JP2016193857A - Skin cosmetic, and food and drink - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide novel compositions that are derived from natural products and have an excellent anti-aging effect, to provide anti-aging agents containing such novel compositions as active ingredients, and to provide skin cosmetics as well as food and drinks in which the novel compositions are compounded.SOLUTION: The invention provides a fermented palm sugar obtained by fermentation of palm sugar, an anti-aging agent containing fermented palm sugar as an active ingredient, and a skin cosmetic and a food and drink in which the fermented palm sugar is compounded. Fermentation, preferably, is performed by processing palm sugar aqueous solution at 25-35°C for 12-72 hours, preferably with one or more yeasts selected from the group consisting of Saccharomyces, Schizosaccharomyces, Kluyveromyces, Saccharomyces, Lachancea, and Candida. The fermented palm sugar is obtained from the fermented solution produced by fermenting the aqueous solution of palm sugar. Preferably the ethanol concentration of the fermented solution when the fermentation is finished is 1 vol.% or less.SELECTED DRAWING: None

Description

  The present invention relates to a novel composition obtained by fermentation, an anti-aging agent, a skin cosmetic, and a food and drink using the same.

  A basement membrane is present at the boundary between the epidermis and the dermis constituting the skin. The basement membrane not only connects the epidermis and dermis, but also plays an important role in maintaining skin function (see Non-Patent Document 1). The main skeleton of the basement membrane has a network structure composed of type IV collagen. Various glycoproteins mainly composed of laminin-5 are present at the boundary between the basement membrane and the epidermis and connect the basement membrane and the epidermis. Such laminin-5 is an epidermal keratinocyte present in the epidermis. More produced. In young skin, the basement membrane works to maintain the homeostatic interaction of the epidermis and dermis, ensuring moisture retention, flexibility, elasticity, and the like. Maintained.

  However, laminin-5, the main component of the basement membrane, is decomposed and affected by certain external factors such as ultraviolet irradiation, significant drying of the air, excessive skin cleansing, and so on. Alteration occurs and the basement membrane structure is destroyed (see Non-Patent Document 2). As a result, the skin's moisturizing function and elasticity are lowered, and the keratin begins to exfoliate abnormally, so the skin loses its tension and gloss, and exhibits aging symptoms such as roughness and wrinkles. As described above, changes associated with skin aging, that is, wrinkles, dullness, texture changes, decreased elasticity, etc. are associated with a decrease in basement membrane components and structural changes in the basement membrane. It is considered that skin aging symptoms can be prevented and improved by promoting production.

  Laminin consists of various combinations of α chain, β chain, and γ chain, and more than 17 types are known. Among these, laminin-5 (α3β3γ2) is abundantly present in the basement membrane of epithelial tissues such as skin, digestive organs, kidneys and lungs. In a genetic disease caused by a congenital abnormality of the gene encoding each chain of laminin-5 (lethal congenital epidermolysis bullosa, Herlitz junctional epidermolysis bullosa) It has been known. Laminin-5 is known to strongly adhere cells (high cell adhesion activity) and strongly promote cell motility (high cell motility activity) compared to other extracellular matrix molecules.

  Thus, since laminin-5 has high cell motility activity, it is known to promote cell migration in damaged skin and promote damage healing (see Patent Document 1). That is, promoting the production of laminin-5 is important in promoting the healing of skin damage that destroys the structure of the basement membrane.

  The epidermis functions to alleviate external stimuli and control the loss of body components such as moisture, and starts with the basal layer, the lowest layer, and continues to the spiny layer, granule layer, and stratum corneum. It is composed of Most cells present in each layer are keratinocytes differentiated from the basal layer. The keratinocytes that divide and proliferate in the basal layer differentiate into keratinocytes while passing through the spinous layer and the granule layer, forming a stratum corneum composed of keratin protein fibers with strong cross-linking, Eventually it will fall off the stratum corneum as plaque.

  The stratum corneum is present in the outermost shell of the skin and serves as a physical barrier against irritation from the outside world. In order to have this barrier function in the skin, the cycle (keratinization) from the production of keratinocytes in the basal layer to the peeling off of the keratinocytes (keratinization) is usually repeated at a cycle of 4 weeks to perform epidermal metabolism. . However, the metabolic function of this stratum corneum also deteriorates with aging, and skin troubles such as wrinkles, dullness, pigmentation, and rough skin occur. Therefore, it is considered that skin aging such as wrinkles, dullness, and pigmentation can be improved by promoting the proliferation of keratinocytes and restoring the metabolic function of the skin. Conventionally, as a thing which has an epidermal keratinocyte growth promotion effect, a shellfish mother extract (refer to patent documents 2) etc. are known.

  Among the basal layer, spiny layer, granule layer, and stratum corneum constituting the epidermis, in particular, in the granule layer, the cell membrane is thickened to form a thickened cell membrane, and the action of transglutaminase-1 causes protein Between molecules, glutamyl-lysine crosslinks are formed, and strong keratin protein fibers are formed. Furthermore, ceramide or the like is covalently bonded to a part of the ceramide to form a hydrophobic structure, thereby providing a foundation for the lamellar structure of the intercellular lipid and forming the basis of the keratin barrier function.

  However, when the amount of transglutaminase-1 produced in the epidermis decreases with aging, the keratin barrier function and the skin moisturizing function decrease, so that skin aging symptoms such as rough skin and dry skin may occur, or dry skin diseases ( For example, atopic dermatitis, psoriasis, ichthyosis, etc.) may develop. Therefore, it is considered that skin aging symptoms, dry skin diseases, and the like can be prevented, treated, or improved by promoting production of transglutaminase-1 in the epidermis. Extracts from Hunan cocoon tea (see Patent Document 3) and the like are known as having transglutaminase-1 production promoting action.

  In the past, it was thought that only the stratum corneum was responsible for the barrier function of the skin, but in recent years, the protein constituting the tight junction (hereinafter sometimes referred to as “TJ”) present in the epidermal granule layer is genetically encoded. It has been found that the skin barrier function is disrupted when it is deficient at the level, and TJ is also considered to play an important role in the skin barrier function (see Non-Patent Document 3). TJ is an intercellular adhesion structure that controls not only the adhesion between adjacent cells but also the permeation of substances by sealing the gaps between cells. TJ is composed of claudin and occludin, which are cell membrane proteins, and these proteins constitute the skeleton of TJ strands and are thought to control the barrier function of TJ (see Non-Patent Document 4). . Here, if the expression of claudin or occludin decreases for any reason, structural destruction of TJ occurs, and it does not function as a permeation barrier for substances, resulting in dry skin, rough skin, atopic dermatitis and various infectious diseases. It is thought to contribute to skin symptoms such as.

