JP2019006700A - Anti-aging agent, hair-growing agent, anti-obesity agent, cosmetic, and food/drink product - Google Patents

Abstract

To provide an anti-aging agent with excellent anti-aging action, a hair-growing agent with excellent hair-growing action, and an anti-obesity agent with excellent anti-obesity action, and a cosmetic and a food/drink product containing at least one of the agents.SOLUTION: There are provided an anti-aging agent, a hair-growing agent, and an anti-obesity agent which contain a yeast fungus fermented product produced by fermenting sake lees extract liquid by yeast fungus. There are provided a cosmetic and a food/drink product for at least one kind selected from anti-aging use containing an anti-aging agent, hair-growing use containing a hair-growing agent, anti-obesity use containing an anti-obesity agent. It is a preferable aspect that the yeast fungus is genus Saccharomyces veronae.SELECTED DRAWING: None

Description

  The present invention relates to an anti-aging agent, a hair restorer, an anti-obesity agent, a cosmetic, and a food and drink.

  Sake, shochu, beer, and the like are known as alcoholic fermented alcoholic beverages, and it has been reported that the sake produced as a by-product during the production of these alcoholic beverages has various effects.

  The effects of sake lees (hereinafter also referred to as “sake lees”) include, for example, diabetes, hypertension, osteoporosis, cerebral infarction, angina pectoris, myocardial infarction, prevention of arteriosclerosis, improvement of allergic constitution, and It is known to have a whitening effect.

  Furthermore, a method using a fermented product of sake lees is known, and for example, a cosmetic containing a fermented product of sake yeast with yeast has been proposed (for example, see Patent Document 1).

  Thus, sake lees have various effects, but development of a preparation with higher anti-aging action, hair-restoration action, and anti-obesity action is desired.

JP-A-10-130121

  An object of the present invention is to solve the conventional problems and achieve the following objects. That is, the present invention provides an anti-aging agent having an excellent anti-aging effect, a hair-restoring agent having an excellent hair-growth effect, an anti-obesity agent having an excellent anti-obesity effect, a cosmetic containing at least one of these, and a food and drink. With the goal.

Means for solving the problems are as follows. That is,
<1> An anti-aging agent characterized by containing a yeast fermented product obtained by fermenting a sake lees extract with yeast.
<2> The anti-aging agent according to <1>, wherein the yeast is Saccharomyces genus Verona.
<3> A hair restorer comprising a yeast fermented product obtained by fermenting a sake lees extract with yeast.
<4> The hair restorer according to <3>, wherein the yeast is Saccharomyces genus Verona.
<5> An anti-obesity agent comprising a fermented yeast product obtained by fermenting a sake lees extract with yeast.
<6> The antiobesity agent according to <5>, wherein the yeast is Saccharomyces genus Verona.
<7> For anti-aging including the anti-aging agent according to any one of <1> to <2>, for hair growth including the hair-restoring agent according to any one of <3> to <4>, and < 5. A cosmetic comprising at least one selected from anti-obesity agents including the anti-obesity agent according to any one of 5> to <6>.
<8> For anti-aging including the anti-aging agent according to any one of <1> to <2>, for hair growth including the hair-restoring agent according to any one of <3> to <4>, and < It is at least 1 sort (s) selected from the anti-obesity agent containing the anti-obesity agent in any one of 5> to <6>.

  According to the present invention, the conventional problems described above are solved, and an anti-aging agent excellent in anti-aging action, a hair-restoring agent excellent in hair-restoring action, an anti-obesity agent excellent in anti-obesity action, and a makeup containing at least one of these And food and drinks can be provided.

(Anti-aging agent, hair restorer, and anti-obesity agent)
The anti-aging agent of this invention contains the yeast fermented material which fermented the sake lees extract with the yeast, and also contains another component as needed.
The hair restorer of the present invention contains a fermented yeast product obtained by fermenting a sake lees extract with yeast, and further contains other components as necessary.
The anti-obesity agent of the present invention contains a fermented yeast product obtained by fermenting a sake lees extract with yeast, and further contains other components as necessary.

  Here, the anti-aging agent, the hair-restoring agent, and the anti-obesity agent are less likely to cause harm to human health, and in normal social life, refer to those taken by oral or gastrointestinal administration It is not limited to food, pharmaceuticals, quasi-drugs, etc. in administrative categories, but includes, for example, a wide range of functional foods, quasi-drugs, pharmaceuticals, etc. taken orally .

