を有する他の天然抽出物等を、パイナップルからの抽出物及びキンモクセイからの抽出物とともに配合して有効成分として用いることができる。 In addition, the melanin production inhibitor or the collagen production promoter of the present embodiment, if necessary, extracts other natural extracts having a melanin production inhibitory action or a collagen production promotion action from extracts from pineapples and cinnamon moss. It can be used together with the extract as an active ingredient.
本実施形態のメラニン産生抑制剤又はコラーゲン産生促進剤の患者に対する投与方法としては、経皮投与、経口投与等が挙げられるが、疾患の種類に応じて、その予防・治療等に好適な方法を適宜選択すればよい。 Examples of the method of administration of the melanin production inhibitor or collagen production promoter of the present embodiment to a patient include transdermal administration, oral administration, and the like. Depending on the type of disease, a suitable method for its prevention / treatment, etc. What is necessary is just to select suitably.
また、本実施形態のメラニン産生抑制剤又はコラーゲン産生促進剤の投与量も、疾患の種類、重症度、患者の個人差、投与方法、投与期間等によって適宜増減すればよい。 In addition, the dose of the melanin production inhibitor or collagen production promoter of the present embodiment may be appropriately increased or decreased depending on the disease type, severity, patient differences, administration method, administration period, and the like.
本実施形態のメラニン産生抑制剤は、パイナップルからの抽出物及びキンモクセイからの抽出物が有するメラニン産生抑制作用を通じて、シミ、ソバカス等を予防、治療又は改善することができる。また、本実施形態のメラニン産生抑制剤は、メラニンの異常産生に起因する疾患の予防・治療用医薬品又は医薬部外品の有効成分として用いることができる。ただし、本実施形態のメラニン産生抑制剤は、これらの用途以外にもメラニン産生抑制作用を発揮することに意義のあるすべての用途に用いることができる。 The melanin production inhibitor of the present embodiment can prevent, treat, or improve spots, buckwheat, and the like through the melanin production inhibitory action of the extract from pineapple and the extract from betel grass. Moreover, the melanin production inhibitor of this embodiment can be used as an active ingredient of the pharmaceutical for prevention and treatment of the disease resulting from abnormal production of melanin, or a quasi-drug. However, the melanin production inhibitor of the present embodiment can be used for all purposes other than these uses, which are meaningful for exerting a melanin production inhibitory action.
また、本実施形態のコラーゲン産生促進剤は、パイナップルからの抽出物及びキンモクセイからの抽出物が有するＩ型コラーゲン産生促進作用を通じて、しわ、たるみ等の皮膚の老化症状を予防又は改善することができる。また、本実施形態のコラーゲン産生促進剤は、パイナップルからの抽出物及びキンモクセイからの抽出物が有するコラーゲン産生促進作用を通じて、骨粗鬆症等のコラーゲン産生の低下に起因する疾患の予防、治療又は改善；損傷した腱や靱帯の再生促進；創傷又は熱傷の治癒の促進；等の用途に用いることができる。ただし、本実施形態のＩ型コラーゲン産生促進剤は、これらの用途以外にもＩ型コラーゲン産生促進作用を発揮することに意義のあるすべての用途に用いることができる。 Moreover, the collagen production promoter of this embodiment can prevent or ameliorate skin aging symptoms such as wrinkles and sagging through the action of promoting type I collagen production of the extract from pineapple and the extract from cinnamon moss. . In addition, the collagen production promoter of the present embodiment prevents, treats or ameliorates diseases caused by decreased collagen production such as osteoporosis through the collagen production promoting action of the extract from pineapple and the extract from cinnamon moth; It can be used for the purpose of promoting the regeneration of damaged tendons and ligaments; promoting the healing of wounds or burns; However, the type I collagen production promoter of the present embodiment can be used for all purposes that are meaningful for exerting a type I collagen production promoting effect in addition to these uses.
また、本実施形態のメラニン産生抑制剤及びコラーゲン産生促進剤は、優れたメラニン産生抑制作用及びコラーゲン産生促進作用を有するため、例えば、皮膚外用剤に配合するのに好適である。この場合に、パイナップルからの抽出物及びキンモクセイからの抽出物をそれぞれそのまま配合してもよく、またこれらの混合物をそのまま配合してもよく、さらにはパイナップルからの抽出物及びキンモクセイからの抽出物を配合・製剤化したメラニン産生抑制剤又はコラーゲン産生促進剤を配合してもよい。 Moreover, since the melanin production inhibitor and collagen production promoter of this embodiment have the outstanding melanin production inhibitory effect and collagen production promotion effect, they are suitable for mix | blending with a skin external preparation, for example. In this case, the extract from pineapple and the extract from cinnamon can be blended as they are, respectively, these mixtures can be blended as they are, and further, the extract from pineapple and the extract from cinnamon are used. A blended and formulated melanin production inhibitor or collagen production promoter may be blended.