  Therefore, by promoting the production of claudin and occludin in the epidermis to promote TJ formation of epidermal keratinocytes, the skin barrier function and water retention function are enhanced, and dry skin, rough skin, atopic dermatitis and various infections It is thought that skin symptoms such as infectious diseases can be prevented or ameliorated. Asparasine lineias extract (patent document 4) etc. are known as having a claudin production promoting action and an occludin production promoting action.

  Glutathione is a tripeptide consisting of three amino acids, glutamic acid, cysteine and glycine, and is a compound having a major cysteine residue in the cell. Intracellular glutathione functions as a radical donor, regulation of cell function by redox, foreign body metabolism, SH donor of various enzymes, and is also known as an antioxidant component against active oxygen and the like. Its action expression is thought to be derived from cysteine residues. However, it has been reported that the amount of glutathione in cells is deficient or decreased due to excessive oxidative stress, addition of foreign substances, aging, etc., and this reduces the protective ability of cells against oxidative stress, and the cellular DNA In addition, it is considered to be a cause of damaging components such as proteins.

  Diseases induced by oxidative stress include tissue damage such as rheumatoid arthritis and Behcet's disease, various arteriosclerosis (ischemic heart disease, myocardial infarction, cerebral ischemia, cerebral infarction, etc.), neurodegenerative diseases (Alzheimer's disease) Diseases, Parkinson's disease, Huntington's chorea, etc.), lung diseases caused by cancer, smoking, etc., cataracts, diabetes, wrinkles, stiff shoulders, coldness, etc. are known. In skin, oxidative stress is considered to cause degradation of tissue such as collagen, denaturation, cross-linking, etc., or oxidation of fats and oils to produce lipid peroxides that damage cells, It is considered that a disorder caused by oxidative stress causes aging such as skin wrinkle formation and skin elasticity reduction (see Non-Patent Document 5).

  In addition to these diseases that are induced by oxidative stress as well as diseases in which a decrease or deficiency in intracellular glutathione is known to be associated with the pathological condition, liver disorders (drinking alcohol or heavy metals) Or other foreign substances such as chemical substances). That is, it is considered that promoting the production of glutathione can enhance the ability of cells to protect against oxidative stress, and can prevent and treat the above-mentioned disease group caused by the decrease or lack of intracellular glutathione level. . As a substance having a glutathione production promoting action, liquiritigenin (see Patent Document 5) and the like are known.

JP 2006-063033 A JP 2006-056854 A JP 2007-099698 A JP 2009-256244 A JP 2009-269889 A

J. Cell. Biol., 1992, Vol.119, No.3, p.695-703 J. Invest. Dermatol., 1979, Vol. 73, No. 1, p. 59-66 J. Cell Biol., 2002, vol.156, pp.1099-1111 Journal of the Japan Cosmetic Science Society, 2007, vol.31, pp.296-301 "Fragrance Journal Extra Number", 1995, No.14, p.156

  An object of the present invention is to provide a novel composition derived from a natural product and having an excellent anti-aging effect. In addition, it is a further object of the present invention to provide an anti-aging agent comprising the novel composition as an active ingredient, and a skin cosmetic and a food or drink containing the novel composition.

  In order to solve the above problems, first, the present invention provides a fermented coconut sugar product obtained by fermenting coconut sugar (Invention 1).

  In the said invention (invention 1), it is preferable that the said fermentation is performed by processing the aqueous solution of the said sucrose at 25-35 degreeC for 12 to 72 hours (invention 2).

  In the above inventions (Inventions 1 and 2), the fermentation is one or more selected from the group consisting of Saccharomyces, Schizosaccharomyces, Kluyveromyces, Digosaccharomyces, Lacansea and Candida. It is preferably performed by yeast (Invention 3).

  In the said invention (invention 1-3), the said coconut-sugar fermented material is obtained from the fermented liquor which fermented the aqueous solution of the said coconut-sugar, In the said fermented liquor when the said fermentation is complete | finished, ethanol concentration Is preferably 1% by volume or less (Invention 4).

  2ndly, this invention provides the skin cosmetics characterized by mix | blending the fermented sucrose sugar based on the said invention (invention 1-4) (invention 5).

  3rdly, this invention provides the anti-aging agent characterized by including the fermented sucrose product based on the said invention (1-4) as an active ingredient (invention 6).

  In the above invention (Invention 6), the fermented coconut sugar comprises laminin-5 production promoting action, epidermal keratinocyte proliferation promoting action, glutathione production promoting action, claudin production promoting action, occludin production promoting action, and transglutaminase. It is preferable to have one or more actions selected from the group consisting of -1 production promoting actions (Invention 7).

  4thly this invention provides the food / beverage products characterized by mix | blending the fermented sucrose sugar based on the said invention (invention 1-4) (invention 8).

  According to the present invention, a fermented coconut sugar product can be provided as a novel composition derived from a natural product and having an excellent anti-aging effect. Moreover, according to this invention, the anti-aging agent excellent in the effect can be provided by containing fermented coconut sugar as an active ingredient. Furthermore, the skin cosmetics and food-drinks excellent in the anti-aging effect can be provided by mix | blending a fermented coconut sugar product.

Embodiments of the present invention will be described below.
[Fermented coconut sugar]
The fermented coconut sugar product according to the present embodiment is obtained by fermenting coconut sugar.

  Here, unless otherwise specified, the “fermented coconut sugar product” in the present specification includes a fermented liquid obtained using coconut sugar as a fermentation raw material, a diluted or concentrated liquid of the fermented liquid, and the fermented liquid. A dry product obtained by drying, or a crude product or a purified product thereof is included.

