JP6629036B2 - Skin cosmetics and foods and drinks - Google Patents

Description

  TECHNICAL FIELD The present invention relates to a whitening agent, skin cosmetic, and food and drink.

  In skin, melanin also serves to protect the body from ultraviolet radiation, but overproduction and uneven accumulation can cause skin darkening and spots. The melanin granules made of pigment cells (melanocytes) are passed on to the keratinocytes, which migrate to the surface of the skin with the keratinization of the epidermal keratinocytes and are excreted as dirt. You.

  It is known that the proliferation of epidermal keratinocytes is reduced by various causes. For example, it has been reported that the proliferation of epidermal keratinocytes incorporating melanin granules is significantly reduced (see Patent Document 1). When the proliferation of skin keratinocytes is reduced, the proper turnover is impaired, and the melanin granules incorporated in the epidermal keratinocytes are not excreted outside the body and continue to accumulate in the skin. It is believed that this is one of the causes of skin spots.

  Therefore, in order to prevent, treat or ameliorate dark skin (pigmentation skin pigmentation), spots, freckles, etc., it is necessary to not only promote the proliferation of the whole epidermal keratinocytes and the accompanying turnover but also melanin. It is considered to be very effective to specifically promote the proliferation and turnover of epidermal keratinocytes that have taken up granules.

  For example, tranexamic acid or a derivative thereof is known as a component having an epidermal keratinocyte proliferation promoting action and promoting skin turnover (see Patent Document 2).

International Publication No. 2009/113446 JP 2015-34155 A

  The present invention finds a component having a whitening effect among natural-derived components excellent in safety, and provides a whitening agent containing the same as an active ingredient, as well as a skin cosmetic and a food or beverage containing the natural-derived component. The purpose is to do.

  In order to solve the above problems, the whitening agent of the present invention is characterized by containing a lavender sage extract as an active ingredient. Further, the skin cosmetics and foods and drinks of the present invention are characterized by incorporating a lavender sage extract.

  ADVANTAGE OF THE INVENTION According to this invention, by containing a lavender sage extract as an active ingredient, it is possible to provide a whitening agent which is excellent in the effect and high in safety. Furthermore, by blending the lavender sage extract, skin cosmetics and foods and drinks excellent in the above-mentioned effects can be provided.

Hereinafter, embodiments of the present invention will be described.
The whitening agent of the present embodiment contains a lavender sage extract as an active ingredient. In addition, the skin cosmetics and foods and beverages of the present embodiment contain a lavender sage extract.

  Here, the `` lavender sage extract '' in the present embodiment includes an extract obtained by using lavender sage as an extraction raw material, a dilute solution or a concentrated solution of the extract, a dried product obtained by drying the extract, or All of these crude products or purified products are included.

(Whitening agent)
The extraction raw material used in the whitening agent of the present embodiment is lavender sage (scientific name: Salvia 'Indigo Spires').

  Lavender sage (Salvia 'Indigo Spires') is a horticultural variety belonging to the Labiatae family, and can be easily obtained. The constituent parts of the lavender sage that can be used as an extraction raw material include, for example, leaves, stems, roots, flowers, whole plants, and mixtures thereof, and preferably flowers.

  Here, the term "flower" generally refers to the entirety of the organs involved in sexual reproduction of a seed plant, and is composed of a flower leaf that is a deformation of a leaf and a flower axis that is a deformation of a stem. Organs such as calyx, petals, stamens, and carpels are included. The “flora” used as an extraction raw material in the present invention includes, in addition to the whole of the organs involved in sexual reproduction of a seed plant, a part thereof, for example, leaves, florets (seals and corolla), corolla, petals, etc. Is also included.

  The extract from the lavender sage can be obtained by drying the extraction raw material, and then pulverizing it as it is or using a crusher and subjecting it to extraction with an extraction solvent. Drying may be performed in the sun or using a commonly used dryer. Alternatively, the material may be subjected to a pretreatment such as degreasing with a nonpolar solvent such as hexane, and then used as an extraction raw material. By performing a pretreatment such as degreasing, an extraction treatment with a polar solvent can be efficiently performed.

  As the extraction solvent, it is preferable to use a polar solvent, for example, water, a hydrophilic organic solvent and the like. These may be used alone or in combination of two or more, and used at room temperature or a temperature lower than the boiling point of the solvent. Is preferred.