ここで、皮膚外用剤としては、その区分に制限はなく、後述する皮膚化粧料のほか、経皮的に使用される医薬部外品、医薬品等を幅広く含むものである。 Here, the external preparation for skin is not limited in its category, and includes a wide range of quasi-drugs and pharmaceuticals used transdermally, in addition to the skin cosmetics described below.
また、本実施形態のメラニン産生抑制剤及びコラーゲン産生促進剤は、優れたメラニン産生抑制作用及びコラーゲン産生促進作用を有するので、メラニンの産生機構に関連する研究やＩ型コラーゲンの産生機構に関連する研究のための試薬としても好適に利用することができる。 Moreover, since the melanin production inhibitor and collagen production promoter of this embodiment have an excellent melanin production suppression action and collagen production promotion action, they are related to research related to melanin production mechanism and type I collagen production mechanism. It can be suitably used as a reagent for research.
〔皮膚化粧料〕
パイナップルからの抽出物及びキンモクセイからの抽出物は、優れたメラニン産生抑制作用及びコラーゲン産生促進作用を有しているため、皮膚化粧料に配合するのに好適である。この場合、パイナップルからの抽出物及びキンモクセイからの抽出物をそれぞれそのまま配合してもよく、またこれらの混合物をそのまま配合してもよく、さらにはパイナップルからの抽出物及びキンモクセイからの抽出物を配合・製剤化したメラニン産生抑制剤又はコラーゲン産生促進剤を配合してもよい。パイナップルからの抽出物及びキンモクセイからの抽出物、若しくはこれらの混合物、上記メラニン産生抑制剤、又は上記コラーゲン産生促進剤を皮膚化粧料に配合することによって、皮膚化粧料にメラニン産生抑制作用又はコラーゲン産生促進作用を付与することができる。
[Skin cosmetic]
The extract from pineapple and the extract from cinnamon are suitable for blending into skin cosmetics because they have excellent melanin production inhibitory action and collagen production promoting action. In this case, the extract from pineapple and the extract from cinnamon can be blended as they are, respectively, these mixtures can be blended as they are, and further, the extract from pineapple and the extract from cinnamon are combined. -You may mix | blend the formulated melanin production inhibitor or collagen production promoter. By blending an extract from pineapple and an extract from betel grass, or a mixture thereof, the above melanin production inhibitor, or the above collagen production promoter into skin cosmetics, the skin cosmetics can suppress melanin production or produce collagen. A promoting action can be imparted.
パイナップルからの抽出物及びキンモクセイからの抽出物、若しくはこれらの混合物、上記メラニン産生抑制剤、又は上記コラーゲン産生促進剤を配合し得る皮膚化粧料としては、特に限定されるものではなく、例えば、軟膏、クリーム、乳液、ローション、パック、ファンデーション、リップクリーム、入浴剤、ヘアートニック、ヘアーローション、石鹸、ボディシャンプー等が挙げられる。 Skin cosmetics that can be blended with an extract from pineapple and an extract from cinnamon oyster, or a mixture thereof, the melanin production inhibitor, or the collagen production promoter are not particularly limited. , Creams, emulsions, lotions, packs, foundations, lip balms, bath salts, hair nicks, hair lotions, soaps, body shampoos and the like.
パイナップルからの抽出物及びキンモクセイからの抽出物又はこれらの混合物を皮膚化粧料に配合する場合、その配合量は、皮膚化粧料の種類に応じて適宜調整することができるが、パイナップルからの抽出物及びキンモクセイからの抽出物の合計量で換算した場合の好適な配合率は、約０．０００１〜１０質量％であり、特に好適な配合率は約０．００１〜１質量％である。 When an extract from pineapple and an extract from cinnamon or a mixture thereof are blended in skin cosmetics, the blending amount can be adjusted as appropriate according to the type of skin cosmetic, but an extract from pineapples And the suitable compounding rate at the time of converting with the total amount of the extract from a cinnamon is about 0.0001-10 mass%, and a particularly suitable compounding rate is about 0.001-1 mass%.