  In the fermented coconut sugar product according to the present embodiment, the coconut sugar used as a fermentation raw material is obtained from the inflorescence or sap of an Arecaceae plant. The inflorescence liquid or sap of the palm family plant is an endosperm or a fruit liquid obtained from the fruit part of the palm family plant (for example, in the case of coconut palm, the coconut obtained from the fruit part (generally referred to as “coconut”)). Milk and coconut juice).

  Coconut palms (scientific name: Cocos nucifera L), palmyra palm (scientific name: Borassus flabellifer), sugar palm (scientific name: Arenga pinnata), nippa palm (scientific name: Nypa fruticans Wurmb), date palm (scientific name: Phoenix dactylifera), sugar beet (Scientific name: Phoenix sylvestris). Among these, from the viewpoint of availability, sugar content, and the like, coconut palm, sugar palm or sugarnut palm is preferable, and coconut palm is particularly preferable.

  The inflorescence fluid or sap of a coconut plant can be collected as a liquid that cuts off the inflorescence portion of a coconut family such as coconut or damages the bark portion and exudes therefrom. By concentrating and drying the above-mentioned inflorescence or sap, coconut sugar as a fermentation raw material in the present embodiment can be obtained.

  In such sucrose, the carbohydrate content is preferably 80 to 97% by mass, particularly preferably 85 to 95% by mass. When the sugar content of coconut sugar is within the above range, the fermentation process proceeds efficiently, and the anti-aging action (details will be described later) of the obtained coconut sugar fermentation product is particularly excellent. In the present specification, the “sugar content” of the solid content refers to the Brix value (sucrose) relative to the total solid content (% by mass) when the solid content is dissolved in water and filtered through diatomaceous earth or a membrane filter. (Corresponding to the mass% of the solution).

  In this embodiment, such coconut sugar is fermented. Fermentation treatment can be performed, for example, by dissolving concentrated and dried coconut sugar in water and inoculating the bacteria to be fermented. The amount of sucrose used is preferably 0.05 to 15%, more preferably 0.1 to 10% in terms of the Brix value of the sucrose aqueous solution.

  Examples of the bacteria to be fermented include Saccharomyces cerevisiae (Saccharomyces cerevisiae, baker's yeast), Saccharomyces veronae and other Saccharomyces genera; Schizosaccharomyces pombe Genus; Kluyveromyces thermotolerans and other genus Kluyveromyces; Zygosaccharomyces thermotolerans and others Zygosaccharomyance Preferred examples include yeasts such as Candida genus such as Candida utilis (torula yeast). In addition, lactic acid bacteria such as Lactobacillus plantarum and Lactobacillus casei, such as Lactobacillus casei, may also be used. In this embodiment, these bacteria can be used individually by 1 type or in combination of 2 or more types. Among these, yeasts are preferable from the viewpoint of safety when used in skin cosmetics and foods and drinks, yeasts of the genus Saccharomyces or Lacansea are particularly preferable, and Saccharomyces veronae (also known as Lacancea thermotolerance). (Lachancea thermotolerans)) is more preferred.

  Such fermentation treatment is performed, for example, by placing an aqueous solution of sucrose sugar and the yeast in a fermenter and treating at a temperature range of 25 to 35 ° C., preferably 28 to 32 ° C., for 12 to 72 hours, preferably 18 to 48 hours. This can be done. In addition, when the pH of the reaction solution is adjusted to 5.0 to 7.0, preferably 5.5 to 6.5 at the start of the fermentation process, the fermentation process suitably proceeds. Such fermentation treatment is terminated by subjecting the fermentation broth to desired means such as cooling, heat sterilization, and filtration, and inactivating the fermenting bacteria.

  In the fermentation broth when the fermentation treatment is terminated, the ethanol concentration is preferably 1% by volume or less, and more preferably 0.5% by volume or less. Here, 1% by volume or less includes 0% by volume, that is, the case where ethanol is not contained at all in the fermentation broth. Moreover, it is preferable that the said fermentation is mainly performed by aerobic respiration and substantially no alcohol fermentation is performed. The fermented coconut sugar product obtained by substantially not performing alcoholic fermentation has a particularly excellent anti-aging effect described below.

  Moreover, it is preferable that it is 3.0-4.5, and, as for pH of a fermented liquid when finishing a fermentation process, it is more preferable that it is 3.5-4.0. When the fermentation process proceeds sufficiently, an organic acid such as lactic acid is generated, and the pH decreases. Therefore, it can be said that the fermentation broth in the range where the pH is applied has been sufficiently advanced in the fermentation treatment, and the fermented coconut sugar obtained from such a fermentation broth has a particularly excellent anti-aging effect described later. Become.

  The fermentation broth thus obtained may be used as it is as a fermented product, or may be appropriately diluted or concentrated and used as a fermented product. Furthermore, the concentrate may be further dried, and crude purification or the like may be performed.

  In the fermented coconut sugar product obtained as described above, the carbohydrate content is preferably 0% by mass or more and less than 80% by mass, and more preferably 30 to 70% by mass. Here, “0% by mass or more” includes a case where the Brix value in the fermented product dissolved in water is 0%, and all saccharides are consumed by the fermentation treatment and do not remain in the fermented product. A fermented product having a saccharide content in the above range can be said to have undergone sufficient fermentation treatment, and has an especially excellent anti-aging effect described below.

  In the fermented coconut sugar product, the content of lactic acid is preferably 0.001 to 0.1% by mass, and more preferably 0.005 to 0.05% by mass in terms of solid content.

  Since the above fermented coconut sugar exhibits an excellent anti-aging action, it is particularly suitable as an active ingredient of an anti-aging agent described later and as a blending ingredient for skin cosmetics and foods and drinks.

[Anti-aging agent]
The anti-aging agent which concerns on this embodiment contains the fermented sucrose sugar obtained as mentioned above as an active ingredient. The anti-aging agent of this embodiment can be used for wide uses, such as a pharmaceutical, a quasi-drug, and cosmetics.

  Anti-aging effects of fermented coconut sugar include, for example, laminin-5 production promoting action, epidermal keratinocyte proliferation promoting action, glutathione production promoting action, claudin production promoting action, occludin production promoting action, and transglutaminase-1 ( TG-1) It is exhibited based on one or more actions selected from the group consisting of production promotion actions. However, the anti-aging action which fermented coconut sugar has is not limited to the anti-aging action exhibited based on the above action.