  Examples of water that can be used as the extraction solvent include pure water, tap water, well water, spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those subjected to various treatments. The treatment applied to water includes, for example, purification, heating, sterilization, filtration, ion exchange, osmotic pressure adjustment, buffering, and the like. Therefore, the water that can be used as the extraction solvent in the present embodiment includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

  Examples of the hydrophilic organic solvent that can be used as the extraction solvent include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol, and glycerin.

  When a mixture of two or more polar solvents is used as the extraction solvent, the mixing ratio can be appropriately adjusted. For example, when a mixed solution of water and a lower aliphatic alcohol is used as an extraction solvent, the mixing ratio of water and the lower aliphatic alcohol is preferably 9: 1 to 1: 9 (volume ratio), The ratio is more preferably from 8: 2 to 2: 8 (volume ratio), and particularly preferably from 7: 3 to 3: 7 (volume ratio). When a mixed solution of water and lower aliphatic ketone is used, the mixing ratio of water and lower aliphatic ketone is preferably 9: 1 to 2: 8 (volume ratio), When a mixed solution with a polyhydric alcohol is used, the mixing ratio between water and the polyhydric alcohol is preferably 5: 5 to 1: 9 (volume ratio).

  The extraction treatment is not particularly limited as long as the soluble component contained in the extraction raw material can be eluted into the extraction solvent, and can be performed according to a conventional method. For example, the extraction raw material is immersed in 5 to 15 times (mass ratio) the extraction raw material of the extraction raw material, the soluble component is extracted at room temperature or under reflux, and then filtered to remove the extraction residue. A liquid can be obtained. When the solvent is distilled off from the obtained extract, a paste-like concentrate is obtained, and when this concentrate is further dried, a dry product is obtained.

  Since the lavender sage extract obtained as described above has an excellent whitening effect, it can be used as an active ingredient of a whitening agent. The whitening agent of the present embodiment can be used for a wide range of applications such as pharmaceuticals, quasi-drugs, and cosmetics.

  The whitening effect of the lavender sage extract is exerted on the basis of, for example, an epidermal keratinocyte proliferation promoting effect. However, the whitening action of the lavender sage extract is not limited to the whitening action exerted based on the above action. In addition, the lavender sage extract may be used as an active ingredient of an epidermal keratinocyte proliferation promoter by utilizing its keratinocyte proliferation promoting action.

  The whitening agent of the present embodiment may be composed of only a lavender sage extract, or may be a formulation of the lavender sage extract.

  The whitening agent of the present embodiment is prepared by using a pharmaceutically acceptable carrier such as dextrin and cyclodextrin and other auxiliaries according to a conventional method, and is in the form of powder, granule, tablet, or liquid. Can be formulated. At this time, as an auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavor / odorant, and the like can be used. The whitening agent can be used by being blended with another composition (for example, skin cosmetics and the like), and can be used as an ointment, a liquid for external use, a patch and the like.

  When the whitening agent of the present embodiment is formulated, the content of the lavender sage extract is not particularly limited, and can be appropriately set according to the purpose.

  The whitening agent of the present embodiment can be used as an active ingredient, if necessary, by blending another natural extract having a whitening effect with a lavender sage extract.

  Examples of the method for administering the whitening agent of the present embodiment to a patient include transdermal administration and oral administration, and a method suitable for prevention and treatment of the disease may be appropriately selected according to the type of disease. Further, the dose of the whitening agent of the present embodiment may be appropriately increased or decreased depending on the type and severity of the disease, individual differences among patients, administration methods, administration periods, and the like.

  The whitening agent of the present embodiment promotes the turnover of the skin through the epidermal keratinocyte proliferation promoting action of the lavender sage extract as an active ingredient, blackening of the skin, spots, and prevention of pigmentation such as freckles, Can be treated or improved. Here, where epidermal keratinocytes take up melanin and their proliferation is significantly reduced, the lavender sage extract which is the active ingredient of the present embodiment is, as shown in Examples described later, melanin-incorporated epidermal keratinization. Cell growth can be particularly facilitated or restored. Therefore, the whitening agent of the present embodiment or the aforementioned epidermal keratinocyte proliferation promoter can particularly promote or recover the turnover of melanin-containing epidermal keratinocytes, and can cause skin blackening, spots, freckles, etc. It is particularly useful for preventing, treating or ameliorating pigmentation. Since the lavender sage extract also promotes the growth of general epidermal keratinocytes, the whitening agent of the present embodiment or the above-described epidermal keratinocyte growth promoter is a lavender squamous extract Through its growth promoting action, it can also be used for applications such as prevention, treatment or amelioration of skin aging symptoms, and regenerative medicine. However, the whitening agent of the present embodiment can be used for all purposes that are significant in exhibiting the epidermal keratinocyte proliferation promoting effect in addition to these uses.