<Yeast fermentation product>
The yeast fermented product is a yeast fermented product obtained by fermenting the sake lees extract with yeast (hereinafter, “yeast fermented product of sake extract”) and further contains other components as necessary. The yeast fermented product of the sake lees extract can be used alone as an anti-aging agent, hair restorer, and anti-obesity agent, and can further contain other components as necessary.

<< Yeast Fungus >>
There is no restriction | limiting in particular as said yeast, According to the objective, it can select suitably, Bacteria etc. which can produce alcohol by fermentation are mentioned.

  Examples of the yeast species include, for example, the genus Saccharomyces (Saccharomyces cerevisiae), the genus Saccharomyces cerevisiae (Saccharomyces cerevisiae), ) And the like. Among these, Saccharomyces veronae is preferable because it proliferates efficiently and the obtained fermented product has a strong activity.

<< Liquor extract >>
The sake lees extract is an extract obtained by extracting sake lees with a solvent.

  There is no restriction | limiting in particular as said solvent, According to the objective, it can select suitably, For example, water, a water-soluble organic solvent, etc. are mentioned. Among these, water is preferable from the viewpoint of not inhibiting the activity of the yeast.

-Sake lees-
The sake lees are solids that remain after squeezing the moromi used in the production of sake.
There is no restriction | limiting in particular as said liquor, According to the objective, it can select suitably, For example, sake, shochu, awamori, etc. are mentioned.

  A commercial item can be used as said sake lees, for example, white peony liquor (product made from white peony brewing), etc. are mentioned.

[Method of preparing sake lees]
The method for preparing the sake lees is not particularly limited, and conventional methods for preparing sake lees can be used. For example, brown rice is made into white rice and then steamed to make steamed rice. There is a method of adding sake lees, putting sake yeast therein, producing moromi and then squeezing the moromi to obtain sake lees that are solids.

[Method for preparing sake lees extract]
There is no restriction | limiting in particular as a preparation method of the said sake lees extract, According to the objective, it can select suitably, For example, the method of suspending sake lees in a solvent etc. are mentioned.

[Method for preparing yeast fermented product]
Examples of the method for preparing the yeast fermented product include the following methods. 5 g of sake lees prepared per 100 g of water was added and mixed with stirring to obtain a liquor extract, and then a high pressure steam sterilizer (device name: MLS-3751-PJ, manufactured by Panasonic Corporation) at 110 ° C. for 5 minutes. ), Autoclave and cool after sterilization. After cooling to about room temperature, 0.1 g of yeast (species name: Saccharomyces Verona, in-house culture) is added, and shaking culture is performed at 30 ° C. for 16 to 24 hours. After culturing, sterilize by autoclaving at 110 ° C. for 5 minutes, and filter using diatomaceous earth to obtain a yeast fermentation product.

  There is no restriction | limiting in particular as content of the said yeast fermented material, According to the objective, it can select suitably, For example, 0.05 mL / mL or more with respect to an anti-aging agent, a hair restorer, and an anti-obesity agent 1.0 mL / mL or less is preferable, and 0.2 mL / mL or more and 0.8 mL / mL or less is more preferable.

  The usage form of the yeast fermented product is not particularly limited and can be appropriately selected according to the purpose. Examples thereof include a powder material obtained by freeze-drying the filtrate.

<< Other ingredients >>
The other components are not particularly limited and may be appropriately selected depending on the usage form of the anti-aging agent, hair restorer, and anti-obesity agent. For example, plant extracts, pharmacologically acceptable carriers Etc.

-Plant extract-
The plant extract is an extract from a plant.

  Examples of the plant include a thistle, an acha, an opium, an aloe bear, a ginkgo, a fennel, a turmeric, a common beetle, a larva, an age, an elephant, a life-prolonging grass, a yellow spirit, an oat, an ogon, an oak, a barley, a hypericum grass, a moth, a chamomile, and a licorice. , Kidachi Aloe, Gymnema, Cabbage, Jade Bamboo, Kiraya, Gold and Silver Flower, Chrysanthemum, Chrysanthemum, Red Ginseng, Ginseng, Kumagusu, Mulberry, Laurel Leaves, Akiko, Gentiana, Wheat, Rice, Burdock, Sesame, Salvia, Sanza, Shiso, Sanshishi, Sanche, Yam, Yamayaku, Shiitake mushrooms, Purple candy, Glaze, Car forage, Ten ginseng, White ginger, Horsetail, Stevia, Cyprus, Senna, Assembly, Buckwheat, Radish, Taiso, Soy, Tamarind, Cod, Chimpi, Green tea , Tenninka, Tokachi, Tochu, Cordyceps, Corn, Sashimi, Ginseng, Shinobyu, Parsley, Beach windbreak, Mamellisu, himematsu mushroom, bilberry, loquat, bukuro, grape, blueberry, loofah, hematin, linden tree, peony, hops, pine needles, peach, melissa, yucca, yokoinin, mugwort, rye, lakanka, green tea, apple, rooibos, lucas, Examples include ganoderma, reindeer grass, rose hips, rosemary, and bitumen.