  In addition, fermented coconut sugar utilizes its laminin-5 production promoting action, epidermal keratinocyte proliferation promoting action, glutathione production promoting action, claudin production promoting action, occludin production promoting action, or TG-1 production promoting action. And laminin-5 production promoter, epidermal keratinocyte proliferation promoter, glutathione production promoter, claudin production promoter, occludin production promoter, or transglutaminase-1 production promoter, respectively. Good.

  The anti-aging agent of this embodiment may consist only of fermented coconut sugar, or may be formulated from fermented coconut sugar.

  The anti-aging agent of the present embodiment is an arbitrary agent such as powder, granule, tablet, liquid, etc. according to a conventional method using a pharmaceutically acceptable carrier such as dextrin and cyclodextrin and any other auxiliary agent. It can be formulated into a form. In this case, as an auxiliary agent, for example, an excipient, a binder, a disintegrant, a lubricant, a stabilizer, a flavoring / flavoring agent, and the like can be used. The anti-aging agent can be used by blending with other compositions (for example, skin cosmetics, etc.), and can also be used as an ointment, a liquid for external use, a patch, and the like.

  When the anti-aging agent of this embodiment is formulated, the content of fermented coconut sugar is not particularly limited, and can be appropriately set according to the purpose.

  In addition, the anti-aging agent of this embodiment can mix | blend other natural extracts etc. which have an anti-aging effect with an fermented sucrose sugar as needed, and can use it as an active ingredient.

  Examples of the administration method of the anti-aging agent of this embodiment to a patient include transdermal administration, oral administration, and the like, and a suitable method for the prevention / treatment or the like may be appropriately selected according to the type of disease. In addition, the dose of the anti-aging agent of the present embodiment may be appropriately increased or decreased depending on the type of disease, severity, individual differences among patients, administration method, administration period, and the like.

  The anti-aging agent of the present embodiment includes laminin-5 production promoting action, epidermal keratinocyte proliferation promoting action, glutathione production promoting action, claudin production promoting action, occludin production promoting action, which the fermented coconut sugar as an active ingredient has, And, through one or more actions selected from the group consisting of TG-1 production promoting action, prevent, treat or ameliorate skin aging symptoms such as skin wrinkle formation, reduced elasticity, reduced moisturizing function, etc. be able to. However, in addition to these uses, the anti-aging agent of the present embodiment is not limited to laminin-5 production, epidermal keratinocyte proliferation promotion, glutathione production promotion, claudin production promotion, occludin production promotion, or TG -1 production promoting action can be used for all purposes meaningful.

  For example, the anti-aging agent of the present embodiment or the laminin-5 production promoter described above induces reconstruction of the basement membrane structure through the laminin-5 production promotion action of the fermented coconut sugar to treat wounds in the skin. Can be improved. Moreover, the anti-aging agent of this embodiment or the laminin-5 production promoter described above can be used as a prophylactic or therapeutic agent for diseases (such as epidermolysis bullosa) caused by laminin-5 deficiency (deficiency).

  In addition to the above-mentioned uses, the anti-aging agent of the present embodiment or the above-mentioned epidermal keratinocyte proliferation promoter restores skin metabolism through the epidermal keratinocyte proliferation promoting action of the fermented sugar sugar, It can be used for applications such as prevention, treatment or improvement of dullness, pigmentation, etc .; regenerative medicine;

  In addition to the uses described above, the anti-aging agent of the present embodiment or the glutathione production promoter described above is capable of promoting tissue glutamate production, such as rheumatoid arthritis and Behcet's disease, and various arteriosclerosis (false Blood heart disease, myocardial infarction, cerebral ischemia, cerebral infarction, etc.), neurodegenerative diseases (Alzheimer's disease, Parkinson's disease, Huntington's chorea, etc.), lung disease caused by cancer, smoking, cataract, diabetes, wrinkles, stiff shoulders Diseases induced by oxidative stress such as coldness; decreased or deficient intracellular glutathione levels such as liver damage (caused by excessive drinking of alcohol or ingestion of foreign substances such as heavy metals and chemicals) It is possible to prevent, treat or ameliorate a disease known to be associated with a disease state.

  In addition to the above-described uses, the anti-aging agent of the present embodiment, or the above-mentioned claudin production promoter or occludin production promoter is capable of forming tight junctions in epidermal keratinocytes through the claudin production promoting action of fermented coconut sugar. Thus, the skin barrier function and moisture retention function can be enhanced, and skin symptoms such as dry skin, rough skin, atopic dermatitis and various infectious diseases can be prevented or improved.

  In addition to the applications described above, the anti-aging agent of the present embodiment or the above-described TG-1 production promoter enhances the barrier function of the skin through the TG-1 production promotion action of the fermented coconut sugar, resulting in rough skin and dry skin. In addition, dry skin diseases (for example, atopic dermatitis, psoriasis, ichthyosis, etc.) can be prevented, treated or ameliorated.

  Moreover, since the anti-aging agent of this embodiment has the outstanding anti-aging effect, it is suitable for mix | blending with a skin external preparation, for example. In this case, the fermented coconut sugar may be blended as it is, or an anti-aging agent formulated from the fermented coconut sugar may be blended. Here, the external preparation for skin is not limited in its category, and includes a wide range of quasi-drugs and pharmaceuticals used transdermally, in addition to the skin cosmetics described below.

  Moreover, since the anti-aging agent of this embodiment has the outstanding anti-aging effect | action, it can be utilized suitably also as a reagent for the research regarding these action mechanisms.

[Skin cosmetic]
The skin cosmetic according to the present embodiment is blended with the above-described fermented coconut sugar. In the present embodiment, the fermented coconut sugar product may be blended as it is, or an anti-aging agent formulated from the fermented coconut sugar product may be blended.

  By blending a fermented coconut sugar product or the above-mentioned anti-aging agent, the skin cosmetic preparation has an anti-aging effect, a laminin-5 production promoting effect, an epidermal keratinocyte proliferation promoting effect, a glutathione production promoting effect, a claudin production promoting effect, occludin A production promoting action or a TG-1 production promoting action can be imparted.

  The kind of coconut sugar fermented product or the skin cosmetic that can be mixed with the anti-aging agent is not particularly limited, and examples of the skin cosmetic include ointments, creams, emulsions, lotions, packs, foundations and the like. It is done.