本実施形態の皮膚化粧料は、パイナップルからの抽出物及びキンモクセイからの抽出物が有するメラニン産生抑制作用又はコラーゲン産生促進作用を妨げない限り、通常の皮膚化粧料の製造に用いられる主剤、助剤又はその他の成分、例えば、収斂剤、殺菌・抗菌剤、美白剤、紫外線吸収剤、保湿剤、細胞賦活剤、消炎・抗アレルギー剤、抗酸化・活性酸素除去剤、油脂類、ロウ類、炭化水素類、脂肪酸類、アルコール類、エステル類、界面活性剤、香料等を併用することができる。このように併用することで、より一般性のある製品となり、また、併用された他の有効成分との間の相乗作用が通常期待される以上の優れた効果をもたらすことがある。 The skin cosmetic of the present embodiment is a main agent or auxiliary used in the production of normal skin cosmetics, as long as it does not interfere with the melanin production inhibiting action or collagen production promoting action of the extract from pineapple and the extract from quince Or other ingredients such as astringents, bactericides / antibacterial agents, whitening agents, UV absorbers, moisturizers, cell activators, anti-inflammatory / anti-allergic agents, antioxidant / active oxygen removers, fats and oils, waxes, carbonization Hydrogens, fatty acids, alcohols, esters, surfactants, fragrances and the like can be used in combination. When used in combination, it becomes a more general product, and a synergistic effect with other active ingredients used in combination may lead to an excellent effect that is more than normally expected.
本実施形態の皮膚化粧料は、メラニン産生抑制作用又はコラーゲン産生促進作用を通じて、シミ、ソバカス等の予防、治療又は改善；メラニンの異常産生に起因する疾患の予防・治療；しわ、たるみ等の皮膚の老化症状の予防又は改善；骨粗鬆症等のコラーゲン産生の低下に起因する疾患の予防、治療又は改善；損傷した腱や靱帯の再生促進；創傷又は熱傷の治癒の促進；等の用途に用いることができる。 The skin cosmetic according to the present embodiment prevents, treats or improves spots, buckwheat, etc., through melanin production inhibiting action or collagen production promoting action; prevention / treatment of diseases caused by abnormal production of melanin; wrinkles, sagging skin, etc. To prevent or improve the aging symptoms of patients; prevention, treatment or improvement of diseases caused by decreased collagen production such as osteoporosis; promotion of regeneration of damaged tendons and ligaments; promotion of healing of wounds or burns; it can.
〔飲食品〕
パイナップルからの抽出物及びキンモクセイからの抽出物は、優れたメラニン産生抑制作用及びコラーゲン産生促進作用を有しているため、飲食品に配合するのに好適である。この場合、この場合、パイナップルからの抽出物及びキンモクセイからの抽出物をそれぞれそのまま配合してもよく、またこれらの混合物をそのまま配合してもよく、さらにはパイナップルからの抽出物及びキンモクセイからの抽出物を配合・製剤化したメラニン産生抑制剤又はコラーゲン産生促進剤を配合してもよい。
[Food and Drink]
Since the extract from pineapple and the extract from Buttercup have excellent melanin production inhibitory action and collagen production promoting action, they are suitable for blending into foods and drinks. In this case, in this case, the extract from the pineapple and the extract from the buttercup may be blended as they are, respectively, or the mixture thereof may be blended as it is, and further the extract from the pineapple and the extract from the buttercup A melanin production inhibitor or collagen production promoter containing a product may be blended.
ここで、飲食品とは、人の健康に危害を加えるおそれが少なく、通常の社会生活において、経口又は消化管投与により摂取されるものをいい、行政区分上の食品、医薬品、医薬部外品等の区分に制限されるものではない。したがって、本実施形態における「飲食品」は、経口的に摂取される一般食品、健康食品、保健機能食品、医薬部外品、医薬品等を幅広く含むものである。 Here, food and drink are those that are less likely to harm human health and are taken by oral or gastrointestinal administration in normal social life. It is not limited to such categories. Therefore, the “food or drink” in this embodiment includes a wide variety of general foods, health foods, health functional foods, quasi drugs, pharmaceuticals, and the like that are taken orally.
パイナップルからの抽出物及びキンモクセイからの抽出物、若しくはこれらの混合物、又はパイナップルからの抽出物及びキンモクセイからの抽出物を配合・製剤化したメラニン産生抑制剤若しくはコラーゲン産生促進剤を飲食品に配合する場合、それらにおける有効成分の配合量は、使用目的、症状、性別等を考慮して適宜変更することができるが、添加対象となる飲食品の一般的な摂取量を考慮して、成人１日あたりの抽出物摂取量が、パイナップルからの抽出物及びキンモクセイからの抽出物の合計で約１〜１０００ｍｇになるようにするのが好ましい。なお、添加対象飲食品が顆粒状、錠剤状又はカプセル状の飲食品の場合、メラニン産生抑制剤又はコラーゲン産生促進剤の添加量は、添加対象飲食品に対して通常０．１〜１００質量％であり、好ましくは５〜１００質量％である。 A melanin production inhibitor or a collagen production promoter that is formulated and formulated with an extract from pineapple and an extract from betel grass, or a mixture thereof, or an extract from pineapple and an extract from betel grass is added to food and drink. In this case, the amount of the active ingredient in them can be appropriately changed in consideration of the purpose of use, symptoms, sex, etc., but in consideration of the general intake of foods and drinks to be added, the adult day It is preferable that the amount of the perilla extract intake is about 1 to 1000 mg in total of the extract from pineapple and the extract from cinnamon. In addition, when the food and drink to be added is a granular, tablet or capsule food or drink, the addition amount of the melanin production inhibitor or the collagen production promoter is usually 0.1 to 100% by mass with respect to the food and drink to be added. Preferably, it is 5-100 mass%.