  The plant extract can be easily obtained by a method generally used for plant extraction. In addition, the extract from the plant includes a plant extract, a dried product obtained by drying a diluted solution of the extract, or a crude product or a purified product thereof.

  The plant extraction raw material is not particularly limited and may be appropriately selected depending on the intended purpose. For example, the plant leaves, stems, flowers (buds), seeds (these are referred to as above-ground parts), roots Etc. can be used.

  The plant as the raw material for extraction can be obtained by drying or pulverizing it as it is or using a crusher and subjecting it to solvent extraction. Drying may be performed in the sun or using a commonly used dryer. The plant may be used as a raw material for extraction after pretreatment such as degreasing with a nonpolar solvent such as hexane or benzene. In addition, the extraction process by the polar solvent of a plant can be performed efficiently by performing pretreatments, such as degreasing.

As the solvent used for the extraction, it is preferable to use water, a hydrophilic organic solvent, or a mixed solvent thereof at a temperature from room temperature to the boiling point of the solvent.
Examples of water that can be used as the extraction solvent include pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as water that has been subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. The water that can be used as the extraction solvent includes purified water, hot water, ion exchange water, physiological saline, phosphate buffer, phosphate buffered saline, and the like.

Examples of the hydrophilic organic solvent include lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene glycol, propylene glycol, Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycerin, and a mixed solvent of these hydrophilic organic solvents and water can be used.
In addition, when using the mixed solvent of the said water and the said hydrophilic organic solvent, in the case of a lower alcohol, 1 mass part-90 mass parts with respect to 10 mass parts of water, and in the case of a lower aliphatic ketone, water 10 It is preferable to add 1 part by mass to 40 parts by mass with respect to part by mass. In the case of polyhydric alcohol, it is preferable to add 1 part by mass to 90 parts by mass with respect to 10 parts by mass of water.

  In the present invention, when extracting an extract from a plant as an extraction raw material, it is not necessary to adopt a special extraction method, and the extraction can be performed using an arbitrary extraction device at room temperature or under reflux heating.

  Specifically, a plant as an extraction raw material is put into a treatment tank filled with an extraction solvent, and further, if necessary, after standing for 30 minutes to 2 hours with occasional stirring to elute soluble components. The solid matter is removed by filtration, the extraction solvent is distilled off from the obtained extract, and the extract is obtained by drying. The amount of the extraction solvent is usually 5 to 15 times (mass ratio) of the extraction raw material. The extraction conditions are usually about 1 to 4 hours at 50 to 95 ° C. when water is used as the extraction solvent. Moreover, when the mixed solvent of water and ethanol is used as an extraction solvent, it is normally 30 minutes-about 4 hours at 40 to 80 degreeC. In addition, the extract obtained by extracting with a solvent can be used as it is as a plant extract as long as the extraction solvent is highly safe.

  The obtained plant extract is diluted, concentrated, dried, or purified according to a conventional method to obtain a diluted or concentrated solution of the extract, a dried product of the extract, or a crudely purified product or purified product thereof. Processing may be performed.

  Since the plant extract contains an extract from the plant, it can exhibit various physiological functions of the extract from the plant.

  The carrier is not particularly limited as long as the effects of the present invention are not impaired. For example, when the active ingredient is used in various dosage forms, it can be appropriately selected depending on the dosage form. The dosage form is not particularly limited as long as the effects of the present invention are not impaired, and can be appropriately selected depending on the administration method. For example, oral solids (tablets, coated tablets, granules, powders, capsules) , Lozenges, etc.), oral solutions (internal solutions, syrups, elixirs, etc.).