  When the fermented coconut sugar product or the anti-aging agent is blended in the skin cosmetic, the blending amount can be appropriately adjusted according to the type of the skin cosmetic, but the preferred blending ratio is about 0.0001. A particularly suitable blending ratio is about 0.001 to 1% by mass in terms of a standard extract.

  The skin cosmetic of the present embodiment has an anti-aging action, laminin-5 production promoting action, epidermal keratinocyte proliferation promoting action, glutathione production promoting action, claudin production promoting action, occludin production promoting action, which the fermented coconut sugar has, Or as long as the TG-1 production promoting action is not hindered, main agents, auxiliaries or other components used in the production of normal skin cosmetics, such as astringents, bactericides / antibacterial agents, whitening agents, UV absorbers, moisturizers , Cell activators, anti-inflammatory / antiallergic agents, antioxidant / active oxygen scavengers, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, perfumes, etc. . When used in combination, it becomes a more general product, and a synergistic effect with other active ingredients used in combination may lead to an excellent effect that is more than normally expected.

  The skin cosmetic of the present embodiment has an anti-aging action, laminin-5 production promoting action, epidermal keratinocyte proliferation promoting action, glutathione production promoting action, claudin production promoting action, occludin production promoting action, which the fermented coconut sugar has, And prevention or treatment of contact dermatitis (rash), psoriasis, pemphigus vulgaris, and other various skin inflammatory diseases associated with rough skin through one or more actions selected from the group consisting of TG-1 production promoting action Or prevention; prevention, treatment or improvement of skin darkening, pigmentation such as spots, buckwheat, etc .; prevention, treatment or improvement of skin aging symptoms such as formation of skin wrinkles, reduced elasticity, reduced moisturizing function; Promotion of healing of wounds or burns; prevention, treatment or amelioration of dry skin diseases (eg, atopic dermatitis, psoriasis, ichthyosis, etc.) as well as rough skin, dry skin, etc .; obesity, and Can be like; arteriosclerosis associated therewith, diabetes, prevention of lifestyle-related diseases such as metabolic syndrome, treatment or amelioration.

[Food and Drink]
The food / beverage products which concern on this embodiment mix | blend the coconut sugar fermented product mentioned above. In the present embodiment, the fermented coconut sugar product may be blended as it is, or an anti-aging agent formulated from the fermented coconut sugar product may be blended.

  Here, food and drink are those that are less likely to harm human health and are taken by oral or gastrointestinal administration in normal social life. It is not limited to such categories. Therefore, the “food and drink” in the present embodiment includes health foods (functional foods and drinks) that are taken orally, health functional foods (special health foods, functional indication foods, nutritional functional foods, etc.), quasi-drugs Products, pharmaceuticals, etc. Among these, since the function of fermented coconut sugar can be displayed, it is preferably a health functional food, a quasi-drug, or a pharmaceutical.

  When blending a fermented coconut sugar product or an anti-aging agent formulated from a fermented coconut sugar product with food and drink, the amount of the active ingredient in the product may be changed as appropriate in consideration of the purpose of use, symptoms, sex, etc. However, in consideration of the general intake of foods and drinks to be added, it is preferable that the daily intake of the extract is about 1-1000 mg. In addition, when the food or drink to be added is a granular, tablet or capsule food or drink, the added amount of the citrus sugar fermented product or the anti-aging agent formulated from the fermented sugar fermented product is relative to the food and drink to be added. Usually, it is 0.1-100 mass%, Preferably it is 5-100 mass%.

  The food / beverage products of this embodiment may be a product obtained by blending a fermented coconut sugar product with any food / beverage product that does not impede its activity, or a dietary supplement (supplement) based on a fermented coconut sugar product. It may be.

  When producing the food and drink of this embodiment, for example, sugars such as dextrin and starch; proteins such as gelatin, soybean protein and corn protein; amino acids such as alanine, glutamine and isoleucine; Arbitrary auxiliary agents, such as saccharides; oils and fats, such as soybean oil and medium chain fatty acid triglyceride, can be added, and it can be set as food / beverage products of arbitrary shapes.

  Foods and drinks that can be blended with the fermented coconut sugar are not particularly limited, and specific examples thereof include tablets, capsules, drinks; and other various forms of health and nutritional supplements. In addition, as functional health foods that display the function of fermented coconut sugar (food for specified health use, functional indication food, nutritional functional food), beverages such as soft drinks, carbonated drinks, nutritional drinks, fruit drinks, and lactic acid drinks ( Concentrated concentrates of these beverages and preparation powder); Ice cream, ice sherbet, shaved ice and other frozen desserts; buckwheat, udon, harsame, gyoza skin, cucumber skin, Chinese noodles, instant noodles and other noodles; Chewing gum, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream, baked confectionery, etc .; fishery and livestock processed foods such as kamaboko, ham, sausage; dairy products such as processed milk, fermented milk Oil and fat processed foods such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressing; Scan, seasonings such as sauce; soups, stews, salads, prepared foods, pickles; palm sugar fermentations may be blended in like. When blending a fermented coconut sugar product with these foods and drinks, commonly used auxiliary raw materials and additives can be used in combination.

  In addition, although the anti-aging agent, skin cosmetics, and food / beverage products of this embodiment are suitably applied with respect to a human, as long as each effect is show | played, animals other than a human (for example, mouse | mouth) , Rat, hamster, dog, cat, cow, pig, monkey, etc.).

  Hereinafter, although a manufacture example and a test example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to each following example at all.

[Production Example 1] Production of fermented coconut sugar As coconut sugar, 100 g of coconut sugar (manufactured by Tipco) obtained from coconut inflorescence solution was dissolved in 1000 g of water and sterilized by heating (110 ° C, 5 minutes). did. The obtained aqueous solution had a pH of 6.0, an ethanol concentration of 0% by volume (below the detection limit), and a lactic acid content of 0% by mass (below the detection limit). The aqueous solution was filtered through diatomaceous earth, and the Brix value of the filtrate was measured and found to be 8.4%. The pH was measured using a pH meter (manufactured by Toa DKK Corporation), and the Brix value was measured using a saccharimeter (manufactured by Atago Co., Ltd.). The ethanol concentration was measured by gas chromatography, and the lactic acid content was measured by high performance liquid chromatography under the conditions described later. The same applies to the following.