本実施形態の飲食品は、パイナップルからの抽出物及びキンモクセイからの抽出物又はこれらの混合物を、その活性を妨げないような任意の飲食品に配合したものであってもよいし、パイナップルからの抽出物及びキンモクセイからの抽出物又はこれらの混合物を主成分とする栄養補助食品であってもよい。 The food / beverage products of this embodiment may be an extract from pineapple, an extract from cinnamon moss or a mixture thereof in any food / beverage product that does not impede its activity, or from pineapple. It may also be a dietary supplement based on an extract and an extract from a Snapper or a mixture thereof.
本実施形態の飲食品を製造する際には、例えば、デキストリン、デンプン等の糖類；ゼラチン、大豆タンパク、トウモロコシタンパク等のタンパク質；アラニン、グルタミン、イソロイシン等のアミノ酸類；セルロース、アラビアゴム等の多糖類；大豆油、中鎖脂肪酸トリグリセリド等の油脂類などの任意の助剤を添加して任意の形状の飲食品にすることができる。 When producing the food and drink of this embodiment, for example, sugars such as dextrin and starch; proteins such as gelatin, soybean protein and corn protein; amino acids such as alanine, glutamine and isoleucine; Arbitrary auxiliary agents, such as saccharides; oils and fats, such as soybean oil and medium chain fatty acid triglyceride, can be added, and it can be set as food / beverage products of arbitrary shapes.
パイナップルからの抽出物及びキンモクセイからの抽出物又はこれらの混合物を配合し得る飲食品は特に限定されないが、その具体例としては、清涼飲料、炭酸飲料、栄養飲料、果実飲料、乳酸飲料等の飲料（これらの飲料の濃縮原液及び調整用粉末を含む）；アイスクリーム、アイスシャーベット、かき氷等の冷菓；そば、うどん、はるさめ、ぎょうざの皮、しゅうまいの皮、中華麺、即席麺等の麺類；飴、チューインガム、キャンディー、ガム、チョコレート、錠菓、スナック菓子、ビスケット、ゼリー、ジャム、クリーム、焼き菓子等の菓子類；かまぼこ、ハム、ソーセージ等の水産・畜産加工食品；加工乳、発酵乳等の乳製品；サラダ油、てんぷら油、マーガリン、マヨネーズ、ショートニング、ホイップクリーム、ドレッシング等の油脂及び油脂加工食品；ソース、たれ等の調味料；スープ、シチュー、サラダ、惣菜、漬物；その他種々の形態の健康・栄養補助食品；錠剤、カプセル剤、ドリンク剤などが挙げられ、これらの飲食品にパイナップルからの抽出物及びキンモクセイからの抽出物又はこれらの混合物を配合するときに、通常用いられる補助的な原料や添加物を併用することができる。 Foods and drinks that can be blended with an extract from pineapple and an extract from cinnamon or a mixture thereof are not particularly limited, and specific examples thereof include beverages such as soft drinks, carbonated drinks, nutrition drinks, fruit drinks, and lactic acid drinks. (Contains concentrated beverage concentrate and powder for adjustment); Ice cream, ice sherbet, shaved ice, and other frozen desserts; buckwheat noodles, udon, harsame, gyoza skin, sukimai skin, Chinese noodles, instant noodles and other noodles; , Chewing gum, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, etc .; fishery and livestock processed foods such as kamaboko, ham, sausage; milk such as processed milk, fermented milk Products: salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing, etc. Fats and oils and processed foods; sauces, sauces and other seasonings; soups, stews, salads, prepared dishes, pickles; various other forms of health and nutritional supplements; tablets, capsules, drinks, etc. When blending an extract from pineapple and an extract from cinnamon moss or a mixture thereof into a product, auxiliary materials and additives that are usually used can be used in combination.
なお、本実施形態のメラニン産生抑制剤、コラーゲン産生促進剤、皮膚化粧料及び飲食品は、ヒトに対して好適に適用されるものであるが、それぞれの作用効果が奏される限り、ヒト以外の動物（例えば，マウス，ラット，ハムスター，イヌ，ネコ，ウシ，ブタ，サル等）に対して適用することもできる。 In addition, although the melanin production inhibitor, collagen production promoter, skin cosmetics, and foods and beverages of this embodiment are suitably applied to humans, as long as the respective effects are exhibited, other than humans The present invention can also be applied to other animals (eg, mouse, rat, hamster, dog, cat, cow, pig, monkey, etc.).