  Further, since the whitening agent of the present embodiment has an excellent whitening effect, it is suitable for blending into, for example, a skin external preparation. In this case, the lavender sage extract may be blended as it is, or a whitening agent formulated from the lavender sage extract may be blended.

  Here, the topical skin preparation is not limited in its category, and includes a wide variety of skin cosmetics used transdermally, quasi-drugs, pharmaceuticals, and the like, in addition to the skin cosmetics described below. Specific examples include ointments, creams, emulsions, serums, lotions, packs, foundations, lip balms, bath salts, hair tonics, hair lotions, soaps, body shampoos, and the like.

  Further, since the whitening agent of the present embodiment has an excellent whitening effect, it can be suitably used as a reagent for studying the action mechanism.

(Skin cosmetics)
Lavender sage extract has an excellent whitening effect and is therefore suitable for blending into skin cosmetics. In this case, the lavender sage extract may be blended as it is, or a whitening agent formulated from the lavender sage extract may be blended. By blending the lavender sage extract or the above-mentioned whitening agent, a skin lightening agent can be provided with a whitening effect and an epidermal keratinocyte proliferation promoting effect.

  The type of lavender sage extract or a skin cosmetic in which the whitening agent can be blended is not particularly limited, and examples of the skin cosmetic include ointments, creams, emulsions, lotions, packs, foundations, and the like. .

  When blending the lavender sage extract or the whitening agent into the skin cosmetic, the amount of the blend can be appropriately adjusted according to the type of the skin cosmetic, but the preferred blending ratio is about 0.0001 to 10% by mass, and a particularly preferable compounding ratio is about 0.001 to 1% by mass in terms of a standard extract.

  The skin cosmetic of the present embodiment is a main agent, an auxiliary agent or other components used in the production of normal skin cosmetics, as long as the whitening effect of the lavender sage extract does not hinder the epidermal keratinocyte proliferation promoting effect. , Astringents, bactericidal / antibacterial agents, whitening agents, ultraviolet absorbers, humectants, cell activators, anti-inflammatory / antiallergic agents, antioxidants / active oxygen removers, oils and fats, waxes, hydrocarbons, fatty acids, Alcohols, esters, surfactants, fragrances and the like can be used in combination. The combined use as described above results in a more general product, and a synergistic effect with other active ingredients used in combination may provide an excellent effect that is more than expected.

  The skin cosmetic of the present embodiment can prevent, treat or improve pigmentation such as skin darkening, spots, freckles, etc. through the whitening effect and dermal keratinocyte proliferation promoting effect of the lavender sage extract. it can.

(Food and drink)
Lavender sage extract has an excellent whitening effect and is therefore suitable for blending in foods and drinks. In this case, the lavender sage extract may be blended as it is, or a whitening agent formulated from the lavender sage extract may be blended. By blending the above-mentioned lavender sage extract with food and drink, favorable effects of the lavender sage extract, such as an epidermal keratinocyte proliferation promoting action, can be imparted to the food and drink.

  Here, foods and drinks are foods, drugs, and quasi-drugs under the administrative divisions that are not likely to cause harm to human health and are ingested in normal social life by oral or gastrointestinal administration. It is not limited to such categories. Therefore, “foods and drinks” in the present embodiment include general foods, health foods, functional foods, health functional foods (foods for specified health use, functionally labeled foods, and nutritional functional foods) which are taken orally. Products, drugs, etc. The food or drink according to the present embodiment is preferably a food or drink that can display the preferable action of the lavender sage extract on the food or drink or its packaging, and a health functional food (specific health food, functional food). Labeled foods, nutritionally functional foods), quasi-drugs and pharmaceuticals are particularly preferred.

  When blending the above lavender sage extract or a composition formulated from the above lavender sage extract into food or drink, the amount of the active ingredient in them should be appropriately changed in consideration of the purpose of use, symptoms, sex, etc. However, in consideration of the general intake of food or drink to be added, it is preferable that the intake of the lavender sage extract per adult per day is about 1 to 1000 mg. In addition, when the food or drink to be added is a granular, tablet or capsule food or drink, the added amount of the lavender sage extract or the composition formulated from the lavender sage extract is based on the food or drink to be added. , And usually 0.1 to 100% by mass, and preferably 5 to 100% by mass in terms of the mass of the lavender sage extract.

  The food or drink of the present embodiment may be one in which the lavender sage extract is blended with any food or drink that does not hinder its activity, or a dietary supplement containing the lavender sage extract as a main component. There may be.