As the oral solid preparation, for example, additives such as excipients, binders, disintegrants, lubricants, colorants, flavoring / flavoring agents, and the like can be added according to a conventional method.
Examples of the excipient include lactose, sucrose, sodium chloride, glucose, starch, calcium carbonate, kaolin, microcrystalline cellulose, and silicic acid.

Examples of the additive include a binder, a disintegrant, a lubricant, a colorant, and a flavoring / flavoring agent.
Examples of the binder include water, ethanol, propanol, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, hydroxypropylcellulose, hydroxypropyl starch, methylcellulose, ethylcellulose, shellac, calcium phosphate, polyvinylpyrrolidone and the like. It is done.
Examples of the disintegrant include dry starch, sodium alginate, agar powder, sodium hydrogen carbonate, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, and lactose.
Examples of the lubricant include purified talc, stearate, borax, and polyethylene glycol.
Examples of the colorant include titanium oxide and iron oxide.
Examples of the flavoring / flavoring agent include sucrose, orange peel, citric acid, tartaric acid and the like.

As the oral solution, for example, additives such as a taste-masking / flavoring agent, a buffering agent, a stabilizer and the like can be added and produced by a conventional method. Here, the additive is not particularly limited as long as the effects of the present invention are not impaired, and can be appropriately selected according to the purpose.
Examples of the flavoring / flavoring agent include sucrose, orange peel, citric acid, tartaric acid and the like.
Examples of the buffer include sodium citrate.
Examples of the stabilizer include tragacanth, gum arabic, and gelatin.

  The anti-aging agent, hair-restoring agent, and anti-obesity agent of the present invention are preferably applied to humans, but may be applied to animals other than humans as long as their respective effects are exhibited. You can also

(Cosmetics)
The cosmetic contains at least one selected from the anti-aging agent, hair restorer, and anti-obesity agent of the present invention, and further contains other components as necessary.
The cosmetic of the present invention has an anti-aging effect, a hair-growth effect, and an anti-obesity effect through a transglutaminase-1 production promoting effect, a hair papillary cell growth promoting effect, and a cAMP phosphodiesterase activity inhibiting effect by a yeast fermentation product of sake lees extract. At least one of the above can be improved.

  The content of at least one of the anti-aging agent, hair restorer, and anti-obesity agent is not particularly limited and may be appropriately selected depending on the application. Is preferably 10 mg / g or more, more preferably 200 mg / g or more, and particularly preferably 500 mg / g or more.

<Other ingredients>
The other components are not particularly limited as long as they are used in cosmetics, and can be appropriately selected according to the purpose. For example, astringents, bactericides, antibacterial agents, ultraviolet absorbers, humectants, Examples include cell activators, fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances and the like. These may be used alone or in combination of two or more.
There is no restriction | limiting in particular as content of the said other component, According to the objective, it can select suitably.

(Food)
The food or drink includes at least one selected from the anti-aging agent, hair restorer, and anti-obesity agent of the present invention, and further includes other components as necessary.
The food / beverage product of the present invention has an anti-aging effect, a hair-growth effect, and an anti-obesity effect through a transglutaminase-1 production promoting action, a hair papillary cell growth promoting action, and a cAMP phosphodiesterase activity inhibiting action by a yeast fermentation product of a sake lees extract. At least one of the above can be improved. In addition, in this invention, food / beverage products mean the wide food / beverage products aiming at anti-aging, hair growth promotion, and obesity prevention.

  There is no restriction | limiting in particular as said food-drinks, Although it can select suitably according to the objective, Beverages, such as a tea drink, a soft drink, a carbonated drink, a nutritive drink, a fruit drink, a lactic acid drink; Ice cream, ice Frozen confectionery such as sorbet and shaved ice; noodles such as buckwheat, udon, harusame, gyoza skin, sushi mai, Chinese noodles and instant noodles; rice cake, candy, gum, chocolate, tablet confectionery, snack confectionery, biscuits, jelly, jam, cream , Baked confectionery, bakery and other confectionery; crab, salmon, clam, tuna, sardine, shrimp, bonito, mackerel, whale, oyster, saury, squid, red scallop, scallop, abalone, sea urchin, crabs, tocobushi, etc .; Processed fishery and livestock products such as ham, sausage; dairy products such as processed milk and fermented milk; salad oil, tempura oil, margarine, mayonnaise Oils and processed foods such as shortenings, whipped creams, dressings, etc .; seasonings such as sauces, sauces; curry, stew, oyakodon, rice cakes, miscellaneous cooking, Chinese rice cakes, tempura, eel rice cakes, hayashi rice, oden, marbodorf, Retort pouch foods such as beef bowl, meat sauce, egg soup, omelet rice, dumplings, shumai, hamburger and meatballs; various forms of health foods and dietary supplements.