以下、試験例を示し、本発明を具体的に説明するが、本発明は下記の各例に何ら制限されるものではない。なお、下記試験例においては、パイナップル抽出物としてパイナップル果実抽出物Ａ（丸善製薬社製，セラミド２０％含有，試料１）又はパイナップル果実抽出物Ｂ（丸善製薬社製，セラミド４％含有，試料２）を、またキンモクセイからの抽出物として桂花抽出物（丸善製薬社製，試料３）を、それぞれ使用した。 Hereinafter, although a test example is shown and this invention is demonstrated concretely, this invention is not restrict | limited to each following example at all. In the following test examples, pineapple fruit extract A (manufactured by Maruzen Pharmaceutical Co., Ltd., containing 20% ceramide, sample 1) or pineapple fruit extract B (manufactured by Maruzen Pharmaceutical Co., Ltd., containing 4% ceramide, sample 2) ), And Katsura flower extract (manufactured by Maruzen Pharmaceutical Co., Ltd., sample 3) was used as an extract from the cinnamon ox.
〔試験例１〕Ｂ１６メラノーマ細胞に対するメラニン産生抑制作用試験
上記抽出物（試料１及び３）について、以下のようにしてＢ１６メラノーマ細胞に対するメラニン産生抑制作用を試験した。
[Test Example 1] Test for inhibiting melanin production on B16 melanoma cells The extract (samples 1 and 3) was tested for melanin production inhibiting action on B16 melanoma cells as follows.
Ｂ１６メラノーマ細胞を、１０％ＦＢＳ含有ダルベッコＭＥＭ培地を用いて培養した後、トリプシン処理により細胞を回収した。回収した細胞を２４．０×１０４ｃｅｌｌｓ／ｍＬの細胞密度になるように１０％ＦＢＳ及び１ｍｍｏｌ／Ｌテオフィリン含有ダルベッコＭＥＭ培地で希釈した後、４８ウェルプレートに１ウェルあたり３００μＬずつ播種し、６時間培養した。 B16 melanoma cells were cultured using Dulbecco's MEM medium containing 10% FBS, and then cells were collected by trypsin treatment. The collected cells were diluted with Dulbecco's MEM medium containing 10% FBS and 1 mmol / L theophylline to a cell density of 24.0 × 10 4 cells / mL, and then seeded at 300 μL per well in a 48-well plate. Incubate for hours.
培養終了後、被験試料（試料１及び／又は試料３，試料濃度は下記表１を参照）を添加した１０％ＦＢＳ及び１ｍｍｏｌ／Ｌテオフィリン含有ダルベッコＭＥＭ培地又は試料無添加の１０％ＦＢＳ及び１ｍｍｏｌ／Ｌテオフィリン含有ダルベッコＭＥＭ培地を各ウェルに３００μＬ添加し、４日間培養した。培養終了後、各ウェルから培地を取り除き、２ｍｏｌ／ＬのＮａＯＨ溶液２００μＬを添加して超音波破砕機により細胞を破壊し、波長４７５ｎｍにおける吸光度を測定してメラニン産生量とした。 After completion of the culture, 10% FBS and 1 mmol / L theophylline-containing Dulbecco's MEM medium to which a test sample (sample 1 and / or sample 3, sample concentration is shown below) is added, or 10% FBS and 1 mmol / L without the sample are added. 300 μL of L theophylline-containing Dulbecco's MEM medium was added to each well and cultured for 4 days. After completion of the culture, the medium was removed from each well, 200 μL of 2 mol / L NaOH solution was added, the cells were disrupted with an ultrasonic disrupter, and the absorbance at a wavelength of 475 nm was measured to obtain the amount of melanin produced.
また、細胞生存率を測定するために、上記と同様にして培養した後、培地を除去し４００μＬのＰＢＳ緩衝液で洗浄して、終濃度０．０５ｍｇ／ｍＬで１０％ＦＢＳ含有ダルベッコＭＥＭに溶解したニュートラルレッドを各ウェルに２００μＬ添加し、２．５時間培養した。培養後、ニュートラルレッド溶液を除去し、エタノール・酢酸溶液（エタノール：酢酸：水＝５０：１：４９）を各ウェルに２００μＬ添加し、色素を抽出した。抽出後、波長５４０ｎｍにおける吸光度を測定した。得られた結果から、下記式により細胞生存率により補正したメラニン産生抑制率（％）を算出した。 In order to measure cell viability, the cells were cultured in the same manner as described above, then the medium was removed, washed with 400 μL of PBS buffer, and dissolved in Dulbecco MEM containing 10% FBS at a final concentration of 0.05 mg / mL. 200 μL of the neutral red was added to each well and cultured for 2.5 hours. After incubation, the neutral red solution was removed, and 200 μL of ethanol / acetic acid solution (ethanol: acetic acid: water = 50: 1: 49) was added to each well to extract the dye. After extraction, the absorbance at a wavelength of 540 nm was measured. From the obtained results, the melanin production inhibition rate (%) corrected by the cell viability was calculated by the following formula.