  When the food or drink of the present embodiment is produced, for example, saccharides such as dextrin and starch; proteins such as gelatin, soy protein, and corn protein; amino acids such as alanine, glutamine, and isoleucine; Sugars: Any auxiliary agent such as fats and oils such as soybean oil and medium-chain fatty acid triglyceride can be added to make foods and drinks of any shape.

  The food and drink to which the above-mentioned lavender sage extract can be blended are not particularly limited, and specific examples thereof include soft drinks, carbonated drinks, nutritional drinks, fruit drinks, lactic acid drinks and the like (concentrated undiluted solutions and preparations of these drinks). Ice cream, ice sorbet, shaved ice, etc .; noodles such as buckwheat, udon, harusame, gyoza skin, sweet potato skin, Chinese noodles, instant noodles; candy, chewing gum, candy, gum, chocolate, tablets Confectionery such as confectionery, snacks, biscuits, jellies, jams, creams, baked goods, etc .; processed fishery and livestock products such as kamaboko, ham, sausage; dairy products such as processed milk, fermented milk; salad oil, tempura oil, margarine, mayonnaise , Shortening, whipped cream, dressing and other fats and oils and processed fats and oils; sauces, sauces and other seasonings Soups, stews, salads, side dishes, pickles; various other forms of health and nutritional supplements; tablets, capsules, drinks, and the like. When blending the lavender sage extract with these foods and drinks, Commonly used auxiliary raw materials and additives can be used in combination.

  The whitening agent, skin cosmetic, and food and drink of the present embodiment are suitably applied to humans. However, as long as the respective effects are exhibited, animals other than humans (for example, mice, Rat, hamster, dog, cat, cow, pig, monkey, etc.).

  Hereinafter, the present invention will be described specifically with reference to test examples, but the present invention is not limited to the following examples.

[Production Example 1]
After adding 30% by volume ethanol (750 mL) to the flower part (100 g) of the lavender sage and performing extraction at 80 to 90 ° C. for 2 hours using a reflux condenser, the mixture is filtered with filter paper to obtain an extract. Was. The obtained extract was concentrated and dried under reduced pressure to obtain 20.5 g (powder) of a 30% ethanol extract of lavender sage (sample 1).

[Production Examples 2 and 3]
Lavender sage 50% ethanol extract and 80% ethanol extract were obtained in the same manner as in Production Example 1 except that the extraction solvent was changed to 50% by volume ethanol and 80% by volume ethanol, respectively (Samples 2 and 3).

  Table 1 shows the yields according to Production Examples 1 to 3.

[Test Example 1] Test for promoting keratinocyte proliferation in each of the solvent extracts (samples 1 to 3) of lavender sage obtained in Production Examples 1 to 3 Tested.

Normal human neonatal epidermal keratinocytes (NHEK) are divided into two and cultured using a growth medium for normal human epidermal keratinocytes (KGM), and then melanin is added to one of them to a concentration of 10 μg / mL. And cultured overnight. After completion of the culture, each cell was recovered by trypsinization. The collected cells were diluted with a KGM medium so as to have a cell density of 3.0 × 10 ⁴ cells / mL, and then seeded in a collagen-coated 96-well plate at 100 μL per well and cultured overnight. After completion of the culture, 100 μL of a test sample dissolved in a KGM medium (sample 1, see Table 2 below for sample concentration) was added to each well, and the cells were cultured for 3 days. In addition, it culture | cultivated similarly using the KMG culture medium without a sample as a control.

  The epidermal keratinocyte proliferation promoting effect was measured using an MTT assay. That is, after culturing for 3 days, the medium was removed, and 100 μL of MTT dissolved in a PBS buffer at a final concentration of 0.4 mg / mL was added to each well. After culturing for 2 hours, blue formazan produced in the cells was extracted with 150 μL of 2-propanol. After the extraction, the absorbance at a wavelength of 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between the two was taken as the amount of blue formazan produced. From the obtained results, the epidermal keratinocyte proliferation promotion rate (%) was calculated by the following formula for each of melanin addition and no addition. In addition, for the cells to which melanin was added, in addition to the case of melanin addition and sample addition, in addition to the epidermal keratinocyte growth promotion rate calculated by the following formula when compared with the case of melanin addition and sample addition: The growth rate when the ratio was 100% was also calculated.

Epidermal keratinocyte proliferation promotion rate (%) = St / Ct × 100
Each term in the formula represents the following.
St: The amount of blue formazan produced when the test sample was added. Ct: The amount of blue formazan produced without the addition of the sample. Table 2 shows the results. The values in parentheses for the cells to which melanin was added are the proliferation rates when the case where no melanin was added and the sample was not added was taken as 100%.