  The content of at least one selected from the anti-aging agent, hair restorer, and anti-obesity agent is not particularly limited and may be appropriately selected depending on the application. Preferably, 10 mg / g or more is preferable, 200 mg / g or more is more preferable, and 500 mg / g or more is particularly preferable.

<Other ingredients>
There is no restriction | limiting in particular as said other component, According to the objective, it can select suitably.

<Application>
The anti-aging agent, hair-restoring agent, anti-obesity agent of the present invention, and cosmetics and foods and drinks are excellent in anti-aging action, hair-restoring action, and anti-obesity action. Moreover, it can use suitably for the cosmetics containing at least any of these, and food-drinks.

  Examples of the present invention will be described below, but the present invention is not limited to these examples.

(Preparation Example 1)
As the sake lees, white peony liquor (manufactured by white peony brewing) was used. 5 g of the sake lees were suspended in 100 g of water to obtain a liquor extract.

(Preparation Example 2)
The prepared sake lees extract is autoclaved with a high-pressure steam sterilizer (device name: MLS-3751-PJ, manufactured by Panasonic Corporation) at 110 ° C. for 5 minutes, sterilized, and then cooled. After cooling to about room temperature, 0.1 g of yeast (species name: Saccharomyces Verona, in-house culture) is added, and shaking culture is performed at 30 ° C. for 16 to 24 hours. After completion of the culture, the mixture was sterilized by autoclaving again at 110 ° C. for 5 minutes and filtered using diatomaceous earth to obtain a yeast fermentation product (liquid).
The obtained yeast fermented product was freeze-dried with a freeze-dryer (device name: FDU-2100, manufactured by EYELA) to be powdered.

  Next, using the prepared sake lees extract and the yeast fermentation powder (test sample) of the sake lees extract, “anti-aging action (transglutaminase-1 production promoting effect)”, “ Hair growth action (proliferation effect of dermal papilla cells) ”and“ anti-obesity action (cAMP phosphodiesterase activity inhibition effect) ”were evaluated. The results are shown in Tables 1 to 3 below.

Example 1
<Anti-aging effect>
<< Transglutaminase-1 production promoting action test >>
Normal human neonatal epidermal keratinocytes (trade name: NHEK, Kurashiki Spinning Co., Ltd.) were cultured for 7 days using normal human epidermal keratinocyte growth medium (KGM, Kurashiki Spinning Co., Ltd.), then Trypsine / Cells were collected with an EDTA solution (Kurashiki Boseki Co., Ltd.). The collected cells were diluted with KGM to a concentration of 1.0 × 10 ⁵ cells / mL, then seeded at 100 μL per well in a 96-well plate, and cultured for 2 days. After completion of the culture, 100 μL of a test sample diluted with KGM was added per well and cultured for 24 hours. After completion of the culture, the medium is removed from each well, 200 μL of ethanol: acetone = 1: 1 is added to the cells, and the cells are allowed to stand at 4 ° C. for 30 minutes. Cells were fixed on a plate, and transglutaminase-1 expressed on the cell surface was quantitatively analyzed by ELISA using a monoclonal anti-human transglutaminase-1 antibody (Biomedical Technologies. Inc.). The average value of the transglutaminase expression promotion rate was obtained and used as an index of the anti-aging effect. The results are shown in Table 1 below. The method for calculating the transglutaminase expression promotion rate is as follows.

Transglutaminase-1 production promotion rate (%) = (A / B) × 100 Formula 1
A: Absorbance at a wavelength of 405 nm when a test sample is added B: Absorbance at a wavelength of 405 nm when no test sample is added