メラニン産生抑制率(％)＝{１−（Ｂ／Ｄ）／（Ａ／Ｃ）}×１００
上記式において、Ａは「試料無添加での４７５ｎｍにおける吸光度」を表し、Ｂは「被験試料添加での４７５ｎｍにおける吸光度」を表し、Ｃは「試料無添加での５４０ｎｍにおける吸光度」を表し、Ｄは「被験試料添加での５４０ｎｍにおける吸光度」を表す。
結果を表１に示す。
Melanin production inhibition rate (%) = {1− (B / D) / (A / C)} × 100
In the above formula, A represents “absorbance at 475 nm without addition of sample”, B represents “absorbance at 475 nm without addition of test sample”, C represents “absorbance at 540 nm without addition of sample”, D Represents “absorbance at 540 nm with addition of test sample”.
The results are shown in Table 1.
表１に示すように、試料１又は試料３は、いずれも単独でメラニンの産生を抑制することができるが、試料１と試料３とを併せて処理することで、相乗的にメラニンの産生を抑制した。したがって、パイナップルからの抽出物とキンモクセイからの抽出物とを併用することで、非常に優れたメラニン産生抑制作用が発揮されるものと認められた。 As shown in Table 1, either sample 1 or sample 3 can independently suppress the production of melanin, but by processing sample 1 and sample 3 together, synergistic production of melanin is achieved. Suppressed. Therefore, it was recognized that a very excellent melanin production inhibitory effect was exhibited by using an extract from pineapple and an extract from Buttercup.
〔試験例２〕Ｉ型コラーゲン産生促進作用試験
上記抽出物（試料２及び３）について、以下のようにしてＩ型コラーゲン産生促進作用を試験した。
[Test Example 2] Type I collagen production promoting action test The extract (samples 2 and 3) was tested for type I collagen production promoting action as follows.
ヒト正常線維芽細胞（ＮＢ１ＲＧＢ）を、１０％ＦＢＳ含有ダルベッコＭＥＭ培地を用いて培養した後、トリプシン処理により細胞を回収した。回収した細胞を１．６×１０５ｃｅｌｌｓ／ｍＬの細胞密度になるように上記培地で希釈した後、９６ウェルマイクロプレートに１ウェルあたり１００μＬずつ播種し、一晩培養した。 Human normal fibroblasts (NB1RGB) were cultured using Dulbecco's MEM medium containing 10% FBS, and then cells were collected by trypsin treatment. The collected cells were diluted with the above medium to a cell density of 1.6 × 10 5 cells / mL, then seeded at 100 μL per well in a 96-well microplate and cultured overnight.
培養終了後、培地を抜き、被験試料（試料２及び／又は試料３，試料濃度は下記表２を参照）を添加した０．２５％ＦＢＳ含有ダルベッコＭＥＭ培地又は試料無添加の０．２５％ＦＢＳ含有ダルベッコＭＥＭ培地を各ウェルに１５０μＬずつ添加し、３日間培養した。培養後、各ウェルの培地中のＩ型コラーゲン量をＥＬＩＳＡ法により測定した。測定結果から、下記式によりＩ型コラーゲン産生促進率（％）を算出した。 After completion of the culture, the medium was removed and Dulbecco's MEM medium containing 0.25% FBS to which the test sample (sample 2 and / or sample 3, sample concentration is shown in Table 2 below) was added or 0.25% FBS without the sample added. 150 μL of Dulbecco's MEM medium was added to each well and cultured for 3 days. After culture, the amount of type I collagen in the medium of each well was measured by ELISA. From the measurement results, the type I collagen production promotion rate (%) was calculated by the following formula.
Ｉ型コラーゲン産生促進率(％)＝Ａ／Ｂ×１００
上記式において、Ａは「被験試料添加でのＩ型コラーゲン量」を表し、Ｂは「試料無添加でのＩ型コラーゲン量」を表す。
結果を表２に示す。
Type I collagen production promotion rate (%) = A / B × 100
In the above formula, A represents “amount of type I collagen with addition of test sample”, and B represents “amount of type I collagen without addition of sample”.
The results are shown in Table 2.
表２に示すように、試料２又は試料３は、いずれも単独ではＩ型コラーゲンの産生を促進することができないにもかかわらず、試料２と試料３とを併せて処理することで、Ｉ型コラーゲンの産生を促進した。したがって、パイナップルからの抽出物とキンモクセイからの抽出物とを併用することで、非常に優れたＩ型コラーゲン産生促進作用が発揮されるものと認められた。 As shown in Table 2, both Sample 2 and Sample 3 were treated together with Sample 2 and Sample 3 even though they were unable to promote type I collagen production alone. Promoted collagen production. Therefore, it was recognized that a combination of an extract from pineapple and an extract from cinnamon moss exhibited a very excellent type I collagen production promoting effect.