  To this aqueous solution, 0.1 g of yeast for fermentation (Saccharomyces verona, manufactured by Akita Imano Shoten) was added, put into a flask, and allowed to stand at 30 ° C. for 24 hours for fermentation treatment. The obtained fermentation stock solution had a pH of 3.6, a Brix value of 5.0%, and an ethanol concentration of 0.58% by volume. The fermented broth was filtered through diatomaceous earth and further heat sterilized (110 ° C., 5 minutes) to obtain 1000 g of coconut sugar fermented broth. In the obtained sucrose fermentation liquid, the solid content was 8.0% by mass, the pH was 3.5, and the Brix value was 5.0%. The coconut sugar fermentation broth was concentrated and dried to obtain a coconut sugar fermentation product (80 g, sample 1).

  In addition, in the obtained fermented coconut sugar product (sample 1), the carbohydrate content measured by a sugar content meter (manufactured by Atago Co., Ltd.) was 61.0% by mass, and the lactic acid content measured by high performance liquid chromatography was 0. 110% by mass.

  The conditions for gas chromatography for measuring the ethanol concentration and the conditions for high-performance liquid chromatography for measuring the lactic acid content are as shown below.

<Gas chromatography conditions>
Equipment used: GC-2014 (manufactured by Shimadzu Corporation)
Column: DB5 (manufactured by Agilent Technologies)
Column temperature: 40 ° C
Inlet temperature: 200 ° C
Detector: Hydrogen flame detector (FID)
Sample injection volume: 1 μL
Carrier gas: Nitrogen gas Carrier gas flow rate: 1.2 mL / min

<High performance liquid chromatography conditions>
Equipment used: Agilent 1200 series (manufactured by Agilent Technologies)
Column: Wakosil II 5C18HG (manufactured by Agilent Technologies)
Mobile phase: 0.85% phosphoric acid Mobile phase flow rate: 1.0 mL / min
Detection: 210nm

[Production Example 2] Production of fermented coconut sugar The fermented liquid obtained by fermentation treatment was sterilized by heating (110 ° C, 5 minutes) and then filtered through diatomaceous earth in the same manner as in Production Example 1 and then fermented coconut sugar. 1000 g was obtained (solid content: 8.0 mass%, pH: 3.5, Brix value: 5.0%). The coconut sugar fermentation broth was concentrated and dried to obtain a coconut sugar fermentation product (80 g, sample 2).

  In addition, in the obtained fermented coconut sugar product (sample 2), the carbohydrate content measured by a sugar content meter (manufactured by Atago Co., Ltd.) was 62.5% by mass, and the lactic acid content measured by high performance liquid chromatography was 0. It was 125 mass%.

[Test Example 1] Laminin-5 production promoting action test Laminin-5 production promoting action was tested in the following manner for the fermented sugar sugar product (sample 1) obtained in the production example.

Normal human neonatal epidermal keratinocytes (NHEK) were cultured using a growth medium for normal human epidermal keratinocytes (KGM), and then cells were collected by trypsin treatment. The collected cells are diluted with a medium (KGM-BPE) obtained by removing BPE from KGM so that the cell density becomes 1 × 10 ⁵ cells / mL, and then seeded at 500 μL per well in a 24-well plate for 1 day. Cultured.

  After completion of the culture, the medium was removed, and 500 μL of a test sample (see Table 1 below for the sample concentration) dissolved in the KGM-BPE medium was added to each well and cultured for 48 hours. In addition, it culture | cultivated similarly using the KGM-BPE culture medium without a sample as control. After completion of the culture, 100 μL of the culture supernatant was transferred to an ELISA plate and adsorbed on the plate at 37 ° C. for 2 hours. Then, the amount of adsorbed laminin-5 was determined using monoclonal anti-human laminin-5 antibody (mouse IgG) (Chemicon). Measured by ELISA method. From the obtained measurement results, the laminin-5 production promotion rate (%) was calculated by the following formula.

Laminin-5 production promotion rate (%) = A / B × 100
Each term in the formula represents the following.
A: Laminin-5 amount with addition of test sample B: Laminin-5 amount without addition of sample Table 1 shows the results.

  As shown in Table 1, it was confirmed that the fermented coconut sugar (sample 1) has an excellent laminin-5 production promoting action. In addition, the fermented coconut sugar product (sample 2) obtained in Production Example 2 also had an excellent laminin-5 production promoting action (data not shown).

[Test Example 2] Epidermal keratinocyte proliferation promoting action test The fermented sugar sugar product (sample 1) obtained in the production example was tested for the epidermal keratinocyte proliferation promoting action as follows.

Normal human neonatal epidermal keratinocytes (NHEK) were cultured using a normal human epidermal keratinocyte long-term culture growth medium (EpiLife-KG2), and then cells were collected by trypsin treatment. The collected cells were diluted with the above medium to a cell density of 3.0 × 10 ⁴ cells / mL, then seeded at 100 μL per well in a collagen-coated 96-well plate, and cultured overnight. After completion of the culture, 100 μL of a test sample dissolved in EpiLife-KG2 medium (see Table 2 below for sample concentration) was added to each well and cultured for 3 days. In addition, it culture | cultivated similarly using the EpiLife-KG2 culture medium without a sample as control.

  The epidermal keratinocyte proliferation promoting action was measured using the MTT assay. That is, after culturing for 3 days, the medium was removed, and 100 μL of MTT dissolved in PBS (−) buffer at a final concentration of 0.4 mg / mL was added. After culturing for 2 hours, blue formazan produced in the cells was extracted with 100 μL of 2-propanol. After extraction, the absorbance at a wavelength of 570 nm was measured. At the same time, the absorbance at a wavelength of 650 nm was measured as turbidity, and the difference between the two was used as the amount of blue formazan produced. From the obtained results, the epidermal keratinocyte proliferation promotion rate (%) was calculated by the following formula.

Epidermal keratinocyte proliferation promotion rate (%) = St / Ct × 100
Each term in the formula represents the following.
St: Blue formazan production amount with addition of test sample Ct: Blue formazan production amount without addition of sample Table 2 shows the results.