(Example 2)
<Hair growth action>
<< Test for promoting dermal papilla cell proliferation >>
Normal human hair hair papilla cells (trade name: HFDPC, manufactured by Toyobo Co., Ltd.) were cultured for 7 days using a hair papilla cell growth medium (manufactured by Toyobo Co., Ltd.) containing 1% FCS and a growth increasing agent, and then Trypsine. The cells were collected with an / EDTA solution (manufactured by Toyobo Co., Ltd.). The collected cells were diluted to a concentration of 1.0 × 10 ⁴ cells / mL using 10% FBS-containing DMEM (manufactured by Nissui Pharmaceutical Co., Ltd.), and then seeded at 200 μL per well in a collagen-coated 96-well plate And cultured for 3 days. After the culture, the medium was removed, and 200 μL of a test sample dissolved in serum-free DMEM was added to each well, followed by further culturing for 4 days. After completion of the culture, 100 μL of serum-free DMEM medium containing MTT (3- (4,5-Dimethyl-2-thiazolyl) -2,5-diphenyltetrazole Bromide) prepared to a final concentration of 0.4 mg / mL was added to each well. . After culturing for 2 hours, blue formazan produced in the cells was extracted with 100 μL of 2-propanol. After extraction, absorbance at a wavelength of 570 nm and absorbance at a wavelength of 650 nm are measured using a microplate reader (device name: μQuant, manufactured by Bio-Tek), and the difference between the two values is defined as the amount of blue formazan produced. The average value for 6 samples was calculated from the nipple cell proliferation promotion rate. The results are shown in Table 2. In addition, the calculation method of the hair papilla cell proliferation promotion rate is as follows.

・ Propagation rate of dermal papilla cells (%) = (A / B) × 100 Formula 2
A: Blue formazan production when test sample is added B: Blue formazan production when no test sample is added

Example 3
<Anti-obesity action>
<< cAMP phosphodiesterase activity inhibition test >>
To a V-bottom test tube with a lid (Asahi Glass Co., Ltd.) containing 5 mmol / L magnesium chloride-containing 50 mmol / L Tris-HCl buffer (Wako Pure Chemical Industries, Ltd., pH 7.5) 0.2 mL, 2.5 mg / L mL bovine serum albumin solution (trade name: albumin derived from bovine serum, product number: A2153, manufactured by Sigma-Aldrich Japan G.K.) Heart, Crude (manufactured by Sigma-Aldrich Japan G.K.) and 0.1 mL of sake lees extract or 0.05 mL of yeast fermentation product of sake lees extract were added, and the mixed solution was reacted at 37 ° C. for 5 minutes. After the reaction, 0.05 mL of 0.5 mg / mL cyclic AMP (cAMP) solution was added to the mixed solution, reacted at 37 ° C. for 60 minutes, then boiled on a boiling water bath for 3 minutes to stop the reaction. It was. Centrifugation was performed at 2,260 × g for 10 minutes at 4 ° C. with a centrifuge (device name: KUBOTA7820, manufactured by Kubota Corporation), and the supernatant was subjected to HPLC analysis under the following conditions. Further, a blank test was performed in the same manner as described above.

-Conditions for HPLC chromatography-
-Equipment used: Wakosil C18-ODS 5 μm
-Mobile phase: 1 mM TBAP in 25 mM KH ₂ PO ₄ : CH ₃ CN
= 90: 10
・ Flow rate: 1.0 mL / min
・ Detector: UV-visible spectrophotometer (measurement wavelength: 260 nm)
-ATTEN: 128

  In order to calculate the inhibition rate of cAMP phosphodiesterase activity by the test sample, the standard sample solution with a known cAMP content, the sample solution with no test sample added, and the peak area of cAMP contained in the sample solution with the test sample added, respectively. , (A), (B1), and (B2). From the obtained results, the cAMP degradation rate (C) when no test sample was added and the cAMP degradation rate (D) when the test sample was added were calculated based on the following formulas 4 and 5. The results are shown in Table 3 below.

-Degradation rate of cAMP without addition of test sample (C,%) = {(1-B1 / A)} × 100 (4)

-Degradation rate of cAMP at the time of addition of test sample (D,%) = {(1-B2 / A)} × 100 Formula 5

  Based on the respective degradation rates (C, D) calculated from the above formulas 4 and 5, the cAMP phosphodiesterase activity inhibition rate (%) is calculated by the following formula 6, and the results are shown in Table 4.