〔配合例１〕
下記組成に従い、乳液を常法により製造した。
パイナップル果実抽出物Ａ ０．２５ｇ
桂花抽出物 ２．０ｇ
ホホバオイル ４．０ｇ
１，３−ブチレングリコール ３．０ｇ
アルブチン ３．０ｇ
ポリオキシエチレンセチルエーテル（20E.O.） ２．５ｇ
オリーブオイル ２．０ｇ
スクワラン ２．０ｇ
セタノール ２．０ｇ
モノステアリン酸グリセリル ２．０ｇ
オレイン酸ポリオキシエチレンソルビタン（20E.O.） ２．０ｇ
パラオキシ安息香酸メチル ０．１５ｇ
グリチルリチン酸ステアリル ０．１０ｇ
黄杞エキス ０．１０ｇ
グリチルリチン酸ジカリウム ０．１０ｇ
イチョウ葉エキス ０．１０ｇ
コンキオリン ０．１０ｇ
オウバクエキス ０．１０ｇ
カミツレエキス ０．１０ｇ
香料 ０．０５ｇ
精製水 残部（全量を１００ｇとする）
[Formulation Example 1]
A milky lotion was produced by a conventional method according to the following composition.
Pineapple fruit extract A 0.25g
Katsura flower extract 2.0g
Jojoba oil 4.0g
1,3-butylene glycol 3.0 g
Arbutin 3.0g
Polyoxyethylene cetyl ether (20E.O.) 2.5g
Olive oil 2.0g
Squalane 2.0g
Cetanol 2.0g
Glyceryl monostearate 2.0g
Oleic acid polyoxyethylene sorbitan (20E.O.) 2.0g
Methyl paraoxybenzoate 0.15g
Stearyl glycyrrhizinate 0.10g
Twilight extract 0.10g
Dipotassium glycyrrhizinate 0.10g
Ginkgo biloba extract 0.10g
Conchiolin 0.10g
Oat extract 0.10g
Chamomile extract 0.10g
Fragrance 0.05g
Purified water remainder (total amount is 100 g)
〔配合例２〕
下記組成のクリームを常法により製造した。
パイナップル果実抽出物Ｂ ０．３ｇ
桂花抽出物 １．０ｇ
クジンエキス ０．１ｇ
オウゴンエキス ０．１ｇ
流動パラフィン ５．０ｇ
サラシミツロウ ４．０ｇ
スクワラン １０．０ｇ
セタノール ３．０ｇ
ラノリン ２．０ｇ
ステアリン酸 １．０ｇ
オレイン酸ポリオキシエチレンソルビタン（20E.O.） １．５ｇ
モノステアリン酸グリセリル ３．０ｇ
油溶性甘草エキス ０．１ｇ
１，３−ブチレングリコール ６．０ｇ
パラオキシ安息香酸メチル １．５ｇ
香料 ０．１ｇ
精製水 残部（全量を１００ｇとする）
[Formulation Example 2]
A cream having the following composition was produced by a conventional method.
Pineapple fruit extract B 0.3g
Katsura Flower Extract 1.0g
Kujin extract 0.1g
Ogon Extract 0.1g
Liquid paraffin 5.0g
Salami beeswax 4.0g
Squalane 10.0g
Cetanol 3.0g
Lanolin 2.0g
Stearic acid 1.0g
Oleic acid polyoxyethylene sorbitan (20E.O.) 1.5g
3.0 g glyceryl monostearate
Oil soluble licorice extract 0.1g
1,3-butylene glycol 6.0 g
1.5 g of methyl paraoxybenzoate
Fragrance 0.1g
Purified water remainder (total amount is 100 g)
〔配合例３〕
下記組成の美容液を常法により製造した。
パイナップル果実抽出物Ａ ０．１ｇ
桂花抽出物 ２．０ｇ
カミツレエキス ０．１ｇ
ニンジンエキス ０．１ｇ
キサンタンガム ０．３ｇ
ヒドロキシエチルセルロース ０．１ｇ
カルボキシビニルポリマー ０．１ｇ
１，３−ブチレングリコール ４．０ｇ
グリチルリチン酸ジカリウム ０．１ｇ
グリセリン ２．０ｇ
水酸化カリウム ０．２５ｇ
香料 ０．０１ｇ
防腐剤（パラオキシ安息香酸メチル） ０．１５ｇ
エタノール ２．０ｇ
精製水 残部（全量を１００ｇとする）
[Composition Example 3]
A serum having the following composition was produced by a conventional method.