Further, in order to compare the growth promotion rates depending on the presence or absence of melanin, the growth promotion rate ratio was calculated by the following equation.
Growth promotion rate ratio (%) = A / B × 100
Each term in the formula represents the following.
A: Growth promotion rate in cells to which melanin was added B: Growth promotion rate in cells to which melanin was not added The results are shown in Table 2.

  As shown in Table 2, since the lavender sage extract (samples 1 to 3) has an excellent epidermal keratinocyte proliferation promoting action, it promotes skin turnover and is useful for preventing, treating or improving pigmentation. It was found to be effective. In particular, the extracts (samples 1 and 2) in 30% ethanol and 50% ethanol have a particularly excellent growth-promoting effect on melanin-added epidermal keratinocytes, and prevent and treat pigmentation. Or it was found to be particularly effective for improvement.

[Formulation Example 1]
According to the following composition, an emulsion was produced by a conventional method.
Lavender sage 30% ethanol extract (Production Example 1) 0.01 g
Jojoba oil 4.00g
1,3-butylene glycol 3.00 g
Arbutin 3.00 g
2.50 g of polyoxyethylene cetyl ether (20E.O.)
Olive oil 2.00g
Squalane 2.00g
Cetanol 2.00g
Glyceryl monostearate 2.00 g
2.00 g of polyoxyethylene sorbitan oleate (20E.O.)
Methyl paraoxybenzoate 0.15g
Stearyl glycyrrhetinate 0.10 g
0.10g
Dipotassium glycyrrhizinate 0.10g
Ginkgo biloba extract 0.10g
Conchiolin 0.10g
Oubaku extract 0.10g
Chamomile extract 0.10g
Spice 0.05g
Remaining purified water (total amount is 100 g)

[Formulation Example 2]
A cream having the following composition was produced by a conventional method.
Lavender sage 50% ethanol extract (Production Example 2) 0.05 g
Kujin extract 0.1g
Ougon extract 0.1g
Liquid paraffin 5.0 g
4.0g of beeswax
Squalane 10.0g
3.0 g of cetanol
Lanolin 2.0g
1.0 g of stearic acid
1.5 g of polyoxyethylene sorbitan oleate (20E.O.)
Glyceryl monostearate 3.0 g
Oil-soluble licorice extract 0.1 g
1,3-butylene glycol 6.0 g
1.5 g of methyl parahydroxybenzoate
Spice 0.1g
Remaining purified water (total amount is 100 g)

[Formulation Example 3]
A serum having the following composition was produced by a conventional method.
Lavender sage 80% ethanol extract (Production Example 3) 0.01 g
Chamomile extract 0.1g
Carrot extract 0.1g
Xanthan gum 0.3g
Hydroxyethyl cellulose 0.1 g
Carboxyvinyl polymer 0.1 g
1,3-butylene glycol 4.0 g
Dipotassium glycyrrhizinate 0.1 g
Glycerin 2.0g
Potassium hydroxide 0.25g
Spice 0.01g
Preservative (methyl paraoxybenzoate) 0.15g
2.0 g of ethanol
Remaining purified water (total amount is 100 g)

[Formulation Example 4]
A tablet having the following composition was produced by a conventional method.
Lavender sage 30% ethanol extract (Production Example 1) 5.0 mg
Dolomite (containing 20% calcium and 10% magnesium) 83.4mg
16.7mg casein phosphopeptide
Vitamin C 33.4mg
Maltitol 136.8mg
Collagen 12.7mg
Sucrose fatty acid ester 12.0mg

[Formulation Example 5]
An oral liquid preparation having the following composition was produced by a conventional method.
<Composition in 1 ampule (1 bottle 100mL)>
Lavender sage 50% ethanol extract (Production Example 2) 0.3% by mass
Sorbit 12.0% by mass
Sodium benzoate 0.1% by mass
Spice 1.0% by mass
0.5% by mass of calcium sulfate
Purified water balance (100% by mass)

  The whitening agent of the present invention can greatly contribute to the prevention or improvement of pigmentation such as skin darkening, spots, freckles and the like.

Claims (4)

Whitening agent characterized by a lavender sage extract as an active ingredient. The whitening agent according to claim 1, wherein the whitening agent is used for promoting keratinocyte proliferation.   A skin cosmetic comprising a lavender sage extract.   A food or drink comprising a lavender sage extract.