CAMP phosphodiesterase activity inhibition rate (%) = {(1-D / C)} × 100 Formula 6

(Formulation Example 1)
-Lotion-
A lotion having an anti-aging action having the following composition was produced by a conventional method.
・ Glycerin ... 3.0g
・ 1,3-Butylene glycol ・ ・ ・ 3.0g
・ Oleic acid polyoxyethylene sorbitan (20E.0) 0.5g
・ Methyl paraoxybenzoate 0.15 g
・ Citric acid ... 0.1g
・ Sodium citrate ... 0.1g
・ Vitamin E ... 0.1g
・ Camelis extract ... 1.0g
・ Parsley-derived antioxidant substance 0.5g
・ Fragrance ... 0.05g
-Yeast fermentation product (liquid) of the sake lees extract of Preparation Example 2 80.0 mL
・ Purified water: remaining amount (total amount is 100 mL)

(Formulation Example 2)
-Hair tonic-
A hair tonic having a hair growth action having the following composition was produced by a conventional method.
・ Pyridoxine hydrochloride ... 0.1g
・ Resorcin ・ ・ ・ 0.01g
・ D-pantothenyl alcohol ... 0.1g
・ Dipotassium glycyrrhizinate ... 0.1g
・ L-Menthol 0.05g
・ 1,3-Butylene glycol ・ ・ ・ 4.0g
・ Carrot extract ... 0.5g
・ Ethanol ... 25.0g
-Yeast fermentation product (liquid) of the sake lees extract of Preparation Example 2 50 mL
-Fragrance-appropriate amount-Purified water-remaining amount (total amount is 100.0 mL)

(Formulation Example 3)
-Shampoo-
A shampoo (cream shampoo) having a hair-growing action having the following composition was produced by a conventional method.
・ Polyoxyethylene alkyl ether sodium sulfate 30.0 g
・ Polyoxyethylene alkyl ether ammonium sulfate ... 20.0g
・ Palm oil fatty acid amidopropyl betaine ... 6.0g
・ Palm oil fatty acid modiethanolamide: 4.0 g
・ Ethylene glycol distearate ... 2.0g
・ Preservative (methyl paraoxybenzoate) ... 0.15g
・ Muguroji extract ... 0.2g
・ Cotton extract ... 0.5g
・ Oat extract ... 0.3g
・ Rosemary extract 0.5g
・ Perfume 0.01g
・ 1,3-Butylene glycol ・ ・ ・ 3.0g
-Yeast fermentation product (liquid) of the sake lees extract of Preparation Example 2 (remaining amount (total amount is 100.0 mL))

(Formulation Example 4)
-Rinse-
A rinse having a hair-growth effect having the following composition was produced by a conventional method.
・ Stearyltrimethylammonium chloride 1.5g
・ Polyoxyethylene cetyl ether ... 1.0g
・ Cetyl alcohol: 2.0g
・ Octyldodecanol ・ ・ ・ 1.0g
・ Cationized cellulose: 0.5g
・ Propylene glycol ... 5.0g
-Yeast fermentation product (liquid) of the sake lees extract of Preparation Example 2 40 mL
・ Muguroji extract ... 0.2g
・ Cotton extract ... 0.5g
・ Oat extract ... 0.3g
・ Rosemary extract 0.5g
・ Fragrance ... 3.0g
・ Purified water: remaining amount (the total amount is 100.0 mL)

  The anti-aging agent, hair-restoring agent, anti-obesity agent of the present invention, and cosmetics and foods and drinks are excellent in anti-aging action, hair-restoring action, and anti-obesity action. And it can utilize suitably for the cosmetics containing at least any of these, and food-drinks.

Claims (8)

  An anti-aging agent comprising a fermented yeast product obtained by fermenting a liquor extract with yeast.   The anti-aging agent according to claim 1, wherein the yeast is Saccharomyces genus Verona.   A hair restorer comprising a yeast fermented product obtained by fermenting a sake lees extract with yeast.   The hair restorer according to claim 3, wherein the yeast is Saccharomyces genus Verona.   An antiobesity agent comprising a yeast fermentation product obtained by fermenting a sake lees extract with yeast.   The anti-obesity agent according to claim 5, wherein the yeast is Saccharomyces genus Verona.   The anti-aging agent comprising the anti-aging agent according to any one of claims 1 to 2, the hair restoring agent comprising the hair restoring agent according to any one of claims 3 to 4, and any one of claims 5 to 6. A cosmetic comprising at least one selected from anti-obesity agents including an anti-obesity agent.   The anti-aging agent comprising the anti-aging agent according to any one of claims 1 to 2, the hair restoring agent comprising the hair restoring agent according to any one of claims 3 to 4, and any one of claims 5 to 6. A food or drink comprising at least one selected from anti-obesity agents including an anti-obesity agent.