Pineapple fruit extract A 0.1g
Katsura flower extract 2.0g
Chamomile extract 0.1g
Carrot extract 0.1g
Xanthan gum 0.3g
Hydroxyethylcellulose 0.1g
Carboxyvinyl polymer 0.1g
1,3-butylene glycol 4.0 g
0.1g dipotassium glycyrrhizinate
Glycerin 2.0g
Potassium hydroxide 0.25g
Fragrance 0.01g
Preservative (Methyl paraoxybenzoate) 0.15g
Ethanol 2.0g
Purified water remainder (total amount is 100 g)
〔配合例４〕
常法により、以下の組成を有する錠剤を製造した。
パイナップル果実抽出物Ａ ３．０ｍｇ
桂花抽出物 １２．５ｍｇ
ドロマイト（カルシウム２０％、マグネシウム１０％含有） ７８．４ｍｇ
カゼインホスホペプチド １６．７ｍｇ
ビタミンＣ ３３．４ｍｇ
マルチトール １３１．３ｍｇ
コラーゲン １２．７ｍｇ
ショ糖脂肪酸エステル １２．０ｍｇ
[Formulation Example 4]
A tablet having the following composition was produced by a conventional method.
Pineapple fruit extract A 3.0mg
Katsura flower extract 12.5mg
Dolomite (containing 20% calcium and 10% magnesium) 78.4mg
Casein phosphopeptide 16.7mg
Vitamin C 33.4mg
Maltitol 131.3mg
Collagen 12.7mg
Sucrose fatty acid ester 12.0mg
〔配合例５〕
常法により、以下の組成を有する経口液状製剤を製造した。
＜１アンプル（１本１００ｍＬ）中の組成＞
パイナップル果実抽出物Ａ ０．５質量％
桂花抽出物 ２．０質量％
ソルビット １２．０質量％
安息香酸ナトリウム ０．１質量％
香料 １．０質量％
硫酸カルシウム ０．５質量％
精製水 残部（１００質量％）
[Formulation Example 5]
By an ordinary method, an oral liquid preparation having the following composition was produced.
<Composition in one ampoule (one 100 mL)>
Pineapple fruit extract A 0.5% by mass
Katsura Flower Extract 2.0% by mass
Sorbit 12.0% by mass
Sodium benzoate 0.1% by mass
Fragrance 1.0% by mass
Calcium sulfate 0.5% by mass
Purified water balance (100% by mass)
本発明のメラニン産生抑制剤は、皮膚の色素沈着等の予防・改善に大きく貢献できる。また、本発明のコラーゲン産生促進剤は、しわ、たるみ等の皮膚の老化の予防又は改善に大きく貢献できる。 The melanin production inhibitor of the present invention can greatly contribute to the prevention and improvement of skin pigmentation and the like. Moreover, the collagen production promoter of the present invention can greatly contribute to prevention or improvement of skin aging such as wrinkles and sagging.
Claims (4)
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| CN106924141A (en) * | 2017-03-28 | 2017-07-07 | 海南师范大学 | A kind of preparation method of the pineapple leaves extract for being applied to whitening field |
| WO2020175700A1 (en) * | 2019-02-28 | 2020-09-03 | 株式会社日本触媒 | Aqueous dispersion for cosmetic preparations, cosmetic composition and method for producing cosmetic preparation |
| WO2021256544A1 (en) * | 2020-06-19 | 2021-12-23 | 日本ハルマ株式会社 | Method for extracting ceramide-containing material or ceramide from whole apples and/or apple juice extraction residue, and composition including said ceramide |
| CN115137678A (en) * | 2022-05-24 | 2022-10-04 | 桂林拓普香料有限公司 | Pure natural sweet osmanthus moistening paste and preparation method thereof |
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| CN106924141A (en) * | 2017-03-28 | 2017-07-07 | 海南师范大学 | A kind of preparation method of the pineapple leaves extract for being applied to whitening field |
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| JPWO2020175700A1 (en) * | 2019-02-28 | 2021-10-07 | 株式会社日本触媒 | Aqueous dispersion for cosmetics, cosmetic composition, and method for producing cosmetics |
| WO2021256544A1 (en) * | 2020-06-19 | 2021-12-23 | 日本ハルマ株式会社 | Method for extracting ceramide-containing material or ceramide from whole apples and/or apple juice extraction residue, and composition including said ceramide |
| CN115137678A (en) * | 2022-05-24 | 2022-10-04 | 桂林拓普香料有限公司 | Pure natural sweet osmanthus moistening paste and preparation method thereof |
| CN115137678B (en) * | 2022-05-24 | 2023-10-24 | 桂林拓普香料有限公司 | Pure natural osmanthus flower moisturizing cream and preparation method thereof |
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