  As shown in Table 2, it was confirmed that the fermented coconut sugar (sample 1) has an excellent epidermal keratinocyte proliferation promoting action. In addition, the fermented coconut sugar product (sample 2) obtained in Production Example 2 also had an excellent epidermal keratinocyte proliferation promoting action (data not shown).

[Test Example 3] Glutathione production promoting action test The fermented sugar sugar product (sample 1) obtained in the production example was tested for glutathione production promoting action as follows.

Normal human neonatal epidermal keratinocytes (NHEK) were cultured using a normal human epidermal keratinocyte long-term culture growth medium (EpiLife-KG2), and then cells were collected by trypsin treatment. The collected cells were diluted with the above medium to a cell density of 1.0 × 10 ⁵ cells / mL, and then seeded on a collagen-coated 24-well plate at 500 μL per well and cultured overnight.

  After completion of the culture, 400 μL of a test sample dissolved in EpiLife-KG2 medium (see Table 3 below for the sample concentration) was added to each well and further cultured for 24 hours. In addition, it culture | cultivated similarly using the EpiLife-KG2 culture medium without a sample as control. After completion of the culture, the medium was removed from each well, washed with 1 mL of PBS (−) buffer, and cells were lysed using 150 μL of M-PER (PIERCE).

  Of these, 100 μL was used to quantify total glutathione. Specifically, 100 μL of dissolved cell extract, 50 μL of 0.1 M phosphate buffer, 2 μM NADPH, and 25 μL of glutathione reductase (final concentration: 17.5 unit / mL) were added to a 96-well plate, and the mixture was heated at 37 ° C. for 10 minutes. 25 μL of 10 mM 5,5′-dithiobis (2-nitrobenzoic acid) was added, and the absorbance at a wavelength of 412 nm until 5 minutes later was measured to obtain ΔOD / min. The total glutathione concentration was calculated based on a calibration curve prepared using oxidized glutathione (manufactured by Wako Pure Chemical Industries, Ltd.). After correcting the obtained value to the amount of glutathione per total protein amount, the glutathione production promotion rate (%) was calculated by the following formula.

Glutathione production promotion rate (%) = B / A × 100
Each term in the formula represents the following.
A: Glutathione amount per total protein amount without addition of sample B: Glutathione amount per total protein amount with addition of test sample Table 3 shows the results.

  As shown in Table 3, it was recognized that the fermented coconut sugar (sample 1) has an excellent glutathione production promoting action. The fermented coconut sugar product (sample 2) obtained in Production Example 2 was the same (data not shown).

[Test Example 4] Claudin-1 production promoting action test The clathin-1 fermented product (sample 1) obtained in the production example was tested for claudin-1 production promoting action as follows.

Normal human newborn epidermal keratinocytes (NHEK) were cultured using normal human epidermal keratinocyte growth medium (KGM), and then cells were collected by trypsin treatment. The collected cells were diluted with the above medium to a cell density of 2 × 10 ⁵ cells / mL, then seeded at 100 μL per well in a 96-well plate, and cultured overnight.

  After completion of the culture, 100 μL of a test sample dissolved in the KGM medium (see Table 4 below for sample concentration) was added to each well and cultured for 24 hours. As a control, the same culture was performed using a sample-free KGM medium. After completion of the culture, the medium was removed, the cells were fixed to the plate, and the amount of claudin-1 expressed on the cell surface was determined using a polyclonal anti-human claudin-1 antibody (rabbit IgG) (Life Technologies). It measured by ELISA method. From the obtained results, the claudin-1 production promotion rate (%) was calculated by the following formula.

Claudin-1 production promotion rate (%) = A / B × 100
Each term in the formula represents the following.
A: Claudin-1 amount with addition of test sample B: Claudin-1 amount without addition of test sample Table 4 shows the results.

  As shown in Table 4, it was confirmed that the fermented coconut sugar (sample 1) has an excellent claudin-1 production promoting action. The fermented coconut sugar product (sample 2) obtained in Production Example 2 was the same (data not shown).

[Test Example 5] Occludin production promoting action test The octopus sugar fermentation product (sample 1) obtained in the production example was tested for the occludin production promoting action as follows.

Normal human neonatal epidermal keratinocytes (NHEK) were cultured using a growth medium for normal human epidermal keratinocytes (KGM), and then cells were collected by trypsin treatment. The collected cells were diluted with the above medium to a cell density of 2 × 10 ⁵ cells / mL, then seeded at 100 μL per well in a 96-well plate, and cultured overnight.

  After completion of the culture, 100 μL of a test sample dissolved in the KGM medium (see Table 5 below for sample concentration) was added to each well and cultured for 24 hours. As a control, the same culture was performed using a sample-free KGM medium. After completion of the culture, the medium was removed, the cells were fixed to the plate, and the amount of occludin expressed on the cell surface was measured by ELISA using a polyclonal anti-human occludin antibody (rabbit IgG) (Life Technologies). From the obtained measurement results, the occludin production promotion rate (%) was calculated by the following formula.

Occludin production promotion rate (%) = A / B × 100
Each term in the formula represents the following.
A: Amount of occludin with addition of test sample B: Amount of occludin without addition of test sample The results are shown in Table 5.

  As shown in Table 5, it was confirmed that the fermented coconut sugar (sample 1) has an excellent occludin production promoting action. The fermented coconut sugar product (sample 2) obtained in Production Example 2 was the same (data not shown).

[Test Example 6] Transglutaminase-1 production promoting action test The fermented sugar sugar product (sample 1) obtained in the production example was tested for the transglutaminase-1 production promoting action as follows.

Normal human neonatal epidermal keratinocytes (NHEK) were cultured using a growth medium for normal human epidermal keratinocytes (KGM), and then cells were collected by trypsin treatment. The collected cells were diluted with the above medium to a cell density of 1 × 10 ⁵ cells / mL, then seeded at 100 μL per well in a 96-well plate, and cultured for 2 days.

  After completion of the culture, 100 μL of a test sample dissolved in KGM medium (see Table 6 below for the sample concentration) was added to each well and cultured for 24 hours. As a control, the same culture was performed using a sample-free KGM medium. After completion of the culture, the medium is removed, the cells are fixed to the plate, and the amount of transglutaminase-1 expressed on the cell surface is determined by using monoclonal anti-human transglutaminase-1 antibody (mouse IgG) (manufactured by Biomedical Technologies Inc.). It was measured by the ELISA method used. From the obtained measurement results, the transglutaminase-1 production promotion rate (%) was calculated by the following formula.

Transglutaminase-1 production promotion rate (%) = A / B × 100
Each term in the formula represents the following.
A: Transglutaminase-1 amount with addition of test sample B: Transglutaminase-1 amount without addition of sample Table 6 shows the results.

  As shown in Table 6, it was recognized that the fermented coconut sugar (sample 1) has an excellent transglutaminase-1 production promoting action. The fermented coconut sugar product (sample 2) obtained in Production Example 2 was the same (data not shown).

[Formulation Example 1]
A milky lotion was produced by a conventional method according to the following composition.
0.01g fermented coconut sugar
Jojoba oil 4.00 g
1,3-butylene glycol 3.00 g
Arbutin 3.00g
Polyoxyethylene cetyl ether (20E.O.) 2.50g
2.00 g of olive oil
Squalane 2.00g
Cetanol 2.00g
Glyceryl monostearate 2.00g
Oleic acid polyoxyethylene sorbitan (20E.O.) 2.00g
Methyl paraoxybenzoate 0.15g
Stearyl glycyrrhizinate 0.10g
Twilight extract 0.10g
Dipotassium glycyrrhizinate 0.10g
Ginkgo biloba extract 0.10g
Conchiolin 0.10g
Oat extract 0.10g
Chamomile extract 0.10g
Fragrance 0.05g
Purified water remainder (total amount is 100 g)

[Formulation Example 2]
A cream having the following composition was produced by a conventional method.
Fermented coconut sugar 0.05g
Kujin extract 0.1g
Ogon Extract 0.1g
Liquid paraffin 5.0g
Salami beeswax 4.0g
Squalane 0.0g
Cetanol 3.0g
Lanolin 2.0g
Stearic acid 1.0g
Oleic acid polyoxyethylene sorbitan (20E.O.) 1.5g
3.0 g glyceryl monostearate
Oil soluble licorice extract 0.1g
1,3-butylene glycol 6.0 g
1.5 g of methyl paraoxybenzoate
Fragrance 0.1g
Purified water remainder (total amount is 100 g)

[Composition Example 3]
A serum having the following composition was produced by a conventional method.
0.01g fermented coconut sugar
Chamomile extract 0.1g
Carrot extract 0.1g
Xanthan gum 0.3g
Hydroxyethylcellulose 0.1g
Carboxyvinyl polymer 0.1g
1,3-butylene glycol 4.0 g
0.1g dipotassium glycyrrhizinate
Glycerin 2.0g
Potassium hydroxide 0.25g
Fragrance 0.01g
Preservative (Methyl paraoxybenzoate) 0.15g
Ethanol 2.0g
Purified water remainder (total amount is 100 g)

[Formulation Example 4]
A hair tonic having the following composition was produced by a conventional method.
0.4 g of fermented coconut sugar
Tocopherol acetate appropriate amount Cephalatin 0.002g
Isopropylmethylphenol 0.1g
Sodium hyaluronate 0.15g
Glycerin 15.0g
15.0 g of ethanol
Fragrance Appropriate amount Chelating agent (Sodium edetate) Appropriate amount Preservative (hinokitiol) Appropriate amount Solubilizer (Polyoxyethylene cetyl ether) Appropriate amount Purified water The remainder (100% total)

[Formulation Example 5]
A shampoo having the following composition was produced by a conventional method.
Fermented coconut sugar 0.5g
Marjoram extract 1.0g
Plum fruit part extract 0.2g
Coconut oil fatty acid methyl taurine sodium 10.0g
Coconut oil fatty acid amidopropyl betaine 10.0g
Sodium polyoxyethylene alkyl ether sulfate 20.0g
Coconut oil fatty acid diethanolamide 4.0g
Propylene glycol 2.0g
Perfume Appropriate amount Purified water The remainder (100g total amount)

[Composition Example 6]
A tablet having the following composition was produced by a conventional method.
Fermented coconut sugar 5.0mg
Dolomite (containing 20% calcium and 10% magnesium) 83.4mg
Casein phosphopeptide 16.7mg
Vitamin C 33.4mg
Maltitol 136.8mg
Collagen 12.7mg
Sucrose fatty acid ester 12.0mg

[Formulation Example 7]
By an ordinary method, an oral liquid preparation having the following composition was produced.
<Composition in one ampoule (one 100 mL)>
Fermented coconut sugar 0.3% by mass
Sorbit 12.0% by mass
Sodium benzoate 0.1% by mass
Fragrance 1.0% by mass
Calcium sulfate 0.5% by mass
Purified water balance (100% by mass)

  The anti-aging agent of the present invention can greatly contribute to prevention, treatment or improvement of skin aging symptoms; promotion of healing of wounds or burns; prevention, treatment or improvement of dry skin diseases;

Claims (8)

  Fermented coconut sugar fermented with coconut sugar.   The fermented coconut sugar product according to claim 1, wherein the fermentation is performed by treating the aqueous solution of coconut sugar at 25 to 35 ° C for 12 to 72 hours.   The fermentation is performed by one or more yeasts selected from the group consisting of Saccharomyces, Schizosaccharomyces, Kluyveromyces, Digosaccharomyces, Lacansea and Candida. Item 3. A fermented coconut sugar product according to item 1 or 2.   The fermented coconut sugar product is obtained from a fermented liquid obtained by fermenting the aqueous solution of coconut sugar, and the ethanol concentration in the fermented liquid when the fermentation is terminated is 1% by volume or less. The fermented coconut sugar product according to any one of claims 1 to 3.   A skin cosmetic comprising the fermented coconut sugar product according to any one of claims 1 to 4.   An anti-aging agent comprising the fermented coconut sugar product according to any one of claims 1 to 4 as an active ingredient.   The fermented coconut sugar product is composed of laminin-5 production promoting action, epidermal keratinocyte growth promoting action, glutathione production promoting action, claudin production promoting action, occludin production promoting action, and transglutaminase-1 production promoting action. The anti-aging agent according to claim 6, which has one or more selected actions.   A food or drink comprising the fermented coconut sugar product according to any one of claims 1 to 4.